CN201413328Y - Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus - Google Patents
Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus Download PDFInfo
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- CN201413328Y CN201413328Y CN 200920026848 CN200920026848U CN201413328Y CN 201413328 Y CN201413328 Y CN 201413328Y CN 200920026848 CN200920026848 CN 200920026848 CN 200920026848 U CN200920026848 U CN 200920026848U CN 201413328 Y CN201413328 Y CN 201413328Y
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- virus
- swine flu
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- antibody
- colloidal gold
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Abstract
The utility model relates to a colloidal gold test paper strip for rapidly testing swine flu H1N1 virus, which is characterized by comprising a substrate, and a coated film is stuck at the middle parton the substrate; the middle part of the coated film is provided with a detection line coated with swine flu H1N1 virus antibody and a control line coated with anti-mouse antibody, and a glass fibermembrane coated with the gold-labeled antibody and a piece of water absorption paper are respectively stuck at the left and the right of the coated film; and a sample pad is stuck on the glass fiber membrane coated with the gold-labeled antibody. By utilizing the modern international most advanced colloidal gold immuno-chromatographic technology, the colloidal gold test paper strip for testing swine flu H1N1 virus is provided for self-testing of local farmers, grass roots and the like, has the advantages of strong specificity, high sensitivity, rapid testing speed and the like, is free from instrument and equipment, and is low in cost as well as simple and convenient for operation, thus being widely applied to the local farmers, the grass roots and individual for testing the swine flu H1N1virus.
Description
Technical field
The utility model belongs to Preventive Veterinary Medicine check field, specifically relates to a kind of colloidal gold strip of fast detecting swine flu H1N1 virus.
Background technology
Swine influenza virus (swine influenza virus, SIV) belong to the A of orthomyxoviridae family type Influenza Virus, the pig that can cause different days, sex and kind is taken place acute, hot and height contact respiratory infectious disease, and it is a feature with burst, high heat, cough, expiratory dyspnea, depletion and death clinically.U.S.'s reported first in 1918 breaking out of swine flu, nineteen thirty shope isolates H1N1 hypotype swine influenza virus first from the pig body.Up to now, swine flu has spread all over U.S., Europe, Asia and Africa etc. all over the world.At present, the SIV that has been found that comprises hypotypes such as H1N1, H1N2, H1N7, H3N2, H3N6, H4N6, H5N1 and H9N2.But with classical H1N1, bird H1N1 and class people H3N2 hypotype strain are main.China's swine flu in 1969 by the Taiwan reported first, from swinery, be separated to H1N1 hypotype swine influenza virus in 1991 first.Guo Yuanji (1985), Ni Hanzhong (2000), Zhang Suhua (2002), Li Haiyan (2003) etc. are to having done serosurvey in China different regions, and the result shows that with increasing progressively of time, China's swine flu positive rate increases.
At present, detection method to influenza virus mainly contains viral separation, serodiagnosis and molecular biology method, but viral disengaging time is long, the requirement condition height, separation rate is low, and gather the patients acuity phase and convalescence paired sera carry out TPPA and confirm, can not in time diagnose again, therefore, these methods in use are very restricted, also not seeing has available device or other testing tool.
Summary of the invention
The purpose of this utility model provides a kind of colloidal gold strip of fast detecting swine flu H1N1 virus, and it can satisfy the demand of prior art.
A kind of colloidal gold strip of fast detecting swine flu H1N1 virus, it is characterized in that an end liner is arranged, the sticking obedient coated film in this middle part above end liner, this coated film middle part have a bag by swine flu H1N1 virus detection of antibodies line and a bag by the control line of anti-mouse antibody, the glass fibre membrane and the thieving paper of a sticking obedient coated with gold labeling antibody respectively about coated film top; Paste a sample pad above the glass fibre membrane of coated with gold labeling antibody.
The utility model utilizes modern international state-of-the-art colloidal gold immunochromatographimethod technology, the colloidal gold strip of the detection swine flu H1N1 virus that a kind of terrain peasant household, basic unit usefulness such as test oneself is provided, this test strips has high specificity, highly sensitive, advantage such as detection speed is fast, and need not use instrument and equipment, with low cost, easy and simple to handle, can be widely used in terrain peasant household, basic unit and individual detection for the swine flu H1N1 virus.
Description of drawings
Accompanying drawing is a vertical profile structural representation of the present utility model.
Embodiment
The colloidal gold strip of the utility model fast detecting swine flu H1N1 virus, as shown in the figure, one end liner 1, the sticking obedient coated film 4 in this middle part above end liner 1 are arranged, these coated film 4 middle parts have a bag by swine flu H1N1 virus detection of antibodies line 6 and a bag by the control line 7 of anti-mouse antibody, the glass fibre membrane 3 and the thieving paper 5 of a sticking obedient coated with gold labeling antibody respectively about coated film 4 top; Paste a sample pad 2 above the glass fibre membrane 3 of coated with gold labeling antibody.
Manufacture method of the present utility model is as follows:
With BALB/C mice immune swine influenza H1N1 virus, get its splenocyte and the SP2/O Fusion of Cells is set up tumor cell strain, screen, get oncocyte and be injected in the BALB/C mice abdominal cavity, make it produce ascites.Extract mouse ascites and carry out the purifying screening, acquisition can be used for colloid gold label with the monoclonal antibody of swine flu H1N1 virus generation neutralization reaction.
During preparation coated film 4, the carbonate buffer solution (PBS) of 0.05M, pH9.6 is used the 0.22u membrane filtration, make bag and be cushioned liquid.In addition 20g bovine serum albumin(BSA) (BSA) and 20g skimmed milk are dissolved in the PBS 0.22u membrane filtration of 1000ml0.01M, pH7.0, make the sealing working fluid.The debugging Membrane jetter, be cushioned the monoclonal antibody and the anti-mouse IgG polyclonal antibody of liquid dilution anti-swine flu H1N1 virus with above-mentioned bag, line on the nitrocellulose membrane middle part respectively, line-to-line is every 5mm, should be careful even, room temperature was dried 20 minutes, and the liquid that this two line is used infiltrates respectively in the nitrocellulose membrane, promptly was respectively detection line 6 and control line 7.With above-mentioned sealing working fluid with nitrocellulose membrane in 37 ℃ of sealings 60 minutes, take out and to be placed on 37 ℃ of oven dry and to handle two hours, make coated film 4.
When making the glass fibre membrane 3 of coated with gold labeling antibody, the 10g gold chloride with the dissolving of 1000ml distilled water, is made into aqueous solution; The 10g trisodium citrate with the dissolving of 1000ml distilled water, is made into aqueous solution; 13.8g sal tartari is dissolved in the 1000ml distilled water, uses the 0.22u membrane filtration, be mixed with wet chemical; 20g BSA is dissolved among the PBS of 1000ml 0.01M, pH7.0, is made into the mark cleansing solution with the 0.22u membrane filtration; With 10g BSA, 5g skimmed milk power, 0.5g NaN
3Dissolve in 1000ml 0.01M, pH 7.0 PBS with 1ml Tween-20,, be made into golden labeling antibody and preserve liquid with 0.22u membrane filtration mistake.With distilled water 1% gold chloride is diluted to 0.01%, puts electric furnace and boil, add 4 milliliter of 1% citrate three sodium, continue to boil, be shiny red up to liquid and promptly stop heating, supply dehydration after being cooled to room temperature by per 100 milliliter of 0.01% gold chloride.Outward appearance should be pure, and is bright, do not have precipitation and floating thing, is the collaurum that is fired into.Transfer the pH value to 7.6 of collaurum with the 0.1M wet chemical, the monoclonal antibody that adds the anti-swine flu H1N1 virus by 10 micrograms antibody/milliliter collaurum, mixing, left standstill 30 minutes, with 12000rpm centrifugal 30 minutes, supernatant discarded, with gained precipitation mark cleansing solution washed twice, last abandoning supernatant is preserved the liquid dissolving with the gained precipitation with the golden labeling antibody of 1/10th initial collaurum volumes, is mixed with golden labeling antibody.The labeling antibody that above-mentioned mark is good is coated on the glass fibre membrane equably, 10 square centimeters of every ml soln coating shops, and drying makes the glass fibre membrane 3 that covers golden labeling antibody.
Described end liner 1, sample pad 2 and thieving paper 5 are the general materials in this area.Above-mentioned coated film 4, the glass fibre membrane 3 that covers golden labeling antibody, end liner 1, sample pad 2 and thieving paper 5 are pasted successively, obtained test paper plate, the test strips that at last this test paper plate is cut into different in width gets final product.
The utility model can be used to detect blood sample or cotton swab etc. and handles sample, because detection line 6 and control line 7 can produce exceptional function, thereby satisfies the demand that terrain peasant household, basic unit are detected.A positive result of aubergine band respectively appears on the detection line 6 of test strips and control line 7 positions; The negative result of aubergine band only appears on nature controlling line 7 positions of test strips; The aubergine band all not occurring on the detection line 6 of test strips and control line 7 positions is null result.
Claims (3)
1. the colloidal gold strip of a fast detecting swine flu H1N1 virus, the sticking obedient coated film (4) in the middle part of it is characterized in that an end liner (1) is arranged, this end liner (1) is top, this coated film (4) middle part have a bag by swine flu H1N1 virus detection of antibodies line (6) and a bag by the control line (7) of anti-mouse IgG antibody, glue the glass fibre membrane (3) and the thieving paper (5) of an obedient coated with gold labeling antibody about coated film (4) top respectively; Paste a sample pad (2) above the glass fibre membrane of coated with gold labeling antibody (3).
2. the colloidal gold strip of fast detecting swine flu H1N1 virus according to claim 1 is characterized in that the antibody of the swine flu H1N1 virus of bag quilt in the described detection line (6) is highly purified anti-monoclonal antibody and/or polyclonal antibody.
3. the colloidal gold strip of fast detecting swine flu H1N1 virus according to claim 1 is characterized in that having in the glass fibre membrane (3) of described coated with gold labeling antibody the monoclonal antibody by the anti-swine flu H1N1 virus of colloid gold particle mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920026848 CN201413328Y (en) | 2009-06-02 | 2009-06-02 | Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920026848 CN201413328Y (en) | 2009-06-02 | 2009-06-02 | Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201413328Y true CN201413328Y (en) | 2010-02-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200920026848 Expired - Lifetime CN201413328Y (en) | 2009-06-02 | 2009-06-02 | Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus |
Country Status (1)
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| CN (1) | CN201413328Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102241768A (en) * | 2010-05-14 | 2011-11-16 | 中国科学院上海生命科学研究院 | Antibody against hemagglutinin of influenza A H1N1 virus |
| CN112063765A (en) * | 2020-11-11 | 2020-12-11 | 北京欣颂生物科技有限公司 | Nucleic acid antibody dual-detection virus kit and preparation method thereof |
| CN112280901A (en) * | 2020-11-11 | 2021-01-29 | 北京欣颂生物科技有限公司 | Application of improved nucleic acid detection technology in preparation of virus detection kit |
-
2009
- 2009-06-02 CN CN 200920026848 patent/CN201413328Y/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102241768A (en) * | 2010-05-14 | 2011-11-16 | 中国科学院上海生命科学研究院 | Antibody against hemagglutinin of influenza A H1N1 virus |
| CN102241768B (en) * | 2010-05-14 | 2013-12-25 | 中国科学院上海生命科学研究院 | Antibody against hemagglutinin of influenza A H1N1 virus |
| CN112063765A (en) * | 2020-11-11 | 2020-12-11 | 北京欣颂生物科技有限公司 | Nucleic acid antibody dual-detection virus kit and preparation method thereof |
| CN112280901A (en) * | 2020-11-11 | 2021-01-29 | 北京欣颂生物科技有限公司 | Application of improved nucleic acid detection technology in preparation of virus detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Heze Puen Pharmaceutical Co., Ltd. Assignor: Qingdao continet Pharmaceutical Co. Ltd.|Qingdao boite biopharmaceutical Co. Ltd. Contract record no.: 2011370000088 Denomination of utility model: Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus Granted publication date: 20100224 License type: Exclusive License Record date: 20110412 |
|
| C56 | Change in the name or address of the patentee |
Owner name: QINGDAO KDN PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME: QINGDAO CONTINENT BIOTECH CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 266061 Shandong Province, Qingdao high tech park by the road No. 29 Shandong High Building Room 606 Co-patentee after: Qingdao Boite Biopharmaceutical Co., Ltd. Patentee after: Qingdao KDN Pharmaceutical Co., Ltd. Address before: 266061 Shandong Province, Qingdao high tech park by the road No. 29 Shandong High Building Room 606 Co-patentee before: Qingdao Boite Biopharmaceutical Co., Ltd. Patentee before: Qingdao Continent Biotech Co., Ltd. |
|
| C56 | Change in the name or address of the patentee |
Owner name: QINGDAO WEILAN BIOLOGY CO., LTD. Free format text: FORMER NAME: QINGDAO KDN PHARMACEUTICAL CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 266111 Shandong city of Qingdao province Chengyang Qingda Industrial Park, the first northbound dual Patentee after: QINGDAO VLAND BIOLOGICAL CO., LTD. Patentee after: QINGDAO VLAND BIOLOGICAL PRODUCTS CO., LTD. Address before: 266061 Shandong Province, Qingdao high tech park by the road No. 29 Shandong High Building Room 606 Patentee before: Qingdao KDN Pharmaceutical Co., Ltd. Patentee before: Qingdao Boite Biopharmaceutical Co., Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100224 |