CN201852815U - Lab VIEW-based nanometer passageway monomolecular detection system - Google Patents
Lab VIEW-based nanometer passageway monomolecular detection system Download PDFInfo
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- CN201852815U CN201852815U CN2010201696436U CN201020169643U CN201852815U CN 201852815 U CN201852815 U CN 201852815U CN 2010201696436 U CN2010201696436 U CN 2010201696436U CN 201020169643 U CN201020169643 U CN 201020169643U CN 201852815 U CN201852815 U CN 201852815U
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Abstract
The utility model provides a Lab VIEW-based nanometer passageway monomolecular detection system which comprises a data acquiring device, a data processing device, a data result displaying device, a data filtering device and a data memorizing and searching device, wherein the data acquiring device comprises a data acquiring plate card, an amplifier connected with the data acquiring plate card and a sensor connected with the amplifier; the data processing device is used for processing date from the data acquiring device; the data result displaying device comprises a waveform outputting unit connected with the data processing device and a waveform positioning, replaying and memorizing unit connected with the waveform outputting unit; the data filtering device is used for checking and prompting all acquired date according to a preset data acquiring rule for a user to redisplay a data statistics result without incorrect data; and the data memorizing and searching device comprises a data memorizing unit connected with the data filtering device and a data searching and deriving unit connected with the data memorizing unit. The system is simple in structure, low in cost for manufacturing the whole system, strong in pertinence, and simple to operate.
Description
Technical field
The utility model mainly is the aspects such as data acquisition, record and processing about nanochannel (Nanopore) technology about nanochannel (Nanopore) technical field.
Background technology
Nanochannel (Nanopore) technology be one in conjunction with subjects such as biophysics, molecular biology and biology sensors in the novel nano detection technique of one.
What be used in most of laboratories at present to finish nanochannel (Nanopore) data acquisition-and-recording is patch-clamp (Patch Clamp) technology.This technology is a kind of by forming the method that closely contact between microelectrode and the cell membrane, the microelectrode technology that employing voltage clamp or current clamp technology write down the electrical activity of ion channel on the biological membrane.Experimental signal is input in the Digidata series digital to analog converter by Axopatch series patch clamp amplifier, carries out data recording and analysis by " single " among the business software Pclamp of maturation.In this technology, incident (Event) is single channel data recording and essential element of analysis, and by analyzing the current amplitude and the duration of incident, the experimenter just can obtain information such as the structure, size of detected material.
The exploitation of this technology with the development at be animal or human's body cells such as various myocytes, neuron, endocrine cell haemocyte, bacterium, fungi and vegetable cell can not satisfy the application of the used plane bilayer of this experimental technique fully.Patch Clamp technology can't provide current blocking time and electric current descent amplitude exactly in addition, makes follow-up data handle inconvenience to some extent.In addition, its required instrument, data acquisition software and check and analysis system cost costliness and experiment purposes are more single.
Background technology of the present utility model also can be with reference to following document:
1. Liu Zhen is big.Practical patch clamp technique.ISBN?7-80121-777-2/R.770；
2. wear light, Liu Peng, Zhang Ying.Pipeline leakage remote monitoring system based on the LabVIEW shared variable.Instrumental technique, 2009,1 (15);
3. Xu Hong is female, Fang Fang, Cui Junliang etc.Energy spectrum analysis system design based on LabVIEW.Nuclear technology, 2009,1 (Vol.32, No.1);
4. Chen Qing training, intercalation treasured is auspicious, Yang Yuguang etc.Hyperchannel injection machine data acquisition system (DAS) exploitation based on LabVIEW.China's plastics, 2009,2 (Vol.23, No.2).
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, and nanochannel Single Molecule Detection system a kind of simple in structure, cheap, simple to operate is provided.
For achieving the above object, the utility model adopts following technical scheme:
A kind of nanochannel Single Molecule Detection system based on LabVIEW comprises: data collector, the sensor that it comprises data collecting plate card, the amplifier that connects with data collecting plate card and connects with this amplifier; Data processing equipment connects described data collector, handles the data from data collector; The data result display device connects described data processing equipment, comprises the waveform output unit and the waveform location that connects with this waveform output unit, playback and the preservation unit that connect with described data processing equipment; Data filtering device connects described data processing equipment; And the data storage and retrieval device, connect described data filtering device, comprise data storage cell that connects with described data filtering device and data retrieval and the lead-out unit that connects with this data storage cell.
Described data collecting plate card is the PCI-6251 data collecting plate card.
Described amplifier is a kind of in forward amplifier or the sign-changing amplifier.
Described waveform output unit comprise time-the voltage pattern display module, time-the map of current display module and time-the resistance view display module.
Display voltage, electric current and resistance value simultaneously in the system that the utility model makes up, the molecular information that detects realizes monitoring in real time in instant playback the duct in.
The utility model is simple in structure, so whole system is cheap, and is simple to operate.
Below in conjunction with accompanying drawing enforcement of the present utility model and advantage are further explained.
Description of drawings
Fig. 1 is the structured flowchart of the utility model based on the nanochannel Single Molecule Detection system of LabVIEW;
Fig. 2 is the process flow diagram of the utility model data handling procedure;
Fig. 3 a, Fig. 3 b and Fig. 3 c are flow charts of the present utility model;
Fig. 4 shows waveform output unit of the present utility model;
Fig. 5 is a principle of work synoptic diagram of the present utility model.
Embodiment
Referring to Fig. 1.According to a kind of nanochannel Single Molecule Detection system 100 of the present utility model based on LabVIEW, comprise: data collector 10, it comprises data collecting plate card 11, the amplifier 12 that connects with data collecting plate card and the sensor 13 that connects with this amplifier; Data processing equipment 20 connects described data collector 10, handles the data from data collector 10; Data result display device 30 connects described data processing equipment 20, comprises the waveform output unit 31 and the waveform location that connects with this waveform output unit 31, playback and the preservation unit 32 that connect with described data processing equipment 10; Data filtering device 40, connect described data processing equipment 20, all data that collect are checked according to predefined data collecting rule, keep correct data, remove incorrect data, and point out, for the data statistics result after user's selection demonstration removal incorrect data again; And data storage and retrieval device 50, connect described data filtering device 40, comprise data storage cell 41 that connects with described data filtering device 40 and data retrieval and the lead-out unit 42 that connects with this data storage cell 41.Described data processing equipment 20, data result display device 30, data filtering device 40 and data storage and retrieval device 50 are realized by a computing machine or PC 60.
Continuation is with reference to figure 1.Described data collecting plate card 11 is PCI-6251 data collecting plate cards, the initial setting up parameter of this PCI-6251 data collecting plate card 11 comprise magnitude of voltage, current value, just control with countercharge, user profile at least a.Described amplifier 12 is a kind of in forward amplifier or the sign-changing amplifier.Described waveform output unit 31 comprise time-the voltage pattern display module, time-the map of current display module and time-the resistance view display module, be used for demonstration time-voltage pattern (T-V figure), time-map of current (T-I figure) and time-resistance view (T-R figure) respectively.Described data storage cell 51 is TXT form and TDMS form with data storage.
With reference to figure 2.Data handling procedure of the present utility model is as follows:
Step S1 is provided with data collecting plate card 11 parameters, and the user in use can be according to self needs, such as magnitude of voltage is set, current value is set, just controlling and countercharge, user profile setting etc., comes PCI allocation-6251.After configuration finishes, in experimentation, just little current signal that dna molecular is gathered during by nanochannel can be amplified, shows, analyzes, thereby obtain required result, be presented on the computer screen;
Step S2, data collecting plate card 11 acquired signal, those signals are little current signal, obtain via various kinds of sensors 13, are sent to data collecting plate card 11 after amplifier 12 amplifies;
Step S3 carries out data processing, is finished by data processing equipment 20;
Step S4, carry out data presentation and preservation, finish by data result display device 30, data filtering device 40 and data storage and retrieval device 50: to data result's display part, the utility model can be showed by T-V figure, T-I figure and T-R figure respectively, such advantage is: simultaneously display voltage, electric current and resistance value in the system of structure, hinder the molecular information that detects in the current value instant playback duct, resistance value is respective change when voltage changes, and helps the monitoring to system; To data result's storage area, huge at the experimental data amount, the characteristics that accuracy requirement is high, the utility model has adopted TXT form and TDMS form to support the derivation of data, and data retrieval function is provided simultaneously;
Step S5 carries out cycling and judges that in signal acquisition process, data are presented on the computer screen after treatment: if data acquisition is correct, then finish collection; If initial step is then returned in data acquisition incorrect (as data point coincidence, data point mistake etc.) again, gather once more.
Step S6 finishes.
In the process that little current signal that the nanochannel that the utility model provides (Nanopore) Single Molecule Detection system collects during by nanochannel (Nanopore) dna molecular or other biological molecule amplifies, shows, analyzes, not only, also combine graphical programming language LabVIEW by data collector 100 and micro current amplifier wherein 12.
Fig. 3 a and Fig. 3 b have shown two flow charts writing when the utility model utilizes LabVIEW.Fig. 3 a shows that the utility model utilizes above-mentioned hardware system to realize data acquisition countercharge and program stored block diagram, the setting analogue collection module and the data acquisition module that comprise part of data acquisition, the data analysis of data processing section and display module, data memory module and reverse control module, and the output voltage module of data output unit and setting analog output module.
Fig. 3 c shows another flow chart of writing when the utility model utilizes LabVIEW, is the concrete composition diagram of program module.By the establishment and the combination of these modules, thereby realized the function that Fig. 3 a and Fig. 3 b are showed.
Fig. 4 shows waveform output unit 31 of the present utility model, as shown in the figure, its comprise time-the voltage pattern display module, time-the map of current display module and time-three modules of resistance view display module.
Fig. 5 shows the course of work of the present utility model.A represents that galvanochemistry micro current amplifier 12, digital to analog converter 14 provide software platform to link to each other with PC 60 by LabVIEW.B, c represent in the electrochemical cell, the monomolecular detection of nanochannel.D represents detected little current signal, is presented on the data result display device 30 after amplifying.
System provided by the utility model can be used for the albumen hole that AH forms, Si3N4, the analysis and the record of the nano-pore unimolecule passage experimental data of the various ways such as solid pores that form with focused ion beam on the PET film.Because blocking action influences gas current, gas current is exaggerated through behind the micro current amplifier detected molecule, the PC that is provided by the LabVIEW platform just can be provided afterwards handle, show when electrophoretic action passes through nanochannel.In the process that PC is handled, because the superiority of the platform that LabVIEW provided, PC can compare, examine detected data, in case find misdata phenomenons such as (such as) data point overlap, data point is lost, PC just can send sense command again, again obtain data, thereby guaranteed the accuracy and the integrality of data.When data examined pass through after, just can be presented on the PC screen for the experimenter and check, and the preservation scheme is provided.Big, the demanding characteristics of data accuracy of the data data volume of being gathered in view of LabVIEW system detection platform, the utility model spy has avoided having the EXCLE memory module of maximum storage capacity restriction, has adopted the TDMS form storage of TXT document storage and LabVIEW special use.Simultaneously, the utility model can also utilize the open authority that is similar to data processing softwares such as SciLab, Matlab of LabVIEW system monitoring platform, easily passes through this class process software with detected data importing, carries out match.
The utility model at software platform that adopts and the advantage on the hardware platform is:
The software development advantage:
LabVIEW is a kind of graphical programming language, this software has the characteristics simple, easy and simple to handle, visual result of programming, on function, can customize out satisfactory software product fast according to the different requirement of researchist, programming efficiency is higher, thus for the researchist develop meet the requirements and the interface clearly nanochannel signal processing and analysis system convenience is provided.Aspect user interface, LabVIEW is integrated a large amount of controls.Can in time adjust according to researchist's demand is on-the-spot, obtain feedback fast, reduce the development time.
The hardware development advantage:
The native system hardware configuration is simple, and experimental signal enters the NI data collecting plate card after by the NI bnc connector.The transportation of hardware, installation and debugging are all very simple and convenient.Use NI hardware and, can build up the data management system that realizes concentrating test and real-time monitoring fast in conjunction with after LabVIEW and corresponding tool bag and the module.In addition,, saved the hardware development time, need not to write software driver for integrated circuit board by the seamless combination of LabVIEW and NI hardware.
LabVIEW is used for the development of virtual instrument.What is called is virtual to be exactly the function that relies on software environment realization hardware physical apparatus fully, such as signal generator, signal analysis instrument or the like.Design an apparatus with regard to the requirement that has great convenience for the user according to oneself like this, thereby more flexibility is arranged.In addition, for fear of in the building process of large scale system, because the system flexibility that the too much modularization of function causes disappearance, NI provides Labwindows/CVi simultaneously, and it is based on the code programming of C language.Native system creatively combines with detection means this separation of nanochannel with graphical programming software LabVIEW, realized the demonstration and the quick storage of data, and whole system is cheap, and is with strong points, simple to operate.Simultaneously, little current signal of can be to biomolecule gathering during by nanochannel amplifies, shows, analyzes, this system also can realize real-time monitoring, data storing and call functions such as analysis immediately current signal, thereby construct fairly simple, quick, the practical nanochannel detection system of a cover, realize the fast detecting of single molecules level.
The present invention utilizes the hardware and software platform of NI company, develop powerful, cheap data acquisition, test and analytic system, dependable performance and be easy to expansion, simultaneously display voltage, electric current and resistance value in the system of structure, hinder current value, the molecular information that detects in the instant playback duct realizes monitoring in real time, has great application space.
Certainly, more than only be concrete exemplary applications of the present utility model, protection domain of the present utility model is not constituted any limitation.In addition to the implementation, the utility model can also have other embodiment.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop within the utility model scope required for protection.
Claims (4)
1. the nanochannel Single Molecule Detection system based on LabVIEW is characterized in that, comprises:
Data collector, the sensor that it comprises data collecting plate card, the amplifier that connects with data collecting plate card and connects with this amplifier;
Data processing equipment connects described data collector, handles the data from data collector;
The data result display device connects described data processing equipment, comprises the waveform output unit and the waveform location that connects with this waveform output unit, playback and the preservation unit that connect with described data processing equipment;
Data filtering device connects described data processing equipment;
The data storage and retrieval device connects described data filtering device, comprises data storage cell that connects with described data filtering device and data retrieval and the lead-out unit that connects with this data storage cell.
2. the nanochannel Single Molecule Detection system based on LabVIEW according to claim 1 is characterized in that described data collecting plate card is the PCI-6251 data collecting plate card.
3. the nanochannel Single Molecule Detection system based on LabVIEW according to claim 1 is characterized in that, described amplifier is a kind of in forward amplifier or the sign-changing amplifier.
4. the nanochannel Single Molecule Detection system based on LabVIEW according to claim 1 is characterized in that, described waveform output unit comprise time-the voltage pattern display module, time-the map of current display module and time-the resistance view display module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201696436U CN201852815U (en) | 2010-04-23 | 2010-04-23 | Lab VIEW-based nanometer passageway monomolecular detection system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010201696436U CN201852815U (en) | 2010-04-23 | 2010-04-23 | Lab VIEW-based nanometer passageway monomolecular detection system |
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| CN201852815U true CN201852815U (en) | 2011-06-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103246698A (en) * | 2013-03-29 | 2013-08-14 | 华东理工大学 | Method for processing data on basis of nanopore single-molecule detection system |
| CN112698085A (en) * | 2020-12-22 | 2021-04-23 | 南通大学 | Weak current detection device based on single-molecule electrochemical analysis and analysis method thereof |
-
2010
- 2010-04-23 CN CN2010201696436U patent/CN201852815U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103246698A (en) * | 2013-03-29 | 2013-08-14 | 华东理工大学 | Method for processing data on basis of nanopore single-molecule detection system |
| CN103246698B (en) * | 2013-03-29 | 2016-12-07 | 华东理工大学 | Data processing method based on nanometer passageway monomolecular detection system |
| CN112698085A (en) * | 2020-12-22 | 2021-04-23 | 南通大学 | Weak current detection device based on single-molecule electrochemical analysis and analysis method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110601 Termination date: 20150423 |
|
| EXPY | Termination of patent right or utility model |