CN201803942U

CN201803942U - Cuvette

Info

Publication number: CN201803942U
Application number: CN201020557175XU
Authority: CN
Inventors: 郑春阳; 魏国祥
Original assignee: TIANJIN QIANGWEITE BIO-TECH Co Ltd
Current assignee: TIANJIN QIANGWEITE BIO-TECH Co Ltd
Priority date: 2010-10-12
Filing date: 2010-10-12
Publication date: 2011-04-20
Anticipated expiration: 2020-10-12

Abstract

The utility model relates to a cuvette for detecting a micro sample. The cuvette comprises a sample charge channel arranged in the cuvette, a detection pool, a sample discharge channel, a sample recycling tube and a sealing rubber plug, wherein the detection pool has two parallel light-transmitting surfaces; the sample charge channel and the sample discharge channel which are connected with the detection pool are arranged above the detection pool; the sealing rubber plug is arranged at the inlet of the sample charge channel; and the sample recycling pipe is connected to the outlet of the sample discharge channel. Through the utility model, the micro sample can be measured accurately without loss, air bubbles can be prevented during sample charge, the sample recycling is facilitated and the operation is convenient.

Description

Cuvette

Technical field

The utility model relates to a kind of analysis cuvette, particularly a kind of cuvette that is used to detect micro-example.

Background technology

Development along with bioengineering and Protocols in Molecular Biology, current bioengineering industry has obtained development rapidly, about gene clone, plasmid is more and more frequent with the operation of relevant trace dnas such as genes of interest extraction and protein, detection for these μ g order of magnitude objects, the market general volume that detects of existing cuvette is at 0.5～3ml, reclaim the sample that obtains through gel and pass through volume dilution again greater than 0.5ml, object concentration can further reduce, so that the requirement that can not get effectively detection or do not reach the follow-up test high concentration after dilution detects because of object concentration is too low.Though the special cuvette of minimal sample volume 0.1ml is also arranged on the market, this micro-cuvette, need with specific cuvette support and necessarily corrective action could with the supporting use of spectrophotometer, it is more loaded down with trivial details to operate.

The utility model content

In view of this, the purpose of this utility model provides a kind of 20～500 μ l level sample volumes that are used for and detects the cuvette that requires, can satisfy the trace detection requirement, can also effectively prevent the generation of bubble in the application of sample process and detect after sample is effectively reclaimed.

Cuvette described in the utility model, form by the sample intake passage 1, detection cell 2, sample output passage 4, sample recovery tube 5 and the sealing plug 3 that are arranged in the cuvette, wherein detection cell 2 has two parallel transmitted light faces, material can be quartz or glass, above detection cell 2, be provided with the sample intake passage 1 and the sample output passage 4 that link to each other with detection cell 2, the porch of described sample intake passage 1 is provided with sealing plug 3, and the exit of mutually described sample output passage 4 is connected with sample recovery tube 5.

Further, the bottom of described sample intake passage 1 links to each other with the bottom of detection cell 2.

Further, described sample output passage 4 bottoms link to each other with the top of detection cell 2.

Further, described sample intake passage 1 porch links to each other with sealing plug 3, the plug form can be whole real cover the flexible glue plug or or with the supporting glue ring of syringe needle.

Further, described sample recovery tube 5 is threaded with sample output passage 4.

Preferably, described sample recovery tube 5 is an arc.

Preferably, the caliber of described sample intake passage 1 and sample output passage 4 is 0.22～2000 μ m.

In order to reach the requirement of trace detection, the utility model has improved the capacity in sample detection pond, the structure that has adopted sample intake passage, sample output passage and detection cell to link to each other, and forming one can be for the zone of small sample test, reach and accurately to measure, again the effect of not loss sample.Simultaneously the utility model has adopted the half-duplex channel sample introduction, and sample intake passage links to each other with at the bottom of the pond, and sample output passage links to each other with Chi Ding, and such design can reduce the gas dead angle, thus the generation of bubble when effectively having prevented application of sample.For the recovery of sample, the utility model has adopted one section arc sample recovery tube, can adopt the syringe exhaust to discharge from the sample recovery tube through the mode of sealing plug gas top sample then like this, has effectively solved the problem that sample reclaims, and is easy to operate.

Description of drawings

Accompanying drawing is used to provide further understanding of the present utility model, and constitutes the part of instructions, is used from explanation the utility model with embodiment one of the present utility model, does not constitute restriction of the present utility model.In the accompanying drawings:

Fig. 1 is the utility model cuvette structural representation;

Embodiment

As shown in Figure 1, cuvette described in the utility model, form by the sample intake passage 1, detection cell 2, sample output passage 4, sample recovery tube 5 and the sealing plug 3 that are arranged in the cuvette, wherein detection cell 2 has two parallel transmitted light faces, its shape can be tubulose, cube or cylindrical, and the area of two transparent surfaces can make and detect light just fully by being the best.Below the detection cell 2 with above be respectively equipped with sample intake passage 1 and the sample output passage 4 that links to each other with detection cell 2, wherein sample intake passage 1 passes through from the side of detection cell, but do not communicate with the detection cell side, and the bottom of sample intake passage 1 links to each other with the bottom of detection cell 2, can make sample directly enter the bottom of detection cell 2 like this, produce to prevent bubble; And the bottom of sample output passage 4 links to each other with the top of detection cell 2, the generation of bubble and the convenient recovery that detects the back sample liquid when this structure helps preventing application of sample; The caliber size of sample intake passage 1 and sample output passage 4 is 0.22～2000 μ m for caliber, and so effectively reduced volume is saved sample, and present embodiment is 1000 μ m; In addition, convenient for saving sample volume and application of sample, sample intake passage 1 and sample output passage 4 also can be variable-diameter structure, and sample injection port and discharging opening are taked wide footpath, and inner sample introduction and sample output passage are taked thin footpath.Sealing plug 3 is by inlaying the top that is fixed on sample intake passage 1, and purpose is to play the effect of sealing gas when sample introduction and recovery sample.The exit of sample output passage 4 is connected with sample recovery tube 5, and that connected mode can be selected is bonding, be threaded etc., and present embodiment is preferably and is threaded, and can play better fixation to sample recovery tube 5 like this, again can be for convenience detach.Sample recovery tube 5 is preferably the arc siphunculus, because certain camber is arranged, therefore can better reclaim the sample that detects after finishing.

The utility model cuvette adopts the micro syringe sampling in use, inserts then in the sealing plug, because the sealing plug has certain elasticity, can play good sealing function; Sample slowly is expelled in the sample intake passage through syringe, flows into the bottom of detection cell at last.When sample introduction was slow, available micro syringe exhaust adopted pneumatic jack liquid mode to quicken sample flow then.After detection cell is full of sample, can put into spectrophotometer and detect, after finishing, adopt syringe to take out full air once more and insert then and adopt pneumatic jack to impel sample to discharge by the sample recovery tube in the sealing plug, reclaim after arriving certain container through sample output passage.

The above is a preferred embodiment of the present utility model only, is not limited to the utility model, and for a person skilled in the art, the utility model can have various changes and variation.All within spirit of the present utility model and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.

Claims

1. cuvette, it is characterized in that: form by the sample intake passage (1), detection cell (2), sample output passage (4), sample recovery tube (5) and the sealing plug (3) that are arranged in the cuvette, wherein detection cell (2) has two parallel transmitted light faces, be provided with sample intake passage (1) and the sample output passage (4) that links to each other with detection cell (2) in detection cell (2) top, the porch of described sample intake passage (1) is provided with sealing plug (3), and the exit of described sample output passage (4) is connected with sample recovery tube (5).

2. cuvette according to claim 1 is characterized in that: the bottom of described sample intake passage (1) links to each other with the bottom of detection cell (2).

3. cuvette according to claim 1 is characterized in that: described sample output passage (4) bottom links to each other with the top of detection cell (2).

4. cuvette according to claim 1 is characterized in that: described sample recovery tube (5) is threaded with sample output passage (4).

5. according to claim 1 or 4 described cuvettes, it is characterized in that: described sample recovery tube (5) is an arc.

6. cuvette according to claim 1 is characterized in that: the caliber of described sample intake passage (1) and sample output passage (4) is 0.22～2000 μ m.