CN201765187U - Alga classifying and distinguishing instrument based on chlorophyll analysis - Google Patents
Alga classifying and distinguishing instrument based on chlorophyll analysis Download PDFInfo
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- CN201765187U CN201765187U CN2009202988871U CN200920298887U CN201765187U CN 201765187 U CN201765187 U CN 201765187U CN 2009202988871 U CN2009202988871 U CN 2009202988871U CN 200920298887 U CN200920298887 U CN 200920298887U CN 201765187 U CN201765187 U CN 201765187U
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Abstract
The utility model belongs to the technical field of environmental biological monitoring, specifically relating to an alga classifying and distinguishing instrument based on chlorophyll analysis. In the instrument, laser emitted by a mixed double-beam light source is irradiated on a sample detecting pool, and an exciting light color filter and an exciting light monochrome transilluminator are successively installed on a light path between the mixed double-beam light source and the sample detecting pool; and fluorescent light produced by exciting the sample in the sample detecting pool is projected onto a system analyzing unit through a secondary exciting light color filter, a secondary exciting light spectrum adjusting control unit and a light spectrum amplifier in sequence. The instrument can classify and distinguish algae quickly and conveniently according to excitation wavelength and light intensity variation as well as the wavelength and light intensity variation of secondary exciting light.
Description
Technical field
The utility model belongs to the environmental organism monitoring technical field, specifically relates to a kind of instrument of analyzing the algae classification and differentiating based on chlorophyll.
Background technology
The needs of the algae research that causes in view of the aggravation of state, inside and outside water body eutrophication degree with to body eutrophication, how to realize the analysis of interior algae kind of online, on the throne or real-time water body and quantity, and the eutrophication degree made judgement, more and more cause the attention of water quality monitoring department.What simultaneously, in research algal grown periodic process, also be necessary the content of total body algae and algae chlorophyll content to analyze the growth course and the period of different algal species, in conjunction with so that its growth tendency is made judgement.
At present, the method that detects algae in the water body both at home and abroad mainly adopts laboratory microscope detection method and chlorophyll detection technique.Wherein, the laboratory microscope monitoring method realizes the classification to algae in the water body by the way of morphological analysis and artificial counting, but is difficult to realize the labor of kind and quantity, therefore, when laboratory study algal grown and analyzing water body eutrophication degree, has very big limitation.Though the chlorophyll analytical technology can according to chlorophyll content what, total body density to algae is made judgement, but be difficult to dissimilar chlorophyll are analyzed, and, can not make classification to algae and water according to absorbing wavelength and the secondary excitation wavelength of algae in default of sufficient experimental data.
In a word, existing method all is unrealized based on the algae classification and the discriminating of chlorophyll difference analysis.
The utility model content
The purpose of this invention is to provide a kind of instrument of analyzing the algae classification and differentiating based on chlorophyll.
The technical solution adopted in the utility model is for achieving the above object:
A kind of instrument of analyzing the algae classification and differentiating based on chlorophyll, comprise and mix twin-beam light source 3, exciting light color filter 4, exciting light monochrome transmissive device 5, sample detection pond 6, secondary excitation light color filter 9, the adjusting of secondary excitation spectrum control module 10, spectrum projector 11 and systematic analysis unit 12, wherein mix twin-beam light source 3 emitted laser and be radiated on the sample detection pond 6, on the light path of mixing between twin-beam light source 3 and the sample detection pond 6, be provided with exciting light color filter 4 and exciting light monochrome transmissive device 5 successively; Samples are excited in the described sample detection pond 6, and the fluorescence that produces is regulated control module 10 by secondary excitation light color filter 9, secondary excitation spectrum successively and spectrum projector 11 is incident upon on the systematic analysis unit 12.
Described mixing twin-beam light source 3 is regulated control module 1 with the scanning of optics spectral line and is linked to each other, and the scanning of optics spectral line is regulated control module 1 by power supply 2 controls.6 upper ends, described sample detection pond are provided with sample floss hole 8, and the lower end is provided with continuous sample introduction mouth 9.Described sample floss hole 8 and continuous sample introduction mouth 7 are located at the homonymy in sample detection pond 6.Described mixing twin-beam light source for the tungsten lamp of emission 190nm～1100nm (+12V) and deuterium lamp (+130V, AC3V) the mixing twin-beam of Xing Chenging (ultraviolet/as seen) light source.
The advantage that the utility model had:
1. the utility model can be by mixing twin-beam light source (wavelength 190nm～1100nm) irradiation, and regulate the adjusting and the control of control module in conjunction with optics spectral line scanning, the chlorophyll characteristic absorption spectrum of different algal species in the combining target water body forms the excitation source at different algal species;
2. the excitation source that forms at different algal species of the utility model shines after the monitoring objective, different algal species can pass to reaction center by the absorption of light harvesting pigment complex (Light harvesting complex) and by it in the monitoring objective, chlorophyll is at molecule absorption luminous energy (absorption spectrum, be characteristic absorption spectrum) after, can be by stable ground state transition to unsettled excited state, the ground state so release energy again, wherein part energy discharges (secondary excitation) with the form of fluorescence, forms secondary excitation fluorescence;
3. the utility model is regulated control module in conjunction with spectrum and is carried out characteristic spectrum adjusting selection, formation feature secondary excitation fluorescence spectrum at the secondary excitation fluorescence of the energy formation of different algal species chlorophyll release;
4. the utility model can carry out the intensity amplification to the faint secondary excitation fluorescence that the release of chlorophyll energy forms, and clearly analyzes;
5. the utility model is analyzed different algal species in conjunction with making up main ALGAE CHARACTERISTICS absorption spectrum and secondary excitation spectra database in advance, by spectrum length and intensity size contained algal kind and quantity in the target water body is analyzed.
Description of drawings
To be the utility model analyze the algae classification and the instrumentation diagram differentiated (wherein the scanning of 1. optics spectral lines is regulated control module, 2. power supply control, 3. mixed twin-beam (ultraviolet/as seen) light source, 4. exciting light color filter, 5. exciting light monochrome transmissive device, 6. sample detection pond, 7. continuous sample introduction device, 8. sample discharging, 9. secondary excitation light color filter, 10. secondary excitation spectrum adjusting control module, 11. spectrum projectors, 12 systematic analysis unit) based on chlorophyll to Fig. 1.
Embodiment
Embodiment
Analyze the instrument (referring to Fig. 1) of algae classification and discriminating based on chlorophyll:
Comprise and mix twin-beam light source 3, exciting light color filter 4, exciting light monochrome transmissive device 5, sample detection pond 6, secondary excitation light color filter 9, the adjusting of secondary excitation spectrum control module 10, spectrum projector 11 and systematic analysis unit 12, mix twin-beam light source 3 and link to each other with optics spectral line scanning adjusting control module 1, the scanning of optics spectral line is regulated control module 1 by power supply 2 controls.Energized, mix twin-beam light source 3 emitted laser through exciting light color filter 4 after, pass and place the exciting light monochrome transmissive device 5 that mixes before the twin-beam light source 3, laser radiation is on sample detection pond 6; Samples are excited in the described sample detection pond 6, and the fluorescence that produces is regulated control module 10 by secondary excitation light color filter 9, secondary excitation spectrum successively and spectrum projector 11 is incident upon on the systematic analysis unit 12; Characteristic absorption wavelength, secondary excitation wavelength and light intensity that systematic analysis unit 12 is finished in the several seconds sample change, compare with the absorbing wavelength and the excitation wavelength of the built-in various different algal species in systematic analysis unit 12, finish kind and quantity that the different water bodys of on-line analysis contain algae.
6 upper ends, described sample detection pond are provided with sample floss hole 8, and the lower end is provided with continuous sample introduction mouth 9; Described sample floss hole 8 and continuous sample introduction mouth 7 are located at the homonymy in sample detection pond 6.Described mixing twin-beam light source for the tungsten lamp of emission 190nm～1100nm (+12V) and deuterium lamp (+130V, AC3V) the mixing twin-beam of Xing Chenging (ultraviolet/as seen) light source.
Claims (5)
1. instrument of analyzing the algae classification and differentiating based on chlorophyll, it is characterized in that: comprise and mix twin-beam light source (3), exciting light color filter (4), exciting light monochrome transmissive device (5), sample detection pond (6), secondary excitation light color filter (9), secondary excitation spectrum is regulated control module (10), spectrum projector (11) and systematic analysis unit (12), wherein mix twin-beam light source (3) emitted laser and be radiated on the sample detection pond (6), on the light path of mixing between twin-beam light source (3) and sample detection pond (6), be provided with exciting light color filter (4) and exciting light monochrome transmissive device (5) successively; The interior sample in described sample detection pond (6) fluorescence that produces that is excited is incident upon on the systematic analysis unit (12) by secondary excitation light color filter (9), secondary excitation spectrum adjusting control module (10) and spectrum projector (11) successively.
2. by the described instrument of analyzing the algae classification and differentiating based on chlorophyll of claim 1, it is characterized in that: described mixing twin-beam light source (3) is regulated control module (1) with the scanning of optics spectral line and is linked to each other, and the scanning of optics spectral line is regulated control module (1) by power supply (2) control.
3. by the described instrument of analyzing the algae classification and differentiating based on chlorophyll of claim 1, it is characterized in that: upper end, described sample detection pond (6) is provided with sample floss hole (8), and the lower end is provided with continuous sample introduction mouth (9).
4. by the described instrument of analyzing the algae classification and differentiating based on chlorophyll of claim 3, it is characterized in that: described sample floss hole (8) and continuous sample introduction mouth (7) are located at the homonymy of sample detection pond (6).
5. by the described instrument of analyzing the algae classification and differentiating based on chlorophyll of claim 1, it is characterized in that: described mixing twin-beam light source is the tungsten lamp of emission 190nm～1100nm and mixing twin-beam (3) light source that deuterium lamp forms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202988871U CN201765187U (en) | 2009-12-18 | 2009-12-18 | Alga classifying and distinguishing instrument based on chlorophyll analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202988871U CN201765187U (en) | 2009-12-18 | 2009-12-18 | Alga classifying and distinguishing instrument based on chlorophyll analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201765187U true CN201765187U (en) | 2011-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009202988871U Expired - Fee Related CN201765187U (en) | 2009-12-18 | 2009-12-18 | Alga classifying and distinguishing instrument based on chlorophyll analysis |
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| CN (1) | CN201765187U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013188952A1 (en) * | 2012-06-21 | 2013-12-27 | Honeywell Asca Inc. | Sensor for early detection of problems in algae cultures and related system and method |
| CN103562435A (en) * | 2011-05-25 | 2014-02-05 | 株式会社Crev | Light emission analyzing device |
-
2009
- 2009-12-18 CN CN2009202988871U patent/CN201765187U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103562435A (en) * | 2011-05-25 | 2014-02-05 | 株式会社Crev | Light emission analyzing device |
| WO2013188952A1 (en) * | 2012-06-21 | 2013-12-27 | Honeywell Asca Inc. | Sensor for early detection of problems in algae cultures and related system and method |
| US8848190B2 (en) | 2012-06-21 | 2014-09-30 | Honeywell Asca Inc. | Sensor for early detection of problems in algae cultures and related system and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: YANTAI INSTITUTE OF COASTAL ZONE RESEARCH, CHINESE Free format text: FORMER OWNER: YANTAI HAIANDAI SUSTAINABLE DEVELOPMENT INSTITUTE Effective date: 20120821 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20120821 Address after: Chunhui road Laishan District 264003 Shandong city of Yantai province No. 17 Patentee after: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Address before: Chunhui road Laishan District 264003 Shandong city of Yantai province No. 17 Patentee before: Yantai Haiandai Sustainable Development Institute |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110316 Termination date: 20131218 |