CN201737932U - Bioreactor - Google Patents
Bioreactor Download PDFInfo
- Publication number
- CN201737932U CN201737932U CN 201020295696 CN201020295696U CN201737932U CN 201737932 U CN201737932 U CN 201737932U CN 201020295696 CN201020295696 CN 201020295696 CN 201020295696 U CN201020295696 U CN 201020295696U CN 201737932 U CN201737932 U CN 201737932U
- Authority
- CN
- China
- Prior art keywords
- rotating shaft
- bio
- flange
- shell
- sparger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a bioreactor. A rotating shaft is inserted from the axis of a casing, the rotating shaft and the casing are sealed, the rotating shaft can rotate freely, the lower end of the rotating shaft is provided with a stirring paddle, the upper end of the rotating shaft is coaxially and fixedly provided with a distributor in the shape of a round platform, the upper part of the distributor is big, the lower part of the distributor is small, the upper end of the distributor is provided with a sprinkling device, a circulating pipeline is connected between the sprinkling device and the other end of the tank-shaped casing, the upper end of the tank-shaped casing is provided with an upper flange and an upper cover plate, the upper flange and the upper cover plate are fixed and are hermetically connected, the lower end of the tank-shaped casing is provided with a lower flange and a lower cover plate, the upper flange and the lower flange are connected by a plurality of connecting rods positioned at the edges, and the tank-shaped casing is tightly clamped and sealed. The utility model has the advantages of improving the structure of the bioreactor, increasing the contact area with oxygen greatly and enhancing the utilization rate of the oxygen. Meanwhile, because the bioreactor is arranged in a detachable type, the utility model can disassemble and assemble the bioreactor easily, is convenient to wash and also reduces the workload of disassembly and assembly.
Description
Technical field
The utility model relates to a kind of bio-reactor, is specifically related to a kind of bio-reactor that is used for virus vaccines and antibody producing.
Background technology
Existing companion's formula bio-reactor that stirs generally comprises airtight shell, rotating shaft and stirring rake, rotating shaft penetrates from the shell axle center, in the rotating shaft in the enclosure stirring rake is housed, this structure is in aspect ratio certain reaction device, contact surface is certain, therefore for the reactor of large volume, good biography oxygen, mass transfer be reached, rotating speed and air flow must be improved, but can produce bigger shearing force, cell growth inhibiting like this.Notification number be CN201437539U the real novel patent disclosure of China a kind of name be called " improved stirring type bioreactor ", the shell, rotating shaft and the stirring rake that comprise the closed tin shape, rotating shaft sealing penetrates from the shell axle center rotationally, in the rotating shaft in the enclosure stirring rake is housed, the device that increases the gas-to-liquid contact face is equipped with on top in described shell, is connected with liquid circulating apparatus between the bottom of the inlet of the device of this increase gas-to-liquid contact face and shell.The advantage of this utility model is: for the cell biological reactor of large volume, in diameter that does not need to increase stirring rake and speed of rotation and improve under the condition of air flow, utilize the recycle pump oar nutrient solution pump circulation of circulation line, liquid is the distributed flow mistake on sparger, increased contact area greatly with oxygen, thereby increased dissolved oxygen amount, improved oxygen utilization rate; The result of pump circulation has also strengthened the flowability of liquid, has obtained comparatively good mass transfer effect.Utilize this technology, can obtain the volume of culture bigger than pre-existing reactors.But this bio-reactor is because volume is bigger, and is Glass Containers, can bring very burden to stirring.
The utility model content
The utility model has overcome the deficiencies in the prior art, provides a kind of structure good, cleans biological reaction container easily, has solved problems of the prior art.
For solving above-mentioned technical problem, the utility model by the following technical solutions:
A kind of bio-reactor, comprise airtight jar shape shell, rotating shaft and stirring rake, rotating shaft is inserted from the axle center of shell, seal between rotating shaft and the shell, and rotating shaft can freely be rotated, the lower end of rotating shaft is equipped with stirring rake, the upper end of rotating shaft is coaxial to be installed with up big and down small truncated cone-shaped sparger, the sparger upper end is equipped with flusher, between the other end of flusher and jar shape shell, be connected with circulation line, described jar of shape shell upper end is provided with upper flange, upper cover plate, upper flange is fixed by bolt and upper cover plate and is tightly connected, described jar of shape shell lower end is provided with lower flange and lower cover, on, lower flange connects by a plurality of connecting rods that are positioned at the edge, and jar shape shell is clamped sealing.
Further technical scheme is:
Described sparger outside is a truncated cone-shaped, and its miner diameter end is connected with thrust-augmenting nozzle, fixedlys connected with rotating shaft in the top of thrust-augmenting nozzle.
Thrust-augmenting nozzle is passed in described rotating shaft, leaves the gap between the two.
Described flusher is a ring pipe, has a plurality of duck eyes that communicate with sparger on this ring pipe tube wall.
Described duck eye is distributed on the ring pipe tube wall.
Compared with prior art, the beneficial effects of the utility model are: the structure of having improved bio-reactor, increased greatly and the oxygen contact area, improve oxygen utilization rate, the utility model is because bio-reactor is set to removable simultaneously, can make things convenient for cleaning easily with its dismounting, also reduce the workload of dismounting.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is the flusher enlarged diagram.
Jar shape shell---1 rotating shaft---2 stirring rakes---3 sparger---4
Flusher---5 circulation lines---6 upper flanges---7 upper cover plate---8
Lower flange---9 lower covers---10 connecting rods---11 duck eye---12
Thrust-augmenting nozzle---13
Embodiment
Below in conjunction with accompanying drawing the utility model is further elaborated.
As shown in Figure 1, a kind of bio-reactor, comprise airtight jar shape shell 1, rotating shaft 2 and stirring rake 3, rotating shaft 2 is inserted from the axle center of jar shape shell 1, sealing between rotating shaft 2 and the shell 1, and rotating shaft 2 can freely rotate, and the lower end of rotating shaft 2 is equipped with stirring rake 3, the upper end of rotating shaft 2 is coaxial to be installed with up big and down small truncated cone-shaped sparger 4, and sparger 4 upper ends are equipped with flusher 5.Sparger 4 outsides are truncated cone-shaped, and its miner diameter end is connected with thrust-augmenting nozzle 13, fixedly connected with rotating shaft 2 in the top of thrust-augmenting nozzle 13, and thrust-augmenting nozzle 13 is passed in rotating shaft 2, leaves the gap between the two.As shown in Figure 2, flusher 5 is a ring pipe, has a plurality of duck eyes that communicate with sparger 12 on this ring pipe tube wall.Described duck eye 12 is distributed on the ring pipe tube wall.Between the other end of flusher 5 and jar shape shell 1, be connected with circulation line 6.The upper end of jar shape shell 1, be provided with upper flange 7, upper cover plate 8, upper flange 7 is fixed by bolt and upper cover plate 8 and is tightly connected, and described jar of shape shell 1 lower end is provided with lower flange 9 and lower cover 10, and lower flange 9 is fixed by bolt and lower cover 10 and is tightly connected.Upper and lower flange 7,9 connects by a plurality of connecting rods 11 that are positioned at the edge, and jar shape shell 1 is clamped sealing.
Claims (5)
1. bio-reactor, comprise airtight jar shape shell (1), rotating shaft (2) and stirring rake (3), rotating shaft (2) is inserted from the axle center of shell (1), sealing between rotating shaft (2) and the shell (1), and rotating shaft (2) can freely be rotated, the lower end of rotating shaft (2) is equipped with stirring rake (3), the upper end of rotating shaft (2) is coaxial to be installed with up big and down small truncated cone-shaped sparger (4), sparger (4) upper end is equipped with flusher (5), between the other end of flusher (5) and jar shape shell (1), be connected with circulation line (6), it is characterized in that: described jar of shape shell (1) upper end is provided with upper flange (7), upper cover plate (8), upper flange (7) is fixed by bolt and upper cover plate (8) and is tightly connected, described jar of shape shell (1) lower end is provided with lower flange (9) and lower cover (10), on, lower flange (7,9) connect by a plurality of connecting rods (11) that are positioned at the edge, and jar shape shell (1) is clamped sealing.
2. bio-reactor according to claim 1 is characterized in that: described sparger (4) is outside to be truncated cone-shaped, and its miner diameter end is connected with thrust-augmenting nozzle (13), fixedlys connected with rotating shaft (2) in the top of thrust-augmenting nozzle (13).
3. bio-reactor according to claim 1 is characterized in that: thrust-augmenting nozzle (13) is passed in described rotating shaft (2), leaves the gap between the two.
4. bio-reactor according to claim 1 is characterized in that: described flusher (5) is a ring pipe, has a plurality of duck eyes that communicate with sparger (12) on this ring pipe tube wall.
5. bio-reactor according to claim 4 is characterized in that: described duck eye (12) is distributed on the ring pipe tube wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020295696 CN201737932U (en) | 2010-08-18 | 2010-08-18 | Bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020295696 CN201737932U (en) | 2010-08-18 | 2010-08-18 | Bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201737932U true CN201737932U (en) | 2011-02-09 |
Family
ID=43553215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020295696 Expired - Fee Related CN201737932U (en) | 2010-08-18 | 2010-08-18 | Bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201737932U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690781A (en) * | 2012-04-28 | 2012-09-26 | 苏州金盟生物技术有限公司 | Method for replacing culture medium in perfusion-type cell culture |
-
2010
- 2010-08-18 CN CN 201020295696 patent/CN201737932U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690781A (en) * | 2012-04-28 | 2012-09-26 | 苏州金盟生物技术有限公司 | Method for replacing culture medium in perfusion-type cell culture |
| CN102690781B (en) * | 2012-04-28 | 2014-04-30 | 苏州金盟生物技术有限公司 | Method for replacing culture medium in perfusion-type cell culture |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110209 Termination date: 20150818 |
|
| EXPY | Termination of patent right or utility model |