CN201587947U - Standard molecule kit for detecting foreign genes of transgenic soya beans, corns, and paddy rice - Google Patents

Abstract

The utility model provides a standard molecule kit for detecting the foreign genes of transgenic soya beans, corns, and paddy rice. The standard molecule kit comprises a closed box 1 with a cover, wherein a sample carrying plate 2 and an Ep pipe 3 are mounted in the box, and pMD18-T-PAT-CP4-EPSPS-Cry1A (B)-BAR-RBCL plasmids are filled in the Ep pipe. The utility model can be used for qualitative detecting general positive standard molecules of CP4-EPSPS genes, Cry1A (B) genes, BAR genes, and PAT genes of different varieties of transgenic grain crops, has the advantages of lightness and handiness, and convenient and quick operation, and can not only solve the difficult problem that laboratories are generally lack of positive standard substances, but also improve detection efficiency and shorten detection period.

Description

Genetically engineered soybean, corn and paddy rice foreign gene detect uses the standard molecule test kit

(1) technical field

The utility model relates to the transgenosis detection technique, is specifically related to a kind of genetically engineered soybean, corn and paddy rice foreign gene detection standard molecule test kit.

(2) technical background

The PCR of transgenic product detects must be with a kind of accurate, reliable, stable reference material as positive control, to prevent false-negative appearance.Constructed in the world standard molecule all is that 1 goal gene is cloned in 1 plasmid at present, perhaps the internal control gene and the goal gene of a kind or strain are cloned in 1 plasmid simultaneously, when doing the detection of different plant species transgene component, need a plurality of standard molecules, more loaded down with trivial details.

(3) summary of the invention

The utility model purpose is to provide a kind of genetically engineered soybean, corn and paddy rice foreign gene that can be used for different varieties transgenosis food crop qualitative detection CP4-EPSPS gene, Cry1A (B) gene, BAR gene and pat gene to detect universal standard molecular agents box.

The purpose of this utility model is achieved in that it comprises an enclosure with cover, and load sample plate and an Ep pipe are housed in the enclosure, and pMD18-T-PAT-CP4-EPSPS-Cry1A (B)-BAR-RBCL plasmid is housed in the Ep pipe.

The utility model also has some technical characterictics like this:

1, described load sample plate is provided with the load sample hole, and the Ep pipe passes the load sample hole and is provided with.

The utility model is to utilize recombinant technology with genetically engineered soybean, main foreign gene Cry1A (B) gene of corn and paddy rice, the BAR gene, the CP4-EPSPS gene, pat gene and common confidential reference items RBCL gene all are cloned in the plasmid simultaneously, doing soybean, when corn and paddy rice transgene component qualitative detection, in positive control PCR reaction, add this standard molecule plasmid, internal standard gene RBCL gene and foreign gene CP4-EPSPS gene, Cry1A (B) gene, BAR gene and pat gene have all obtained amplification, and the amplified fragments size is consistent with the expection clip size, and only amplify the internal control gene fragment in the negative control, in the blank without any amplified fragments, show PCR reaction system works better, can judge whether contain the CP4-EPSPS gene the sample detection result, Cry1A (B) gene, BAR gene and pat gene.

Standard molecule in the utility model and other detect soybean, corn is different with the genetically modified standard molecule of paddy rice, other products needs at least 4 standard molecules promptly to need 4 EP pipes, and the utility model is with transgenosis food crop soybean common on the transgenosis market, main foreign gene Cry1A (B) gene of corn and paddy rice, the BAR gene, the CP4-EPSPS gene, pat gene and common confidential reference items RBCL gene all are cloned in the plasmid simultaneously, can be used for different varieties transgenosis food crop qualitative detection CP4-EPSPS gene, Cry1A (B) gene, the general positive standard molecule of BAR gene and pat gene.The utility model only needs promptly have only 1 EP pipe just to satisfy with 1 standard molecule and detects needs.Therefore, the utility model is lighter and handier, simple and efficient.Not only can solve the difficult problem that the laboratory generally lacks the positive criteria material, can also improve detection efficiency, shorten sense cycle.

(4) description of drawings

Fig. 1 is the utility model box body outside view;

Fig. 2 is the utility model load sample composition that hardens;

Fig. 3 is the transverse views of Fig. 2;

Fig. 4 is the utility model Ep pipe outside view.

(5) embodiment

Below in conjunction with the drawings and specific embodiments the utility model is further described:

In conjunction with Fig. 1-Fig. 4, present embodiment comprises a seal box with cover 1, and load sample plate 2 and an EP pipe 3 are housed in the box, in the Ep pipe 3 pMD18-T-PAT-CP4-EPSPS-Cry1A (B)-BAR-RBCL plasmid is housed, load sample hole 4 is arranged on 2 on the load sample plate, Ep pipe 3 passes load sample hole 4 and is provided with.

Claims (2)

1. a genetically engineered soybean, corn and paddy rice foreign gene detect and use the standard molecule test kit, and it comprises an enclosure with cover, it is characterized in that being equipped with in the described enclosure load sample plate and an Ep pipe.

2. genetically engineered soybean according to claim 1, corn and paddy rice foreign gene detect and use the standard molecule test kit, it is characterized in that described load sample plate (2) is provided with load sample hole (4), and Ep pipe (3) passes load sample hole (4) and is provided with.

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