CN201533832U - Simple dual-purpose thermal and mechanical pain threshold detector - Google Patents
Simple dual-purpose thermal and mechanical pain threshold detector Download PDFInfo
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- CN201533832U CN201533832U CN2009201549720U CN200920154972U CN201533832U CN 201533832 U CN201533832 U CN 201533832U CN 2009201549720 U CN2009201549720 U CN 2009201549720U CN 200920154972 U CN200920154972 U CN 200920154972U CN 201533832 U CN201533832 U CN 201533832U
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Abstract
A simple dual-purpose thermal and mechanical pain threshold detector comprises a main bracket, an animal testing platform, a thermal radiation stimulator, a mechanical Von Frey stimulator, an operation controller and an enclosed box for accommodating animals. By combining the thermal radiation stimulator and the mechanical stimulator on the basis of thermal and mechanical pain threshold detection principles, the utility model can realize pain behavior experiments induced by two kinds of stimulus without changing a testing environment, so as to maximally reduce the influence of environmental factors on the results of animal behavior experiments; as the two stimulators can be separated from each other, and the stimulus spatial positions can be adjusted within a certain range, direct measurement on receptive fields apart from rat paws can be realized, that is, the animal measurement range is enlarged; and compared with the traditional pain threshold detector, the utility model has the advantages of simple and reasonable design, convenient operation, stable performance, low cost and the like, and can be popularized and applied in ordinary medical colleges and universities as well as colleges of life science.
Description
Affiliated technical field
This utility model belongs to neuroscience field animal behavior experimental apparatus, is specifically related to the dual-purpose pain tester of animal heat radiation and mechanical stimulus.
Background technology
During periphery inflammation or europathology pain, corresponding limbs of rat or body receptive field generation hyperpathia (hyperalgesia) and touch and bring out pain (allodynia), showing stimulates and machinery touches the sensitivity enhancing of stimulation heat radiation.Therefore, it is noxious stimulation that the radiant heat that the normal employing of experiment infrared emanation bulb (greater than electric filament lamp or the Halogen light of 40W) scioptics converge and the von Frey nylon fibril of different size (length and diameter) touch the crooked standardization masterpiece that produces, and brings out the escape reaction of shrinking back of rat limbs or body.Animal is under clear-headed rest state, body surface receptive field with nocuity heat radiation and machinery (touching) stimulation in rats inflammation district or pathological changes innervation district, the record heat radiation begins to animal its limbs or body to be left the time of thermal source as hot withdrawal reflelx (Thermal Withdraw Latency incubation period, TWL), unit is second (s); With the von Frey fibril minimum sandards masterpiece that can bring out the animal withdrawal reflelx be machinery touch bring out the withdrawal reflex threshold value (Mechanical Withdraw Latency, MWL), unit be the gram (g).Therefore, TWL and the MWL Chang Zuowei objective quantification index pain nature and extent of zoologizeing.
Before the experiment beginning, to animal continuous measurement 2-3 days, measured 3-5 every day, and each 5-10 minute at interval, former 3 days meansigma methods also promptly contrasted as the basic threshold of pain.After animal modeling (inflammation pain or europathology pain model etc.) or the pharmaceutical intervention, in corresponding point in time measurement pain, each point in time measurement 3-5 time, each 5-10 minute at interval.For eliminating the influence of animal individual difference, also compare (contralateral side) and measure with strong side (with respect to Ipsilateral ipsilateral side) body or limbs, and record data.
Present thermal stimulus dolorimeter and machinery (touch or press) stimulate dolorimeter, comprising: hot plate stimulates dolorimeter, Mus tail radiostimulation dolorimeter, Mus pawl radiostimulation dolorimeter, Mus cleoid thorn to stimulate dolorimeter, biped counterbalanced procedure dolorimeter, paw to stimulate dolorimeter or the like.Though these instruments can both objectively respond the pain behavior of animal, but its common limitation is arranged all: (1) can only be measured as the periphery receptive field with animal vola or afterbody, the pain research of peripheral nervous system is limited in down the spinal nerves sensory system of waist section, and the nervi trigeminus sensory system of domination women's head-ornaments portion and the breast waist section spinal nerves sensory system of breast waist and belly can't be carried out; (2) being confined to single stimulation measures.After animal is finished heat radiation survey pain, need to change experimental apparatus and carry out the machinery survey bitterly, animal itself, local environment changes, and needs certain hour to adapt to new environment again, and behavioral experiment process and result all influential (3) are cost an arm and a leg, complex structure makes to promote to be restricted.
Summary of the invention
Technical problem to be solved in the utility model is to overcome the little and limitation such as cost an arm and a leg of single, the tested animal receptive field of the existing survey pain apparatus function scope of application, provides a kind of and first heat and mechanical stimulus two class pain sensation measuring devices is combined, enlarges animal pain measurement receptive field, simple in structure, stable performance, little power consumption, the mechanical dual-purpose dolorimeter of simple and easy heat with low cost.
The technical scheme that its technical problem that solves this utility model adopts is: it comprises that dolorimeter main body rack, detection platform, heat radiation stimulator, mechanical von Frey fiber stimulator, animal are placed and encloses case, Electromechanical Control box.
Main body rack of the present utility model is: the rectangular base that stainless steel material is made and the pillar of four adjustment heights constitute " four footstools " shape structure, and outward appearance is porcelain black.Rectangular base is long to be 50.0cm, and wide is 32.0cm, and height is 3.0cm.Heat radiation and mechanical stimulus device place on the base and can move freely.Pillar is respectively 3.0cm and 3.2cm by diameter, long mutually nested being made of steel drum that is 20cm, and its outside indicates graduation mark, can realize altitude mixture control by the screw in degree of tightness pillar centre position, and altitude range is 20cm to 40cm.The pillar tip designs becomes towards the fan-shaped unfilled corner in intermediary right angle, in order to fixing support animal testing platform.
Detection platform of the present utility model is: comprise the thick printing opacity of 3mm, hot organic plastics web plate and web plate holder frame, be positioned at directly over the hot mechanical stimulus device, by four pillar institutes of main body rack support.The organic plastics web plate is long to be 38cm, and wide is 22.5cm, and the circular hole that distributes symmetrically on the web plate, Circularhole diameter are 1.5mm, is 5mm with horizontal adjacent circular holes distance vertically.Circular hole allows the von Frey fiber of mechanical stimulus device freely to pass through.The holder frame of support web plate is by the wide 1cm of being, long is 41.0cm, and wide is 23.5cm, the rectangular symmetrical hollow structure that height surrounds for wide of the organic plastics of 1.5cm, and the center is the holder bar of a wide 1cm, in order to the support web plate.
Heat radiation stimulator of the present utility model is: the heat radiation device of being made up of electric filament lamp DS, optically focused heat pipe, collecting lens reaches by digital display time relay (AC220V, 50/60Hz, 0～99.9s) J, contact button AN1 with lock, AN2, alternation commutator transformer (IN AC220V, 50/60Hz; OUT 8～12V, electromechanical controlling device two parts that 20～50W) T, on and off switch K connect to form constitute.
(1) intussusception formed inside and outside the optically focused heat pipe of heat radiation device was respectively two steel drums of 2.5cm and 2.8cm by diameter, and intussusception outside pars intermedia is fixed with screw that can free degree of tightness, and optically focused heat pipe outside is coated with thermal sleeve in case the experimenter is scalded.The electric filament lamp of 12V/50W and lamp socket thereof are fixed on the interior pipe upper end of optically focused heat pipe, and two power lines pass with electromechanical controlling device from optically focused heat pipe bottom and link to each other.The collecting lens that diameter (d) and focal length (f) are respectively 2.5cm and 3.0cm is horizontally fixed on optically focused heat pipe outer tube upper end.Make electric filament lamp be in focal length of lens position by regulating the intussusception drum length, thereby make photo-thermal converging action the best.Cylindrical heat radiation device is sleeved on the elongated cylindrical stepping tooth bar of mechanical stimulus device by the loop overlapping shape metal structure of dicyclo parallel connection, by the parbuckle screw both orientation in adjustable heat radiostimulation source, also can freely take off separation, realize that with this dual-purpose function of surveying the pain device of heat machinery integrates and separate.
(2) electromechanical controlling device is: the electric filament lamp DS that is fixed on the heat-resisting lamp socket of optically focused fluorescent tube is connected with output lead (white) O1 of alternation commutator transformer T by power line, and another power line line is connected with the wiring point of contact button AN1 with lock (redness).Another output lead (white) O2 of alternation commutator transformer T then is connected to the offside wiring point of this contact button AN1 with lock, is connected with No. 10 wiring points of relay J again, and button AN1 intermediate connection point then is connected with No. 11 wiring points of relay J.Two input line (redness) IN1, the IN2 of alternation commutator transformer T are connected to No. 1 and No. 2 wiring points of relay respectively, again respectively with adjacent 4 wiring points of power control switch K in be diagonal bits 2 wiring points link to each other, 2 wiring points that are diagonal bits then link to each other with two power lines of attaching plug in addition.About contact button AN2 with lock (green) two wiring points be connected to respectively No. 9 of relay J with No. 10 wiring sites.
Carrying out heat radiation stimulates when bringing out hyperpathia (hyperalgesia) experiment, and the line that plugs in turns on the power switch, at first regulate the time set of maximum timing on the time digital display relay, usually be set to 300, promptly the time upper limit was made as 30.0 seconds, relay automatic disconnection after 30 seconds.This is provided with purpose is to prevent that thermal light source from excessively shining the animal vola and causing and burn.The heat radiation stimulator is aimed at middle part, animal vola,, control the stepper tooth bar bidirectional-movement switch of mechanical stimulating apparatus and adjust stimulator in suitable height according to stimulator front end marker post.Press earlier SR, again by startup/pause button, this moment, heat radiation stimulated beginning, and the time relay picks up counting simultaneously, and observes illuminated animal behavior, the escape behavior of the pawl reaction if occur contracting, by startup/pause button, relay stops timing, shows certain time numerical value once more, the experimenter notes this numerical value, finishes heat radiation test for the first time.After 5-10 minute, press SR at interval, the numerical value reset-to-zero that shows on the time digital display relay by startup/pause button, repeats previous experimentation again.Every animal is tested 3-5 time every day, gets its meansigma methods as the heat radiation of animal pawl TWL incubation period that contracts.
Von Frey fibre machinery stimulator of the present utility model is: by surveying the mechanical von Frey stimulating apparatus that different dynamics specification nylon fibers, fiber holder, loop overlapping shape metal structure form and electromechanical controlling device two parts formation of being made up of stepper (band tooth bar 6W reducing motor), speed regulator SC and tooth bar bidirectional-movement switch DK.
(1) mechanical von Frey stimulating apparatus is: the length of side that organic plastics is made is on 1.5cm, the fiber holder of height for the pentahedron shape of 2cm, each fixes 1 von Frey fiber of having surveyed different dynamics to each face by boring and with adhesion agent, and this fiber is made by the nylon wire of different-diameter and length.Summarized results sees the following form one after the length of fiber, diameter and the Precision Electronics Balance dynamometry.Fiber is installed on the fiber holder and numbering by the dynamics increasing order in the direction of the clock, and it is a dynamometry group that per 5 fibers are fixed on the holder, totally 4 dynamometry groups.Long be 8cm, it is chimeric that wide height is quarter butt shape handle and the holder intermediate vertical of 0.5cm, can freely take off or load onto holder with the replacing fiber of standardization power on the same group not; Also can rotate the handle that links to each other with holder, in same dynamometry group fiber, change different dynamics fibers.The fiber holder passes the loop overlapping shape metal structure and is fixed on by a screw on the circular bar shape tooth bar of step device, can be around the tooth bar level or adjust up and down in its orientation.The loop overlapping shape metal structure that also there are two rings the lower end and connect in the tooth bar is fixedly set on the optically focused heat pipe of tooth bar and heat radiation stimulator by screw respectively, reaches with this machinery and heat radiation stimulator are incorporated on the device.
Table one. self-control von Frey each diameter length of fiber and corresponding standardization power gather
Sequence number diameter (mm) length (mm) standardization power (g)
1 0.18 40 0.15
2 0.18 35 0.24
3 0.18 30 0.38
4 0.25 40 0.42
5 0.25 35 0.48
6 0.25 30 0.64
7 0.30 40 1.60
8 0.30 35 2.10
9 0.30 30 2.45
10 0.38 40 2.35
11 0.38 35 2.70
12 0.38 30 3.60
13 0.45 40 3.80
14 0.45 35 5.30
15 0.45 30 6.20
16 0.50 40 8.60
17 0.50 35 18.50
18 0.50 30 21.40
19 0.55 40 12.60
20 0.55 30 24.00
(2) electromechanical controller is: step device for band tooth bar LD 2-61K-R exchange decelerator (220V, 6W, 50Hz, 90r/min), the long 500mm of tooth bar par, range 400mm, linear reciprocation speed 1mm/s, maximum use thrust 196N.Step device is connected with the connecting line of speed regulator SC by the six conductor cable line that sends, the reciprocating two-way switch DK of control tooth bar is connected with speed regulator SC, be that two-way switch DK control wiring point upwards is connected with CW wiring place of speed regulator SC, the intermediate connection point that control disconnects is connected with COM wiring place of speed regulator SC, controls downward lower end wiring point and is connected with CCW wiring place of speed regulator SC.At last, power cord plug is connected with two AC wiring points of speed regulator SC.
Carry out mechanical stimulus and touch when bringing out pain (allodynia) experiment, the line that plugs in, the speed regulator display lamp is bright, opens the speed regulator on and off switch, and the speed regulator knob is threaded to the centre position, gradually speed is transferred to suitable size in the experimentation again; In addition, handle by rotation von Frey fiber holder in the experiment makes the dynamics of von Frey fiber use successively by order from small to large, and with the web plate circular hole place at place, von Frey fiber alignment animal vola, beat stepper tooth bar bidirectional-movement switch to the Up position this moment, tooth bar at the uniform velocity rises, von Frey fiber passes circular hole and touches the animal vola and bend, beat to the Down position with being about to the bidirectional-movement switch, tooth bar at the uniform velocity descends, von Frey fiber leaves the animal receptive field, and then the one-time mechanical stimulation is finished.If having, animal do not lift the sufficient lower limb reaction of contracting, then rotary handle increases progressively the von Frey fiber that uses great efforts in turn, the lower limb reaction of contracting the first time occurs until animal, the experimenter writes down the standardization power of von Frey fiber correspondence this moment, and mechanical stimulus is for the first time touched and brought out the pain EOT.After 5-10 minute, use von Frey fiber successively at interval, repeat a preceding experimentation by dynamics order from small to large.Every animal is tested 3-5 time every day, averages as the mechanical stimulus of animal pawl MWL incubation period that contracts.
Operation control of the present utility model is: belonged to the milky cuboid standard Electromechanical Control chamber of making by aluminium alloy, the length and width height is respectively 28cm, 26cm, 12cm, experimenter's operating area of front panel, be distributed with digital display screen, contact button AN1 with lock, AN2, power control switch K, tooth bar bidirectional-movement gauge tap DK, the speed regulator SC of digital display time relay J successively according to design in advance, all there is corresponding English mark each components and parts below; Dynamo-electric box rear board has power line to pass from the below wire hole.
Animal placement of the present utility model is enclosed case and is: the no bottom surface that organic plastics glass is made and the hollow cuboid structure of end face, enclose the long 38cm of case, wide 20cm, high 15cm, 3 black baffle plates of center fixed will enclose case and be divided into four microscler spaces such as big independence such as grade, allow once to put into an animal, and case is enclosed in each test can put into four tested animal (rat) simultaneously, enclosing the box top has two case lids with holes, flees from test zone to stop animal.
The beneficial effects of the utility model are, by heat and machinery are surveyed the scientific combination of device bitterly, tentatively be implemented in not change and carry out the pain behavior reaction that two kinds of stimulations are brought out under the animal testing environment, reduce the influence of environmental factors to greatest extent the animal behavior experimental result.In addition,, can do certain locus to stimulus and set, enlarge the receptive field that animal is measured by mechanical separation.
Description of drawings
Below in conjunction with drawings and Examples this utility model is further specified.
Fig. 1 is whole structure figure of the present utility model.
Fig. 2 is the main body rack of dolorimeter.
Fig. 3 is the detection platform of dolorimeter.
Fig. 4 is 1 a partial enlarged drawing among Fig. 3.
Fig. 5 is the heat radiation stimulator of dolorimeter.
Fig. 6 is 1 a vertical section structural map among Fig. 5.
Fig. 7 is the mechanical Von Frey stimulator of dolorimeter.
Fig. 8 is 3,4 a partial enlarged drawing among Fig. 7.
Fig. 9 is the front view of Fig. 8.
Figure 10 is the operation control of dolorimeter.
Figure 11 is that case is enclosed in the animal placement of dolorimeter.
Figure 12 is heat radiation stimulator electromechanical controlling device relay circuit figure
Figure 13 is the dynamo-electric connection line figure of heat radiation stimulator
Figure 14 is speed regulator and two-way gauge tap circuit connection diagram
1. dolorimeter main body racks among the figure, 2. detection platform, 3. heat radiation stimulator, 4. mechanical stimulus device, 5. operation control, 6. animal is placed and encloses case, 7. telescopic prop, 8. base, 9. organic plastics web plate, 10. holder frame, 11. optically focused heat pipes, 12. photo-thermal collecting lenses, 13. incandescent bulb, 14. guidance panels, 15. control knobs, 16. power line, 17. steppers, 18. speed regulators, 19. self-control von Frey fiber, 20.von Frey fiber holder, 21. governor switches, 22. the holder handle, 23. time digital display relays, 24. startup/pause buttons, 25. SR, 26. relay power switches, 27. tooth bar bidirectional-movement gauge taps, 28. the reducing motor speed regulator, 29. heat radiation stimulator attaching plugs, 30. mechanical stimulus device attaching plugs, 31. the dynamo-electric box of controller, 32. casing, 33. case lids, 34. baffle plates.
The specific embodiment
Below in conjunction with drawings and Examples this utility model is further described, but this utility model is not limited to these embodiment.
In Fig. 1, the heat radiation stimulator of integration and von Frey fibre machinery stimulator are positioned in the middle of the main body rack base, by move freely stimulator on base, can make stimulus (heat radiation light source and von Frey fiber) contain whole detection platform substantially.Height Adjustable platform by regulating telescopic prop makes stimulus can arrive tested animal receptive field smoothly in suitable position.Operating different controllers can control stimulator separately respectively and carry out the different pain performance testings of bringing out.
In the embodiment shown in fig. 1, the heat radiation stimulator of integration and von Frey mechanical stimulus device are connected in the controller case by power line separately, and draw attaching plug separately.Controller front panel left field is heat radiation stimulator control zone, comprises that time figure shows indicator panel (0-99.9s), red green different colours contact button with lock (controlling timing respectively starts (time-out) and reset) and power control switch.The front panel right side area is the mechanical stimulus control zone, comprises speed regulator (containing speed regulator on and off switch and speed adjusting knob) and tooth bar bidirectional-movement gauge tap.
The heat radiation stimulator is made up of electric filament lamp (12V/50W), optically focused heat pipe, collecting lens, regulates electric filament lamp and lens distance to adjust the spot diameter (being generally 2.0mm) that light source converges by telescopic adjustment optically focused heat pipe.During use, mobile stimulator makes hot spot aim at tested animal receptive field (being generally the center, vola) on base, behind the power connection, press startup/pause button on the control panel (redness), electric filament lamp is luminous, and the instant heating radiostimulation begins, time digital display relay picks up counting, observe the limbs of illuminated animal simultaneously,, press startup/pause button once more if escape reaction (as the lower limb that contracts) appears in animal, electric filament lamp goes out, the instant heating radiostimulation stops, and time digital display relay stops timing and shows numerical value sometime, and the experimenter writes down numerical value, then the end of pain behavior reaction is brought out in thermostimulation this time, behind the certain hour (5-10min), repeat said process more at interval, generally repeat 3-5 time, with the incubation period of meansigma methods reaction animal escape reaction, incubation period is long, and then animal pain threshold height is insensitive to pain; Shorten incubation period, illustrates that then the animal pain threshold reduces, and is in hyperpathia (hyperalgesia) state.
Von Frey fibre machinery stimulator constitutes by surveying different dynamics specification nylon fibers, fiber holder, loop overlapping shape metal clamper and adjustable speed step device (band tooth bar 6W reducing motor).A fiber holder then constitutes by being fixed with 5 different dynamics nylon fiber organic plastics medicated caps and organic plastics bar.During use, by dynamics from small to large use nylon fiber (can use the nylon fiber of 5 different dynamics by rotary plastic bar on clamper successively) smoothly successively, the stimulator on the movable base makes the most advanced and sophisticated tested animal receptive field (being generally the center, vola) of aiming at of nylon fiber.Behind the power connection, open the speed regulator on and off switch, the speed governing knob is located at shelves at a slow speed, tooth bar bidirectional-movement gauge tap is upwards pressed, the nylon fiber that then is fixed on the tooth bar begins at the uniform velocity to rise, regulate the speed regulator knob to suitable movement velocity, major part was crooked after nylon fiber was run into the animal receptive field.Use the nylon fiber of minimum dynamics at first, change by dynamics incremental order rotational fixation device bar successively then and select nylon fiber, when (lower limb contracts) reaction appears escaping in animal, tooth bar bidirectional-movement gauge tap is pressed extremely to lower gear, nylon fiber moves downward, after leaving animal receptive field certain distance (2-3cm), tooth bar bidirectional-movement gauge tap is pressed to middle off-state the nylon fiber stop motion.The standardization power of nylon fiber is represented the dynamics of animals received mechanical stimulus impression at this moment.Dynamics is big more, and then animal is brought out the pain threshold height of pain to mechanical stimulus, and is insensitive to pain; Dynamics is more little, illustrates that the pain threshold of animal is low, touches and brings out pain (alloydia) obviously.
Claims (1)
1. dual-purpose dolorimeter of simple and easy machinery, comprising that main body rack, animal testing platform, heat radiation stimulator, mechanical Von frey stimulator, operation control and animal are placed encloses case, it is characterized in that:
(1) said main body rack is: stainless steel material is made " four footstools " shape structure of the pillar formation of rectangular base and four adjustment heights, outward appearance is porcelain black, heat radiation stimulator and mechanical stimulus device place on the base and can move freely, four pillar outsides indicate graduation mark, can realize altitude mixture control by screw in the middle of the degree of tightness, altitude range is 20cm to 40cm, and the capital end is designed to towards the fan-shaped unfilled corner in intermediary right angle, in order to fixing support animal testing platform;
(2) said animal testing platform is: the holder frame of the thick printing opacity of 3mm, hot organic plastics rectangular otter board and below support web plate, circular hole symmetrically distributes on the organic plastics web plate, Circularhole diameter is 1.5mm in the plate, be 5mm vertically with horizontal adjacent circular holes distance, circular hole allows the von Frey fiber of mechanical stimulus device freely to pass through, the holder frame of support web plate is the rectangular symmetrical hollow structure that is surrounded for wide of the organic plastics of 1cm by wide, the center is the support web plate holder bar of wide 1cm, and the detection platform top is the animal placement and encloses case;
(3) said heat radiation stimulator is: by 12V/50W electric filament lamp DS, the optically focused heat pipe, the heat radiation device that collecting lens is formed reaches by digital display time relay J, contact button AN1 with lock, AN2, alternation commutator transformer T, on and off switch K connects to form electromechanical controlling device two parts and constitutes, the inside and outside intussusception of two steel drums of the optically focused heat pipe of heat radiation device forms, intussusception outside pars intermedia is fixed with screw that can free degree of tightness, optically focused heat pipe outside is coated with thermal sleeve in case the experimenter is scalded, the electric filament lamp of 12V/50W is fixed on the interior pipe upper end of optically focused heat pipe together with lamp socket, two power lines pass with electromechanical controlling device from optically focused heat pipe bottom and link to each other, collecting lens is horizontally fixed on optically focused heat pipe outer tube upper end, can make electric filament lamp be in focal length of lens position by regulating the intussusception drum length, thereby make convergence of rays effect the best, cylindrical heat radiation device is sleeved on the elongated cylindrical stepping tooth bar of mechanical stimulus device by the loop overlapping shape metal structure of dicyclo parallel connection, by parbuckle screw both adjustable heat radiostimulation device orientation, also can freely take off separation, realize that with this dual-purpose function of surveying the pain device of heat machinery integrates and separate, electromechanical controlling device is: the electric filament lamp DS that is fixed on the heat-resisting lamp socket of optically focused fluorescent tube is connected by the output lead O1 of power line with an alternation commutator transformer T, another root lead is connected with a contact button AN1 wiring point with lock, another output lead O2 of alternation commutator transformer then is connected to the offside wiring point of this contact button AN1 with lock, and then be connected with No. 10 wiring sites of relay J, button intermediate connection point then is connected with No. 11 wiring points of relay J, two input lines of alternation commutator transformer are connected to No. 1 and No. 2 wiring points of relay respectively, respectively again with 4 wiring points of power control switch K in be diagonal bits two wiring points link to each other, in addition two wiring points that are diagonal bits then link to each other with two power lines of attaching plug, and two wiring points are connected to No. 9 and No. 10 wiring sites of relay J respectively about another contact button AN2 with lock;
(4) said mechanical von Frey stimulator is: by surveying different dynamics specification nylon fibers, the fiber holder, the mechanical von Frey stimulating apparatus that the loop overlapping shape metal structure is formed reaches by band tooth bar 6W reducing motor stepper GH, electromechanical controlling device two parts that speed regulator SC and tooth bar bidirectional-movement switch DK form constitute, machinery von Frey stimulating apparatus is: on the fiber holder of 5 bodily forms that organic plastics is made, each fixes 1 von Frey fiber of having surveyed different dynamics to each face by boring and with adhesion agent, this fiber is made by the nylon wire of different-diameter and length, fiber is installed on the fiber holder and numbering by the dynamics increasing order in the direction of the clock, it is a dynamometry group that per 5 fibers are fixed on the holder, totally 4 dynamometry groups, quarter butt shape handle and holder intermediate vertical are chimeric, can freely take off or load onto holder to change the not fiber of standardization power on the same group; Also can be by rotating the handle that links to each other with holder, in same group of fiber, change different dynamics fibers, the fiber holder passes the loop overlapping shape metal structure and is fixed on the circular bar shape tooth bar upper end of step device by a screw, can be around the tooth bar level or adjusts up and down in its orientation, the loop overlapping shape metal structure that also there are two rings the lower end and connect in the tooth bar, be fixedly set on the optically focused heat pipe of tooth bar and heat radiation stimulator by screw respectively, reach with this machinery and heat radiation stimulator are incorporated on the device, electromechanical controller is: step device is connected with the connecting line of speed regulator SC by the six conductor cable line that sends, the reciprocating two-way switch DK of control tooth bar is connected with junction point on the speed regulator, be that two-way switch control wiring point upwards is connected with CW wiring place of speed regulator, the intermediate connection point that control disconnects is connected with COM wiring place of speed regulator, controls downward lower end wiring point and is connected with CCW wiring place of speed regulator; At last, power cord plug is connected with two AC wiring points of speed regulator;
(5) said operation control by: constituted by aforesaid heat radiation electromechanical controlling device, mechanical von Frey electromechanical controlling device and standard Electromechanical Control case, milky cuboid standard Electromechanical Control chamber, its front panel is experimenter's operating area, the digital display screen of the digital display time relay J that distributes successively, contact button AN1 with lock, AN2, power control switch K, tooth bar bidirectional-movement gauge tap DK, speed regulator SC; Rear board has power line to pass from line hole, below;
(6) said animal is placed and encloses case and be: the hollow cuboid structure that does not contain end face and bottom surface that colourless organic plastics is made, enclosing has 3 black baffle plates will enclose case in the middle of the case to be divided into four independently identical big or small cuboid spaces, make that enclosing case allows once to put into four rats to be measured, enclosing the box top has two identical size case lids with holes, flees from test zone to stop animal.
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| CN2009201549720U CN201533832U (en) | 2009-05-19 | 2009-05-19 | Simple dual-purpose thermal and mechanical pain threshold detector |
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| CN2009201549720U CN201533832U (en) | 2009-05-19 | 2009-05-19 | Simple dual-purpose thermal and mechanical pain threshold detector |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095614B (en) * | 2014-07-10 | 2017-01-25 | 中国人民解放军第四军医大学 | Rat balance based multifunctional pain threshold measuring apparatus and method |
| CN107049236A (en) * | 2016-12-16 | 2017-08-18 | 北京大学 | A kind of In The Rat Sole threshold of pain measuring system |
| CN109528162A (en) * | 2018-11-12 | 2019-03-29 | 江汉大学 | A kind of toy pain-measuring equipment and its application |
| CN109700438A (en) * | 2019-02-27 | 2019-05-03 | 复旦大学 | A kind of compound somatosensory stimulator for giving experimental animal mechanical force and high temperature |
| CN110897609A (en) * | 2019-11-13 | 2020-03-24 | 袁红斌 | Pain sense testing device for combined stimulation |
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2009
- 2009-05-19 CN CN2009201549720U patent/CN201533832U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095614B (en) * | 2014-07-10 | 2017-01-25 | 中国人民解放军第四军医大学 | Rat balance based multifunctional pain threshold measuring apparatus and method |
| CN107049236A (en) * | 2016-12-16 | 2017-08-18 | 北京大学 | A kind of In The Rat Sole threshold of pain measuring system |
| CN109528162A (en) * | 2018-11-12 | 2019-03-29 | 江汉大学 | A kind of toy pain-measuring equipment and its application |
| CN109700438A (en) * | 2019-02-27 | 2019-05-03 | 复旦大学 | A kind of compound somatosensory stimulator for giving experimental animal mechanical force and high temperature |
| CN110897609A (en) * | 2019-11-13 | 2020-03-24 | 袁红斌 | Pain sense testing device for combined stimulation |
| CN110897609B (en) * | 2019-11-13 | 2023-10-03 | 上海长征医院 | Pain sense testing device with combined stimulation |
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