CN201512535U - Blade PCR fast sampling device - Google Patents
Blade PCR fast sampling device Download PDFInfo
- Publication number
- CN201512535U CN201512535U CN2009201119614U CN200920111961U CN201512535U CN 201512535 U CN201512535 U CN 201512535U CN 2009201119614 U CN2009201119614 U CN 2009201119614U CN 200920111961 U CN200920111961 U CN 200920111961U CN 201512535 U CN201512535 U CN 201512535U
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- pcr
- plunger
- blade
- syringe
- needle
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Abstract
A blade PCR fast sampling device comprises a syringe and needle heads, wherein a solid needle head is fixed at the top end of a plunger; the point of the solid needle head is positioned in a hollow needle head but does not exceed the end part of the hollow needle head; a spring is arranged between the plunger and the top part of a needle cylinder; and two pins penetrating the edges of the needle cylinder are arranged at the rear part of the piston of the plunger and are in no contact with the plunger. The device is used for fast and conveniently sampling template DNA samples required by PCR amplification, therefore, the number of blades of PCR template DNA materials required during sampling is small, blade samples to be sampled have the same size, the PCR of the samples can be directly carried out, and the PCR effect and the repeatability are good.
Description
Technical field
The utility model relates to a kind of blade PCR rapid sampling attachment, prepares the required template DNA sample of round pcr fast, belongs to biological technical field.
Background technology
Along with developing rapidly of Protocols in Molecular Biology, round pcr has been widely used in the every field of genetic breeding, as seed purity identify, species specificity is identified, the detection of tracking, the assignment of genes gene mapping, genetic analysis and the transfer-gen plant of the analysis of sibship, target gene etc.
The first step of PCR-based technology must be extracted the genomic dna of testing sample as template, carries out corresponding pcr amplification.DNA extraction method commonly used has CTAB extraction method and SDS extraction method.The DNA quality that CTAB (cetyltriethylammonium bromide) method and SDS (sodium laurylsulfonate) method are extracted is higher and amount is big, but the required cost of these two kinds of DNA extraction methods is higher, technical strong, the blade consumption is big, program complex work amount is big, when the breeding of utilization molecular marker assisted selection, often need a large amount of colonies to be measured, waste time and energy cost greatly so seem.About how can prepare template DNA more fast, on different crops, successively report some, handle spire directly as pcr template method, TE grinding and succusion, shearing method as SDS single stage method, microwave water-boiling method, NaOH about the quick extractive method of DNA.Though these methods have been simplified the program of template DNA preparation to a certain extent, improved the efficient of preparation, but still had weak point, the operation efficiency of these methods is not high enough, or the amplification of the template DNA of preparation is undesirable, usually can not amplify the purpose fragment.
At present, when preparing pcr template DNA sample fast, also there is not a kind of quick and easy sampling unit.The method of the preparation of simplifying template DNA to a certain extent of priority report, these methods are in the difference of when sampling sample thief amount in order to reduce, need carry out weighing to each sample, or the consistent leaf sample of naked-eye observation clip size, these methods are all more time-consuming, also are difficult to overcome the difference on the sample volume.
Summary of the invention
The utility model has overcome the deficiency time-consuming, bothersome, the sample volume difference in size of taking a sample in the existing pcr template of the preparation fast DNA sampling process, filled up the blank of no rapid sampling attachment, provide a kind of can be quick, convenient, the blade PCR sampling unit of sampling amount unanimity.
The utility model comprises syringe and two parts of syringe needle, fixes a solid syringe needle 3 on plunger 1 top 2, and the tip of solid syringe needle 3 places in the hollow needle 4, and the tip of solid syringe needle 3 does not exceed the end of hollow needle 4; Between the top of the top 2 of plunger 1 and syringe 5, a spring 6 is arranged; At 2 rear portions, top of plunger 1 two pins 7 that run through the syringe edge are arranged, the pin 7 that runs through the syringe edge does not contact with plunger 1.
The beneficial effects of the utility model are a kind of blade pcr template DNA sampling units, the required template DNA sample of sampling pcr amplification that can be quick and easy; The reagent chemicals that in sampling process, does not need other auxiliary plant and instrument and traditional extraction DNA; The blade amount of required pcr template DNA material is few when being used to take a sample, and is fit to the material that blade is few, blade is little; The leaf sample size of using blade pcr template DNA sampling unit to be taken a sample is consistent, does not worry the different detected materials DNA concentration difference problem that what cause owing to sample; The leaf sample that blade pcr template DNA sampling unit is taken a sample can directly carry out PCR, has saved other treatment steps, and its PCR effect and good reproducibility, the detection of suitable especially a large amount of colonies.
Description of drawings
Fig. 1: be the structural representation of the utility model blade PCR rapid sampling attachment.
1. plungers among the figure, 2. plunger top, 3. solid syringe needle, 4. hollow needle, 5. syringe, 6. spring, 7. pin.
Embodiment
The utility model comprises syringe and two parts of syringe needle.Fix a solid syringe needle 3 on plunger 1 top 2, the tip of solid syringe needle 3 places in the hollow needle 4, and the tip of solid syringe needle 3 does not exceed the end of hollow needle 4; Between the top of the top 2 of plunger 1 and syringe 5, a spring 6 is arranged; At 2 rear portions, top of plunger 1 two pins 7 that run through the syringe edge are arranged, the pin 7 that runs through the syringe edge does not contact with plunger 1.
The utility model is taken a sample fast and is used operation steps as follows:
1, prepares pcr template DNA material blade.
2, material blade is put on the clean filter paper, blade PCR rapid sampling attachment is vertical with blade, stabs the blade of drawing materials gently with the hollow needle end of device.
3, material blade stays the material sample of hollow needle diameter in the end of hollow needle.
4, the hollow needle that will have a blade PCR rapid sampling attachment of material sample stretches into the bottom of PCR pipe, touches plunger, makes the material sample of staying the hollow needle end be released by solid syringe needle, and sample is put in the bottom of PCR pipe.
Claims (1)
1. blade PCR rapid sampling attachment, comprise two parts of syringe and syringe needle, it is characterized in that: fix a solid syringe needle (3) on plunger (1) top (2), the tip of solid syringe needle (3) places in the hollow needle (4), and the tip of solid syringe needle (3) does not exceed the end of hollow needle (4); Between the top of the top (2) of plunger (1) and syringe (5), a spring (6) is arranged; At the rear portion, top (2) of plunger (1) two pins (7) that run through the syringe edge are arranged, the pin (7) that runs through the syringe edge does not contact with plunger (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201119614U CN201512535U (en) | 2009-09-18 | 2009-09-18 | Blade PCR fast sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201119614U CN201512535U (en) | 2009-09-18 | 2009-09-18 | Blade PCR fast sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201512535U true CN201512535U (en) | 2010-06-23 |
Family
ID=42483942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009201119614U Expired - Fee Related CN201512535U (en) | 2009-09-18 | 2009-09-18 | Blade PCR fast sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201512535U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111647504A (en) * | 2020-06-10 | 2020-09-11 | 成都嘉予科技有限公司 | Quick PCR reaction tube and instrument thereof |
-
2009
- 2009-09-18 CN CN2009201119614U patent/CN201512535U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111647504A (en) * | 2020-06-10 | 2020-09-11 | 成都嘉予科技有限公司 | Quick PCR reaction tube and instrument thereof |
| CN111647504B (en) * | 2020-06-10 | 2023-07-04 | 赵毅 | Quick PCR reaction tube and instrument thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100623 Termination date: 20160918 |