CN201434821Y - Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer - Google Patents
Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer Download PDFInfo
- Publication number
- CN201434821Y CN201434821Y CN2009201396138U CN200920139613U CN201434821Y CN 201434821 Y CN201434821 Y CN 201434821Y CN 2009201396138 U CN2009201396138 U CN 2009201396138U CN 200920139613 U CN200920139613 U CN 200920139613U CN 201434821 Y CN201434821 Y CN 201434821Y
- Authority
- CN
- China
- Prior art keywords
- sample cell
- ultraviolet
- fluorophotometer
- sample pool
- spot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
An ultraviolet-visible absorption accessory of a Turner 10-AU field fluorophotometer relates to a fluorophotometer, in particular to an ultraviolet-visible absorption accessory which is applicable toa Turner 10-AU field fluorophotometer. An ultraviolet-visible absorption accessory of a Turner 10-AU field fluorophotometer which can absorb a detecting accessory and can remain the original fluorescence detecting function is provided, and comprises a sample pool, a sample pool bracket and an L-shaped draw bar, wherein the sample pool is provided with an incident surface, an exit surface and a reflecting surface, the incident surface is perpendicular to the exit surface, and reflecting surface is in an angle of 45 degrees with incident lights, a reflecting mirror is embedded on the reflectingsurface, the incident surface, the exit surface and the reflecting surface of the sample pool are made from quartz, and the other part of the sample pool is made of common glass, the sample pool is embedded on a clamping groove of the sample pool bracket, and the vertical bar of the L-shaped draw bar is arranged on the center groove of the sample pool bracket, and the transverse bar of the L-shaped draw bar is arranged on the lower portion of the sample pool.
Description
Technical field
The utility model relates to a kind of fluorophotometer, particularly a kind of ultraviolet-visible absorption measurement annex that is mainly used in the on-the-spot fluorophotometer of Tener 10-AU.
Background technology
In Marine Sciences, environmental science, commercial production, because source, the complex structure of sample, the factor that influences its variation is numerous, is difficult for storage and transport, and the on-the-spot fluorescence and the demand of absorption measurement are more and more urgent.
10-AU on-the-spot fluorophotometric instrument in Tener is a kind of portable field fluorescent instrument that U.S. Tener company produces, and can carry out continuous flow and detect and independent sample analysis.Its range of application comprises compositions such as ocean research, chlorophyll analysis, lake and reservoir management, fluorescent tracing, crude oil leakage detection.Its structure mainly is made up of light source, optical filter, sample chamber, detecting device, data handling system five parts.Since be provided with waterproof case and waterproof filter set, built-in data collecting instrument, but the automatic range setting has superpower stability, makes it can satisfy the requirement of in-situs tester device.Therefore if on the basis that keeps its original on-the-spot fluorometric assay function, develop its absorption measurement function, to realizing target compound extinction spectrum and fluorescence spectrum on-line determination, comparison simultaneously, the variation of form in physical environment of reflection target compound is significant.
On an instrument, realize to absorb and existing report of work that fluorescence is measured simultaneously, more commonly with two independent detection systems or functional module is integrated in the same system.CTD as liquid chromatograph ultraviolet spectrometry/fluorescence detector or configuration absorption and fluorescent probe.
The applicant provides a kind of fluorospectrophotometer ultraviolet-visible absorption appendix in the patent of invention of notification number for CN1155077, comprise pond frame and scattering cell body, the pond frame by scattering cell body frame link to each other side by side with cell rack form, the side establishes the through hole that is on the photofluorometer light path, the scattering cell body is established the scatterer in pond body and the pond body, the pond body places scattering cell body frame, and the inwall of the outside of pond body and pond frame coincide.Utilize the scattering nature of material and the synchronous scanning function of photofluorometer on the commodity photofluorometer, to carry out the ultraviolet-visible spectrophotometric spectra analysis of material, with extremely low cost, make fluorospectrophotometer possess fluorescence analysis and two kinds of functions of ultraviolet-visible absorption analysis, reach the purpose of dual-use.Can't realize on-site measurement but exist, or cost an arm and a leg, or be difficult for being applied to deficiencies such as lake and inshore water.
Summary of the invention
The purpose of this utility model is to provide a kind of absorption measurement annex while also to keep the ultraviolet-visible absorption appendix of the on-the-spot fluorophotometer of Tener 10-AU of original fluorometric assay function.
The utility model is provided with sample cell, sample cell frame and L type pull bar, sample cell is provided with the plane of incidence, exit facet and reflecting surface, the plane of incidence is vertical with exit facet, reflecting surface and incident light are 45, embed catoptron on the reflecting surface, sample cell is embedded on the draw-in groove of sample cell frame, and the montant of L type pull bar embeds and is located in the sample cell frame central recess, and the cross bar of L type pull bar is located at the sample cell below.
Described sample cell frame is preferably black organic plastics sample cell frame.
The plane of incidence of described sample cell is the quartzy plane of incidence, and exit facet is quartzy exit facet, and reflecting surface is quartzy reflecting surface, and the other parts of sample cell are that simple glass is made.
During use,, select the optical filter and the attenuator of suitable wavelength scope at first according to determination object.The utility model is put into the camera bellows of fluorophotometer, fix, put on the self-control specimen holder that is fixed in the sample chamber after again the sample of required mensuration being changed over to sample cell, cover the sample hatchcover, can measure with screw.
Utilization configuration fluorophotometer of the present utility model can be on the basis that keeps its on-the-spot fluorescence detection function, on-line determination when realizing highly sensitive absorption spectrum of measurand and fluorescence spectrum.When the utility model was used for the mensuration of potassium dichromate and potassium permanganate solution, sensitivity was than high about 1 times and 0.5 times respectively of Cary 100 ultraviolet-visible pectrophotometer.
When measuring when the utility model is applied to the absorption of humic acid aqueous solution and fluorescence, its measurement result is satisfied.The migration Study on Transformation of region response in the research of coloured dissolved organic matters in the coastal zone waters (CDOM) and dissolved organic matter (DOM) and the whole world change, the regional marine eco-environment and ocean organic contaminant is in close relations.CDOM has extinction and fluorescent characteristic simultaneously, thereby absorption photometry and fluorescent spectrometry become the main research means of this research field in succession.But the source of CDOM, DOM, complex structure, the factor that influences its variation in environment is numerous, is difficult for storage and transport.Therefore have the development of the field instrumentation of measurement function simultaneously of absorption and fluorescence, to realizing CDOM extinction spectrum and fluorescence spectrum on-line determination, comparison simultaneously, reflection CDOM variation of form in physical environment is significant.
The on-the-spot luminoscope of 10-AU after the improvement is successfully realized the mensuration of absorption spectrum under the situation of not damaging former instrument, gained sensitivity is than Cary 100 ultraviolet-visible pectrophotometer height, and detection limit and precision also reach the requirement of assay determination.The utility model is easy and simple to handle, cheap, be easy to dismounting, and configurable different optics suite, thereby overcomes the shortcoming of original fitting spectrum continuity deficiency, has to promote and commercialization value.The realization that absorption and fluorescence scene are measured simultaneously, for the migration conversion process of work place study DOM, CDOM and organic pollutant and be subjected to the variation of its form under the biological or chemical factor affecting and interaction that strong instrument is provided, for a new method basis has been established in the migration conversion of the biomass geochemistry circulation of research source of students key element and pollutant.
The utility model utilizes the direct reflection of light to change light path to be implemented in the ultraviolet-visible absorption measurement that carries out liquid sample on the on-the-spot fluorophotometer of Tener 10-AU.For the convenience that does not change the original accessory structure of instrument and use, the utility model has designed the detachable absorption measurement annex that can be used for on-the-spot sample according to the design feature of fluorophotometer sample chamber camera bellows.
In sum, the utlity model has following outstanding feature:
A. former instrument is not done any change, can be used for the absorption measurement of on-the-spot sample;
B. easy and simple to handle, cheap, be easy to the dismounting;
Measure when c. having realized on-the-spot absorption of sample and fluorescence, this research to Marine Sciences, environmental science has great significance;
Thereby d. configurable different optics suite overcomes the shortcoming of original fitting spectrum continuity deficiency.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment.
Fig. 2 is the structural representation of sample cell frame.
Fig. 3 is the left TV structure synoptic diagram of Fig. 2.
Fig. 4 is the plan structure synoptic diagram of Fig. 2.
Fig. 5 is the structural representation of sample cell.
Fig. 6 is the structural representation of L type pull bar.
Fig. 7 is the on-the-spot luminoscope light path of a former 10-AU synoptic diagram.
Fig. 8 is the on-the-spot luminoscope light path of the 10-AU synoptic diagram behind configuration the utility model.
Fig. 9 measures the standard working curve of potassium dichromate aqueous solution for on-the-spot luminoscope of configuration 10-AU of the present utility model and Cary 100 ultraviolet-visible pectrophotometers.In Fig. 9, horizontal ordinate is concentration C (mg/L), and ordinate is an absorbance; Curve i measures potassium dichromate, y=0.0204x+0.0059 R for the on-the-spot luminoscope of configuration 10-AU of the present utility model
2=0.9925; Curve ii is that Cary 100 measures potassium dichromate, y=0.0103x+0.0166 R
2=0.9981.
Figure 10 measures the standard working curve of potassium permanganate solution for on-the-spot luminoscope of configuration 10-AU of the present utility model and Cary 100 ultraviolet-visible pectrophotometers.In Figure 10, horizontal ordinate is concentration C (mg/L), and ordinate is an absorbance; Curve iii measures potassium permanganate, y=0.009x+0.0121 R for the on-the-spot luminoscope of configuration 10-AU of the present utility model
2=0.9960; Curve iv is that Cary 100 measures potassium permanganate, y=0.0059x+0.0204 R
2=0.9984.
Figure 11 is the ultraviolet-visible absorption spectroscopy (Cary 100) of potassium dichromate and potassium permanganate.In Figure 11, horizontal ordinate is wavelength (nm), and ordinate is an absorbance A; Solid line is potassium dichromate (20.0mg/L), and dotted line is potassium permanganate (32.0mg/L).
Figure 12 measures the result of HA fluorescence and absorption for the on-the-spot luminoscope of configuration 10-AU of the present utility model.In Figure 12, horizontal ordinate is concentration C (mg/L), and left ordinate is an absorbance A, and right ordinate is fluorescence intensity F; Curve a is for measuring HA fluorescence, y=3.24x+2.33, R
2=0.9945; Curve b absorbs y=0.0333x+0.0117 R for measuring HA
2=0.9981.
Embodiment
The utility model is described in further detail below in conjunction with specific embodiments and the drawings.
Shown in Fig. 1～6, the utility model is provided with sample cell 2, the movable L type of sample cell frame 1 and metal pull bar 3, sample cell 2 is provided with the plane of incidence, exit facet and reflecting surface, the plane of incidence is vertical with exit facet, reflecting surface and incident light are 45, embed catoptron 4 on the reflecting surface, the plane of incidence of sample cell 2, exit facet and reflecting surface are that quartz material is made, the other parts of sample cell 2 are that simple glass is made, sample cell 2 is embedded on the draw-in groove 11 of sample cell frame 1, the montant 31 of L type pull bar 3 embeds to be located in sample cell frame 1 central recess 12, and the cross bar 32 of L type pull bar 3 is located at sample cell 2 belows.
Described sample cell frame is a black organic plastics sample cell frame.
Fig. 7 provides the on-the-spot luminoscope light path of former 10-AU synoptic diagram.Light by light source 71 sends behind exciter filter 72, obtains the exciting light of required wavelength.If its intensity is I
0, after sample cell 73, a part of light is absorbed by sample solution, so its intensity in transmission is kept to I.After wherein fluorescent material is excited, will to around emitting fluorescence.When measuring fluorescence, the light on 90 ° of directions through emission optical filter 74 after, most parasitic lights are eliminated, and obtain needed fluorescence signal F, fluorescence signal F delivers to photomultiplier detector 75 and detects, testing result is shown by registering instrument 76.
Fig. 8 provides the on-the-spot luminoscope light path of the 10-AU synoptic diagram behind configuration the utility model.Light by light source 71 sends behind exciter filter 72, obtains the exciting light of required wavelength, and the transmitted intensity of going into by attenuator 81 is I
0, and enter the measuring light road, and then measure the absorption in the light path by catoptron 84 reflections that the corner of sample cell 82 embeds, obtain needed fluorescence signal and deliver to photomultiplier detector 75 and detect, testing result is shown by registering instrument 76.Because most of solution transmitted intensity I+F are more a lot of by force than fluorescence intensity F,, prevent the generation of photobleaching so need the voltage of control luminous flux or reduction photomultiplier.On the light path except that adding attenuator 81, increase attenuator 83 (but multi-disc) again and the photomultiplier transit tube voltage transferred to I and reach this purpose.When carrying out fluorometric assay, only need specimen holder is taken off, change former fluorometric assay specimen holder and optics suite and get final product through attenuator 83.
In order to investigate performance of the present utility model, under identical instrument condition, use Cary 100 ultraviolet-visible pectrophotometers and the utility model respectively.
Measure the potassium dichromate of serial variable concentrations and the absorbance of potassium permanganate solution with the on-the-spot luminoscope of 10-AU and Cary 100 ultraviolet-visible pectrophotometers (standard configuration liquid pool) of configuration absorption measurement external member respectively, and difference drawing curve, the result as shown in Figures 9 and 10, the correlation coefficient r of gained working curve all reaches more than 0.9962, show that the absorption of detected solution in the whole absorption measurement scope and the relation between its concentration meet langbobier law, i.e. the change of light path is influence not.The designed absorption measurement external member of experiment confirm is feasible.
The on-the-spot luminoscope of 10-AU after the improvement is measured the sensitivity of potassium dichromate and potassium permanganate solution respectively than high about 1 times and 0.5 times of Cary 100 ultraviolet-visible pectrophotometers (standard configuration liquid pool).Its reason can be explained by result shown in Figure 11.General spectrophotometer is that (as the variation of 360 ± 5nm) luminous fluxes as quantitative basis, and the on-the-spot luminoscope of the 10-AU after improving is (as the variation of 310～390nm) luminous fluxes as quantitatively basic with wide wavelength coverage with certain slit width.Though because the restriction of experiment process technology, the goodness of fit between self-control sample cell and the sample cell frame is not enough, make sample cell position instability in light path, the reappearance of testing result is not as commercial apparatus, but satisfied the requirement that correlation analysis is measured, only need to improve the processing technology of sample cell, then can obtain better result.
Soil ulmin (HA) is the main constituent of CDOM, and existing extinction characteristic is had fluorescent characteristic again, and the mutual relationship of its absorption characteristic and fluorescent characteristic is the focus that present people pay close attention to.So measure when employing HA aqueous solution checks the on-the-spot luminoscope of 10-AU after the improvement to be used for extinction and fluorescent characteristics.Prepare the HA aqueous solution of a series of variable concentrations after purified, survey its absorbance with homemade absorption measurement external member respectively, survey its fluorescent value with instrument first wife CDOM fluorometric assay external member.The results are shown in Figure 12.The result shows, measures when the on-the-spot luminoscope of the 10-AU after the improvement can be realized absorption of HA aqueous solution and fluorescence.10-AU itself is exactly a field instrumentation, and improved instrument promptly has on-the-spot absorption measurement function, adds that external member changes simple and easyly, can realize absorbing in the same water body in the certain hour span and the mensuration of fluorescence at research ship or field condition.
The acquisition of The above results not only has important significance for theories, has more realistic meaning simultaneously.This absorption external member is easy and simple to handle, cheap, be easy to dismounting, thereby and configurable different optics suite overcome the shortcoming of original fitting spectrum continuity deficiency, have and promote and commercialization value.The realization that absorption and fluorescence scene are measured simultaneously, for the migration conversion process of work place study DOM, CDOM and organic pollutant and be subjected to the variation of its form under the biological or chemical factor affecting and interaction that strong instrument is provided, for a new method basis has been established in the migration conversion of the biomass geochemistry circulation of research source of students key element and pollutant.
Claims (3)
1. the ultraviolet-visible absorption appendix of the on-the-spot fluorophotometer of Tener 10-AU, it is characterized in that being provided with sample cell, sample cell frame and L type pull bar, sample cell is provided with the plane of incidence, exit facet and reflecting surface, the plane of incidence is vertical with exit facet, reflecting surface and incident light are 45, embed catoptron on the reflecting surface, and sample cell is embedded on the draw-in groove of sample cell frame, the montant of L type pull bar embeds and is located in the sample cell frame central recess, and the cross bar of L type pull bar is located at the sample cell below.
2. the ultraviolet-visible absorption appendix of the on-the-spot fluorophotometer of Tener 10-AU as claimed in claim 1 is characterized in that described sample cell frame is a black organic plastics sample cell frame.
3. the ultraviolet-visible absorption appendix of the on-the-spot fluorophotometer of Tener 10-AU as claimed in claim 1, the plane of incidence that it is characterized in that described sample cell is the quartzy plane of incidence, and exit facet is quartzy exit facet, and reflecting surface is quartzy reflecting surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201396138U CN201434821Y (en) | 2009-07-24 | 2009-07-24 | Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201396138U CN201434821Y (en) | 2009-07-24 | 2009-07-24 | Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201434821Y true CN201434821Y (en) | 2010-03-31 |
Family
ID=42053634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009201396138U Expired - Fee Related CN201434821Y (en) | 2009-07-24 | 2009-07-24 | Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201434821Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103412105A (en) * | 2013-08-26 | 2013-11-27 | 武汉大学 | Underway measuring device for three water color constituents |
| CN109772490A (en) * | 2019-01-22 | 2019-05-21 | 福建师范大学 | A kind of specimen holder for sample detection |
| CN113670868A (en) * | 2021-07-16 | 2021-11-19 | 天津大学 | CDOM processing method of on-site ultraviolet fluorescence sensor |
-
2009
- 2009-07-24 CN CN2009201396138U patent/CN201434821Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103412105A (en) * | 2013-08-26 | 2013-11-27 | 武汉大学 | Underway measuring device for three water color constituents |
| CN103412105B (en) * | 2013-08-26 | 2015-01-14 | 武汉大学 | Underway measuring device for three water color constituents |
| CN109772490A (en) * | 2019-01-22 | 2019-05-21 | 福建师范大学 | A kind of specimen holder for sample detection |
| CN113670868A (en) * | 2021-07-16 | 2021-11-19 | 天津大学 | CDOM processing method of on-site ultraviolet fluorescence sensor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100545631C (en) | Multifunctional light absorption, scattering and emission spectrometer based on surface plasma-wave | |
| Wu et al. | Stand-off detection of chemicals by UV Raman spectroscopy | |
| JP4933271B2 (en) | Handheld device with a disposable element for chemical analysis of multiple specimens | |
| Zeng et al. | Development of in situ sensors for chlorophyll concentration measurement | |
| Clarke et al. | Developments in marine pCO2 measurement technology; towards sustained in situ observations | |
| Zhang et al. | Toward sensitive determination of ammonium in field: A novel fluorescent probe, 4, 5-dimethoxyphthalaldehyde along with a hand-held portable laser diode fluorometer | |
| CN102928390A (en) | On-line detection method and device for chlorophyll concentration in water body based on two detectors | |
| CN202196011U (en) | Portable multifunctional analyzer | |
| CN201434821Y (en) | Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer | |
| Chen et al. | Detection of water quality parameters in Hangzhou Bay using a portable laser fluorometer | |
| Harfmann et al. | Composition and lability of photochemically released dissolved organic matter from resuspended estuarine sediments | |
| CN201819876U (en) | Liquid phase sample detection tank for ultraviolet absorption and fluorescence spectra | |
| Zhan et al. | Seasonal variation of water-soluble brown carbon in Qingdao, China: Impacts from marine and terrestrial emissions | |
| CN102288566A (en) | Portable multifunctional analyzer and testing method thereof | |
| CN214844782U (en) | Optical path-variable multi-parameter water quality monitoring device based on spectrum method | |
| CN202869980U (en) | Portable oil spill online detector | |
| Hou et al. | Oil-spill detection sensor using ultraviolet-induced fluorescence for routine surveillance in coastal environments | |
| CN210037588U (en) | Absorption spectrum test system | |
| CN201788146U (en) | Pulsed xenon lamp type soil nutrient tester | |
| CN116337793A (en) | In-situ online measurement device and method for sea water turbidity and CDOM content | |
| Kang et al. | An ultra-high sensitivity fluorescence sensor based on fluorescence technology | |
| CN206906241U (en) | A kind of double light source nutrient salt analyzer | |
| CN112147101A (en) | Portable water quality analyzer and method for soluble organic matters and nitrate nitrogen | |
| Radiul et al. | Variation of Stokes Shift and Peak Wavelength Shift as a Sensing Probe for Detection of Lead in Water Using Laser Induced Fluorescence Resonance Energy Transfer | |
| CN214703332U (en) | Spectrum/fluorescence integrated detector for measuring ammonia nitrogen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100331 Termination date: 20120724 |