CN109772490A - A kind of specimen holder for sample detection - Google Patents
A kind of specimen holder for sample detection Download PDFInfo
- Publication number
- CN109772490A CN109772490A CN201910059288.2A CN201910059288A CN109772490A CN 109772490 A CN109772490 A CN 109772490A CN 201910059288 A CN201910059288 A CN 201910059288A CN 109772490 A CN109772490 A CN 109772490A
- Authority
- CN
- China
- Prior art keywords
- sample
- sample cell
- photomultiplier tube
- sliding rail
- beam splitter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000001514 detection method Methods 0.000 title claims abstract description 32
- 239000000523 sample Substances 0.000 claims abstract description 155
- 238000012360 testing method Methods 0.000 claims abstract description 23
- 239000012488 sample solution Substances 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000005855 radiation Effects 0.000 claims abstract description 7
- 238000002835 absorbance Methods 0.000 claims description 5
- 230000003447 ipsilateral effect Effects 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000003287 optical effect Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 3
- 239000012088 reference solution Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000005622 photoelectricity Effects 0.000 description 2
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Abstract
The present invention relates to a kind of specimen holders for sample detection, first sample cell is for containing reference liquid, second sample cell is for containing testing sample solution, beam splitter is set on the line of ultraviolet source and the first sample cell, the beam splitter is obliquely installed, reflecting mirror is identical as the setting direction of beam splitter, the reflecting mirror is set on the radiation direction that ultraviolet light reflects on beam splitter, second sample cell is set on the radiation direction reflected on reflecting mirror, the position of first photomultiplier tube is corresponding with the position of the first sample cell, the position of second photomultiplier tube is corresponding with the position of the second sample cell.The specimen holder structure for being used for sample detection is simple, the detection of reference liquid and testing sample solution can be carried out simultaneously, it avoids constantly substituting the indoor sample of sample, reduce the influence of human factor, improve the stability of device, experimental period is reduced, the efficiency of experiment is improved, ensure that the accuracy and reliability of experimental result.
Description
Technical field
The present invention relates to laboratory inspection apparatus field more particularly to a kind of specimen holders for sample detection.
Background technique
UV-VIS spectrophotometry is to carry out a kind of qualitative, common hand of structural analysis and quantitative analysis to substance
Section.In the prior art, survey of the UV-VIS spectrophotometry to solution concentration to be measured is generally realized with ultraviolet specrophotometer
Fixed, concrete operations are detected respectively to reference solution and testing sample solution using ultraviolet specrophotometer, then right
The numerical value of detection is compared the solubility for finally obtaining testing sample solution.
Plug-type specimen holder is set in the sample room of ultraviolet specrophotometer, generally by manual form push with
Extraction, during push-and-pull, sample overfill is easily overflowed in specimen holder, causes the error of measuring result, and utilize ultraviolet
There is inefficiency than relatively time-consuming in the method that spectrophotometer respectively detects solution to be measured and reference solution respectively.
Summary of the invention
For this reason, it may be necessary to provide a kind of specimen holder for sample detection, to solve, measurement sample to be tested concentration is difficult to be asked
Topic.
To achieve the above object, a kind of specimen holder for sample detection is inventor provided, it is characterised in that: including purple
Outer light source, sample room, the first photomultiplier tube and the second photomultiplier tube, the ultraviolet source, the first photomultiplier tube with
And second photomultiplier tube be all set in outside sample room, in the sample room include beam splitter, reflecting mirror, the first sample cell and
Second sample cell, first sample cell is for containing reference liquid, and second sample cell is for containing testing sample solution, institute
Beam splitter is stated to be set on the line of ultraviolet source and the first sample cell, the beam splitter is obliquely installed, the reflecting mirror with point
The setting direction of Shu Jing is identical, and the reflecting mirror is set on the radiation direction that ultraviolet light reflects on beam splitter, and described second
Sample cell is set on the radiation direction reflected on reflecting mirror, the position of first photomultiplier tube and the position of the first sample cell
Set corresponding, the position of second photomultiplier tube is corresponding with the position of the second sample cell.
Further, the number of second sample cell is two or more, and second sample cell is all set in the first sample
Product pond it is ipsilateral, and the first sample cell arranges along the vertical direction with the second sample cell.
It further, further include the first sliding rail and the second sliding rail, the extending direction of first sliding rail and the second sliding rail is equal
Identical as the orientation of the second sample cell, the reflecting mirror is provided with the first sliding block compatible with the first sliding rail, and described
Two photomultiplier tubes are provided with the second sliding block compatible with the second sliding rail, and the reflecting mirror is by the first sliding block along the first sliding rail
Sliding, second photomultiplier tube are slided by the second sliding block along the second sliding rail.
Further, first sliding rail is identical as the length of the second sliding rail.
Further, it is provided with the first limiting section on first sliding rail, is provided with the second limit on second sliding rail
Portion, first limiting section are corresponding with the position of the second sample cell respectively with the position of the second limiting section.
Further, first limiting section and the second limiting section are limit holes, and first sliding block is sliding towards first
Side and the second sliding block in rail are provided with boss towards the side in the second sliding rail, and the boss is mutually fitted with limit hole
Match.
It further, further include visible light source and monochromator, the light that the ultraviolet source is issued by visible light source
Line is formed after decomposing through monochromator.
It further, further include amplifier, single-chip microcontroller and display, first photomultiplier tube and the second photoelectricity are again
Increase pipe and be all connected to amplifier, the amplifier is connected to display by single-chip microcontroller, has in the single-chip microcontroller for by first
Photomultiplier tube and the obtained electric signal of the second photomultiplier tube carry out the program that subtractive obtains testing sample solution absorbance.
Further, the beam splitter and the reflecting mirror are with horizontal line in 45 ° of settings.
Further, the beam splitter is set to the surface of reflecting mirror, and the first sample cell is set to same with beam splitter
On horizontal line, the second sample cell and the first sample cell are set on same vertical line.
It is different from the prior art, above-mentioned technical proposal has the advantages that using while being set to sample indoor first
Sample cell and the second sample cell detect the transmitted intensity of institute's subtractive between reference liquid and testing sample solution simultaneously, this is used for
The specimen holder structure of sample detection is simple, can carry out the detection of reference liquid and testing sample solution simultaneously, avoids constantly substituting sample
The indoor sample of product, reduces the influence of human factor, improves the stability of device, reduces experimental period, improves experiment
Efficiency, ensure that the accuracy and reliability of experimental result.
Detailed description of the invention
Fig. 1 is a kind of overall structure diagram of the specimen holder for sample detection of the present embodiment;
Fig. 2 is a kind of sample pool structure schematic diagram of the specimen holder for sample detection of the present embodiment.
Description of symbols:
1, sample room;
11, beam splitter;
12, reflecting mirror;
13, the first sample cell;
14, the second sample cell;
21, the first photomultiplier tube;
22, the second photomultiplier tube;
31, visible light source;
32, monochromator;
41, the first sliding rail;
411, the first limiting section;
42, the second sliding rail;
421, the second limiting section.
Specific embodiment
Technology contents, construction feature, the objects and the effects for detailed description technical solution, below in conjunction with specific reality
It applies example and attached drawing is cooperated to be explained in detail.
Fig. 1 and Fig. 2 are please referred to, the present invention provides a kind of specimen holder for sample detection, including ultraviolet source, sample room
1, the first photomultiplier tube 21 and the second photomultiplier tube 22, ultraviolet source provide ultraviolet light source, sample for the sample detection
For placing reference solution and testing sample solution, the first photomultiplier tube and the second photomultiplier tube will transmit through ginseng for product room
Optical signal than solution or testing sample solution is converted into electric signal, then accesses and calculate testing sample solution in computing module
Absorbance.The ultraviolet source, the first photomultiplier tube and the second photomultiplier tube are all set in outside sample room.
In certain preferred embodiments, which further includes visible light source 31 and monochrome
Device 32, the light that the ultraviolet source is issued by visible light source are formed after decomposing through monochromator.Monochromator is set to visible
Between radiant and sample room.
It include beam splitter 11, reflecting mirror 12, the first sample cell 13 and the second sample cell 14, beam splitter in the sample room
Also known as semi-transparent semi-reflecting reflecting mirror, the light being incident on beam splitter are divided into the equal two-beam of intensity, i.e. reflected light and transmitted light, instead
Penetrating the ratio between rate and transmissivity is 1:1, and in the present embodiment, the structure of beam splitter is the prism beam splitting for high performance optical systems
Mirror.Reflecting mirror is a kind of optical element to be worked using reflection law, is incident on the light on reflecting mirror after reflecting mirror with certain
Angle reflection, to change original optical path direction.First sample cell is used for containing reference liquid, second sample cell
In containing testing sample solution, using the first photomultiplier tube to the transmitted intensity of the reference liquid contained in the first sample cell into
Row detection, it is therefore intended that with the second photomultiplier tube to the transmitted intensity of the testing sample solution contained in the second sample cell into
Row calibration, so that it is determined that the absorbance of testing sample solution.The pond body side wall material of first sample cell and the second sample cell is can
Saturating ultraviolet light material, specifically can be and is made of quartz.
In the present embodiment, the height of light transmitted through the first sample cell or the second sample cell be below the first sample cell with
The liquid level of second sample cell, to guarantee that light transmits the first sample cell or when the second sample cell is perforated through and wherein contains
Reference liquid or testing sample solution.
In the present embodiment, with horizontal line in 45 ° of settings, beam splitter is set to instead for the beam splitter and the reflecting mirror
The surface of mirror is penetrated, the first sample cell and beam splitter are set in same horizontal line, and the second sample cell and the first sample cell are arranged
In on same vertical line, in this case, the reflected light of light and reflecting mirror through beam splitter can be along dampening
Horizontal line is transmitted into the first sample cell and the second sample cell.
In the present embodiment, first sample cell is integrated with the second sample cell, the first sample cell and second
Sample cell is in the same sample cell physically arranged, and integrated first sample cell and the second sample cell are consolidated by fixing piece
It is scheduled in sample room.In other embodiments, the first sample cell and the second sample cell are also possible to individual individual, the first sample
Pond and the second sample cell are arranged and are fixed in sample room by fixing piece.
The beam splitter is set on the line of ultraviolet source and the first sample cell, and the beam splitter is obliquely installed, described
Reflecting mirror is identical as the setting direction of beam splitter, and the reflecting mirror is set to the radiation direction that ultraviolet light reflects on beam splitter
On, second sample cell is set on the radiation direction reflected on reflecting mirror, the position of first photomultiplier tube and the
The position in a sample pond is corresponding, and the position of second photomultiplier tube is corresponding with the position of the second sample cell.
The ultraviolet light that ultraviolet source issues is incident on beam splitter, and it is equal that incident ultraviolet light is divided into intensity by beam splitter
Reflected light and transmitted light, transmitted light keep the optical path direction of former ultraviolet source to be incident on the first sample cell, reflection light direction and original
The optical path direction of ultraviolet source is perpendicular to be incident on reflecting mirror, and light is incident on the second sample after the reflection of reflecting mirror
Pond, reflecting mirror on reflected light back to the second sample cell, will be incident on the first sample cell and for changing the direction of reflected light
The light intensity of two sample cells is suitable, and optical path direction is parallel to each other, and the light by the first sample cell reference liquid does not change light
Road direction reaches the first photomultiplier tube, does not change optical path direction by the light of the second sample cell testing sample solution and reaches the
Two photomultiplier tubes.
When light is transmitted through the first sample cell and the second sample cell, light is absorbed and anti-by the pool wall institute of sample cell
The part penetrated is very little, and most light is through the reference liquid of the first sample cell or the testing sample solution of the second sample cell
After reach the first photomultiplier tube and the second photomultiplier tube, then by a photomultiplier tube and the second photomultiplier tube point
The transmitted intensity of liquid in the first sample cell and the second sample cell is not detected.
Further include amplifier, single-chip microcontroller and display in certain preferred embodiments, first photomultiplier tube with
And second photomultiplier tube be all connected to amplifier, the amplifier is connected to display by single-chip microcontroller, in the single-chip microcontroller
Have and show that testing sample solution is inhaled for the first photomultiplier tube and the obtained electric signal of the second photomultiplier tube to be carried out subtractive
The program of luminosity.After collected optical signal is converted into electric signal by the first photomultiplier tube and the second photomultiplier tube,
Amplifier amplifies the electric signal exported in the first photomultiplier tube and the second photomultiplier tube, and single-chip microcontroller is by first
Electric signal difference between photomultiplier tube and the second photomultiplier tube is aobvious by the absorbance that testing sample solution is calculated
It is shown on display.
In certain preferred embodiments, the number of second sample cell is two or more, and second sample cell is equal
It is set to the ipsilateral of the first sample cell, and the first sample cell arranges along the vertical direction with the second sample cell.For containing to test sample
Second sample cell number of product solution is set as more than two, can carry out a variety of samples on the specimen holder for sample detection
The detection of product realizes that various samples are put into primary load, and when effectively preventing detecting two or more samples, needs constantly more
Product are varied, detection efficiency is improved, in addition can also be prevented during constantly opening the specimen holder to the first sample cell
The volume of middle reference liquid influences.It is chased after in the stroke that the first sample cell and the second sample cell of vertical setting of types setting also facilitate reflecting mirror
Track.
It is highly preferred that the specimen holder for being used for sample detection includes the first sliding rail 41 and the second sliding rail 42, described first is sliding
The extending direction of rail and the second sliding rail is identical as the orientation of the second sample cell, i.e. the first sliding rail and the second sliding rail are also along perpendicular
For histogram to setting, the reflecting mirror is provided with the first sliding block compatible with the first sliding rail, the second photomultiplier tube setting
There is the second sliding block compatible with the second sliding rail, the reflecting mirror is slided by the first sliding block along the first sliding rail, second light
Electric multiplier tube is slided by the second sliding block along the second sliding rail.The length of first sliding rail and the second sliding rail and two the second samples end to end
Pond it is vertical apart from corresponding, it is highly preferred that in order to enable the light transmission of reflecting mirror reflection the second sample cell central part
Position, then the upper end of the first sliding rail and the second sliding rail is set to the middle position of second sample cell of the top, the first sliding rail and the
The lower end of two sliding rails is set to the middle position of the second sample cell of the lowermost, is conducive to save the first sliding rail and the second sliding rail
Use material.
In more preferred embodiment, first sliding rail is identical as the length of the second sliding rail.The beneficial effect is that
The specimen holder symmetrical configuration for sample detection is enabled to, it is more aesthetically pleasing.
In certain preferred embodiments, it is provided with the first limiting section 411 on first sliding rail, on second sliding rail
Be provided with the second limiting section 412, the position of first limiting section and the second limiting section respectively with the position phase of the second sample cell
It is corresponding.First limiting section and the second limiting section are respectively used to limit the position of reflecting mirror and the second photomultiplier tube, avoid
The position of reflecting mirror or the second photomultiplier tube changes during detection, to influence testing result.Work as reflecting mirror
When being moved up and down by the first sliding block along the first sliding rail, the first sliding block can be braked at the position of the first limiting section, when
When continuing to move to, which disappears.When the second photomultiplier tube is moved up and down by the second sliding block along the second sliding rail, the
Two sliding blocks are at the position of the second limiting section, it may occur that braking, when to be continued to move to, which disappears.
The structure of concrete implementation braking is: first limiting section and the second limiting section are limit holes, described first
Sliding block is provided with boss, the boss towards the side in the first sliding rail and the side in the second sliding block the second sliding rail of direction
It is adapted with limit hole.The size of boss is small in the size of the first sliding block and the second sliding block, and the first sliding block is in the first sliding rail
When upper sliding, when by the first limiting section, boss is caught in the first limiting section, and when to be continued to move to, reflecting mirror is lifted, the
The boss of one sliding block leaves the first limiting section, repeats this and operates many places positioning that reflecting mirror can be achieved;Second sliding block is sliding second
When sliding on rail, when by the second limiting section, boss is caught in the second limiting section, when to be continued to move to, by the second photoelectricity times
Increase Guan Tiqi, the boss of the second sliding block leaves the second limiting section, and it is fixed to repeat many places that this operates achievable second photomultiplier tube
Position.
It should be noted that being not intended to limit although the various embodiments described above have been described herein
Scope of patent protection of the invention.Therefore, it based on innovative idea of the invention, change that embodiment described herein is carried out and is repaired
Change, or using equivalent structure or equivalent flow shift made by description of the invention and accompanying drawing content, it directly or indirectly will be with
Upper technical solution is used in other related technical areas, is included within the scope of protection of the patent of the present invention.
Claims (10)
1. a kind of specimen holder for sample detection, it is characterised in that: including ultraviolet source, sample room, the first photomultiplier tube
And second photomultiplier tube, the ultraviolet source, the first photomultiplier tube and the second photomultiplier tube are all set in sample
Outdoor, includes beam splitter, reflecting mirror, the first sample cell and the second sample cell in the sample room, and first sample cell is used
In containing reference liquid, for second sample cell for containing testing sample solution, the beam splitter is set to ultraviolet source and the
On the line in a sample pond, the beam splitter is obliquely installed, and the reflecting mirror is identical as the setting direction of beam splitter, the reflection
Mirror is set on the radiation direction that ultraviolet light reflects on beam splitter, and second sample cell is set to the light reflected on reflecting mirror
On line direction, the position of first photomultiplier tube is corresponding with the position of the first sample cell, second photomultiplier tube
Position it is corresponding with the position of the second sample cell.
2. the specimen holder according to claim 1 for sample detection, it is characterised in that: the number of second sample cell
For two or more, second sample cell is all set in the ipsilateral of the first sample cell, and the first sample cell and the second sample cell edge
Vertical direction arrangement.
3. the specimen holder according to claim 2 for sample detection, it is characterised in that: further include the first sliding rail and second
The extending direction of sliding rail, first sliding rail and the second sliding rail is identical as the orientation of the second sample cell, the reflecting mirror
It is provided with the first sliding block compatible with the first sliding rail, second photomultiplier tube is provided with compatible with the second sliding rail
Two sliding blocks, the reflecting mirror are slided by the first sliding block along the first sliding rail, and second photomultiplier tube passes through the second sliding block edge
The sliding of second sliding rail.
4. the specimen holder according to claim 3 for sample detection, it is characterised in that: first sliding rail and second is slided
The length of rail is identical.
5. the specimen holder according to claim 3 for sample detection, it is characterised in that: be provided on first sliding rail
First limiting section is provided with the second limiting section, the position point of first limiting section and the second limiting section on second sliding rail
It is not corresponding with the position of the second sample cell.
6. the specimen holder according to claim 5 for sample detection, it is characterised in that: first limiting section and second
Limiting section is limit hole, in the side and the second sliding block the second sliding rail of direction in the first sliding rail of the first sliding block direction
Side is provided with boss, and the boss is adapted with limit hole.
7. the specimen holder according to claim 1 for sample detection, it is characterised in that: further include visible light source and
Monochromator, the light that the ultraviolet source is issued by visible light source are formed after decomposing through monochromator.
8. the specimen holder according to claim 1 for sample detection, it is characterised in that: further include amplifier, single-chip microcontroller
With display, first photomultiplier tube and the second photomultiplier tube are all connected to amplifier, and the amplifier passes through list
Piece machine is connected to display, has in the single-chip microcontroller for by the first photomultiplier tube and the obtained telecommunications of the second photomultiplier tube
Number carrying out subtractive obtains the program of testing sample solution absorbance.
9. the specimen holder according to claim 1 for sample detection, it is characterised in that: the beam splitter and the reflection
Mirror is with horizontal line in 45 ° of settings.
10. the specimen holder according to claim 1 for sample detection, it is characterised in that: the beam splitter is set to instead
The surface of mirror is penetrated, the first sample cell and beam splitter are set in same horizontal line, and the second sample cell and the first sample cell are arranged
In on same vertical line.
Priority Applications (1)
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CN201910059288.2A CN109772490A (en) | 2019-01-22 | 2019-01-22 | A kind of specimen holder for sample detection |
Applications Claiming Priority (1)
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CN201910059288.2A CN109772490A (en) | 2019-01-22 | 2019-01-22 | A kind of specimen holder for sample detection |
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CN109772490A true CN109772490A (en) | 2019-05-21 |
Family
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Citations (6)
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US20010013931A1 (en) * | 2000-02-14 | 2001-08-16 | Shimadzu Corporation | Double beam spectrophotometer |
CN201434821Y (en) * | 2009-07-24 | 2010-03-31 | 厦门大学 | Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer |
CN102788764A (en) * | 2012-08-21 | 2012-11-21 | 南京埃森环境技术有限公司 | Ultraviolet analyzer and detection method for low concentration smoke |
CN205038156U (en) * | 2015-10-09 | 2016-02-17 | 东莞立创华科检测技术服务有限公司 | Ultraviolet visible light spectrophotometer |
CN107941717A (en) * | 2017-11-20 | 2018-04-20 | 徐海峰 | A kind of static state Multi-example pond spectrophotometer |
CN209530923U (en) * | 2019-01-22 | 2019-10-25 | 福建师范大学 | A kind of specimen holder for sample detection |
-
2019
- 2019-01-22 CN CN201910059288.2A patent/CN109772490A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20010013931A1 (en) * | 2000-02-14 | 2001-08-16 | Shimadzu Corporation | Double beam spectrophotometer |
CN201434821Y (en) * | 2009-07-24 | 2010-03-31 | 厦门大学 | Ultraviolet-visible absorption accessory of Turner 10-AU field fluorophotometer |
CN102788764A (en) * | 2012-08-21 | 2012-11-21 | 南京埃森环境技术有限公司 | Ultraviolet analyzer and detection method for low concentration smoke |
CN205038156U (en) * | 2015-10-09 | 2016-02-17 | 东莞立创华科检测技术服务有限公司 | Ultraviolet visible light spectrophotometer |
CN107941717A (en) * | 2017-11-20 | 2018-04-20 | 徐海峰 | A kind of static state Multi-example pond spectrophotometer |
CN209530923U (en) * | 2019-01-22 | 2019-10-25 | 福建师范大学 | A kind of specimen holder for sample detection |
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