CN201431512Y

CN201431512Y - Rat sleep deprivation automatic record test device

Info

Publication number: CN201431512Y
Application number: CN2009200591932U
Authority: CN
Inventors: 彭康; 朱传武; 许文学; 刘海弘
Original assignee: Southern Medical University
Current assignee: Southern Medical University
Priority date: 2009-06-25
Filing date: 2009-06-25
Publication date: 2010-03-31
Anticipated expiration: 2019-06-25

Abstract

The utility model discloses a rat sleep deprivation automatic record test device, which comprises an automatic record circuit and a water container (1), wherein the middle of the water container (1) is provided with a flower pot (2) which extends out of water level and is used for a rat to stand, and the utility model is characterized in that the flower pot (2) extends downward to the bottom of the water container (1) from the pot surface to be fixedly connected with the bottom of the water container (1), a reflective photoelectric switch (3) penetrates from the bottom of the water container (1) to the part of the flower pot (2), which extends out of the water level, and the light-sensitive surface is exposed, and the output end extends from the bottom of the water container. The utility model has the obvious effects of correct judgment and accurate record.

Description

A kind of rat sleep deprivation writes down experimental provision automatically

Technical field

This utility model relates to the instrument that is specifically designed to animal, is specifically related to rat sleep deprivation experimental provision.

Background technology

(Sleep deprivation SD) is usually used in studying the influence of some drugs to animal behavior to sleep deprivation, as the research to the rat behavior influence of rat irritability and exploratory melatonin.At present, often adopt chain-wales water environment method (flower pots technique) to set up the sleep deprivation model.The method mainly is the life habit of utilizing rat fear water and can't enter sleep in water, by placement platform in the tank that is filled with water, allow rat stand on the platform, because of platform diameter enough little (diameter 4.5cm or littler), if the Mus drinking-water of can taking food voluntarily on platform is its sleep, then because muscle tone relaxes falls into the water, rat can only come up smiling and climb up platform, so reaches the effect of sleep deprivation repeatedly.But, because the current experiments device is simpler and cruder, obviously have following deficiency: needs are omnidistance when 1, testing monitors, and require experimenter's tool to have a determined will and the sense of responsibility of height, otherwise just can't obtain the drowning number of times of rat, at every turn in holdup time on the chain-wales and the data such as soak time in water; 2, manual record data; It is accurately relatively low; 3, recorded data needs artificial input computer to carry out computing, and is not only loaded down with trivial details but also time-consuming.

State Intellectual Property Office disclosed the application for a patent for invention of a kind of " multi-functional instrument for testing fatigue of animals " on June 14th, 2006, and (publication number is: CN 1785135A), the technical scheme of this patent application is: in the experiment water drum montant is set, the upper end of this montant is bearing on the spring that is fixed on the barrel, the pairing barrel in the top of described montant is established a photoelectric sensor, and a thin cross bar for the white mice climbing is established at the middle part.In the time of in white mice is put into the experiment water drum that water is housed, just can climbs up on the described thin cross bar and seek survival.At this moment, under the effect of white mice body weight, described montant overcomes the elastic force of spring and moves down; Because cross bar is thinner, white mice can be owing to the fatigue of standing be fallen into water, and described montant just resets under the effect of spring.As seen, so constantly repeat, also can obtain the drowning number of times, each of Mus by photoelectric sensor in holdup time on the chain-wales and the data such as soak time in water, but, remain in following deficiency: 1, the reciprocating mechanism more complicated that constitutes by montant, thin cross bar and spring, especially control montant by the body weight of laboratory animal and pump, therefore will select the suitable spring of coefficient of elasticity for use, therefore obviously do not possess versatility according to the body weight of laboratory animal; 2, montant and thin cross bar are vertical, and the body weight of laboratory animal must produce perpendicular to montant component, and this makes the frictional force increase between described montant and the guider and makes reciprocating mechanism owe is obvious flexibly; 3, because being the number of times that moves up and down that detects montant, photoelectric sensor come the indirect detection Mus to climb up thin cross bar number of times, therefore as long as acting on thin cross bar, the body weight of Mus will cause montant to move down and count, the accuracy of its counting obviously reduces, and makes montant move down i.e. generation erroneous judgement on the cross bar because the extremity of Mus act on momently; 4, white mice causes the thin cross bar of described reciprocating mechanism to rock when climbing on thin cross bar easily, just is good at climbing up when white mice is tired, and the sleep deprivation effect obviously reduces.

Summary of the invention

In view of there is above-mentioned deficiency in prior art, the technical problems to be solved in the utility model is to improve the accuracy and the reliability of sleep deprivation experimental data.

The technical scheme that this utility model addresses the above problem is:

A kind of rat sleep deprivation writes down experimental provision automatically, this device comprises automatic writing circuit and water container, the middle part of described water container is provided with an emersed chain-wales that supplies rat to stand, it is characterized in that described chain-wales is also fixedly connected with it from the bottom that table top extends to water container vertically downward; One reflective photoelectric switch is worn to the emersed part of described chain-wales by the bottom of water container, and its photosurface appears, and outfan is stretched out by the bottom of water container.

Experimental provision described in the utility model, wherein, described water container can be the pond of different shape, when it adopts clear glass to make, with quadrate be good; The plastic round tube that described chain-wales preferably selects for use aperture and reflective photoelectric switch external diameter to be complementary is made, and the common glue (getting glue as hundred) of so easy employing is adhesively fixed.For the photosurface of protecting reflective photoelectric switch not contaminated; the head of described plastic round tube is preferably established a transparent protective cover; this over cap is connected with the head threads of plastic round tube; it is crown that end face is designed to raise up a little; this measure both can allow rat flow back in the water container with the sewage that gets on; also be convenient to frequent cleaning, be beneficial to the sensitivity and the reliability of its work of assurance.

Experimental provision described in the utility model, the oral area of described water container is provided with cover plate, and this cover plate is provided with air-vent, and be advisable with the finger that can insert the adult in this aperture, and it can bear the effect of the control member of opening cover plate like this.

Finish described self registering invention task, experimental provision described in the utility model obviously also should comprise the signal of telecommunication that picks up reflective photoelectric switch and write down the automatic writing circuit of interval of its number of times opening and close and Push And Release, but, be described so need not to take bigger length because this circuit is that those of ordinary skills are easy to realize.

Experimental provision described in the utility model is existing to have following beneficial technical effects: 1, whole sensing device is the structure of a solid envelope, not only simply but also can be not malfunctioning; 2, reflective photoelectric switch is directly accepted the optical signal by the reflection of rat health, and is accurate; 3, described chain-wales maintains static, and can not fall into water because of it rocks once again after rat climbs up, and has only when rat is slept just can fall into the water because of muscle tone is lax, obviously can not cause erroneous judgement; 4, the fixed advantage again of described chain-wales is, rat can climb to get on because of tired, and the sleep deprivation effect significantly improves.

Description of drawings

Fig. 1 is the structural representation of a specific embodiment of water container in the experimental provision described in the utility model.

Fig. 2 is the structural representation of local I among Fig. 1, demonstrates a kind of concrete structure of chain-wales described in the utility model among the figure.

Fig. 3 is the electrical schematic diagram of a specific embodiment of automatic writing circuit in the experimental provision described in the utility model.

The specific embodiment

As shown in Figure 1, the cuboid tank of water container 1 for adopting bonding glass to cause; Chain-wales 2 usefulness pvc pipes are made, and are located at the central authorities of described tank, are passed by the bottom of tank; Reflective photoelectric switch 3 is worn to top by the bottom of pvc pipe, its photosurface is concordant with the upper surface of pvc pipe, admittedly seal with epoxy resin all around, the top of described pvc pipe is with the transparent protective cover 4 that is threaded, with the sensitization cover of reflective photoelectric switch 3 (referring to Fig. 2) within it, it is crown that the end face of described transparent protective cover 4 is designed to raise up a little; The oral area that constitutes the cuboid tank of water container 1 is established the cover plate 5 of glass, and this cover plate 5 is provided with air-vent 6, and the aperture of this air-vent 6 is slightly larger than into the diameter of finger.

Referring to Fig. 1, the bottom that constitutes the cuboid tank of water container 1 is provided with a support 8, and these support 8 usefulness aluminium section bars cause the shape of common stool, and whole water container 1 is placed on the stool face of hollow, and described pvc pipe extends the bottom of stool face.

Referring to Fig. 3, writing circuit is by commercially available Programmable Logic Controller K7M-DR20UE, text display MD204L, 5 reflective photoelectric switch SENSOR1～SENSOR5 and 24V dc source 24VPOWER connect to form, wherein, 5 reflective photoelectric switch SENSOR1～SENSOR5 are connected with the switching signal input port of Programmable Logic Controller K7M-DR20UE respectively, the RS232 PORT mouth of described text display MD204L docks with the RS232 PORT of Programmable Logic Controller K7M-DR20UE, and described dc source 24VPOWER is text display MD204L and 5 reflective photoelectric switch SENSOR1～SENSOR5 power supply and by com port (specifically selecting COM port or COM device or COM1 among Fig. 3 for use) and Programmable Logic Controller K7M-DR20UE altogether.

Writing circuit shown in Figure 3 is No. 5 writing circuits, can be connected with set reflective photoelectric switch 3 in 5 water containers shown in Figure 11, promptly constitutes the sleep deprivation experiment that can carry out 5 rats simultaneously.The Method and circuits work process of experiment is briefly described below:

Output interface 7 with reflective photoelectric switch 3 (one of SENSOR1～SENSOR5 among Fig. 3) is connected with arbitrary switching signal input port of Programmable Logic Controller K7M-DR20UE earlier; in water container 1, inject the cleandrinking water of about 20 degree of warm water degree again; make water level to the about 2cm of the upper surface of transparent protective cover 4, connect the alternating current power supply (see figure 3) of Programmable Logic Controller K7M-DR20UE and dc source 24VPOWER then.After preparation is carried out, experimental rat put into water container 1 and cover glass cover-plate 5 and can begin experiment.Because rat fear water can be climbed up the upper surface of the transparent protective cover 4 that is threaded with chain-wales 2 rapidly, this moment, reflective photoelectric switch 3 transferred closure state to by disengaged condition, promptly sent a high level pulse to Programmable Logic Controller K7M-DR20UE; Because transparent protective cover 4 faces on the chain-wales 2 are very little; rat can only be stood on it; in case during sleep; then fall into the water owing to muscle tone is lax; this moment, 3 of reflective photoelectric switches were disengaged condition by the closure state redirect, promptly sent a low level pulse to Programmable Logic Controller K7M-DR20UE.Climb up in the repetitive process of transparent protective cover 4 on the chain-wales 2 of transparent protective cover 4 on the chain-wales 2 → fall into the water → climb up again → fall into the water again rat; 3 of reflective photoelectric switches are closure state by the normally open redirect repeatedly correspondingly; be disengaged condition by the closure state redirect again; Programmable Logic Controller K7M-DR20UE just writes down the closure of reflective photoelectric switch 3 respectively and throws off number of times and each time by to throw off redirect be the interval of closure state and be the interval of disengaged condition by the closure state redirect, so ceaselessly periodic duty can be finished the experiment of rat sleep deprivation.

Claims

1, a kind of rat sleep deprivation writes down experimental provision automatically, this device comprises automatic writing circuit and water container (1), the middle part of described water container (1) is provided with an emersed chain-wales (2) that supplies rat to stand, it is characterized in that described chain-wales (2) is also fixedly connected with it from the bottom that table top extends to water container (1) vertically downward; One reflective photoelectric switch (3) is worn to the emersed part of described chain-wales (2) by the bottom of water container (1), and its photosurface appears, and outfan is stretched out by the bottom of water container.

2, experimental provision according to claim 1 is characterized in that, the plastic round tube that described chain-wales (2) aperture and reflective photoelectric switch external diameter are complementary, the head of this plastic round tube are established a transparent protective cover (4).

3, experimental provision according to claim 2 is characterized in that the end face of described transparent protective cover (4) is raise up a little crown.

According to claim 1,2 or 3 described experimental provisions, it is characterized in that 4, the oral area of described water container (1) is provided with cover plate (5), this cover plate (5) is provided with air-vent (5), and the aperture of described air-vent (5) is slightly larger than the diameter of adult's finger.