CN201255668Y - Enzyme type time-temperature indication card - Google Patents
Enzyme type time-temperature indication card Download PDFInfo
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- CN201255668Y CN201255668Y CNU2008201858734U CN200820185873U CN201255668Y CN 201255668 Y CN201255668 Y CN 201255668Y CN U2008201858734 U CNU2008201858734 U CN U2008201858734U CN 200820185873 U CN200820185873 U CN 200820185873U CN 201255668 Y CN201255668 Y CN 201255668Y
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Abstract
The utility model discloses an enzyme time-temperature indicator card which comprises an outer package, i.e. a transparent container; the container is provided with two sealed small pipes, to divide the container into three cavities which are not communicated; wherein, the cavity (1) contains alkaline lipase; the cavity (2) contains glyceride; the cavity (3) contains buffer solution and pH indicator. The two sealed small pipes are provided with easily broken parts on a plane; the solution in the cavity (1) and the cavity (2) is mixed into the cavity (3) by breaking the two small pipes. The enzyme time-temperature indicator card uses the hydrolysis of the lipase and the glyceride to drop the pH of a reaction system, and displays the cumulative effect of the time and the temperature through color changes with the pH indicator as the indicator. In addition, the time-temperature indicator card also works together with a colorimetric card. The enzyme time-temperature indicator card can accurately indicate the cumulative effect of the time and the temperature of objects in production, storage, transportation and sales.
Description
One, technical field
The utility model relates to a kind of enzyme type time-temperature indication card, can be used for indicating the cumulative effect of article time-temperature in production, storage, transportation and sales process, and can indicate the quality of product to a certain extent.
Two, background technology
The food quality safety problem occurs again and again, has caused people's extensive concern.Food quality is except that waited internal factor influences by himself composition, also be subjected to the influence of external environment conditions such as atmospheric pressure, mechanical pressure, illumination, temperature, and quality security problem often occurs in production storage, transportation or the shelf life of food, is a very important link so control external environment condition well.In all external environment influence factors, temperature is a factor that unpredictability is higher, often fluctuates because of the variation of environment, and the temperature control in the storage environment is improper, will cause food apoilage, and cause economic loss.Have because the quality of product is also relevant with the time of storing, under certain temperature condition, period of storage is long more, and its quality is poor more.When two factors of temperature and time that circulate are accumulated in together, just determined the quality of this product.Therefore need a kind of effective means to come the time of monitoring product in the process of circulation and the accumulation of temperature, indicate the quality information of product simultaneously, the information of the remaining shelf life of product is provided.
Three, summary of the invention
The purpose of this utility model is: at the deficiencies in the prior art part, the utility model provides the enzyme type time-temperature indicating card of the cumulative effect of a kind of people's of informing product time-temperature in production, storage, transportation and sales process.
The purpose of this utility model is achieved in that a kind of enzyme type time-temperature indication card, it is characterized in that: external packing is a transparent container, also be provided with tubule (4) and (5) of 2 sealings in this container, thereby container is separated into three three cavitys that do not communicate, be respectively cavity (1), cavity (2) and cavity (3), wherein in cavity (1) alkaline lipase is housed, cavity is equipped with glyceride in (2), and buffer solution and pH indicator are housed in the cavity (3).Described indicating card also comprises supporting Standard Colors colorimetric card.Two sealing tubules (4) and (5) of indicating card in one plane have an easily broken place (6), and two easily broken places of tubule (6) are mixed into the solution in cavity (1) and the cavity (2) in the cavity (3) by fractureing.Described indicating card also is included in outer containter and sets up a color colorimetric card outward separately.
During actual the use, with this time-the temperature sticker is in the external packing of thermally sensitive article, time-temperature indication card and identical whole temperature variation that article will experience contrast time of being experienced and temperature variation that the color colorimetric card just can be known article so.
Advantage of the present utility model is: alkaline lipase is catalyzing glycerol ester generation hydrolysis reaction under alkaline environment, produces fatty acid, thereby changes the pH of reaction environment, just can reflect the intensity of variation of the pH of reaction system again by the pH indicator.Enzymic catalytic reaction is subjected to Temperature Influence, and within the specific limits, temperature is high more, and the speed of reaction is fast more, and the variation of pH is also just fast more, so the reactant liquor change in color will be accelerated.Because the degree of reaction also is subjected to the influence of time, under the uniform temperature condition, the time that reaction is carried out is long more again, and product is just many more, and the degree that pH descends is also just big more, and therefore, the reactant liquor change in color is also just big more.Therefore change in color is the function of time and temperature.The color and the colorimetric card of reactant liquor are compared the cumulative effect that just can judge the time-temperature of this product.
Four, description of drawings
Fig. 1 is the complete section structural representation of time-temperature indication card of the present utility model
1-cavity among the figure, 2-cavity, 3-cavity, 4-tubule, 5-tubule, 6-easily broken place.
Five, embodiment
Embodiment 1:
With reference to Fig. 1, embodiment of the present utility model is elaborated.Fig. 1 has provided a kind of enzyme type time-temperature indication card complete section structural representation, it comprises that external packing is a transparent container, also be provided with the tubule 4 and the tubule 5 of 2 sealings in this container, thereby container is separated into three three cavitys that do not communicate, be respectively cavity 1, cavity 2 and cavity 3, it is 1gL that concentration wherein is housed in cavity 1
-1Alkaline fat enzyme solutions 0.1mL, reaction substrate glyceryl triacetate 0.5mL is housed in the cavity 2, concentration is 20gL
-1Emulsifying agent PVA9.5mL; Concentration is 1molL
-1CaCl
2Solution 0.7mL; It is 0.05molL that concentration is housed in the cavity 3
-1, pH 10.6 Gly-NaOH buffer solution 39mL; 0.2mL phenol red-phenolphthalein-the thymolphthalein mixed solution (take by weighing that 0.1g is phenol red, 0.5g phenolphthalein and 0.2g thymolphthalein, be dissolved in 60% the ethanol of 100mL, filter, normal temperature is preserved down) is as the indicator buffer solution and the pH indicator of reaction.
Two of indicating card sealing tubules 4 and tubule 5 in one plane have an easily broken place 6, and two tubules are mixed into the solution in cavity 1 and the cavity 2 in the cavity 3 by fractureing.
This indicating card can also comprise supporting Standard Colors colorimetric card or set up a supporting Standard Colors colorimetric card separately outside outer containter.
Implementation result
Reaction system is reacted under different temperatures, and its change color trend is consistent, presents darkviolet-purple-wind rose-pink-rose pink-claret-orange-yellow-yellow variation tendency.And under 4,10 and 15 ℃ temperature of reaction, the color of reaction system rests on the stage of pale pink, has only when temperature arrives 20 ℃, has only when temperature arrives more than 20 ℃, claret, orange-yellow, yellow, wherein claret just can appear in reaction system: brightness value L
*Be 48.2～51.3; Redness degree a
*Value is 31.3～35.6; Yellow value degree b
*Be 15.4～17.2; Corresponding reaction time and temperature of reaction are 20 ℃, 26～34 hours; 25 ℃, 15～20 hours; 30 ℃, 10～13 hours; Reaction system occurs orange-yellow: brightness value L
*Be 56.0～57.8; Redness degree a
*Value is 11.5～12.9; Yellow value degree b
*Be 28.3～29.7; Corresponding reaction time and temperature of reaction are 20 ℃, 48～56 hours; 25 ℃, 24～28 hours; 30 ℃, 20～22 hours; Reaction system occurs yellow: brightness value L
*Be 60.9～63.1; Redness degree a
*Value is for-2.0～-0.9; Yellow value degree b
*Be 40.8～43.2; Corresponding reaction time and temperature of reaction are 20 ℃, 96～105 hours; 25 ℃, 52～60 hours; 30 ℃, 44～50 hours.
Above-mentioned result of implementation color value can also be made the supporting use of Standard Colors colorimetric card, perhaps outside outer containter, set up a supporting Standard Colors colorimetric card to use separately.
During actual the use, with this time-the temperature sticker is in the external packing of thermally sensitive article, time-temperature indication card and identical whole temperature variation that article will experience contrast time of being experienced and temperature variation that the color colorimetric card just can be known article so.
Time-temperature indication card of the present utility model, before the use, alkaline lipase, glyceryl triacetate and pH indicator and buffer solution are to be contained in respectively in three cavitys that do not communicate, and prevent that it from contacting with each other and react, thereby guarantee this indicating card quality before use.When using, can use external force that two tubules are fractureed, thereby glyceryl triacetate, alkaline lipase, buffer solution and pH indicator are mixed, begin to react.People can according to the color of solution judge product the accumulation of temperature-time of process.Time-temperature indication card of the present invention, easy to use, cost is low and accuracy is high can be used for indicating the cumulative effect of product at production, storage, transportation and sales process time-temperature, and then guarantee the quality and safety of product.
At last, it is also to be noted that what more than enumerate only is a specific embodiment of the present utility model.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection domain of the present utility model.
Claims (4)
1, a kind of enzyme type time-temperature indication card, it is characterized in that: external packing is a transparent container, be provided with tubule (4) and (5) of 2 sealings in this container, tubule (4) and (5) are separated into three cavitys that do not communicate with container, be respectively cavity (1), cavity (2) and cavity (3), wherein in cavity (1) alkaline lipase is housed, cavity is equipped with glyceride in (2), and buffer solution and pH indicator are housed in the cavity (3).
2, a kind of enzyme type time-temperature indication card according to claim 1 is characterized in that: described indicating card also comprises supporting Standard Colors colorimetric card.
3, enzyme type time-temperature indication card according to claim 1 and 2, it is characterized in that: two sealing tubules (4) and (5) in one plane have an easily broken place (6), and two easily broken places of tubule (6) are mixed into the solution in cavity (1) and the cavity (2) in the cavity (3) by fractureing.
4, enzyme type time-temperature indication card according to claim 1 is characterized in that: set up a Standard Colors colorimetric card outside outer containter separately.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201858734U CN201255668Y (en) | 2008-09-12 | 2008-09-12 | Enzyme type time-temperature indication card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201858734U CN201255668Y (en) | 2008-09-12 | 2008-09-12 | Enzyme type time-temperature indication card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201255668Y true CN201255668Y (en) | 2009-06-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201858734U Expired - Lifetime CN201255668Y (en) | 2008-09-12 | 2008-09-12 | Enzyme type time-temperature indication card |
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| CN (1) | CN201255668Y (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495052A (en) * | 2011-12-02 | 2012-06-13 | 江南大学 | Saccharifying enzyme type time-temperature indicator |
| CN103728297A (en) * | 2014-01-17 | 2014-04-16 | 石河子大学 | Application of time-temperature indicator system and device in production, storage and transportation of cold fresh mutton |
| CN106404626A (en) * | 2016-10-28 | 2017-02-15 | 清华大学 | PM2.5 mask service life indicator card |
| CN106501142A (en) * | 2016-10-28 | 2017-03-15 | 清华大学 | A kind of PM2.5 masks that may indicate that the life-span |
| WO2017161869A1 (en) * | 2016-03-23 | 2017-09-28 | 江南大学 | Solid enzyme-type time-temperature indicator |
| CN107978220A (en) * | 2017-11-07 | 2018-05-01 | 阜阳师范学院 | A kind of intelligence egg shelf-life indicating label |
| WO2020207023A1 (en) * | 2019-11-08 | 2020-10-15 | 中国科学院华南植物园 | Fruits and vegetables shelf life and quality prediction method |
-
2008
- 2008-09-12 CN CNU2008201858734U patent/CN201255668Y/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495052A (en) * | 2011-12-02 | 2012-06-13 | 江南大学 | Saccharifying enzyme type time-temperature indicator |
| CN103728297A (en) * | 2014-01-17 | 2014-04-16 | 石河子大学 | Application of time-temperature indicator system and device in production, storage and transportation of cold fresh mutton |
| CN103728297B (en) * | 2014-01-17 | 2016-01-06 | 石河子大学 | Time-temperature directive system and the application of device in cold fresh mutton producing and casting |
| WO2017161869A1 (en) * | 2016-03-23 | 2017-09-28 | 江南大学 | Solid enzyme-type time-temperature indicator |
| CN106404626A (en) * | 2016-10-28 | 2017-02-15 | 清华大学 | PM2.5 mask service life indicator card |
| CN106501142A (en) * | 2016-10-28 | 2017-03-15 | 清华大学 | A kind of PM2.5 masks that may indicate that the life-span |
| CN107978220A (en) * | 2017-11-07 | 2018-05-01 | 阜阳师范学院 | A kind of intelligence egg shelf-life indicating label |
| WO2020207023A1 (en) * | 2019-11-08 | 2020-10-15 | 中国科学院华南植物园 | Fruits and vegetables shelf life and quality prediction method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: JINHU FURUN POULTRY INDUSTRY FOOD CO., LTD. Assignor: Nanjing Agricultural University Contract record no.: 2014320000185 Denomination of utility model: Enzyme type time-temperature indication card production method Granted publication date: 20090610 License type: Common License Record date: 20140307 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090610 |