CN201245642Y - 膜状固定化酵母细胞乙醇连续发酵装置 - Google Patents

膜状固定化酵母细胞乙醇连续发酵装置 Download PDF

Info

Publication number
CN201245642Y
CN201245642Y CN 200820133729 CN200820133729U CN201245642Y CN 201245642 Y CN201245642 Y CN 201245642Y CN 200820133729 CN200820133729 CN 200820133729 CN 200820133729 U CN200820133729 U CN 200820133729U CN 201245642 Y CN201245642 Y CN 201245642Y
Authority
CN
China
Prior art keywords
yeast cell
utility
immobilized yeast
model
reactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200820133729
Other languages
English (en)
Inventor
周剑平
王治业
孙智敏
魏甲乾
杨晖
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN 200820133729 priority Critical patent/CN201245642Y/zh
Application granted granted Critical
Publication of CN201245642Y publication Critical patent/CN201245642Y/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/20—Heating; Cooling

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本实用新型公开了一种膜状固定化酵母细胞乙醇连续发酵装置，以解决现有生物反应器结构复杂、制备成本较高的问题。本实用新型包括预热池、进料管、酒醪收集罐、回流管、温度计，预热池通过进料管与柱式反应器相连，膜状固定化酵母细胞涂在不锈钢丝网上，卷成桶状置于柱式反应器中。本实用新型采用多级柱式生物反应器系统，将膜状固定化酵母细胞固定在不锈钢丝网上，卷成桶状置于反应器中，多级串联形成固定化细胞连续乙醇发酵装置，具有结构简单、生产效率高、发酵时间短、费用低及杂菌污染较小等优点。

Description

膜状固定化酵母细胞乙醇连续发酵装置
技术领域
本实用新型涉及一种生物反应器，尤其是一种膜状固定化酵母细胞乙醇连续发酵装置。
背景技术
生物反应器是利用生物催化剂进行化学反应的设备，是实现生物技术产品工业化制备的重要技术之一，有如下优点：可实现连续化、大型化和高度自动化生产；固定化细胞生物反应器由于集中了高浓度细胞，可提高生物反应速度，缩短生产周期；能实现产物与底物的及时分离；生产过程中可避免杂菌的污染等。
根据催化剂的分布情况可将固定化细胞生物反应器分为填充床、流化床、悬浮床、中空纤维式、膜式及管式等几种，但都存在有不同程度的缺陷，如填充床填充床固定化细胞反应器的混合度较差；流化床反应器难于扩大生产规模，且费用较高；悬浮床生物反应器不易于生产放大，费用较高。
现有多层分隔式生物反应器，是在发酵罐中置有由金属或硬质塑料及树脂等材料制的筛网粒子筐，粒子筐中放入固定化酵母凝胶体。现有反应器结构复杂，制备成本较高。
实用新型内容
本实用新型的目的是提供一种膜状固定化酵母细胞乙醇连续发酵装置，以解决现有生物反应器结构复杂、制备成本较高的问题。
本实用新型包括预热池、进料管、酒醪收集罐、回流管、温度计，所述预热池通过进料管与柱式反应器相连，膜状固定化酵母细胞涂在丝网上，卷成桶状置于柱式反应器中。
本实用新型采用多级柱式生物反应器系统，将膜状固定化酵母细胞固定在不锈钢丝网上，卷成桶状置于反应器中，多级串联形成固定化细胞连续乙醇发酵装置，具有结构简单、生产效率高、发酵时间短、费用低及杂菌污染较小等优点。
下面结合附图对本实用新型作进一步详细的说明。
附图说明
图1是本实用新型的结构示意图。
具体实施方式
本实用新型包括预热池1、进料管2、酒醪收集罐6、回流管7、温度计8，预热池1通过进料管2与柱式反应器相连，膜状固定化酵母细胞9涂在不锈钢丝网5上，卷成桶状置于柱式反应器中。多级串联进行酒精发酵。
柱式反应器为2～4个，相邻柱式反应器之间设有蠕动泵3。
使用时，将包埋有酵母细胞的聚乙烯醇复合凝胶铺在不锈钢丝网5上，冷冻成型后卷成桶状，置于柱式反应器的隔板上；然后将玉米糖化液泵入预热池1中，在本实施方式中柱式反应器为2个，预热后的糖液流入柱式反应器一(4-1)中，然后再将柱式反应器一(4-1)中的糖液通过蠕动泵3泵入到柱式反应器二(4-2)中，在柱式反应器通入无菌空气，然后进行酒精发酵。

Claims (2)

1、一种膜状固定化酵母细胞乙醇连续发酵装置，包括预热池、进料管、酒醪收集罐、回流管、温度计，其特征在于：所述预热池(1)通过进料管(2)与柱式反应器相连，膜状固定化酵母细胞(9)涂在丝网(5)上，卷成桶状置于柱式反应器中。
2、根据权利要求1所述的膜状固定化酵母细胞乙醇连续发酵装置，其特征在于：所述柱式反应器为2～4个，相邻柱式反应器之间设有蠕动泵(3)。
CN 200820133729 2008-08-29 2008-08-29 膜状固定化酵母细胞乙醇连续发酵装置 Expired - Fee Related CN201245642Y (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200820133729 CN201245642Y (zh) 2008-08-29 2008-08-29 膜状固定化酵母细胞乙醇连续发酵装置

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200820133729 CN201245642Y (zh) 2008-08-29 2008-08-29 膜状固定化酵母细胞乙醇连续发酵装置

Publications (1)

Publication Number Publication Date
CN201245642Y true CN201245642Y (zh) 2009-05-27

Family

ID=40729462

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200820133729 Expired - Fee Related CN201245642Y (zh) 2008-08-29 2008-08-29 膜状固定化酵母细胞乙醇连续发酵装置

Country Status (1)

Country Link
CN (1) CN201245642Y (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103756887A (zh) * 2013-12-31 2014-04-30 江苏元洲生物工程有限公司 连续流发酵罐

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103756887A (zh) * 2013-12-31 2014-04-30 江苏元洲生物工程有限公司 连续流发酵罐

Similar Documents

Publication Publication Date Title
Nagashima et al. Continuous ethanol fermentation using immobilized yeast cells
CN103305417A (zh) 进行蛋白生产的高产量反应器及其生产方法和应用
Gu et al. Effects of propionic acid on propionibacteria fermentation
CN101748054B (zh) 微藻培养的光生物反应器
CN203546025U (zh) 封闭式同步糖化乳酸生产线
CN103333787A (zh) 一种填料塔式连续表面发酵装置及其实现方法
CN101358169B (zh) 一种新型发酵罐
CN201272805Y (zh) 一种新型发酵罐
CN201245642Y (zh) 膜状固定化酵母细胞乙醇连续发酵装置
WO2008091113A1 (en) Multi-stage cstr bioreactor system equipped with cell recycle unit
CN102559783B (zh) 利用多联纤维床生物反应器系统发酵生产丁酸的方法
CN106906253A (zh) 一种利用表面固定化技术发酵生产l‑乳酸的方法
CN101492641A (zh) 膜状固定化酵母细胞乙醇连续发酵装置及其发酵方法
CN203373351U (zh) 一种程序化控制的驯化筛选培养设备
CN103952306B (zh) 一种使用超声波消泡的发酵罐
US9534236B2 (en) Membranes for wastewater-generated energy and gas
CN202164294U (zh) 一种固定化酵母细胞连续酒精发酵装置
CN110157603A (zh) 一种固定化黑曲霉产柠檬酸的发酵反应器及其方法
CN109868208A (zh) 菌藻一体式生物产能装置
Ali et al. Different type of industrial fermentors and their associated operations for the mass production of metabolite
CN201924016U (zh) 一种厌氧菌富集装置
CN109988757A (zh) 一种表面固定化酵母半连续发酵制备乙醇的方法
CN207828299U (zh) 一种连续生产乙醇的装置
CN105273994A (zh) 一种合成气厌氧发酵用塔式反应器
CN103865970A (zh) 固定化细胞连续生产单葡萄糖醛酸基甘草次酸的工艺及装置

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Assignee: Kunlun Biochemistry Co., Ltd., Gansu

Assignor: Zhou Jianping

Contract record no.: 2011640000006

Denomination of utility model: Continuous fermentation apparatus and method for membrane shaped immobilized yeast cell ethyl alcohol

Granted publication date: 20090527

License type: Exclusive License

Record date: 20110811

C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090527

Termination date: 20100829