CN201243574Y - Device for cultivation and preservation of cornea with perfusion - Google Patents
Device for cultivation and preservation of cornea with perfusion Download PDFInfo
- Publication number
- CN201243574Y CN201243574Y CNU2008200267065U CN200820026706U CN201243574Y CN 201243574 Y CN201243574 Y CN 201243574Y CN U2008200267065 U CNU2008200267065 U CN U2008200267065U CN 200820026706 U CN200820026706 U CN 200820026706U CN 201243574 Y CN201243574 Y CN 201243574Y
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- cornea
- bottle
- base
- bolt
- cultivation
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Abstract
A perfusion cultivation storage device of a cornea relates to the technical field of a medical apparatus, and comprises a bottle hood 7, a bottle body 1, a fixing rod 3, a base 4, a water inlet tube 5, a water outlet tube 6 and a bolt 8, wherein the fixing rod 3 and the base 4 are fixed on the bottle body 1; the bolt 8 can upwards and downwards slide along the fixing rod 3; and the bottle hood 7 covers the bottle body 1 through a notch 2. The utility model has the advantages that the device can carry out automatic liquid change, can provide sufficient nutrient content through continuously filling substrates to the device, brings metabolites in a circulation form, and maintains the relatively steady concentration and PH value of the cultivation liquid. Meanwhile, the operation procedure is simplified by a programming and closing system, the labour force consumption is largely reduced, and pollution from external environment is reduced. The cornea is placed between the bolt and the base to form a steady supporting point for fixing so that the cornea is convenient to transport and deliver. The device is made of transparent materials and is easy to observe the endothelial cells of the cornea in the cultivation process through converting a phase contrast microscope.
Description
Technical field:
The present invention relates to the medical device technical field, particularly relate to a kind of cornea perfusion and cultivate save set.
Background technology:
At present the preservation of cornea device that is adopted in the eye bank is to be with corneal film directly or insert in the blake bottle of filling culture fluid after the suspention and preserve mostly, changes liquid between storage life or does not change liquid.Its greatest problem that faces is in the system of static state, sealing, cornea and surrounding environment lack the effective substances exchange, and nutriment can not get well utilizing and replenishing in the culture fluid, and metabolite and endotoxin can't in time be discharged, restriction keratocyte eubolism shortens its holding time.And change culture fluid artificially, complicated operation can not keep the stable of cornea living environment and the pollution during effectively prevention and control are cultivated.In addition, corneal film directly contacts with blake bottle or suspends the damage that causes endothelial cell easily in midair and come off in the conventional method, in the preservation process also easily the corneal endothelial cell carry out microscopic examination.
Summary of the invention:
Main purpose of the present invention is to overcome above deficiency and a kind of save set that can effectively improve condition of culture is provided, and preserves so that be more suitable for the cultivation of cornea.Technical scheme of the present invention is: it is made up of bottle cap 7, bottle 1, dead lever 3, base 4, oral siphon 5, outlet pipe 6 and bolt 8, dead lever 3 and base 4 are fixed on the bottle 1, the height of base 4 is higher than the height of oral siphon 5 and outlet pipe 6, bolt 8 can slide up and down along dead lever 3, and bottle cap 7 covers on bottle 1 by draw-in groove 2.Its advantage is: this device can carry out from moving liquid, by continuously to the device in the perfusion cultures base can provide abundant nutrient component for cornea, and metabolite is taken away in circulation, keep metastable culture fluid concentration and pH value, sequencing simultaneously, airtight system simplification operation sequence, greatly reduce the labour and consume, reduced the pollution of external environment.Cornea is placed on the stable fulcrum of formation between bolt and the base fixedly makes cornea reduce collision with the bottle wall, is convenient to transportation and delivers.Device uses transparent material to make, and is easy to by inverted phase contrast microscope the endothelial cell in the incubation be observed.
Description of drawings:
Fig. 1 is a cross section schematic diagram of the present utility model.
Fig. 2 is the vertical view of this device.
Fig. 3, Fig. 4, Fig. 5 are the front stereograms that the cornea perfusion is cultivated each parts of save set.
1. bottles among Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5,2. draw-in groove, 3. dead lever, 4. base, 5. oral siphon, 6. outlet pipe, 7. bottle cap, 8. bolt.
Embodiment:
Open bottle cap 7, with the sclera part that aseptic microforceps is clamped corneal film, endothelium faces down and lies on the base 4, with bolt 8 gently the sclera part of fixed angles diaphragm on base 4.The peristaltic pump that starting drive is external slowly adds culture fluid the bottles 1 from oral siphon 5, treats liquid 4/5 o'clock of bottle in addition, covers tight bottle cap 7.Regulate peristaltic pump to suitable flow velocity, slowly in device, add culture fluid, utilize pressure that culture fluid unnecessary in the bottle 1 is discharged from outlet pipe 6, carry out from moving liquid.Device places 31 ℃, 5%CO
2Cultivate in the constant incubator and preserve, every day, utilization caused phase contrast microscope corneal endothelial cell to be observed.
Claims (1)
1. a cornea perfusion is cultivated save set, it is characterized in that: it is made up of bottle cap (7), bottle (1), dead lever (3), base (4), oral siphon (5), outlet pipe (6) and bolt (8), dead lever (3) and base (4) are fixed on the bottle (1), the height of base (4) is higher than the height of oral siphon (5) and outlet pipe (6), bolt (8) can slide up and down along dead lever (3), and bottle cap (7) covers on bottle (1) by draw-in groove (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200267065U CN201243574Y (en) | 2008-07-31 | 2008-07-31 | Device for cultivation and preservation of cornea with perfusion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200267065U CN201243574Y (en) | 2008-07-31 | 2008-07-31 | Device for cultivation and preservation of cornea with perfusion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201243574Y true CN201243574Y (en) | 2009-05-27 |
Family
ID=40727389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200267065U Expired - Fee Related CN201243574Y (en) | 2008-07-31 | 2008-07-31 | Device for cultivation and preservation of cornea with perfusion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201243574Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2690153C2 (en) * | 2017-06-28 | 2019-05-31 | Альвина Давидовна Мусина | Method for aseptic prolonged storage and transportation of allogenic implants, donor tissues, using an example of a donor cornea, in a special container with nanomodified surface |
| CN111787797A (en) * | 2017-12-15 | 2020-10-16 | 让·莫纳大学 | Medical device for preserving corneal tissue comprising a applanation cap and an optional trephine cap |
-
2008
- 2008-07-31 CN CNU2008200267065U patent/CN201243574Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2690153C2 (en) * | 2017-06-28 | 2019-05-31 | Альвина Давидовна Мусина | Method for aseptic prolonged storage and transportation of allogenic implants, donor tissues, using an example of a donor cornea, in a special container with nanomodified surface |
| CN111787797A (en) * | 2017-12-15 | 2020-10-16 | 让·莫纳大学 | Medical device for preserving corneal tissue comprising a applanation cap and an optional trephine cap |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090527 Termination date: 20100731 |