CN201227335Y - Biotype articular cartilage repair piece - Google Patents
Biotype articular cartilage repair piece Download PDFInfo
- Publication number
- CN201227335Y CN201227335Y CN 200820051090 CN200820051090U CN201227335Y CN 201227335 Y CN201227335 Y CN 201227335Y CN 200820051090 CN200820051090 CN 200820051090 CN 200820051090 U CN200820051090 U CN 200820051090U CN 201227335 Y CN201227335 Y CN 201227335Y
- Authority
- CN
- China
- Prior art keywords
- cartilage
- bone
- biotype
- antigens
- repair piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2/30756—Cartilage endoprostheses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2002/30001—Additional features of subject-matter classified in A61F2/28, A61F2/30 and subgroups thereof
Landscapes
- Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Cardiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
The utility model discloses a biological type joint cartilage repairing piece which takes animal joints as raw materials to crop a cartilage layer and a bone layer directly connected with the cartilage layer together to form a cartilage pile with a bone seat (see the attached picture), antigens are then removed by cross binding fixation, and a biochemistry technology such as active decorative, and the like is carried out. The main components of the cartilage layer are cartilage cell mesenchymal for removing antigens, and cartilage cell remains. The main components of the bone seat are bone matrix (ossein) for removing antigens, and inorganic components with hydroxylapatite as main materials. The antigens of the organic components of the whole repairing piece are all removed, and no exclusive reaction occurs after the repairing piece is planted. The surface of the product contains active components capable of adhering growth factors and stem cells, can induce the regeneration of cartilage and bone tissue and realize the regenerative repair of joints cartilage.
Description
Technical field
This utility model relates to medical embedded instrument field, specifically, relates to a kind of articular cartilage repair piece.
Background technology
Articular cartilage pathological changes and damage are the orthopaedics commonly encountered diseases, can cause the joint movement function obstacle, and walking pain has a strong impact on work and life, and ideal Therapeutic Method is not arranged so far.Minority Orthopeadic Surgery doctor uses from body bone～cartilage bolt (as taking from side seam outside the body patella～cartilage bolt) and makes transplantation treatment, and certain curative effect is arranged.But the Secondary cases pathological changes often takes place in the donor site, even loses more than gain, and makes us stepping back.Also have the scholar to treat, but because immune rejection problem is unresolved, the curative effect instability is added the source difficulty, problems such as ethical issues and viral cross infection, less being employed with allogeneic (corpse) bone～cartilage transplantation.Problems such as xenogenesis bone～cartilage transplantation does not reach a standard owing to treatment technology especially, and immunity rejects, animal derived virus is killed are unresolved, and are not employed.We can say that the reparation of articular cartilage damage still is in the available passive situation of a kind of no suitable material at present.
The utility model content
The purpose of this utility model provides a kind of Biotype articular cartilage repair piece, this articular cartilage repair piece is a raw material with the xenogenesis joint, after the machine-shaping, through take off cell, crosslinked fixing, remove biochemical treatments such as antigen and surface activity modification and make, it is a column that the bone seat (2) that is connected with cartilage by cartilage layers (1) is formed.The main composition of cartilage layers (1) is a matter between antigenic chondrocyte, and it comprises the extracellular matrix and the collagen fiber that are hidden in wherein of cartilage.The main composition of bone seat (2) is that the antigen bone matrix reaches the inorganic composition based on hydroxyapatite.Surface (comprising surfaces externally and internally) at goods also is provided with an active material layer, can adhere to somatomedin and stem cell, induces the regeneration of bone and cartilage.
Compare with existing product, the utlity model has following beneficial effect:
Biotype articular cartilage repair piece of the present utility model, joint of animal such as pig, cattle is a raw material to originate widely, make through removing the modification of antigen and surface activity effectively, the organic and inorganic composition and the basic structure that have kept cartilage and bone, kept chondro-osseous binding pattern, good biocompatibility, after being used for repairing implantation, do not cause repulsion, can collect somatomedin and mesenchymal stem cells MSCs that body self-regeneration mechanism discharges, induce the regeneration of osseous tissue and cartilaginous tissue, finally realize the reproducibility reparation.
Description of drawings
Fig. 1 is the structural representation of cylindric Biotype articular cartilage repair piece;
Fig. 2 is the structural representation of flat column Biotype articular cartilage repair piece;
1 is cartilage layers, and 2 is the bone seat that is connected with cartilage.
The specific embodiment
Gather the fresh healthy swine joint, put in 0.1% the bromo geramine solution, soaking disinfection 30 minutes takes out, and cuts off the fork ligament, removes impurity, cuts the cartilage of subzonal layer bone with specific purpose tool, makes into column, and the upper strata of post is cartilage layers (1), and hypomere is bone seat (2).It has kept basic structure and basic composition that cartilage is connected with the substrate bone.Put into high infiltration and take off cell reactor, added the enzymolysis solution enzymolysis 8 hours, 37 ℃ of temperature.Enzymolysis solution pepsin-tryptic multiple enzyme.With the enzyme eluting and make its inactivation, put into high infiltration fixed reactor after enzymolysis is finished, add fixative, 36 hours response time.Fixative is the liquid of ring-containing oxide 0.1mol/L, and epoxide is
, R=(CH
3)
3CCH
2-.Take out after fixedly finishing, in and epoxide, clean.Put into high infiltration and remove the antigen-reactive device, add respectively and remove antigen reagent, 20 ℃ of reactions 8 hours, used to remove antigen reagent be anhydride and guanidine hydrochloride, successively reacts except that antigen reagent with two kinds, with abundant removal antigenicity.Take out, clean.Put into high infiltration and organize induced reaction device, adding can adhere to somatomedin and cell activity substance solution and coupling agent solution, make reaction 8 hours, 10 ℃ of reaction temperatures.The polypeptide of active substance for relying (16)-Gan-essence-Gan-Radix Asparagi-Si-dried meat-half Guang to form, coupling agent is dissident's dicarboxylic anhydride.Reaction is finished the back and is taken out, and cleans, and encapsulates, and send irradiation central gamma-ray (25KGy) virus of going out, and gets product.
Cut and derive from cattle, be the Biotype articular cartilage repair piece of flat column, as shown in Figure 2, other is with embodiment 1.
Claims (4)
1. Biotype articular cartilage repair piece is characterized in that it is the cartilage that cuts band bone seat from the joint of animal, be one comprise cartilage layers (1) and with the column of the direct banded bone seat of cartilage (2).
2. Biotype articular cartilage repair piece according to claim 1 is characterized in that its cartilage layers (1) by going between antigenic chondrocyte matter to form, and matter comprises cartilage matrix and the collagen fiber that are hidden in the substrate are formed between this chondrocyte.
3. Biotype articular cartilage repair piece according to claim 1 is characterized in that its bone seat (2) is by removing antigenic bone matrix, mainly is that ossein reaches the inorganic component composition based on hydroxyapatite.
4. Biotype articular cartilage repair piece according to claim 1 is characterized in that its surface, comprises that internal pore surface is provided with the active material layer that one deck can adhere to growth-factor-enriched and stem cell, and this active substance is specific polypeptide, or glycosaminoglycans.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820051090 CN201227335Y (en) | 2008-07-22 | 2008-07-22 | Biotype articular cartilage repair piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820051090 CN201227335Y (en) | 2008-07-22 | 2008-07-22 | Biotype articular cartilage repair piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201227335Y true CN201227335Y (en) | 2009-04-29 |
Family
ID=40632295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200820051090 Expired - Lifetime CN201227335Y (en) | 2008-07-22 | 2008-07-22 | Biotype articular cartilage repair piece |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201227335Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102151155A (en) * | 2011-04-26 | 2011-08-17 | 卫小春 | Tool and method for establishing experimental model for researching influence of environments in articular cavity and under cartilage to articular cartilage |
-
2008
- 2008-07-22 CN CN 200820051090 patent/CN201227335Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102151155A (en) * | 2011-04-26 | 2011-08-17 | 卫小春 | Tool and method for establishing experimental model for researching influence of environments in articular cavity and under cartilage to articular cartilage |
| CN102151155B (en) * | 2011-04-26 | 2013-02-27 | 卫小春 | Tool and method for establishing experimental model for researching influence of environments in articular cavity and under cartilage to articular cartilage |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101332314B (en) | Biotype articular cartilage repair piece | |
| KR101424308B1 (en) | In situ system for intra-articular chondral and osseous tissue repair | |
| WO2006093151A1 (en) | Cultured cell sheet, production method thereof, and application method thereof | |
| EP3331984B1 (en) | Rapid allograft treatment systems and methods | |
| CN1545549A (en) | Isolation of inner cell mass for the establishment of human embryonic stem cell (hESC) lines | |
| CN103785065B (en) | Preparation method of cartilage regeneration scaffold material | |
| CN101537207B (en) | Preparation method of tissue engineering xenoskin | |
| CN109789246A (en) | Regenerating bone or cartilage composition and its manufacturing method | |
| CN104011201A (en) | Fibroblasts For Treatment Of Degenerative Disc Disease | |
| WO2008006544A3 (en) | Use of gelatin and a cross-linking agent for producing a cross-linking therapeutic composition | |
| TWI450735B (en) | Composition for repairing cartilage tissue and method for making the same | |
| CN101417151A (en) | Biology source type calcified-layer containing cartilage tissue engineer bracket | |
| CN104307044B (en) | A kind of preparation method of the de-cell material of total spinal disc in natural tissues source | |
| RU2682159C2 (en) | Fgf-18 in transplantation and tissue engineering procedures | |
| CN1297658C (en) | Tissue engineering cartilage construction method using bone matrix gelatin | |
| CN105534619A (en) | Cartilage repair and compensation prosthesis | |
| Zhao et al. | In vivo investigation of tissue-engineered periosteum for the repair of allogeneic critical size bone defects in rabbits | |
| CN101332316A (en) | Biotype nose bridge implantation body | |
| CN201227335Y (en) | Biotype articular cartilage repair piece | |
| WO2004011631A3 (en) | Methods and compositions for treating tissue defects using pulsed electromagnetic field stimulus | |
| KR20200012954A (en) | Cartilage Graft Scaffold | |
| RU2545993C2 (en) | Method for repairing long bone defects of critical size | |
| CN102871774B (en) | Ligament repair stent and preparation method and application thereof | |
| RU86455U1 (en) | BIO ENGINEERING DESIGN | |
| CN201182778Y (en) | Biological type bone repair material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: GUANGDONG GUAN HAO BIOLOGICAL SCIENCE + TECHNOLOGY Free format text: FORMER OWNER: CANTON ZHIGUANG BIOTECHNOLOGY CO., LTD. Effective date: 20090612 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20090612 Address after: D, Guangzhou international business incubator, Guangzhou Science City, Guangdong, Guangzhou 408, China: 510663 Patentee after: Guangdong summit life sciences Co., Ltd. Address before: Room 2204, east ring building, 474 Ring Road, Guangdong, Guangzhou Province, China: 510075 Patentee before: Zhiguang Biological Sci-Tech Co., Ltd., Guangzhou |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090429 |