CN201031226Y - Iron bacteria simple testing bottle - Google Patents
Iron bacteria simple testing bottle Download PDFInfo
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- CN201031226Y CN201031226Y CNU2006201348677U CN200620134867U CN201031226Y CN 201031226 Y CN201031226 Y CN 201031226Y CN U2006201348677 U CNU2006201348677 U CN U2006201348677U CN 200620134867 U CN200620134867 U CN 200620134867U CN 201031226 Y CN201031226 Y CN 201031226Y
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- iron bacteria
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- test
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Abstract
The utility model relates to a test controller for testing water and microorganisms in the sludge deposits, in particular to a testing bottle for detecting water or iron bacteria in other substances. The utility model comprises a sealed colorless transparent bottle, the testing liquid of the iron bacteria is filled in the bottle, the ratio of the height H1 of the testing liquid in the testing bottle to the gap height H2 of the uniform section of the testing bottle is shown like H1:H2=1:0.3-0.8, the internal portion of the testing bottle is taken in the aseptic state before testing, and the testing liquid of the iron bacteria contains 0.05-0.3% of the sulfate, 0.01-0.1% of the chloride, 0.5-1.5% of the ferri-ammonium salt, 0.03-0.1% of the phosphate, 0.01-0.1% of the nitrate, and 97.9-99.4% of the distilled water. The utility model can be used for qualitatively and quantitatively detecting the iron bacteria in the substances such as water and sludge deposits. The utility model has the advantages of convenient carrying and utilization, simply quick and accurate testing, and long retention time.
Description
Technical field
The utility model relates to the Testing apparatus of microorganism in a kind of water and the sludge sediment, particularly relates to the test bottle of iron bacteria in a kind of water or other material.
Background technology
Iron bacteria extensively is present in the water and various confessions, drainage pipeline in, its growth metabolism and breeding easily cause the erosion of metallic substance, destructive deterioration, rotten causes pipeline and ships shell etc. to produce corrosion, causes serious economy loss.
The mensuration of iron bacteria does not still have national standard method at present, traditional measuring method is the multitube fermentation method, its general schedule of operation is: 1. gather the medium preparation of the vessel of the sterilized water of water sample → 2. preparation → 3., reagent sterilization → 4. → 5. dilute and inoculate the microbial culture of water sample → 6. → 7. counting and report, finishing whole test process generally need 10 day.Multitube fermentation method not only schedule of operation is loaded down with trivial details, complicated, time is tediously long, and its competency profiling to test environment and operator is high, from Sampling techniques, laboratory condition, plant and instrument, vessel reagent quality, operators'skills, all observation processes and sequence of control all have strict regulation and requirement until quality control etc.In addition, numerous and diverse test link very easily produces bigger mistake. and poor, cause test result inaccurate.
The utility model content
The purpose of this utility model provide a kind of test fluid that detects iron bacteria in water or other material and. the easily-testing bottle.
The technical scheme that the utility model technical solution problem is adopted is:
Principle according to iron bacteria meta-bolites and particular agent generation colour developing and chemical precipitation reaction is prepared the highly selective liquid nutrient medium, seals up for safekeeping in clear vial, and sterilization, thereby make storable simple and easy iron bacteria test bottle.
A kind of iron bacteria easily-testing bottle comprises the iron bacteria test fluid of being adorned in the water white transparency bottle that seals and the bottle, the height H of test fluid in test bottle
1With the test bottle uniform cross section height H of leaving a blank
2Ratio H
1: H
2=1: 0.3～0.8, described test bottle inside is sterile state before test.
Described water white transparency bottle is the colourless transparent glass bottle that seals with plug and aluminium cover.
Described iron bacteria test fluid contains vitriol 0.05～0.3%, muriate 0.01～0.1%, ferric ammonium 0.5～1.5%, phosphoric acid salt 0.03～0.1%, nitrate 0.01～0.1%, distilled water 97.9～99.4%.
Vitriol in the described iron bacteria test fluid can be in sal epsom, the ammonium sulfate one or both.
Muriate in the described iron bacteria test fluid can be in calcium chloride, the Repone K one or both.。
Ferric ammonium in the described iron bacteria test fluid can be ferric ammonium citrate salt.
Phosphoric acid salt in the described iron bacteria test fluid can be in potassium primary phosphate, the dipotassium hydrogen phosphate one or both.
Nitrate in the described iron bacteria test fluid can be in SODIUMNITRATE, the saltpetre one or both.
Described iron bacteria test fluid contains sal epsom 0.05～0.3%, calcium chloride 0.01～0.1%, ferric ammonium citrate 0.5～1.5%, dipotassium hydrogen phosphate 0.03～0.1%, SODIUMNITRATE 0.01～0.1%, distilled water 97.9～99.4%.
The utlity model has following actively useful effect:
(1) sealing of special substratum test fluid is stored in the vial, and sterilization, and storage period is longer, saves that the multitube fermentation method is loaded down with trivial details, the complicated operations step, and only need 7 days detection time, shortened test greatly. the time, alleviated labour intensity.
(2) do not need the desired aseptic technique of traditional method, make biochemical investigation in general environment, to carry out, and then save a large amount of infrastructure investments.
(3) characteristic reaction of iron bacteria and test fluid is to carry out automatically in aseptic bacterium test bottle, can avoid contamination and interference in the rapid operating process of multistep of traditional method, detected result accurately and reliably, and minimum detectability meets national water quality standard requirement.
(4) can be applicable to the simple and easy quick test of iron bacteria in various water quality, mud and the settling, can learn operation in non-specialised staff's number minute.
Description of drawings
Fig. 1 is a kind of structural representation of iron bacteria easily-testing bottle.
Embodiment
Embodiment one: referring to Fig. 1,1 is aluminium cover among the figure, and 2 is plug, 3 uniform cross section vial chambeies, and 4 is the iron bacteria test fluid.H
1Be the height of iron bacteria test fluid, H
2For the test bottle uniform cross section is left a blank highly.Test bottle is the water white transparency oral liquid bottle of 15mL in the present embodiment, the height H of test fluid
1Be 6cm, the height H of leaving a blank
2Be 4cm.
Above-mentioned test fluid prescription is: sal epsom 0.05%, calcium chloride 0.01%, ferric ammonium citrate 0.5%, dipotassium hydrogen phosphate 0.03%, SODIUMNITRATE 0.01%, distilled water 97.9%.After test fluid prepares, be packaged in by every bottle of 9.0mL in the water white transparency oral liquid bottle of 15mL, autoclaving got final product in 15 minutes.
To draw the 1.0mL water sample with asepsis injector during use, be injected in the test bottle, be positioned over after shaking up in 37 ℃ of loft drier or the water-bath 24 hours, (pale brown look takes off during the positive reaction of iron bacteria bottle to judge feminine gender or positive reaction according to the variation of liquid in the bottle, have black or brown precipitate to generate), this is qualitative analysis; According to said method do 2 groups or 3 groups of parallel samples, look into the MPN table, can carry out quantitative test by the stepwise dilution method.Other solid sample such as mud then must be with water dissolution or lixiviate, is processed into behind the liquid again by above-mentioned.Method is measured.
Embodiment two: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 8mL, the height H of test fluid
1Be 4.5cm, the height H of leaving a blank
2Be 3.5cm.
The test fluid prescription is: sal epsom 0.3%, calcium chloride 0.1%, ferric ammonium citrate 1.5%, dipotassium hydrogen phosphate 0.1%, SODIUMNITRATE 0.1%, surplus are distilled water.After above-mentioned test solution prepared, be packaged in by every bottle of 4.5mL in the water white transparency cillin bottle of 8mL, autoclaving got final product in 15 minutes.
To draw 0.5 ml water quality sample with asepsis injector during use, and be injected in the test bottle, other step is identical with embodiment one.
Embodiment three: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency round bottom vial of 10mL, the height H of test fluid
1Be 3.0cm, the height H of leaving a blank
2Be 0.9cm.
Above-mentioned test fluid prescription is: sal epsom 0.1%, calcium chloride 0.02%, ferric ammonium citrate 1.0%, dipotassium hydrogen phosphate 0.05%, SODIUMNITRATE 0.05%, surplus are distilled water.After above-mentioned test solution prepared, be packaged in the 10mL test bottle by 4.5 milliliters every bottle, autoclaving got final product in 15 minutes.
Using method is identical with embodiment two, does not repeat.
Embodiment four: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 8.1mL, the height H of test fluid
1Be 3.0cm, the height H of leaving a blank
2Be 2.4cm.
Above-mentioned test fluid prescription is: ammonium sulfate 0.2%, calcium chloride 0.05%, ferric ammonium citrate 0.8%, potassium primary phosphate 0.06%, SODIUMNITRATE 0.08%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Adopt the disappearance dilution method during use, promptly (solid sample then must be with water dissolution or lixiviate with water sample to be determined with asepsis injector, measure after making liquid to be measured) be injected into step by step in the test bottle, after inoculating dilution, place incubator to cultivate, according to the multiple of test bottle positive reaction and dilution, calculate amount of bacteria sum in the water sample.Pale brown look takes off during the positive reaction of iron bacteria bottle, has black or brown precipitate to generate
Embodiment five: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 12.6mL, the height H of test fluid
1Be 5.0cm, the height H of leaving a blank
2Be 2.0cm.
Above-mentioned test fluid prescription is: ammonium sulfate 0.05%, Repone K 0.01%, ferric ammonium citrate 0.5%, potassium primary phosphate 0.03%, saltpetre 0.01%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Using method is identical with embodiment four.
Embodiment six: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 6.5mL, the height H of test fluid
1Be 4.5cm, the height H of leaving a blank
2Be 1.6cm.
Above-mentioned test fluid prescription is: ammonium sulfate 0.3%, Repone K 0.1%, ferric ammonium citrate 1.5%, phosphoric acid. potassium dihydrogen 0.1%, saltpetre 0.1%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Using method is identical with embodiment four.
Embodiment seven: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 6mL, the height H of test fluid
1Be 5.0cm, the height H of leaving a blank
2Be 2.5cm.
Above-mentioned test fluid prescription is: ammonium sulfate 0.1%, Repone K 0.02%, ferric ammonium citrate 1.0%, potassium primary phosphate 0.05%, saltpetre 0.05%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Using method is identical with embodiment four.
Embodiment eight: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 5.0mL, the height H of test fluid
1Be 4.5cm, the height H of leaving a blank
2Be 2.0cm.
Above-mentioned test fluid prescription is: sal epsom 0.2%, sodium-chlor 0.05%, ferric ammonium citrate 0.5%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.5%, saltpetre 0.05%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Using method is identical with embodiment four.
Embodiment nine: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 6.0mL., the height H of test fluid
1Be 5.0cm, the height H of leaving a blank
2Be 3.0cm.
Above-mentioned test fluid prescription is: sal epsom 0.1%, ammonium sulfate 0.2%, sodium-chlor 0.05%, Repone K 0.05%, ferric ammonium citrate 0.5%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.3%, SODIUMNITRATE 0.05%, saltpetre 0.02%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Using method is identical with embodiment four.
Embodiment ten: present embodiment and embodiment one are basic identical, and difference is: test bottle is the water white transparency cillin bottle of 6.0mL., the height H of test fluid
1Be 5.0cm, the height H of leaving a blank
2Be 2.0cm.
Above-mentioned test fluid prescription is: sal epsom 0.3%, sodium-chlor 0.03%, Repone K 0.05%, ferric ammonium citrate 0.6%, dipotassium hydrogen phosphate 0.4%, potassium primary phosphate 0.3%, SODIUMNITRATE 0.05%, surplus are distilled water.After above-mentioned test solution prepared, measuring tank was contained in the above-mentioned test bottle as required, and autoclaving got final product in 15 minutes.
Using method is identical with embodiment four.
Claims (8)
1. an iron bacteria easily-testing bottle comprises the water white transparency bottle that seals, and it is characterized in that: described iron bacteria test fluid is housed, the height H of test fluid in test bottle in the water white transparency bottle
1With the test bottle uniform cross section height H of leaving a blank
2Ratio H
1: H
2=1: 0.3～0.8.
2. iron bacteria easily-testing bottle according to claim 1 is characterized in that: described water white transparency bottle is the colourless transparent glass bottle that seals with plug and aluminium cover.
3. iron bacteria easily-testing bottle according to claim 1 and 2 is characterized in that: described iron bacteria test fluid contains vitriol, muriate, ferric ammonium, phosphoric acid salt, nitrate, distilled water, mixes by a certain percentage.
4. iron bacteria easily-testing bottle according to claim 3 is characterized in that: described vitriol is one or both in sal epsom, the ammonium sulfate.
5. iron bacteria easily-testing bottle according to claim 4 is characterized in that: described muriate is one or both in calcium chloride, the Repone K.
6. iron bacteria easily-testing bottle according to claim 5 is characterized in that: described ferric ammonium is the ironic citrate ammonium salt.
7. iron bacteria easily-testing bottle according to claim 6 is characterized in that: described phosphoric acid salt is one or both in potassium primary phosphate, the dipotassium hydrogen phosphate.
8. iron bacteria easily-testing bottle according to claim 7 is characterized in that: described nitric acid hydrochlorate is one or both in SODIUMNITRATE, the saltpetre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2006201348677U CN201031226Y (en) | 2006-11-30 | 2006-11-30 | Iron bacteria simple testing bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2006201348677U CN201031226Y (en) | 2006-11-30 | 2006-11-30 | Iron bacteria simple testing bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201031226Y true CN201031226Y (en) | 2008-03-05 |
Family
ID=39163120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2006201348677U Expired - Fee Related CN201031226Y (en) | 2006-11-30 | 2006-11-30 | Iron bacteria simple testing bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201031226Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101029330B (en) * | 2006-11-30 | 2010-10-06 | 白莉 | Iron bacterium testing fluid and simple testing tube |
-
2006
- 2006-11-30 CN CNU2006201348677U patent/CN201031226Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101029330B (en) * | 2006-11-30 | 2010-10-06 | 白莉 | Iron bacterium testing fluid and simple testing tube |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080305 Termination date: 20101130 |