CN201020355Y - Gradient mixing device - Google Patents
Gradient mixing device Download PDFInfo
- Publication number
- CN201020355Y CN201020355Y CNU2007200627973U CN200720062797U CN201020355Y CN 201020355 Y CN201020355 Y CN 201020355Y CN U2007200627973 U CNU2007200627973 U CN U2007200627973U CN 200720062797 U CN200720062797 U CN 200720062797U CN 201020355 Y CN201020355 Y CN 201020355Y
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- cup
- gradient
- passage
- mixing device
- gradient mixing
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Abstract
The utility model relates to a gradient mixing device for casting the polyacrylamide gradient gelatin in lab. The main part of the device is an organic glass mixer cup body, which is composed of a cup base (1) with supporting feet (2), and two cup chambers - a Cup A (3) and a Cup B (4). The supporting feet (2) functions as a support and also helps to realize the regulation of partial height of the gradient mixing cup; the passage (8) between the two cups is provided with vascular clamps (5); the passage (8) between the two cups adopts the one way liquid delivery tube and is easy to wash and convenient to exchange; the cup base (1) is provided with a bar gradienter (6), which is used for checking whether the cup base is horizontal before operation; a magnetic stirring rod (7) is positioned in the Cup B (4); the peristaltic pump (9) is used for controlling the mixing speed and pumping the mixed gradient gelatin to the glue plate (10). The entire producing process is simple and achieves a good effect.
Description
Technical field
The utility model relates to a kind of mixer apparatus that is used to record gradient gel, and its this function is to realize by a combination of overlapping organic glass elements.
Background technology
Gradient gel electrophoresis also adopts polyacrylamide gel usually, but is not to carry out on the gel in single concentration (aperture), but forms gradient gel.Concentration in gradient from the gel top to the bottom acrylamide changes, and is 5% as common top gel strength, and the bottom gel strength is 25%.Gel gradient forms by gradient mix device, and the acrylamide solution of high concentration at first joins in the glass plate, and then solution concentration descends in gradient, and is therefore bigger in the aperture, top of gel, and less at the bottom part aperture diameter of gel.Gradient gel electrophoresis also adds SDS usually, and concentrated glue is arranged.Electrophoresis process and SDS-polyacrylamide gel electrophoresis are similar substantially.
With the gel phase ratio of single concentration, gradient gel has several advantages: 1. at first, gradient gel is wideer than the separating ranges of single concentration gel, can separate the protein of molecular weight in a big way simultaneously.2. another advantage is that gradient gel can be differentiated molecular weight and differs less, unresolvable protein in single concentration gel.3. can directly measure the molecular weight of native state protein and not need to be dissociated into subunit, so this method can complement one another with the method for SDS-PAGE determining molecular weight.
The mixer cup of general commercially available gradient mix device is made up of two cup chambeies, cup and support foot.Cup is the lucite flat board, and interior have two control valves in addition by two cup chambeies of passage connection and outlet, and cup is divided into four and supported foot, supports foot and only plays a supportive role, and can not regulate highly.Because using gradient mix device has relatively high expectations to the cup horizontality, when particularly using magnetic agitation, if the gradient mixer cup is out-of-level, not only easily cause magnetic bar hold to stagnate, and the output gradient of mixed liquor will change, cause the test poor repeatability, influence result of the test, therefore before gradient mix device carries out the wash-out operation or joins gradient gel, need carry out horizontal adjusting because it is fixing to support foot, can only back cushion paper etc. method realize the adjustment of gradient mixer cup level.In addition, passage places in the cup between commercially available gradient mix device cup, easily causes gelling solid because time assurance is bad when joining gradient gel, thereby stops up passage between cup, cleans unusually difficult.And the specification of producer is limited after all, for specific mixed volume, and the too big or too little effect that all will influence the gradient generation of gradient former container.
The utility model content
The purpose of this utility model is to clean deficiencies such as difficulty at passage between existing gradient mix device cup adjusting level inconvenience, cup, provides a cover to regulate that passage is easy to clean and change gradient mixing device easily between cup level, cup by supporting foot.
Main part of the present utility model is a lucite blender cup, is supported cup (1), A cup (3) and (4) two cup chambeies of B cup of foot (2) by band and forms; Support foot (2) by be installed in cup (1) all around the screw on the screw constitute; Passage (8) is linked to be an integral body by connecting bottom, cup chamber aperture with A cup (3) and B cup (4) between cup, and passage (8) adopts one-time infusion tube between cup, has blood vessel clip (5) on the pipe; Be fixed with strip level meter (6) on the cup (1), be used to check whether level of cup before the operation; B cup (4) is built-in with magnetic stirring bar (7); Peristaltic pump (9) be used to control mixing velocity and with mixed gradient gel pump to glue plate (10).
The foot that supports of the present utility model had not only played a supportive role but also can realize that adjusting to gradient mix device cup local height was to be up to the standard; Passage adopts one-time infusion tube between cup, and it is convenient to be easy to clean and to change; The utlity model has simple structure, easy to operate, plurality of advantages such as result of use is good.
Description of drawings
Accompanying drawing 1 is a gradient mix device schematic diagram of the present utility model
In the accompanying drawing: 1, cup; 2, support foot; 3, A cup; 4, B cup; 5, blood vessel clip; 6, strip level meter; 7, magnetic stirring bar; 8, passage between the cup; 9, peristaltic pump; 10, glue plate
Specific embodiments
Main part of the present utility model is a lucite blender cup, is supported cup (1), A cup (3) and (4) two cup chambeies of B cup of foot (2) by band and forms; Support foot (2) by be installed in cup (1) all around the screw on the screw constitute; Adopt one-time infusion tube as passage between cup (8), A cup (3) and B cup (4) are linked to be an integral body, have blood vessel clip (5) on the passage (8) between cup; Be fixed with strip level meter (6) on the cup (1), be used to check whether level of cup before the operation; B cup (4) is built-in with magnetic stirring bar (7); Peristaltic pump (9) be used to control mixing velocity and with mixed gradient gel pump to glue plate (10).
Pass through four of adjustings before the operation earlier and support the level that the height of foot (2) is realized the gradient mix device cup, clamp between cup with blood vessel clip (5) and in A cup (3) and B cup (4), to pour rare sol solution and the dense sol solution for preparing behind the passage simultaneously respectively into, open peristaltic pump (9) when unclamping blood vessel clip (5), mixed glue will be by peristaltic pump (9) pump to glue plate (10).
Claims (3)
1. a gradient mixing device is characterized in that its main part is a lucite blender cup, is supported cup (1), A cup (3) and (4) two cup chambeies of B cup of foot (2) by band and forms; Support foot (2) by be installed in cup (1) all around the screw on the screw constitute; Passage (8) is linked to be an integral body by connecting bottom, cup chamber aperture with A cup (3) and B cup (4) between cup, has blood vessel clip (5) on the passage (8) between cup; Be fixed with strip level meter (6) on the cup (1); B cup (4) is built-in with magnetic stirring bar (7).
2. gradient mixing device according to claim 1 is characterized in that passage between cup (8) adopts one-time infusion tube.
3. gradient mixing device according to claim 1 is characterized in that gradient mixing device also comprises peristaltic pump (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200627973U CN201020355Y (en) | 2007-03-28 | 2007-03-28 | Gradient mixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200627973U CN201020355Y (en) | 2007-03-28 | 2007-03-28 | Gradient mixing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201020355Y true CN201020355Y (en) | 2008-02-13 |
Family
ID=39087761
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200627973U Expired - Fee Related CN201020355Y (en) | 2007-03-28 | 2007-03-28 | Gradient mixing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201020355Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716685A (en) * | 2012-07-17 | 2012-10-10 | 上海禾高生物科技有限公司 | Continuous gradient mixing device and mixing method thereof |
| CN105854723A (en) * | 2016-06-08 | 2016-08-17 | 浙江大学 | Coupling pump type convenient and fast gradient mixer |
| CN105903397A (en) * | 2016-06-08 | 2016-08-31 | 浙江大学 | Dual-pump type gradient mixer convenient and fast to use |
| CN107128864A (en) * | 2017-03-21 | 2017-09-05 | 上海从生生物科技有限公司 | The Automatic Control glue pouring system and method continuously made for gradient gel film |
| CN107638590A (en) * | 2017-09-20 | 2018-01-30 | 大连理工大学 | A kind of bionical compound support frame material of chitosan-based gradient and its construction method |
-
2007
- 2007-03-28 CN CNU2007200627973U patent/CN201020355Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716685A (en) * | 2012-07-17 | 2012-10-10 | 上海禾高生物科技有限公司 | Continuous gradient mixing device and mixing method thereof |
| CN102716685B (en) * | 2012-07-17 | 2014-08-06 | 上海禾高生物科技有限公司 | Continuous gradient mixing device and mixing method thereof |
| CN105854723A (en) * | 2016-06-08 | 2016-08-17 | 浙江大学 | Coupling pump type convenient and fast gradient mixer |
| CN105903397A (en) * | 2016-06-08 | 2016-08-31 | 浙江大学 | Dual-pump type gradient mixer convenient and fast to use |
| CN107128864A (en) * | 2017-03-21 | 2017-09-05 | 上海从生生物科技有限公司 | The Automatic Control glue pouring system and method continuously made for gradient gel film |
| CN107638590A (en) * | 2017-09-20 | 2018-01-30 | 大连理工大学 | A kind of bionical compound support frame material of chitosan-based gradient and its construction method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080213 Termination date: 20140328 |