CN200996959Y - External analog mineralizer of intelligent multi-parameter - Google Patents
External analog mineralizer of intelligent multi-parameter Download PDFInfo
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- CN200996959Y CN200996959Y CN 200720047892 CN200720047892U CN200996959Y CN 200996959 Y CN200996959 Y CN 200996959Y CN 200720047892 CN200720047892 CN 200720047892 CN 200720047892 U CN200720047892 U CN 200720047892U CN 200996959 Y CN200996959 Y CN 200996959Y
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- concentration adjustment
- peristaltic pump
- placement unit
- chamber
- electrode
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Abstract
An intelligent mineralization simulating device with the multi-parameter is provided; which is characterized in that the device comprises a concentration control room, a mineralization room, and a specimen placement unit. The concentration control room is connected with the mineralization room via the pipe. A peristaltic pump is available in the middle of the pipe; the mineralization room is connected with the specimen placement unit via the pipe. Also, a peristaltic pump is available in the middle of the pipe; the specimen placement unit is connected with the concentration control room via the pipe; driven by the peristaltic pump, the simulated body fluid realizes the circulation flow among the concentration control room, the mineralization room and the specimen placement unit; the concentration control room, the mineralization room and the specimen placement unit are all placed inside the water basin. Also, the water basin is equipped with a heater. The concentration control room is connected with a couple of the ion replenishing pools via the pipe and the multi-channel micro-injection pump; a signal detection system is available, such single detection system comprises an analyzer, a Ca ion electrode, a pH electrode and a temperature detector. Both the Ca ion electrode and the pH electrode are equipped in the concentration control room; the temperature detector is arranged inside the water basin; the Ca ion electrode, the pH electrode and the temperature detector are all electrically connected with the analyzer; the peristaltic pump, the heater, the multi-channel micro-injection pump and the analyzer are all connected with the PC. The PC is installed with the control software so as to control the operation of the peristaltic pump, the heater, the multi-channel micro-injection pump and the analyzer. Thus, physiological environment inside the human body could be simulated.
Description
Technical field
The utility model relates to bio-medical material evaluation and bionical preparation system technical field, specifically is meant the intelligent type multi-parameter external mineralizing analogue means that is used for biomedicine and Tissue Engineering Study.
Background technology
In physiological environment, the bone like apatite layer of bioactive materials surface/interface deposition plays a crucial role to the formation of bone bonding, there are some researches show: the osteoid apatite of material surface, the molecular recognition of albumen and cell and selective adsorption/stick there is important effect, and help cell in its surface propagation, division and growth, new bone can directly form on bone like apatite layer, and combines with bone like apatite layer formation chemical.Thereby can Available Material surface/interface bone like apatite layer form and form biologically active and new ostosis ability that the speed speed is judged artificial bone, and understand skeletonization mechanism on the bioactive materials in depth by the formation mechanism of research bone like apatite layer.
In-vitro simulated body fluid infusion method is bioenvironmental a kind of in vitro test method commonly used in the analogue body, also is the basic skills that is used for studying biological surface of active material bone like apatite layer formation and skeletonization mechanism at present.Most data results that this external mineralising method obtains can be implanted into test findings by better prediction material bone, thereby become a kind of the recognized standard method.
The immersion process of research has static immersing method and dynamic soaking method at present.The static immersing method is at present domestic and international general a kind of method, the material that this method is handled is implanted at bone and has obtained than ten-strike, biologically active and the osteogenic ability of can the apatite layer by external formation investigating material, but the implanting result appearance in the test result of this method and muscle or the non-bone tissue such as subcutaneous is than big-difference.One of reason that it is possible be since in the SBF solution part ion concentration and its concentration in blood plasma have than big difference; Its two, the flow state of simulated body fluid and the mode and the body that flow through sample have been implanted into than big difference; Its three, under in-vitro simulated state, the calcium and phosphorus ions concentration difference of diverse location is bigger in material surface calcium and phosphorus ions concentration in the simulated body fluid and the solution, and under the effect of body fluid, differs bigger in material surface calcium and phosphorus ions concentration and the body fluid.Therefore, there are bigger difference in the apatite layer that obtains of static simulation and bone surface apatite layer.Also have at present and adopt the dynamic soaking method to simulate the report of mineralising research, but the device that adopted of all dynamic soaking methods only can interior one or both parameters of parody at present.In addition, the forming process of in-vitro simulated biomineralization layer (bone like apatite layer) must be considered the effect that organic substance such as the contained albumen of body fluid forms mineral matter, and above simulation system has all been ignored the influence that complicated organic principle forms bone like apatite layer in the body fluid, and the flow state of simulated body fluid and the modes of emplacement of sample and body have been implanted into bigger difference, and therefore the apatite mineralized layer that is obtained not is proper biomineralization layer.
The utility model content
The purpose of this utility model is at the deficiencies in the prior art part, a kind of intelligent type multi-parameter external mineralizing analogue means is provided, this device adopts digital processing technology, intelligence system technology and detection and feedback control technology, use simple to operate, testing result is reliable, can be under lower operating cost to body in multiparameter accurately reproduce, the activity rating that carries out material for the bio-medical material research field provides reliable platform.
Intelligent type multi-parameter external mineralizing analogue means of the present utility model comprises concentration adjustment chamber, ageing chamber, sample placement unit; Described concentration adjustment chamber links to each other with the ageing chamber by pipeline, and a peristaltic pump is set in the middle of the described pipeline; Described ageing chamber links to each other with the sample placement unit by pipeline, and a peristaltic pump also is set in the middle of the described pipeline; Described sample placement unit links to each other with the concentration adjustment chamber by pipeline; Under the effect of peristaltic pump, simulated body fluid circulates in concentration adjustment chamber, ageing chamber, sample placement unit;
Described concentration adjustment chamber, ageing chamber, sample placement unit all are arranged in the water bath, and a well heater also is set in the described water bath;
Described concentration adjustment chamber replenishes the pond by pipeline with hyperchannel micro-injection pump and several ions and links to each other;
Described device also comprises a signal detection system, described signal detection system comprises that an analyser, one are arranged at the Ca ion electrode of concentration adjustment chamber, a pH electrode that is arranged at the concentration adjustment chamber, a Temperature Detector that is arranged in the water bath, and described Ca ion electrode, pH electrode and Temperature Detector all are electrically connected with analyser;
Described peristaltic pump, well heater, hyperchannel micro-injection pump and analyser all are electrically connected with PC, Control Software is housed in the PC, by the action of PC control peristaltic pump, well heater, hyperchannel micro-injection pump and analyser, realize the simulation reconstruction of Human Physiology environment.
In order to make the Temperature Distribution in the water bath more even, a stirrer is set in water bath, described stirrer is electrically connected with PC, by the action of PC control stirrer.
For the Temperature Distribution and the ion distribution that make the concentration adjustment chamber is more even, in the concentration adjustment chamber stirrer is set, described stirrer is electrically connected with PC, by the action of PC control stirrer.
For the better temperature that must reflect simulated body fluid, in the concentration adjustment chamber Temperature Detector is set, described Temperature Detector is electrically connected with analyser.
In order to make device be adapted to the body fluid situation of various complexity, temperature, flow velocity, Ca ion concentration and pH value can be set in the described Control Software, make simulated body fluid reach setting value by each parts of accurate control.
In order better to realize the utility model, temperature, flow velocity, flow, Ca ion concentration and pH value that described Control Software can the real time record simulated body fluid are so that analyze data better.
Be provided with in order to find that in time operating fault, described Control Software have to report to the police, the various faults that occur during to the device operation can in time be reported to the police.
The utility model compared with prior art, have following advantage: the utility model is by each parameter of software control system setting device, adopt Ca ion electrode, pH electrode, hyperchannel micro-injection pump, peristaltic pump and well heater to realize Ca ion concentration, pH value, flow velocity, flow, the isoparametric accurate control of temperature, the altitude simulation of realizing the Human Physiology environment reproduces, this device has fault alarm function and data recording function, makes things convenient for the user that device is controlled to realize the research to bio-medical material mineralising performance.
Description of drawings
Fig. 1 is a block diagram of the present utility model;
Fig. 2 is a structural representation of the present utility model;
Fig. 3 is a working routine block diagram of the present utility model.
Embodiment
Embodiment 1
As shown in Figure 1, analyser connects Ca ion electrode, pH electrode, temperature sensor (I) and temperature sensor (II) respectively, and be electrically connected mutually with PC, PC also is connected with peristaltic pump (I), peristaltic pump (II), hyperchannel micro-injection pump, well heater, stirrer (I) and stirrer (II) respectively.By the PC program be provided with can pick-up unit in the signal of Ca ion electrode, pH electrode and temperature sensor (I) and temperature sensor (II), can realize control, by the adjustment of the pH value of Ca liquid concentration, P liquid concentration and simulated solution in the control multichannel injection pump implement device to peristaltic pump flow velocity and flow.PC is by control heater implement device temperature control, and adopts the temperature equalization of stirrer (I) accelerator, by control stirrer (II) realize simulated body fluid ion concentration in the conditioning chamber evenly.
As shown in Figure 2, ageing chamber 1, sample placement unit 3, conditioning chamber 10,1000W well heater 4 and JB-2 type stirrer (I) 18 place in the water bath 9 in the intelligent type multi-parameter external mineralizing analogue means described in the utility model, ageing chamber 1, sample placement unit 3, conditioning chamber 10 are counterclockwise distribution, and be connected by the silica gel pipeline 7 of Φ 4mm * 6mm, ageing chamber 1 and sample placement unit 3 drive by BQ-50-1J type peristaltic pump (I) 2, and conditioning chamber 10 and ageing chamber 1 drive by BQ-50-1J type peristaltic pump (II) 17.Water bath 9 adopts well heaters 4 to heat, and makes temperature equalizations in the water bath 9 by JB-2 type stirrer (I) 18.WZ-50S type micro-injection pump 8 is connected with conditioning chamber 10 with the additional pond 6 of ion by the silica gel pipeline 7 of Φ 4mm * 6mm.ELIT9804 type analysis instrument 11 is connected with E-201-C type pH electrode 12, ELIT8041 type Ca ion electrode 13, ELIT8701 type temperature sensor (I) 14 and ELIT8701 type temperature sensor (II) 15, temperature sensor (I) 14 places in the conditioning chamber 10, and temperature sensor (II) places in the water bath 9.Peristaltic pump in the described device (I) 2, peristaltic pump (II) 17, well heater 4, hyperchannel micro-injection pump 8, analyser 11, stirrer (I) 18 and stirrer (II) 16 and PC 5 are by being electrically connected.
The simulated solution pipeline requires:
(1) bear the hydraulic pressure of 20kPa in the simulated solution connecting pipe deferent, continue 24h, should be non-leakage or break;
(2) the simulated solution connecting tube is at BQ-50-1J type peristaltic pump (I) and (II) continuous firing 48h under the maximum working load, should be non-leakage or break.
(3) the simulated solution pipeline replenishes to combine between the pond with ageing chamber, sample placement unit, conditioning chamber, micro-injection pump and ion and requires:
Simulated solution pipeline and ageing chamber, sample placement unit, conditioning chamber, micro-injection pump and ion replenish between the pond should be in conjunction with closely, under the hydraulic pressure of 100kPa, and should be non-leakage.
As shown in Figure 3, the work of the external mineralizing analogue means of intelligent multi-parameter shown in the utility model is finished by coordinations such as signal detection system, control system and man-machine conversation software control.The work of finishing mainly contains the following aspects:
(1) collection and the processing of signals such as temperature, Ca ion concentration, pH value, simulated solution flow velocity, simulated solution flow;
(2) Control Software of the peristaltic pump of follow procedure design, temperature, hyperchannel micro-injection pump;
(3) critical data in the access use;
The temperature sensor accuracy requirement that the utility model adopted is within ± 0.5 ℃, the accuracy requirement of pH electrode is within ± 0.1, the accuracy requirement of Ca ion electrode is within ± 0.05mM, and the peristaltic pump accuracy requirement is at ± 10ml/h, and the micro-injection pump accuracy requirement is at ± 1ml/h.
The utility model device mainly is divided into following step when using:
(1) artificial emptying pipeline;
(2) import service data last time;
(3) change or replenish fresh simulated solution;
(4) heating simulated solution;
(5) calibrate each electrode;
(6) put into sample and carry out the mineralising processing;
(7) record data and effluent discharge.
As mentioned above, can realize the utility model preferably.
Claims (4)
1, a kind of intelligent type multi-parameter external mineralizing analogue means is characterized in that comprising
---concentration adjustment chamber, ageing chamber, sample placement unit; Described concentration adjustment chamber links to each other with the ageing chamber by pipeline, and a peristaltic pump is set in the middle of the described pipeline; Described ageing chamber links to each other with the sample placement unit by pipeline, and a peristaltic pump also is set in the middle of the described pipeline; Described sample placement unit links to each other with the concentration adjustment chamber by pipeline; Under the effect of peristaltic pump, simulated body fluid circulates in concentration adjustment chamber, ageing chamber, sample placement unit;
Described concentration adjustment chamber, ageing chamber, sample placement unit all are arranged in the water bath, and a well heater also is set in the described water bath;
Described concentration adjustment chamber replenishes the pond by pipeline with hyperchannel micro-injection pump and several ions and links to each other;
---a signal detection system, described signal detection system comprises that an analyser, one are arranged at the Ca ion electrode of concentration adjustment chamber, a pH electrode that is arranged at the concentration adjustment chamber, a Temperature Detector that is arranged in the water bath, and described Ca ion electrode, pH electrode and Temperature Detector all are electrically connected with analyser;
Described peristaltic pump, well heater, hyperchannel micro-injection pump and analyser all are electrically connected with PC, Control Software is housed in the PC, by the action of PC control peristaltic pump, well heater, hyperchannel micro-injection pump and analyser, realize the simulation reconstruction of Human Physiology environment.
2, device according to claim 1 is characterized in that also comprising a stirrer that is arranged in the water bath, and described stirrer is electrically connected with the PC of machine control stirrer.
3, device according to claim 2 is characterized in that also comprising a stirrer that is arranged at the concentration adjustment chamber, and described stirrer is electrically connected with the PC of control stirrer.
4, device according to claim 3 is characterized in that described signal detection system also comprises a Temperature Detector that is arranged at the concentration adjustment chamber and is electrically connected with analyser.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720047892 CN200996959Y (en) | 2007-01-26 | 2007-01-26 | External analog mineralizer of intelligent multi-parameter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720047892 CN200996959Y (en) | 2007-01-26 | 2007-01-26 | External analog mineralizer of intelligent multi-parameter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN200996959Y true CN200996959Y (en) | 2007-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200720047892 Expired - Lifetime CN200996959Y (en) | 2007-01-26 | 2007-01-26 | External analog mineralizer of intelligent multi-parameter |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101013123B (en) * | 2007-01-26 | 2010-08-11 | 华南理工大学 | Intelligent type multi-parameter external mineralizing analogue means |
| CN104111319A (en) * | 2014-07-29 | 2014-10-22 | 东北农业大学 | Air-guiding cylindrical vertical type device for measuring soil mineralization rate |
-
2007
- 2007-01-26 CN CN 200720047892 patent/CN200996959Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101013123B (en) * | 2007-01-26 | 2010-08-11 | 华南理工大学 | Intelligent type multi-parameter external mineralizing analogue means |
| CN104111319A (en) * | 2014-07-29 | 2014-10-22 | 东北农业大学 | Air-guiding cylindrical vertical type device for measuring soil mineralization rate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20071226 Effective date of abandoning: 20070126 |