CN200982965Y - A point sampling probe - Google Patents

Abstract

The utility model provides a sample needle comprising a sample needle glass tube used for containing the sample solution, a push bar which can reciprocate on the inner wall of the sample needle glass tube, an insulation spout and a metallic needle tube whose two ends are respectively connected with the sample needle glass tube and the insulation spout. Optimally, among all the sample needles above-mentioned, the delivery end of the insulation spout is in a cone shape and the inner diameter of the insulation spout is 0.1 millimeter. Compared with the prior art of the sample needle and according to the utility model, the front end of the metallic needle tube coating a section of the delivery end extends to be a quartz tube to solve the infiltrative problem. Through the extension of a port of the quartz tube, the diameter of the spout is reduced, the volatilization of the dissolvent is improved and the width of the strip of the sample is reduced.

Description

A kind of spotting needle

Technical field

The utility model relates to chemical analysis technology, particularly a kind of spotting needle, but on the point sample instrument of its application of thin layer chromatography, also can be applicable to the point sample of biochip.

Background technology

When point sample, during especially with the supporting use of point sample instrument, generally adopt the spotting needle point sample.Generally speaking, wish that the spot of point sample is smaller, and this just needs solvent evaporates fast, thereby make that the lip-deep spot diffusion of point sample is less.

Existing spotting needle by the syringe of centre, be positioned at the push rod of syringe upper end and be positioned at syringe lower end metal needle three parts and constitute, wherein, hold solution in the syringe and hold tightly together, when promoting push rod with metal needle, solution is released by the metal needle point, to finish point sample.Because the infiltration feature of metal is very remarkable,, form the liquid pearl so outer wall becomes easily infected by point sample liquid when point sample.On the spotting needle outer wall, soak into liquid, can adopt the spotting needle of the structure of bag Teflon, but this technology is very complicated, and cost is very high.

Summary of the invention

The purpose of this utility model provides a kind of spotting needle, and it can solve the problem that the spotting needle outer wall soaks into.

Above-mentioned purpose of the present utility model realizes by following technical proposal:

A kind of spotting needle comprises:

The spotting needle glass tube is used to hold described spotting solution;

Can be along the reciprocating push rod of described spotting needle glass tube inwall;

Insulation nozzle; And

The metal needle tubing that two ends are connected with described spotting needle glass tube and described insulation nozzle respectively.

Preferably, in above-mentioned spotting needle, the endpiece of described insulation nozzle is cone shape.

Preferably, in above-mentioned spotting needle, the internal diameter of described insulation nozzle is 0.1 millimeter.

Compare with the spotting needle of prior art, according to utility model, the front end of metal needle tubing coats the one section outlet end and is stretched as quartz ampoule and solves the infiltration problem, again by to the stretching of quartz ampoule port to reduce the diameter of nozzle, improve the volatilization of solvent, reduce the width of the band of point sample.

The accompanying drawing summary

By below in conjunction with the description of accompanying drawing to the utility model preferred embodiment, can further understand the purpose of this utility model, feature and advantage, wherein:

Fig. 1 is the making synoptic diagram according to spotting needle of the present utility model.

Fig. 2 is the structural representation according to spotting needle of the present utility model.

Fig. 3 shows the squiggle of the high direct voltage that applies at metal needle tubing 13.

Embodiment

Fig. 1 shows the structure according to the spotting needle of an embodiment of the utility model.As shown in Figure 1, spotting needle 1 comprises push rod 11, spotting needle glass tube 12, metal needle tubing 13 and insulation nozzle 14.Push rod 11 is positioned at a wherein end port of spotting needle glass tube 12, can be reciprocating along the inwall of spotting needle glass tube 12, thus the spotting solution 4 in the spotting needle glass tube 12 is pushed insulation nozzle 14.The two ends of metal needle tubing 13 are nested into spotting needle glass tube 12 other end ports and insulation nozzle 14 respectively, and sidewall closes through lead and high-voltage power module (not shown) lotus root.

Referring to Fig. 1, carry point sample sample 3 on the objective table 2, for example the thin-layer silicon offset plate.When on metal needle tubing 13, applying a high direct voltage, to between metal needle tubing 13 and objective table 2, form high-voltage electric field, therefore push the solution generation ionization in the insulation nozzle 14, thereby the exit at nozzle forms spraying, along with the motion of spotting arm, this spraying arrives the point of sample that the point sample sample surfaces of placing on the objective table 2 promptly forms different shape.

Fig. 2 shows the principle of electron spray.As shown in Figure 2, the high-voltage electric field between metal needle tubing 13 and the objective table 2 makes solution generation ionization, and negative ion wherein attracted to the other end of metal needle tubing 13, then assembles a large amount of positive charge ions at the semi-moon shaped liquid surface of needle point.Repel mutually between positive charge ion, and expand away from the liquid surface of needle point, along with diminishing of droplet, electric field intensity strengthens gradually, and superfluous positive charge finally forms droplet, sputters out from the endpiece of nozzle 14, forms and sprays.Preferably, this endpiece is cone shape.

Fig. 3 shows the squiggle of the high direct voltage that applies at metal needle tubing 13.By the adjustment of waveform, can guarantee the separation sputter of various drops, make drop slow down again, thereby prolong the volatilization time of solution at the sputtering rate in low level district.The prolongation of solution evaporation time can make more solution evaporation, and this has alleviated the diffusion of point sample band, thereby makes band thinner.Preferably, the repetition frequency of high direct voltage waveform can be set at 500Hz.

In according to spotting needle of the present utility model, because the quartz wool thin glass tube all do not soak into for general organic solvent, and nozzle is cone-shaped, and the solvent that has therefore improved outer wall greatly soaks into problem.In addition, realized electron spray mode point sample by between metal needle tubing and objective table, applying high-voltage electric field.In this manner, nozzle diameter is more little, and then the volatilization effect of solvent is good more under identical electrostatic field, and preferably, the internal diameter of insulation nozzle of the present utility model is not more than 0.1 millimeter, thereby can reach very high solvent evaporates effect.

Under the effect of the high-voltage electrostatic field of electron spray, at positive ion electrospray under pattern, oxidation reaction can take place in the tip of spray needle, thereby the needle point to metal has corrosive effect (even the needle point skin has coated the also still generation of inevitable this oxidizing process of Teflon), but in spotting needle of the present utility model, because front end is a quartz nozzle, thereby thoroughly solved the problem of needle point corrosion.

More than by example the utility model has been made detailed description, but it should be understood that, foregoing description only has illustrative nature, should not be construed as qualification to the utility model scope and spirit, for the one of ordinary skilled in the art, on the basis of above-mentioned example, need not can propose various modifications or change through performing creative labour, therefore scope and spirit of the present utility model are limited by claim.

Claims (3)

1, a kind of spotting needle is characterized in that, comprising:

The spotting needle glass tube is used to hold described spotting solution;

Can be along the reciprocating push rod of described spotting needle glass tube inwall;

Insulation nozzle; And

The metal needle tubing that two ends are connected with described spotting needle glass tube and described insulation nozzle respectively.

2, spotting needle as claimed in claim 1, wherein, the endpiece of described insulation nozzle is cone shape.

3, spotting needle as claimed in claim 1, wherein, the internal diameter of described insulation nozzle is 0.1 millimeter.

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