CN200951212Y - Biological membrane for wound-surface protection - Google Patents

Abstract

The utility model discloses a biotype wound-protecting film, which comprises a substrate (1) which is made of animal intestinal membrane dealt with nonaldehyde fixatives crosslinked fixation and removed antigen and an active modification layer (2) which contains a fibronectin which can be attached to cells, a laminin or vitronectin on the surface of the substrate (1). The utility model has the advantages of lightweight, softness and convenient operation since the film is made of the thin and tenacious animal intestinal membrane. By adding the effect of the active modification of surface, the wound-protecting film can remove effectively immunogenicity because the intestinal membrane belongs to half permeable membrane with little and dense micropores, is breathable and permeable, but can not permeate bacteria and is dealt with removing antigen. Furthermore, the wound-protecting film can collect epitheliums and fibroblasts to promote wound healing, or can release anti-bacterial drugs and enhance anti-infection effect, the effects of protecting wound are better than that of the wound dressings or protecting films made from pigskin.

Description

Biological wound-protecting film

Technical field

This utility model relates to a kind of medical supplies that are used for trauma care protection wound surface, belongs to two class medical apparatus and instruments.

Background technology

Because human body self has perfect repair mechanism to wound, many medium and small wounds only need be good with protecting wound surface, makes it not infected, leans on the repair mechanism of self just can heal voluntarily.The primary measure of general traumatism treatment is the wound surface that cleans, sterilizes, and with protecting wound dressing with protecting wound surface, sends out after preventing and infects.So the development of protecting wound dressing in recent years is very fast, the time have various wound-protecting films to release.But be synthetic material membrane mostly, as silicone rubber membrane, polyurethane film, nylon membrane, dacron membrane, polyethylene film, polypropylene film etc., after using after a while, people find gradually, the histocompatibility of synthetic material is bad, therapeutic effect is unsatisfactory, someone attempts to make wound-protecting film with natural biologic material such as collagen, but collagen poor mechanical property, also will strengthen with synthetic film, though this collagen synthetic material composite membrane can improve its histocompatibility, thickness increases the flexibility variation, breathability is not good enough, and serviceability is bad.Human chitosan and chitosan collagen complex system wound-protecting film are also arranged, but mechanical strength is poor, toughness and flexibility are not good enough, fail to apply, have the human Corii Sus domestica to make wound-protecting film in recent years, but just with the fixing chrome tanning system treatment technology that reaches of traditional glutaraldehyde, not removing antigen specially handles, the residual toxicity that aldehyde is arranged goes antigen thoroughly not have weak rejection, and the dry state flexibility is poor, and doing the back pore increases, every bacterium property variation, serviceability and therapeutic effect are all undesirable, influence it and apply.

Summary of the invention

The purpose of this utility model provides a kind of good biocompatibility, soft tough and tensile, ventilative saturating bacterium, biological wound-protecting film easy to use.

Technical solution of the present utility model is: the biotype wound-protecting film, but it is by the activity modifying layer of film formed base material of animal intestine and lip-deep fibronectin, laminin or the vitronectin that contains adherent cell that be located at described base material or antimicrobial sustained-release layer and form.

Animal tissue easily is degraded by microorganisms or decomposes, need to make it crosslinked fixing with fixative, use the glutaraldehyde agent that fixes traditionally, residual toxicity is arranged, we select the no-aldehyde fixative for use, as epoxide, two acid diamides, vulcabond, Polyethylene Glycol or carbodiimides, just there is not this shortcoming, with the epoxide is example, when the application epoxide replaces aldehydes to fix reagent, because epoxide is very unstable, the open loop cross-linking reaction takes place easily, the control reaction condition can accomplish to make the cross-linking products of its collagen protein very stable, degraded is not easily only grown at regenerating tissues, when propagation needs its silkworm erosion, secrete kallikrein, fibrinolysin, under the collaborative collagenase effect of glucocorticoid, it slowly could be decomposed into polypeptide and aminoacid, and absorb.A kind of like this passive type degraded is synchronous with the regeneration of tissue, is that the reproducibility that helps organizing is most repaired, and does not have the residual toxicity of aldehydes; According to modern immunology theory, the antigenicity of animal tissue mainly is to look like to cause that by the active group of some specific position in the protein and special structure these active groups mainly are-OH-NH ₂-SH etc., special structure picture then mainly is because some special hydrogen bond of collagen molecules coiled strand causes, when handling animal tissue, easily combine with these groups with one or more with the active reagent (as anhydride, acyl chlorides, amide, epoxide, halomethane etc.) that these groups react, it is closed, thereby effectively remove its antigen, simultaneously, also use strong hydrogen bonding reagent (as guanidine compound), displacement causes the hydrogen bond of special structure picture, changes its structure picture, just can effectively eliminate its antigenicity.Be provided with the activity modifying layer at substrate surface, but the fibronectin or laminin or the vitronectin that wherein contain adherent cell, to improve wound-protecting film to epithelial cell and fibroblastic adhesiveness, enable to collect epithelial cell and fibroblast, promote wound repair, healing; Perhaps contain the release-controlled coated of broad spectrum antibiotic, make wound-protecting film have antibiotic, anti-infective effect at the activity modifying layer.

Described no-aldehyde fixative is easily and the reagent of protein molecule generation cross-linking reaction such as in epoxide, two acid diamides, vulcabond, Polyethylene Glycol or the carbodiimides reagent one or both, and the epoxide here can be a monoepoxide It also can be di-epoxide

Here R=H, C _nH _2n+1-, n=0-10; Can also be low polyepoxide, as poly(propylene oxide).

Described active reagent can be small molecular organic acid acid anhydride, acyl chlorides, amide, epoxide or halomethane; Strong hydrogen bonding reagent is guanidine compound.

The utility model has the advantages that: it is to make with very thin and tough and tensile animal goldbeater's skin, light weight and softness is easy to use.Goldbeater's skin belongs to semipermeable membrane; micropore is small and dense, and is ventilative and steam permeability is good, but do not see through antibacterial; having carried out the multi-faceted antigen that removes again handles; effectively remove immunogenicity, add surface activity and modify, can collect epithelial cell and fibroblast; promote wound healing; or can slow release antimicrobial drug, increase anti-infectious function, its serviceability and to protect dressing or protecting film that the wound effect makes than Corii Sus domestica good.

Description of drawings

Accompanying drawing 1 is the cutaway view of this utility model embodiment;

1, base material, 2, activity modifying layer or antimicrobial sustained-release layer.

The specific embodiment

Embodiment: as shown in Figure 1, the biotype wound-protecting film, by being that base material 1 constitutes, but on the surface of base material 1, be provided with activity modifying layer 2 or antimicrobial sustained-release layer 2 that the fibronectin of adherent cell, laminin or vitronectin are contained in inside through the crosslinked animal goldbeater's skin fixing and that go antigen to handle of no-aldehyde fixative.

The preparation method of biotype wound-protecting film of the present utility model may further comprise the steps:

(1), selects materials: collect the fresh animal small intestinal;

(2), pretreatment: clean, sterilization is struck off mucous membrane of small intestine and lower floor's connective tissue with specific purpose tool, gets tough and tensile thin film and prunes the smooth dry film material that gets;

(3), defat: with fat and the oil-soluble impurities in the organic solvent extracting film material;

(4), crosslinked fixing: as to use the collagen molecules in the epoxy crosslinked fixing substrate 1 under the room temperature;

(5), remove antigen: use in the active reagent butyryl oxide. closing membrane material collagen protein specific activity group-OH or-NH ₂Or-SH, and, change special structure picture with the special hydrogen bond in the Tris solution displacement film material collagen molecules coiled strand of guanidine hydrochloride, promptly get base material 1.

(6), surface activity is modified: with absorption, adhesion method fibronectin, laminin or vitronectin are adhered to base material 1 surface, form activity modifying layer 2, or with the biological slime colloid with coat base material 1 surface after the spectrum antimicrobial drug mixes, form antimicrobial sustained-release layer 2.

(7), post processing: vacuum drying, typing is packed, and with epoxide or radiation sterilization, obtains finished product.

Claims (1)

1, a kind of biological wound-protecting film, it is characterized in that: it is by the film formed base material of animal intestine (1) and be located at described base material (1) but the lip-deep activity modifying layer (2) that contains fibronectin, laminin or the vitronectin of adherent cell, and perhaps antimicrobial sustained-release layer (2) is formed.

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