CN105879104A - Biological wound protecting film and preparation method thereof - Google Patents
Biological wound protecting film and preparation method thereof Download PDFInfo
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- CN105879104A CN105879104A CN201410649580.7A CN201410649580A CN105879104A CN 105879104 A CN105879104 A CN 105879104A CN 201410649580 A CN201410649580 A CN 201410649580A CN 105879104 A CN105879104 A CN 105879104A
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Abstract
The invention discloses a biological wound protecting film and a preparation method thereof. The biological wound protecting film is formed by using animal goldbeater's skin subjected to cross-linking fixation by non-aldehyde fixing agents and antigen extraction treatment as a substrate. An active modification layer containing cell-attachable fibronectin, laminin or vitronectin or an antibacterial sustained release layer containing antibacterial medicine is arranged on the surface of the substrate. The biological wound protecting film is prepared from the thin and tough animal goldbeater's skin, so that the weight is light; softness is realized; and the use is convenient. The goldbeater's skin belongs to a semipermeable membrane, has small and dense micropores, good air permeability, good steam permeability and germ impermeability; through all-round antigen extraction treatment, the immunogenicity is effectively removed; through surface active modification, epithelial cells and fibroblasts can be enrolled to promote wound healing; or the antibacterial medicine can be slowly released, and the anti-infection effect is enhanced; and the use performance and the wound protection effects are better than those of dressing or a protection film made of pigskin.
Description
Technical field
The present invention relates to a kind of medical supplies protecting wound surface in trauma care, belong to two class medical apparatus and instruments.
Background technology
Owing to human body self has perfect repair mechanism, many medium and small wounds, it is only necessary to by protecting wound surface to wound
Good so that it is the most infected, the repair mechanism by self just can self-heal.Primarily arranging of general traumatism treatment
Executing is cleaning, sterilization wound surface, is got up by protecting wound surface by protective dressing, sends out and infect after preventing.So, in recent years
Carry out the development of protective dressing quickly, time have various wound-protecting film to release.But it is synthetic material membrane mostly, as
Silicone rubber membrane, polyurethane film, nylon membrane, dacron membrane, polyethylene film, polypropylene film etc., through after a while
After application, people gradually find, the histocompatibility of synthetic material is bad, and therapeutic effect is unsatisfactory, has people
Attempt to manufacture wound-protecting film with natural biologic material such as collagen, but collagen poor mechanical property, also with synthesis film
Strengthening, though this collage synthesis Material cladding film can improve its histocompatibility, but thickness increases flexibility and becomes
Difference, breathability is not good enough, and serviceability is bad.Also there are people's chitosan and chitosan collagen-based composite wound-protecting film,
But mechanical strength is poor, toughness and flexibility be not good enough, fails popularization and application, has people to make wound-protecting film with Corii Sus domestica in recent years,
But simply fix and chrome tanning treatment technology with traditional glutaraldehyde, do not exclusively carry out except antigen processes, have aldehyde
Residual toxicity, go antigen the most thoroughly to there is weak rejection, dry state flexibility is poor, and dry after pore increase,
Being deteriorated every bacterium property, serviceability and therapeutic effect are the most undesirable, affect its popularization and application.
Summary of the invention
It is an object of the invention to provide a kind of good biocompatibility, soft impermeable bacterium tough and tensile, ventilative, easy to use
Biological wound-protecting film and preparation method thereof.
The technical solution of the present invention is: biotype wound-protecting film, it by fixing through the crosslinking of no-aldehyde fixative and
The animal goldbeater's skin going antigen to process is that base material is constituted.
Animal tissue is easily degraded by microorganisms or decomposes, and need to be allowed to crosslinking with fixative fixing, and conventionally used penta
Dialdehyde makees fixative, has residual toxicity, and we select no-aldehyde fixative, as epoxide, two acid diamides,
Diisocyanate, Polyethylene Glycol or carbodiimides, just do not have this shortcoming.As a example by epoxide, when answering
When replacing aldehydes to make fixating reagent with epoxide, owing to epoxide is the most unstable, easily there is open loop crosslinking
Reaction, controls reaction condition and can accomplish that the cross-linking products making its collagen protein is the most stable, degrade the most easily, only
Have when its silkworm need to be lost by regenerating tissues growing multiplication, secret out of kallikrein, fibrinolysin, glucocorticoid
Under collaborative collagenase effect, polypeptide and aminoacid could be decomposed slowly to, and absorbed.Such a
Passive type degraded is Tong Bu with the regeneration of tissue, is that the reproducibility most beneficial for tissue is repaired, and without aldehyde
The residual toxicity of class；Theoretical according to Immunology Today, the antigenicity of animal tissue mainly by protein some
The active group of specific position and particular conformation cause ground, these active groups mainly-OH ,-NH2,-SH
Deng, particular conformation, then some special hydrogen bond mainly due to collagen molecules coiled strand causes, and is processing
During animal tissue, with one or more activating agent easily reacted with these groups (such as anhydride, acyl chlorides, acyl
Amine, epoxide, halomethane etc.) be combined with these groups, it is closed, thus is effectively removed it
Antigen, meanwhile, also application strong hydrogen bonding reagent (such as guanidine compound), displacement causes the hydrogen bond of particular conformation, changes
Become its conformation, just can effectively eliminate its antigenicity.
As a kind of prioritization scheme, it is provided with activity modifying layer on substrate surface, wherein or thin containing adhering to
The fibronectin of born of the same parents or laminin，LN or vitronectin, to improve wound-protecting film to epithelial cell and fibroblast
Adhesiveness, enable collection epithelial cell and fibroblast, promote wound repair, healing；Or in activity
Decorative layer is contained within the release-controlled coated of broad spectrum antibiotic, makes wound-protecting film have antibacterial, anti-infective effect.
The preparation method of the biotype wound-protecting film of the present invention comprises the following steps:
(1), select materials: collect fresh animal small intestinal；
(2), pretreatment: clean, sterilization, remove mucous membrane of small intestine and lower floor's connective tissue, take tough and tensile thin film also
Prune smooth be dried to obtain base material；
(3), defat: with the fat in organic solvent extracting base material and oil-soluble impurities；
(4), crosslinking is fixing: use the collagen molecules in no-aldehyde fixative crosslinking fixing substrate；
(5), antigen is removed: use the specific activity group-OH or-NH in activating agent closed substrate protein2
Or-SH, and with the special hydrogen bond in strong hydrogen bonding reagent displacement base material protein molecule coiled strand, change special structure
As.
As a kind of prioritization scheme, it is additionally provided with the step being provided with activity modifying layer on substrate surface, and with inhaling
Fibronectin, laminin，LN or vitronectin are adhered to substrate surface by attached, adhesion method, form activity modifying
Layer, or coat substrate surface with biological slime colloid after mixing with spectrum antibacterial medicine, formation antimicrobial sustained-release layer.
No-aldehyde fixative described in the preparation method of biotype wound-protecting film is for easily to hand over protein molecule
The reagent of connection reaction, as epoxide, two acid diamides, diisocyanate, Polyethylene Glycol or carbodiimides try
One or both in agent, epoxide here can be monoepoxide can also be di-epoxide, this
In R=H, CnH2n+1-, n=0-10；Can also is that low polyepoxide, such as poly(propylene oxide).
Activating agent described in the preparation method of biotype wound-protecting film can be small molecular organic acid acid anhydride, acyl chlorides,
Amide or epoxide；Strong hydrogen bonding reagent is guanidine compound.
The invention have the advantage that it is made with the thinnest and tough and tensile animal goldbeater's skin, light weight and soft, use
Convenient.Goldbeater's skin belongs to semipermeable membrane, and micropore is small and dense, breathes freely and steam permeability is good, but be not through antibacterial, again through many
Orientation except antigen process, effectively remove immunogenicity, add surface activity modify, can collect epithelial cell and
Fibroblast, wound healing；Or can sustained-release antibacterial medicine, increase anti-infectious function, its serviceability and
Protect the wound dressing made than Corii Sus domestica of effect or protecting film is good.
Accompanying drawing explanation
Accompanying drawing 1 is the sectional view of the embodiment of the present invention；
Detailed description of the invention
As it is shown in figure 1, biotype wound-protecting film, fixed by through the crosslinking of no-aldehyde fixative and go antigen to process
Animal goldbeater's skin is that base material 1 is constituted, be provided with on the surface of the substrate 1 containing can adherent cell fibronectin,
The activity modifying layer 2 of laminin，LN or vitronectin or the antimicrobial sustained-release layer 2 containing antimicrobial drug.
The preparation method of the biotype wound-protecting film of the present invention comprises the following steps:
(1), select materials: collect fresh animal small intestinal；
(2), pretreatment: clean, sterilization, strike off mucous membrane of small intestine and lower floor's connective tissue with specific purpose tool, take
Tough and tensile thin film and prune smooth be dried to obtain film material；
(3), defat: with the fat in organic solvent extracting film material and oil-soluble impurities；
(4), crosslinking is fixing: use the collagen molecules in epoxy crosslinked fixing film material under room temperature；
(5), antigen is removed: use the specific activity group in activating agent butyryl oxide. closing membrane material collagen protein
-OH or-NH2Or-SH, and with the spy in the Tris solution replacement film material collagen molecules coiled strand of guanidine hydrochloride
Different hydrogen bond, changes particular conformation, obtains base material 1.
(6), surface activity is modified: with absorption, adhesion method by fibronectin, laminin，LN or vitronectin
Adhere to base material 1 surface, form activity modifying layer 2, or painting after mixing with spectrum antibacterial medicine with biological slime colloid
It is distributed in base material 1 surface, forms antimicrobial sustained-release layer 2.
(7), post processing: be vacuum dried, sizing, packaging, with epoxide or radiation sterilization, obtain finished product.
Claims (6)
1. the preparation method of a biotype wound-protecting film, it is characterised in that: it comprises the following steps:
A), select materials: collect fresh animal small intestinal；
B), pretreatment: clean, sterilization, remove mucous membrane of small intestine and lower floor's connective tissue, take tough and tensile thin film and repair
Cut flat whole be dried to obtain base material (1)；
C), defat: with the fat in organic solvent extracting film material and oil-soluble impurities；
D), crosslinking is fixing: use the collagen molecules in the fixing film material of no-aldehyde fixative crosslinking；
E), remove antigen: use activating agent closing membrane material collagen protein in specific activity group-OH or
-NH2Or-SH, and with the special hydrogen bond in the collagen molecules coiled strand of strong hydrogen bonding reagent displacement film material, change
Become particular conformation, obtain base material (1).
Preparation method the most according to claim 1, it is characterised in that: it is additionally provided with on base material (1) surface
On carry out the step of activity modifying, i.e. with absorption, adhesion method by fibronectin, laminin，LN or vitronectin
Adhere to base material (1) surface, form activity modifying layer (2)；Or mix in extensive pedigree antibiotic with biological slime colloid
Coat base material (1) surface, form antimicrobial sustained-release layer (2).
Preparation method the most according to claim 1, it is characterised in that: described no-aldehyde fixative is
Easy and protein molecule crosslinks the reagent of reaction, such as epoxide, two acid diamides, diisocyanate, gathers
One or both in ethylene glycol or carbodiimide reagent, epoxide here can be monoepoxide,
Can also be di-epoxide R=C herenH2n+1-, n=0-10, it is also possible to be low polyepoxide, such as polycyclic
Ethylene Oxide.
Preparation method the most according to claim 1, it is characterised in that: described activating agent can be
Small molecular organic acid acid anhydride, acyl chlorides, amide, epoxide or halomethane；Strong hydrogen bonding reagent is guanidine compound.
5. the biotype wound-protecting film using preparation method described in claim 1 to obtain, it is characterised in that:
It is that base material (1) is constituted by cross-linking animal goldbeater's skin that is fixing and that go antigen to process through no-aldehyde fixative.
6. the biotype wound-protecting film using preparation method described in claim 2 to obtain, it is characterised in that:
The surface of described base material (1) sets containing the fibronectin of adherent cell, laminin，LN or glass connecting egg
White activity modifying layer (2), or the antimicrobial sustained-release layer (2) containing antimicrobial drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410649580.7A CN105879104A (en) | 2014-11-05 | 2014-11-05 | Biological wound protecting film and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410649580.7A CN105879104A (en) | 2014-11-05 | 2014-11-05 | Biological wound protecting film and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105879104A true CN105879104A (en) | 2016-08-24 |
Family
ID=56698333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410649580.7A Pending CN105879104A (en) | 2014-11-05 | 2014-11-05 | Biological wound protecting film and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105879104A (en) |
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2014
- 2014-11-05 CN CN201410649580.7A patent/CN105879104A/en active Pending
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160824 |