CN200944106Y - Clenobuterol hydrochloride colloidal gold detecting test paper - Google Patents
Clenobuterol hydrochloride colloidal gold detecting test paper Download PDFInfo
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- CN200944106Y CN200944106Y CN 200620019375 CN200620019375U CN200944106Y CN 200944106 Y CN200944106 Y CN 200944106Y CN 200620019375 CN200620019375 CN 200620019375 CN 200620019375 U CN200620019375 U CN 200620019375U CN 200944106 Y CN200944106 Y CN 200944106Y
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- clenbuterol
- test paper
- utility
- colloidal gold
- adsorptive pads
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Abstract
The utility model relates to a detecting test paper used in Clenbuterol Hydrochloride colloidal gold method, which belongs to the test paper of biological immunity method. Clenbuterol Hydrochloride is equal to Ractopamine, which is a drug of Beta exhilarant type that is forbidden in feed and livestock breeding production. The utility model fixates chloromycetin monoclonal antibody 2 chloromycetin-carrier antigen 3 and antimouse IgG antibody 4 which are marked by colloidal gold on a pyroxylin film 6 and on a base plate 7. The clenbuterol-carrier antigen 3 acts as a detecting band, and the antimouse IgG antibody 4 acts as a reference band. The utility model uses principles of chromatography type single-step competition method, compares colors of the upper and lower bands and detects semiquantitatively residual quantity of Clenbuterol in blood serum or urine sample in 5 to 10 minutes with the precision of 1ppb (1ng/m1). The utility model can quickly, accurately and semiquantitatively detect residual quantity of Clenbuterol and meet test requirements of food safety, feed enterprises and meat producing plants as well as governmental test departments.
Description
Affiliated technical field
The utility model belongs to the mensuration test paper and the preparation method of biology immunization method.
Background technology
Clenobuterol hydrochloride (Clenbuterol Hydrochloride), claim clenbuterol hydrochloride again, it is the beta-stimulants similar drug that feed and breeding production ban use of, this medicine is residual savings in livestock products, cause human produce acute poisoning and other influences by food chain, completely forbidden growth accelerator as edible animal.According to Ministry of Agriculture's " veterinary drug and other compound inventory of food animal forbidding " regulation, 10
-9Must not detect on the ppb order of magnitude.What use was more in detecting at present is euzymelinked immunosorbent assay (ELISA) (ELISA) kit, it has the Ag-Ab atopy, can qualitative sample result, false negative and the least possible false positive promptly do not appear, this method detects as the screening of a large amount of samples usually, need instruments such as microplate reader, hydro-extractor and analytical balance, analysis and determination step are all more loaded down with trivial details.For qualitative or quantitative test detection several different methods and technology are arranged, as use the high performance liquid chromatography HPLC or the vapor-phase chromatography GC of two or more detection principles, or band hyphen (hyphenated) detection technique, i.e. chromatogram and mass spectrometric hyphenated technique-GC-MS or LC-MS
n, obtain testing compound structure essence mass spectrogram thus.But this method needs large-scale instrument, needs special place and professional, the analysis cost height, and pre-treatment is more time-consuming.
Summary of the invention
The purpose of this utility model provide a kind of can half-quantitative detection Clenbuterol, colloidal gold method test paper and test paper fast and accurately.
The utility model is realized above-mentioned purpose by following approach:
Clenobuterol hydrochloride detects test paper, comprise the nitrocellulose filter 6 that is fixed on the base plate 7, it is characterized in that gold mark anti-clenbuterol monoclonal antibody 2, Clenbuterol coupled antigen 3, anti-mouse IgG antibody 4,, form composite bed along the vertical surperficial compartment of terrain of nitrocellulose filter (6) fixed order.
Described base plate 7 middle parts are nitrocellulose filter 6 fixedly, and nitrocellulose filter 6 one ends are connected with last adsorptive pads 5; And the other end or connect with following adsorptive pads 1, perhaps overlapping by gold mark anti-clenbuterol monoclonal antibody 2 parts that are fixed in nitrocellulose filter 6 one ends links to each other with following adsorptive pads 1 again.
Described adsorptive pads 1 down, gold mark anti-clenbuterol monoclonal antibody 2 reach upward, and there is self-adhesive paper on adsorptive pads 5 surfaces.
Above-mentioned film bar width is divided into three types of 2.5mm, 3.0mm, 4.0mm.
Wherein, serve as to detect band with Clenbuterol coupled antigen 3, serve as with reference to band with anti-mouse IgG antibody 4.
It is the capillary siphoning effect of utilizing adsorptive pads to form that the utility model detects principle, make detected antigen at first with the combining of the anti-clenbuterol monoclonal antibody generation competition form of colloid gold label, when the anti-clenbuterol monoclonal antibody of colloid gold label is excessive, remaining monoclonal antibody floats to and detects band, combines with the Clenbuterol coupled antigen and colour developing; And the anti-clenbuterol monoclonal antibody of the colloid gold label that combines with antigen, its V district detected antigen of binding site occupies, can only cross over the detection band and float to, detect band together and carry out colorimetric and obtain testing result with reference to being with C position point and anti-mouse IgG antibody non-specific binding.
The utility model is used one step of chromatography type competition law principle, come residual of kelengtelu amount in half-quantitative detection serum or the urine sample with colorimetric up and down in the test paper, with the precision of 1ppb (1ng/ml), in 5-10min, detect sample rapidly and accurately and whether contain clenobuterol hydrochloride, to determine whether clenobuterol hydrochloride exceeds standard, can satisfy food security the residual of kelengtelu amount is detected demand, be applicable to feed, meat producing plant and testing agency of government.
Compared with prior art, the utility model effect is self-evident, promptly has characteristics easy to use, that economy is quick, making is easy, with low cost.
Description of drawings
Fig. 1 is the utility model vertical view.
Fig. 2 is that adsorptive pads is marked the side view that the anti-clenbuterol monoclonal antibody partly overlaps with gold under the utility model.
Fig. 3 is the side view of adsorptive pads and cellulose nitrate film cascade under the utility model.
Embodiment
Be described further below in conjunction with accompanying drawing.
1, clenobuterol hydrochloride detects test paper
Among the figure, comprise that adsorptive pads 1, gold are marked anti-clenbuterol monoclonal antibody 2, Clenbuterol coupled antigen 3, anti-mouse IgG antibody 4, gone up adsorptive pads 5, nitrocellulose filter 6, base plate 7 down.Wherein, space 4mm fixed order between gold mark anti-clenbuterol monoclonal antibody 2, Clenbuterol coupled antigen 3, the anti-mouse IgG antibody 4.The anti-clenbuterol monoclonal antibody tires 15000.
Fig. 2 is pasted on base plate 7 by gold mark anti-clenbuterol monoclonal antibody 2 one ends with following adsorptive pads 1, and marks anti-clenbuterol monoclonal antibody 2 parts overlapping with the gold on nitrocellulose filter 6 surfaces; Last adsorptive pads 5 is pasted on base plate 7 and is with 4 one ends by reference, and overlaps with nitrocellulose filter 6 one ends.
Fig. 3 is pasted on base plate 7 by gold mark anti-clenbuterol monoclonal antibody 2 one ends with following adsorptive pads 1, and overlaps with nitrocellulose filter 6 parts; Last adsorptive pads 5 is pasted the same.
Following adsorptive pads 1, gold mark anti-clenbuterol monoclonal antibody 2 and on adsorptive pads 5 surfaces self-adhesive paper is arranged.
Described test paper, is with as reference with anti-mouse IgG antibody 4 as detecting band with Clenbuterol coupled antigen 3.
The film bar is existing smooth outward, width 2.5mm, and the material adhesion-tight, the liquid speed of dividing a word with a hyphen at the end of a line is not less than 5mm/min.
2, test paper using method
(1) specimen preparation
Serum: extract animal blood to be checked, centrifugal or leave standstill after get transparent supernatant and use; If serum has excessive haemolysis, serum is experimentized with behind one times of the distilled water diluting again, otherwise the analoids redness is too dark, influences test result.
Urine: directly test with urine, as muddiness, the dilution in 1: 1 of first centrifuging and taking supernatant or distilled water.
Feed: get the feed adding 10ml dilution to be measured that 1g one grinds, therebetween every firmly concussion of 2min; During 10min, leave standstill 1min after shaking all and get supernatant 100 μ l, analyze with test paper after doing 10 times of dilutions with dilution.
(2) operation
The test paper lower end is immersed in the sample to be tested, is no more than down adsorptive pads.Immerse the 30sec taking-up and set level, 10min reads the result.
(3) testing result
Negative:
Two bands all develop the color, and detect and be with color to be deeper than with reference to band color, not hydrochloric Clenbuterol in the interpret sample.
Two bands all develop the color, and detect the band color with identical or approaching with reference to the band color, and the clenobuterol hydrochloride content of interpret sample is lower than 1ppb (1ng/ml).
Positive:
Two bands all develop the color, and are shallower than with reference to the band color but detect the band color, illustrate that clenobuterol hydrochloride content surpasses 1ppb (1ng/ml) in the test sample.
Detect band and do not develop the color, with reference to the band colour developing, the clenobuterol hydrochloride content in the interpret sample is higher than 5ppb (5ng/ml).
Above result is as shown in the table:
| The detection band＞ | Detect band ≈ | The detection band＜ | Detection is with 0 | |
| With reference to the band colour developing | Do not contain | 1＜ | ＞1 | ＞5 |
Last table is the unit of detection limit with ppb or ng/ml all.Detect band＞, ≈,＜, the 0th, and relatively with reference to the band color.
Claims (3)
1, the clenobuterol hydrochloride colloidal gold method detects test paper, comprise the nitrocellulose filter (6) that is fixed in base plate (7), it is characterized in that gold mark anti-clenbuterol monoclonal antibody (2), clenobuterol hydrochloride coupled antigen (3), anti-mouse IgG antibody (4), along the vertical surperficial compartment of terrain of nitrocellulose filter (6) fixed order, form composite bed.
2, clenobuterol hydrochloride colloidal gold method according to claim 1 detects test paper, it is characterized in that fixedly nitrocellulose filter (6) of base plate (7) middle part, and nitrocellulose filter (6) one ends are connected with last adsorptive pads (5); And the other end or connect with following adsorptive pads (1) perhaps with by the gold mark anti-clenbuterol monoclonal antibody (2) that is fixed in nitrocellulose filter (6) one ends links to each other with following adsorptive pads (1).
3, clenobuterol hydrochloride colloidal gold method according to claim 1 and 2 detects test paper, and adsorptive pads (1), gold are marked anti-clenbuterol monoclonal antibody (2) and gone up adsorptive pads (5) surface under it is characterized in that self-adhesive paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620019375 CN200944106Y (en) | 2006-03-23 | 2006-03-23 | Clenobuterol hydrochloride colloidal gold detecting test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620019375 CN200944106Y (en) | 2006-03-23 | 2006-03-23 | Clenobuterol hydrochloride colloidal gold detecting test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN200944106Y true CN200944106Y (en) | 2007-09-05 |
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ID=38718743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620019375 Expired - Fee Related CN200944106Y (en) | 2006-03-23 | 2006-03-23 | Clenobuterol hydrochloride colloidal gold detecting test paper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN200944106Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102253211A (en) * | 2011-06-13 | 2011-11-23 | 清华大学深圳研究生院 | Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof |
-
2006
- 2006-03-23 CN CN 200620019375 patent/CN200944106Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102253211A (en) * | 2011-06-13 | 2011-11-23 | 清华大学深圳研究生院 | Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof |
| CN102253211B (en) * | 2011-06-13 | 2013-10-30 | 清华大学深圳研究生院 | Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |