CN1987402A - Multifunction quick dyeing liquid preparation and use for chest ascitic fluid and gynecologic exfoliated cell - Google Patents
Multifunction quick dyeing liquid preparation and use for chest ascitic fluid and gynecologic exfoliated cell Download PDFInfo
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- CN1987402A CN1987402A CN 200610104561 CN200610104561A CN1987402A CN 1987402 A CN1987402 A CN 1987402A CN 200610104561 CN200610104561 CN 200610104561 CN 200610104561 A CN200610104561 A CN 200610104561A CN 1987402 A CN1987402 A CN 1987402A
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Abstract
Structure of each exfoliated cell and pathogenic microorganism treated by the disclosed staining solution is sharp and easy to be identified. In one sheet of smear, not only figure of each exfoliated cell is displayed through one time of staining procedure, but also nucleus alloplasm cell, cancer cell, and pathogenic microorganism in each exfoliated cell can be displayed. Cell nucleus and cytoplasm are distinguished by red and blue colors under clear background through one time of staining procedure. Advantages are: less staining steps, easy of operation, simple preparing staining solution, smaller varieties of reagent needed, low cost, multiple uses of one staining solution, and high accuracy rate.
Description
One, denomination of invention: ascites pleural fluid, the multi-functional quick dye liquor preparation of gynaecology's cast-off cells and application
Two, affiliated technical field: the present invention relates to detection: various cause of diseases the support one's family inspection of thing, fungi and cancer cell and the evaluation of ovarian function in gynaecology's smear to various cast-off cells, atypical hyperplasia cell, dyskaryotic cell and cancer cell in ascites pleural fluid, sputum, the smear.
Three, background technology: various cast-off cells dyskaryotic cells of existing detection and BTR are compared with traditional dyeing method such as Gram, Wright's staining and HE, Pasteur etc., and dyeing procedure is numerous and diverse, dye liquor preparation is loaded down with trivial details, the dye liquor composition is numerous, detect that to dye positive rate low.Traditional decoration method can not be disposable with multiple pathogenic microorganism and cast-off cells dye, special project is specially dyed, it is single to dye the result; BTR (Zhuzhou health and happiness detectable company limited) need dye, its program is many, it is low to dye positive rate at twice though can do the dyeing of several bacteriums simultaneously on same smear; And clinical treatment has also been brought certain limitation.
Four, summary of the invention:
1, goal of the invention:
(1), this dye liquor be solved existing and traditional dye liquor in the dye liquor preparation loaded down with trivial details, the dye liquor composition is numerous, detect and dye the low defective of positive rate, improved the shortcoming that the result is comprehensive or special project is specially dyed, dyeing procedure is numerous and diverse that dyes of existing and traditional dye liquor existence comprehensively, can be disposable in same smear, cells such as various cast-off cells and various cancer cell, bacterium, mould, trichomonad be dyed, clear, the red indigo plant of its form sets each other off, distinct gorgeous, be convenient to observe and check; Detection dyes the positive rate height, and it is few to reach staining procedure, easy and simple to handle, time weak point, and the dye liquor preparation is simple, and required medicine is few, and long preservation is with low cost; Comprehensively, clear, accurate, detect and to dye the high purpose of positive rate.
(2), the difference of dyestuff forms and principle: this dye liquor is according to the intermolecular mutual suction-operated of traditional dyeing chemical reaction, various cells and bacterium, nuclear, slurry, with the difference of positive and negative electric charge and improving on the different theoretical foundation of used dyestuff acid, basic dyeing form.Originally dye the metachromatic principle that at first utilizes acid base indicator, its molecule is different with the structure of the ion that ionization is come out, so the color of molecule and ion is also different.In the solution of different PH, because the ratio of its molecular conecentration and ion concentration is different, therefore the color that demonstrates is also different.Add tertiary sodium phosphate in thionine, toluidine blue aqueous solution, along with the variation of solution acid-basicity, pH value increases.Add after the phosphoric acid trisodium in the solution, because negative ion (OH
-) the concentration increase, the kation (H that thionine, the ionization of toluidine blue aqueous solution generate
+) partly be neutralized H in the solution
+Reducing of concentration, OH
-The increase of concentration makes this dye liquor become pleochroism (especially when molten when alkaline) after thionine, toluidine blue solution and the tertiary sodium phosphate reaction, existing kation has negative ion again, just not only positively charged but also electronegative ion (zwitter-ion).Next utilizes protein is the characteristics that are the colloid thing of bronsted lowry acids and bases bronsted lowry both sexes, is with positive and negative different charge ion in the different solution of Acidity of Aikalinity, can be affine with dye liquor with different electric charges, form different colors.Make discriminating according to the various cell that dyes and the form and the color of pathogenic microorganism then.
2, technical scheme:
(1), finds that in practice some organic dyestuff can show different colors in different Acidity of Aikalinity solution.This dye liquor is according to the intermolecular mutual suction-operated of traditional dyeing chemical reaction, various cells and bacterium, nuclear, slurry, with the difference of positive and negative electric charge and improving on the different theoretical foundation of used dyestuff acid, basic dyeing form.Originally dye the metachromatic principle that at first utilizes acid base indicator, its molecule is different with the structure of the ion that ionization is come out, so the color of molecule and ion is also different.In the solution of different PH, because the ratio of its molecular conecentration and ion concentration is different, therefore the color that demonstrates is also different.Add tertiary sodium phosphate in thionine, toluidine blue aqueous solution, along with the variation of solution acid-basicity, pH value increases.Add after the phosphoric acid trisodium in the solution, because negative ion (OH
-) the concentration increase, the kation (H that thionine, the ionization of toluidine blue aqueous solution generate
+) partly be neutralized H in the solution
+Reducing of concentration, OH
-The increase of concentration, making this dye liquor become the existing kation of pleochroism after thionine, toluidine blue and the trisodium phosphate solution reaction has the just not only positively charged but also electronegative ion of negative ion (zwitter-ion) again. Next utilizes protein is the characteristics that are the colloid thing of bronsted lowry acids and bases bronsted lowry both sexes, is with positive and negative different charge ion in the different solution of Acidity of Aikalinity, can be affine with dye liquor with different electric charges, form different colors.Make discriminating according to the various cell that dyes and the form and the color of pathogenic microorganism then.
(2) composition of dye liquor:
Prescription: composition thionine, toluidine blue, tertiary sodium phosphate, water
Ratio is 1: 2: 1: 10
Preparing method:, fully shake up and then add that tertiary sodium phosphate mixes and dye liquor filtered and get final product with thionine, toluidine blue and after water mixes.
Use-pattern: various cast-off cells smears are treated that it slightly does the back and drip and go up this dye liquor 3-4 and drip abundant covering smear face, and washing with its flowing water after about 30 seconds to the 1 minute kinds is wettable microscopy.Need oily sem observation person to treat to add the cedar oil microscopy after smear is done.
(3), advantage: to compare its advantage as follows with traditional Gram, Wright's stain and existing BTR detectable:
The one, same this dye liquor of smear institute transfect cell background is very clear, and nucleus, slurry are once dyed, and red indigo plant is clearly demarcated, and the various cast-off cells that dyed, bacterium, the vivid form of fungi color are clear, are similar to a colour picture; And how unintelligible the background that goes out with traditional dyeing liquor is, nuclear, slurry is smudgy and dyeing procedure is complicated; No matter be traditional dyeing liquor or BTR, they all are that nuclear, slurry must dye at twice; For the first time earlier with after the nuclear staining, for the second time could pulp is outstanding; And can not use by a liquid more.
The 2nd, the positive rate that same patient, same smear dye out with this dye liquor is higher than traditional dyeing liquid and dyes positive rate 60%; Be higher than that BTR dyes 26%;
3, beneficial effect:
Gynaecology generally investigates 2801 examples, every example is manufactured 2 on smear, dye 1 with BTR, dye 1 with this dye liquor, comparing result: BTR dyes and detects positive findings 306 examples, recall rate 10.05%, this dye liquor dye positive findings and detect 892 examples, recall rate 37.2%; Wherein to dye the mould recall rate be 21.5% to BTR, and it is 42.8% that this dye liquor dyes the mould recall rate; It is 15.5% that BTR dyes the trichomonad recall rate, and it is 28.8% that this dye liquor dyes the trichomonad recall rate; BTR dyes dyskaryotic cell 0 example, and this dye liquor dyes dyskaryotic cell 7 examples.It is extremely remarkable that the two compares difference.
The various cells that dye and the form of pathogenic microorganism:
(1), light blue, the nuclear of epithelial cell, the endochylema in hydrothorax, the ascites is aubergine or mazarine; The sputum smear, endochylema is pink or light blue, nuclear presents aubergine; Cancer cell, endochylema are light blue, and nuclear presents dark conspicuous look or aubergine, the big slurry of nuclear, and few form is very easy distinguishes.
(2) form of various pathogenic microorganisms in gynaecology's secretion:
Trichomonad resembles a pear in shape, oval or irregular shape, that characteristics are that slurry is is light blue or sky blue, nuclear for mazarine be jujube nuclear shape or fusiformis and off normal, have be aubergine, behind the degeneration necrosis, dyed and present aubergine or pink and form and make the result;
Fungal hyphae is dyed mazarine and is tangible dendroid or match stick sample, and visible its garden shape or the melon seeds shape mazarine spore that has adheres to, form is very clear;
The cilium bacterium is that typical hair is thread, thickness is consistent, different in size, appearance in heaps sometimes, presents aubergine or sky blue;
Be everlasting between cell slabbing and heap shape shape of Gartner Salmonella distributes, in be light blue under times mirror or aubergine, little garden shape, point-like, grains of sand shape; It is little shaft-like that the high power lens lower part is;
Clues cell is full of in its epithelial cell that comes off slurry that a large amount of Jia Dena Schwann Cells is for clues cell, its form is clear easily distinguishes;
Gonococcus sees in the leucocyte slurry and outside the slurry in high power lens or its characteristics of oily mirror obviously mazarine or aubergine garden shape, the symmetrical distribution of kidney shape;
Lactobacillus is mazarine or aubergine, different in size, the appearance in heaps sometimes of thin rod shape; Leucocyte nuclear is double leaf shape and lobulated mazarine or aubergine, and it is light blue that endochylema is;
The consistent garden of red blood cell size and form shape structure, light blue, the interior sky of nuclear membrane are bright, mucus is sky blue;
(3), gastric mucosa helicobacter dyeing presents arc or S shape is purplish red or sky blue under high power lens.
4, embodiment:
With thionine, toluidine blue and after water mixes, fully shake up and then add that tertiary sodium phosphate mixes and dye liquor filtered and get final product.Various cast-off cells smears are treated that it slightly does the back and drip and go up this dye liquor 3-4 and drip abundant covering smear face, and washing with its flowing water after about 30 seconds to the 1 minute kinds is wettable microscopy.Need oily sem observation person to treat to add the cedar oil microscopy after smear is done.This dye liquor economy, easy, quick, comprehensive, clear, accurate detects and dyes the positive rate height; In ascites pleural fluid, sputum and gynaecology's smear, in same smear, not only can dye the form of various cast-off cells and can dye dyskaryotic cell and cancer cell in the various cast-off cells, and can once dye various cast-off cells and pathogenic microorganism.Nucleus, slurry are once dyed.
Claims (3)
1, a kind of ascites pleural fluid, the multi-functional quick dye liquor quick multifunctional dye liquor preparation of gynaecology's cast-off cells and application, with thionine, toluidine blue and after water mixes, fully shake up add then that tertiary sodium phosphate mixes and dye liquor filtered after, various cast-off cells smears are treated that it slightly does the back and drip and go up this dye liquor 3-4 and drip abundant covering smear face, and washing with its flowing water after about 30 seconds to the 1 minute kinds is wettable microscopy.It is characterized in that staining procedure is few, easy and simple to handle, time weak point, the dye liquor preparation is simple, and required medicine is few, and long preservation is with low cost; Comprehensively, clear, accurate, detect and to dye the positive rate height;
2, it is characterized in that at ascites pleural fluid, reach in gynaecology's smear according to the described multi-functional quick dye liquor of claim, in same smear, not only can dye the form of various cast-off cells and can dye dyskaryotic cell and cancer cell in the various cast-off cells, and can once dye various cast-off cells and pathogenic microorganism.
3, it is characterized in that at thionine, toluidine blue according to the described multi-functional quick dye liquor of claim and after water mixes, the adding tertiary sodium phosphate can improve the potential of hydrogen of dye liquor, thereby fully combine with the cast-off cells and the pathogenic microorganism that are dyed, the nucleus of cast-off cells and pathogenic microorganism, the potential of hydrogen of slurry are changed, make nucleus, that slurry once dyes and examine, starches red indigo plant is clearly demarcated.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101045611A CN100561173C (en) | 2006-09-09 | 2006-09-09 | Ascites pleural fluid, the multi-functional quick dye liquor preparation of gynaecology's cast-off cells and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101045611A CN100561173C (en) | 2006-09-09 | 2006-09-09 | Ascites pleural fluid, the multi-functional quick dye liquor preparation of gynaecology's cast-off cells and application |
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|---|---|
| CN1987402A true CN1987402A (en) | 2007-06-27 |
| CN100561173C CN100561173C (en) | 2009-11-18 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
| CN107502649A (en) * | 2017-09-26 | 2017-12-22 | 中国食品药品检定研究院 | A kind of new quick stain for cell and its application |
-
2006
- 2006-09-09 CN CNB2006101045611A patent/CN100561173C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
| CN103323313B (en) * | 2012-03-23 | 2016-06-29 | 黄伏生 | Rui Shi method is for the serous cavity exfoliative cyte colouring method of liquid basal cell instrument |
| CN107502649A (en) * | 2017-09-26 | 2017-12-22 | 中国食品药品检定研究院 | A kind of new quick stain for cell and its application |
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| Publication number | Publication date |
|---|---|
| CN100561173C (en) | 2009-11-18 |
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