CN1986820A - Alcohol output increasing method and device - Google Patents
Alcohol output increasing method and device Download PDFInfo
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- CN1986820A CN1986820A CNA2006101250739A CN200610125073A CN1986820A CN 1986820 A CN1986820 A CN 1986820A CN A2006101250739 A CNA2006101250739 A CN A2006101250739A CN 200610125073 A CN200610125073 A CN 200610125073A CN 1986820 A CN1986820 A CN 1986820A
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Abstract
The present invention relates to alcohol producing fermentation process and apparatus with starch as material. After biological enzyme active catalyst on carrier is set inside some container, turbid liquid with starch is made to stay in the container to contact with the catalyst for 30-60 min. The biological enzyme active catalyst includes pectinase in 8-15 wt%, cellulase in 10-20 wt% and trace elements, and the carrier is porous material, with the pectinase and cellulase being obtained through strain fermentation. The present invention has increased alcohol yield.
Description
Technical field
The present invention relates to a kind of is the method and apparatus that fermenting raw materials is produced alcohol with starch.Particularly on the basis of traditional biomass to alcohol conversion process and equipment, adopt biotechnology to improve the method and apparatus of Alcohol Production rate greatly.
Background technology
At present, about 3,000,000 tons of China's alcohol annual production is mainly based on fermentative Production.Along with the increasing of expanding economy, progress of science and technology and scientific research investment dynamics, though aspects such as the Alcohol Production technology is continuously fermented at low temperature boiling, living starch alcohol fermentation, immobilized cell, seed selection high temperature resistant distillery yeast, differential distillation obtain encouraging success; But, seriously restricted the development of China Alcohol Production enterprise owing to problems such as production technology level fall behind, the alcohol the yield of liquor is low, the comprehensive utilization of resources rate is not high, environmental pollution is serious.
Be to improve the Alcohol Production yield, reduce energy consumption, the water consumption of Alcohol Production process, the protection environment is fully used resource, to existing biomass to alcohol conversion process and equipment suitably adjust improve imperative.There is document openly to be reported in the fermentation system except adding yeast in recent years, add some active complex enzymes, for example amylase, cellulase, aspartic protease, polygalacturonase etc., special metabolic mechanism by active complex enzyme, improve the zymamsis environment, improve the smart content of fermenting-ripening raw spirit, thereby reach the raising alcohol output, cut down the consumption of energy, reduce the purpose of environmental pollution.Through retrieval, we have found some following Chinese patent literatures:
1, [autograph] fuel alcohol preparation research [author] Zhang Xuxia Dong Hai continent Food Science and Engineering institute of [mechanism] Shandong Agricultural University, [periodical name] grain and grease .2006 (6) .-22-24[digest] this research prepares on the fuel alcohol basis at traditional zymotic, according to material composition with influence fermentation factor, interpolation polygalacturonase and phytase in experiment; The result shows: add polygalacturonase 6U/g raw material, action time 30min, during phytase 7U/g raw material, make alcoholic strength improve 1.6% (v/v), liquor ratio of raw material improves 3.9%, reduces production costs 4.3%, shorten fermentation time 8hr simultaneously, produce the fuel alcohol cost thereby effectively reduce; Economic and Efficiency Analysis shows: this is a kind of practical fuel alcohol preparation technology.
2, the interpolation of [autograph] zymin is to influence [author] Wang Jiang ripple Xue petrel Zou Yu tinkling of pieces of jade reverb China [mechanism] Hubei University Of Technology of early long-grain nonglutinous rice raw material fermentation production alcohol, and .2005 (4) .-15-17[digest is brewageed by [periodical name] China] early long-grain nonglutinous rice raw material fermentation production alcohol is studied.The result shows: the interpolation of amylase, cellulase, aspartic protease, polygalacturonase does not have positive effect to zymamsis.The saccharifying enzyme optimum addition is the 225U/g raw material, and best solid-liquid ratio is 1: 3.5, and 300 ℃ of 120h, alcoholic strength reach 9.0% (v/v), and residual total reducing sugar is 1.20%, and the rate of getting alcohol reaches 51.28%.
3, development [author] Hu Xuezhi Wei Cheng in the morning Zhe Liping of [autograph] efficient zymamsis agent does single upright bio-engineering research center, [mechanism] Chinese Academy of Sciences Shanghai, [periodical name] Jiangsu seasoning nonstaple food .2005,22 (1) .-39-42[digests] to have introduced be that raw material is when carrying out zymamsis with starch, make protein decomposition in the raw material by adding aspartic protease, and impel yeast growth, shorten fermentation period, thereby create conditions for making efficient zymamsis agent.Select the mutant strain 537 of Aspergillus usamii M90 to be the strain of setting out, the experiment of employing plate method, optimize the M90-537-42 acid protease activity that filters out and reach 15000~17000u/g (dry), and contain the lytic enzymes such as zytase, cellulase, polygalacturonase and saccharifying enzyme of greater activity.Test by zymamsis, determining to add 0.1% combination of acidic proteolytic enzyme wheat bran can make alcohol output increase by 2%, the active dry yeast mixing that adds equivalent promptly constitutes efficient zymamsis agent, compared with the control, fermentation raw spirit concentration can improve about 2%, liquor ratio of raw material can improve 5%, and economic benefit is fairly obvious.
4, [autograph] polygalacturonase G5512 bacterial strain deep fermentation pilot scale and Study on extraction [author] She Qiusheng [1] Guo Aiguang [1] Wang Jianlin [2] Shao Jianning [3] Chao open life science institute of [1] bang gloomy [3] [mechanism] [1] Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology, [2] Lanzhou University's life science institute, [3] Institute of Biology, Gansu Academy of Sciences, [periodical name] journal .2004 of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology, 32 (5) .-85-88[digests] serve as to produce bacterium with the high yield polygalacturonase mutant strain G 5512 of carbon black aspergillus (Aspergillus carbonarius) AS3.396, in the 500L fermentor tank, carry out the deep fermentation pilot scale.The result shows, carries out feed supplement during the fermentation respectively and regulates pH, can increase substantially enzymic activity; Carry out feed supplement simultaneously and regulate pH, the enzymatic activity high of fermented liquid can reach 1248.17U/mL when being substrate with the high ester pectin, reaches 2235.75U/mL with the low-ester pectin during for substrate, has improved about 2 times and 11 times than starting strain respectively.By test, also determined the best processing route of separation and Extraction polygalacturonase from fermented liquid: fermenting enzyme liquid → ultrafiltration → alcohol precipitation → vacuum-drying, its enzymic activity rate of recovery is reaching 89.3% with the high ester pectin during for substrate, is 80.3% with the low-ester pectin during for substrate.
5, application [author] Zhang Qiang [1] ground force [1] Hou Lin [1] golden flower [1] Piao Jinghui [1] Ling Feng [2] Li Hong of [autograph] fermentation intensifier in Alcohol Production comes [2] Yu Xiurong [2] [mechanism] [1] Jilin Light Industrial Design Inst., [2] sky, Jilin Province closes grain distillery, [periodical name] brewing science and technology .2004 (2) .-54-57[digest] in Alcohol Production, add 5 kinds of fermentation intensifiers such as polygalacturonase, can significantly improve liquor ratio of raw material, fermentation time shortens 5h, reduces production costs about 3.2%.Test shows that the optimum addition of 5 kinds of fermentation intensifiers is respectively: (1) polygalacturonase 12u/g, when raw materials pretreatment, to add, and action time, mash viscosity can fall in 1h.(2) cellulase 50u/g joins in the liquefied pot, can reduce liquefying time greatly.(3) phytase 8u/g adds in fermentation.(4) aspartic protease 15u/g adds when the fermentation beginning.(5) gram bacterium spirit 6mg/g adds at earlier fermentation.
6, the biotechnology institutes of [mechanism] Wuxi Light Industry Univ. such as research [author] Wang Xiao rosy clouds Zhang Kechang that polygalacturonase is used in [autograph] alcohol high concentrated fermentation process, [periodical name] food and fermentation industries .2001,27 (3) .-44-47[digests] viscosity of karusen is studied when utilizing polygalacturonase to reduce sweet potato raw material alcohol high concentrated fermentation.Experimental result shows: different material-water ratios, the consumption difference of the required polygalacturonase of reduction mash viscosity.Material-water ratio is 1: 2.5 o'clock, (consumption is that 8u/g (raw material) is at 45 ℃ of following enzymolysis feed liquid 1h to polygalacturonase, the viscosity of liquefaction back mash can reduce by 55.26%, and the consumption of α-Dian Fenmei does not have influence substantially to the back mash viscosity that liquefies, and adds the polygalacturonase depolymerized pectin and should carry out before liquefaction.
7, the solid-state zymamsis of [autograph] cellulose waste residue and Mierocrystalline cellulose-starch research [author] Du of fermenting altogether grasps extra large Qu Yinbo [mechanism] not quite clear [periodical name] food and fermentation industries .1995 (5) .-15-20[digest] this paper studies the solid-state technology of alcohol of paper mill cellulose waste residue.Test-results shows, utilize cellulase song and alcohol active dried yeast, adopt solid state synchronous diastatic fermentation technology and feed supplement technology, be the 20IU/g substrate at the filter paper enzyme activity consumption, add as under 4.35 ℃ of conditions, wine unstrained spirits wine Du Keda 5.1 (V/w), the paper mill carefully spirit yield of assorted fiber is 0.17 (w/w).
8, application progress [author] Liu Ming Ni Hui Wu Yong abundant [mechanism] the Collects The American University biotechnology institute of [autograph] cellulase in alcohol industry, [periodical name] brewing science and technology .2006 (7) .-83-85, the 90[digest] cellulase (Cellulase) be meant can hydrocellulose β-1,4 glucoside bonds, make Mierocrystalline cellulose become the general name of one group of enzyme of cellobiose and glucose, the induction type prozyme of being made up of endoglucanase, dextran excision enzyme, 3 main components of beta-glucosidase is.When zymamsis, add cellulase can significantly improve the yield of liquor of alcohol and liquor and utilization ratio of raw materials, reduction feed liquid viscosity, shorten fermentation time; Utilize cellulase with cellulosic material produce fuel alcohol effectively alleviating energy crisis, alleviate environmental pollution and solution wasting of resources problem.
9, development [author] Hu Xuezhi Wei Cheng in the morning Zhe Liping of [autograph] efficient zymamsis agent does single upright bio-engineering research center, [mechanism] Chinese Academy of Sciences Shanghai, [periodical name] Jiangsu seasoning nonstaple food .2005, it is being that raw material is when carrying out zymamsis with starch that 22 (1) .-39-42 have introduced, make protein decomposition in the raw material by adding aspartic protease, and impel yeast growth, shorten fermentation period, thereby create conditions for making efficient zymamsis agent.Select the mutant strain 537 of Aspergillus usamii M90 to be the strain of setting out, the experiment of employing plate method, optimize the M90-537-42 acid protease activity that filters out and reach 15000~17000u/g (dry), and contain the lytic enzymes such as zytase, cellulase, polygalacturonase and saccharifying enzyme of greater activity.Test by zymamsis, determining to add 0.1% combination of acidic proteolytic enzyme wheat bran can make alcohol output increase by 2%, the active dry yeast mixing that adds equivalent promptly constitutes efficient zymamsis agent, compared with the control, fermentation raw spirit concentration can improve about 2%, liquor ratio of raw material can improve 5%, and economic benefit is fairly obvious.
10, the biotechnology institutes of [mechanism] Wuxi Light Industry Univ. such as research [author] Wang Xiao rosy clouds Zhang Kechang that polygalacturonase is used in [autograph] alcohol high concentrated fermentation process, [periodical name] food and fermentation industries .2001,27 (3) .-44-47[digests] viscosity of karusen is studied when utilizing polygalacturonase to reduce sweet potato raw material alcohol high concentrated fermentation.Experimental result shows: different material-water ratios, the consumption difference of the required polygalacturonase of reduction mash viscosity.Material-water ratio is 1: 2.5 o'clock, (consumption is that 8u/g (raw material) is at 45 ℃ of following enzymolysis feed liquid 1h to polygalacturonase, the viscosity of liquefaction back mash can reduce by 55.26%, and the consumption of α-Dian Fenmei does not have influence substantially to the back mash viscosity that liquefies, and adds the polygalacturonase depolymerized pectin and should carry out before liquefaction.
11, Chinese patent, application (patent) number: 01133450.9 applying date: 2001.11.16 title: fermentation synergist for fuel alcohol application (patent right) people: Jilin Light Industrial Design Inst. address: No. 18, Gongnongda Road, Changchun City, Jilin Province, summary: the present invention relates to a kind of fermentation synergist for fuel alcohol, belong to the corn deep processing technology field.In raw material processing and fermenting process, add toughener, this toughener is made up of following product: polygalacturonase 8-12u/g raw material, cellulase 5-10u/g raw material, hemicellulase 6-8u/g raw material, zytase 4-8u/g raw material, phytase 1-4u/g raw material, aspartic protease 5-20u/g raw material, bacteria protease 2-6u/g raw material, fungal proteinase 2-6u/g raw material, microbiotic 0.1-1ppm, advantage of the present invention is: the efficient composite enzyme that utilizes complicated enzyme system and microorganism to constitute, make saccharification and fermentation process parallel, and the alcoholic strength in the fermentation liquid improves.After adopting this prozyme, can make full use of the various nutritive substances in the raw material, improve more than the alcoholic strength to 20% (v/v), shorten fermentation period, save the investment of the energy and sacchariferous equipment, promote further developing of fuel alcohol.Claims 1, a kind of fermentation synergist for fuel alcohol is characterized in that: this toughener is grouped into by following one-tenth: (a) polygalacturonase 8-12u/g raw material, and cellulase 5-10u/g raw material, (b) aspartic protease 5-20u/g raw material, (c) microbiotic 0.1-1ppm,
12, Chinese patent, application (patent) number: 200410034454.7 applyings date: 2004.04.12 title: the special-purpose saccharification nourishing complex enzyme of alcohol and alcohol fuel and use application (patent right) people: Guo Feng address: Room 105, No. 7 building, Pudong New Area, Shanghai Zhang Yanglu 628 lanes, summary: the present invention relates to a kind of alcohol and the special-purpose saccharification nourishing complex enzyme of alcohol fuel, it is the prozyme that the one-tenth by following weight per-cent is grouped into: complex cellulase: 10-20%, polygalacturonase: 3-7%, proteolytic enzyme: 5-15%, debranching factor: 5-15%, phytase: 5-15%, saccharifying enzyme: surplus.Of the present inventionly be applied in disposable described saccharification nourishing complex enzyme is added into of saccharification stage and treat in the saccharification material.The present invention is a plurality of enzymes preparation compound prescription, and the saccharifying enzyme in the alternative existing technology reduces production costs; The present invention substitutes nutritive salt with zymin, reduces acidity, reduces assorted bacterium, accelerates fermenting speed; The present invention becomes repeatedly to be added to once and adds in use, and operation simplifies the operation.The special-purpose saccharification nourishing complex enzyme of 1. 1 kinds of alcohol of principal claim and alcohol fuel is characterized in that it is the prozyme that the one-tenth by following weight per-cent is grouped into: complex cellulase: 10-20% polygalacturonase: 3-7% proteolytic enzyme: 5-15% debranching factor: 5-15% phytase: 5-15% saccharifying enzyme: surplus.
13, Chinese patent, application (patent) number: the 200510032411.X applying date: 2005.11.16 title: a kind of with the prozyme of brewing wine with raw materials and method application (patent right) people: the Fu Zhongxiong that makes wine with this prozyme, address: in Hunan Province's Yongzhou City Hu'nan University of Science and Engineering, summary: a kind of with the prozyme of brewing wine with raw materials and the method for making wine with this prozyme, this prozyme can transform saccharifying enzyme by height, the wine high activity dried yeast, three kinds of one-tenth of polygalacturonase are grouped into, their weight percent is followed successively by 45%-67%, 27%-45%, 4-12%.If add purebred head mold song on this basis again, then the effect of wine brewing can be better.Make this prozyme, only need take by weighing each component in proportion, stir and get final product.Make wine with this prozyme, only need raw material pulverizing or pulp, by per 100 kilograms of dry product raw material, the prozyme of 500-1000 gram stirs and sends into the interior fermentation of synchronous fermentor tank 80-140 hour, distills to obtain wine or alcohol again.Implement the present invention, not only can be with various cereal gramineous as giving birth to the wine raw material, each potato seed class of can also China abounding with wine raw material of making a living, enlarged the raw material source, save factory building, reduced cost, also can realize the generation that recycles and stop to pollute of raw material.Principal claim: 1, a kind of prozyme with brewing wine with raw materials is characterized in that the moiety of this prozyme and the weight percent of each component are: high saccharifying enzyme 45%-67%, wine high activity dried yeast 27%-45%, the polygalacturonase 4-12% of transforming.
Summary of the invention
The inventor is through the research and the test of hardships for many years, a kind of outside release active catalyst that does not stop by the bioenzyme activity support of the catalyst has been finished in research, by pectin, hemicellulose and the Mierocrystalline cellulose in the active biological enzyme effect starch-splitting, promote starch to transform; The active biological enzyme effect suppresses assorted bacterium and generates, and improves yeasting, thereby improves fermentation efficiency.
The method of raising alcohol output of the present invention, be that carrier with the bioenzyme activity catalyzer is placed in the container, give the starch troubled liquor enter before the fermentor tank this container of flowing through, and stopped 30-60 minute, it is fully contacted, described bioenzyme activity catalyzer is polygalacturonase, cellulase and trace element, and described carrier adopts porous class material.
Above-described polygalacturonase (pectinex) accounts for the 8-15% of the quality percentage composition of whole active catalyst, the quality percentage composition that Mierocrystalline cellulose (Cellulose) accounts for whole active catalyst is 10-20%, described trace element is the water-soluble inorganic salt of manganese, iron, zinc, antimony and tin, for example vitriol, nitrate, chlorate.In these water-soluble inorganic salts, manganese salt accounts for the 0.5-2% of active catalyst quality percentage composition; Molysite accounts for the 2-5% of active catalyst quality percentage composition; Zinc salt accounts for the 1-2% of active catalyst quality percentage composition; Antimonic salt accounts for the 0.2-1% of active catalyst quality percentage composition; Pink salt accounts for the 0.3-1 of active catalyst quality percentage composition.All the other are carriers, and carrier adopts the sugarcane blob of slag of compacting, multilayer net that sisal fibers is made into or with being filled into pottery, baton round and net, and ceramic, baton round is surperficial aperture.
Above-described polygalacturonase and cellulase can be bought from microbial product company, also can cultivate generation from microbial strains.Some open source literatures are introduced, and polygalacturonase can obtain with strain fermentation, and microorganism polygalacturonase source is extremely extensive, comprises bacterium, fungi, actinomycetes. Lus), Penicillium (Penicillium), Sporotrichum (Sporotrichum), Sclerotinia (Sclerotinia) etc., polygalacturonase generally forms through these bacterial classification liquid submerged fermentations are refining.Described cellulase also is to obtain with strain fermentation, the microorganism that is used for the production of cellulose enzyme is also more, wherein the bacterial classification that enzyme activity is stronger is a wood mould (Trichodermasp), whiterot fungi (Panus conchatu.), aspergillus (Aspergillus sp.), head mold (Rhizopus) and Penicillium (Penicillium), with Trichodermareesei (Trichoderma reesei Rut C-30), viride (TrichodermavirideAS3.3032), koning trichoderma (Trichoderma Koningii), crassas (Neurospora sp.) etc. are the typical case the most, it is the bacterium of cellulase production preferably of generally acknowledging at present, and made preparation, the document introduction is arranged, black-koji mould (Aspergilusniger, AN) has the effect of producing polygalacturonase and cellulase simultaneously, so can be with black-koji mould (Aspergilus niger, AN), obtain being fit to the bacterial classification of various starch through domestication.But if these bacterial classifications without domestication, just can not be survived in starch liquid, and nutrition also is unfavorable for growth inadequately, more can not talk about the effect of coordinating distillery yeast.
The bacterial classification that produces polygalacturonase and cellulase generally will add substratum earlier to carry out enlarged culturing 1-2 days in 25-35 ℃ greenhouse, substratum adopts starch+peptone+sucrose or dextrose culture-medium commonly used in the document, general 1 liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, cultivating the back adding tamed 1 day according to the yellow seriflux of fermentation concentration again, (temperature and fermentation jar temperature are basic identical), the growing state of observing that group bacterial classification of that class preferably just preferably comes out, bacterial classification and enzyme liquid with domestication is filled into carrier together and is placed in the tank body then, so just can produce.
The present invention was introduced into yellow seriflux the bioenzyme activity catalyst-assembly before this before the alcohol amylofermentation, flow through behind the bioenzyme activity promotor carrier, make yellow seriflux fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, makes it to change into five-carbon sugar and hexose.Active biological enzyme suppresses assorted bacterium by biological enzyme and hydrolysis acid solution and generates along with material enters saccharification, the continuation effect of fermentation workshop section; Promotor enters yeast cell nuclear and activates saccharomycetic physiologically active, and metabolism improves fermentation efficiency, improves the ethanol production effect thereby reach.
Technical characterstic of the present invention is: because of not needing to increase starting material, need not increase power and steam, so come any operation negative pressure for the original production tape; Present technique can be eliminated pectin and the assorted bacterium that is unfavorable for yeasting, and starch effectively is converted into sugar, improves fermentation efficiency, effectively improves ethanol production.Therefore, use present technique not only economy but also practicality.
The principle that technical scheme of the present invention is achieved is: by polygalacturonase, the biological activity promoters that cellulase and trace element are formed is placed to the front of fermentor tank with carrier, making starchy material be in contact with it the back improves active, be swift in response, create the yeast fermentation good environment, though the document of prior art is also mentioned polygalacturonase, cellulase can improve the yield of liquor and the utilization ratio of raw materials of alcohol, reduce the viscosity of feed liquid, shorten fermentation time, but owing to do not adopt immobilized form, do not add trace element in the material yet, reaction process is discontinuous, and effect is remarkable inadequately.So gordian technique of the present invention is under the effect of biological enzyme, micro-compound active catalyst, can destroy colloid in the cassava mash, part decomposition of cellulose and hemicellulose, promote Mierocrystalline cellulose, hemicellulose to decompose, the synergy of enzyme suppresses varied bacteria growing during the fermentation, realize creating good yeasting and yeast and utilize substrate, improve ethanol production.
Since the test of Pingguo, Guangxi grain distillery, small test moved for some time, produces the alcohol rate and improved 5% to 15% on former basis from the yellow Rong Sheng of contriver in 2000 in the present invention.
21 century is the fiercest century of energy contention, and countries in the world all tap a new source of energy when grabbing the existing energy energetically.Therefore develop a kind of new wide material sources, the cheap energy has been very urgent.The U.S. just has realized that the advantage of alcohol fuel as far back as eighties of last century, forces to add 10% alcohol in fuel, and has added 22% in Brazil.Project on China Jilin, Henan, Anhui and other places with the alternative gasoline of alcohol alcohol has also had much effect; 2008 Beijing Olympic, Beijing Municipal Government also promised to undertake city public transport and taxi use instead alcohol as clean fuel to reduce atmospheric pollution.And grain resource is also more and more in short supply, and in this case technology of the present invention being used to produce alcohol is matters vital to national well-being and the people's livelihood, and prospect is boundless.So after the present invention is widely used in the factory of starch Alcohol Production, under the situation of flow process that does not change the original production line and equipment, can improve ethanol production greatly, for transport trade provides more multipotency source.
Description of drawings
Fig. 1 is that biological activity promoters of the present invention is implemented structure iron.
As shown in the figure, material enters system from feed pipe 4, can be divided into two-way, can enter, then from flowing to tank body down by the bottom of first tank body 3, through porous class material support 3, flow out from first tank body top, the bottom that enters second tank body enters, equally from flowing to tank body down, through porous class material support 3, export to fermentor tank and ferment.
Embodiment
With black-koji mould (Aspergilus niger, AN) adding earlier substratum is put into 25-35 ℃ greenhouse and cultivated 1-2 days, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also to tame 1 day conditions such as (constant) temperature again after the cultivation according to the yellow seriflux of fermentation concentration, culturing process preferably adds the water-soluble inorganic salt of manganese, iron, zinc, antimony and tin, and add-on manganese salt accounts for the 0.5-2% of substratum quality percentage composition; Molysite accounts for the 2-5% of substratum quality percentage composition; Zinc salt accounts for the 1-2% of substratum quality percentage composition; Antimonic salt accounts for the 0.2-1% of substratum quality percentage composition; Pink salt accounts for the 0.3-1 of substratum quality percentage composition, enlarge 30 kilograms of the fermented products that obtain after domestication is cultivated (comprise domestication after bacterial classification and enzyme liquid), add 1 kilogram of manganous sulfate, 3 kilograms in ferrous sulfate, 1 kilogram in zinc sulfate, 0.2 kilogram of 0.3 kilogram of antimony trisulfate and tin protochloride mix with 80 kilograms of bagasse and are pressed into cake, be placed in the tank body, yellow seriflux enters the bagasse of laying the bioenzyme activity catalyzer, starch flow is behind bioenzyme activity promotor carrier, fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, and fermentation alcohol can improve output 5%.
With genus kluyveromyces (Kluyveromyces), koning trichoderma (Trichoderma Koningii) adds earlier substratum respectively and is put into 25-35 ℃ greenhouse and cultivated 1-2 days, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also to tame again 1 day after the cultivation according to the yellow seriflux of fermentation concentration, (condition such as temperature is constant) culturing process preferably adds manganese, iron, zinc, the water-soluble inorganic salt of antimony and tin, add-on manganese salt accounts for the 0.5-2% of substratum quality percentage composition; Molysite accounts for the 2-5% of substratum quality percentage composition; Zinc salt accounts for the 1-2% of substratum quality percentage composition; Antimonic salt accounts for the 0.2-1% of substratum quality percentage composition; Pink salt accounts for the 0.3-1 of substratum quality percentage composition, get each and enlarge 20 kilograms of domestication cultivation and fermentation things (comprise domestication after bacterial classification and enzyme liquid), add 1 kilogram of manganous sulfate, 3 kilograms in ferrous sulfate, 1 kilogram in zinc sulfate, 0.2 kilogram of 0.3 kilogram of antimony trisulfate and tin protochloride and 80 kilograms of wooden chaffs, install in the hollow ceramic ball (appearance has many apertures), be placed in the tank body, yellow seriflux enters lays bioenzyme activity cat ceramic ball, starch flow is behind bioenzyme activity promotor carrier, fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, and outlet can improve 5% to 15%.
With erwinia (Erwinia), Trichodermareesei (Trichoderma reesei Rut C-30) adds earlier substratum respectively and is put into 25-35 ℃ greenhouse and cultivated 1-2 days, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also to tame again 1 day after the cultivation according to the yellow seriflux of fermentation concentration, (condition such as temperature is constant) culturing process preferably adds manganese, iron, zinc, the water-soluble inorganic salt of antimony and tin, add-on manganese salt accounts for the 0.5-2% of substratum quality percentage composition; Molysite accounts for the 2-5% of substratum quality percentage composition; Zinc salt accounts for the 1-2% of substratum quality percentage composition; Antimonic salt accounts for the 0.2-1% of substratum quality percentage composition; Pink salt accounts for the 0.3-1 of substratum quality percentage composition, get each and enlarge 25 kilograms of domestication cultivation and fermentation things (comprise domestication after bacterial classification and enzyme liquid), add 1 kilogram of manganous sulfate, 2.5 kilograms in ferrous sulfate, 1 kilogram in zinc sulfate, 0.3 kilogram of 0.2 kilogram of antimony trisulfate and tin protochloride and 80 kilograms of wooden chaffs, install in the hollow baton round (appearance has many apertures), be placed in the tank body, yellow seriflux enters the baton round of laying the bioenzyme activity catalyzer, starch flow is behind bioenzyme activity promotor carrier, fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, and outlet can improve 5% to 15%.
With viride (Trichoderma virideAS3.3032), Spirillospora (Spirillospora) expands respectively and adds earlier substratum and be put into 25-35 ℃ greenhouse and cultivated 1-2 days, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also to tame again 1 day after the cultivation according to the yellow seriflux of fermentation concentration, (condition such as temperature is constant) culturing process preferably adds manganese, iron, zinc, the water-soluble inorganic salt of antimony and tin, add-on manganese salt accounts for the 0.5-2% of substratum quality percentage composition; Molysite accounts for the 2-5% of substratum quality percentage composition; Zinc salt accounts for the 1-2% of substratum quality percentage composition; Antimonic salt accounts for the 0.2-1% of substratum quality percentage composition; Pink salt accounts for the 0.3-1 of substratum quality percentage composition, get each and enlarge 30 kilograms of domestication cultivation and fermentation things (comprise domestication after bacterial classification and enzyme liquid), add 1 kilogram of manganous sulfate, 3 kilograms in ferrous sulfate, 1 kilogram in zinc sulfate, 0.2 kilogram of 0.3 kilogram of antimony trisulfate and tin protochloride mix with the multilayer net that 80 kilograms of sisal fiberss are made into and are pressed into cake, be placed in the tank body, yellow seriflux enters lays the multilayer net that bioenzyme activity catalyzer sisal fibers is made into, starch flow is behind bioenzyme activity promotor carrier, fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, and outlet can improve 5% to 15%.
With Sclerotinia (Sclerotinia), whiterot fungi (Panus conchatu.) adds earlier substratum respectively and is put into 25-35 ℃ greenhouse and cultivated 1-2 days, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also to tame again 1 day after the cultivation according to the yellow seriflux of fermentation concentration, (condition such as temperature is constant) culturing process preferably adds the water-soluble inorganic salt of manganese, iron, zinc, antimony and tin, and add-on manganese salt accounts for the 0.5-2% of substratum quality percentage composition; Molysite accounts for the 2-5% of substratum quality percentage composition; Zinc salt accounts for the 1-2% of substratum quality percentage composition; Antimonic salt accounts for the 0.2-1% of substratum quality percentage composition; Pink salt accounts for the 0.3-1 of substratum quality percentage composition, get each and enlarge 30 kilograms of domestication cultivation and fermentation things (comprise domestication after bacterial classification and enzyme liquid), add 1 kilogram of manganous sulfate, 3 kilograms in ferrous sulfate, 1 kilogram in zinc sulfate, 0.2 kilogram of 0.3 kilogram of antimony trisulfate and tin protochloride mix with the multilayer net that 80 kilograms of sisal fiberss are made into and are pressed into cake, be placed in the tank body, yellow seriflux enters lays the multilayer net that bioenzyme activity catalyzer sisal fibers is made into, starch flow is behind bioenzyme activity promotor carrier, fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, and outlet can improve 5% to 15%.
Embodiment 6
With Spirillospora (Spirillospora), crassa (Neurospora sp.) adds earlier substratum respectively and is put into 25-35 ℃ greenhouse and cultivated 1-2 days, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also to tame again 1 day after the cultivation according to the yellow seriflux of fermentation concentration, (condition such as temperature is constant) culturing process preferably adds the water-soluble inorganic salt of manganese, iron, zinc, antimony and tin, and add-on manganese salt accounts for the 0.5-2% of substratum quality percentage composition; Molysite accounts for the 2-5% of substratum quality percentage composition; Zinc salt accounts for the 1-2% of substratum quality percentage composition; Antimonic salt accounts for the 0.2-1% of substratum quality percentage composition; Pink salt accounts for the 0.3-1 of substratum quality percentage composition, get each and enlarge 20 kilograms of domestication cultivation and fermentation things (comprise domestication after bacterial classification and enzyme liquid), add 1 kilogram of manganous sulfate, 3 kilograms in ferrous sulfate, 1 kilogram in zinc sulfate, 0.2 kilogram of 0.3 kilogram of antimony trisulfate and tin protochloride and 80 kilograms of wooden chaffs, install in the hollow ceramic ball (appearance has many apertures), be placed in the tank body, yellow seriflux enters lays bioenzyme activity cat ceramic ball, starch flow is behind bioenzyme activity promotor carrier, fully contact with promotor with active biological enzyme, the complete decompose pectin of active biological enzyme after 30-60 minute, part is decomposed hemicellulose and Mierocrystalline cellulose, and outlet can improve 5% to 15%.
Claims (4)
1, a kind of method that improves alcohol output, be that carrier with the bioenzyme activity catalyzer is placed in the container, give the starch troubled liquor enter before the fermentor tank this container of flowing through, and stopped 30-60 minute, it is fully contacted, described bioenzyme activity catalyzer is polygalacturonase, cellulase and trace element, and described carrier adopts porous class material;
Wherein polygalacturonase (pectinex) accounts for the 8-15% of the quality percentage composition of whole active catalyst, and Mierocrystalline cellulose (Cellulose) quality percentage composition is 10-20%, and all the other are carriers;
Lus), Penicillium (Penicillium), Sporotrichum Sporotrichum), Sclerotinia (Sclerotinia) or black-koji mould (Aspergilus niger, AN);
Described cellulase obtains with strain fermentation, comprise Trichodermareesei (Trichoderma reesei Rut C-30), viride (Trichoderma virideAS3.3032), koning trichoderma (Trichoderma Koningii), whiterot fungi (Panusconchatu.), aspergillus (Aspergillus sp.), crassa (Neurospora sp.), head mold (Rhizopus), Penicillium (Penicillium) or black-koji mould (Aspergilus niger, AN);
Described trace element is the water-soluble inorganic salt of manganese, iron, zinc, antimony and tin, can select vitriol, nitrate or chlorate for use, and in these water-soluble inorganic salts, manganese salt accounts for the 0.5-2% of active catalyst quality percentage composition; Molysite accounts for the 2-5% of active catalyst quality percentage composition; Zinc salt accounts for the 1-2% of active catalyst quality percentage composition; Antimonic salt accounts for the 0.2-1% of active catalyst quality percentage composition; Pink salt accounts for the 0.3-1 of active catalyst quality percentage composition.
2, the method for raising alcohol output according to claim 1 is characterized in that: porous class material support adopts the sugarcane blob of slag of compacting, multilayer net or pottery, baton round or the plastic wire that sisal fibers is made into, pottery, the surperficial aperture that has of baton round.
3, the method of raising alcohol output according to claim 1, it is characterized in that: the polygalacturonase that is adopted, cellulase generation bacterium need add bacterial classification respectively substratum and carry out enlarged culturing 1-2 days, substratum adopts starch+peptone+sucrose or dextrose culture-medium commonly used in the document, every liter of substratum starch accounts for the 20-30 gram, peptone accounts for the 20-30 gram, sucrose or glucose 20-30 gram, sodium-chlor 5-10 gram, adding distilled water makes it reach 1 liter, also will adopt after the cultivation according to the yellow seriflux of fermentation concentration and tame 1 day again, bacterial classification and the enzyme liquid with domestication is filled into carrier together and is placed in the tank body more then.
4, the equipment of the method for raising alcohol output according to claim 1 employing, it is characterized in that: this equipment is the container that there are import and export upper and lower, inner porous class material of filling one deck or several layers mixed biologic enzymic activity catalyzer, this equipment is installed in the front of fermentor tank.
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Cited By (4)
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CN102643870A (en) * | 2012-05-14 | 2012-08-22 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
CN103436586A (en) * | 2013-07-18 | 2013-12-11 | 济南开发区星火科学技术研究院 | Process for producing alcohol by utilizing microorganisms to ferment biomass |
US20140352203A1 (en) * | 2013-05-28 | 2014-12-04 | Gui-zhong Song | Novel Environmentally-Friendly High-Energy Alcohol-based Industrial Fuel and Preparation Method Thereof |
US20160340599A1 (en) * | 2014-05-10 | 2016-11-24 | Gui-zhong Song | Novel Environmentally-Friendly High-Energy Alcohol-based Industrial Fuel and Preparation Method Thereof |
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CN103014074B (en) * | 2013-01-05 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Production method of high-concentration ethanol with sweet potatoes serving as raw materials |
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CN1173041C (en) * | 2003-05-21 | 2004-10-27 | 云南大学 | Production of alcohol by fermenting by yeast tolerant to high concentrated sugar and alcohol |
CN100342022C (en) * | 2005-12-20 | 2007-10-10 | 云南大学 | Method for improving alcohol yield fermented from starch material |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102643870A (en) * | 2012-05-14 | 2012-08-22 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
CN102643870B (en) * | 2012-05-14 | 2014-04-30 | 中国科学院过程工程研究所 | Method and device for preparing acetonebutanol by utilizing adsorption carrier fermentation |
US20140352203A1 (en) * | 2013-05-28 | 2014-12-04 | Gui-zhong Song | Novel Environmentally-Friendly High-Energy Alcohol-based Industrial Fuel and Preparation Method Thereof |
CN103436586A (en) * | 2013-07-18 | 2013-12-11 | 济南开发区星火科学技术研究院 | Process for producing alcohol by utilizing microorganisms to ferment biomass |
CN103436586B (en) * | 2013-07-18 | 2015-08-26 | 济南开发区星火科学技术研究院 | One utilizes fermentable biomass to produce alcohol technique |
US20160340599A1 (en) * | 2014-05-10 | 2016-11-24 | Gui-zhong Song | Novel Environmentally-Friendly High-Energy Alcohol-based Industrial Fuel and Preparation Method Thereof |
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