CN1985950A - Slow released capsule of cefotaxime sodium and bletilla tuber glue for animal and birds - Google Patents

Abstract

The present invention belongs to the field of veterinary medicine technology, and is especially slow released veterinary medicine capsule prepared with cefotaxime and bletilla tuber as material. The preparation process includes the following steps: water extracting and alcohol precipitation to extract bletilla tuber glue; mixing cefotaxime and bletilla tuber in the ratio of 1 to 3 and 10 % concentration alcohol solution of PVP as adhesive; making pellet of 20 meshes and encapsulating. The slow released veterinary medicine capsule has long medicine retaining time in the disease focus, high local medicine concentration, excellent bioadhesion and obvious medicine slow releasing characteristic.

Description

The slow released capsule of cefotaxime sodium and bletilla tuber glue that a kind of poultry are used

Technical field

The invention belongs to the technical field of veterinary drug, particularly relating to cefotaxime sodium, the Pseudobulbus Bletillae (Rhizoma Bletillae) is feedstock production veterinary drug slow released capsule of cefotaxime sodium and bletilla tuber glue and preparation method thereof.

Background technology

Culture towards intensive and intensive direction and develop along with the animal husbandry of China, the continuous expansion of scale is cultured in animal husbandry, ensures that the quality safety of animal husbandry product has become the present major issue that we need solve.At present, the problem that China's animal product mainly exists has: the one, and the threat of dangerous disease; The 2nd, drug residue.Wherein the drug residue in the animal food has jeopardized people ' s health.Therefore, rationally using animal veterinary drug and health product to control eqpidemic disease and drug residue in the herding production process, is the major issue that needs solution at present.

Bioadhesive drug delivery system has and can be detained the long period on mucosa, directly with the advantages such as focus of drug release at bacterial infection, improves curative effect of medication when reducing drug dose, alleviates side effects of pharmaceutical drugs.Bioadhesive drug delivery system is that medicine adheres to mucous epithelium by means of some macromolecular material to biological mucosa generation special adhesion power, thereby prolong drug makes medicine bring into play therapeutical effect better in the stop and the release time of corresponding site.The biologic adhesion preparation technology can be made medicine multiple dosage forms such as powder, tablet, membrane, capsule, is applicable to the mucosa delivery of different parts, as oral cavity, digestive tract, nasal mucosa etc.At beginning of the eighties late 1970s, Nagai adopts the adhesiveness polymer as anti-inflammatory steroid class pharmaceutical carrier first, and the topical treatment aphtha obtains better therapeutic effect.Types such as it is synthetic, natural and semi-synthetic that bioadhesive material has, desirable bioadhesive material should meet avirulence, excellent biological compatibility, easily and medicament mixed, requirements such as not influencing medicine assay, have good Release Performance, be cheap and easy to get.The natural biological adhesion material has: gelatin, pectin, arabic gum, sodium alginate etc.Synthetic bioadhesive material major part is polyacrylic acid or cellulose derivative, as sodium carboxymethyl cellulose (CMC-Na), hypromellose (HPMC), hydroxypropyl cellulose (HPC), polyvinyl alcohol (PEG) etc.Nontoxic, the good biocompatibility of natural material, but adhesiveness and Release Performance are undesirable; The adhesiveness and the Release Performance of synthetic material are better, and the price height, safety is undesirable.Therefore, there be limited evidence currently of has bioadhesive material to reach requirement fully.

Summary of the invention

The objective of the invention is to cefotaxime sodium, the Pseudobulbus Bletillae (Rhizoma Bletillae) at the problems referred to above is feedstock production veterinary drug slow released capsule of cefotaxime sodium and bletilla tuber glue, and prolong drug reaches valid density in the time of Entogastric lingering at gastric mucosa layer or surface epithelial cell.

For reach purpose of the present invention we to select cefotaxime sodium, the Pseudobulbus Bletillae (Rhizoma Bletillae) be feedstock production bioadhesive slow release capsule.The Pseudobulbus Bletillae (Rhizoma Bletillae) is the dry tuber of the orchid Pseudobulbus Bletillae (Rhizoma Bletillae), bitter in the mouth, sweet, puckery, and cold nature is traditional hemorrhage, has the effect of hemostasis convergence, detumescence and promoting granulation.Modern study shows, the Pseudobulbus Bletillae (Rhizoma Bletillae) has pharmacological actions widely such as antiulcer, anti-alimentary tract are hemorrhage, prevention intestinal adhesion, artificial embolism, antitumor.

A kind of phlegmatic temperament that Pseudobulbus Bletillae (Rhizoma Bletillae) glue system obtains through water extract-alcohol precipitation from the Pseudobulbus Bletillae (Rhizoma Bletillae), be the powder of white or yellowish-brown, tasteless, odorless, water soluble forms viscosity solution, be insoluble to ethanol, chemistry constitutes genus mucopolysaccharide macromolecular compound, is polymerized by 4 molecule mannose and 1 molecule glucose, and it is 183 that gel filtration records mean molecule quantity, 000, it is 2,881,000 that viscosimetry is measured viscosity-average molecular weight.Pseudobulbus Bletillae (Rhizoma Bletillae) glue is as the unique characteristics in aspects such as medicinal substrate and pharmaceutical carrier, for example, play the effect of suspending in preparation suspension type lotion, liniment, Pseudobulbus Bletillae (Rhizoma Bletillae) glue also day by day increases as the substrate of emulsifying agent, ultrasonic coupling agent, gel, facial cream, facial film with as the application of aspects such as the additive of the binding agent of tablet, eye drop and liniment.Pseudobulbus Bletillae (Rhizoma Bletillae) glue is as pharmaceutical carrier, can prolong the holdup time of principal agent, have characteristics such as functional slow-release, local anelasticity, self degradability, nonirritant, have no side effect, and can reduce the toxicity of contained medicine, and cheap, aboundresources, exploitation is worth having preferably aspect preparation targeting drug delivery system or slow release, the controlled release preparation.

The present invention utilizes the therapeutical effect of Pseudobulbus Bletillae (Rhizoma Bletillae) glue, and the characteristics that have functional slow-release, local adhesive retention as adjuvant, the veterinary drug slow released capsule of cefotaxime sodium and bletilla tuber glue is provided, it is characterized in that with Pseudobulbus Bletillae (Rhizoma Bletillae) glue be adhesion material, cefotaxime sodium is a model drug, 10%PVP (polyvinylpyrrolidone) alcoholic solution is made binding agent, prepares slow released capsule of cefotaxime sodium and bletilla tuber glue for animals.

Described veterinary drug slow released capsule of cefotaxime sodium and bletilla tuber glue, it is characterized in that, Pseudobulbus Bletillae (Rhizoma Bletillae) rubber powder is broken, cross 100 mesh sieves, add cefotaxime sodium, cefotaxime sodium is 1: 3 with Pseudobulbus Bletillae (Rhizoma Bletillae) glue ratio, abundant mixing, and 10%PVP (polyvinylpyrrolidone) alcoholic solution is made binding agent, make 20 order granules, dry about 60 ℃, granulate incapsulates.

Described veterinary drug slow released capsule of cefotaxime sodium and bletilla tuber glue, its feature also are, the preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) glue: get Pseudobulbus Bletillae (Rhizoma Bletillae) powder and be broken into coarse grain, add as 6-8 times of distilled water of medical material amount, flood half an hour with 60 ℃ of insulations, stir at any time, with four layers of filtered through gauze; The medicinal residues adding distil water repeats above operation 4 times, merging filtrate again, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, separate out a large amount of white flocculent deposits, leave standstill a night, filter next day, reclaims ethanol, the collecting precipitation thing with 95% washing with alcohol 3 times, volatilizes ethanol, oven dry about 80 ℃, weigh, pulverize, Pseudobulbus Bletillae (Rhizoma Bletillae) glue extraction ratio can reach 22%.

The invention effect: Pseudobulbus Bletillae (Rhizoma Bletillae) glue is the mucopolysaccharide macromolecular compound, is polymerized by 4 molecule mannose and 1 molecule glucose, can be used as pharmaceutical carrier, the prolong drug holdup time, improves medication effect.Have characteristics such as self degradability, nonirritant, have no side effect, cheap and easy to get, aboundresources, the exploitation that has as pharmaceutical carrier is worth;

Slow released capsule of cefotaxime sodium and bletilla tuber glue for animals is carried out interior evaluating: adopt left drug amount in the determined by ultraviolet spectrophotometry rat stomach tissue.Adopt high-efficient liquid phase chromatogram technique analysis blood drug level.Experimental result shows, take capsule 's content after 2 hours in the rat stomach tissue medicine retention rate be 43.7%, significantly, significantly improved the local concentration of medicine at gastric greater than the retention rate 15.2% of cefotaxime sodium.The pharmacokinetics experiment shows that this capsule particle absorbs with first-rate, and the blood drug level peaking time is 4～5 hours.Left drug scale at the animal gastric after the administration is bright, but slow released capsule of cefotaxime sodium and bletilla tuber glue content prolong drug improves the local concentration of medicine at gastric tissue in the holdup time of gastric, has good bioadhesive.

The slow released capsule of cefotaxime sodium and bletilla tuber glue content has the drug release characteristic of slow releasing preparation, thereby prolonged delay and the release time of medicine at corresponding site, make medicine bring into play therapeutical effect better, also improved whole bioavailability, and obviously improved curative effect of medication and suffered from the compliance of raiseeing.

The specific embodiment

Embodiment 1: the preparation slow released capsule of cefotaxime sodium and bletilla tuber glue

The preparation of a, Pseudobulbus Bletillae (Rhizoma Bletillae) glue: Pseudobulbus Bletillae (Rhizoma Bletillae) 5kg, pulverize adding distil water 35L, 60 ℃ of insulation dippings of heating in water bath, stir, extracting solution is through four layers of filtered through gauze, and medicinal residues are adding distil water again, repeated impregnations, merge filtrate several times, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, separate out a large amount of white flocculent deposits, leave standstill a night, filter next day, reclaims ethanol, the collecting precipitation thing, with washing with alcohol 3 times, volatilize ethanol, oven dry about 80 ℃, weigh, pulverize, can get Pseudobulbus Bletillae (Rhizoma Bletillae) glue 1.1kg; Pseudobulbus Bletillae (Rhizoma Bletillae) glue: character is yellow dry powder, and tasteless odorless is insoluble to ethanol, and is water-soluble, is stiff colloidal.Alpha-Naphthol concentrated sulphuric acid and the experiment of alkaline cupric tartrate test solution are positive reaction, and this product contains alcohol-insoluble substances and is no less than 80%.

B, preparation slow released capsule of cefotaxime sodium and bletilla tuber glue: get Pseudobulbus Bletillae (Rhizoma Bletillae) glue 0.9kg, cefotaxime sodium 0.3kg, Pseudobulbus Bletillae (Rhizoma Bletillae) rubber powder is broken, add cefotaxime sodium after crossing 100 mesh sieves, fully mixing is got 10%PVP (polyvinylpyrrolidone) alcoholic solution and is made binding agent, makes 20 order granules, dry about 60 ℃, granulate incapsulates, and promptly gets slow released capsule of cefotaxime sodium and bletilla tuber glue.

Embodiment 2: the slow released capsule of cefotaxime sodium and bletilla tuber glue for preparing is used for the treatment of infection such as respiratory tract that poultry cause because of gram negative bacteria, urinary tract, biliary tract, intestinal.Administering mode is spice or drinking-water administration, poultry 0.05-0.1g/kg body weight, and domestic animal 0.02-0.03g/kg body weight, 2 times on the one, 3-5 days is a course of treatment.

Claims (3)

1, veterinary drug slow released capsule of cefoperazone and bletilla tuber glue is characterized in that with Pseudobulbus Bletillae (Rhizoma Bletillae) glue be adhesion material, and cefoperazone is a model drug, and alcoholic solution is made binding agent, the preparation slow released capsule of cefoperazone and bletilla tuber glue.

2, veterinary drug slow released capsule of cefoperazone and bletilla tuber glue according to claim 1, Pseudobulbus Bletillae (Rhizoma Bletillae) rubber powder is broken, cross 100 mesh sieves, add cefoperazone, cefoperazone is 1: 3 with Pseudobulbus Bletillae (Rhizoma Bletillae) glue ratio, abundant mixing, 10%PVP (polyvinylpyrrolidone) alcoholic solution is made binding agent, makes 20 order granules, and is dry about 60 ℃, granulate incapsulates.

3, veterinary drug slow released capsule of cefoperazone and bletilla tuber glue according to claim 1, its feature also is, the preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) glue: get Pseudobulbus Bletillae (Rhizoma Bletillae) powder and be broken into coarse grain, add as 6-8 times of distilled water of medical material amount, flood half an hour with 60 ℃ of insulations, stir at any time, with four layers of filtered through gauze; The medicinal residues adding distil water repeats above operation 4 times, merging filtrate again, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, separate out a large amount of white flocculent deposits, leave standstill a night, filter next day, reclaims ethanol, the collecting precipitation thing with 95% washing with alcohol 3 times, volatilizes ethanol, oven dry about 80 ℃, weigh, pulverize, Pseudobulbus Bletillae (Rhizoma Bletillae) glue extraction ratio can reach 22%.