CN1974755A - Yeast strain for producing erythritol - Google Patents
Yeast strain for producing erythritol Download PDFInfo
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- CN1974755A CN1974755A CN 200610163644 CN200610163644A CN1974755A CN 1974755 A CN1974755 A CN 1974755A CN 200610163644 CN200610163644 CN 200610163644 CN 200610163644 A CN200610163644 A CN 200610163644A CN 1974755 A CN1974755 A CN 1974755A
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Abstract
The present invention is yeast strain capable of converting glucose into erythritol. The yeast strain features its deficiency in motile spore, septate mycelium, asexual propagation, deficiency in kidney-shaped cell, formation of conidium in optional dwarf denticle, deficiency in ballistoconidium, capacity of growing in 25 deg.c-36 deg.c, etc.
Description
The application is that July 21, application number in 2000 are 00121352.0, denomination of invention is divided an application for " producing the yeast strain of erythritol " for the applying date.
Erythritol belongs to glycitols, and its sugariness is 60% to 80% of a sucrose, but its calorific value only is about 1/10 of sucrose, and can not cause carious tooth.Different with a lot of sugar alcohols is that it can not cause diarrhoea.In addition, erythritol has fabulous processing characteristics: it is heat-staple; , therefore can not cause organism to become brown with the amino group reaction.
Erythritol is found in lichens, macrofiber leaf, fungi plant, leavened food (as grape wine and soy sauce) and the microorganism.The microorganism that can produce erythritol comprises the yeast strain of pichia genus, mycocandida, torulopsis, Trigonopsis, Moniliella, Aureobasidium and Trichosporon.
The present invention relates in simple culture media, glucose to be changed into the new yeast strain of erythritol.
Yeast strain of the present invention is characterised in that shortage energy zoospore or zoospore (that is the spore that, has flagellum); Has septate mycelium (that is the mycelium that, has bulkhead); Vegetative propagation (that is, not relating to kasogamy and maiotic breeding); Lack kidney shape cell; Conidium is chosen wantonly on short little tooth (that is, little dentation projection) rather than long handle and forms; Lack and throw conidium (that is the conidium that discharges by force); Non-on wide base-one pole sprout (that is, one pole projection do not produce new cell breeding); Tectorum blastoconidium chain (that is, being characterised in that before initiating cell was delimited by partition, it is big that the original cell of identifiable conidium significantly becomes); Dark-brown, the heavy-walled chlamydospore (that is, and by the contraction of protoplastis, each endogenous ground of asexual 1-locellus spore and be derived from the part of the cell that is pre-existing in by oneself); Fermentation capacity (that is the ability of at least a carbon source of half anaerobism ground fermentation) is arranged; Can assimilate sucrose, glycerine and maltose; Can not assimilate lactose; Unfermentable semi-lactosi; Can be at 25 ℃, 30 ℃, 35 ℃ and 36 ℃ of growths.Randomly, the feature of yeast strain of the present invention also is the sucrose that can ferment, glucose (that is D-glucose) or maltose; Or can maybe can not assimilate semi-lactosi.
Can measure bacterial strain according to the method for describing among the hereinafter actual embodiment and can ferment or assimilate specific carbon source, can in the substratum that does not contain VITAMIN, grow, can under specific temperature, grow.
Also desire in the scope of the present invention to comprise to have and be characterised in that above-mentioned morphology proterties and following table 1,2,3,4, the yeast strain of physiology proterties described in 5 or 6.The example includes but not limited to be preserved on January 27th, 2000 American type culture collection (10801 UniversityBlvd., Manassas, VA 20110-2209,6 bacterial strains USA).The preserving number of preservation strain is PTA-1227, PTA-1228, PTA-1229, PTA-1230, PTA-1231 and PTA-1232.Derived from the mutant of preservation strain also within the scope of the invention.
Yeast strain of the present invention and Moniliella acetobuten are the most approaching.In fact, their above-mentioned morphology proterties is consistent with M.acetobuten.On the other hand, the difference of their physiologic character and M.acetobuten is very little.For example, different with M.acetobuten is that bacterial strain of the present invention can not assimilate lactose.In addition, they can change glucose into erythritol with the speed more much higher than M.acetobuten in simple culture media (as only containing glucose and yeast extract).
Apparent further feature of the present invention or advantage from following detailed description (comprising embodiment) and appended claims.
Yeast strain of the present invention is separable from natural origin, as has the sample of high sugared content, as honey, and preserved fruit and pollen.Change glucose into the ability of erythritol and different morphology and each bacterial strains of physiology macroscopical identification thereof according to it.Yeast strain of the present invention can be changed 1g glucose into 0.3g erythritol (being transformation efficiency 〉=30%) at least.30 ℃, in the 50ml flask, (initial cell density was 1 * 10 to rotational oscillation cultivation bacterial strain in 6 days in the 10ml meat soup that contains 30% glucose and 1% yeast extract
5Individual cell/ml) is measured transformation efficiency.20 ℃, the morphology proterties is measured in growth after 10 days in 4% malt extract/0.5% yeast extract agar.Referring to yeast, means of taxonomic research, volumes such as Kurtzman, the 4th edition, p785, Elsevier, Amsterdam (1998).On the other hand, measure the physiology proterties by the method described in the embodiment hereinafter.
Other yeast strain of the present invention can be the varient derived from the bacterial strain that is located away from natural origin.For example, these bacterial strains can be that N-methyl-N '-nitrosoguanidine is handled by the UV irradiation, and ethyl methane sulfonate is handled, and nitrous acid is handled, the mutant that bifurcation pyridine processing etc. obtains.They also can be to utilize cytogamy or the recombinant DNA technology recombinant bacterial strain through genetic engineering modified generation.
Only in order to illustrate rather than limit the specific embodiment of its disclosure, those skilled in the art can obtain and farthest utilize yeast strain of the present invention according to following.All publications that this paper mentions are all listed this paper in as a reference.
Embodiment
370 sample collectings are from honey, and thick pollen is through pollen, preserved fruit, fresh fruit, wastewater from sugar plant and the molasses of processing.Then in the substratum that contains 40% glucose and 1% yeast extract with the sample cultivation of gathering 3 to 4 days.Culture is laid on the agar plate that contains 20% glucose and 1% yeast extract, and insulation is 3 to 4 days in 30 ℃ of incubators.Select different bacterial strains according to the outward appearance of bacterium colony, the inoculation that will choose is in the meat soup that contains 30% glucose and 1% yeast extract, and insulation is 4 to 5 days in 30 ℃ of incubators.Measure the amount of each supernatant liquor mesoerythrit by HPLC and TLC and produce the ability of erythritol to measure isolated strains.
Use Hewlett Packard H4033A analyser, temperature is set to 75 ℃ on the Ion-300 chromatography column,, carries out HPLC with 0.4ml/ minute flow velocity and analyzes as moving phase with 0.1N sulfuric acid.Carrying out TLC according to the method for Neissner etc. (Neissner etc., 1980, the manufacturing of erythritol fatty acid ester, analysis and DC-separate, FETTE ' SEIFEN ' ANSTRICHMITTEL.82:10-16) analyzes.With 4% boric acid flushing Kieselgel 60F254 (Merck) afterwards, also gel should be placed 105 ℃ incubator to heat before the use 20 minutes.The diffusion solvent is an ethyl ketone: acetone: water (volume ratio is 100: 10: 10), chromogenic agent is the KMnO that is dissolved in the vitriol oil
4
Be further purified the erythritol in supernatant liquor by extraction, carry out drying under reduced pressure then through HPLC or TLC purifying.Method (Shindou etc. according to Shindou etc.; 1989; identify the erythritol in the multiple pulp and carry out quantitative assay that by HPLC and GC-MS the agricultural food product The Chemicals 37:1474-1476) carries out acetylize with product and erythritol standard through being further purified.Whether detect the gained sample by GC-MS identical with standard model to measure through the product of purifying once more.
Isolate 630 strain bacterium from 370 samples altogether, wherein 22 strains demonstrate the ability of producing erythritol.From through the honey of processing, through the pollen of processing and molasses, obtain 161 strain isolates, therefrom to select 6 strain erythritols and produce bacterium, its erythritol transformation efficiency is 0.5 to 1.5%.Low-conversion may be due to the microorganism of production erythritol disappears, because before collected specimens, has been heated and drying.Find separating in 26 strain bacterium of 49 honey samples (great majority are without processing) has 3 strains, promptly 440,441 and 442 can high conversion (＞30%) by the glucose production erythritol.None demonstrates the ability of producing erythritol in 66 strain bacterium of wastewater from sugar plant and mud sample in separation.From thick pollen and preserved fruit sample, isolate 3 good erythritols and produce bacterial strain (transformation efficiency＞30%), i.e. 166-2,262-1 and 278-3.
In order to study the influence that glucose concn is produced erythritol, in 30 ℃, respectively in the substratum that contains 1% yeast extract and 20%, 30% and 40% glucose with the rotating speed shake-flask culture bacterial strain 166-2 of 150rpm, 262-1 and 278-3 reach 1 to 6 day (50ml flask, 10ml substratum; Initial cell density is 1 * 10
5Individual cell/ml).Measure the amount of the erythritol that is produced then.The result shows that the 3 strain bacterium of cultivating 6 days (measuring the standard method of yeast strain transformation efficiency of the present invention) all demonstrate the transformation efficiency above 30% in 30% dextrose culture-medium.In addition, cultivate after 6 days in the substratum that contains 40% glucose, the transformation efficiency of all 3 strain bacterium all is about 30%.As for the consumption of glucose, when cultivating in the substratum that contains 30% glucose, bacterial strain 166-2 consumed all glucose at the 5th day, and bacterial strain 262-1 and 278-3 have consumed all glucose at the 6th day.
In 30% dextrose culture-medium, add respectively 0.5,1.0 and 1.5M KCl or NaCl carried out studies show that: the increase of ionic osmotic pressure reduces the transformation efficiency of all 3 strain bacterium.Studies show that of pH distribution plan: at pH4.0-7.0, the transformation efficiency of the 3 strain bacterium that grow in 30% dextrose culture-medium is roughly the same.Transformation efficiency reduces during pH8.In 30% substratum, cultivate bacterial strain 166-2,262-1 and 278-3 respectively at 25 ℃, 30 ℃ and 35 ℃.All 3 strain bacterium demonstrate the highest transformation efficiency in the time of 30 ℃, demonstrate minimum transformation efficiency in the time of 25 ℃.
Also studied the influence that different substratum are produced erythritol.When 30% glucose was substituted by 30% maltose, all 3 strain bacterium did not produce erythritol.Substitute 30% glucose or transformation efficiency is significantly reduced with 30% Star Dri 5 with 6% corn diffusion juice or alternative 1% yeast extract of 6% bean powder.In addition, also studied of the influence of the yeast extract of different concns in the substratum (0.5%, 0.75% and 1.0%) to erythritol production.The result shows that transformation efficiency increases pari passu with the increase of yeast extract level.In addition, add the several mineral materials of different concns, i.e. MgSO
47H
2O (0.02% to 0.1%), K
2HPO
4(0.001% to 0.02%), CaCl
22H
2O (0.1% to 0.4%) and CaCO
3(0.1% to 1%) can the remarkably influenced transformation efficiency.
Except in flask, the cultivation,, in containing 5 liters of fermentor tanks of 2 liter of 30% glucose/1% yeast extract substratum, respectively bacterial strain 166-2,262-1 and 278-3 being cultivated 6 to 7 days (rotating speeds: 150rpm also in 30 ℃ by above-mentioned; Ventilation: 1vvm or volume/minute/culture volume; Initial cell density is 1 * 10
5Individual cell/ml).Bacterial strain 166-2 and 262-1 had roughly finished the conversion of glucose to erythritol when finishing in the 5th day.Bacterial strain 278-3 changed glucose into erythritol with slower speed, remained unfulfilled conversion until the 7th day.
In the time of the 7th day, the supernatant liquor of centrifugal each culture of collection is used activated carbon decolorizing, makes it then by ion exchange resin (IRA-410: IRA-120B=2: 1) to remove impurity.By evaporation and recrystallization concentrated after, obtain the erythritol of white limpid lenticular.Further confirm the structure of gained erythritol crystal by 1H and 13CNMR.
Found that when in 30 ℃ in the 50ml flask, (initial cell density is 1 * 10 in the time of 6 days to contain in the substratum of 30% glucose/1% yeast extract each shake-flask culture of rotating speed with 150rpm at 10ml
5Individual cell/ml) finds that every liter of 166-2,262-1,278-3,440,441 and 442 bacterial strains can produce 98.7,104.1,117,99,97.8 and the 102.6g erythritol respectively.
Measure the physiology proterties of 166-2,262-1,278-3,440,441 and 442 bacterial strains according to " yeast, means of taxonomic research " (volume such as Kreger-van Rij, the 3rd edition, p76-101, Elsevier, Amsterdam (1994)) method that provides.
Specifically, detect the fermentation of all sugar in the Durham pipe in 2% (w/v) solution, inoculum after the inoculation, in 25 ℃ of incubation cells in the fermentation minimum medium, is seen p78-79 from 48 hours cultures of malt extract agar.
Carry out aerobic utilization (assimilation) test of carbon compound according to the method described in " 1. liquid nutrient medium assimilation test " joint among the p81-83.More particularly, prepare cell suspending liquid, after the inoculation, in 25 ℃ of incubation cells with aseptic tap water.By with 6.7g Bacto yeast nitrogen base with an amount of carbon compound of glucose equivalent (promptly with 5g glucose carbonaceous amount identical) be dissolved in 100ml and go to prepare 10 times of spissated substratum in the water of mineral materialization.For simplicity, all positive reactions (being 1+, 2+ and 3+) all are represented as "+".
Carry out aerobic utilization (assimilation) test of nitrogenous compound according to the method described in " the 1. assimilation in the liquid nutrient medium " among p85-86 joint.For simplicity, all positive reactions (being 1+, 2+ and 3+) all are represented as "+".
Carry out growth test in the substratum that does not contain VITAMIN according to the method described in " the 3. growth in the substratum that does not contain VITAMIN, vitamin requirement " among the p86-87 one joint.For simplicity, all positive reactions (being 1+, 2+ and 3+) all are represented as "+".
According to " the 5. growth under 37 ℃ and other temperature among the p88-89; The maximum temperature of growth " method described in the joint carries out growth test under various temperature.What use is solid medium.
All test-results are shown in following table 1 to 6:
The physiologic character of table 1 bacterial strain 166-2
| Half anaerobically fermenting D-glucose maltose lactose | + + - | D-semi-lactosi sucrose | - + |
| Aerobic utilization and growth: D-Glucose sorbose D-ribose Arabinose D-rhamnose maltose methyl α-D-glucoside salicin melibiose gossypose synanthrin glycerine ribitol Arabinose alcohol D-mannital inositol 2-ketone-gluconate galacturonic hydrochlorate succinate methyl alcohol propane quinate Galactonate D-galactolipin aminoglucose wood sugar D-R sucrose | + - + W - + - - - - - + - - + - - - + - - - + - - W + | α; α-trehalose cellobiose ursin lactose melezitose starch antierythrite xylitol D-Glucitol galactitol D-gluconic acid-1,5-lactone D-gluconate DL-LACTIC ACID saline citrate Enthanol butane saccharate nitrate ethamine cadaverine kreatinin imidazoles nitrite lysine creatine aminoglucose | - + + - - - + - - - + - - - + - - + - + - - + + - - |
| Growth temperature: 25 ℃ 35 ℃ | + + | 30℃ 36℃ | + + |
The physiologic character of table 2 bacterial strain 262-1
| Half anaerobically fermenting D-glucose maltose lactose | + + - | D-semi-lactosi sucrose | - + |
| Aerobic utilization and growth: D-Glucose sorbose D-ribose Arabinose D-rhamnose maltose methyl α-D-glucoside salicin melibiose gossypose synanthrin glycerine ribitol Arabinose alcohol D-mannital inositol 2-ketone-gluconate galacturonic hydrochlorate succinate methyl alcohol propane quinate Galactonate D-galactolipin aminoglucose wood sugar D-R sucrose | + - + W - + - - - - - + - - + - - - + - - - - - - - - + | α; α-trehalose cellobiose ursin lactose melezitose starch antierythrite xylitol D-Glucitol galactitol D-gluconic acid-1,5-lactone D-gluconate DL-LACTIC ACID saline citrate Enthanol butane saccharate nitrate ethamine cadaverine kreatinin imidazoles nitrite lysine creatine aminoglucose | - + + - - - + - - - + - - - + - - + W + - - + + - - |
| Growth temperature: 25 ℃ 35 ℃ | + + | 30℃ 36℃ | + + |
The physiologic character of table 3 bacterial strain 278-3
| Half anaerobically fermenting D-glucose maltose lactose | + + - | D-semi-lactosi sucrose | - + |
| Aerobic utilization and growth: D-Glucose sorbose D-ribose Arabinose D-rhamnose maltose methyl α-D-glucoside salicin melibiose gossypose synanthrin glycerine ribitol Arabinose alcohol D-mannital inositol 2-ketone-gluconate galacturonic hydrochlorate succinate methyl alcohol propane quinate Galactonate D-galactolipin aminoglucose wood sugar D-R sucrose | + - - - - + - - - - - + - - + - - - + - - - - - - - - + | α; α-trehalose cellobiose ursin lactose melezitose starch antierythrite xylitol D-Glucitol galactitol D-gluconic acid-1,5-lactone D-gluconate DL-LACTIC ACID saline citrate Enthanol butane saccharate nitrate ethamine cadaverine kreatinin imidazoles nitrite lysine creatine aminoglucose | - + + - - - + - - - + - - - + - - + - + - - + + W - |
| Growth temperature: 25 ℃ 35 ℃ | + + | 30℃ 36℃ | + + |
The physiologic character of table 4 bacterial strain 440
| Half anaerobically fermenting D-glucose maltose lactose | + + - | D-semi-lactosi sucrose | - + |
| Aerobic utilization and growth: D-Glucose D-galactolipin sorbose GLUCOSAMINE D-ribose Arabinose D-R L-rhamnose sucrose maltogenic alpha, α-trehalose methyl α-D-glucoside cellobiose salicin ursin melibiose lactose gossypose melezitose synanthrin glycerinum amyli antierythrite ribitol xylitol Arabinose alcohol D-Glucitol | + + + - + - - - + + - - + - + - - - - - - + + - - - - | The growth that D-mannital galactitol inositol D-gluconic acid-1,5-lactone D-gluconate D-glucuronate D-galacturonic acid saline citrate DL-LACTIC ACID salt succinate methyl alcohol 2-ketone-gluconate D-wood sugar ethanol propane saccharate butane quinate Galactonate Nitrate, Nitrite ethamine 1B cadaverine do not contain vitamin contains 0.01% (w/v) cycloheximide and contains 0.1% (w/v) cycloheximide | + - - + - - - - - + - - - + - - - - - + + - + + + - - |
| Growth temperature: 25 ℃ 35 ℃ | + + | 30℃ 36℃ | + + |
The physiologic character of table 5 bacterial strain 441
| Half anaerobically fermenting D-glucose maltose lactose | - - - | D-semi-lactosi sucrose | - - |
| Aerobic utilization and growth: D-Glucose D-galactolipin sorbose GLUCOSAMINE D-ribose Arabinose D-R L-rhamnose sucrose maltogenic alpha, α-trehalose methyl α-D-glucoside cellobiose salicin ursin melibiose lactose gossypose melezitose synanthrin glycerinum amyli antierythrite ribitol xylitol Arabinose alcohol D-Glucitol | + - + - + - - - + + - - + - + - - - - - - + + - - - - | The growth that D-mannital galactitol inositol D-gluconic acid-1,5-lactone D-gluconate D-glucuronate D-galacturonic acid saline citrate DL-LACTIC ACID salt succinate methyl alcohol 2-ketone-gluconate D-wood sugar ethanol propane saccharate butane quinate Galactonate Nitrate, Nitrite ethamine 1B cadaverine do not contain vitamin contains 0.01% (w/v) cycloheximide and contains 0.1% (w/v) cycloheximide | + - - + W - - - - + - - - + - - - - - + + - + + + - - |
| Growth temperature: 25 ℃ 35 ℃ | + + | 30℃ 36℃ | + + |
The physiologic character of table 6 bacterial strain 442
| Half anaerobically fermenting D-glucose maltose lactose | + + - | D-semi-lactosi sucrose | - + |
| Aerobic utilization and growth: D-Glucose D-galactolipin sorbose GLUCOSAMINE D-ribose Arabinose D-R L-rhamnose sucrose maltogenic alpha, α-trehalose methyl α-D-glucoside cellobiose salicin ursin melibiose lactose gossypose melezitose synanthrin glycerinum amyli antierythrite ribitol xylitol Arabinose alcohol D-Glucitol | + - + - + - - - + + - - + - + - - + - - - + + - - - - | The growth that D-mannital galactitol inositol D-gluconic acid-1,5-lactone D-gluconate D-glucuronate D-galacturonic acid saline citrate DL-LACTIC ACID salt succinate methyl alcohol 2-ketone-gluconate D-wood sugar ethanol propane saccharate butane quinate Galactonate Nitrate, Nitrite ethamine 1B cadaverine do not contain vitamin contains 0.01% (w/v) cycloheximide and contains 0.1% (w/v) cycloheximide | + - - + - - - W - + - - - + - - - - - + + - + + + - - |
| Growth temperature: 25 ℃ 35 ℃ | + + | 30℃ 36℃ | + + |
According to above description, those skilled in the art can easily determine essential features of the present invention, under the situation that does not deviate from the spirit and scope of the present invention, can carry out multiple change and modification so that it adapts to multiple use and condition to the present invention.For example, can be grown in the substratum that only contains 30% glucose and 1% yeast extract although all try yeast strain, those bacterial strains of only growing in being added with one or more nutraceutical substratum are also included within the scope of the present invention.Therefore, other embodiment is also included within claims.
Claims (6)
1. preserving number is the yeast strain of PTA-1227.
2. preserving number is the yeast strain of PTA-1228.
3. preserving number is the yeast strain of PTA-1229.
4. preserving number is the yeast strain of PTA-1230.
5. preserving number is the yeast strain of PTA-1231.
6. preserving number is the yeast strain of PTA-1232.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101636448A CN1974755B (en) | 2000-07-21 | 2000-07-21 | Yeast strain for producing erythritol |
| HK07111308.7A HK1105996A1 (en) | 2000-07-21 | 2002-06-21 | Erythritol-production yeast strains |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00121352 CN1335391A (en) | 2000-07-21 | 2000-07-21 | Saccharomycetes strain for producing erythritol |
| CN2006101636448A CN1974755B (en) | 2000-07-21 | 2000-07-21 | Yeast strain for producing erythritol |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00121352 Division CN1335391A (en) | 2000-07-21 | 2000-07-21 | Saccharomycetes strain for producing erythritol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1974755A true CN1974755A (en) | 2007-06-06 |
| CN1974755B CN1974755B (en) | 2010-06-16 |
Family
ID=4588747
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101636448A Expired - Lifetime CN1974755B (en) | 2000-07-21 | 2000-07-21 | Yeast strain for producing erythritol |
| CN 00121352 Pending CN1335391A (en) | 2000-07-21 | 2000-07-21 | Saccharomycetes strain for producing erythritol |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00121352 Pending CN1335391A (en) | 2000-07-21 | 2000-07-21 | Saccharomycetes strain for producing erythritol |
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| Country | Link |
|---|---|
| CN (2) | CN1974755B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199551A (en) * | 2010-12-17 | 2011-09-28 | 南京工业大学 | Yeast and method for producing erythritol by multi-stage fermentation of yeast |
| CN103224891A (en) * | 2013-05-21 | 2013-07-31 | 江苏省微生物研究所有限责任公司 | Torula.sp strain and method for producing erythritol from same through fermentation in shake flask and 5L fermentation tank |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101071115B (en) * | 2007-06-15 | 2013-07-03 | 淄博中食歌瑞生物技术有限公司 | Erythritol production method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4939091A (en) * | 1986-09-09 | 1990-07-03 | Director Of National Food Research Institute, Ministry Of Agriculture, Forestry And Fisheries | Novel auerobasidium sp. microorganisms, method for obtaining the same and method for preparing erythritol with the same |
| JPH0734749B2 (en) * | 1988-02-03 | 1995-04-19 | 日本碍子株式会社 | Method for producing erythritol |
| DE69720379T2 (en) * | 1996-12-02 | 2004-02-12 | Mitsubishi Chemical Corp. | Process for the preparation of erythritol |
-
2000
- 2000-07-21 CN CN2006101636448A patent/CN1974755B/en not_active Expired - Lifetime
- 2000-07-21 CN CN 00121352 patent/CN1335391A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199551A (en) * | 2010-12-17 | 2011-09-28 | 南京工业大学 | Yeast and method for producing erythritol by multi-stage fermentation of yeast |
| CN102199551B (en) * | 2010-12-17 | 2012-09-19 | 南京工业大学 | Yeast and method for producing erythritol by multi-stage fermentation of yeast |
| CN103224891A (en) * | 2013-05-21 | 2013-07-31 | 江苏省微生物研究所有限责任公司 | Torula.sp strain and method for producing erythritol from same through fermentation in shake flask and 5L fermentation tank |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1335391A (en) | 2002-02-13 |
| CN1974755B (en) | 2010-06-16 |
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