CN1974604A - Prepn process of branched cyclodextrin - Google Patents
Prepn process of branched cyclodextrin Download PDFInfo
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- CN1974604A CN1974604A CN 200610098196 CN200610098196A CN1974604A CN 1974604 A CN1974604 A CN 1974604A CN 200610098196 CN200610098196 CN 200610098196 CN 200610098196 A CN200610098196 A CN 200610098196A CN 1974604 A CN1974604 A CN 1974604A
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- cyclodextrin
- beta
- maltose
- branching
- pullulanase
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Abstract
The preparation process of branched cyclodextrin belongs to the field of cyclodextrin preparing technology. Beta-cyclodextrin and maltose as the materials are prepared into the branched cyclodextrin through the reverse synthetic effect of pullulan enzyme. The branched cyclodextrin product has high water solubility over 160 g/100 ml, may dissolve many oily matters to generate soluble inclusion capable of being preserved at 90 deg.c for 7 hr without loss, and has relatively high solubility in ethanol, methanol, acetone, glycol, etc. The branched cyclodextrin may be applied in food, medicine, cosmetics, additive, essence and other fields.
Description
Technical field
A kind of preparation method of branching cd, relating to a kind of is raw material with beta-cyclodextrin and maltose, by the reverse Synthesis of Pullulanase, prepares the method for branching cd, belongs to the cyclodextrin technical field.
Background technology
Cyclodextrine is an alpha-D-glucose, passes through the ring-type malto-oligosaccharide that α-1,4 glycosidic link is formed by connecting by six above glucose.The cyclodextrine product is according to the number of glucosyl residue, is called alpha-cylodextrin (α-CD), beta-cyclodextrin (β-CD), γ-Huan Hujing (γ-CD).Cyclodextrine has the character that is better than the straight chain dextrin because of its distinctive space structure.Its space structure is the cylindric of hollow, and the hole internal diameter is 6-10 , can be by having a lot of purposes with the compound formation inclusion compound.Can stablize multiple volatile matter; Sensitive materials such as light, heat, oxygen are shielded; Can change the physico-chemical property of original material,, in industries such as food, medicine, agricultural chemicals, fine chemistry industry, all have purposes very widely as solubleness, water absorbability, local flavor, color and luster etc.
But at present three kinds of cyclodextrines producing all have its deficiency: cyclohexaamylose hole internal diameter is little, about 5-6 , usually can only inclusion than the guest species of small molecules, range of application is little; The hole internal diameter of cyclooctaamylose is big, about 9-10 , and the more macromolecular guest species of energy inclusion, but the production cost height of cyclooctaamylose, its application is restricted; The hole internal diameter of cycloheptaamylose is moderate, about 7-8 , and the inclusion scope is wide, and production cost is low, be at present unique at the mass-produced cyclodextrin product of industrial energy, but the solubleness of cycloheptaamylose in water is low, has limited its range of application.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of branching cd, branching cd is one or several glucosyl residues of coupling and the cyclodextrin derivative that forms on the hydroxyl of the 6th carbon atom of certain glucosyl residue of cyclodextrine.Has the character that much is better than mother body cyclodextrin.
Technical scheme of the present invention: with beta-cyclodextrin and maltose is raw material, reverse Synthesis by Pullulanase, the preparation branching cd, the quality proportioning of raw material is in the reaction system: maltose 55%-59%, beta-cyclodextrin 11%-15%, amount of water 30%, the addition of Pullulanase is the 100-200U/g beta-cyclodextrin, processing step is: by proportioning beta-cyclodextrin is added the gauge water heating for dissolving, add the maltose dissolving then, solution is cooled to 65-70 ℃, adjusting the pH value is 4.0-4.5, add Pullulanase reaction 60 hours, be heated to 100 ℃, 15 minutes enzymes that go out, adopt the separation and purification of SephadexG25 gel column, 60 ℃ of vacuum-dryings are dried to moisture content less than 13%, obtain the branching cd product.
Beneficial effect of the present invention: branching cd is one or several glucosyl residues of coupling and the cyclodextrin derivative that forms on the hydroxyl of the 6th carbon atom of certain glucosyl residue of cyclodextrine.Has the character that much is better than mother body cyclodextrin.Solubleness is much higher than its corresponding cycloheptaamylose in water, can generate the solubility inclusion compound, and the inclusion compound of generation can keep not losing in 7 hours at 90 ℃, and the inclusion compound that mother body cyclodextrin forms can only remaining 45%.And it also has higher solubleness in ethanol, methyl alcohol, acetone, ethylene glycol etc.
The branching cd main component of the present invention's preparation is the malt-base cyclodextrin, and the solubleness in water can be dissolved a lot of oily matters greater than 160g/100mL.
Embodiment
Embodiment 1
The quality proportioning of raw material is in the reaction system: maltose 55%, beta-cyclodextrin 15%, amount of water 30%, the addition of Pullulanase is the 200U/g beta-cyclodextrin, processing step is: by proportioning beta-cyclodextrin is added the gauge water heating for dissolving, add the maltose dissolving then, solution is cooled to 70 ℃, and adjusting the pH value is 4.5, adds Pullulanase reaction 60h, be heated to 100 ℃, 15 minutes enzymes that go out adopt the separation and purification of SephadexG25 gel column, 60 ℃ of vacuum-dryings, being dried to moisture content is 12.56%, obtains the branching cd product.
Embodiment 2
The quality proportioning of raw material is in the reaction system: maltose 59%, beta-cyclodextrin 19%, amount of water 30%, the addition of Pullulanase is the 100U/g beta-cyclodextrin, processing step is: by proportioning beta-cyclodextrin is added the gauge water heating for dissolving, add the maltose dissolving then, solution is cooled to 65 ℃, and adjusting the pH value is 4.0, adds Pullulanase reaction 60h, be heated to 100 ℃, 15 minutes enzymes that go out adopt the separation and purification of SephadexG25 gel column, 60 ℃ of vacuum-dryings, being dried to moisture content is 12.12%, obtains the branching cd product.
Claims (1)
1, a kind of preparation method of branching cd, it is characterized in that with beta-cyclodextrin and maltose be raw material, reverse Synthesis by Pullulanase, the preparation branching cd, the quality proportioning of raw material is in the reaction system: maltose 55%-59%, beta-cyclodextrin 11%-15%, amount of water 30%, the addition of Pullulanase are the 100-200U/g beta-cyclodextrin, and processing step is: by proportioning beta-cyclodextrin is added the gauge water heating for dissolving, add the maltose dissolving then, solution is cooled to 65-70 ℃, and adjusting the pH value is 4.0-4.5, adds Pullulanase reaction 60 hours, be heated to 100 ℃, 15 minutes enzymes that go out adopt the separation and purification of SephadexG25 gel column, 60 ℃ of vacuum-dryings, be dried to moisture content less than 13%, obtain the branching cd product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610098196 CN1974604A (en) | 2006-12-08 | 2006-12-08 | Prepn process of branched cyclodextrin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610098196 CN1974604A (en) | 2006-12-08 | 2006-12-08 | Prepn process of branched cyclodextrin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1974604A true CN1974604A (en) | 2007-06-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610098196 Pending CN1974604A (en) | 2006-12-08 | 2006-12-08 | Prepn process of branched cyclodextrin |
Country Status (1)
| Country | Link |
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| CN (1) | CN1974604A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010139100A1 (en) * | 2009-05-31 | 2010-12-09 | 江南大学 | A method for producing maltotriose-beta-cyclodextrin by enzymic method |
| CN106520734A (en) * | 2016-11-28 | 2017-03-22 | 山东正德食品有限公司 | Method for improving heat resistance of pullulanase for catalyzing maltosyl-beta-cyclodextrin synthesis |
| CN106749772A (en) * | 2016-11-28 | 2017-05-31 | 山东正德食品有限公司 | A kind of purification process of maltose beta cyclodextrin |
| CN106755202A (en) * | 2016-11-28 | 2017-05-31 | 山东正德食品有限公司 | A kind of preparation method of maltose beta cyclodextrin |
| CN107177647A (en) * | 2017-05-16 | 2017-09-19 | 江南大学 | It is a kind of to be enzymatically treated the method and its application that maltodextrin prepares branched cyclodextrin |
| CN114343144A (en) * | 2022-01-18 | 2022-04-15 | 西南大学 | Method for improving hygroscopicity of jujube powder |
-
2006
- 2006-12-08 CN CN 200610098196 patent/CN1974604A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010139100A1 (en) * | 2009-05-31 | 2010-12-09 | 江南大学 | A method for producing maltotriose-beta-cyclodextrin by enzymic method |
| CN106520734A (en) * | 2016-11-28 | 2017-03-22 | 山东正德食品有限公司 | Method for improving heat resistance of pullulanase for catalyzing maltosyl-beta-cyclodextrin synthesis |
| CN106749772A (en) * | 2016-11-28 | 2017-05-31 | 山东正德食品有限公司 | A kind of purification process of maltose beta cyclodextrin |
| CN106755202A (en) * | 2016-11-28 | 2017-05-31 | 山东正德食品有限公司 | A kind of preparation method of maltose beta cyclodextrin |
| CN106749772B (en) * | 2016-11-28 | 2019-04-12 | 山东正德食品有限公司 | A kind of purification process of maltose-beta cyclodextrin |
| CN107177647A (en) * | 2017-05-16 | 2017-09-19 | 江南大学 | It is a kind of to be enzymatically treated the method and its application that maltodextrin prepares branched cyclodextrin |
| CN107177647B (en) * | 2017-05-16 | 2019-11-26 | 江南大学 | A kind of enzymatic treatment maltodextrin prepares the method and its application of branched cyclodextrin |
| CN114343144A (en) * | 2022-01-18 | 2022-04-15 | 西南大学 | Method for improving hygroscopicity of jujube powder |
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Open date: 20070606 |