CN1952163A - Process for preparing ethanol from stem and leaf of jerusalem artichoke - Google Patents
Process for preparing ethanol from stem and leaf of jerusalem artichoke Download PDFInfo
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Abstract
This invention involves a method for producing alcohol with Jerusalem artichoke stem tuber, stalk and leaves as raw materials, the implementation steps are as follows: crashing Jerusalem artichoke stem tuber, stalk, and leaves, adding water at a ratio of 1:0.75-2, cooking at 55-70 Deg C for 20-40 minutes, cooling to 30-45 Deg C, adding multiple Complex Phosphoesterasum containing 100-1000mg/kg cellulase, 100-500mg/kg hemicellulase, 100-500mg/kg Ligninase, digesting and transforming to obtain fermented mash; performing complex yeast alcohol fermentation by adding Kluyveromyces brittle wall yeast and ethanol active dry yeast or Kluyveromyces brittle wall yeast and wine active dry yeast and distilling the fermented mash to obtain ethanol. The invention with Jerusalem artichoke stem tuber, stalk and leaves as raw materials instead of food crops develops new resources and expand a novel way with broad market for the ethanol industry. The invention with simple method, extensive availability of raw materials, low prices can reduce production costs effectively, and is easy to form scaled production.
Description
Technical field
The present invention relates to produce the alcoholic acid method, especially relating to a kind of is raw material production alcoholic acid method with jerusalem artichoke stem tuber and cauline leaf.
Background technology
At present, ethanol industry is one of leading industry of China's fermentation industry, is mainly used in assembled alcoholic drinks, medicine, chemical industry.Along with the aggravation of world energy sources disparities between supply and demand, biomass energy particularly alcohol fuel has been listed in the future plan of China, and the present raw materials for production of China mainly are starchy material and molasses, synthetics etc.Starchy material ethanol accounts for 75%, and molasses ethanol accounts for 15%, and synthesizing alcohol accounts for 10%.Main starchy material is corn, Ipomoea batatas, cassava, and corn, Ipomoea batatas belong to food crop.Because China is as the maximum country of world population, for guaranteeing staple food supply, put into effect the policy of a series of restriction food crop system wine, therefore replace food crop with cash crop and produce ethanol, being the only way of China's ethanol industry development, equally also is further to push the very competitive scientific research project in world market to.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, is the present situation of raw material production with food crop mainly at present alcohol production, and providing a kind of is raw material production alcoholic acid method with jerusalem artichoke stem tuber and cauline leaf.
Implementation of the present invention is as follows for achieving the above object:
A kind of is raw material production alcoholic acid method with jerusalem artichoke stem tuber and cauline leaf, and implementation step is as follows:
(1) jerusalem artichoke and jerusalem artichoke cauline leaf process are pulverized, by 1: 0.75-2 adds water, after under the 55-70 ℃ of low temperature boiling 20-40 minute, be cooled to 30-45 ℃, adding cellulase 100-1000mg/kg, hemicellulase 100-500mg/kg, many kinds of prozymes of lignoenzyme 100-500mg/kg carry out enzymolysis, conversion to raw material, obtain karusen;
(2) Crewe dimension saccharomyces fragilis in 28 ℃, was cultivated 4-5 days on the wort slant medium, got a ring bacterial classification and was connected in the 500ml triangular flask that the 50-100ml seed culture medium is housed; The shake-flask seed substratum consists of (g/L): sucrose: 3.0-30, peptone: 1.0-2.0, yeast extract paste: 1.0-2.0, MgSO
47H
2O:0.1-2.0, K
2HPO
4: 0.3-3.0, NaCl:0.1-1.5; PH:4.0-5.0, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 28-35 ℃, rotating speed is to cultivate 12-24 hour on 200-300 rev/min the shaking table, as primary seed solution;
(3) getting the access of 5-10ml primary seed solution is equipped with in the 500ml triangular flask of 50-100ml seed culture medium; The shake-flask seed substratum is formed the same step of proportioning (2), pH:4.0-5.0, and in 121 ℃ of sterilizations, 25 minutes, shake bottle in 28-35 ℃, rotating speed is to cultivate 12-24 hour on 200-300 rev/min the shaking table, as secondary seed solution;
(4) inoculum size by 5-10% inserts secondary seed in the fermentor tank; The seed culture medium that in fermentor tank, adds the 65-70% volume, it forms identical with above-mentioned shake-flask seed substratum composition proportioning; PH:4.0-5.0 was in 121 ℃ of sterilizations, 25 minutes; In 28-35 ℃, air flow 0.5-1.5V/ (Vmin), stirring velocity 200-300 rev/min of condition bottom fermentation 16-24 hour, grain weight after the enlarged culturing, obtains Crewe dimension saccharomyces fragilis liquid distiller's yeast step by step as required;
(5) add Crewe dimension saccharomyces fragilis and ethanol active dry yeast, or Crewe is tieed up saccharomyces fragilis and wine active dry yeast carries out the composite yeast ethanol fermentation; Promptly ethanol or the wine active dry yeast access karusen by 5-10% liquid distiller's yeast and 0.1-0.5% carries out ethanol fermentation, and 30-33 ℃ of fermentation finished after 48-72 hour, obtained sophisticated ethanol fermentation wine with dregs at last and was used to distill ethanol.
The invention has the beneficial effects as follows: jerusalem artichoke (Jerusalem artichoke) is the tuberous plant of underground growth, and cultivation easily can annual be gathered in the crops after the plantation in a year, can even receive 4-5.Because its outer likeness in form ginger is so have another name called Jerusalem artichoke, Jerusalem artichoke.Jerusalem artichoke stem, leaf, root all contain inulin.The jerusalem artichoke root contains 80% moisture, and all the other solid substances of 20% are by 90% carbohydrate, 6% Mierocrystalline cellulose, hemicellulose, 2% protein and 2% ash composition.The contained carbohydrate of the cauline leaf of jerusalem artichoke is than contained less slightly of rhizome, greatly about about 10-12%.On average can gather in the crops 3 tons of jerusalem artichoke roots every mu of every year in China, and 7 tons of cauline leafs, so the jerusalem artichoke producing and ethanol in next life that can be fully utilized, its residuum also are the good raw materials of producing nutritious animal-feed.China has put into effect the policy of a series of restriction grain system wine, therefore replaces food crop with jerusalem artichoke and jerusalem artichoke cauline leaf and produces ethanol, effectively develops new resource, will be China and even new road of world's ethanol industry development developing.Production method of the present invention is simple, and raw material sources are wide, price is low, can effectively reduce production costs, and easily form large-scale production.
Embodiment
Below in conjunction with preferred embodiment, to details are as follows according to embodiment provided by the invention:
Embodiment 1
(1) jerusalem artichoke and jerusalem artichoke cauline leaf are through pulverizing, added entry by 1: 2.0,65 ℃ of low temperature boilings were cooled to 45 ℃ after 30 minutes, adding cellulase 1000mg/kg, multiple prozymes such as hemicellulase 500mg/kg, lignoenzyme 500mg/kg carry out enzymolysis, conversion to raw material, obtain karusen;
(2) Crewe dimension saccharomyces fragilis in 28 ℃ of cultivations 5 days, is got a ring bacterial classification and is connected in the 500ml triangular flask that the 100ml seed culture medium is housed on the wort slant medium; The shake-flask seed substratum consists of (g/L): sucrose: 30.0, and peptone: 3.0, yeast extract paste: 1.5, MgSO
47H
2O:0.3, K
2HPO
4: 3.0, NaCl:1.0; PH:4.0 in 121 ℃ of sterilizations 25 minutes, shakes bottle in 32 ℃, and rotating speed is to cultivate 12 hours on 200 rev/mins the shaking table, as primary seed solution;
(3) getting the access of 10ml bacteria suspension is equipped with in the 500ml triangular flask of 100ml seed culture medium.The shake-flask seed substratum consists of (g/L): sucrose: 30.0, and peptone: 2.0, yeast extract paste: 2.0, MgSO
47H
2O:0.5, K
2HPO
4: 3.0, NaCl:1.0; PH:4.0, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 28 ℃, rotating speed is to cultivate 12 hours on 200 rev/mins the shaking table, as secondary seed solution;
(4) by 10% inoculum size secondary seed is inserted in the fermentor tank.Add the seed culture medium of 65% volume in fermentor tank, seed culture medium consists of (g/L): sucrose: 30.0, and peptone: 1.5, yeast extract paste: 1.5, MgSO
47H
2O:2.0, K
2HPO
4: 3.0, NaCl:1.0; PH:4.0, in 121 ℃ of sterilizations, 25 minutes, in 35 ℃, air flow 1.5V/ (Vmin), 200 rev/mins of condition bottom fermentations of stirring velocity 24 hours after the enlarged culturing, obtain Crewe dimension saccharomyces fragilis liquid distiller's yeast step by step;
(5) the ethanol active dry yeast access karusen of 10% Crewe being tieed up saccharomyces fragilis liquid distiller's yeast and 0.5% carries out ethanol fermentation, and 30 ℃ of fermentations finished after 48 hours, and sophisticated mash is sent to distillation.
Embodiment 2
(1) jerusalem artichoke and jerusalem artichoke cauline leaf through pulverizing, by adding entry at 1: 1.5,60 ℃ of low temperature boilings are after 25 minutes, be cooled to 30 ℃, adding cellulase 100mg/kg, multiple prozymes such as hemicellulase 100mg/kg, lignoenzyme 100mg/kg carry out enzymolysis, conversion to raw material, obtain karusen;
(2) Crewe dimension saccharomyces fragilis in 28 ℃ of cultivations 4 days, is got a ring bacterial classification and is connected in the 500ml triangular flask that the 100ml seed culture medium is housed on the wort slant medium.The shake-flask seed substratum consists of (g/l): sucrose: 30.0, and peptone: 1.0, yeast extract paste: 2.0, MgSO
47H
2O:2.0, K
2HPO
4: 2.5, NaCl:1.5; PH:4.5, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 32 ℃, rotating speed is to cultivate 12 hours on 300 rev/mins the shaking table, as primary seed solution;
(3) getting the access of 10ml bacteria suspension is equipped with in the 500ml triangular flask of 100ml seed culture medium.The shake-flask seed substratum consists of (g/L): sucrose: 30.0, and peptone: 1.0, yeast extract paste: 2.0, MgSO
47H
2O:2.0, K
2HPO
4: 2.5, NaCl:1.5; PH:4.5, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 32 ℃, rotating speed is to cultivate 12 hours on 300 rev/mins the shaking table, as secondary seed solution;
(4) by 10% inoculum size secondary seed is inserted in the fermentor tank.Add the seed culture medium of 70% volume in fermentor tank, seed culture medium consists of (g/L): sucrose: 30.0, and peptone: 1.0, yeast extract paste: 2.0, MgSO
47H
2O:0.5, K
2HPO
4: 2.5, NaCl:1.0; PH:4.5 was in 121 ℃ of sterilizations, 25 minutes; In 28 ℃, air flow 0.75V/ (Vmin), 300 rev/mins of condition bottom fermentations of stirring velocity 24 hours after the enlarged culturing, obtain Crewe dimension saccharomyces fragilis liquid distiller's yeast step by step;
(5) the Shandong dimension saccharomyces fragilis liquid distiller's yeast of 5% gram and 0.1% ethanol active dry yeast are inserted karusen, in fermentor tank, carry out ethanol fermentation, 33 ℃ of fermentations after 24 hours are told karusen 1/3-1/2 to second jar, right latter two jar is added sweet mash simultaneously to full, continues fermentation; Treat that second jar of fermentation is normal, when being in main fermentation stage again, tell three jars of 1/3-1/2 karusens to the again simultaneously, and add sweet mash to second and third jar.Cut apart third and fourth so continuously ... each jar; First and second of front jar fermentation is after 60 hours, and sophisticated mash is sent to distillation.
Embodiment 3
(1) jerusalem artichoke and jerusalem artichoke cauline leaf through pulverizing, by adding entry at 1: 1.25,70 ℃ of low temperature boilings are after 20 minutes, be cooled to 30-45 ℃, adding cellulase 500mg/kg, multiple prozymes such as hemicellulase 200mg/kg, wooden plain enzyme 200mg/kg carry out enzymolysis, conversion to raw material, obtain karusen;
(2) Crewe dimension saccharomyces fragilis in 28 ℃ of cultivations 5 days, is got a ring bacterial classification and is connected in the 500ml triangular flask that the 100ml seed culture medium is housed on the wort slant medium.The shake-flask seed substratum consists of (g/L): sucrose: 3.0, and peptone: 2.0, yeast extract paste: 1.0, MgSO
47H
2O:0.1, K
2HPO
4: 0.3, NaCl:0.1; PH:5.0, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 32 ℃, rotating speed is to cultivate 12 hours on 200 rev/mins the shaking table, as primary seed solution;
(3) getting the access of 10ml bacteria suspension is equipped with in the 500ml triangular flask of 100ml seed culture medium.The shake-flask seed substratum consists of (g/L): sucrose: 25.0, and peptone: 2.0, yeast extract paste: 1.0, MgSO
47H
2O:0.1, K
2HPO
4: 0.3, NaCl:0.1; PH:5.0, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 32 ℃, rotating speed is to cultivate 12 hours on 200 rev/mins the shaking table, as secondary seed solution;
(4) by 10% inoculum size secondary seed is inserted in the fermentor tank.Add the seed culture medium of 70% volume in fermentor tank, seed culture medium consists of (g/L): sucrose: 25.0, and peptone: 2.0, yeast extract paste: 1.0, MgSO
47H
2O:0.1, K
2HPO
4: 0.3, NaCl:0.1; PH:5.0 was in 121 ℃ of sterilizations, 25 minutes; In 30 ℃, air flow 1.0V/ (Vmin), 200 rev/mins of condition bottom fermentations of stirring velocity 24 hours after the enlarged culturing, obtain Crewe dimension saccharomyces fragilis liquid distiller's yeast step by step;
(5) the wine active dry yeast access karusen of 5% Crewe being tieed up saccharomyces fragilis liquid distiller's yeast and 0.3% carries out ethanol fermentation, and 31 ℃ of fermentations finished after 72 hours, and sophisticated mash is sent to distillation.
Embodiment 4
(1) jerusalem artichoke and jerusalem artichoke cauline leaf through pulverizing, by adding entry at 1: 0.75,55 ℃ of low temperature boilings are after 40 minutes, be cooled to 30-45 ℃, adding cellulase 600mg/kg, multiple prozymes such as hemicellulase 300mg/kg, wooden plain enzyme 300mg/kg carry out enzymolysis, conversion to raw material, obtain karusen;
(2) Crewe dimension saccharomyces fragilis in 28 ℃ of cultivations 4 days, is got a ring bacterial classification and is connected in the 500ml triangular flask that the 100ml seed culture medium is housed on the wort slant medium.The shake-flask seed substratum consists of (g/L): sucrose: 3.0, and peptone: 2.0, yeast extract paste: 1.0, MgSO
47H
2O:0.1, K
2HPO
4: 0.3, NaCl:0.1; PH:5.0, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 32 ℃, rotating speed is to cultivate 12 hours on 250 rev/mins the shaking table, as primary seed solution;
(3) getting the access of 10ml bacteria suspension is equipped with in the 500ml triangular flask of 100ml seed culture medium.The shake-flask seed substratum consists of (g/L): sucrose: 3.0, and peptone: 2.0, yeast extract paste: 1.0, MgSO
47H
2O:0.1, K
2HPO
4: 0.3, NaCl:0.1; PH:5.0 in 121 ℃ of sterilizations 25 minutes, shakes bottle in 32 ℃, and rotating speed is to cultivate 12 hours on 250 rev/mins the shaking table, as secondary seed solution;
(4) by 10% inoculum size shake-flask seed is inserted in the fermentor tank.Add 67.5% seed culture medium in fermentor tank, seed culture medium consists of (g/L): sucrose: 20.0, and peptone: 2.0, yeast extract paste: 1.0, MgSO
47H
2O:0.1, K
2HPO
4: 0.3, NaCl:0.1; PH:5.0 was in 121 ℃ of sterilizations, 25 minutes; In 31 ℃, air flow 1.2V/ (Vmin), 250 rev/mins of condition bottom fermentations of stirring velocity 24 hours after the enlarged culturing, obtain Crewe dimension saccharomyces fragilis liquid distiller's yeast step by step;
(5) wine active dry yeast of 10% Crewe being tieed up saccharomyces fragilis liquid distiller's yeast and 0.5% inserts karusen, in fermentor tank, carry out ethanol fermentation, 30 ℃ of fermentations after 24 hours are told karusen 1/3-1/2 to second jar, right latter two jar is added sweet mash simultaneously to full, continues fermentation; Treat that second jar of fermentation is normal, when being in main fermentation stage again, tell three jars of 1/3-1/2 karusens to the again simultaneously, and add sweet mash to second and third jar; Cut apart third and fourth so continuously ... each jar.First and second of front jar fermentation is after 70 hours, and sophisticated mash is sent to distillation.
Above-mentioned with reference to embodiment to a kind of be the detailed description that raw material production alcoholic acid method is carried out with jerusalem artichoke stem tuber and cauline leaf; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (1)
1, a kind of is raw material production alcoholic acid method with jerusalem artichoke stem tuber and cauline leaf, and implementation step is as follows:
(1) jerusalem artichoke and jerusalem artichoke cauline leaf process are pulverized, by 1: 0.75-2 adds water, after under the 55-70 ℃ of low temperature boiling 20-40 minute, be cooled to 30-45 ℃, adding cellulase 100-1000mg/kg, hemicellulase 100-500mg/kg, many kinds of prozymes of lignoenzyme 100-500mg/kg carry out enzymolysis, conversion to raw material, obtain karusen;
(2) Crewe dimension saccharomyces fragilis in 28 ℃, was cultivated 4-5 days on the wort slant medium, got a ring bacterial classification and was connected in the 500ml triangular flask that the 50-100ml seed culture medium is housed; The shake-flask seed substratum consists of (g/L): sucrose: 3.0-30, peptone: 1.0-2.0, yeast extract paste: 1.0-2.0, MgSO
47H
2O:0.1-2.0, K
2HPO
4: 0.3-3.0, NaCl:0.1-1.5; PH:4.0-5.0, in 121 ℃ of sterilizations, 25 minutes, shake bottle in 28-35 ℃, rotating speed is to cultivate 12-24 hour on 200-300 rev/min the shaking table, as primary seed solution;
(3) getting the access of 5-10ml primary seed solution is equipped with in the 500ml triangular flask of 50-100ml seed culture medium; The shake-flask seed substratum is formed the same step of proportioning (2), pH:4.0-5.0, and in 121 ℃ of sterilizations, 25 minutes, shake bottle in 28-35 ℃, rotating speed is to cultivate 12-24 hour on 200-300 rev/min the shaking table, as secondary seed solution;
(4) inoculum size by 5-10% inserts secondary seed in the fermentor tank; The seed culture medium that in fermentor tank, adds the 65-70% volume, it forms identical with above-mentioned shake-flask seed substratum composition proportioning; PH:4.0-5.0 was in 121 ℃ of sterilizations, 25 minutes; In 28-35 ℃, air flow 0.5-1.5V/ (Vmin), stirring velocity 200-300 rev/min of condition bottom fermentation 16-24 hour, grain weight after the enlarged culturing, obtains Crewe dimension saccharomyces fragilis liquid distiller's yeast step by step as required;
(5) add Crewe dimension saccharomyces fragilis and ethanol active dry yeast, or Crewe is tieed up saccharomyces fragilis and wine active dry yeast carries out the composite yeast ethanol fermentation; Promptly ethanol or the wine active dry yeast access karusen by 5-10% liquid distiller's yeast and 0.1-0.5% carries out ethanol fermentation, and 30-33 ℃ of fermentation finished after 48-72 hour, obtained sophisticated ethanol fermentation wine with dregs at last and was used to distill ethanol.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845462A (en) * | 2010-05-27 | 2010-09-29 | 山西金绿禾燕麦研究所 | Method for producing alcohol by using stalks of jerusalem artichoke as raw material and adopting immobilized yeast fermentation of inulin |
| CN101619328B (en) * | 2009-06-02 | 2012-02-15 | 大连理工大学 | Method for fermenting and producing 2,3-butanediol by utilizing tuber and stem leaf of jerusalem artichoke as raw materials |
| CN101532036B (en) * | 2008-03-11 | 2012-03-14 | 浙江杭州鑫富药业股份有限公司 | Method for producing butane diacid by fermenting Jerusalem artichoke raw material |
| CN102864174A (en) * | 2011-07-04 | 2013-01-09 | 华东理工大学 | Method for producing high density ethanol without enzyme addition by taking inulin biomass as raw material |
| CN105200094A (en) * | 2014-05-30 | 2015-12-30 | 中粮营养健康研究院有限公司 | Method for producing ethanol from microbial fermentation lignocellulose raw material |
| CN105238642A (en) * | 2015-11-10 | 2016-01-13 | 江苏碧青园海洋生物科技有限公司 | Brewing method of low-alcohol Jerusalem artichoke health-care wine |
| CN105419841A (en) * | 2015-12-03 | 2016-03-23 | 高大元 | Method for preparing jerusalem artichoke alcohol pyrolyzed enteromorpha renewable energy bio-oil |
| CN107881052A (en) * | 2017-11-08 | 2018-04-06 | 深圳瑞德源健康科技有限公司 | A kind of feature jerusalem artichoke fruit wine and preparation method thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2144084C1 (en) * | 1998-11-06 | 2000-01-10 | Московский Государственный Университет пищевых производств | Method of production of ethyl alcohol from jerusalem artichoke |
| RU2161652C1 (en) * | 2000-02-08 | 2001-01-10 | Московский Государственный Университет пищевых производств | Method of production of ethyl alcohol from jerusalem artichoke |
| CN100422338C (en) * | 2005-04-08 | 2008-10-01 | 江苏天士力帝益药业有限公司 | Process of extracting synanthrin from girasole |
| CN1847266A (en) * | 2005-04-15 | 2006-10-18 | 三达工业技术（厦门）有限公司 | Girasole synanthrin producing process based on two-stage ultrafiltering technology |
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- 2006-11-03 CN CN2006101292055A patent/CN1952163B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101532036B (en) * | 2008-03-11 | 2012-03-14 | 浙江杭州鑫富药业股份有限公司 | Method for producing butane diacid by fermenting Jerusalem artichoke raw material |
| CN101619328B (en) * | 2009-06-02 | 2012-02-15 | 大连理工大学 | Method for fermenting and producing 2,3-butanediol by utilizing tuber and stem leaf of jerusalem artichoke as raw materials |
| CN101845462A (en) * | 2010-05-27 | 2010-09-29 | 山西金绿禾燕麦研究所 | Method for producing alcohol by using stalks of jerusalem artichoke as raw material and adopting immobilized yeast fermentation of inulin |
| CN102864174A (en) * | 2011-07-04 | 2013-01-09 | 华东理工大学 | Method for producing high density ethanol without enzyme addition by taking inulin biomass as raw material |
| CN102864174B (en) * | 2011-07-04 | 2014-09-03 | 华东理工大学 | Method for producing high density ethanol without enzyme addition by taking inulin biomass as raw material |
| CN105200094A (en) * | 2014-05-30 | 2015-12-30 | 中粮营养健康研究院有限公司 | Method for producing ethanol from microbial fermentation lignocellulose raw material |
| CN105200094B (en) * | 2014-05-30 | 2019-12-03 | 中粮营养健康研究院有限公司 | A method of utilizing microbial fermentation lignocellulosic material producing and ethanol |
| CN105238642A (en) * | 2015-11-10 | 2016-01-13 | 江苏碧青园海洋生物科技有限公司 | Brewing method of low-alcohol Jerusalem artichoke health-care wine |
| CN105419841A (en) * | 2015-12-03 | 2016-03-23 | 高大元 | Method for preparing jerusalem artichoke alcohol pyrolyzed enteromorpha renewable energy bio-oil |
| CN107881052A (en) * | 2017-11-08 | 2018-04-06 | 深圳瑞德源健康科技有限公司 | A kind of feature jerusalem artichoke fruit wine and preparation method thereof |
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| Publication number | Publication date |
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| CN1952163B (en) | 2011-05-04 |
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