CN103614448B - Method for preparing bioethanol by taking sodium alginate or algae as active ingredients - Google Patents
Method for preparing bioethanol by taking sodium alginate or algae as active ingredients Download PDFInfo
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- CN103614448B CN103614448B CN201310606281.0A CN201310606281A CN103614448B CN 103614448 B CN103614448 B CN 103614448B CN 201310606281 A CN201310606281 A CN 201310606281A CN 103614448 B CN103614448 B CN 103614448B
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Abstract
The invention discloses a method for preparing bioethanol by taking sodium alginate or algae as active ingredients. Fermentation culture medium taking sodium alginate as a carbon source can be adopted, or the fermentation culture medium taking algae treatment liquor as a carbon source can be adopted, the algae treatment liquor is obtained by adopting the following method: mixing algae powder with water, and sterilizing; mixing algae powder with citric acid-sodium citrate buffer solution, regulating the pH value to be neutral with NaOH solution, adding cellulase which is 10% of the mass of algae powder, performing enzymolysis so that the cellulase is inactivated; mixing algae powder with aqueous sulfuric acid solution, and hydrolyzing. The method for preparing bioethanol comprises the steps of: inoculating 5% Meyerozyma guilliermondii strain seed solution into a sterilized fermentation culture medium, multiplying, then performing airtight anaerobic fermentation for 5-7 days at 28-32DEG C; distilling the fermented liquid to obtain bioethanol. According to the method, the bioethanol can be produced by utilizing marine algae resources; therefore, the commercial crops of grains and the like can be saved, cultivated land cannot be occupied and fresh water resources cannot be consumed; remaining solids after fermentation can serve as organic fertilizers, and the algae utilization rate can be improved; the method is simple and easy, and absolutely capable of carrying out industrial production.
Description
Technical field
The present invention relates to a kind of preparation method of bio-ethanol, particularly with marine alga and Seaweed Extract for the method for bio-ethanol prepared by raw material.
Background technology
At present, petroleum resources deposit has arrived and has been on the verge of exhausted stage, and bio-ethanol becomes more effective substitute products, and bio-ethanol refers to that the biomass being rich in glucide produce ethanol through fermentable effect, can add in gasoline and make propellant combination.But fabricate fuel ethanol uses grain or the economic class crop such as corn, wheat, soybean, Semen Brassicae campestris, sugarcane mostly, from input, the input output analysis of the energy, this structure is uneconomic, has caused the dispute in global range.Other raw materials are difficult to form large-scale commercial production ability due to the restriction of arable land, technology etc.Marine algae resource because of its output huge, the high and high yield of photosynthetic efficiency and become desirable bio-ethanol raw materials for production.Lalgine, also known as alginic acid, Lalgine, seaweeds, is a kind of natural polysaccharide be present in alginic cell wall.Monomer is beta-D-mannuronic acid (M) and α-L-guluronic acid (G).M and G unit becomes segmented copolymer with the array mode of M-M, G-G or M-G by Isosorbide-5-Nitrae glycosidic link is connected.Lalgine (accounting for dry cell weight 40%) as main sugar in marine alga but can not as marine alga starch, mannitol, and by alcohol production bacterium metabolism such as yeast, this causes the waste of biomass.In order to improve alcohol yied, build multiple genetically engineered bacterial classification that effectively can utilize Lalgine fermentative production of ethanol, the people such as Hiroyuki Takeda construct a kind of Lalgine metabolism bacterium Sphingomonas sp.A1, this bacterial classification contains the alcohol metabolism approach of manual construction, can take Lalgine as sole carbon source, after fermentation 72h, alcohol concn reaches 13g/L.But due to deficient in stability in industrial fermentation situation, heredity and metabolic process are difficult to control, and lack the method etc. of this primeval life being carried out to genetic modification simultaneously, this project bacterium is applied to large scale fermentation production ethanol and seems unlikely.
Summary of the invention
The object of the present invention is to provide a kind of method simply, to be not only suitable for suitability for industrialized production but effectively can utilize oceanic resources a kind of with sodium alginate or marine alga for the method for bio-ethanol prepared by raw material.The present invention is mainly: the bacterial classification utilizing sodium alginate fermentative production of ethanol, take marine alga as substrate raw material, under the control of the key parameters such as temperature, pH, inoculum size and fermentation time, by single strain fermentation and the mixed fermentation effect of microorganism, carbohydrate fermentation in marine alga is utilized to produce ethanol.
One, raw material:
1, sodium alginate (food grade), it directly can prepare ethanol.
2, marine alga (as sea-tangle or wakame) needs to carry out pre-treatment, to increase the sugared content in fermented liquid, and then prepares ethanol, improves the utilization ratio of marine alga.The pre-treatment of marine alga: first dry seaweed is removed impurity, obtains qualified seaweed powder through 100 mesh sieves after pulverizing, then seaweed powder is made marine alga treatment solution, and its mode has three kinds:
1) seaweed powder is mixed with its quality 30-50 water doubly, boiling 20-30min at sterilizing 15min or 90-100 DEG C at 110 DEG C-115 DEG C; Marine alga treatment solution is obtained after cooling;
2) the citric acid-sodium citrate damping fluid of seaweed powder with its quality 30 times is mixed, by 2mol/L NaOH solution adjust ph to neutral, add the cellulase of seaweed powder quality 1/10th, enzymolysis 3h at 40 DEG C, pH is adjusted to about 5 with 2mol/L HCl after enzymolysis completes, marine alga liquid after enzymolysis is in 121 DEG C, and 15min sterilizing makes enzyme deactivation simultaneously, obtains marine alga treatment solution after cooling;
3) by seaweed powder and with its quality 20-30 doubly containing 1%(mass concentration) aqueous solution of sulfuric acid, be hydrolyzed 20min-30min at 121 DEG C, after cooling marine alga treatment solution.
Two, preparation method
1, with the technical process that ethanol prepared by sodium alginate be:
(1) the fermention medium ((NH of preparation using sodium alginate as carbon source
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, sodium alginate 20-25g/L), natural ph, not adjusted to ph in fermenting process, at 121 DEG C, sterilizing 15min, is cooled to room temperature;
(2) add the cultivation Meyerozyma guilliermondii bacterial classification seed liquor of 24 hours with 5% inoculation quality than in the solution of step (1), mix, then in 30 DEG C, under 150rpm condition, cultivate 24h;
(3) at 28-32 DEG C, sealing anaerobically fermenting, fermentation time is 5-7 days, in fermenting process not adjust ph but exhaust;
(4) after getting fermentation, liquid obtains bio-ethanol by distillation.
2, with the technical process that ethanol prepared by marine alga pretreatment fluid be:
(1) with the marine alga treatment solution fermention medium as carbon source, it consists of (NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, marine alga treatment solution 20g/L, with 2mol/L NaOH solution or 2mol/L HCl solution adjusted to ph 4.5-5.5;
(2) be 5% access Meyerozyma guilliermondii bacterial classification seed liquor by inoculation quality ratio in the solution of step (1), best, this bacterial classification seed liquor and Saccharomyces cerevisiae bacterial classification seed liquor form mixed strains, the inoculation quality of this both mixed strains is than being Meyerozyma guilliermondii:Saccharomyces cerevisiae=4:1:, mix, then in 30 DEG C, 150rpm cultivates 24h;
(3) at 28-32 DEG C, anaerobically fermenting is 5 days, in fermenting process not adjust ph but exhaust;
(4) after getting fermentation, liquid is by obtaining ethanol by distillation; Distillation residual solution is after solid-liquid separation, and solids can be used as fertilizer.
The present invention compared with prior art tool has the following advantages:
(1) the present invention not only can utilize Lalgine fermentative production of ethanol, but also marine algae resource can be utilized to produce bio-ethanol, both saved the cash crop such as grain, do not occupy cultivated land again and Freshwater resources, remaining solid simultaneously after fermentation can be used as organic fertilizer, improves marine alga utilization ratio.
(2) utilize multiple bacterial classification to carry out single strain fermentation, fermented by mixed bacterium, effectively can utilize different components substrate, effectively improve alcohol yied.
(3) method of the present invention is simple, completely can suitability for industrialized production.
Embodiment
Embodiment 1
Get the fermention medium that 100g sodium alginate is prepared as carbon source, this fermention medium is composed as follows: (NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, sodium alginate 20g/L, pH value=6, at 121 DEG C, sterilizing 15min, is cooled to room temperature; Access Meyerozyma guilliermondii bacterial classification seed liquor with 5% inoculation quality than in aforesaid liquid, mix, then in 30 DEG C, 150rpm carries out breeding and cultivates 24h; At 28-32 DEG C, sealing anaerobically fermenting is cultivated, and connects airway, and the other end inserts in water, enters in fermenting container to prevent air.Fermentation time is 5 days, not adjust ph in fermenting process, but needs Exhaust Gas; Separating liquid obtains 15.4g ethanol by distillation, and productive rate is 0.154g ethanol/g sodium alginate.
Embodiment 2
Dry sea-tangle surface impurity is removed, after pulverizing with pulverizer, crosses 100 mesh sieves; Get 20g Kelp Powder, with 600mL(30 doubly) clear water mix, sterilizing 15min at 110 DEG C-115 DEG C, must marine alga treatment solution after cooling; Sodium alginate preparation fermention medium ((NH is replaced with sea-tangle hydrolyzate
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, above-mentioned sea-tangle treatment solution), regulate fermention medium pH value to be 5 with 2mol/L HCl solution; 115 DEG C of sterilizing 15min, access Meyerozyma guilliermondii bacterial classification seed liquor with 5% inoculation quality than in liquid after being cooled to room temperature, mix, and then in 30 DEG C, 150rpm carries out single culture propagation and cultivates 24h; Connect airway, the other end inserts in water, and then at 28-32 DEG C, sealing anaerobically fermenting cultivates 5 days, not adjust ph in fermenting process, but needs exhaust; Get fermented liquid after having fermented and obtain 4.74g ethanol by distillation, productive rate is 0.237g ethanol/g sea-tangle; Distillation residual solution is after solid-liquid separation, and solids can be used as fertilizer.
Embodiment 3
Dry sea-tangle surface impurity is removed, after pulverizing with pulverizer, crosses 100 mesh sieves; Get 20g Kelp Powder and 600mL(30 doubly) clear water mix, boiling 20-30min at 90-100 DEG C, and must marine alga treatment solution after cooling; Sodium alginate is replaced, preparation fermention medium ((NH with sea-tangle hydrolyzate
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, above-mentioned sea-tangle treatment solution), adjusting fermention medium pH with 2mol/L HCl is 5,115 DEG C of sterilizing 15min, access the seed liquor of Meyerozymaguilliermondii bacterial classification and Saccharomyces cerevisiae after being cooled to room temperature than in fermented liquid with 5% inoculation quality, their ratio is 4:1, mixes, then in 30 DEG C, 150rpm carries out mixed strains breeding and cultivates 24h; Connect airway, the other end inserts in water, and then at 28-32 DEG C, sealing anaerobically fermenting cultivates 5 days, not adjust ph in fermenting process, but needs Exhaust Gas; Get fermented liquid after having fermented and obtain 4.90g ethanol through distillation, productive rate is 0.245g ethanol/g sea-tangle; Residual solid can be used as organic fertilizer.
Embodiment 4
Dry sea-tangle surface impurity is removed, after pulverizing with pulverizer, crosses 100 mesh sieves; Get 20g Kelp Powder and 600mL(30 doubly) citric acid-sodium citrate damping fluid mix, by 2mol/L NaOH solution adjust ph to neutrality, adds the cellulase of Kelp Powder quality 1/10th, enzymolysis 3h at 40 DEG C; After enzymolysis completes, preparation fermention medium ((NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, above-mentioned sea-tangle treatment solution), adjust pH to 5 with 2mol/L HCl, 121 DEG C, 15min sterilizing makes enzyme deactivation simultaneously, obtains sea-tangle treatment solution after cooling; With the seed liquor of 5% inoculation quality than access Meyerozyma guilliermondii bacterial classification, mix, then in 30 DEG C, 150rpm carries out single culture propagation and cultivates 24h; Connect airway, the other end inserts in water, at 28-32 DEG C, and sealing anaerobically fermenting 5 days, not adjust ph in fermenting process, but need exhaust; Get fermented liquid after having fermented and obtain 4.84g ethanol through distillation, productive rate is 0.242g ethanol/g sea-tangle; Residual solid can be used as organic fertilizer.
Embodiment 5
Dry sea-tangle surface impurity is removed, after pulverizing with pulverizer, crosses 100 mesh sieves; Get 20g Kelp Powder and 600mL(30 doubly) citric acid-sodium citrate damping fluid mix, by 2mol/L NaOH solution adjust ph to neutrality, adds the cellulase of Kelp Powder quality 1/10th, enzymolysis 3h at 40 DEG C; Preparation fermention medium ((NH after enzymolysis completes
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, above-mentioned sea-tangle treatment solution), adjust pH to 4.5 with 2mol/L HCl, 121 DEG C, 15min sterilizing makes enzyme deactivation simultaneously, obtains marine alga treatment solution after cooling; With 5% inoculation quality than the seed liquor accessing Meyerozyma guilliermondii and Saccharomyces cerevisiae bacterial classification, their ratio is 4:1, mixes, and then in 30 DEG C, 150rpm carries out mixed strains breeding and cultivates 24h; Connect airway, the other end inserts in water, at 28-32 DEG C, and sealing anaerobically fermenting 5 days, not adjust ph in fermenting process, but need exhaust; Get fermented liquid after having fermented and obtain 5.02g ethanol through distillation, productive rate is 0.251g ethanol/g sea-tangle, and residual solid can be used as organic fertilizer.
Embodiment 6
Dry sea-tangle surface impurity is removed, pulverized 100 mesh sieves with pulverizer; Get the sulphuric acid soln mixing that 20g Kelp Powder and 500mL mass concentration are 1%, at 121 DEG C, heat 20min, after cooling marine alga treatment solution prepare fermention medium ((NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, above-mentioned sea-tangle treatment solution); By 2mol/L NaOH solution adjust ph to 5; Sterilizing 15min at 121 DEG C, after being cooled to room temperature, the inoculation quality with 5%, than the seed liquor adding Meyerozyma guilliermondii bacterial classification, mixes, and then in 30 DEG C, 150rpm carries out mixed strains breeding and cultivates 24h; Connect airway, the other end inserts in water, at 28-32 DEG C, and sealing anaerobically fermenting 5 days, not adjust ph in fermenting process, but need exhaust; Get fermented liquid after having fermented and obtain 4.16g ethanol through distillation, productive rate is 0.208g ethanol/g sea-tangle; Residual solid can be used as organic fertilizer.
Embodiment 7
Dry wakame surface impurity is removed, pulverized 100 mesh sieves with pulverizer; Get the sulphuric acid soln mixing that 20g Kelp Powder and 500mL mass concentration are 1%, at 121 DEG C, heat 20min, after cooling marine alga treatment solution prepare fermention medium ((NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o1.1g/L, above-mentioned wakame treatment solution); By 2mol/L NaOH solution adjust ph to 5.5; Sterilizing 15min at 121 DEG C, after being cooled to room temperature, inoculation quality with 5% is than the seed liquor adding Meyerozyma guilliermondii and Saccharomyces cerevisiae bacterial classification, their ratio is 4:1, mix, then in 30 DEG C, 150rpm carries out mixed strains breeding and cultivates 24h; Connect airway, the other end inserts in water, at 28-32 DEG C, and sealing anaerobically fermenting 5 days, not adjust ph in fermenting process, but need exhaust; Get fermented liquid after having fermented and obtain 4.62g ethanol through distillation, productive rate is 0.231g ethanol/g sea-tangle.Residual solid can be used as organic fertilizer.
Claims (3)
1. be the method that bio-ethanol prepared by raw material with sodium alginate, it is characterized in that:
(1) preparation take sodium alginate as the fermention medium of carbon source, it consists of (NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o 1.1g/L, sodium alginate 20-25g/L, natural ph, at 121 DEG C, sterilizing 15min, is cooled to room temperature;
(2) cultivating rear Meyerozyma guilliermondii bacterial classification seed liquor with 5% inoculation than adding in the solution of step (1), mixing, then in 30 DEG C, under 150rpm condition, cultivating 24h;
(3) at 28-32 DEG C, sealing anaerobically fermenting, fermentation time is 5-7 days, in fermenting process not adjust ph but exhaust;
(4) after getting fermentation, liquid obtains bio-ethanol by distillation.
2. be the method that bio-ethanol prepared by raw material with marine alga, it is characterized in that:
(1) marine alga pre-treatment:
First dry seaweed is removed impurity, cross 100 mesh sieves after pulverizing and obtain qualified seaweed powder, then seaweed powder is made marine alga treatment solution, its mode has following three kinds:
1) seaweed powder is mixed with its quality 30-50 water doubly, boiling 20-30min at sterilizing 15min or 90-100 DEG C at 110 DEG C-115 DEG C; Marine alga treatment solution is obtained after cooling,
Or 2) the citric acid-sodium citrate damping fluid of seaweed powder with its quality 30 times is mixed, by 2mol/L NaOH solution adjust ph to neutral, add the cellulase of seaweed powder quality 1/10th, enzymolysis 3h at 40 DEG C, preparation fermention medium after enzymolysis completes, adjusts pH to about 5 with 2mol/L HCl, then in 121 DEG C, 15min sterilizing makes enzyme deactivation simultaneously, obtains marine alga treatment solution after cooling
Or 3) doubly contain the aqueous solution of 1% mass concentration sulfuric acid by seaweed powder with its quality 20-30, be hydrolyzed 20min-30min at 121 DEG C, after cooling, obtain marine alga treatment solution;
(2) technical process of ethanol is prepared:
1) with the marine alga treatment solution fermention medium as carbon source, it consists of (NH
4)
2sO
410.8g/L, KH
2pO
45.0g/L, MgSO
47H
2o 1.1g/L, marine alga treatment solution 20g/L, 2mol/L NaOH solution or 2mol/L HCl solution adjusted to ph 4.5-5.5;
2) in the liquid of step (1) by inoculation than being 5% access Meyerozyma guilliermondii bacterial classification seed liquor, then in 30 DEG C, 150rpm cultivates 24h;
3) at 28-32 DEG C, anaerobically fermenting 5 days, not adjust ph in fermenting process, but exhaust,
4) after getting fermentation, liquid obtains ethanol by distillation.
3. according to claim 2 a kind of take marine alga as the method that bio-ethanol prepared by raw material, it is characterized in that: by inoculating than being 5% access Meyerozyma guilliermondii bacterial classification seed liquor and Saccharomyces cerevisiae bacterial classification seed liquor in the liquid of step (1), the inoculation of this both mixed strains is than being Meyerozyma guilliermondii:Saccharomyces cerevisiae=4:1.
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| WO2016118509A1 (en) * | 2015-01-20 | 2016-07-28 | Bioprocess Algae | Process and method for simultaneous saccharification and fermentation using microalgae |
| CN105272406A (en) * | 2015-10-26 | 2016-01-27 | 北京鑫鑫盛达生物工程技术有限公司 | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer |
| CN105219676A (en) * | 2015-10-26 | 2016-01-06 | 北京鑫鑫盛达生物工程技术有限公司 | A kind of cultural method of bacillus laterosporus |
| CN105254424A (en) * | 2015-10-26 | 2016-01-20 | 北京鑫鑫盛达生物工程技术有限公司 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2012045088A2 (en) * | 2010-10-01 | 2012-04-05 | The Board Of Trustees Of The University Of Illinois | Combinatorial design of highly efficient heterologous pathways |
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| WO2012045088A2 (en) * | 2010-10-01 | 2012-04-05 | The Board Of Trustees Of The University Of Illinois | Combinatorial design of highly efficient heterologous pathways |
Non-Patent Citations (5)
| Title |
|---|
| Diversity and Fermentation Products of Xylose-Utilizing Yeasts Isolated from Buffalo Feces in Thailand;WANLAPA LORLIAM等;《Microbes Environ.》;20130904;第28卷(第3期);354-360 * |
| Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples;A. W. F. Duarte等;《Extremophiles》;20131011;第17卷;1023-1035 * |
| 海藻酸转化生物乙醇研究进展;钱龙等;《中国生物工程杂志》;20130115;第33卷(第1期);摘要和第122页右栏第2段 * |
| 海藻预处理工艺研究;张雯等;《生物技术世界》;20130515(第5期);第195页2实验部分 * |
| 褐藻酸降解菌A7的发酵及产酶条件研究;褚洪蕊等;《海洋科学》;20081109;第32卷(第11期);第94页左栏2.2.1和表1 * |
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