CN1951402A - Method for preparing active polypeptide and zinc protein of sea cucumber - Google Patents
Method for preparing active polypeptide and zinc protein of sea cucumber Download PDFInfo
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- CN1951402A CN1951402A CNA200610069408XA CN200610069408A CN1951402A CN 1951402 A CN1951402 A CN 1951402A CN A200610069408X A CNA200610069408X A CN A200610069408XA CN 200610069408 A CN200610069408 A CN 200610069408A CN 1951402 A CN1951402 A CN 1951402A
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- zinc
- stichopus japonicus
- active polypeptide
- sea cucumber
- calcium
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Abstract
The invention relates to a method for producing sea cucumber active polypeptide calcium-zinc protein. Wherein, it comprises that washing the sea cucumber, breaking and grinding into sea cucumber slurry; adding distilled water, adjusting its pH value into acid; adding sodium sulfide to adjust pH value to weak acid; filtering out the deposition; adding active parenzyme into obtained sea cucumber slurry; making it uniform, adding one or two of calcium and zinc, to be aggregated into sea cucumber active polypeptide calcium-zinc protein; then adding alcohol, freezing, and ecctrically drying, to obtain the dry product. The invention can resist radiation and ultraviolet radiation; eliminate the metal toxic, etc.
Description
One, technical field
The invention belongs to sea cucumber nutrient product processing technique field, especially relate to a kind of Stichopus japonicus active polypeptide calcium, the proteic production method of zinc.
Two, background technology
The polysaccharide body protein that the Stichopus japonicus body contains is up to more than 55%, also contain trace element such as 18 seed amino acids and vitamin B1, B2, E, collagen protein, taurine and nicotinic acid, chondroitin sulfate, selenium, zinc, magnesium, germanium, Stichopus japonicus is the tonic food of rare high protein, low fat, low sugar, no cholesterol; For eating of Stichopus japonicus, great majority are to adopt traditional saline soak steaming and decocting to preserve, carry out secondary operations when edible again, because protein is dissolved in water, in the course of processing, cause the nutrition leak of Stichopus japonicus, though occurred some Stichopus japonicus deep working methods now, just adopt the method for biological extraction, extract nutrient substance in the Stichopus japonicus, instant edible, not having a kind of is rationally to add other nutrient substance at the nutritional labeling of Stichopus japonicus to process.
Three, summary of the invention
The objective of the invention is to improve the deficiency of prior art and a kind of supplement calcium be provided, improve skeletal structure, have radioprotective and ultraviolet radiation, removing in-vivo metal toxin, nutritional labeling collocation rationally, the proteic production method of the simple Stichopus japonicus active polypeptide of processing method zinc.
The object of the present invention is achieved like this, a kind of Stichopus japonicus active polypeptide calcium, the proteic production method of zinc, be characterized in that this method removes bright Stichopus japonicus intestinal, cleans, blends, wear into sea cucumber milk, in sea cucumber milk, add distilled water, adjust its pH value and be acid, add sodium sulfide then, pH value is adjusted into faintly acid, precipitate is filtered, the sea cucumber milk that filtration obtains adds active trypsin, carries out the homogenate reaction; In reacted sea cucumber milk, add one or both mixing of biological calcium or zinc, make it aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen; Freezing with adding ethanol in Stichopus japonicus active polypeptide calcium, the zinc albumen; With the frozen products centrifugal drying, obtain Stichopus japonicus active polypeptide calcium, zinc albumen dry product.
The distilled water that in sea cucumber milk, adds, both volume ratios 1: 1, adding glacial acetic acid adjustment pH value is 4.5-5.5.
Adding sodium sulfide is that the sea cucumber milk liquid agitation was added sodium sulfide after 1-3 hour, and pH value was adjusted into the 5-6 postprecipitation 1-5 hour.
The sea cucumber milk that filtration obtains adds active trypsin, and to carry out homogenate reaction be the active trypsin that adds bright Stichopus japonicus raw material weight 15-25% at sea cucumber milk, and the homogenate reaction is 6-10 hour under 40-45 ℃ of condition.
In sea cucumber milk, add biological calcium or zinc and be one or both mixing in the biological zinc of the biological calcium that adds bright Stichopus japonicus raw material weight 3-10% in the reacted sea cucumber milk of homogenate or 0.5-1%, make it aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen.
Adding ethanol in Stichopus japonicus active polypeptide calcium, the zinc albumen freezing is the ethanol that adds 3 times of its volumes in the liquid Stichopus japonicus active polypeptide calcium that will aggregate into, the zinc albumen, under-5 ℃ to-15 ℃ conditions freezing 8-10 hour.
Centrifugal drying is the traditional vacuum drying under 5-40 ℃ of condition.
The active trypsin that adds can be a kind of or any two or more mixing in pancreas enzyme powder, pancreatin protein liquid, the pancreas catabolic enzyme.
Compared with the prior art the present invention has following distinguishing feature and good effect: the present invention adopts and add biological calcium in sea cucumber milk, zinc element, aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen, Stichopus japonicus active polypeptide calcium, zinc albumen has wide biological activity, Stichopus japonicus active polypeptide calcium protein can replenish calcareous that human body runs off, improve skeletal structure, Stichopus japonicus active polypeptide zinc albumen has radioprotective and ultraviolet radiation, remove heavy metal of body and free radical, effects such as detoxifcation, to digestive tract ulcer, diseases such as myocardial infarction have certain therapeutical effect, effect with beauty and skin care, can also alleviate smoking and environmental pollution harm to human body, allergy had certain curative effect, particularly obvious especially to the seizure of heavy metals such as lead, have the health-care effect of broad-spectrum high efficacy and high medical value; The present invention adds sodium sulfide in process of production, and pH value is adjusted into the 5-6 postprecipitation, the heavy metal lead in the sea cucumber milk, copper etc. can be filtered out; Add active trypsin, purpose is that the Stichopus japonicus peptide is cracked into several sections, becomes ionic condition, is convenient to polymerization and absorbs; Traditional vacuum drying under 40 ℃ of following conditions does not make product lose activity.
Four, specific embodiments
Embodiment 1, and a kind of Stichopus japonicus active polypeptide calcium, the proteic production method of zinc are that the work Stichopus japonicus is removed intestinal, cleans, blends, and wear into sea cucumber milk; The distilled water that adds one times of sea cucumber milk volume adds glacial acetic acid, and adjusting its pH value is 4.5-5.5; To adjust the sea cucumber milk liquid agitation and add sodium sulfide after 1-3 hour, pH value will be adjusted into the 5-6 postprecipitation 1-5 hour; Filter, precipitate is filtered collect reason elsewhere, filter the trypsin that the sea cucumber milk that obtains adds bright Stichopus japonicus weight 20%, the homogenate reaction is 6-10 hour under 40-45 ℃ of condition; One or both mixing in reacted sea cucumber milk in the biological zinc of the calcium of the bright Stichopus japonicus weight 3-6% of adding or 0.5-1% make it aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen; Synthetic Stichopus japonicus active polypeptide calcium, zinc albumen are added the ethanol of 3 times of its volumes, under-5 ℃ to-15 ℃ conditions freezing 8-10 hour; With frozen products traditional vacuum drying under 40 ℃ of following conditions, obtain Stichopus japonicus active polypeptide calcium, zinc albumen dry product, specifically bright Stichopus japonicus 500 grams that adopt after cleaning in the present embodiment, obtain 480 milliliters of sea cucumber milks behind the defibrination, add 480 ml waters, adding glacial acetic acid adjustment pH value is 4.5, to adjust the sea cucumber milk liquid agitation and add sodium sulfide after 1 hour, pH value is adjusted into 5 postprecipitations to be filtered in 1 hour, the sea cucumber milk that filtration obtains adds 75 gram pancreas enzyme powders, the homogenate reaction is 6 hours under 40 ℃ of conditions, add 15 gram biological calciums and 2.5 gram biological zinc again, make it aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen, with synthetic Stichopus japonicus active polypeptide calcium, zinc albumen adds the ethanol of 3 times of its volumes, under-5 ℃ of conditions freezing 10 hours,, obtain Stichopus japonicus active polypeptide calcium with frozen products traditional vacuum drying under 40 ℃ of conditions, zinc albumen dry product.
Embodiment 2, a kind of Stichopus japonicus active polypeptide calcium, the proteic production method of zinc, specifically bright Stichopus japonicus 1000 grams that adopt after cleaning in the present embodiment, obtain 950 milliliters of sea cucumber milks behind the defibrination, add 950 ml waters, adding glacial acetic acid adjustment pH value is 5.5, to adjust the sea cucumber milk liquid agitation and add sodium sulfide after 3 hours, pH value is adjusted into 5 postprecipitations to be filtered in 5 hours, the sea cucumber milk that filtration obtains adds 250 gram pancreatin protein liquids, the homogenate reaction is 10 hours under 45 ℃ of conditions, adds 100 gram biological calciums again, makes it aggregate into Stichopus japonicus active polypeptide calcium protein, the ethanol that synthetic Stichopus japonicus active polypeptide calcium protein is added 3 times of its volumes, under-15 ℃ of conditions freezing 8 hours,, obtain Stichopus japonicus active polypeptide calcium protein dry product with frozen products traditional vacuum drying under 35 ℃ of conditions.
Embodiment 3, a kind of Stichopus japonicus active polypeptide calcium, the proteic production method of zinc, specifically bright Stichopus japonicus 2000 grams that adopt after cleaning in the present embodiment, obtain 1980 milliliters of sea cucumber milks behind the defibrination, add 1980 ml waters, adding glacial acetic acid adjustment pH value is 4.5, to adjust the sea cucumber milk liquid agitation and add sodium sulfide after 2 hours, pH value is adjusted into 5 postprecipitations to be filtered in 3 hours, the sea cucumber milk that filtration obtains adds 400 gram pancreas enzyme powders, the pancreatin protein liquid, the mixed enzyme of pancreas catabolic enzyme, the homogenate reaction is 8 hours under 43 ℃ of conditions, adds 16 gram biological zinc again, makes it aggregate into Stichopus japonicus active polypeptide zinc albumen, the ethanol that synthetic Stichopus japonicus active polypeptide zinc albumen is added 3 times of its volumes, under-10 ℃ of conditions freezing 9 hours,, obtain Stichopus japonicus active polypeptide zinc albumen dry product with frozen products traditional vacuum drying under 5 ℃ of conditions.
Claims (8)
1, a kind of Stichopus japonicus active polypeptide calcium, the proteic production method of zinc, it is characterized in that this method removes bright Stichopus japonicus intestinal, cleans, blends, wear into sea cucumber milk, in sea cucumber milk, add distilled water, adjust its pH value and be acid, add sodium sulfide then, pH value is adjusted into faintly acid, precipitate is filtered, filter the sea cucumber milk that obtains and add active trypsin, carry out the homogenate reaction; In reacted sea cucumber milk, add one or both mixing of biological calcium or zinc, make it aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen; Freezing with adding ethanol in Stichopus japonicus active polypeptide calcium, the zinc albumen; With the frozen products centrifugal drying, obtain Stichopus japonicus active polypeptide calcium, zinc albumen dry product.
2, a kind of Stichopus japonicus active polypeptide calcium according to claim 1, the proteic production method of zinc is characterized in that the distilled water that adds in sea cucumber milk, both volume ratios 1: 1, and adding glacial acetic acid, to adjust pH value be 4.5-5.5.
3, a kind of Stichopus japonicus active polypeptide calcium according to claim 1, the proteic production method of zinc, it is characterized in that adding sodium sulfide is that the sea cucumber milk liquid agitation was added sodium sulfide after 1-3 hour, and pH value was adjusted into the 5-6 postprecipitation 1-5 hour.
4, a kind of Stichopus japonicus active polypeptide calcium according to claim 1, the proteic production method of zinc, it is characterized in that filtering the sea cucumber milk that obtains and add active trypsin to carry out homogenate reaction be the active trypsin that adds bright Stichopus japonicus raw material weight 15-25% at sea cucumber milk, the homogenate reaction is 6-10 hour under 40-45 ℃ of condition.
5, a kind of Stichopus japonicus active polypeptide calcium according to claim 1, the proteic production method of zinc, it is characterized in that in sea cucumber milk adding biological calcium or zinc and be one or both mixing in the biological zinc of the biological calcium that adds bright Stichopus japonicus raw material weight 3-10% in the reacted sea cucumber milk of homogenate or 0.5-1%, make it aggregate into Stichopus japonicus active polypeptide calcium, zinc albumen.
6, a kind of Stichopus japonicus active polypeptide calcium according to claim 1, the proteic production method of zinc, it is characterized in that adding in Stichopus japonicus active polypeptide calcium, the zinc albumen ethanol freezing is the ethanol that adds 3 times of its volumes in the liquid Stichopus japonicus active polypeptide calcium that will aggregate into, the zinc albumen, under-5 ℃ to-15 ℃ conditions freezing 8-10 hour.
7, the proteic production method of a kind of Stichopus japonicus active polypeptide zinc according to claim 1 is characterized in that centrifugal drying traditional vacuum drying under 5-40 ℃ of condition.
8,, it is characterized in that the active trypsin that adds can be a kind of or any two or more mixing in pancreas enzyme powder, pancreatin protein liquid, the pancreas catabolic enzyme according to claim 1 or the proteic production method of 4 described a kind of Stichopus japonicus active polypeptide zinc.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200610069408A CN1951402B (en) | 2006-10-13 | 2006-10-13 | Method for preparing active polypeptide and zinc protein of sea cucumber |
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| CN200610069408A CN1951402B (en) | 2006-10-13 | 2006-10-13 | Method for preparing active polypeptide and zinc protein of sea cucumber |
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| CN1951402A true CN1951402A (en) | 2007-04-25 |
| CN1951402B CN1951402B (en) | 2010-05-12 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156947B (en) * | 2007-09-20 | 2011-01-19 | 黄世宽 | Ginseng source peptide albumen, its preparing method and application |
| CN102578589A (en) * | 2012-03-08 | 2012-07-18 | 宁波大学 | Preparation method and application of sea cucumber polypeptide chelated active calcium |
| CN104013066A (en) * | 2014-05-22 | 2014-09-03 | 青岛金谷原生命科技有限公司 | Preparing method of sea cucumber polypeptide granules |
| CN109355340A (en) * | 2018-11-23 | 2019-02-19 | 胜田(福清)食品有限公司 | A kind of preparation method with high thermal stability sea cucumber antioxidation chelation peptide |
| CN114522183A (en) * | 2022-01-24 | 2022-05-24 | 江南大学 | Sea cucumber extract for increasing bone mineral density |
-
2006
- 2006-10-13 CN CN200610069408A patent/CN1951402B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156947B (en) * | 2007-09-20 | 2011-01-19 | 黄世宽 | Ginseng source peptide albumen, its preparing method and application |
| CN102578589A (en) * | 2012-03-08 | 2012-07-18 | 宁波大学 | Preparation method and application of sea cucumber polypeptide chelated active calcium |
| CN104013066A (en) * | 2014-05-22 | 2014-09-03 | 青岛金谷原生命科技有限公司 | Preparing method of sea cucumber polypeptide granules |
| CN104013066B (en) * | 2014-05-22 | 2015-06-03 | 青岛金谷原生命科技有限公司 | Preparing method of sea cucumber polypeptide granules |
| CN109355340A (en) * | 2018-11-23 | 2019-02-19 | 胜田(福清)食品有限公司 | A kind of preparation method with high thermal stability sea cucumber antioxidation chelation peptide |
| CN114522183A (en) * | 2022-01-24 | 2022-05-24 | 江南大学 | Sea cucumber extract for increasing bone mineral density |
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| CN1951402B (en) | 2010-05-12 |
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