CN101156947B - Ginseng source peptide albumen, its preparing method and application - Google Patents
Ginseng source peptide albumen, its preparing method and application Download PDFInfo
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- CN101156947B CN101156947B CN2007101522526A CN200710152252A CN101156947B CN 101156947 B CN101156947 B CN 101156947B CN 2007101522526 A CN2007101522526 A CN 2007101522526A CN 200710152252 A CN200710152252 A CN 200710152252A CN 101156947 B CN101156947 B CN 101156947B
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Abstract
The invention discloses a ginseng source peptide protein healthy product which has the functions of calcium supplement, free radical and heavy metal removal, radiation hardening, anti-ultraviolet radiation, immunity improvement and detoxification, and the preparation method thereof. The healthy product of the invention is made of raw materials according to the following percent by weight: 300 to 600 parts of sea cucumbers in which intestines and viscera are removed, 30 to 60 parts of the mixtures of rabbit liver and kidney which are induced through compound protein zinc, 30 to 60 parts of parenzyme, 5 to 20 parts of compound protein zinc, and 6 to 28 parts of bio-calcium. The healthy product of the invention has the advantages that the processing method is simple, the nutrient content is comprehensive, the efficiency among each component is synergistic and complementary, and the healthy product has the efficiencies of calcium supplement, skeleton structure improvement, free radical and heavy metal removal, radiation hardening, anti-ultraviolet radiation, immunity improvement and body condition enhancement and other multiple health protection efficiencies.
Description
Technical field
The present invention relates to a kind of health-care products, relate in particular to a kind of animal proteinum goods that utilize materials such as Stichopus japonicus, Hepar Leporis seu Oryctolagi, rabbit kidney, peptide, calcium, protein-zinc to be prepared from multiple health cares such as replenishing the calcium, remove free radical, raising immunity, the invention still further relates to the preparation method of this health-care products, belong to the animal proteinum manufacture field.
Background technology
GL-PP also contains 18 seed amino acids and vitamin B1, B2, E, collagen protein, taurine and nicotinic acid chondroitin sulfate, all multiple trace elements such as selenium, zinc, magnesium, germanium up to more than 55% in the Stichopus japonicus body.Stichopus japonicus belongs to a rare class high protein, low fat, low sugar, the nutriment of no cholesterol, for eating of Stichopus japonicus, mostly adopt the steaming and decocting of heating of traditional saline to preserve, the other worker of secondary again when edible, because protein is water-soluble, in the course of processing, cause a large amount of losses of protein and other nutritional labeling easily, begin to have sold nutrient substance now on the market from instant edible in the Stichopus japonicus, for extremely, still there is not a kind of nutrition rationally to add other nutrient substance and prepare health-care products so far at Stichopus japonicus.
Summary of the invention
The present invention's technical problem at first to be solved is to overcome the deficiencies in the prior art, a kind of health product (ginseng source peptide albumen) of replenishing the calcium, removing free radical, improve multiple health care such as immunity that have are provided, these health product mainly are raw material with the Stichopus japonicus and have added a certain proportion of other animal proteinum, have and replenish the calcium, zinc supplement improves skeletal structure, have radioprotective and remove interior free yl, improve a series of health-care effecies such as body immunity.
The present invention's technical problem at first to be solved is achieved through the following technical solutions:
A kind of have health product of replenishing the calcium, remove functions such as free radical and heavy metal, radioprotective and ultraviolet radiation, raising immunity, detoxifcation, mainly made by the raw material of following weight portion:
Remove Stichopus japonicus 300-600 part of intestinal and internal organs, mixture 30-60 part of Hepar Leporis seu Oryctolagi after Multiprotein-zinc is induced and rabbit kidney, trypsin 30-60 part, Multiprotein-zinc 5-20 part and biological calcium 6-28 part;
Preferably, the weight portion of each raw material is:
Remove Stichopus japonicus 450-550 part of intestinal and internal organs, mixture 40-55 part of Hepar Leporis seu Oryctolagi after Multiprotein-zinc is induced and rabbit kidney, trypsin 45-55 part, Multiprotein-zinc 8-15 part, biological calcium 10-15 part;
Preferred, the weight portion of each raw material is:
Remove 500 parts of the Stichopus japonicuss of intestinal and internal organs, 50 parts in the Hepar Leporis seu Oryctolagi after Multiprotein-zinc is induced and the mixture of rabbit kidney, 50 parts of trypsin, 10 parts of Multiprotein-zincs, 12 parts of biological calciums.
The Hepar Leporis seu Oryctolagi after described Multiprotein-zinc is induced and the mixture of rabbit kidney prepare in accordance with the following methods: commercially available 0.2mg Multiprotein-zinc is injected the abdominal part of rabbit by hypodermic mode, induced for 2 weeks after, take out liver and the kidney of rabbit, promptly.
Multiprotein-zinc: the main mixture of forming by collagen protein and zinc etc.; Multiprotein-zinc also contains iodine, ferrum, copper, manganese, chromium, cobalt, calcium and magnesium biological element except containing biologic trace element zinc, selenium, these teleorganic elements all are that the form with protein complex exists.Also contain 18 seed amino acids in addition, comprising 8 seed amino acids of needed by human.Also contain the lecithin very useful to human brain.(Multiprotein-zinc can be bought by commercial sources and obtain, for example: the technology development co. of high and new technology industrial development zone, Yantai: Jining Yi Shengtang bio tech ltd, the development zone, city is appointed in Jining, Shandong; Its trade name is " Multiprotein-zinc ")
Trypsin selectively in the aminosal by peptide chain that lysine or arginic carboxyl constituted, can digestion dissolve degeneration egg matter, unmodified protein there is not effect, therefore, can make decomposition, thinning such as pus, sputum, blood clot, be easy to drain and get rid of, quicken wound surface and purify, promote granulation tissue new life, also have anti-inflammatory effect in addition.
Biological calcium (also claiming biological calcium carbonate) is that calcium content height, its calcium content are 3 times of calcium lactate by the refining high-quality calcium source that extracts in the biological raw materials such as Concha Ostreae, 4 times of calcium gluconate; Easily absorption, free from extraneous odour, biological value height, side effect is little.Biological calcium can obtain in the market purchase, for example: and Zhengzhou Ruipu Bioengineering Co., Ltd.'s (high-tech industrial development zone, Zhengzhou, Henan Province is auspicious to reach the road No. 96), or Zhengzhou Fu Run moral biological engineering company limited etc.Its quality standard is as follows: calcium carbonate (in butt) %: 〉=97.6, loss on drying % :≤1.0, hydrochloric acid insoluble substance % :≤0.30, barium (Ba) % :≤0.03, heavy metal contains (Pb meter) % :≤0.001, arsenic (As) % :≤0.0003, cadmium (Cd) % :≤0.00002, fineness (order):＞180.
Used various raw materials all can be bought from market and obtain among the present invention, and its specification is food stage or pharmaceutical grade.
Another technical problem to be solved by this invention provides a kind of method for preparing above-mentioned health product.
Another technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of method for preparing above-mentioned health product comprises:
(1) takes by weighing each raw material by following weight portion: remove Stichopus japonicus 300-600 part of intestinal and internal organs, mixture 30-60 part of Hepar Leporis seu Oryctolagi after Multiprotein-zinc is induced and rabbit kidney, trypsin 30-60 part, Multiprotein-zinc 5-20 part and biological calcium 6-28 part;
(2) Hepar Leporis seu Oryctolagi after protein-zinc is induced and the mixture of rabbit kidney mix with the Stichopus japonicus of removing intestinal and internal organs, are dissolved in the water after grinding, and add islets of langerhans protease, regulate pH value to 8-9, and reaction is 4-6 hour under 40 ℃-50 ℃ temperature; After reaction is finished, in reactant liquor, add biological calcium and remaining protein-zinc, regulate pH value to 7, stir, be warmed up to 40 ℃ of reactions 6 hours, get mixture;
(3) in mixture, add 98% above ethanol, under-10 ℃ to-20 ℃ temperature, carry out freezing polymerization; Centrifugal, abandoning supernatant is with the solid vacuum drying, promptly.
Wherein, the mixture of described Hepar Leporis seu Oryctolagi and rabbit kidney is that Hepar Leporis seu Oryctolagi and rabbit kidney are mixed and get according to arbitrary proportion.
In the said method, in order to reach better effect:
In the step (3), in mixture, preferably be incorporated as 3 times of volume of ethanol of mixture; Under-10 ℃ to-20 ℃ temperature, carry out freezing polymerization reaction 6-8 hour, more preferably carry out freezing polymerization reaction 6 hours; Described vacuum drying preferably carries out vacuum drying under 5-40 ℃ of condition.
Health product of the present invention can replenish the calcium that human body runs off, also can improve skeletal structure, radioprotective and ultraviolet radiation are removed interior free yl and heavy metal, detoxifcation, multiple health care such as human body immunity improving ability, to digestive tract ulcer, myocardial infarction, hepatitis, alcoholic liver, fatty liver, prostate have the obvious treatment effect.Health product of the present invention also have the effect of cosmetology, can alleviate smoking and environmental pollution to the harm that human body caused, and various anaphylactic diseases are also had certain curative effect, have certain medical value.
The usage of health-care products of the present invention and consumption: oral: each 400mg, every day three times.
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall within the scope of protection of the present invention the details of technical solution of the present invention and form.
The Hepar Leporis seu Oryctolagi after Multiprotein-zinc is induced and the mixture of rabbit kidney prepare in accordance with the following methods: with commercially available 0.2mg Multiprotein-zinc (available from Jining Yi Shengtang bio tech ltd, its trade name is " Multiprotein-zinc ") inject the abdominal part of rabbit by hypodermic mode, after inducing for 2 weeks, take out liver and the kidney of rabbit, promptly.
Embodiment 1
Go intestinal to clean 1000 grams Stichopus japonicus alive, get clean Stichopus japonicus 500 grams, add 50 gram Multiprotein-zincs after inducing Hepar Leporis seu Oryctolagi and the mixture of rabbit kidney, add the water mill slurry, add 50 gram trypsin again and heat 40 ℃-50 ℃, transferring pH is 8-9 reaction 6 hours, after becoming liquid, add Multiprotein-zinc (Jining Yi Shengtang bio tech ltd, its trade name are " Multiprotein-zincs ") 10 grams, biological calcium (Zhengzhou Ruipu Bioengineering Co., Ltd., its trade name is " biological calcium ") 12 grams, transferring pH is 7, adds 1500 milliliters of ethanol of 3 times of volumes, reacted freezing polymerizations 6 hours at-10 ℃ to-20 ℃, centrifugal, abandoning supernatant will be deposited in vacuum drying under the 5-40 ℃ of condition, after the drying, promptly.
Embodiment 2
Go intestinal to clean 2000 grams Stichopus japonicus alive, get clean Stichopus japonicus 1050 grams, add 100 gram Multiprotein-zincs after inducing Hepar Leporis seu Oryctolagi and the mixture of rabbit kidney, add the water mill slurry, add 100 gram trypsin again and heat 40 ℃-50 ℃, transferring pH is 8 reactions 8 hours, behind the one-tenth liquid, add Multiprotein-zinc (Jining Yi Shengtang bio tech ltd, its trade name is " Multiprotein-zinc ") 20 grams, biological calcium (Zhengzhou Ruipu Bioengineering Co., Ltd., its trade name is " biological calcium ") 25 grams, transferring pH is 7, stir, be warmed up to 40 ℃ of reactions 6 hours, add 300 milliliters of ethanol, reacted freezing polymerizations 6 hours at-10 ℃ to-20 ℃, centrifugal, abandoning supernatant will be deposited in vacuum drying under the 5-40 ℃ of condition, after the drying, promptly.
Embodiment 3
Go intestinal to clean 600 grams Stichopus japonicus alive, get clean Stichopus japonicus 300 grams, add 30 gram Multiprotein-zincs after inducing Hepar Leporis seu Oryctolagi and the mixture of rabbit kidney, add the water mill slurry, add 40 gram trypsin again and heat 40 ℃-50 ℃, transferring pH is 8 reactions 8 hours, behind the one-tenth liquid, add Multiprotein-zinc (Jining Yi Shengtang bio tech ltd, its trade name is " Multiprotein-zinc ") 20 grams, biological calcium (Zhengzhou Ruipu Bioengineering Co., Ltd., its trade name is " biological calcium ") 6 grams, transferring pH is 7, stir, be warmed up to 40 ℃ of reactions 6 hours, add 200 milliliters of ethanol, reacted freezing polymerizations 6 hours at-10 ℃ to-20 ℃, centrifugal, abandoning supernatant will be deposited in vacuum drying under the 5-40 ℃ of condition, after the drying, promptly.
Embodiment 4
Go intestinal to clean 1200 grams Stichopus japonicus alive, get clean Stichopus japonicus 600 grams, add 60 gram Multiprotein-zincs after inducing Hepar Leporis seu Oryctolagi and the mixture of rabbit kidney, add the water mill slurry, add 40 gram trypsin again and heat 40 ℃-50 ℃, transferring pH is 8 reactions 8 hours, behind the one-tenth liquid, add Multiprotein-zinc (Jining Yi Shengtang bio tech ltd, its trade name is " Multiprotein-zinc ") 20 grams, biological calcium (Zhengzhou Ruipu Bioengineering Co., Ltd., its trade name is " biological calcium ") 28 grams, transferring pH is 7, stir, be warmed up to 40 ℃ of reactions 6 hours, add 300 milliliters of ethanol, reacted freezing polymerizations 6 hours at-10 ℃ to-20 ℃, centrifugal, abandoning supernatant will be deposited in vacuum drying under the 5-40 ℃ of condition, after the drying, promptly.
The efficacy test of replenishing the calcium of test example 1 health-care products of the present invention
Excision is tried the female rats bilateral ovaries, and to cause osteoporosis rat be model (be divided into feeding and not feeding group), with femur bending strength (I), content of mineral substances (femur ash) (II) and uterus weight (II) be observation index, excision ovary feeding health-care products of the present invention (embodiment of the invention is prepared) after 8 days, continuous irrigation stomach feeding 15 days, the result compares administration group (above-mentioned three kinds of dosage) and index I, II, the III of the matched group of administration all do not have significant difference, the P value of three dosage groups is respectively 0.001,0.01, demonstrate dose-effect relationship with 0.05.Histological examination, feeding group sclerotin thickens, the trabecular bone showed increased.
The 2 health-care products immunoregulation effect tests of the present invention of test example
The 18-22g Male Kunming strain mice is divided into 4 groups at random, i.e. three experimental grouies and a distilled water matched group, health-care products of the present invention (embodiment of the invention is prepared) is given the mice feeding of three experimental grouies according to high, medium and low three dosage, irritate stomach every day once, choosing is done Turnover of Mouse Peritoneal Macrophages and is engulfed chicken red blood cell test (half intracorporal method, DNFB inducing mouse DTH test (ear swelling method) serum hemolysin is measured (blood clotting degree) after continuous 30 days.
Result of the test: laboratory animal body weight and weight increase are heavy, thymus/body weight, spleen/body weight there is not obvious influence, the ability that Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell that strengthens is arranged, can promote the tardy paraphilia reaction of dinitrofluorobenzene (DNFB) inducing mouse (DTH) to form, can improve mice hemagglutination degree, according to 3.1.3 item in the Ministry of Public Health health food function assessment assessment process and the method for inspection, judge that health-care products of the present invention has the nonspecific immunity of enhancing and cellular immunization and humoral immune function.
The health-care effect viewing test of test example 3 health-care products of the present invention
One, the physical data of the object of observation
Observe 56 routine patients altogether.Man's 32 examples, women 24 examples, 52 years old mean age.56 patients are because physical function is low, and immunity is lower, and leukocytic par all is lower than 4000/cubic millimeter in the body.
Two, instructions of taking
Eat health-care products of the present invention (embodiment 1 is prepared) 3 every day, and each 400mg took 15 days continuously.
Three, model case
1, certain man, 72 years old age; Because old and feeble, immunologic hypofunction is in particular in that leukocyte count is 2700/mm
3, be lower than minimum healthy amount 4000/mm
3, so inappetence, lassitude and weak, easy catching a cold, hospitalization also takes a turn for the better.Take health-care products of the present invention, survey leukocyte after six days two of every days, rise to 5800/mm
3, appetite takes a turn for the better, seldom flu.
2, certain man, at 54 years old age, leukocyte is for a long time 3000 more, and muscle power, sleep all do not feel well, and take health-care products of the present invention, and after 15 days, leukocyte rises to 6000/mm
3More than, the mental status sensation also takes a favorable turn than before taking.
Four, observed result
56 observed routine white blood cell count are lower than the normal value patient, through eating health-care products of the present invention continuously after 15 days, white blood cell count all returns to more than 4000/cubic millimeter in its body, and patient's immunity is significantly increased, and the patient's body function and the mental status all take an evident turn for the better.
Claims (6)
1. ginseng source peptide albumen health product that have replenishing the calcium, improve immunity function, it is characterized in that, mainly make: Stichopus japonicus 300-600 part by the raw material of following weight portion, hepatic and/or renal mixture 30-60 part of rabbit after Multiprotein-zinc is induced, trypsin 30-60 part, Multiprotein-zinc 5-20 part and biological calcium 6-28 part; Described Stichopus japonicus is removed the internal organs that comprise intestinal; The hepatic and/or renal mixture of rabbit after described Multiprotein-zinc is induced prepares in accordance with the following methods: the 0.2mg Multiprotein-zinc is injected the abdominal part of rabbit by hypodermic mode, induced for 2 weeks after, take out liver and the kidney of rabbit, promptly;
Its preparation method comprises:
(1) takes by weighing each raw material by described weight portion;
(2) the hepatic and/or renal mixture of the rabbit after Multiprotein-zinc is induced mixes with the Stichopus japonicus of removing the internal organs that comprise intestinal, is dissolved in the water after grinding, and adds trypsin, regulates pH value to 8-9, and reaction is 4-6 hour under 40 ℃-50 ℃ temperature; After reaction is finished, in reactant liquor, add biological calcium and remaining protein-zinc, regulate pH value to 7, stir, be warmed up to 40 ℃ of reactions 6 hours, get mixture;
(3) in mixture, add ethanol, under-10 ℃ to-20 ℃ temperature, carry out freezing polymerization; Centrifugal, abandoning supernatant is with the solid vacuum drying, promptly.
2. according to the ginseng source peptide albumen health product of claim 1, it is characterized in that the weight portion of each raw material is: Stichopus japonicus 450-550 part, hepatic and/or renal mixture 40-55 part of rabbit after Multiprotein-zinc is induced, trypsin 45-55 part, Multiprotein-zinc 8-15 part, biological calcium 10-15 part.
3. according to the ginseng source peptide albumen health product of claim 1, it is characterized in that the weight portion of each raw material is: 500 parts of Stichopus japonicuss, 50 parts in the hepatic and/or renal mixture of the rabbit after Multiprotein-zinc is induced, 50 parts of trypsin, 10 parts of Multiprotein-zincs, 12 parts of biological calciums.
4. according to the ginseng source peptide albumen health product of claim 1, it is characterized in that: in mixture, be incorporated as the ethanol more than 98% concentration of 3 times of volumes of mixture in the step (3).
5. according to the ginseng source peptide albumen health product of claim 1, it is characterized in that: under-10 ℃ to-20 ℃ temperature, carry out freezing polymerization reaction 6-8 hour after in mixture, adding 98% above ethanol in the step (3).
6. according to the ginseng source peptide albumen health product of claim 1, it is characterized in that: the vacuum drying described in the step (3) is to carry out vacuum drying under 5-40 ℃ temperature conditions.
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| CN2007101522526A CN101156947B (en) | 2007-09-20 | 2007-09-20 | Ginseng source peptide albumen, its preparing method and application |
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| CN2007101522526A CN101156947B (en) | 2007-09-20 | 2007-09-20 | Ginseng source peptide albumen, its preparing method and application |
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| CN101156947A CN101156947A (en) | 2008-04-09 |
| CN101156947B true CN101156947B (en) | 2011-01-19 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1211423A (en) * | 1998-05-04 | 1999-03-24 | 大连生物化学制药厂 | Fe-Ca nutrient combination and its preparation method |
| CN1494914A (en) * | 2002-08-29 | 2004-05-12 | 宋述孝 | Prduction method of holothurian oral lozenge |
| CN1951402A (en) * | 2006-10-13 | 2007-04-25 | 黄世宽 | Method for preparing active polypeptide and zinc protein of sea cucumber |
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- 2007-09-20 CN CN2007101522526A patent/CN101156947B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1211423A (en) * | 1998-05-04 | 1999-03-24 | 大连生物化学制药厂 | Fe-Ca nutrient combination and its preparation method |
| CN1494914A (en) * | 2002-08-29 | 2004-05-12 | 宋述孝 | Prduction method of holothurian oral lozenge |
| CN1951402A (en) * | 2006-10-13 | 2007-04-25 | 黄世宽 | Method for preparing active polypeptide and zinc protein of sea cucumber |
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