CN1951209A - Method for improving milk lactoprotein content by use of processing protein feedstuff - Google Patents

Abstract

The invention relates to a method for treating protein forage to improve the milk protein content. Wherein, it adds polyhydroxy ketone or polyhydroxy aldehyde into protein material, heating into cud protective protein; the invention can improve the protein cud through rate for 30-70%, reduce the protein waste, and improve the protein entering into small intestine for 20-40%, to improve the milk protein content. The invention can improve the forage utilization and reduce pollution.

Description

A kind of by handling the method that protein feed improves the milk content of milk protein

[technical field]

The present invention relates to a kind ofly, belong to the field of herding subject Animal nutrition and forage science by handling the method that protein feed improves the milk content of milk protein.The invention still further relates to and handle used protectant kind of protein feed and adding proportion, and technical parameters such as the heating-up temperature in the processing technology of this ruminally-protected protein feed, heat time heating time; Also relate to the protein feeds handled with this method application technology and method ruminants such as milk cow, milch goats.

[background technology]

1. reduce the meaning of protein feeds cud degradation rate

Ruminant produces in our times animal husbandry is produced and occupies critical role, and countries in the world generally are subjected to protein feed resource restriction in short supply in the development ruminant produces, also exist ruminant to nitrogenous level of resources utilization problem on the low side simultaneously.In recent years, along with the mankind propose higher requirement to livestock products quality and environmental protection, the animal producer is faced with the multiple problem that improves efficiency of feed utilization and improve the livestock products quality and reduce ammonia nitrogen discharge capacity in the animal fecaluria.Limiting amino acid and compositional model thereof are the key factors of nitrogen substance utilization ratio in the decision animal body.Therefore, make full use of ruminant proteinaceous nutrient biological characteristic, reduce protein feeds consumption in the daily ration by regulation and control to the limiting amino acid balance, improve the utilization ratio of animal to feed protein to greatest extent, the minimizing nitrogenous source is an important channel of saving protein feeds with the discharge quantity of excrement, urine.This also is the focus and the focus of ruminant proteinaceous nutrient research in recent years.It is to improving China's plant-eating animal level of production and keeping its sustainable development significant.

Along with the continuous adjustment of culturing structure, the raising scale of China milk cow and beef cattle is raised scattered to the plant layout breed by peasant household gradually and is gone through transition.Because the restriction of feeding and management level and germ plasm resource, the production performance of China milk cow and beef cattle generally are lower than external same period of level.Wherein, with regard to the feeding and management level, the proteinaceous nutrient of daily ration has limited the production performance performance of milk cow and beef cattle to a great extent.Domestic and international research shows, the production performance production performance of ruminant and children's age and young ruminant preferably is subjected to the rumen bypass protein contents level restriction in the daily ration the most obvious.

2. reduce the necessity of good protein feed cud percent of pass

Byproduct after the oil plants seed oil expression is the main protein feed resource of domestic animal such as cattle and sheep, and their ratio in concentrated feed is at 10%-40%.Ruminant has unique digestion structure, has huge ruminal digestion system, the main nutrient matter of feed many at cud by microorganism catabolism and synthetic thalline, this helps cattle and sheep to the digesting and assimilating of roughage, but has also reduced the utilization rate of high-quality feed.Test shows high-quality protein feed such as the soybean dregs of rice in cud 24 hours, and the degradation rate of protein reaches (Diao Qiyu, 2004) more than 60%.The degradation rate of protein feed is too high can to cause protein degradation and the synthetic imbalance of microprotein, causes the unnecessary loss of nitrogen in the cud.Normally whole cud protein degradation surplus.This is because after the quick degraded of protein, NH in the cud ₃Concentration reaches maximum rapidly, and microbial growth speed and protein synthesis rate can not increase simultaneously, and after degrading fast, can and then occur the situation of feed nitrogen hunger in the cud usually.

For high yield cow, the content that only depends on the synthetic mycoprotein of rumen microorganism not satisfy lactoprotein in the needs, particularly milk of its production is affected, if the by-pass protein in the feed is not enough, directly influences the protein content in suckling.Many studies show that; improve good protein feed cud percent of pass and can improve the production performance of ruminant; their quantity that improves non-protein degradation that studies show that can improve the production performance of animal, and the amino acid that non-protein degradation provided can replenish behind the protein protection because the amino acid of that part of microbial bacteria body protein that the lumen fermentation deficiency is lost.Most of experimental studies show; the discharge of rumen microorganism albumen of the high daily ration of rumen-protected protein content of feeding obviously reduces; the forage protein amount that enters small intestine can obviously increase, and total protein content of outflow is that microbial cells albumen flow and forage protein flow sum are unaffected.Avoid good protein feed degradation speed in cud too fast by suitable guard method, can increase the quantity of rumen bypass protein, improve the utilization rate of nitrogen; increase the deposition (State of Zhao's fine jade, 2003) of nitrogen, increase productivity; improve the utilization rate of protein feeds, reduce environmental pollution.

3. study the theoretical foundation and the test method of rumen bypass protein

The research of animal protein Nutrition and Metabolism is noticeable very active again scientific research field in the world wide always, and from 1816, it was notion (Munro, 1964 of essential nutrients in the animal daily ration that Magendie proposes nitrogen substance; Marliss, 1977), Germany scientist Zuntz in 1891 and Hagemann find that ruminant tumor gastric can utilize since the nonprotein nitrogen (NPN), and the various countries scientist has produced very big interest to ruminal metabolism, has promoted people's deepening continuously to ruminant protein digestibility and metabolism holistic approach.At present, fully aware of to the approach of digestion of ruminant tumor gastric internal protein and metabolism.

Owing to a large amount of microorganisms are arranged in the cud, comprise bacterium, protozoon and fungi, ruminant is to the digestion of feed protein and utilize approach obviously to be different from nonruminant.It is generally acknowledged that after feed protein entered cud, a part was degraded by microorganisms and is oligopeptides, amino acid and ammonia; Rumen microorganism utilizes the volatile fatty acid that fermentation generates etc. to make carbon skeleton again, utilizes energy that lumen fermentation discharges with this part oligopeptides, amino acid and ammonia synthesized micro-organism bacterium protein (MCP) again.Along with chyme enters abomasum and small intestine, the digestive juice of being secreted by animal (enzyme) resolves into little peptide or free amino acid and absorbs for body these microproteins again together with non-protein degradation UDP in the feed (comprising the little peptide of cud intact proteins and part).

In ruminant proteinaceous nutrient research field,, make the research of ruminant proteinaceous nutrient that leapfrog development arranged along with the utilization of fistula technology and the development and the application of chyme circulation mensuration and isotope technology.Protein feeds is had very deep research at the cud degradation rate with on enteral measure of digestibility method both at home and abroad.Method commonly used at present has intracorporal method, half intracorporal method (Nylon Bag method), in-vitro simulated method, animal model method etc.These evaluation methods have been set up the operation sequence of standard basically, but each laboratory and inspection center use for reference mutually.

4. the present Research of ruminally-protected protein process

The fuzzy concept of ruminally-protected albumen (rumen bypass protein) just proposed in nineteen sixties; clearly propose the notion of by-pass protein first up to NRC in 1989, the thick protein in the milk cow forage is divided into cud degrade proteins (RDP) to calendar year 2001 U.S. NRC " dairy cow nutrition requirement " and the non-degrade proteins of cud (RUP) is two distinct components of function in the feed crude protein.Feed thick protein degradable in cud partly is cud degrade proteins (RDP), for microbial growth and microprotein synthetic provides required peptide, free amino acid and ammonia.The non-degraded of cud part can directly enter small intestine in the feed crude protein, and at this by digestion and metabolism.

Nearly 30%～50% protein is not by rumen bacteria and protozoon degraded in the conventional daily ration.This part can be intactly by the dietary protein of cud, is referred to as " bypass protein " (bypass protein) usually or " by-pass protein " (escape-protein).The feed protein cud degradation rate of separate sources is widely different, can be divided three classes usually: cross low (below 40%) of cud value, as soya-bean cake, peanut cake etc.; Cross cud value medium (40%～60%), as cottonseed cake, dehydrated alfalfa powder, corn seed etc.; Cross more than the cud value high (60%), as digested tankage, blood meal, feather meal, bean powder etc.The degradation rate (60%～80%) of protein is higher than the degradation rate (20%～60%) of protein in concentrated feed or the industry byproduct in the common roughage.Feed protein also is subjected to the influence of individuality, feed processing conditions, microbiota etc. at the degradation rate of cud.

Since the nearly more than ten years, Chinese scholars has been launched a large amount of research with regard to the protection of cud albumen.Proposed many guard methods, the method for protection by-pass protein commonly used has at present: formaldehyde protection, tannin protection, NaOH protection, propionic acid protection, method of chemical protection such as ethanol protection; Xeothermic, hot pressing, expanded, the training stir-fry waits heat treatment method; The protein coated for protecting, compound bag quilt, physical protection methods such as polymer coating, but experimental result is all disputable.

Great majority studies show that, utilize the formaldehyde treated protein feeds can significantly reduce protein in the degradation in cud rate, because formaldehyde has toxicity, and it is residual in carcass easily, also can improve concentration of formaldehyde in the milk to cow in milk, the quality of influence breast does not at home and abroad re-use at present substantially.Bridger etc. (1970,1972) have reported that tannin has protected protein matter and avoids microbial degradation, improve the effect of nitrogen retention.But, tannin is the polyhydroxy phenol compounds, very strong polarity is arranged, can irreversible complex reaction take place with protein, in a single day this reaction takes place in protein, can not be decomposed by digestive ferment equally in small intestine, can not discharge amino acid wherein, can not be utilized by animal, not have trophism.Mir (1984) etc. has carried out the naoh treatment soya-bean cake and rape cake is exempted from Research on degradation in cud to protected protein matter.NaOH solution usage when 50% account for dry 2% the time, can significantly reduce the cud degradation rate of protein, the addition of alkali lye was 3% when the cud degradation rate of protein was minimum, when addition increases to 4%, the protection poor effect., find not work during according to (1989) such as Waltz with 5% NaOH protection dregs of beans.If handle dregs of beans with alcohol immersion, though certain effect is arranged, required solution usage is bigger, can't promote the use in production practices.In addition, also had many scholar's research and defended of the rumen-protected effect of chemical substances such as dialdehyde, glyoxal, sodium chloride, propionic acid and this methene one tetramine, but effect is all not satisfactory the good protein feed.

The polyhydroxyketone of selecting for use in the present invention (or polyhydroxy aldehyde) belongs to reduced sugar, and is nontoxic, is used for chemical reagent such as instead of formaldehyde, NaOH, ethanol, tannin and glutaraldehyde, glyoxal, propionic acid, this methene one or four ammoniums to have clear superiority.The effectively degraded of protected protein matter feed in cud on the one hand improves the protein feeds utilization rate, improves the milk composition in the milk, particularly improves the content of lactoprotein; On the other hand, can reduce the discharge capacity of carcass nitrogen, reduce the pollution of large-scale cultivation, especially reduce the animal husbandry breed solid of agricultural is polluted environment.

5. the technical support that gives of this research team

The inventor is engaged in the research of ruminant animal nutrition and forage science for a long time, presides over multinomial major research item, especially does a lot of work aspect proteinaceous nutrient.Team Member of the present invention all possesses sturdy professional knowledge and rigorous scientific research attitude.This invention declare the experiment work that comes from accumulation for a long time and carried out in recent years., the implementation specification rigorous that is provided in the result of use of the present invention, the in vivo studies and the in vitro test of analysis science data from a series of designs.

[summary of the invention]

The present invention adopts the comprehensive treatment method that adds protective agent and suitably heating that protein feed is carried out ruminally-protected and handles.The protective agent of selecting is polyhydroxy aldehyde (or polyhydroxyketone), and mode of heating is the wet method heating.Technical parameter is as follows in the processing technology: heating-up temperature is 60 ℃-140 ℃, and be 30min-120min heat time heating time, and the interpolation concentration of polyhydroxyketone (aldehyde) is 0.5-8% (accounting for the amount of dry matter ratio).Can add a spot of lysine as required in dry run or operational phase.

1. the technical problem that will solve

The present invention is a comprehensive treatment method, consider the positive aspects of heat treated, also will consider the overprotection problem of over-heating to protein.Therefore, the present invention's kind that will propose heating-up temperature, heat time heating time, polyhydroxyketone or polyhydroxy aldehyde with and add the best of breed of concentration.Consider that hydrone activity also has certain influence to the kind and the quantity of the macromolecular compound of Combined Processing generation, determine the moisture that protein raw material is desirable.In addition, because polyhydroxyketone (aldehyde) is in heating process during with proteins react, it is more active that lysine amino participates in the reaction of aldehyde radical of polyhydroxyketone (aldehyde), can cause the lysine of the ruminally-protected albumen of producing to lack.Need to determine that this ruminally-protected albumen substitutes the ratio of the protein raw materials that is untreated aborning, or in following process or the quantity of adding cud lysine in feeding.

2. technical scheme

Condensation reaction takes place in the aldehyde radical of polyhydroxyketone (aldehyde) and the amino of gal4 amino acid at a certain temperature, generates macromolecular compound.This compound can effectively be avoided the degraded of cud to protein, but can be digested by digestive ferment again after entering small intestine.Increase heating-up temperature and reduce the production rate that water activity can increase macromolecule labyrinth thing.Therefore, can effectively improve the cud percent of pass of good protein feed by the adding proportion of control heating-up temperature, heat time heating time, moisture and polyhydroxyketone or aldehyde.With single pepsin solution, two step of pepsin-trypsase enzymatic isolation method, cud Nylon Bag method, the little Nylon Bag method of duodenum and animal experiment the ruminally-protected albumen of producing under this processing technology is carried out effect assessment respectively.

3. effect

Among the present invention, the cud degradation rate 30%-70% can be reduced, the digestible protein quantity that enters small intestine 20%-40% can be improved by selecting different heating-up temperatures, heat time heating time, polyhydroxyketone or polyhydroxy aldehyde addition.Thereby satisfy the proteinaceous nutrient needs of ruminant such as high yield cow, improve its production performance, as the output of milk increase, content of milk protein increase etc. in the milk.In addition, the increase of protein feeds utilization rate can reduce the discharge capacity of carcass nitrogen, reduces environmental pollution, improves the animal husbandry large-scale cultivation solid of agricultural is polluted.Therefore, use of the present invention brings predictable economic benefit and ecological benefits can for animal husbandry and even entire society.

[specific embodiment]

A kind of by handling the method that protein feed improves the milk content of milk protein:

Material: produce both at home and abroad or produce, polyhydroxyketone (aldehyde) comprises D-fructose, D-potassium sorbate, D-wood sugar, D-glucose, D-arabinose, D-galactolipin, D-mannose, D-ribose, D-deoxyribose, L-rhamnose etc.Protein feed comprises that meal expeller and mealsolvents such as dregs of beans, cotton dregs, peanut meal, the certain herbaceous plants with big flowers dregs of rice, rapeseed dregs, the flax dregs of rice and other protein feeds all can be used for composition of the present invention.

The processing and the guard method of protected protein feed are as follows:

1, selects for use polyhydroxyketone (aldehyde) to add in the protein feed, handle through certain technology for protective agent.It is characterized in that: the adding proportion of polyhydroxyketone (aldehyde) is 0.5%-8%, preferred 3%; The heating-up temperature scope is 60 ℃-140 ℃, preferred 90 ℃; Be 30min-150min heat time heating time, preferred 90min.

2, wherein polyhydroxyketone comprises D-fructose, D-potassium sorbate etc.; Polyhydroxy aldehyde comprises D-wood sugar, D-glucose, D-arabinose, D-galactolipin, D-mannose, D-ribose, D-deoxyribose, L-rhamnose etc.Preferred D-wood sugar or D-fructose.

3, protected protein feed comprises meal expeller and mealsolvent and other protein feeds such as dregs of beans, cotton dregs, peanut meal, the certain herbaceous plants with big flowers dregs of rice, rapeseed dregs, the flax dregs of rice.Preferred big dregs of beans or peanut meal.

4, the protein feed according to the preparation of this method the 1st, 2,3 steps is rumen-protected protein feed.

5, Zhi Bei rumen bypass protein feed can be used for the breed of ruminants such as milk cow, milch goat, and the effect that wherein is used for milk cow forage is more remarkable.

When 6, the rumen bypass protein feed being used for dairy cow diet, can partly replace the protein feed that is untreated in the daily ration; Also can all replace other protein feed, it is better to replenish an amount of lysine feeding effect simultaneously.

[experimental example]

Example 1 protected protein feed method screening example

The guard method of rumen bypass protein feed is; in raw material (adopting dregs of beans in this example), spray polyhydroxyketone (or aldehyde) (adopting wood sugar in this example) solution of 0.5%-8%; fully behind the mixing; heating is 20-120 minute under certain 60 ℃ of-140 ℃ of conditions, obtains the rumen-protected protein feed of different disposal.Table 1 is of the present invention group of part processing processed group abridged table of finishing under laboratory condition.

Table 1: the different disposal group abridged table that adopts complex machining process to produce

Annotate: above each processed group is all represented in " numeral/numeral/numeral " mode.For example: combination 105/8/120, the wood sugar that is interpolation 8% under 105 ℃ heats the processed group of 120min.Still expression in this way in the example of back.

The parameter of the middle D-wood sugar addition 3% of example (23), 90 ℃ of temperature, time 90min is handled big dregs of beans in the preferred table 1, obtains rumen-protected dregs of beans.

Example 2 adopts single pepsin enzymatic isolation method to measure the thick protein degradation rate of handling dregs of beans

The protection dregs of beans that obtains in the weighing 0.5g example 1 is put into the 100ml conical flask, adds 30ml and contains the 120IU pepsin, and pH is 1.55 enzyme solutions, at 40 ℃, under the 90rpt/min condition, cultivates 24h with shaking bath.Take out the SAA (sulfosalicylic acid) that the every conical flask in back adds 5ml20%, to the albumen precipitation that is not digested, leave standstill 30min after, filter with middling speed filter paper, the 1%SAA with preheating washes residue then.Residue is together changed in the measuring cup together with filter paper, and baking 24h is to constant weight in 60 ℃ of baking ovens.Measure protein content and calculate its dry disappearance rate and protein degradation rate with Kjeldahl then, see Table 2.

Table 2 dregs of beans is PD rate and and the comparison of control group (single enzyme process) thereof after treatment

	90/1/90	90/3/60	90/3/90	120/1/60	120/3/60	120/3/90	Control group
								CP content (%)	44.21± 0.35	44.18± 0.42	44.16± 0.29	44.13± 0.25	44.18± 0.33	44.27± 0.45	44.31± 0.17
CP degradation rate (%)	63.76± 2.06	64.25± 2.29	62.67± 3.23	64.24± 1.52	64.01± 3.27	53.69± 1.55	87.70± 0.56
								Compare with control group (%) ↓	21.25± 1.31	24.88± 1.42	26.93± 1.79	24.5± 1.04	23.81± 1.91	35.21± 1.05

Example 3 adopts two step of pepsin-trypsase enzymatic isolation method to measure the thick protein degradation rate of handling dregs of beans

The protection dregs of beans that obtains in the weighing 0.5g left and right sides example 1 is put into the 100ml conical flask; add 30ml and contain the 120IU pepsin; pH is 1.55 enzyme solutions; at 40 ℃; under the 90rpt/min condition, after 6h is cultivated in following water-bath, regulate the pH to 7.8 of digestive juice; adding 5ml, to contain the tryptic pH of 160mg be 7.8 enzyme solutions, continues to cultivate 18h at 39 ℃.Take out the SAA (sulfosalicylic acid) that the every conical flask in back adds 5ml 20%, to the albumen precipitation that is not digested, leave standstill 30min after, filter with middling speed filter paper, the 1%SAA with preheating washes residue then.Residue is together changed in the measuring cup together with filter paper, and baking 24h is to constant weight in 60 ℃ of baking ovens.Measure protein content and calculate its dry disappearance rate and protein degradation rate with Kjeldahl then, section processes group data see Table 3.

Table 3 dregs of beans is PD rate and and the comparison of control group (two step enzyme methods) thereof after treatment

	90/1/90	90/3/60	90/3/90	120/1/60	120/3/60	120/3/90	Control group
								CP content (%)	44.21± 0.35	44.18± 0.42	44.16± 0.29	44.13± 0.25	44.18± 0.33	44.27±0.45	44.31± 0.17

CP disappearance rate (%)	72.41± 1.51	73.38± 1.17	71.29± 1.21	68.24± 1.27	69.13± 2.12	70.51± 1.80	91.69± 0.69
								Compare with control group (%) ↓	20.53± 0.95	19.19± 0.88	21.35± 0.95	24.27± 0.98	23.96± 1.40	22.43± 1.24

Example 4 usefulness Nylon Bag methods are measured the thick protein degradation rate in the cud

Select three headbands that the ox of rumen fistula is arranged, body weight is about 500kg.With 3 * 3 latin square experiments, measure the total nitrogen metabolism situation of protection dregs of beans in cud that obtains in the example 1 with the Nylon Bag method.Prepare by keeping 1.3 times of needing in the trophic level of the cow that grows up in NRC (2001) standard, and consider can nitrogen balance.The fine fodder prescription is in the daily ration: corn 49%, dregs of beans 20%, wheat bran 30%, additive premix 1%.Coarse fodder is a corn silage 50% in the daily ration, sheep's hay 50%.Fine fodder: coarse fodder=50: 50.8 and 14 of animal every days feed twice, the fine fodder 3.5kg that feeds every day, coarse fodder 3.5kg can freely drink water.

The preparation method of Nylon Bag and test determination are referring to " ruminant animal nutrition " (Feng Yanglian, 2004).

To be divided into three groups at random through sample, guarantee every group of two Nylon Bags that same sample is all arranged, need every group of bag of record number, to make things convenient for the collection of data.Then, drop in the cud of three oxen record ox number at random.Take out after in cud, placing 24h.With warm water washing 30min (to the water colorless of flushing).Sample take out the back in 60 ℃ to constant weight, record weight, and calculate the dry disappearance rate of respectively handling sample 0h and 24h in cud.Then, measure the Protein content of residue in the Nylon Bag with Kjeldahl, and calculate the degradation rate of each sample at the cud internal protein.After in cud, digesting 12h and 24h respectively, the results are shown in Table 4.

Table 4 dregs of beans is PD rate and and control group thereof in cud after treatment

	90/1/90	90/3/60	90/3/90	120/1/60	120/1/90	120/3/90	Control group
								CP content (%)	44.21± 0.35	44.18± 0.42	44.16± 0.29	44.13± 0.25	44.18± 0.33	44.27± 0.45	44.31± 0.17
12h CP disappears	32.80±	31.94±	26.45±	34.97±	30.08±	27.37±	62.88±

Mistake rate (%) 24hCP disappearance rate (%)	1.23 43.78± 2.11	2.08 45.72± 2.31	2.94 41.39± 2.17	1.67 43.23± 1.95	2.13 41.72± 2.31	1.79 45.34± 2.03	2.65 86.34± 2.79
								24h dCP VS control group (%) ↓	45.01± 2.45	43.17± 2.55	47.43± 2.48	45.48± 2.37	47.17± 2.55	38.59± 2.41

Example 5 usefulness motion Nylon Bag methods are measured the thick protein absorptivity in the small intestine

Select three headbands that the ox of duodenum fistula is arranged, body weight is about 500kg.With 3 * 3 latin square experiments, measure the protection dregs of beans that obtains in the example 1 in enteral protein adsorption situation with little Nylon Bag method.Test ox daily ration is referring to example 3 preparations.Working sample takes by weighing about 1 gram and puts into little Nylon Bag for handling the residue after the dregs of beans cud is cultivated 12 hours.Little Nylon Bag aperture is the nylon material of 42 microns (suggestion is used 11 microns), and (trial test adopts 3 * 3cm2) to bag area 6 * 6cm2, the sack elevated-temperature seal.Dropping into the sportsbag that needs to fill testing sample before the duodenum and be immersed in the hydrochloric acid solution that pH value is 2.4 0.004mol/ml 1 hour, is cultivation 2 hours in hydrochloric acid-pepsin solution (hydrochloric acid solution of 100mg pepsin 1: 1000L-1) of 2.4 in the pH value then.Nylon Bag after will handling then (in advance in warm water soak youngster minute) is put into corresponding experiment calf intestinal respectively by the duodenum fistula, puts into 1 every 15min.From ight soil, reclaim Nylon Bag on time.Mobile Nylon Bag is placed under the standard running water thread flushing, clear until Nylon Bag water, tasteless till.At last, residue is put into the title sample ware of constant weight, place 105 ± 2 ℃ the interior baking of baking oven to take out in 3.5 hours, in drier, cool off 30min, weigh.Measure the Protein content of residue in the motion Nylon Bag then with Kjeldahl, and calculate the absorptivity of each sample, see Table 5 at the small intestine internal protein.

Table 5 dregs of beans is PD rate and and control group thereof in small intestine after treatment

	90/1/90	90/3/60	90/3/90	120/3/60	120/1/90	120/3/90	Control group
								CP content (%)	44.21± 0.35	44.18± 0.42	44.16± 0.29	44.13± 0.25	44.18± 0.33	44.27± 0.45	44.31± 0.17
CP digestibility (%)	71.60± 2.34	69.26± 3.01	64.85± 3.24	63.18± 2.65	76.35± 1.67	73.51± 2.35	90.23± 1.97
								CP absorptivity (%)	48.42± 1.35	48.28± 2.01	49.67± 1.64	50.34± 2.39	54.68± 2.17	49.10± 1.97	24.10± 1.69
CP absorptivity VS control group (%) ↑	22.63± 1.52	22.49± 1.85	25.57± 1.66	26.62± 2.04	30.58± 1.93	25.00± 1.88

In addition; the present invention also adopts this processing technology that protein feeds such as cotton dregs, peanut meal, the certain herbaceous plants with big flowers dregs of rice are protected processing, and result of the test all shows, after the Combined Processing of this technology; can effectively avoid the degraded of protein feeds in cud, improve the digestible protein quantity that enters small intestine.

Example 6 usefulness animal experiment methods are measured and are handled the influence of dregs of beans to the milk milk protein content

Do animal experiment with milk cow, the protection dregs of beans that obtains in the example 1 is used in the lactating cow feed.Select 40 cows in milk for use, be divided into 4 processing, handle to be respectively 1,2,3 group of control group and test, handle dregs of beans and substitute 0%, 15%, 30%, 45% of dregs of beans in the cow concentrated feed respectively.The result shows that the cow in milk feed contains the concentrated feed of handling dregs of beans, and the protein content in the fresh milk is compared with control group can improve 5-30%.

Claims (6)

1. one kind by handling the method that protein feed improves milk protein content.Add in the protein feed as protective agent with polyhydroxyketone or polyhydroxy aldehyde, through handling the protein feed that is protected.It is characterized in that: the addition of polyhydroxyketone or polyhydroxy aldehyde is at 0.5%-8%; Heating-up temperature is 60 ℃-140 ℃; Be 30min-120min heat time heating time.

2. described a kind of by handling the method that protein feed improves milk protein content according to claim 1, wherein polyhydroxyketone comprises D-fructose, D-potassium sorbate etc.; Polyhydroxy aldehyde comprises D-wood sugar, D-glucose, D-arabinose, D-galactolipin, D-mannose, D-ribose, D-deoxyribose, L-rhamnose etc.

3. described a kind of by handling the method that protein feed improves milk protein content according to claim 1, wherein processed protein feed comprises protide feeds such as dregs of beans, cotton dregs, peanut meal, the certain herbaceous plants with big flowers dregs of rice, rapeseed dregs, the flax dregs of rice.

4. the protein feed of claim 1 described method preparation is protected protein feed or rumen-protected protein feed.

5. according to the protected protein feed that requires 4 preparations with all strength, can in milk cow, milch goat and other animal-breedings, use.

6. when according to claim 5 the rumen bypass protein feed being used for dairy cow diet, can partly replace the protein feed that is untreated in the daily ration; Also can all replace other protein feed, it is better to replenish an amount of lysine feeding effect simultaneously.