CN1936567A - Technology for appraising acipenser schrencki and Acipenser datryanus - Google Patents
Technology for appraising acipenser schrencki and Acipenser datryanus Download PDFInfo
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- CN1936567A CN1936567A CN 200510010346 CN200510010346A CN1936567A CN 1936567 A CN1936567 A CN 1936567A CN 200510010346 CN200510010346 CN 200510010346 CN 200510010346 A CN200510010346 A CN 200510010346A CN 1936567 A CN1936567 A CN 1936567A
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- acipenser
- sturgeon
- ahn
- schrencki
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Abstract
This is one kind appraisal technology of Acipenser schrenki Brandt and Huso dauricus. It exams the plants specificity DNA mark AHN in Acipenser schrenki Brandt, Huso dauricus and its products DNA, the AHN primer size is 10bp, the alkali base sequence is 'CACCCGGATG'. Use this primer on Acipenser schrenki Brandt, Huso dauricus and their products DNA and PCR examination. Acipenser schrenki Brandt receives 200bp zone electrophoresis, but Huso dauricus. The two kinds of fish and their products can be identificated appropriately.
Description
Technical field
The invention belongs to the kind authenticate technology field of species, is to utilize molecular labeling to carry out the kind authenticate technology of sturgeon section fish by it.
Background technology
Sturgeon is the fish of individual maximum, longest-lived in the freshwater fish, and China just has 8 kinds such as acipenser schrencki, Da Shi huso sturgeon within the border.Its meat flavour deliciousness, nutritious, whole body is precious.Because its stock number descends, this population is endangered in recent years.Protective development is all being carried out to it in countries in the world.Ration export is identified to the species that the sturgeon product of selling carries out dna level in the international market.Purpose is exactly to understand the existing amount of fishing of sturgeon and then understand its stock number, formulates safeguard measure and slows down its extinction speed.China has only acipenser schrencki and Da Shi huso sturgeon that certain output is arranged at present.
The sturgeon series products is carried out the DNA species identify that mainly utilizing cytochrome b gene can show these characteristics of polymorphism on variety classes carries out DNA detection to the sturgeon series products abroad, but this detection method is at present also unexposed.The testing agency of China also can't utilize this technology to carry out this work, does not also have the technology of independent development.Therefore develop a kind of China testing agency laboratory that is suitable for to the technology that the sturgeon product detects, break the blockade on new techniques of we being carried out abroad, improve China's sturgeon series products competitive power in the international market and be very important.And the dna molecular marker that obtains to have species specificity in acipenser schrencki, Da Shi huso sturgeon is the key of carrying out this work.
Summary of the invention
In order to obtain in acipenser schrencki, Da Shi huso sturgeon, to have the dna molecular marker of species specificity, utilization of the present invention has polymorphism between kind random primer molecular labeling carries out the screening of species specificity molecular labeling to acipenser schrencki, Da Shi huso sturgeon, has obtained to have the species specificity molecular labeling between acipenser schrencki, Da Shi huso sturgeon.
The technical scheme that the present invention deals with problems is: the random primer that 1, utilizes synthetic 10 bases of dna synthesizer, utilize round pcr that the genomic DNA of acipenser schrencki, Da Shi huso sturgeon and products thereof has been carried out pcr amplification, obtained a random primer molecular labeling AHN who in these two kinds of fishes, has species specificity, AHN primer size is 10bp, and base sequence is cacccggatg.With this primer acipenser schrencki, Da Shi huso sturgeon are carried out PCR and detect, acipenser schrencki can obtain the 200bp electrophoresis band, and the Da Shi huso sturgeon does not then have this band.Reach the purpose that two kinds of fishes and products thereof are reflected, identify.
The invention has the beneficial effects as follows that obtained one and have the species specificity molecular labeling between acipenser schrencki, Da Shi huso sturgeon, this mark can be used for importing and exporting the qualitative detection of sturgeon product, also can be used for the Germplasm Identification in the scientific research simultaneously.
Claims (2)
1. dna molecular marker AHN who in acipenser schrencki, Da Shi huso sturgeon and products thereof DNA detection, has species specificity, it is characterized in that: AHN primer base sequence is cacccggatg, with this mark acipenser schrencki, Da Shi huso sturgeon being carried out PCR detects, acipenser schrencki can obtain the 200bp electrophoresis band, and the Da Shi huso sturgeon does not then have this band.
2. according to the claim 1 described dna molecular marker AHN that has species specificity in acipenser schrencki, Da Shi huso sturgeon detect, it is characterized in that: AHN primer size is 10bp, and base sequence is cacccggatg.With this primer acipenser schrencki, Da Shi huso sturgeon and products thereof DNA is carried out PCR and detect, acipenser schrencki can obtain the 200bp electrophoresis band, and the Da Shi huso sturgeon does not then have this band.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510010346 CN1936567A (en) | 2005-09-19 | 2005-09-19 | Technology for appraising acipenser schrencki and Acipenser datryanus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510010346 CN1936567A (en) | 2005-09-19 | 2005-09-19 | Technology for appraising acipenser schrencki and Acipenser datryanus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1936567A true CN1936567A (en) | 2007-03-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510010346 Pending CN1936567A (en) | 2005-09-19 | 2005-09-19 | Technology for appraising acipenser schrencki and Acipenser datryanus |
Country Status (1)
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| CN (1) | CN1936567A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103276092A (en) * | 2013-06-07 | 2013-09-04 | 中国水产科学研究院长江水产研究所 | Molecular identification method of four major Chinese carps in fresh water |
| CN103436611A (en) * | 2013-08-20 | 2013-12-11 | 徐鹏 | PCR-RFLP rapid detection method for common sturgeons |
| CN103436610A (en) * | 2013-08-20 | 2013-12-11 | 徐鹏 | PCR-RFLP rapid detection method for common sturgeons |
| CN105002170A (en) * | 2015-07-31 | 2015-10-28 | 中国长江三峡集团公司 | Amur sturgeon germplasm molecular marker identification kit and application thereof |
| CN105063192A (en) * | 2015-07-31 | 2015-11-18 | 中国长江三峡集团公司 | Molecular marker for identifying amur sturgeon germplasm and application of molecular marker |
| CN108531620A (en) * | 2018-06-29 | 2018-09-14 | 四川农业大学 | Identify the primer and method of Amur Sturgeon, siberia platform and its filial generation |
| CN108660224A (en) * | 2018-06-29 | 2018-10-16 | 四川农业大学 | Identify the primer and method of siberia platform, Amur Sturgeon and its filial generation |
| CN108660223A (en) * | 2018-06-29 | 2018-10-16 | 四川农业大学 | Siberia platform and to the west of miscellaneous identification method |
| CN108866203A (en) * | 2018-07-06 | 2018-11-23 | 江汉大学 | A kind of hybrid sturgeon paternity test microsatellite marker primer and its hybrid sturgeon paternity test method |
| CN109182531A (en) * | 2018-07-06 | 2019-01-11 | 江汉大学 | A kind of hybrid sturgeon Individual identification kit and its application |
-
2005
- 2005-09-19 CN CN 200510010346 patent/CN1936567A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103276092A (en) * | 2013-06-07 | 2013-09-04 | 中国水产科学研究院长江水产研究所 | Molecular identification method of four major Chinese carps in fresh water |
| CN103436611A (en) * | 2013-08-20 | 2013-12-11 | 徐鹏 | PCR-RFLP rapid detection method for common sturgeons |
| CN103436610A (en) * | 2013-08-20 | 2013-12-11 | 徐鹏 | PCR-RFLP rapid detection method for common sturgeons |
| CN105063192B (en) * | 2015-07-31 | 2017-08-25 | 中国长江三峡集团公司 | Molecular labeling and its application for identifying Amur Sturgeon germplasm |
| CN105063192A (en) * | 2015-07-31 | 2015-11-18 | 中国长江三峡集团公司 | Molecular marker for identifying amur sturgeon germplasm and application of molecular marker |
| CN105002170B (en) * | 2015-07-31 | 2017-08-01 | 中国长江三峡集团公司 | Amur Sturgeon germplasm molecular markers for identification kit and its application |
| CN105002170A (en) * | 2015-07-31 | 2015-10-28 | 中国长江三峡集团公司 | Amur sturgeon germplasm molecular marker identification kit and application thereof |
| CN108531620A (en) * | 2018-06-29 | 2018-09-14 | 四川农业大学 | Identify the primer and method of Amur Sturgeon, siberia platform and its filial generation |
| CN108660224A (en) * | 2018-06-29 | 2018-10-16 | 四川农业大学 | Identify the primer and method of siberia platform, Amur Sturgeon and its filial generation |
| CN108660223A (en) * | 2018-06-29 | 2018-10-16 | 四川农业大学 | Siberia platform and to the west of miscellaneous identification method |
| CN108660224B (en) * | 2018-06-29 | 2021-10-29 | 四川农业大学 | Primer and method for identifying Siberian sturgeons, Acipenser schrenki and filial generations thereof |
| CN108866203A (en) * | 2018-07-06 | 2018-11-23 | 江汉大学 | A kind of hybrid sturgeon paternity test microsatellite marker primer and its hybrid sturgeon paternity test method |
| CN109182531A (en) * | 2018-07-06 | 2019-01-11 | 江汉大学 | A kind of hybrid sturgeon Individual identification kit and its application |
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