CN1936545A - On-line monitoring-detecting chemical light-illuminating detector for contaminent in aquatic article sample - Google Patents
On-line monitoring-detecting chemical light-illuminating detector for contaminent in aquatic article sample Download PDFInfo
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- CN1936545A CN1936545A CN 200510086514 CN200510086514A CN1936545A CN 1936545 A CN1936545 A CN 1936545A CN 200510086514 CN200510086514 CN 200510086514 CN 200510086514 A CN200510086514 A CN 200510086514A CN 1936545 A CN1936545 A CN 1936545A
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Abstract
This invention relates to a chemical luminous detector used in on-line monitoring/detecting pollutants in water samples including: a fluid transportation part, a chemical luminous monitor-detect part and a control part of a working control machine, in which, the reagent and a water sample to be tested enter into the screw channel of the chip of the flow cell of the detection part via their transporting parts then to be discharged from the outlet of waste solution after being mixed, the screw channel of the flow cell chip is opposite to the photosensitive window of a multiplier phototube and the original signals are connected with the input of the work control machine via a high resistance operation amplifier, a high frequency modem, a signal processor and a 16 A/D converter.
Description
Technical field
The present invention relates to the chemiluminescent analyzer that uses chemiluminescence analysis to carry out a kind of full-automatic, the online prison/detection of water pollutant.It particularly is exactly a kind of chemiluminescence detector that is used for the online prison of water body example pollutant/detection prison/detection, that use chemiluminescence that can be used for water pollutants such as seawater, river, tap water, potable water, sanitary sewage, industrial waste water.Its pollutant can comprise inorganic pollutant, also can include organic pollutants.
Background technology
Chemiluminescence and bioluminescence are the ubiquitous phenomenons of occurring in nature, though some chemiluminescence and bioluminescence found by people very early, deep research is carried out in its reaction mechanism and analytical applications aspect then starts from the sixties.On the one hand because the development of modern electronic technology and highly sensitive photoelectric sensor, for research and measure chemiluminescence, bioluminescence provides new effective means; On the other hand, because the rise of life science, environmental science and material science, to the demand of analytical test means, also the development to this research field has played important impetus.
Instrument simply is a major advantage of chemiluminescence analysis.The general requirement of chemiluminescence measuring instrument has following several sections, and promptly photodetector, sampling system, signal output system and data handling system (comprising that signal amplifies) also have beam splitting system in some cases.At home and abroad the commercial apparatus of more existing different models is sold at present.
Domestic chemiluminescence detector has:
The IFFM-D type portable injection chemiluminescence analyser of Xi'an Rui Mai Analytical Instrument Co., Ltd and MPI-B type multiparameter chemiluminescent assay system.MPI-B type multiparameter chemiluminescent assay system is that Xi'an Rui Mai electronics scientific technology co develops, based on the high-performance analysis test apparatus of WINDOWS system operation platform.IFFM-D type portable injection chemiluminescence analyser is the domestic full-automatic chemical luminous detection analytic system of releasing the earliest.Faint chemistry of the BPCL of Instite of Biophysics, Chinese Academy of Sciences's development and production and bioluminescence measuring instrument, the Chemiluminescence Apparatus of the type mainly are applicable to the research of static chemiluminescence and chemiluminescence dynamics and chemiluminescence mechanism.
External chemiluminescence detector has:
The LD400 chemiluminescence detector of U.S. Beckman Coulter Inc., the LUMIstar Chemiluminescence Apparatus of Germany BMG company, FB12 single hose autoluminescence analyser, FB14 portable luminescence analyzer, Sirius single hose autoluminescence analyser, SmartLine TL single hose autoluminescence analyser, the board-like autoluminescence analyser of MPL1, the board-like autoluminescence analyser of Orion II high sensitivity of Germany BERTHOLD company, the BHP9502 of Binsong Photon Technology Co., Ltd. Beijing, BHP9503 type faint light analyser; The NicholsAdvantage full-automatic chemical light-emitting appearance that U.S. Nichols Institute Diagnostics produces; These above-mentioned light-emitting appearances are board-like or the tubular type chemiluminescence detector, can not realize real full-automatic, on-line monitoring/detection, still belong to off-line monitoring/detection.
Summary of the invention
The object of the present invention is to provide a kind of chemiluminescence detector that is used for the online prison of water body example pollutant/detection; Industrial computer control section, chemiluminescence detection part and reagent transport portion are combined into one; Each part all is fixed on the control framework firmly, very easily adapt to operation under abominable external environment condition, complete machine antidetonation, diamagnetic, shock resistance, anti-ly tilt to wave, anti-vibration, protection against the tide, decay resistance be good, applied widely, prison/detection applicable to the inorganic pollutant in the various water bodys such as seawater, river, tap water, potable water, sanitary sewage, industrial waste water, also the analysis applicable to the prison/detection, particularly various trace materialss of organic contaminant has highly sensitive and characteristics that detectability is low, the range of linearity is wide; The making compactness of complete machine, space utilization and distribution are rationally, and be convenient; Easy to operate, it is convenient rapidly to analyze, and the efficient height has unprecedented advantage and wide application prospect in the water quality monitoring/context of detection of environmental pollution monitoring/detection and extensive area.
Technical scheme of the present invention is as follows:
The chemiluminescence detector that is used for the online prison of water body example pollutant/detection provided by the invention comprises: FLUID TRANSPORTATION part I, chemiluminescence prison/test section II and industrial computer control section III;
Described FLUID TRANSPORTATION part I comprises: reagent transport portion I
1With tested water body sample delivery part I
2
Described reagent transport portion I
1Comprise: the first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline and n reagent transfer pipeline that two ends link to each other with discharge pump 6 with the container that different reagent are housed respectively, n=2-10; The first reagent transfer solenoid valve 1, the second reagent transfer solenoid valve 2, the 3rd reagent transfer solenoid valve 3, the 4th reagent transfer solenoid valve 4 and n reagent transfer solenoid valve that the described reagent of control is carried are housed respectively on the described first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline and the n reagent transfer pipeline;
Described tested water body sample delivery part I
2Comprise: the tested water body sample delivery pipeline that two ends link to each other with peristaltic pump 6 with the container that the tested water body sample is housed respectively, be equipped with the tested water body sample delivery solenoid valve of this tested water body sample delivery of control on the described tested water body sample delivery pipeline;
Described chemiluminescence monitoring/test section II comprises: circulation a chamber chip 7 and a photomultiplier that links to each other with power supply 8, described power supply 81 operating voltage by city's alternating current transformer be converted to-700～-1100V; The photosensitive window of described photomultiplier 8 is relative with the helical duct of flow cell chip 7; Flow cell chip 7 is by first substrate that is carved with helical duct, second substrate that is carved with two parallel beeline channels and three layers of bonding chip that channel-less cover plate is formed; The material of flow cell chip 7 can be silicon chip, glass, quartz and organic polymer, a kind of as in epoxy resin, polymethylmethacrylate, polycarbonate, the dimethyl silicone polymer;
After described first reagent transfer pipeline that is connected with peristaltic pump 6 output terminals and the 3rd reagent transfer pipeline converge, be connected with the first inlet a of flow cell chip 7 again; After the described second reagent transfer pipeline that is connected with peristaltic pump 6 output terminals, the 4th reagent transfer pipeline mix by the glass three-way pipe, converge the back with the 5th tested water body sample delivery pipeline again and be connected with the second inlet b of flow cell chip 7; Enter described first the inlet a reagent and enter second the inlet b mixed liquor in the helical flow path of flow cell chip 7, mix after, discharge by waste liquid outlet c;
Described industrial computer control section III comprises: control the described first reagent transfer solenoid valve 1, the second reagent transfer solenoid valve 2, the 3rd reagent transfer solenoid valve 3, the first control circuit 10 that the 4th reagent transfer solenoid valve 4 and n reagent transfer solenoid valve are opened or closed, control the second control circuit 20 that the 5th tested water body sample delivery solenoid valve opens or cuts out, high resistance operating amplifier 30, high frequency modulated detuner 40, signal processor 50, sixteen bit analog to digital converter 60 and industrial computer 70;
Described photomultiplier 8 output terminals link to each other with the input end of high resistance operating amplifier 30; First control circuit 10 input ends link to each other with industrial computer 70 output terminals respectively with second control circuit 20 input ends; Described signal processor 50 links to each other with industrial computer 70 input ends by sixteen bit analog to digital converter 60.
The chemiluminescence detector that is used for the online prison of water body example pollutant/detection of the present invention also comprises a monoblock type outside framework, the inner space that is made of three layers of cross direction profiles two-layer bulkhead bracket that is provided with of described monoblock type outside framework; Described industrial computer control section III places the superiors; Chemiluminescence prison/test section II places the middle layer of complete machine; Described FLUID TRANSPORTATION part I places bottom.
Chemiluminescence and high pressure are set on the front panel of described monoblock type outside framework change monitor window, and power switch, dual pressure control switch, high voltage adjusting knob, sensitivity adjusting knob, inhibition adjusting knob, baseline coarse adjustment knob and baseline vernier adjustment knob have been installed simultaneously, realized manually and automatic double selection.Described whole outside framework can use various steel plates such as stainless steel, cold-rolled steel, accomplishes both firm, corrosion-resistant again, inexpensive; The limit pin uses three angular rolling iron, helps and other external frame case amalgamation, is beneficial to operation under the extraneous rugged surroundings; The work control of complete machine, time control, information transmission and reception control assign to finish by the industrial computer control part, the industrial computer control section had both had the ease for operation of common computer, had extremely strong stability and antidetonation, diamagnetic, shock resistance again, anti-ly tilted to wave, anti-vibration, protection against the tide, decay resistance be good; The basic design principle of chemiluminescence detector part can be summarized as follows: (1) high-gain, and low noise, the photomultiplier that the stable high voltage power supply is arranged is to guarantee sensitive and to reappear ground measuring light radiation; (2) high-gain highly sensitive, that the range of linearity is wide, the direct current amplifier of high impedance; (3) have the means of the electric signal after quantitative measurement is amplified; (4) have the extraneous light of preventing and bleed, and to have the reaction chamber that is suitable for reagent mix, this mixing and reaction chamber to adopt the applicant's the patent No. be the flow cell chip of ZL02 2 38063.9.The chip of three layers of bonding that its flow cell chip 7 is made up of first substrate that is carved with helical duct, second substrate that is carved with two parallel beeline channels and channel-less cover plate, flow cell chip material can be silicon chip, glass, quartz and organic polymer, a kind of as among epoxy resin, polymetylmethacrylate, polycarbonate, the dimethyl silicone polymer PDMS; The structure of flow cell chip as shown in Figure 2.The reagent transport portion then is that the solenoid valve of pipeline control section and reagent and reagent delivery device are incorporated into in one deck, saved the use length of analyzing needed time and connecting pipe, reached quick, accurate, online requirement, that transfer pipeline adopts is acidproof, alkaline-resisting, corrosion-resistant, anti-aging, the silicone tube of long service life, and connecting pipe has then adopted the pvc pipe of same acidproof, alkaline-resisting, corrosion-resistant, anti-aging, long service life.Simultaneously, on arrangement space, the industrial computer that will be used for the complete machine Control work partly places the superiors, makes it use height and experimental work person's height of sighting line to be complementary, and is convenient to operate and monitor the duty of complete machine; Chemiluminescence detector places the center section of complete machine, has saved the use length that reagent is carried connecting pipe, has further also saved the needed time of analyzing; The reagent transport portion places the bottom of complete machine, is convenient to the replacing of solution and transfer pipeline; Simultaneously, taken into full account the safe handling problem of complete machine, avoided because the leak of liquid that careless manipulation causes to the hidden danger that complete machine may bring, has carried out detailed human oriented design to the use of complete machine, maintenance and operation personnel's safety.
The present invention compared with prior art has following advantage:
The present invention is with chemiluminescence detector and systems control division divides and reagent delivery device combines, and has formed online, the full automatic advanced scientific instrument of reagent conveying, detection, data processing and remote control four one.Compare with known chemiluminescence detector, the complexity of the preceding various softwares of the work that need not, pipeline, data processing circuit, communication line connects, manual intervention is few, simple, convenient, the detection efficiency height, can realize online, detect a plurality of projects simultaneously, speed is fast, the scope of application is wider, can be according to different needs and water quality requirement, finish various types of, from simple to the analytical work of complexity, design hommization more.Each part all is fixed on the control framework firmly, very easily adapt to operation under abominable external environment condition, complete machine antidetonation, diamagnetic, shock resistance, anti-ly tilt to wave, anti-vibration, protection against the tide, decay resistance be good, can be equipped on various types of inspection vehicles; Applied widely, applicable to the monitoring/detection of the pollutant in the various water bodys such as seawater, river, tap water, potable water, sanitary sewage, industrial waste water; The making compactness of complete machine, space utilization and distribution are rationally, and be convenient; Easy to operate, it is convenient rapidly to analyze, and the efficient height has unprecedented advantage and wide application prospect in the monitoring water quality on line/context of detection of environmental pollution monitoring/detection and extensive area.
Description of drawings
Fig. 1 is a fundamental diagram of the present invention;
Fig. 2 is the structural representation of flow cell chip 7;
Fig. 3 is analysis process figure of the present invention.
Embodiment:
1, industrial computer control section: industrial computer need not any repacking, use the software (this software translating is very easy to accomplish for the those of ordinary skill of software technology field) of compiling voluntarily that instruction and monitoring are sent in the work of complete machine, utilize existing three data serial ports, one of them serial ports links to each other with the Long-distance Control line, is used for remote control and sends result data; Two serial ports in addition then link to each other with the solenoid control element that places Chemiluminescence Apparatus inside, are used for the conveying of reagent and the control of mixture length.
2, the setting of Chemiluminescence Apparatus: optical sensor uses low noise, high sensitivity, wide wavelength coverage photomultiplier, photoelectricity transformation principle according to photomultiplier, power transformer adopts current noise little, output stable power transformer, and adopt special dustproof, electrostatic prevention, antijamming measure; Signal acquiring board is brief stable, detects good stability for a long time, and anti-external environment capability of influence is strong, perfect heat-dissipating.Original signal enters industrial computer 70 through high resistant computing amplification appliance 30, high frequency modulated detuner 40, signal processor (filtering, slicing etc.) 50, sixteen bit analog to digital converter 60 after handling.
Chemiluminescence and high pressure are set on the front panel of monoblock type outside framework change monitor window, and power switch, dual pressure control switch, high voltage adjusting knob, sensitivity adjusting knob, inhibition adjusting knob, baseline coarse adjustment knob and baseline vernier adjustment knob have been installed simultaneously, realized manually and automatic double selection.The Chemiluminescence Apparatus size is fixed in the complete machine cabinet by screw with long 480mm * wide 150mm * thick 1.5mm cold-rolled steel sheet with the front portion for long 400mm * wide 440mm * high 20mm;
3, reagent transport portion: the long 600mm of reagent transport portion * wide 440mm * high 150mm, the front portion is fixed with the cold-rolled steel sheet of long 480mm * wide 300mm * thick 1.5mm.The front is locked in the complete machine cabinet with rotation lock, and both sides are connected and fixed on complete machine cabinet both sides with slide rail, are convenient to push-and-pull.The reagent transport portion comprises even separated reagent frame, i.e. reagent container is as first reagent container 11, second reagent container 22, the 3rd reagent container 33 and the 4th reagent container 44 among Fig. 3; Its size is the grid of long 100mm * wide 100mm * high 150mm, and separator material is a cold-rolled steel; Also can become littler or bigger difform spacing frame according to different needs and actual conditions.Four of installations or more solenoid valve are set on the next door of spacing frame, as the first reagent transfer solenoid valve 1, the second reagent transfer solenoid valve 2, the 3rd reagent transfer solenoid valve 3 and the 4th reagent transfer solenoid valve 4 among Fig. 3, be used to control the conveying of reagent, the selection of its position help shortening delivery of therapeutic agents pipeline length and save analysis time.Reagent delivery device adopts inexpensive, easy and simple to handle peristaltic pump, and the characteristics of this pump are stable, accurate, and noise is little, and pulse is little, and shockproof rotating speed height is low in energy consumption, but not only manual operation of rotational speed regulation, and outer control interface is provided, control automatically; Its pump line use is acidproof, alkaline-resisting, corrosion-resistant, anti-aging, the silicone tube of long service life.The assembling of three parts combines, and has saved the instrument usage space, has detected the time that arrives Chemiluminescence Apparatus, has avoided too tediously long pipeline to connect.
According to the assembling of promptly having finished the water pollutant chemiluminescence detector with upper type.Its structure as shown in figures 1 and 3, the chemiluminescence detector that is used for the online prison of water body example pollutant/detection provided by the invention comprises: FLUID TRANSPORTATION part I, chemiluminescence prison/test section II and industrial computer control section III;
Described FLUID TRANSPORTATION part I comprises: reagent transport portion I
1With tested water body sample delivery part I
2
Described reagent transport portion I
1Comprise: the first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline and n reagent transfer pipeline that two ends link to each other with discharge pump 6 with the container that different reagent are housed respectively, n=2-10; The first reagent transfer solenoid valve 1, the second reagent transfer solenoid valve 2, the 3rd reagent transfer solenoid valve 3, the 4th reagent transfer solenoid valve 4 and n reagent transfer solenoid valve that the described reagent of control is carried are housed respectively on the described first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline and the n reagent transfer pipeline;
Described tested water body sample delivery part I
2Comprise: the tested water body sample delivery pipeline that two ends link to each other with peristaltic pump 6 with the container that the tested water body sample is housed respectively, be equipped with the 5th tested water body sample delivery solenoid valve of this tested water body sample delivery of control on the described tested water body sample delivery pipeline; As first sampling receptacle 51, second sampling receptacle 52 and the 3rd sampling receptacle 53 among Fig. 3; Its with pipeline that peristaltic pump 6 is connected on the first sample delivery solenoid valve 511, the second sample delivery solenoid valve 522 and the 3rd sample delivery solenoid valve 533 are housed respectively;
Described chemiluminescence monitoring/test section II comprises: circulation a chamber chip 7 and a photomultiplier that links to each other with power supply 8, described operating supply voltage by city's alternating current transformer be converted to-700～-1100V; The photosensitive window of described photomultiplier 8 is relative with the helical duct of flow cell chip 7; Flow cell chip 7 is by first substrate that is carved with helical duct, second substrate that is carved with two parallel beeline channels and three layers of bonding chip that channel-less cover plate is formed; The material of flow cell chip 7 can be silicon chip, glass, quartz and organic polymer, a kind of as in epoxy resin, polymethylmethacrylate, polycarbonate, the dimethyl silicone polymer;
After described first reagent transfer pipeline that is connected with peristaltic pump 6 output terminals and the 3rd reagent transfer pipeline converge, be connected with the first inlet a of flow cell chip 7 again; After the described second reagent transfer pipeline that is connected with peristaltic pump 6 output terminals, the 4th reagent transfer pipeline mix by the glass three-way pipe, converge the back with the 5th tested water body sample delivery pipeline again and be connected with the second inlet b of flow cell chip 7; After the mixed liquor that enters the reagent of the described first inlet a and enter the second inlet b mixes in the helical flow path of flow cell chip 7, with waste liquid outlet c discharge;
Described industrial computer control section III comprises; Second control circuit 20, high resistance operating amplifier 30, high frequency modulated detuner 40, signal processor 50, sixteen bit analog to digital converter 60 and the industrial computer 70 controlling the described first reagent transfer solenoid valve 1, the second reagent transfer solenoid valve 2, the 3rd reagent transfer solenoid valve 3, the 4th reagent transfer solenoid valve 4 and the unlatching of n reagent transfer solenoid valve or the first control circuit 10 of closing, the unlatching of control the 5th tested water body sample delivery solenoid valve or close;
Described photomultiplier 8 output terminals, first control circuit 10 output terminals and second control circuit 20 output terminals link to each other with industrial computer 70 output terminals respectively; Described signal processor 50 links to each other with industrial computer 70 input ends by sixteen bit analog to digital converter 60.
The chemiluminescence detector that is used for the online prison of water body example pollutant/detection of the present invention also comprises a monoblock type outside framework, the inner space that is made of three layers of cross direction profiles two-layer bulkhead bracket that is provided with of described monoblock type outside framework; Described industrial computer control section III places the superiors; Chemiluminescence prison/test section II places the middle layer of complete machine; Described FLUID TRANSPORTATION part I places bottom.
Fig. 2 is the structural representation of the flow cell chip of ZL02 2 38063.9 for the applicant's the patent No.;
The chemiluminescence detector that is used for the online prison of water body example pollutant/detection of the present invention also comprises a monoblock type outside framework, the inner space that is made of three layers of cross direction profiles two-layer bulkhead bracket that is provided with of described monoblock type outside framework; Described industrial computer control section III places the superiors; Chemiluminescence prison/test section II places the middle layer of complete machine; Described FLUID TRANSPORTATION part I places bottom.
Be that example is described with uranin and Determination of Phenol below, use chemiluminescence detector of the present invention to detect the application of water sample pollutant:
Respectively with sample or standard and sodium hypochlorite and superoxol by corresponding pipeline input analytic system, water pollutant chemiluminescence detector fundamental diagram and analysis process figure see Fig. 1 and Fig. 3: empty frame I partly is the reagent transport portion among the figure, empty frame II partly is the Chemiluminescence Apparatus part, and empty frame III partly is the systems control division branch.
In first reagent container 11 liquor natrii hypochloritis is housed; In the 3rd reagent container 33 superoxol is housed; In second reagent container 22 liquor potassic permanganate is housed; In the 4th reagent container 44 alkaline luminol solution is housed; The water body example or the standard solution of collection in worksite are housed in the 5th sampling receptacle 51,51,53.If water body example comparatively muddiness or floating thing is too much, can with the device that the is used for sampling by filtration collection site water body example that directly links to each other.
First reagent transfer solenoid valve 1 of the first reagent transfer pipeline and the 3rd reagent transfer solenoid valve 3 of the 3rd reagent transfer pipeline are opened simultaneously, are used for measuring the uranin of uranin standard solution or water body example; Second reagent transfer solenoid valve 2 of the second reagent transfer pipeline and the 4th reagent transfer solenoid valve 4 of the 4th reagent transfer pipeline are opened simultaneously, are used for the phenols of on-the-spot detecting/monitoring phenol standard solution or water body example;
According to Fig. 1 and each road pipeline that connects shown in Figure 3, press the peristaltic pump pipeline solid, open water pollutant luminous detection instrument, after luminous software moves voluntarily and opens, click " start and gather ", open peristaltic pump, note the direction that solution is carried, the throughput direction of solution should be for being flowed to the direction of chemiluminescence detecting/monitoring instrument part II by first reagent container 11, the 3rd reagent container 33, observe the solution traffic condition of each pipeline, the second reagent transfer pipeline, the 4th reagent transfer pipeline of the noting this moment flow of solution that do not have.
A)----" gathering again "----" startup is gathered " waited the execution of order to be analyzed if the normal delivered solution of energy is clicked " stopping collection ";
B) in the first reagent transfer pipeline, the 3rd reagent transfer pipeline if any one the road or the abnormal situation of two-way pipeline transportation solution, again peristaltic pump pressure pipe screw is compressed, until the normal liquor charging of energy,----" gathering again "----" starts and gathers ", waits the execution of order to be analyzed " to stop collection ";
C) " start and gather " clicked in the acquisition of typical curve, allows software move automatically after a complete analysis is finished, " stopping to gather ".In the process of bioassay standard curve, first three ten minutes, the uranin standard model of difference splendid attire variable concentrations in the 5th sampling receptacle 51,51,53; After 30 minutes, the standard model of the uranin of splendid attire in the 5th sampling receptacle 51,51,53 need be replaced by the standard solution of the different phenol of concentration.In the process that software moves automatically, solenoid valve can automatically switch to the different standard model of concentration in per ten minutes.At this moment the chemiluminescence that is produced by reaction is gathered by photomultiplier, amplify by high resistance operating amplifier then, the demodulation of high frequency modulated detuner, the filtering of signal processing circuit wave absorption, through analog to digital converter signal is changed into digital signal again, the programmed acquisition of last digital signal by compiling voluntarily, write down, store into industrial computer.Chemiluminescence Apparatus program in the industrial computer can the graphic meter operational process; With per ten minutes was a sampling period, within these ten minutes, get the mean value of the 8th minute the voltage in second minute road, mean value with voltage is independent variable, concentration with standard model is dependent variable, calculate the voltage V of measured object and the linear equation c=kV+b between the concentration c, the slope k and the intercept b that will calculate gained respectively are input in the chemiluminescence program relevant parameters position, carry out the analysis of actual water body example again.
D) " start and gather " opened in the actual sample analysis, allows software move automatically after a complete analysis is finished, and " stopping sampling " enters the state that standby is analyzed again.Software can calculate the content of measured object in the sample automatically, and the result is shown on the interface of chemiluminescence program.
E) will be replaced by pure water in first reagent container 11, second reagent container 22, the 3rd reagent container 33, first reagent container 44 again after finishing sample analysis and finishing, the first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline after using are cleaned.
F) behind two days the sample of the every test of cleaning, to be replaced by the oxalic acid washing lotion in first reagent container 11, second reagent container 22, the 3rd reagent container 33, first reagent container 44, the first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline after using are cleaned.
Claims (2)
1, a kind of chemiluminescence detector that is used for the online prison of water body example pollutant/detection comprises: FLUID TRANSPORTATION part (I), chemiluminescence prison/test section (II) and industrial computer control section (III);
Described FLUID TRANSPORTATION part (I) comprising: reagent transport portion (I
1) and tested water body sample delivery part (I
2);
Described reagent transport portion (I
1) comprising: the first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline and n reagent transfer pipeline that two ends link to each other with discharge pump (6) with the container that different reagent are housed respectively, n=2-10; The first reagent transfer solenoid valve (1), the second reagent transfer solenoid valve (2), the 3rd reagent transfer solenoid valve (3), the 4th reagent transfer solenoid valve (4) and n reagent transfer solenoid valve that the described reagent of control is carried are housed respectively on the described first reagent transfer pipeline, the second reagent transfer pipeline, the 3rd reagent transfer pipeline, the 4th reagent transfer pipeline and the n reagent transfer pipeline;
Described tested water body sample delivery part (I
2) comprising: the tested water body sample delivery pipeline that two ends link to each other with peristaltic pump (6) with the container that the tested water body sample is housed respectively, be equipped with the 5th tested water body sample delivery solenoid valve of this tested water body sample delivery of control on the described tested water body sample delivery pipeline;
Described chemiluminescence monitoring/test section (II) comprising: a circulation chamber chip (7) and a photomultiplier that links to each other with power supply (8), described power supply (81) operating voltage by city's alternating current transformer be converted to-700～-1100V; The photosensitive window of described photomultiplier (8) is relative with the helical duct of flow cell chip (7); Flow cell chip (7) is by first substrate that is carved with helical duct, second substrate that is carved with two parallel beeline channels and three layers of bonding chip that channel-less cover plate is formed;
After described first reagent transfer pipeline that is connected with peristaltic pump (6) output terminal and the 3rd reagent transfer pipeline converge, be connected with first inlet (a) of flow cell chip (7) again; After the described second reagent transfer pipeline that is connected with peristaltic pump (6) output terminal, the 4th reagent transfer pipeline mix by the glass three-way pipe, converge the back with the 5th tested water body sample delivery pipeline again and be connected with second inlet (b) of flow cell chip (7); After the mixed liquor that enters the reagent of described first inlet (a) and enter second inlet (b) mixes, use waste liquid outlet (c) discharge the helical flow path of flow cell chip (7) in;
Described industrial computer control section (III) comprising: control the described first reagent transfer solenoid valve (1), the second reagent transfer solenoid valve (2), the 3rd reagent transfer solenoid valve (3), the first control circuit (10) that the 4th reagent transfer solenoid valve (4) and n reagent transfer solenoid valve are opened or closed, control the second control circuit (20) that the 5th tested water body sample delivery solenoid valve opens or cuts out, high resistance operating amplifier (30), high frequency modulated detuner (40), signal processor (50), sixteen bit analog to digital converter (60) and industrial computer (70);
Described photomultiplier (8) output terminal, first control circuit (10) output terminal and second control circuit (20) output terminal link to each other with industrial computer (70) output terminal respectively; Described signal processor (50) links to each other with industrial computer (70) input end by sixteen bit analog to digital converter (60).
2, by the described chemiluminescence detector that is used for the online prison of water body example pollutant/detection of claim 1, it is characterized in that, also comprise a monoblock type outside framework, the inner space that constitutes three layers of cross direction profiles by two-layer bulkhead bracket that is provided with of described monoblock type outside framework; Described industrial computer control section (III) places the superiors; Chemiluminescence prison/test section (II) places the middle layer of complete machine; Described FLUID TRANSPORTATION part (I) places bottom.
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| CNB2005100865144A CN100504359C (en) | 2005-09-23 | 2005-09-23 | On-line monitoring chemical light-illuminating detector for contaminant in aquatic sample |
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|---|---|---|---|
| CNB2005100865144A CN100504359C (en) | 2005-09-23 | 2005-09-23 | On-line monitoring chemical light-illuminating detector for contaminant in aquatic sample |
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| CN100504359C CN100504359C (en) | 2009-06-24 |
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| CN101782523A (en) * | 2010-03-03 | 2010-07-21 | 宇星科技发展(深圳)有限公司 | Color developing agent for detecting arsenic in water quality online analyzer |
| CN101936972A (en) * | 2010-08-06 | 2011-01-05 | 上海理工大学 | Detector for glycated hemoglobin |
| CN101556249B (en) * | 2009-05-07 | 2011-08-31 | 上海交通大学 | Device for acquiring and processing signal of toxicity detector by bioluminescent method |
| CN102834717A (en) * | 2010-04-07 | 2012-12-19 | 英派尔科技开发有限公司 | Detector for detecting contaminants in water or aqueous solutions |
| CN105699307A (en) * | 2016-04-06 | 2016-06-22 | 芜湖英帕特工程技术有限公司 | Integrated experimental device and control method thereof |
| CN111443081A (en) * | 2019-01-17 | 2020-07-24 | 大韩民国(国立水产科学院) | Ambient pressure-induced substance measuring device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5300441A (en) * | 1989-02-11 | 1994-04-05 | Antek Instruments, Inc. | Method for measuring and determining nitrogen content in a sample and providing an output data of total nitrogen and selected constituent nitrogen compounds including nitrate and nitrite |
| CN1116303A (en) * | 1994-08-31 | 1996-02-07 | 陈义新 | Automatic measurer for content of silicic acid group |
| CN2549458Y (en) * | 2002-07-01 | 2003-05-07 | 中国科学院生态环境研究中心 | Fluid injected chemiluminescent passing pool chip for chemiluminescence analysis |
| CN1266467C (en) * | 2004-03-10 | 2006-07-26 | 沈阳七彩科技工程有限公司 | On-line automatic analizer for urban saprobia toxic and its using method |
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2005
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101556249B (en) * | 2009-05-07 | 2011-08-31 | 上海交通大学 | Device for acquiring and processing signal of toxicity detector by bioluminescent method |
| CN101782523A (en) * | 2010-03-03 | 2010-07-21 | 宇星科技发展(深圳)有限公司 | Color developing agent for detecting arsenic in water quality online analyzer |
| CN102834717A (en) * | 2010-04-07 | 2012-12-19 | 英派尔科技开发有限公司 | Detector for detecting contaminants in water or aqueous solutions |
| CN102834717B (en) * | 2010-04-07 | 2015-05-27 | 英派尔科技开发有限公司 | Detector for detecting contaminants in water or aqueous solutions |
| CN101936972A (en) * | 2010-08-06 | 2011-01-05 | 上海理工大学 | Detector for glycated hemoglobin |
| CN105699307A (en) * | 2016-04-06 | 2016-06-22 | 芜湖英帕特工程技术有限公司 | Integrated experimental device and control method thereof |
| CN111443081A (en) * | 2019-01-17 | 2020-07-24 | 大韩民国(国立水产科学院) | Ambient pressure-induced substance measuring device |
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