CN1924017A - Crested wheatgrass P genome specific sequence - Google Patents
Crested wheatgrass P genome specific sequence Download PDFInfo
- Publication number
- CN1924017A CN1924017A CN 200610113147 CN200610113147A CN1924017A CN 1924017 A CN1924017 A CN 1924017A CN 200610113147 CN200610113147 CN 200610113147 CN 200610113147 A CN200610113147 A CN 200610113147A CN 1924017 A CN1924017 A CN 1924017A
- Authority
- CN
- China
- Prior art keywords
- sequence
- wheatgrass
- wheat
- genome
- genome specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
the invention discloses an astopic sequence of crested wheatgrass P gene group, which is characterized by the following: adopting gene group DNA as mould to do PCR augmentation for RAPD primer; obtaining 1036bp nucleotide sequence; or using SCAR primer to do PCR augmentation to obtain 960bp nucleotide sequence; possessing a couple of oligonucleotide with sequence 3 and sequence 4 for SCAR primer; providing new astopic probe and molecular mark for P chromatin rapidly and exactly.
Description
Technical field
The present invention relates to crested wheatgrass P genome specific sequence.
Background technology
Wheat is human staple food crop, occupies critical role in agriculture production.At present, narrow wheat improvement is caused serious hindrance, wheat wild kindred plant is the huge gene pool of genetic improvement of wheat in the cultivated wheat hereditary basis.
There are 25 genus in wheat family, and nearly 400 kinds are occupied critical role in grass.The basic genome of Agropyron (Agropyron) plant is P group (Dong Yuchen, Zheng Diansheng.China's wheat genetic resource.Chinese agriculture press, 2000,174-177), be distributed in cool temperature zone plateau, Eurasia and sand ground, not only long-term adaptation arid, the abiotic stress that cold climate and soil depletion caused, and main disease such as the Powdery Mildew of wheat, yellow dwart, rust had very strong resistance, in addition, wheatgrass has high yield plant type structures such as many small ears, many Xiao Hua.Therefore, wheatgrass P genome can be used as multiple excellent foreign gene donor, and is significant for genetic improvement of wheat.
(Li Lihui such as Li Lihui, Li Xiuquan, Li Pei. the establishment of wheat-wheatgrass alien addition line. Acta Genetica Sinica, 1997,24:154-159) breed the wheat-wheatgrass allos disomic addition line of a cover genetic stability in the world first, and obtained the confirmation of molecular cytogenetics.Heredity exchange between wheat ABD group and wheatgrass P group is created in being established as of wheat-wheatgrass addition line, and the excellent transgenosis that P karyomit(e) is carried provides important prerequisite in wheat.By the hybridization of wheat and wheatgrass addition line, obtained the higher wheat of a collection of using value-wheatgrass recombination system, very necessary to the chromatinic Genetic identification of P in the recombination system.
Genomic in situ hybridization (Genomic in situhybridization, method GISH), but this is often more effective for the evaluation of heterochromosome replacement and big fragment transposition are adopted in the heterochromatic evaluation of wheat external source usually.Because chromosome substitution and big fragment transposition may cause chain burden when importing excellent gene, so people more wish to obtain the transposition of heterochromatin small segment.Yet the evaluation difficulty for the transposition of heterochromatin small segment is a stubborn problem always.Along with the development and the application of Protocols in Molecular Biology, utilize genome high abundance distinguished sequence to be probe, further set up identification different genes group and chromosomal specific molecular marker, for new approach has been opened up in heterochromatic evaluation.Wheat-wheatgrass recombination system heterochromatin small segment transposition is carried out Genetic identification fast and accurately, pressed for chromosomal specific probe of P genome and P and molecule marker.
The research of wheat family plant different genes group distinguished sequence receives much attention always, the relation because the heredity that exists between the wheat family different genes group is developed, and genome specific sequence is for other genomes.The ideal distinguished sequence has specificity with respect to other most genomes.At present in the wheat kindred plant, clone the genomic distinguished sequence of R (Mao L from rye, Zhai WX, Hu H.Cloning and characterization of arepetitive sequence in rye (Secale cereal) .Plant Science, 1994,100:51-57; Zhou Jianping, Yang Zujun, Feng Juan. the separation and the evaluation of rye specific DNA tumor-necrosis factor glycoproteins. southwestern agriculture journal .2005,18 (5): 598-602); Clone the genomic distinguished sequence of H (Hueros G from barley, Loarce Y, FerreyE.A structure and evolutionary analysis of a dispersed repetitivesequence.Plant Mol Biol.1993,22:635-643; Anamthawat-Jonsson K, Heslop-harrison JS.Isolation and characterization of genome specific DNAsequences in Trticease species. Mol Gen Genet, 1993,240:151-158), set up the specific molecular marker (Huang Chaofeng of barley chromosome, Zhang Wenjun, the repercussions billows. the screening and the evaluation of barley 6H chromosome specific mark. Acta Genetica Sinica .2000,27 (8): 713-718); From long fringe couchgrass clone the genomic special tumor-necrosis factor glycoproteins of E (Li Hongjie. the separation of long fringe couchgrass (Thinopyrum elongatum) genome specific tumor-necrosis factor glycoproteins. Chinese Academy of Sciences's post-doctoral research work report, 2000); Clone the St genome specific sequence and set up the special PCR mark (Zhang Zengyan of 2Ai-2 karyomit(e) from middle couchgrass, Wang Lili, Xin Zhiyong. the foundation of the new mark of middle couchgrass karyomit(e) 2Ai-2 specific PCR and the clone of St genome specific sequence. Acta Genetica Sinica .2002,29 (7): 565-570); Clone the genomic distinguished sequence of D (Lagudahe S from aegilops tauschii, Appels R, Mcneild.The Nor-D3 of Triticum tauschii:Natural variation andgenetic linicage to markers in chromosome 5.Genome, 1991b, 34:387-395); Clone the genomic distinguished sequence of V (DePace C from cluster hair wheat, Deler V, Scarasciia MGT.Molecular and chromosomal characterization of repeated and single copy DNAsequences in the genome of Dasypyrum villosum.Hereditas, 1992,116:55-65; Li W L, Chen P D, Qi L L.Isolation, characterization and application of aspecies-specific repeated sequence from Haynaldia villosa.Theor Appl Genet, 1995,90:526-533), set up the genomic specific PCR mark (Liu Shoubin of cluster hair wheat V, the sunshine Tang Dynasty, You Mingshan. the foundation and the application of cluster hair wheat genome specificity PCR mark. Acta Genetica Sinica .2003,30 (4): 350-356); Clone the genomic distinguished sequence of C (Baldauf F from the shape of tail goatweed, Schubert V, Metzlaff M.Repeated DNA sequences of Aefilops markgra-fii (Geuter) Hammervar, markgrafii:cloning, sequencing and analysis of distribu-tion inPoaceaae species.Hereditas, 1992,116:71-78; Kong Xiuying, Zhou Ronghua, Dong Yuchen.The clone of shape of tail goatweed C genome specific tumor-necrosis factor glycoproteins.Science Bulletin, 1999a, 44 (8): 828-832); From intend this inferior you take off goatweed and clone the genomic distinguished sequence of S (Daud H M, and Gustafson J P.Molecular evidence for Triticum speltoides as a B-genome progenitor of wheat (Triticum aestivum) .Genome, 1996,39:543-548; Giorgi D, D ' Ovidio R, Tanzarella O A.Isolation and characterization of S genome specific sequencesfrom Aegilops sect.sitopis species.Genome, 2003,46:478-489); From rely grass clone the genomic distinguished sequence of Ns (Sigridur K B, and Kesara A.Isolation, characterization, and analysis of Leymus-specific DNA sequences.Genome, 2003,46:673-682).
Genome specific dna sequence dna and Study on Molecular Marker not only provide theoretical foundation for illustrating the botanical system evolution of wheat family, composition of genome and genome evolution relation, and provide the specific probe and molecule marker (the Orgaard M that can directly utilize for the chromatinic evaluation of wheat external source, Anamthawat-Jonsson K.Genomediscrimination by in situ hybridization in Icelandic species of Elymus andElytrigia (Poaceae:Triticeae) .Genome.2001,44:275-283; SchoenenbergerN, Felber F, Savova-Bianchi D.Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrical Host * Triticum aestivum L.hybrids.Theor Appl Genet.2005,111:1338-1346).
Yet, do not see special report as yet for the research of Agropyron P genome specific sequence and the chromosomal specific molecular marker of identification P.
Summary of the invention
The purpose of this invention is to provide a kind of crested wheatgrass P genome specific sequence.
Crested wheatgrass P genome specific sequence provided by the present invention is that the genomic dna with wheatgrass is a template, carries out the nucleotide sequence of the 1036bp that pcr amplification obtains with the RAPD primer, or carries out the nucleotide sequence of the 960bp that pcr amplification obtains with the SCAR primer; The nucleotide sequence of described RAPD primer is a sequence 1, a pair of oligonucleotide that described SCAR primer is made up of sequence 3 and sequence 4.
In actual applications, the template of described PCR can be the genomic dna of wheatgrass Z559, the genomic dna of wheatgrass Z804 or the genomic dna of wheatgrass Z1750.
Described crested wheatgrass P genome specific sequence, by sequence in the sequence table 2 from 5 ' the 75th sequence or sequence of forming to the 1034th deoxynucleotide in the sequence table 2 of end.
Because Agropyron P karyomit(e) exists heredity to reset phenomenon, bring difficulty for the research of the genomic evolution relation of P, the P genome specific sequence that the present invention obtains will provide new molecular biology foundation for illustrating the genomic evolution relation of wheat family phylogeny of plants and Agropyron plant P.The chromatinic detection of wheat external source P is a key of identifying wheat-wheatgrass recombination system, the a large amount of individual plant evaluation work of filial generation is very numerous and diverse, the P genome specific sequence of the present invention's screening and the P genome SCAR mark that obtains based on this sequence will provide new specific probe and molecule marker for wheat-chromatinic rapid detection of wheatgrass recombination system P and accurate the evaluation.
Description of drawings
Fig. 1 is the pcr amplification result of primer Opx-7 to Agropyron P genomic dna and the ABD of wheat family genomic dna
Fig. 2 is the pcr amplification result of SCAROPX-7 primer to wheatgrass and other different genes groups DNA of wheat family
Embodiment
Following experimental technique is ordinary method if no special instructions.
The other plant of the wheatgrass among the following embodiment, wheat and wheat family is all available from Institute of Crop Science, Chinese Academy of Agricultural Science germ plasm resource research centre.
The acquisition of embodiment 1, P genome specific sequence
Utilize RAPD primer Opx-7:5 '-GAGCGAGGCT-3 ' (sequence 1), Agropyron P genomic dna and the ABD of wheat family genomic dna are screened, separate and clone P genome specific amplified fragments.
Wherein, the 20ul system of pcr amplification: 10 * PCRbuffer (contains 20mMol/L Mg
2+) 2ul
dNTP(10mM) 0.3ul
Primer (8uM) 0.7ul
Taq enzyme (2u/ul) 0.5ul
Template DNA (50ng/ul) 1ul
ddH
2O 15.5ul
PCR program: 94 ℃ of abundant sex change of 4min earlier; 94 ℃ of 30S sex change again, 37 ℃ of 45S renaturation, 72 ℃ of 1min extend, 45 circulations; Last 72 ℃ are extended 10min.4 ℃ of preservations.
1.2% sepharose, 100V constant voltage electrophoresis, ultraviolet gel imaging.
The pcr amplification result amplifies the special band that the ABD of wheat family genome does not have as shown in Figure 1 in diploid, tetraploid and hexaploid wheatgrass P genome, this stripe size is 1036bp.Among Fig. 1, L is 100bp DNA Ladder; P2, diploid wheatgrass Z804 (PP); P4, tetraploid wheatgrass Z559 (PPPP); P6, hexaploid wheatgrass Z1750 (PPPPPP); CS, common wheat China spring (AABBDD); Fu, common wheat Fukuho (AABBDD); A, einkorn wheat MO4 (AA); AB, durum wheat DR4 (AABB);-, blank (pure water); Arrow is depicted as P genome specific amplified band.
Cut the P genome specific DNA band of diploid wheatgrass Z804, tetraploid wheatgrass Z559 and hexaploid wheatgrass Z1750, and reclaim test kit Agarose Gel DNA Purification (precious biotechnology Dalian company limited) with DNA target DNA is reclaimed.Specific fragment is after reclaiming, use T-support agent box (precious biotechnology Dalian company limited) to connect through pMD-18 Vector, connecting product is converted in the F-strain e. coli jm109, through blue hickie screening, the picking white colony extracts plasmid DNA, cut evaluation through pcr amplification and enzyme, determine to insert the clip size clone consistent check order (checking order) by Beijing an ancient unit of weight Yao great achievement biotech company with former recovery fragment.Sequencing result shows that the specific fragment length that increases is 1036bp from tetraploid wheatgrass Z559, have the nucleotide sequence of sequence 2 in the sequence table; The specific fragment length that increases from diploid wheatgrass Z804 is 1036bp, has the nucleotide sequence of sequence 2 in the sequence table; The specific fragment length that increases from hexaploid wheatgrass Z1750 is 1036bp, has the nucleotide sequence of sequence 2 in the sequence table.
Embodiment 2, crested wheatgrass P genome specific sequence checking
According to the sequencing result of P genome specific amplified fragments, optimize through the DNASTAR design software, design a pair of SCAROPX-7 primer: SCAROPX-7F:5 '-GCTACCATTCCAACTTCTACG-3 ' (sequence 3), corresponding to the 75-95nt of sequence 2; SCAROPX-7R:5 '-GCGAGGCTATGTTGGTTTTGA-3 ' (sequence 4) is with the 1014-1034nt reverse complemental of sequence 2.
With the SCAROPX-7 primer different genes group DNA of wheat family is increased (62 ℃ of annealing temperatures), amplification and deposition condition are with embodiment 1.The result shows, at diploid wheatgrass Z804, the length that all increases among tetraploid wheatgrass Z559 and the hexaploid wheatgrass Z1750 is the specific fragment (SCAR mark) of 960bp, and at wheat ABD genome and most wheat other genomes (C of family, D, E, AG, I, R.S.St, U, V, W does not obtain the specific fragment of this 960bp in Y), illustrates that the sequence that increases from the genomic dna of wheatgrass with the SCAROPX-7 primer is to wheat ABD genome and most wheat other genomes (C of family, D, E, AG, I, R.S.St, U, V, W, Y) performance specificity (Fig. 2) is a crested wheatgrass P genome specific sequence.Among Fig. 2, L is 100bp DNA Ladder; P2 is diploid wheatgrass Z804 (PP); P4 is tetraploid wheatgrass Z559 (PPPP); P6 is hexaploid wheatgrass Z1750 (PPPPPP); CS is common wheat China spring (AABBDD); Fu is common wheat Fukuho (AABBDD); A is einkorn wheat MO4 (AA); AB is durum wheat DR4 (AABB); C is shape of tail goatweed Ae14 (CC); D is aegilops tauschii Y93 (DD); E is a long fringe couchgrass (EE); AG is timopheevi wheat TI
1(AAGG); H is purple Herba Hordei Vulgaris Z2826 (HH); I is purple six ribs cultivation barley (II); M is tip awn goatweed Y258 (MM); Ns is new wheat straw R429 (NsNs); R is rye Z1383 (RR); S takes off goatweed Ae49 (SS) for intending this inferior that; St is false roegneria kamoji (StSt); U is umbellule goatweed Y39 (UU); V is cluster hair wheat Z1731 (VV); W is Australia wheat straw (WW); Y is middle roegneria kamoji (StStYY);-be blank (pure water).
Above-mentioned SCAR mark is checked order.Sequencing result shows that the specific fragment length that increases is 960bp from tetraploid wheatgrass Z559, by sequence in the sequence table 2 from 5 ' the 75th to the 1034th sequence that deoxynucleotide is formed of end; The specific fragment length that increases from diploid wheatgrass Z804 is 960bp, by sequence in the sequence table 2 from 5 ' the 75th to the 1034th sequence that deoxynucleotide is formed of end; The specific fragment length that increases from hexaploid wheatgrass Z1750 is 960bp, by sequence in the sequence table 2 from 5 ' the 75th to the 1034th sequence that deoxynucleotide is formed of end.
Sequence table
<160>4
<210>1
<211>10
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>1
gagcgaggct 10
<210>2
<211>1036
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>2
gagcgaggct?cgtgagcaca?acacggaggt?tgaggctact?catgcggagg?cggctgcacc 60
aagggcctcc?gtgagctacc?attccaactt?ctacgcagat?cccggatggt?gaattccaag 120
ctaggcaaaa?gccaaagctt?gggggagtac?gtgtttctca?ccgacattac?attcatgttc 180
acacatttca?tttcagttgt?cggtgctcac?acgtttccat?tgcattatcc?ttgctagaat 240
tattttcttg?ctctcttttt?gtgtgtttga?aaaactttca?gaaaactcaa?aaatatttcc 300
ttgcttcttt?ttggcttttt?cttctaagtt?tagttagtct?caattaagag?aaaacccaaa 360
aagatttcct?tgttcttctt?ttgctttttg?ggagctttcc?cgtgtaaata?gttttcttgt 420
ttttgttttt?cctttttttt?aatttgcttt?gcttttcaaa?aaactaaaaa?ctccaaaaat 480
atttccagtg?tgtttctatg?aatttctttt?tcttttttgt?tgagtcttag?taatgagagg 540
accacgatga?aatgatgagt?ggcactgata?tgcattgatg?tttatctaat?gagacaagtt 600
attatgtctt?ctcttggtcg?aataaaatgt?tttgcagatt?ccagcctagt?ccacagtaca 660
cttgaactta?tatctttata?tcattcggtc?gtgcaagtga?aaggcaataa?tgacgatgac 720
ttgatgaagt?gactgtgtca?aagaaaagct?ggtatgaact?caacttgttt?tttgtttttg 780
taaatatgtt?tatcttagta?gccatgactt?agcatgatta?tgatcaaaca?tgtttatgat 840
gacaattaga?gattatagtt?gctcatgcca?tgcttaatag?ctaggagtgg?ataatgattt 900
atcttgaatg?ttaacatgca?ttagaatgat?tgtgatgtag?tatgatgaca?tggtatcttc 960
ctctgaatgt?ttcaagtggc?ttgacttggc?acatgttcac?gcatgtagtt?gaatcaaaac 1020
caacatagcc?tcgctc 1036
<210>3
<211>21
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>3
gctaccattc?caacttctac?g 21
<210>4
<211>21
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>4
gcgaggctat?gttggttttg?a 21
Claims (6)
1, a kind of crested wheatgrass P genome specific sequence is that the genomic dna with wheatgrass is a template, carries out the nucleotide sequence of the 1036bp that pcr amplification obtains with the RAPD primer, or carries out the nucleotide sequence of the 960bp that pcr amplification obtains with the SCAR primer; The nucleotide sequence of described RAPD primer is a sequence 1, a pair of oligonucleotide that described SCAR primer is made up of sequence 3 and sequence 4.
2, crested wheatgrass P genome specific sequence according to claim 1 is characterized in that: the template of described PCR is the genomic dna of wheatgrass Z559.
3, crested wheatgrass P genome specific sequence according to claim 1 is characterized in that: the template of described PCR is the genomic dna of wheatgrass Z804.
4, crested wheatgrass P genome specific sequence according to claim 1 is characterized in that: the template of described PCR is the genomic dna of wheatgrass Z1750.
5, according to arbitrary described crested wheatgrass P genome specific sequence in the claim 2 to 4, it is characterized in that: described crested wheatgrass P genome specific sequence, by sequence in the sequence table 2 from 5 ' the 75th to the 1034th sequence that deoxynucleotide is formed of end.
6, according to arbitrary described crested wheatgrass P genome specific sequence in the claim 2 to 4, it is characterized in that: described crested wheatgrass P genome specific sequence is the sequence 2 in the sequence table.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610113147 CN100526464C (en) | 2006-09-15 | 2006-09-15 | Crested wheatgrass P genome specific sequence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610113147 CN100526464C (en) | 2006-09-15 | 2006-09-15 | Crested wheatgrass P genome specific sequence |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1924017A true CN1924017A (en) | 2007-03-07 |
| CN100526464C CN100526464C (en) | 2009-08-12 |
Family
ID=37816842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610113147 Expired - Fee Related CN100526464C (en) | 2006-09-15 | 2006-09-15 | Crested wheatgrass P genome specific sequence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100526464C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243164A (en) * | 2013-05-06 | 2013-08-14 | 西北农林科技大学 | Method for identifying psathyrostachys huashanica allogenic material in wheat genome |
| CN105331605A (en) * | 2014-08-07 | 2016-02-17 | 中国农业科学院作物科学研究所 | P genome specific Gypsy retrotransposon and application thereof |
| CN105368821A (en) * | 2014-08-07 | 2016-03-02 | 中国农业科学院作物科学研究所 | P genome acrocentric region specific Copia retrotransposon and applications thereof |
| CN105441428A (en) * | 2014-08-07 | 2016-03-30 | 中国农业科学院作物科学研究所 | Agropyron cristatum centromere specific Gypsy retrotransposon sequence and application thereof |
| CN105441427A (en) * | 2014-08-07 | 2016-03-30 | 中国农业科学院作物科学研究所 | Agropyron cristatum chromosome end portion specific tandem repeat sequence and application thereof |
| CN110055317A (en) * | 2019-04-29 | 2019-07-26 | 中国农业科学院作物科学研究所 | The method of external source function candidate gene is excavated in wheat distance edge hybrid offspring's Small piece transposition system |
| CN112280889A (en) * | 2020-11-10 | 2021-01-29 | 中国农业科学院作物科学研究所 | Specific oligonucleotide probe for wheatgrass chromosome and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1450786A1 (en) * | 1986-05-08 | 1989-01-15 | Всесоюзный Научно-Исследовательский Институт Прикладной Молекулярной Биологии И Генетики | Method of determining perennial life of hybrid cereal crops based on agropyron |
-
2006
- 2006-09-15 CN CN 200610113147 patent/CN100526464C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243164A (en) * | 2013-05-06 | 2013-08-14 | 西北农林科技大学 | Method for identifying psathyrostachys huashanica allogenic material in wheat genome |
| CN105331605A (en) * | 2014-08-07 | 2016-02-17 | 中国农业科学院作物科学研究所 | P genome specific Gypsy retrotransposon and application thereof |
| CN105368821A (en) * | 2014-08-07 | 2016-03-02 | 中国农业科学院作物科学研究所 | P genome acrocentric region specific Copia retrotransposon and applications thereof |
| CN105441428A (en) * | 2014-08-07 | 2016-03-30 | 中国农业科学院作物科学研究所 | Agropyron cristatum centromere specific Gypsy retrotransposon sequence and application thereof |
| CN105441427A (en) * | 2014-08-07 | 2016-03-30 | 中国农业科学院作物科学研究所 | Agropyron cristatum chromosome end portion specific tandem repeat sequence and application thereof |
| CN105368821B (en) * | 2014-08-07 | 2017-07-21 | 中国农业科学院作物科学研究所 | A kind of special Copia retrotransponsonses of nearly centric region of P genomes and its application |
| CN105441428B (en) * | 2014-08-07 | 2017-07-21 | 中国农业科学院作物科学研究所 | A kind of special Gypsy retrotransponsonses sequence in wheatgrass centromere and its application |
| CN105331605B (en) * | 2014-08-07 | 2017-07-25 | 中国农业科学院作物科学研究所 | A kind of Gypsy retrotransponsonses of P genome specifics and its application |
| CN110055317A (en) * | 2019-04-29 | 2019-07-26 | 中国农业科学院作物科学研究所 | The method of external source function candidate gene is excavated in wheat distance edge hybrid offspring's Small piece transposition system |
| CN110055317B (en) * | 2019-04-29 | 2022-04-26 | 中国农业科学院作物科学研究所 | Method for discovering exogenous functional candidate genes in wheat distant hybridization progeny small-fragment translocation line |
| CN112280889A (en) * | 2020-11-10 | 2021-01-29 | 中国农业科学院作物科学研究所 | Specific oligonucleotide probe for wheatgrass chromosome and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100526464C (en) | 2009-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1924017A (en) | Crested wheatgrass P genome specific sequence | |
| Kalyana Babu et al. | In-silico mining, type and frequency analysis of genic microsatellites of finger millet (Eleusine coracana (L.) Gaertn.): a comparative genomic analysis of NBS–LRR regions of finger millet with rice | |
| CN104131012A (en) | Molecular marker for identifying soybean nucleus male sterility line and identification method thereof | |
| Zhu et al. | Development of genome-wide SSR markers in rapeseed by next generation sequencing | |
| Yang et al. | Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.) | |
| Bibi et al. | Analysis of genetic diversity in linseed using molecular markers | |
| Li et al. | Evaluation of population structure, genetic diversity and origin of Northeast Asia weedy rice based on simple sequence repeat markers | |
| CN1314816C (en) | Molecular mark of watermelon linked to gene resistant to field pumpkin yellowwatermelon yellow mosaic virus and uses | |
| Oo et al. | Analysis of the genetic diversity and population structure of amaranth accessions from South America using 14 SSR markers | |
| CN103695418A (en) | Maize phosphate starvation responses intron length polymorphism marker for corn | |
| Mohamed et al. | Genetic diversity of some Saudi barley (Hordeum vulgare L.) landraces based on microsatellite markers | |
| Liu et al. | Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder | |
| Riju et al. | Mining of expressed sequence tag libraries of cacao for microsatellite markers using five computational tools | |
| Su et al. | DNA microsatellite analysis of genetic diversity among Chinese indigenous laying-type ducks (Anas platyrhynchos) | |
| Manjunatha et al. | Genetic structure of hull-less barley (Hordeum vulgare L. subsp. vulgare) landrace populations from North-western Indian Himalayas | |
| CN101845499B (en) | Primer pair for assisting for screening wheat powdery mildew and its uses | |
| Fa et al. | Genetic differences in homoeologous group 1 of seven types of winter wheat Aimengniu | |
| Li et al. | Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa | |
| Anuarbek et al. | Validation of bread wheat KASP markers in durum wheat lines in Kazakhstan | |
| YANG et al. | Sequence polymorphisms and phylogenetic relationships of hina Gene in wild barley from Tibet, China | |
| Chen et al. | Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus | |
| CN111826455B (en) | Molecular marker VrMLO _ Indel1 for identifying powdery mildew resistance phenotype of mung beans as well as primer and application thereof | |
| CN111826456B (en) | Molecular marker SNP #1 for identifying powdery mildew resistance phenotype of mung bean, and primer and application thereof | |
| Ou et al. | Chloroplast DNA polymorphism in different types of cytoplasmic male sterile rice | |
| De Silva et al. | Polymorphic SSR markers among Oryza sativa cv. Swarna and its derived advanced backcross lines with wild introgressions from O. nivara |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090812 Termination date: 20120915 |