CN1921948A - Separation apparatus and method - Google Patents

Separation apparatus and method Download PDF

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Publication number
CN1921948A
CN1921948A CNA2004800396357A CN200480039635A CN1921948A CN 1921948 A CN1921948 A CN 1921948A CN A2004800396357 A CNA2004800396357 A CN A2004800396357A CN 200480039635 A CN200480039635 A CN 200480039635A CN 1921948 A CN1921948 A CN 1921948A
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China
Prior art keywords
split tunnel
dividing plate
fluid
blood
flow path
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Granted
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CNA2004800396357A
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Chinese (zh)
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CN1921948B (en
Inventor
闵庚伦
理查德·I·布朗
阿尔普·阿科努尔
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Fenwal Inc
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Baxter International Inc
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Priority claimed from US10/827,603 external-priority patent/US7297272B2/en
Application filed by Baxter International Inc filed Critical Baxter International Inc
Publication of CN1921948A publication Critical patent/CN1921948A/en
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Abstract

A separation apparatus and method are employed using a spearation channel (210) for rotation abut an axis. Such channel includes radially spaced apart inner (206) and outer (208) side wall portions and an end wall portion (224). An inlet (226) conveys fluid into the channel. A barrier (232) is located in the channel intermediate of the inner and outer side wall portions. A first flow path communicates between upstream and downstream sides of the barrier. A collection region may be located downstream of the barrier for communication with teh first flow path. An outer side wall section of the channel may be positioned radially outward of an upstream section thereof. The barrier (232) may joing the outer side wall portion (208) along a substantial portion of an axial length of the channel. First and second exit flow paths may allow communication with the channel either upstream or downstream of the barrier or both.

Description

Separation equipment and method
Related application
The application requires October in 2002 24 that submit to, names to be called the pending trial U.S. Patent Application Serial Number 10/279 of " being used for gathering; do not have or do not have basically the blood processing system and the method for the blood plasma of cell blood component ", 765 benefit of priority, and require the U.S. Provisional Patent Application sequence number 60/533 submitted on December 31st, 2003, No. 820 benefit of priority, these two applications are incorporated into all that this is for reference.
Technical field
The present invention relates generally to a kind of equipment and method that is used for the biofluid such as blood or blood constituent or other fluid is separated into one or more compositions.
Background technology
To be separated into its constituent such as the biofluid of whole blood or blood constituent, to be used for various application be known.Many commercial available piece-rate systems are based on centrifugal principle, and it is according to the density separation fluid composition.It is known adopting the various devices and the system of centrifugation blood or blood constituent, comprises CS-3000, Amicus and the Alyx separator sold by the Baxter Healthcare company in Illinois, USA Deerfield city; Spectra and Trima separator that the Gambro BCT in Lakewood city, Colorado sells; From the AS104 of the Fresenius Homecare in Washington state Redmond city, and from V-50 and other model of the Haemonetics company in Massachusetts, United States Braintree city.At United States Patent (USP) 6, multiple centrifugal device is also disclosed in 325, No. 775, disclosed International Application PCT/US02/31317 number, PCT/US02/31319 number, PCT/US03/33311 number and PCT/US03/07944 number and the laid-open U.S. Patents application No. 20020094927 and No. 20020077241.These patents and patent application are incorporated into that this is for reference.
Though centrifugal blood separating mechanism is well-known, develop less, gentlier, more portable, general and/or separate efficiently and the effort of device of other biofluid of gathering blood or one or more heterogeneities also in continuation.
Summary of the invention
The present invention includes and be used to separate such as the biofluid of whole blood and the apparatus and method of selectively gathering at least a fluid composition.
According to embodiments of the invention, be provided with and be used for the split tunnel that rotates around axis.This split tunnel comprises madial wall part and lateral wall part and the end wall portion that is radially spaced.This split tunnel has certain axial length with respect to axis.Be provided with inlet, in order to transporting fluid in this split tunnel, and this madial wall part and lateral wall passage interfix partly dividing plate.This dividing plate comprises upstream side and downstream, and comprises first flow path that is communicated with between this upstream side and the downstream.This split tunnel also comprises downstream that is positioned at this dividing plate and the acquisition zone that is communicated with this first mobile path flow body.This acquisition zone is at least in part by limiting with the axially spaced end wall branch of the end wall portion of this split tunnel.In addition, first opening and second opening are communicated with this acquisition zone, so that can flow from this acquisition zone such as one or more fluid compositions of whole blood.
In an embodiment of the present invention, the part of the lateral wall of this split tunnel part is positioned near this dividing plate, and is positioned at the radial outside of this lateral wall part at the upstream end of this part.
In another embodiment of split tunnel, this dividing plate extends to the lateral wall part, and connects this in the lateral wall part along the sizable a part of of axial length of this split tunnel.The first fluid path makes between the upstream side of this dividing plate and the downstream and can be communicated with by fluid.
In another embodiment of split tunnel, dividing plate can extend to the radial position that is positioned at inside radial position place of madial wall part.
Another embodiment of this split tunnel is included in first flow path that is communicated with between the upstream side of dividing plate and the downstream, and comprises the first outlet flow path and the second outlet flow path.This first outlet flow path is communicated with split tunnel in the upstream of this dividing plate, and the second outlet flow path is communicated with this split tunnel in the downstream of this dividing plate.This first flow path converges in a radially inner position of the madial wall part of this split tunnel mutually with second flow path.
In addition, another split tunnel can provide: a plurality of exit openings that this split tunnel is provided in the downstream of this dividing plate.In this respect, in the upstream of dividing plate, this split tunnel does not have exit opening, because do not allow fluid composition to leave this groove in the position of dividing plate upstream.The first fluid flow path allows to be communicated with between the upstream side and downstream of this dividing plate, but the outlet flow path that leads to this split tunnel outside is not provided.
In another embodiment of this split tunnel, baffle wall extends to the radial outside wall part of this split tunnel.First flow path is communicated with between the upstream side and downstream of this split tunnel, and spaced apart with one of relative end wall portion of this split tunnel.This split tunnel also comprises the upstream side that is communicated with this dividing plate and second flow path between the downstream, and this second flow path is limited by the surface institute of another end wall portion.
Though describe according to some preferred embodiments below, should be appreciated that the same spline structure of split tunnel of the present invention shown in being not limited to.For example, split tunnel can comprise that disposable flexibility, rigidity or semirigid liner are arranged on reusable cylinder, floating drum or rotor wherein, so that blood flow is crossed this liner and do not contacted this reusable part.In this case, the structure of the cylinder of this split tunnel, floating drum or rotor defines the shape of fluid flow path, and during operation, this disposable pad presents corresponding shape.This example can be referring to CS-3000, Amicus and Spectra centrifugal separation system.Perhaps, split tunnel can be disposable fully.For example, this split tunnel can the rigidity plastics of shaping form forms by having in advance, by its processing blood or other biofluid.Certainly, this split tunnel can be reusable fully, in this case, need clean and possible sterilization between using, and this is inconvenient and consuming time.Should be appreciated that described and the split tunnel of being advocated and method are intended to have and comprise all extensive interpretations with all concrete structures of commercial use as mentioned above.
Split tunnel as described herein or chamber can be used for various biofluids and separate and gatherer process.By means of giving an example rather than restrictive mode, one of this separation method comprises the steps: and will introduce in the centrifugal field such as the first fluid of whole blood, this first fluid comprises at least the first composition and second composition, for example, usually the blood constituent that has different densities, and can be at this first composition and second composition form interface at least between the several portions.This method may further comprise the steps: remove (second fluid, and remove the 3rd fluid from this interfacial opposite side from this interfacial side; At least a portion second fluid and first fluid or the 3rd combination of fluids are got up, and this fluid that combines is introduced in this centrifugal field again; And from this centrifugal field, remove this second fluid or the 3rd fluid at least one of them.
When said method being applied to whole blood (first fluid), this second fluid can mainly comprise blood plasma, and the 3rd fluid can mainly comprise red blood cell.Second fluid that this combines and first fluid or the 3rd fluid can have the hematocrit between about percent 20 to percent 40.That part of of blood plasma of removing from this interface one side can comprise considerable blood platelet.
According to another kind of method of the present invention, this method may further comprise the steps: will introduce in the centrifugal field such as the first fluid of whole blood, this first fluid comprises first composition and second composition of the different densities that has usually; Permission forms interface at least this first fluid composition and second fluid composition between the several portions; Reduce the power (for example rotary area of the separation chamber by reducing to comprise this fluid) of centrifugal field; And after reducing the power of centrifugal field, from this centrifugal field, remove the first fluid composition.
Can repeat some or all in the above-mentioned steps of this method, to strengthen efficient.For example, can repeat to remove the step of fluid composition, collect circulation several times thereby provide.Said method can have special purposes when gathering blood platelet from whole blood, wherein the first fluid composition comprises blood plasma, and this blood plasma comprises blood platelet.
Another kind of method of the present invention provides and formed and formed again interface between the fluid composition of different densities.This method comprises the steps: to introduce the first fluid such as the whole blood of first composition with different densities and second composition; Permission is forming interface at least such as the blood plasma of whole blood and the first fluid between the red blood cell and second fluid between the several portions; From this interfacial side, sequentially and repeatedly from centrifugal field, remove fluid, and allow interface to form again.
When this method was applied to whole blood, the fluid of removing from centrifugal field comprised blood plasma and blood platelet.Specifically, this fluid can comprise the blood plasma that PC is higher.Can carry out this method, make the step of removing fluid repeat at least twice from interface one side.
This method can also may further comprise the steps: interface is radially inwardly moved, thereby make this interface itself near hole or opening, by this hole or opening blood plasma or blood platelet are removed.Remove under the situation of step carrying out at least twice, consider to make this interface turn back to its initial position this interface being moved near before this hole.
Another kind of method of the present invention comprises the method that is used to handle whole blood, and it can be used for reducing contributor or other time of being connected with blood separating mechanism of people or blood source under test.This method may further comprise the steps: the blood source is connected in separator; The blood introducing is installed formed centrifugal field by this; And permission forms interface between at least two kinds of compositions.This method is further comprising the steps of: from this interfacial side, remove first blood constituent from centrifugal field; From this interfacial opposite side, from this centrifugal field, remove second blood constituent; Store one of them kind of this first blood constituent and second blood constituent; Another kind with this first blood constituent and second blood constituent turns back to the blood source at least in part; And from the other blood of this blood source extraction.After having extracted other blood, be connected with this blood source disconnection, and repeat blood is introduced the step that this first blood constituent and second blood constituent are removed in this centrifugal field neutralization.As previously mentioned, for application subsequently, first blood constituent and second blood constituent of having been removed from centrifugal field can be stored.
Description of drawings
Fig. 1 is the perspective view that is ideally suited the fluid handling system that carries out blood processing, comprise blood processor (illustrating) and disposable liquid and blood flow utensil with the closure state that is used to transport and store, itself and this blood processor interacts, thereby isolate one or more blood constituents (illustrate with the form that is packaged in the pallet, be used for transportation and storage before using);
Fig. 2 is the perspective view of blood processor shown in Figure 1, and the open mode of work is shown;
Fig. 3 is the perspective view of blood processor shown in Figure 2, open admitting blood processing chamber and pump for wherein centrifugal, and the valve platform is opened with the pressure actuated box of admitting fluid;
Fig. 4 is the perspective view of blood processor shown in Figure 3, wherein comprises the pallet location of disposable liquid and blood flow utensil, is used for this mobile utensil is loaded in this blood processor;
Fig. 5 and Fig. 6 are respectively the right side and the left side perspective view of this blood processor after being loaded in the blood flow utensil on the blood processor shown in Figure 2;
Fig. 7 is the perspective view of blood processing chamber and the umbilical canal that joined, and this umbilical canal forms Fig. 5 and this liquid shown in Figure 6 and the part of blood flow utensil;
Fig. 8 is the perspective view of inside of first embodiment of the blood processing chamber of type shown in Figure 7, and this blood processing chamber can utilize Fig. 5 and blood processor shown in Figure 6 to carry out that red blood cell separates and collection process or other process;
Fig. 9 is the perspective view of inside of the centrifugal board of Fig. 5 and blood processor shown in Figure 6, and wherein this door of centrifugal is opened, to admit the blood processing chamber of type shown in Figure 7;
Figure 10 is the perspective view that the blood processing chamber of type shown in Figure 7 has loaded afterwards standby centrifugal inside shown in Figure 9;
Figure 11 is the schematic diagram of the blood processing chamber interior of type shown in Figure 7, and the separation of whole blood that illustrates under the normal condition becomes layer of red blood cells, plasma layer and middle buffy coat, and the position of these layers is shown;
Figure 12 is the schematic diagram of the blood processing chamber interior of type shown in Figure 7, and wherein buffy coat has moved very near low G wall, forms the state that the buffy coat composition is swept the overflow in the blood plasma of being gathered;
Figure 13 is the schematic diagram of the blood processing chamber interior of type shown in Figure 7, and wherein buffy coat has moved very near high G wall, forms the state of the underflow that the erythrocytic hematocrit that causes being gathered reduces;
Figure 14 is the top perspective view of inside of second embodiment of the blood processing chamber of type shown in Figure 7, and it can utilize the blood processor that is shown in Fig. 5 and Fig. 6 to carry out that blood plasma separates with blood platelet and gatherer process or other process;
Figure 15 is the bottom perspective view of blood processing chamber shown in Figure 14;
Figure 16 is the side perspective that the interior zone of blood processing chamber shown in Figure 14 amplifies, and the dividing plate with conical surface is shown, and this dividing plate is directed to red blood cell one path of opening with plasma separation from the Disengagement zone;
Figure 17 is the bottom perspective view of the amplification in zone shown in Figure 16, illustrate when red blood cell by dividing plate when the Disengagement zone guides, the path that they are walked.
Figure 18 is the bottom perspective view of the amplification in zone shown in Figure 16, illustrate when red blood cell and blood plasma by dividing plate when the Disengagement zone guides, the separation path that they are walked.
Figure 19 is the perspective view of inside of the 3rd embodiment of this blood processing chamber shown in Figure 7, the inside of this chamber can utilize Fig. 5 and blood processor shown in Figure 6 to carry out that fluid separates and gatherer process, and one of them of opposite end walls part and the isolated partial view of remainder of this chamber are shown;
Figure 20 is the top view of the inside of this chamber shown in Figure 19;
Figure 20 A is the part top view of amplification of the acquisition zone of this chamber shown in Figure 20;
Figure 21 is the bottom perspective view of this chamber shown in Figure 19;
Figure 22 is the perspective view of this chamber shown in Figure 19, and wherein the part of this chamber illustrates with cutaway view;
Figure 23 is the perspective view of inside of the 4th embodiment of the blood processing chamber of type shown in Figure 7, and wherein the end wall portion at top is removed, and this chamber can utilize Fig. 5 and blood processor shown in Figure 6 to carry out that fluid separates and gatherer process;
Figure 24 is the part top view of this chamber shown in Figure 23;
Figure 23 A is the perspective view that is similar to the 5th embodiment of this chamber shown in Figure 23, except the chamber shown in Figure 23 A does not have any outlet pathway of split tunnel in the upstream of dividing plate;
Figure 24 A is the perspective view that the fluid of this chamber shown in Figure 24 flows, and wherein this chamber is removed, so that be illustrated in the fluid path of this inside, chamber;
Figure 25 is the 6th embodiment of the blood processing chamber of type shown in Figure 7, and the inside of this chamber is configured to utilize Fig. 5 and blood processor shown in Figure 6 to carry out that blood platelet separates and gatherer process;
Figure 26 is the top view of this chamber interior shown in Figure 25;
Figure 27 is the part perspective view of this chamber shown in Figure 25, and wherein the part of this chamber illustrates with cutaway view;
Figure 28 is the perspective view of the 7th embodiment of the blood processing chamber of type shown in Figure 7, and the inside of this chamber is configured to utilize Fig. 5 and blood processor shown in Figure 6 to carry out that fluid separates and gatherer process;
Figure 29 is the part top view of this chamber shown in Figure 28;
Figure 30 is the part perspective view of this chamber shown in Figure 28, and the part of this chamber illustrates with cutaway view;
Figure 31 is the top view of the 8th embodiment of the blood processing chamber of type shown in Figure 7, and the inside of this chamber is configured to utilize Fig. 5 to carry out various fluids with blood processor shown in Figure 6 to separate and gatherer process;
Figure 32 is the partial left side perspective view of the amplification of this chamber shown in Figure 31;
Figure 33 is the partial top view of amplification of the circle segment of this chamber shown in Figure 31;
Figure 34 is the local top perspective view of the amplification of this chamber shown in Figure 31, and this chamber part illustrates with cutaway view;
Figure 35 is the top view of the 9th embodiment of the blood processing chamber of type shown in Figure 7, and it is configured to utilize Fig. 5 to carry out various fluids with blood processor shown in Figure 6 to separate and gatherer process;
Figure 36 is the fragmentary, perspective view of the amplification of this chamber shown in Figure 35, and the part of lateral wall part is removed;
Figure 37 is the partial top view of the amplification of this chamber shown in Figure 35;
Figure 38 is the top view of another amplification of the part of this chamber shown in Figure 37;
Figure 39 is the top view of the tenth embodiment of the blood processing chamber of type shown in Figure 7, and it is configured to utilize Fig. 5 and blood processor shown in Figure 6 to carry out that fluid separates and gatherer process;
Figure 40 is the top view of this chamber shown in Figure 39;
Figure 40 A is the top view of the amplification of this chamber shown in Figure 39;
Figure 41 is the perspective view that the fluid in this inside, chamber shown in Figure 39 flows, and this chamber is removed, so that the path that is positioned at this indoor fluid is shown;
Figure 42 A is the partial sectional view along 42A-42A line shown in Figure 40;
Figure 42 B is the partial sectional view along 42B-42B line shown in Figure 40;
Figure 43-the 45th according to one of fluid acquisition method described here, can carry out the schematic diagram of fluid circulation;
Figure 45 A is in red blood cell and the radially inside sample of gathering of blood plasma interface, the curve map of expression cycle rate (ml/min) and PC, and this sample is gathered after predetermined cycle period;
Figure 45 B is in red blood cell and the radially inside sample of gathering of blood plasma interface, the curve map of expression cycle rate (ml/min) and white blood cell count, and this sample is gathered after predetermined cycle period;
Figure 45 C illustrates and is illustrated in circulation time, at the curve map of the concentration of viewed blood platelet of different time and white blood corpuscle;
Figure 46 is the schematic diagram of inside of the blood processing chamber of type shown in Figure 7, and the separation of whole blood of the another kind method according to the present invention is shown, and this method may further comprise the steps: reduce centrifugal force, to expand at least one separating layer;
Figure 47 and Figure 48 are the schematic diagrames that can circulate according to the fluid that another kind of method described herein is carried out;
Figure 49 is according to one of method described herein, illustrate the fluid composition relevant with the blood source at least a portion collection and return the figure of circulation.
The specific embodiment
Fig. 1 illustrates the liquid processing system 10 that embodies feature of the present invention.This fluid handling system 10 can be used in handles various fluids.This system 10 especially is suitable for handling other suspension of whole blood and biological cellular material.Therefore, shown embodiment illustrates and is used for this purpose system 10.
I. systematic review
System 10 comprises two critical pieces.These two parts are: (i) blood processor 14---and be shown among Fig. 1 and be used to the closure state that transports and store, and in Fig. 2 and Fig. 3, be shown the open mode that is used for work; (ii) liquid and blood flow utensil 12, it and blood processor 14 interact, thereby separate and gather one or more blood constituents---this utensil in Fig. 1 and Fig. 4, be shown before using, be used for transporting and storing be packaged in pallet 48, and pull down and be installed on the blood processor 14 standby from this pallet 48 at Fig. 5 and Fig. 6.Though the each several part of system 10 will further describe, the details of this system is described in and is incorporated in these above-mentioned one or more patents or patent application for reference.
A. treating apparatus
Blood processor 14 is intended that the durable goods that can use for a long time.Shown in preferred embodiment in, this blood processor 14 is installed in portable shell and housing 36 the insides.This housing 36 provides compact table top, is suitable for being arranged on desktop or other the smaller surface and operation thereon, and this housing 36 also is intended to be easily transported to the collection position.
Housing 36 comprises base 38 and with gemel lid 40, and in order to transport, this lid 40 is closed (as shown in Figure 1), and in order to use, this lid 40 is opened (extremely shown in Figure 4 as Fig. 2).In use, this base 38 is intended to be used for being placed on the stayed surface of approximate horizontal.Housing 36 is for example by molded and can form desirable structure.Housing 36 is preferably made by in light weight, also durable plastics.
On this blood processor 14, controller 16 has been installed.Interaction between the parts of the parts of these controller 16 these blood processors 14 of control and the utensil 12 that should flow is with blood processing and the gatherer process that carries out being selected by the operator.In an illustrated embodiment, this controller 16 comprises Main Processor Unit (MPU), and this Main Processor Unit can comprise the Pentium microprocessor that for example Intel Company produced, but also can use the microprocessor of the routine of other type.MPU can be installed in lid 40 the insides of this housing 36.Has other parts power supply of the power supply 184 of power line for MPU and this blood processor 14.
Preferably, this controller 16 also comprises reciprocation user interface 42, and it allows the operator to observe and comprehensively about the information of the operation of this system 10.In an illustrated embodiment, this interface 42 is set on the interface screen that is installed on this lid 40, and this interface screen shows information and the graph image by the observed alphanumeric style of operator.
The details of controller 16 can find in No. 6,261,065, people's such as Nayak United States Patent (USP), and this patent is incorporated into that this is for reference.Other details of interface can find in No. 5,581,687, people's such as Lyle United States Patent (USP), and this patent is incorporated into also that this is for reference.
As shown in Figure 1, lid 40 can be used to support other input/output device, other external device (ED) is connected in other parts of controller 16 or this blood processor 14.For example, can be easily at other the local Ethernet interface 50 of installing on the outside of lid 40 or on the housing 36 of this blood processor 14, or be used for the input unit (being used for information scanning) of bar code reader etc. to controller 16, or diagnose interface 54, or in the blood processing process, be connected in the interface 56 (seeing for example Figure 43-45 and 47-48) that has on by the contributor with the pressure cuff 60 that strengthens blood flow rate, or system's conversion calibration interface 58, so that access.
B. utensil flows
The utensil that should flow is 12 that be intended to sterilize, the disposable of single use.Before beginning given blood processing and gatherer process, each parts and this blood processor 14 that the operator loads this mobile utensil 12 link (as shown in Figure 4 and Figure 5).Consider other input form of operator, controller 16 carries out this process according to predefined agreement.After finishing this process, the operator pulls down being connected of this mobile utensil 12 and this blood processor 14.That part of of mobile utensil 12 of the blood constituent that maintenance is gathered pulled down from this blood processor 14, and is preserved for storing, transfuses blood or further handles.The remainder that is somebody's turn to do the utensil 12 that flows is pulled down and is abandoned from blood processor 14.
The utensil that should flow comprises blood processing chamber 18, fluid-actuated pump and valve cap 28 and with container handling 64 be connected in the array that the flow line of this blood processing chamber 18 and this valve cap 28 links.To describe several embodiment of this blood processing chamber 18 below in detail.
1. blood processing chamber
(see figure 5) in this embodiment, this mobile utensil 12 comprise and are designed to the blood processing chamber 18 that links to each other with centrifuge.This treating apparatus 14 comprises centrifugal board 20 (seeing Fig. 2 and Fig. 3), and it admits this process chamber 18 (see figure 5)s, so that use.
As shown in Figures 2 and 3, this centrifugal board 20 comprises the compartment 24 that is formed in the base 38.This centrifugal board 20 comprises door 22.Door 22 is opened (as Fig. 3 and shown in Figure 5), so that process chamber 18 can be loaded in this compartment 24.At run duration, this door 22 (as Fig. 2 and shown in Figure 6) is closed, thereby this process chamber 18 is enclosed in this compartment 24.
Centrifugal board 20 is closed this process chamber 18.During rotation, this process chamber 18 preferably will receive the separation of whole blood of coming from the contributor and become some parts with the centrifugation separation of the fluid, mainly be red blood cell, blood plasma and the intermediate layer that is called buffy coat, and it is filled by blood platelet and white blood corpuscle.As will be described below, the structure of process chamber 18 can change according to the blood separate object of being wanted.Several embodiment of this process chamber 18 will be described below.
2. fluid pressure actuated box
In an illustrated embodiment, this mobile utensil 12 also comprises fluid pressure actuated box 28 (see figure 5)s.This pressure actuated box 28 provides concentrated, programmable, integrated platform, is used for the function at needed whole pumpings of given processing procedure and adjusting flow.In an illustrated embodiment, fluid pressure comprises positive and negative Pneumatic pressure, but can use the fluid pressure of other type.
As shown in Figure 5, valve cap 28 is mounted for using in pneumatically actuated pump and valve platform 30, and this pump and valve platform 30 are arranged in the lid 40 of this housing 36.This pump and valve platform 30 comprise hinged door 32, in order between the opening and closing position, to move, wherein in open position, expose the pump and valve platform 30 (see figure 3)s that are used for this valve cap 28 of loading and unloading, locate in the closed position, valve cap 28 is enclosed in (see figure 6) in this pump and the valve platform 30, so that use.This pump and valve platform 30 comprise manifold component 34 (see figure 4)s that are positioned valve face packing ring back when this valve cap 28 is installed on pump and the valve platform 30.This pneumatic pressure guide liquid flows through this valve cap 28.
3. blood processing container and pipeline
As illustrated in Figures 5 and 6, this mobile utensil 12 also comprises pipeline and the vessel array that is communicated with this valve cap 28 and these process chamber 18 fluids.The layout of pipeline and container can change according to the object of handling.Representational blood processing process and the relevant mobile utensil that this process is provided will be described subsequently.
Umbilical canal (aumbilicus) 100 has formed the part of this utensil 16 that flows.During installation, these umbilical canal 100 connections have the rotation process chamber 18 of this valve cap 28, and do not need rotating seal.This umbilical canal 100 can be made by the plastic material of rotation stress proofing, for example Hytrel copolymerization fat elastomer (E.I.Du Pont Company).
With reference to figure 7, pipe 102,104 and 106 proximal ends from this umbilical canal 100 extend out.This pipe 102 is delivered into whole blood in this process chamber 18, so that separate.Pipe 104 and 106 is respectively applied for from this process chamber 18 and carries isolated red blood cell and blood plasma.According to the object of handling, blood plasma is can platelet content higher or lower.
As shown in Figure 7, have anchor clamps 108 with pipe 102,104 and 106 with outside compactness, coherent, that array way side by side accumulates in centrifugal board 20, near this umbilical canal 100.These anchor clamps 108 make pipe 102,104 and 106 can be positioned to 46 one-tenth one group of optical sensor platform and become assembling and dismantling to unload (seeing Fig. 9 and 10), and this optical sensor platform 46 is near centrifugal board 20 location in these process chamber 18 outsides.
This optical sensor platform 46 is monitored by the existence of target blood constituent (for example red blood cell and blood platelet) in pipe 104 and 106 blood of being carried optically or is not existed.This induction platform 46 provides this blood constituent of reflection to exist or non-existent output.This output is transferred to controller 16.This controller 16 partly should be exported according to the optical sensor result treatment and produce signal, to control this result.This controller has been described in the details of controlling result according to the optical sensor operation and has been engaged in these above-mentioned one or more patents or patent application for reference.
(see Fig. 5 and 6) as shown in the figure, this mobile utensil 16 comprises intravenous needle 128, by this intravenous needle 128, has one can be connected in this system 10, is used for blood processing.In Fig. 5 and Fig. 6, this mobile utensil 16 also comprises blood sampling assembly 110.When given blood processing process began, this blood sampling assembly 110 can be gathered one or more samples of contributor's blood by intravenous needle 128.Be provided with conventional manual folder 114 (for example, the Roberts folder), flow to sampling component 110 with control blood.
As shown in Figure 5 and Figure 6, this mobile utensil 16 can comprise straight line (in-line) formula injection point 112.This injection point 112 make the technical staff can with saliferous or other physiological fluid or medicine introduce among the contributor.If desired, then utilize intravenous needle 128, and do not need extra syringe needle.Other orthoscopic is manually pressed from both sides the upstream end that 116 hope are included in blood sampling assembly 110 and injection point 112.The utensil 16 that should flow can comprise such as T form point, Y form point, suitable joint V-arrangement point or that other connector is arranged.
This blood processor also comprises the container support of one or more weighing units 62 and other form.Certainly, the setting of these parts on this blood processor 14 can change according to handling object.By way of example rather than ways to restrain, Fig. 5 and Fig. 6 illustrate and are respectively applied for the erythrocytic collection container 158,160,162 and 172 that reduces at goods (in-process) (or whole blood), blood plasma, red blood cell and white blood cell.In Fig. 5 and Fig. 6, other holder and container 150,164 and 168 can comprise and be used for various other fluids of using in processing procedure, such as but not limited to anti-coagulants, salt solution and preservation or storage solutions.During processing, admitted into container and/or dispense from container along with blood or liquid, weighing unit 62 provides the output of reflection weight time to time change.This output is transferred to controller 16.Controller 16 is handled the weight change that increases, thereby draws the fluid treatment volume.Controller produces signal partly according to the processing volume that is drawn, with the control result.
C. centrifugal board
Centrifugal board 20 (see figure 9)s comprise centrifuge assembly 68.This centrifuge assembly 68 is configured to admit and supports molded process chamber 18 and umbilical canal 100, so that use.
As shown in Figure 9, this centrifuge assembly 68 comprise have diapire 72, the framework or the yoke 70 of roof 74 and sidewall 76.This yoke 70 is gone up rotation at the supporting member 78 (Fig. 9) that is connected in diapire 72.There is motor 80 to be connected in the diapire 72 of this yoke 70, uses so that this yoke 70 rotates around axis 82.In the embodiment shown, axis 82 is the (see figure 3) of level substantially, but also can be with other angle orientation.According to the order that controller 16 is sent, this motor 80 can be to rotate this yoke 70 clockwise or counterclockwise.
Supporting member 86 rotations around himself in yoke 70 of carriage or rotating disk 84 are arranged, and this supporting member 86 is connected in the roof 74 of this yoke 70.This rotating disk 84 is around the axis rotation of aliging with the rotation 82 of this yoke 70 basically.
As shown in Figure 7, the top of process chamber 18 comprises the ring cover 220 that cover 202 is fixed thereon.As shown in figure 10, this rotating disk 84 comprises the latch assembly 88 that clamps this lid 220 with removably, is used for this process chamber is fixed in this rotating disk 84, so that rotation.The details of latch assembly 88 can find in above-mentioned one or more patents or patent application, and these patents or patent application have been incorporated into that this is for reference.
As shown in figure 10, near umbilical canal 100 ends, there is sheath 144 to be installed in the recessed bag 90 of the shaping in advance on this centrifugal board.These recessed bag 90 near-ends with this umbilical canal 100 remain on the non-rotary resting position, align with the rotation that aligns mutually 82 of yoke 70 and rotating disk 84 in this non-rotary position.Pipe 102,104 and 106 and the induction platform 46 that links be placed with one group and dismantle in groups with this induction platform 46, this induction platform also is positioned at this recessed bag 90, as shown in figure 10.
Umbilical canal drives or support component 92 and 94 and passage 96 and 98 (seeing Fig. 9 and the 10) part of admitting this umbilical canal 100.Yoke 70 makes this umbilical canal 100 keep not reversing with an omega rotating speed and rotating disk 84 with the relative rotation of two omega rotating speeds, needing to have avoided seal.Other details of this set is disclosed in people's such as Brown No. 4,120,449, the United States Patent (USP), and this patent is incorporated into this common reference, also is disclosed in to be incorporated in these above-mentioned one or more patents or patent application for reference.
D. control by the interface of optical sensor
In above-mentioned any blood processing process, be presented in this process chamber centrifugal force with blood be separated into filling red blood cell district and blood plasma district (as Figure 11 schematically shown in).Centrifugal force makes that district of filling red blood cell assemble along the radial outside or the high G wall of this process chamber, and the blood plasma district is transferred to the radially inner side or the low G wall of this process chamber.
The mesozone forms interface between this red blood cell district and blood plasma district.Cell blood material as blood platelet and leukocytic intermediate density forms this interface according to density, wherein blood platelet than white blood cell more near plasma layer.Because compare with the redness in red blood cell district with the straw look in blood plasma district, interfacial cloud (cloud) color, thereby this interface also is called " buffy coat ".
Can wish to monitor the position of this buffy coat, thereby according to this process, perhaps the material with this buffy coat remains on the blood plasma outside, perhaps remains on the red blood cell outside, perhaps gathers the cellular material of this buffy coat.For this reason, this system comprises the optical sensor platform 46 that two optical sensor assemblies are housed, and schematically illustrates among Figure 11,12 and 13.
First inductive component 146 in this induction platform 46 is monitored the passage by the blood constituent of sampled plasma pipe 106 optically.Second sensing component 148 in this induction platform 46 is monitored the passage by the blood constituent of red blood cell collection tube 104 optically.
Pipe 104 and 106 is by making transparent plastics (for example polyvinyl chloride) material of luminous energy that is used for responding to, and this luminous energy is at least in pipe 104 and 106 is placed the zone that links with induction platform 46.Anchor clamps 108 are held in pipe 104 and 106 and corresponding inductive component 148 and 146 visual alignment.These anchor clamps 108 also keep whole blood is flowed to the pipe 102 of centrifugal board 20.Anchor clamps 108 are used for assembling and keeping being connected in compactness and easy to handle colligation mode all pipes 102,104 and 106 of umbilical canal 100.
This first inductive component 146 can detect the cellular material kind or the composition of the optical laying in the sampled plasma pipe 106.The cell component that optical laying is used to detect changes according to process.
When first inductive component 147 detects when having blood platelet in blood plasma, it indicates the low G wall of this this process chamber of interface close enough, thereby makes all the components or some of them composition be swept into sampled plasma pipeline (seeing Figure 12).This state also is called " overflow (over spill) ".
Second inductive component 148 can detect the hematocrit (hematocrit) of the red blood cell in the red blood cell collection tube 104.During handling, the reducing of the hematocrit of red blood cell is lower than the minimum of a value of setting and represents the high G wall of this this process chamber of interface close enough, thus make compositions all in this interface or some of them and perhaps the blood plasma of this interface opposite side can enter red blood cell collection tube 104 (seeing Figure 13).This state is called " underflow (underspill) "
II. the embodiment of blood processing chamber
112 below will describe several embodiment of blood processing chamber.These blood processing chambers can use with the mobile utensil 12 with this blood processor 14 and controller 16 combinations, to carry out various gatherer processes.
A. first embodiment of blood processing chamber
Fig. 8 illustrates the embodiment of centrifugal treating chamber 198, and this process chamber can be used in combination with system 10 shown in Figure 1, to carry out two unit red blood cell gatherer processes and other process.This process chamber 198 is manufactured into two moulded parts of separating, i.e. pedestal 201 and lid 202.Axle sleeve 204 is radially centered on by inboard that limits peripheral blood split tunnel 210 and exterior annular wall 206 and 208.Molded annular wall 214 (see figure 7)s are sealed the bottom of this split tunnel 210.Lid 202 for example is fixed in the top 200 of this process chamber by means of the sonic welded horn shape loudspeaker (horn) of cylindricality.
Interior annular wall 206 is open between a pair of sclerine, and this forms open inner area 222 to sclerine in axle sleeve 204.Blood and fluid are introduced into this split tunnel 210 and draw this split tunnel 210 from umbilical canal 100 by this inner area 222.The molded inwall 224 that is formed in this inner area 222 extends across this split tunnel 210 fully, is connected in exterior annular wall 108.Wall 224 forms terminal point in split tunnel 210, between separation period, this terminal point interrupts flowing along the circumference of this split tunnel 210.
Molded inwall in addition is divided into three passages 226,228 and 230 with this inner area 222.These passages 226,228 and 230 extend out from axle sleeve 204, and are communicated with split tunnel 210 at the opposite side place of terminal point wall 224.Blood and other fluid are by these passages 226,228 and 230 and be directed passing in and out this split tunnel 210 from axle sleeve 204.
When process chamber shown in Figure 8 198 rotations (the arrow R among Fig. 8), umbilical canal 100 is delivered into whole blood in this split tunnel 210 by passage 226.Whole blood flows along the direction identical with rotation (being counterclockwise in Fig. 8) in split tunnel 210.Perhaps, though the efficient of separating for blood, whole blood flows along the direction identical with rotation and it is believed that and wishes, and this process chamber 198 can promptly, turn clockwise along rotating with the direction of the circumference flowing opposite of this whole blood.
As the result with the centrifugal force of mode shown in Figure 11, separation of whole blood.Red blood cell is driven by the high G wall 208 towards radial outside, and lighter blood plasma constituent moves towards the low G wall 206 of radially inner side.
As shown in Figure 8, a baffle plate 244 enters in this split tunnel 210 towards high G wall is outstanding.This baffle plate or dividing plate 244 prevent that blood plasma from passing through, and allow red blood cell by entering passage 246 recessed in high G wall 208.This passage 246 guides red blood cell to enter umbilical canal 100 by radial passage 230.The blood plasma constituent is transported to this umbilical canal 100 by radial passage 228 from split tunnel 210.
Because red blood cell exit passageway 246 extends to outside this high G wall, further be spaced apart than this high G wall from this rotation, thereby this red blood cell exit passageway 246 can locate the interface between red blood cell and the buffy coat very near this high G wall 208 during the blood processing, and do not make buffy coat overflow into this red blood cell acquisition channel 230 (formation underflow condition).Thereby this recessed exit passageway 246 allows the output maximum (in the red blood cell gatherer process) of red blood cell or gathers essentially no hematoblastic blood plasma (in the process of gathering blood plasma).
B. second embodiment of blood processing chamber
Figure 14 illustrates the embodiment of centrifugal treating chamber 200, and it can use in conjunction with system shown in Figure 1 10, so that carry out the sampled plasma process, providing does not have or do not have basically blood platelet, red blood cell and leukocytic blood plasma.The blood plasma that process chamber 200 shown in Figure 14 can also be used to make up/red blood cell gatherer process, the red blood cell that its branch comes and gathers blood plasma and concentrate, and such as gathering hematoblastic other process, the blood platelet that this process collection concentrates and the mixture of blood plasma.
As the front about the embodiment of process chamber shown in Figure 8 described (same parts are given same Reference numeral), though this process chamber embodiment and other the process chamber embodiment that is discussed in summary of the invention can adopt other structure and not break away from broad aspect of the present invention, this process chamber 200 wishes to be manufactured into molded base component 201 and cover 202 separately.The madial wall part and the lateral wall part wall 206 and 208 of circumference blood split tunnel 210 radially center on molded axle sleeve 204 by limiting roughly.Molded wall 214 (seeing Figure 15) forms the end wall portion of this split tunnel 210.Cover 202 forms another end wall portion of this split tunnel 210 and can also comprise insert 242.Though two relative end wall portion are shown (that is, perpendicular to rotation) of making general flat, and sidewall sections 206 and 208 shows and makes substantial cylindrical, is to be understood that this border can be shapes such as taper, circle, V-arrangement.During assembling, cover 202 is by for example being fixed on the top of this process chamber 200 with cylindrical sonic welded horn shape loudspeaker.
In this process chamber 200 shown in Figure 14, madial wall part 206 is open between a pair of sclerine.This open sclerine forms open inner area 222 in axle sleeve 204, it is communicated with split tunnel 210.Blood and fluid are introduced into and draw this split tunnel 210 through this inner area 222 from umbilical canal 100.
In the embodiment shown in fig. 14, molded inwall 224 is formed in the inner area 222 that extends across this split tunnel 210 fully, is connected in lateral wall part 208.This inwall 224 forms terminal point in split tunnel 210, between separation period, this terminal point interrupts flowing around this split tunnel 210.
Molded inwall in addition is divided into three passages 226,228 and 230 with this inner area 222.These passages 226,228 and 230 extend out from axle sleeve 204, and are communicated with split tunnel 210 at the opposite side of terminal point wall 224.Blood and other fluid are directed passing in and out this split tunnel 210 by these passages 226,228 and 230 from axle sleeve 204.
When process chamber 200 rotations (the arrow R among Figure 14), umbilical canal 100 (not shown) are transported to passage 226 with whole blood, and this passage 226 leads to this split tunnel 210.Whole blood flows along the direction identical with rotation (being counterclockwise in Figure 14) in split tunnel 210.Perhaps, though for optimization blood separates, whole blood flows along the direction identical with rotation and it is believed that and wishes, and process chamber 200 can promptly, turn clockwise along rotating with the direction of the circumference flowing opposite of this whole blood.
As the result with the centrifugal force of mode shown in Figure 11, whole blood separates in this process chamber 200.Red blood cell is driven towards lateral wall part or high G wall 208, and lighter blood plasma constituent moves towards low G wall 206.Buffy coat is present between this inside and outside sidewall sections 206 and 208.
Almost 360 degree are circumferentially spaced is sampled plasma passage 228 and red blood cell acquisition channel 230 for contiguous terminal point wall 224 and whole blood access road 226.Along the upstream flow direction of these acquisition channels 228 and 230, a dividing plate 232 is from high G wall 208 outstanding this split tunnels 210 that enter.This dividing plate 232 forms contraction along madial wall part or low G wall 206 in split tunnel 210.On the circumference flow direction of blood, sampled plasma passage 228 is led in this contraction.
As Figure 16 and shown in Figure 17, on the direction of this terminal point wall 224, the leading edge 234 of this dividing plate 232 is tapered towards the annular boundary (in an illustrated embodiment, being annular wall 214) of this split tunnel 210.The edge 234 that this dividing plate 232 attenuates leads to towards the opening 236 of the annular boundary of this split tunnel 210.This opening 236 is towards the annular boundary of very close this high G wall 208, and is still axially spaced with it.Opening 236 is connected with red blood cell acquisition channel 230.
In opening 236, there is shoulder 238 radially to extend an axial distance from low G wall, this shoulder 238 shrinks the radial dimension of this opening 236 along radial outside or high G wall 208.Because this shoulder 238 has only the red blood cell of contiguous this high G wall 208 and the composition of other higher density to be communicated with this opening 236.This shoulder 238 keeps and the blood plasma that keeps clear of this high G wall 208 is not communicated with this opening 236.Owing to,, do not have the place to flow except towards sampled plasma passage 228 along the radially affined opening 236 of high G wall 208.Thereby the blood plasma that leaves split tunnel 210 does not have or does not have basically the material of higher density, and the material of higher density leaves this split tunnel 210 by this affined high G opening 236.
This shoulder 238 connects axial surface 240, and this axial surface 240 aligns with low G wall 206 substantially.This axial surface 240 extends axially red blood cell acquisition channel 230 along rotation.Because dividing plate 232, shoulder 238 and other inwall, red blood cell acquisition channel 230 is kept apart (as shown in figure 18) with sampled plasma passage 228.Also as Figure 18 the most clearly shown in, the blood plasma that exists along low G wall 206 is directed to umbilical canal 100 circumferentially by dividing plate 232 and shoulder 238.The fluid of higher density comprises red blood cell, and according to the process that is adopted, can also comprise buffy coat composition (blood platelet and white blood cell).The fluid of this higher density exists near high G wall 208, and is axially guided towards annular boundary and affined high G opening 236 by the edge 234 along tapered dividing plate 232.From this high G opening 236, red blood cell and the buffy coat that comprises fluid of higher density constitute thing and are crossed this radially shoulder 238 towards these low G wall 206 guiding, and are entered in this red blood cell acquisition channel 230 and enter this umbilical canal 100 by guiding axially.
C. the 3rd embodiment of blood processing chamber
In Figure 19-22, process chamber is represented with 300.This process chamber 300 can use in conjunction with system shown in Figure 1 10, so that carry out the various gatherer processes of various biofluids, comprises but is not specifically designed to blood.The gatherer process that this process chamber 300 can be used to carry out blood platelet or be rich in hematoblastic blood plasma (PRP)---gather the concentrated blood platelet and the mixture of blood plasma; The red blood cell and the sampled plasma process of combination---divide the red blood cell that comes and gather blood plasma and concentrate; The red blood cell and the hematoblastic gatherer process of combination---dividing comes gathers the red blood cell that concentrates and the blood platelet of concentrating; And other process.
This process chamber 300 comprises molded pedestal 301 separately, and it has the axle sleeve 304 that is provided with along the axis A of this process chamber.The pedestal 301 of this process chamber 300 comprises interior (low G) sidewall 306 and outer (high G) sidewall 308 that is radially spaced respectively.In this specification, these sidewalls are known as radially inner side (low G) wall 306 and radial outside (high G) wall 308 all the time.Should inside and outside sidewall sections 306 with 308 and relative end wall portion 302 and 304 limited substantially around (it is not limited to circular) blood split tunnel 310.First end wall portion 314 forms the axial boundary or the bottom of these split tunnels 310, second end wall portion or cover another axial boundary or the top that 302 (partly illustrating among Figure 19) form this split tunnel 310 substantially.
Though inside and outside wall part 306 and 308 is shown circumference basically, that is, have roughly the vertical substantially wall of radius uniformly with respect to shared axis A, other orientation, shape, axle and radius also are fine.And, though top and bottom end wall partly are shown general planar, these end wall portion also can have such as curved surface, circular arc etc. other shape.The shape of this split tunnel and directed can also depend on this split tunnel by flexible, semi-rigid still be that rigid structure is made.The structure that should be appreciated that the end wall portion of picture " top " and " bottom " is not limited to these structures.These terms are arbitrarily, and only are used for making an end wall to differentiate mutually with another end wall in relation shown in the drawings, so that help to understand these structures.
Shown in Figure 20 and 20A, the upstream extremity of this split tunnel 310 comprises a pair of relative inner radial wall 322 and 324.This inner radial wall 324 connects lateral wall 308, and roughly this split tunnel 310 is separated at its upstream end and between the downstream.This interior wall 322 and 324 extends radially outwardly from axle sleeve 304, thus be formed for fluid, preferably be used for the access road 326 of whole blood, to enter this process chamber 300.This access road 326 forms at the top of this process chamber 300 substantially, or forms near this top, and as shown in figure 22, and preferably part is formed by the surface of this top end wall part 302.This inlet 326 comprises opening 325, and this opening 325 preferably is set at radial position place of contiguous this lateral wall part or high G sidewall sections 308, and this opening 325 is limited by the surface institute of inlet 326.The step of inner radial wall 322 or edge 323 radially are arranged on the centre of this madial wall part and lateral wall part 306 and 308, and preferably form the surface of this opening 325, and fluid is introduced in this split tunnel 310 by this opening.
In the downstream of this split tunnel 310, first, second and the 3rd outlet flow path 328,330 and 332 can limit and be used for a kind of and multiple fluid outlet pathway from split tunnel 310.Downstream end at this split tunnel 310 also is positioned with baffle plate or dividing plate 336, and it will be described in greater detail below.
In Figure 20 A, the first outlet flow path 328 limits between this dividing plate 336 and the inner radial wall 335 that extends radially outwardly from this axle sleeve 304.From the junction 352, dividing plate 336 is radially inside, and this first flow path limits between two inner radial wall 334 and 335.The first outlet flow path 328 comprises opening 327, and fluid enters from this split tunnel 310 by this opening 237.This opening 327 is preferably located in the upstream of this dividing plate 336, is in the radial position near the radial position of this madial wall part 306.
When this split tunnel 310 under the normal condition---promptly neither in underflow also not under overflow state---during operation, fluid in first flow path 328 preferably in the junction 352 radially inwardly (with the outside of this process chamber 300) flow, perhaps radially outward flow to the second outlet flow path 330 alternatively in this junction 352." normal condition " is meant that the composition of blood in this split tunnel 310 is separated into blood plasma, buffy coat and red blood cell, and preferably is arranged on relevant radial position place as shown in figure 11.Normal condition can also comprise that the blood constituent in this split tunnel 310 is separated into and be rich in hematoblastic blood plasma and red blood cell, and the interface between blood plasma and the red blood cell is set at the radially middle of this interior (low G) and outer (high G) wall, is similar to the interfacial radial position of Figure 11.
In Figure 20 A, the second outlet flow path 330 limits in the downstream of the first outlet flow path 328 usually, and between this dividing plate 336 and inner radial wall 334.This second outlet flow path 330 can be communicated with the downstream that the first outlet flow path 328 and the 3rd exports this dividing plate 336 between the flow path 332 by fluid.This second outlet flow path 330 comprises first opening 329, the preferably contiguous junction 352 of this opening 329, thus be communicated with the first outlet flow path, 328 fluids, but other position also is fine.Second opening 331 of the second outlet flow path 330 is preferably at the radial outside of first opening 329.
Under normal condition, the direction of the fluid mobile (for example blood plasma flows) in this second outlet flow path 330 makes fluid flow to opening 331 in the inner radial of this junction 352 substantially.The distance of the radial path that blood plasma flow through will depend on the interfacial radial position between blood plasma and the red blood cell in this second outlet flow path 330.Preferably, blood plasma flows to this second outlet flow path 330 from first flow path 328, to be full of this interfacial radially inner second outlet flow path 330, still not in this interfacial flowing radially outward.Under normal condition, will mainly flow out this process chamber 300 from first blood plasma that exports flow path 328, and in some blood plasma inflow second outlet flow paths 330, thereby fill the radially inner zone of this interface.
Though the preferred flow form of first flow path 328 and the second outlet flow path 330 more than has been discussed, can also be mobile at the first outlet flow path and the fluid in the second outlet flow path with different liquid forms.This liquid form can depend on the interfacial position that is associated with a kind of and multiple fluid composition and gather residing ratio of one or more fluid compositions and other factors from this split tunnel 310.By way of example rather than the mode of restriction, if the interface between blood plasma and the red blood cell radially moves inward the state of facilitating overflow, then can flow radially outward opening 329 by being positioned at junction 352 at the fluid of second outlet in the flow path 330.
In Figure 20 A, the 3rd outlet flow path 332 limits between this inner radial wall 334 and 324, and comprises opening 333.This opening 333 is preferably located in downstream and the first outlet flow path and the second outlet flow path 328 and 330 downstreams of dividing plate 336.Fluid can flow to this opening 333, enters in the 3rd outlet flow path 332, so that remove from this split tunnel 310.
Shown in Figure 20,20A and 22, this dividing plate 336 comprises upstream side 338 and downstream 340, and each side all is approximately perpendicular to lateral wall part 308.This dividing plate 336 roughly radially extends across this split tunnel 310 between the radial position corresponding to this madial wall part and lateral wall part 306 and 308.In Figure 20 A, this dividing plate preferably is arranged in this radially inner side of (low G) sidewall sections, and 352 is tapered to the junction along angled wall 342.This dividing plate 340 near or also comprise taper and curved in abutting connection with this lateral wall part 308.Though this dividing plate illustrate near or have the shape of taper in abutting connection with this madial wall part 306 and lateral wall part 308, this shape by way of example rather than the mode that limits illustrate, and to know that other shape also is possible.
As shown in figure 22, the upstream side 338 of this dividing plate 336 axially extends along sizable part of the axial length of this split tunnel 310 from the end wall portion 302 at the top that is positioned at this split tunnel 310.At these upstream side 338 places, the axial location of this dividing plate 336 ends at preferably and these end wall portion 314 spaced positions places.In such axial positions, first flow path 344 can be communicated with between the upstream side 338 of this dividing plate 336 and downstream 340.This first flow path 344 preferably is positioned the middle axial positions between relative end wall 302 and 314.In Figure 22, this first flow path 344 is illustrated near end wall portion 314, more particularly, is illustrated and is positioned at the axial positions that roughly is positioned in the end half of this process chamber 300 one or third part.In Figure 22, must pass sizable axial distance of this split tunnel 310 by 326 fluids that enter and flow to this first flow path 344 that enter the mouth.Axial location also is possible in the middle of other of this first flow path 344, for example along the centre position of this dividing plate 336.This first flow path 344 can also be located adjacent to or be close to the axial location of the bottom end wall part 314 of this split tunnel 310.
In Figure 20 and 20A, first flow path 344 is limited by one of this lateral wall part 308 and a plurality of first and second radially outer parts 309 and 311 along its outer radial face.This first 309 radially outward is tapered from the radial position than the part of upstream of this lateral wall part 308.This first 309 is positioned at the upstream of this dividing plate 336 substantially, and links to each other with second portion 311 at the downstream part of this dividing plate 336.Be positioned at comparing of this split tunnel 310 than the position of upstream, the i.e. radial position of this lateral wall part 308 of the upstream position of this part 309, this second portion 311 also is in radially outward.This second portion 311 preferably is arranged on the position same with first 311.The relative interior radially surface of first flow path 344 preferably is arranged on radial position place than upstream portion near this lateral wall part 308.
In Figure 20 and 20A, the acquisition zone that limits at 346 places is set at the downstream (being shown in broken lines) of dividing plate 336 usually.The top surface of this acquisition zone 346 is limited by the end wall portion 302 at the top that is positioned at this split tunnel 310.This acquisition zone 346 also comprises the intermediate ends wall part 348 (Figure 22) of at least a portion of the basal surface that defines it.The top of this intermediate ends wall part 348 and split tunnel 310 and the end wall portion 302 and 314 of bottom are axially spaced apart.Though this intermediate ends wall part 348 is shown the end wall portion 314 of parallel substantially this split tunnel 310, other orientation also is possible.
In Figure 20 A, this acquisition zone 346 is also partly limited by the downstream 340 of dividing plate 336 and the inwall 324 of split tunnel 310, and, in Figure 20 A, this acquisition zone 346 is set between the radial position corresponding to this madial wall part and lateral wall part 306 and 308 usually, and preferably limits between the radial position of the part 311 of madial wall part 306 and lateral wall part 308.
As shown in figure 22, this acquisition zone 346 comprises the axial lead opening 347 that is formed on the middle end wall 348.Axially upwards flow in the downstream 340 of fluid from first flow path 344 along this dividing plate 366, thereby enter the bottom of this acquisition zone 346 by this opening 347.As previously described, opening 331 and 333 (as Figure 20 A clearly shown in) can also fluid be communicated with the one or more fluid compositions that flow into or flow out this acquisition zone 346.In Figure 20 A, this acquisition zone 346 comprises the radially outer edge 350 of inner radial wall 334, and the edge 350 of this inner radial wall 334 is positioned between the opening 331 and 333 that leads to this second outlet flow path and the 3rd outlet flow path 330 and 332.This edge 350 is set at the intermediate radial position place between the part radially outward 311 that is positioned at this madial wall part 306 and outer (high G) sidewall sections 308.The radial position at this edge 350 is preferably located than the radial position near this part 311.This edge 350 is preferably set to make under normal operation, can leave the 3rd outlet flow path 332 such as the fluid of the higher density of red blood cell, and more low-density fluid is not then from wherein leaving.
During use, enter inlet 326 such as the fluid of whole blood and flow in this split tunnel 310.When fluid at first entered this split tunnel 310, this fluid was usually located at the top of this split tunnel 310.Begin to be limited in by base plate 354 (seeing Figure 22) at the axial distance that the fluid at opening 325 places of access road 326 flows on the following axial length of this access road 326 of porch.The axial location of this base plate 354 can be set at the axial positions near the axial location of the intermediate ends wall part 348 of this acquisition zone 346, but other position also is possible.After fluid entered split tunnel 310, this split tunnel 310 preferably no longer was constrained on it down on axial length, but it is still retrained thereon on the axial length by relative end wall portion 302.
In split tunnel 310, though other liquid form also is possible, when fluid during in downstream flow, it can be basically flows with spiral form (among Figure 22 shown in the dotted line), so that this fluid increases on its axial length usually.At the upstream end of dividing plate 336, the axial length of fluid is preferably set to from the top end wall part 302 at this split tunnel 310 tops at least near the axial location of this first flow path 344 or lower axial location.By utilizing volume as much as possible in this split tunnel, it is believed that the separation that can obtain more effective fluid composition.
When fluid during in downstream flow, centrifugal force makes the composition of blood radially separate in split tunnel according to density.The more detailed details of this separation is disclosed in Brown's " The physicsof Continuous Flow Centrifugal Sell Separation " literary composition, sees Artificial Organ, 13 (1): 4-20 (1998).
Figure 11 is illustrated in during the normal condition of split tunnel 310, in the relative radial position of the fluid composition of the upstream of this dividing plate 336.Blood plasma mainly is arranged near interior or low G sidewall sections 306 places, and red blood cell is arranged near outer or high G sidewall sections 308 places by main.Be called the blood platelet of " buffy coat " and white blood cell and mainly be arranged in interface between blood plasma and the red blood cell, and be positioned at the intermediate radial position place.For the platelets gathering process, as what be discussed in more detail below, preferably also carry out other treatment step, so that at least a portion platelet suspension is in blood plasma, so that be rich in hematoblastic blood plasma in the interfacial side formation of being rich between hematoblastic blood plasma and the red blood cell.
At the upstream end of this dividing plate 336,, can gather at least a fluid composition by this first outlet flow path 328.This composition can comprise the blood plasma PPP of anaemia platelet or be rich in hematoblastic blood plasma PRP.This composition also can flow in the second outlet flow path 330 in junction 352.Other fluid composition, be preferably red blood cell and can flow in first flow path 344, so that remove by the 3rd outlet flow path 332.If blood platelet mainly is arranged in buffy coat, then sizable part of this buffy coat is separated at upstream side 338 places of dividing plate 336 at least.In this respect, certain during handling a bit on, for example, by from this split tunnel 310 when removing, dividing plate 336 allows blood platelets to assemble at these dividing plate 336 upstream ends at the blood plasma PPP of anaemia platelet.Such process will be discussed further below.Therefore, at the downstream part of dividing plate 336, interfacial that part of between blood plasma and the red blood cell, with the upstream end at dividing plate 336, the interface between these compositions is compared, and preferably comprises considerably less blood platelet, does not perhaps have blood platelet in fact.
At the downstream part of dividing plate 336, between red blood cell and blood plasma, can form interface, this blood plasma can be or be rich in blood plasma hematoblastic or the anaemia platelet.Under normal operation, the interface between blood plasma and the red blood cell is positioned at the intermediate radial position place, between the promptly inside and outside sidewall sections.This interface is preferably located in the radially inside of first flow path 334, thereby mainly is that red blood cell flows through this first flow path 334 under normal operation.More particularly, the interface between red blood cell and the blood plasma is set at 350 radial position place of keeping to the side.Such radial position can export the flow path 332 red blood cell but make quite few blood plasma or do not have blood plasma to flow into the 3rd from this interfacial opposite side from collected the 3rd outlet flow path 332 of this interfacial side.Blood plasma and red blood cell mainly flow through the first outlet flow path and the 3rd outlet flow path respectively.Second flow path 330 preferably radially inwardly comprises blood plasma or is rich in hematoblastic blood plasma interfacial, and at this interfacial red blood cell that radially outward comprises.According to this interfacial radial position, some that can occur blood plasma and red blood cell in second outlet in the flow path 330 flows, but this flowing preferably do not change interfacial this position.
Other liquid form is possible, and can depend on this interfacial other radial position.For example, during the state of overflow,---promptly when red blood cell flows out this split tunnel by the first outlet flow path 328 with blood plasma or blood platelet---, this interface radially moves inwards, and this second outlet flow path 330 can make red blood cell flow out this split tunnel 310 from acquisition zone 346.During the state of underflow,---promptly when blood plasma or blood platelet flow out this split tunnel by the 3rd outlet flow path 332 with red blood cell---this interface radially outward moves, and this second outlet flow path 330 can make some blood plasma or blood platelet flow to the 3rd outlet flow path 332 from the first outlet flow path 328.
D. the 4th embodiment of blood processing chamber
Figure 23 and 24 illustrates the process chamber of representing with 360, and except some that will be described below were revised, this process chamber 360 was the same with the process chamber 300 of Figure 19-22 (same parts are represented with same Reference numeral, and do not done description in addition).Compare with the embodiment of Figure 19-22, the opening 325 of the inlet 326 shown in Figure 23-24 is set at radial position place near lateral wall part 308.Thereby, blood can with outside the tangent position of (high G) sidewall sections 308 enter in the split tunnel 310.Such position can help the separation of blood constituent, and if/or slack-off or stop by the flow rate of this inlet 326, then can avoid blood constituent to reflux.
This inlet 326 is limited by the 309C of part radially outward of lateral wall part 308.Edge 323 and this part 309C of inwall 322 are radially spaced, and are set at radial position place near the radial position of this lateral wall part 308 at the part place than the downstream of this lateral wall part 308.The fluid that flows through this inlet 326 arrives a radially outer position at this edge 323 along the path along this inwall 322, and enters in this split tunnel 310 by opening 325 then.
E. the 5th of blood processing chamber the execute example
Figure 23 A and 24A illustrate process chamber 360A, or fluid is mobile in this process chamber, and this process chamber is similar to the process chamber 360 of Figure 23 and 24, and therefore same numeral is used to describe same parts, but add letter " A " in digital back, and do not do description in addition.
Compare with 24 embodiment with Figure 23, the first flow path 344A of Figure 23 A and 24A is set at the axial positions of end wall portion 314A of the bottom of contiguous this process chamber 360A.This first flow path 344A can be limited by the surface of this end wall portion 314A.In this respect, the fluid that flows to the first flow path 344A must increase its axial length, arrives the bottom of this split tunnel 310A basically.In the downstream of dividing plate 336A, this fluid flows along the direction towards this split tunnel top from the bottom of this split tunnel 310A, thereby enters acquisition zone 346A by opening 347A.Shown in Figure 24 A, fluid occupies the sizable part at the volume of first end wall portion 314A of this split tunnel 310A bottom and this split tunnel 310A between the second end wall portion (not shown) at this split tunnel top.
Compare with 24 embodiment with Figure 23, the split tunnel 310A of Figure 23 A and 24A does not lead to the opening of outlet flow path 328A at the upstream position of dividing plate.In Figure 23 A and 24A, the opening 327A that enters the first flow path 328A is positioned at the upstream side 338A of this dividing plate 336A or is positioned at this split tunnel 310A at the downstream part slightly of this upstream side 338A.As previously described, the blood plasma that is rich in blood platelet or anaemia platelet enters this opening 327A, and can be in the junction 352 radially inwardly flows, and to leave split tunnel 310A, perhaps alternatively, flows among the second outlet flow path 330A.Fluid is communicated with between upstream side 338A that the first flow path 344A allows at this dividing plate 336A and the downstream 340A, does not lead to the outer outlet flow path of this split tunnel 310A but do not form.The red blood cell that flows through the first flow path 344A preferably leaves this split tunnel 310A by the 3rd outlet flow path 332A in dividing plate 336A downstream.
F. the 6th embodiment of blood processing chamber
Figure 25-27 illustrates another process chamber that general usefulness 370 is represented, it has interior (low G) and outer (high G) sidewall sections 372 and 374 and first end wall portion and second end wall portion (having only first end wall portion 376 to be illustrated) that is radially spaced respectively.These wall parts 372,374 and 376 limit passage 378 together.
Between relative inner radial wall 377 and 381, be limited with inlet 379.One of them interior wall 377 connects is somebody's turn to do outer (high G) wall part and the upstream and downstream end of this passage 378 is separated.Be similar to the embodiment of Figure 19-22, this interior wall limits the access road 379 of this process chamber 370, this access road 379 make fluid in abutting connection with this outside or the position of high G sidewall sections 374 can enter in the upstream extremity of this passage 378.Downstream at this passage 378 is formed with baffle plate or dividing plate 380, and this dividing plate 380 has upstream side 382 and downstream 384, and extends radially inwardly to and these madial wall part 372 spaced positions from this lateral wall part 374.This dividing plate 380 will be described in greater detail below.
In Figure 26-27, first flow path 386 (Figure 26) is communicated with between the upstream side 382 and downstream 384 of this dividing plate 380.In Figure 27, first flow path 386 be located on the bottom end wall 376 isolated and under this top end wall (not shown) isolated in the middle of axial location.Be similar to the embodiment of Figure 18-24, just extend radially outwardly than upstream portion from this lateral wall part 374 in the part 373 and 375 (Figure 26) of the lateral wall part 374 of the upstream and downstream of this dividing plate 382.The outer radial face of first flow path 386 preferably part is formed by one or more (these parts 373 and 375 are removed in Figure 27) in these radially outer parts 373 and 375 of this lateral wall part 374.The relative interior radially surface of first flow path 386 is preferably in the radial position place formation near the radial position of this outside or high G wall part 374.
Generally also be communicated with between the upstream side 382 and downstream 384 of this dividing plate 380 with 388 second flow paths of representing.As shown in figure 27, preferably, the opening 400 of second flow path 388 makes fluid can flow to this second flow path from the position than the upstream of this passage 378.Second flow path 388 is preferably limited by the surface of the second end wall portion (not shown) on the top that is placed on this process chamber shown in Figure 25-27 usually.Intermediate ends wall part 398 limits the following axial surface of this second flow path 388, and it will be discussed in further detail below.Shown in Figure 26 and 27, this second flow path 388 comprises non-radial component 387 and radial component 381 respectively.This non-radial component 387 is preferably limited by the space between the radially inner surface of madial wall part 372 and dividing plate 380.This radial component 389 is limited by the downstream 384 and the inner radial wall extension 404 of this dividing plate 380.This inner radial wall extension 404 ends at the outward flange 405 of the intermediate radial position between this madial wall part 372 and lateral wall part 374.
This process chamber 370 also comprises the formed first and second outlet flow paths 390 and 392 by the apparent surface of inner radial wall respectively.This first outlet flow path 390 is positioned at the upstream of this dividing plate 380.This second outlet flow path 392 is positioned at the downstream of this dividing plate 380.This first outlet flow path 390 and the second outlet flow path 392 extend radially inwardly from this passage 378.This first outlet flow path 390 extends radially inwardly from the opening 391 that is preferably located in madial wall part 372.This second outlet flow path 392 extends radially inwardly from opening 396.This opening 396 is communicated with an acquisition zone 394, and this acquisition zone is positioned at the downstream of this dividing plate 380 and extends to inner radial wall 377.Preferably, this first outlet flow path 390 is configured to form with the second outlet flow path 392 angle of about 45 degree, but other angle and orientation also are possible.
In Figure 26 and 27, this acquisition zone 394 is limited by end wall portion 398 at least in part at its lower boundary place, and this end wall portion 398 is spaced apart on first end wall 376 of this passage 378.The top of this acquisition zone 394 is preferably formed by the end wall portion (not shown) that is positioned at these passage 378 tops.This acquisition zone 394 also is limited between the part 375 and madial wall part 372 of this lateral wall part 374 usually.According to the interfacial position between blood plasma and the red blood cell, fluid can enter this acquisition zone 394 by first flow path 386, and also can enter by second flow path 388.Can leave and enter second outlet pathway 392 by exporting 396 from the fluid of this acquisition zone 394, so that from this passage 378, remove.
The relative position of blood plasma P and red blood cell RBC under normal condition when Figure 26 is illustrated in this interface and is positioned at the radially centre of this (low G) and outer (high G) wall part 372 and 374.Blood plasma or be rich in hematoblastic blood plasma and preferably gather by this first outlet opening 391 in flow path 390 of these dividing plate 380 upstreams.At downstream part more, also allow the part of blood plasma to flow in the opening 400 and at least a portion by this second flow path 388.The length that blood plasma flows to this second flow path 388 will depend on the interfacial position between this blood plasma and the red blood cell.For example, under normal operation, the interface between blood plasma and the red blood cell is preferably located in or near the edge 405 of this inner radial wall extension 404.During this condition, the blood plasma that flows in this second flow path 388 preferably will be retained in the radially inside of this edge 405, up to being further processed step, gather till the blood plasma thereby move this interface and allow.Allow red blood cell RBC to advance in this acquisition zone 394, and leave this passage 378 by outlet 396 of this second outlet flow path 392 by this first mobile path flow.
G. the 7th embodiment of blood processing chamber
Figure 28-30 illustrates another blood processing chamber that general usefulness 410 is represented, be similar to the embodiment of front, this process chamber 410 has the medial and lateral wall part 412 that is radially spaced and 414 and the end wall portion 416 and the relative end wall portion (not shown) that is positioned at these process chamber 410 tops that are positioned at the bottom of this process chamber 410 respectively.This medial and lateral wall part 412 and 414 and end wall portion limit passage 418 together.
In Figure 29, the interior wall 420 and 422 of radial directed has limited the inlet 424 that is communicated with this passage 418.This interior wall 422 extends to lateral wall part 414 fully, thereby the upstream and downstream end of this passage 418 is separated.Be similar to the embodiment of Figure 23-24, the opening 425 of access road 424 is set at tangent radial position place of radial position with this lateral wall part 414.Preferably, this interior wall 420 ends at the radially outer wall part 413 isolated edges 443 with this lateral wall part 414, so that the guiding fluid enters in this passage 418.This edge 443 can be positioned at the radial position near the radial position of being somebody's turn to do outer (or high G) wall part 414.
In Figure 29, be typically provided with dividing plate 426 in the downstream of this passage 418, and this dividing plate 426 comprises upstream side 428 and downstream 430 and inward flange 432 and outward flange 434 respectively.In Figure 29 and 30, this dividing plate 426 couples together this medial and lateral wall part 412 and 414 along sizable axial length of this passage.As shown in figure 30, preferably, this dividing plate 426 couples together this medial and lateral wall part 412 and 414 along the axial length of the end wall portion 416 from middle end wall portion 460 to the bottom that is positioned at this passage 418.
On this intermediate ends wall part 460, the inside and outside radial edges of this dividing plate 426 does not connect, thereby allows to flow around this dividing plate 426.As shown in figure 30, this radially inward edge 432 is spaced apart to the axial length and the madial wall part 412 of this intermediate ends wall part 460 along the top from this passage 418.This inward flange 432 partly limits the exit opening 448 of this passage 418, and this exit opening 448 is by the first outlet flow path 446.The outer radial edge 434 of this dividing plate 426 is spaced apart with the bag or the part 415 of this lateral wall part 414.The footpath that this part 415 is set at this lateral wall part 414 to the outside, this lateral wall part 414 is the upstream of this part 415.First flow path 440 is defined out between this edge 434 and this part 415, and extends to this intermediate ends wall part 460 from this top end wall part (not shown).The radial position of the outer radial edge 434 of this dividing plate 426 is preferably near the radial position that is positioned at this lateral wall part 414 of this upstream position.Below this intermediate ends wall part 460, the outer edge 432 and 434 of this dividing plate 426 fully this sidewall sections 412 and 414 and/or this part 415 between extend, between them without any at interval, as Figure 30 clearly shown in.Therefore, as shown in figure 30, dividing plate 426 should couple together by inside and outside sidewall sections 412 and 414 along sizable part of the length of this passage 418.
As shown in figure 29, this dividing plate 426 also comprises radially inner part or afterbody 436.This afterbody 436 extends radially inwardly this madial wall part 412, and ends at junction 438.This afterbody 436 and inner radial wall 442,444 and 422 limit a plurality of outlet pathways 446,450 and 454, as shown in the figure.In Figure 29,438 mutual fluid ground are communicated with the first and second outlet flow paths 446 and 450 in the junction.Preferably, lead to the opening that in the opening of this outlet pathway 446,450 shown in Figure 28-30 and 454, is not positioned at the position of these dividing plate 426 upstreams.
The opening 448 that leads to the aforesaid first outlet flow path 446 is limited between the inward flange 432 and madial wall part 412 of this dividing plate 426.This opening 448 is partly limited by this dividing plate 426, thereby and is not positioned at the upstream of this dividing plate.The second outlet flow path 450 is positioned at the more downstream part of this first outlet flow path 446, and in the upstream of this dividing plate 426 also without any opening.The opening 451 of the second outlet flow path 450 and 453 allows to be communicated with between this first and the 3rd outlet flow path 446 and 454 usually, and these openings 451 and 453 are positioned at the downstream of this dividing plate 426.As previously mentioned, according to this interfacial radial position, blood plasma can flow to this second outlet flow path 450 from the first outlet flow path 446.The 3rd outlet flow path 454 is positioned at the downstream of first and second flow paths 450 and 452, and comprises opening 456, and this opening preferably can be removed red blood cell from passage 418.First flow path 440 can be communicated with between the upstream and downstream side of this dividing plate 426, but does not allow fluid to leave this passage 418 in these dividing plate 426 upstreams.Therefore, this passage 418 is removed fluid in these dividing plate 426 upstreams without any opening from this passage 418.
This passage 418 also comprises the acquisition zone 458 (being shown in broken lines at Figure 29 and 30) that is arranged in these dividing plate 426 downstreams.This acquisition zone 458 is limited between the top and intermediate ends wall part 460 of this passage 418 usually.This acquisition zone 458 also is limited between the radial position corresponding to the part 415 of this madial wall part 412 and lateral wall part 414 usually.As contemplated by various embodiment discussed herein, the size of this acquisition zone 458 and position can change according to concrete process chamber configurations.Be similar to embodiment discussed above, this first flow path 440 and the second and the 3rd outlet flow path 450 and 454---by opening 453 and 456---can be communicated with this acquisition zone 458.
Blood plasma or be rich in hematoblastic blood plasma interfacial between blood plasma and red blood cell and radially inwardly gather.Preferably allowing this plasma flow to cross this opening 448 enters in the first outlet flow path 446 and flows out this passage 418.At this interfacial radial outside, allow red blood cell to flow through this first flow path 440 and enter in this acquisition zone 458, and leave by the 3rd outlet flow path 454.According to the interfacial position between blood plasma and the red blood cell, the second outlet flow path 450 can comprise or blood plasma, and perhaps red blood cell perhaps comprises both.During normal condition, this interface preferably remains between the radial position of the outward flange 443 of this dividing plate 426 and inward flange 432.For such condition, the second outlet flow path 450 can mainly allow blood plasma to flow on interfacial this position, but other liquid form also is possible.
H. the 8th embodiment of blood processing chamber
Figure 31-34 illustrates generally the embodiment of another blood processing chamber of representing with 410A, be similar to the embodiment of front, this process chamber 410A is similar to the process chamber of being discussed 410 in Figure 28-30, and therefore, similar parts is added letter " A " expression with same digital back.Compare with the embodiment of Figure 28-30, the process chamber 410A of Figure 31-34 comprises dividing plate 426A, and this dividing plate 426A is the afterbody shown in Figure 28-30 not.But at the downstream of this dividing plate 426A spaced apart one independent intermediate radial wall extension 436A, and this independent intermediate radial wall extension 436A has formed the part of one or more outlet flow paths.
As the embodiment of Figure 28-30, this process chamber 410A of Figure 31-34 comprises the inside and outside sidewall sections 412A and 414A and relative end wall portion that is radially spaced, and the first end wall portion 426A is shown among Figure 34.These wall parts 412A, 414A and 426A limit inlet 424A together.
Be similar to the embodiment of Figure 28-30, opening in Figure 33 and 34 or passage 448A are limited between the inward flange 432A and madial wall part 412A of this dividing plate 426A.Compare with the embodiment of Figure 28-30, this opening 448A among Figure 31-34 is communicated with the first outlet flow path 446A, but does not form the opening that leads to the first outlet flow path 446A.But the first outlet flow path 446A is set at the downstream of this dividing plate 426A, and extends radially inwardly from opening 449A, and this opening 449A is formed among this madial wall part 412A in the downstream position of this dividing plate 426A.The part of the first outlet flow path 446A is limited between this intermediate radial wall extension 436A and the interior radial wall 444A.
In radially inner position shown in Figure 32, this midfeather 436A ends at junction 438A.This junction 438A's is radially inside, and the first outlet flow path 446A is limited between inwall 442A and the 444A.At junction 438A, can flow radially outward from the fluid of the first outlet flow path 446A and to enter among the second outlet flow path 450A, or radially inwardly by the first outlet flow path, so that from this passage 418A, remove.
In Figure 32-34, the second outlet flow path 450A is limited at the downstream of this dividing plate 426A, between interior radial wall 442A and intermediate radial wall extension 436A, and comprises opening 451A (Figure 32) and 453A (Figure 34).As previously mentioned, although actual flowing will be depended on this interfacial radial position, this second outlet flow path 450A permission fluid between this first outlet flow path 446A and the 3rd outlet flow path 454A usually is communicated with.The 3rd outlet flow path 454A is limited at the downstream of this dividing plate 426A, between interior radial wall 442A and 422A, and comprises opening 456A.Therefore, shown in Figure 32-34, each outlet flow path 446A, 450A and 454A and their corresponding openings all are arranged in this passage in the downstream of this dividing plate 426A.
In Figure 32-34, acquisition zone 458A is limited at the downstream of this dividing plate 426A usually, between the top end wall (not shown) of this intermediate ends wall part 460A and this passage 418A, and be limited at usually between the radially exterior portion 415A of this madial wall part 412A and this lateral wall part 414A.The first flow path 440A is communicated with between the upstream and downstream side 428A and 430A of this dividing plate 426A, and is communicated with acquisition zone 456A fluid.In the downstream of this dividing plate 426A, one or more opening 449A, 453A and 456A can be communicated with this acquisition zone 458A according to this interfacial radial position.
As Figure 33 and 34 clearly shown in, this intermediate radial wall extension 436A ends at radially outward edge 439A.This radially outward edge 439A is arranged in this acquisition zone 458A, is in this inside and outside sidewall sections 412A and the radially middle radial position of 414A.Radially outward edge 443A in abutting connection with inwall 442A also extends among this acquisition zone 458A, and is positioned at intermediate radial position, and this position is preferably at the radial outside of the radial position of this outward flange 439A.Under normal operation, this interface is preferably located between the radial position of this edge 439A and 443A.This interface on radially blood plasma or be rich in hematoblastic blood plasma and preferably can flow among this first outlet flow path 446A, and can be at this junction 438A or along radially flowing inwards or along radially outer direction.The radially outer red blood cell of this interface preferably can flow among the 3rd outlet flow path 454A and leave this passage 418A.
I. the 9th embodiment of blood processing chamber
With reference to figure 35-38, Figure 35-38 illustrates generally another embodiment of the blood processing chamber of representing with 410B.This process chamber 410B is similar to the process chamber 410A that describes among the process chamber 410 of front and Figure 31-34, and therefore, comprises that the corresponding alphanumeric Reference numeral of letter " B " will be used to describe this process chamber 410B.Compare with the embodiment of Figure 31-34, the lateral wall part 414B of this chamber 410B does not comprise radial outside part or bag.
As previously mentioned, the first flow path 440B and passage 448B can be communicated with between this dividing plate 426B upstream and downstream side.The first flow path 440B limits between the outer radial edge 434B of this dividing plate 426B and this lateral wall part 414B vertically, and the top end wall (not shown) along radial direction from this passage 418B extends to intermediate ends wall part 460B.Between the interior radially edge 432B of this madial wall part 412B and this dividing plate 426B, limit opening or passage 448B, and it limits between the top of this passage 448B and this intermediate ends wall part 460B vertically along radial direction.Below this intermediate ends wall part 460B, this dividing plate 426B is the radical length of this passage of extend through 418B fully, couples together to the bottom of this passage thereby will be somebody's turn to do inside and outside sidewall sections 412B and 414B one road.
Shown in Figure 36 and 37, acquisition zone 458B is communicated with two outlet flow path 446B and 454B by corresponding opening 449B and 456B, thereby preferably allows to remove respectively from passage 418B blood plasma and red blood cell.This acquisition zone 458B also comprises intermediate radial wall extension part 436B, and the downstream of this intermediate radial wall extension part 436B and this dividing plate 426B is spaced apart, and spaced apart with the upstream of this outlet flow path 446B and 454B.Compare with the embodiment of Figure 31-34, this intermediate radial wall extension part 436B shown in Figure 35-38 is extending radially inwardly at this madial wall part 412B not.As Figure 36 clearly shown in, this intermediate radial wall extension part 436B has outer edge 438B and 439B respectively, these edges are preferably spaced apart with corresponding inside and outside sidewall sections 412B and 414B.In Figure 37 and 38, though mobile requirement according to process, this radially other position of wall extension part 436B also be possible, but radially wall extension part 436B is preferably near this madial wall part 412B location for this, and this can allow priming operation (priming) part of this process chamber 410B.
In Figure 37, under normal operation, promptly under not underflow condition or overflow state, the interface between blood plasma and the red blood cell preferably roughly is located between this edge 439B and the 443B.Preferably can flow to the opening 449B at the radially inner blood plasma of interface, and leave, so that from this passage 418B, remove blood plasma by outlet flow path 446B from acquisition zone 458B.Preferably can flow among the opening 456B and leave at the radially outer red blood cell of interface, so that from this passage 418B, remove red blood cell by outlet flow path 454B.
J. the tenth embodiment of blood processing chamber
Figure 39-42B illustrates another example of the blood processing chamber that general usefulness 500 represents, and wherein Figure 41 is illustrated in the path that these process chamber 500 inner bloods flow.As the embodiment that had discussed the front, this process chamber 500 comprises inside and outside sidewall sections 502 and 504 and relative end wall portion (end wall portion 506 shown in Figure 39) respectively, and these wall parts limit passage 508 together.This lateral wall part 504 comprises part 505 (Figure 39 and 40A) radially outward, this radially outward part 505 be positioned at radial outside than this lateral wall part 504 of upstream position.
The interior radial wall 510 and 512 of two radial directed limits an inlet, and it represents with 514 that generally this inlet 514 stretches out from axle sleeve 501.As Figure 42 A clearly shown in, this inlet 514 comprises the several sections 546,548,550 and 552 that usually is provided with along different directions.First 546 along the extending radially outwardly of axle sleeve 501, and is partly limited by top end wall part (not shown) between inner radial wall 510 and 512.Second portion 548 is axially guided by the end from this first 546, and is limited between the top end wall part and middle axial location of this process chamber 500.Third part 550 is radially guided by the end from this second portion 548, and radially departs from the first 546 of this inlet 514.The other end of this third part 550 is limited at the radially outer position near this lateral wall part 504.The 4th part 552 is configured to substantially perpendicular to this third part 550, and by the upstream extremity guiding towards this passage 508, so that make fluid can enter this passage 508.In Figure 41, the fluid path that is defined as by the 4th part 552 is parallel to the fluid path that is limited by this passage 508 substantially.As shown in figure 41, fluid enters this passage 508 one with this top end wall section axial spaced positions place that is positioned at the top of this passage 508.
Just as in the previous embodiments, the passage 508 among Figure 39 comprises the dividing plate that general usefulness 516 is represented, it has upstream side 518 and downstream 520.This dividing plate 516 is approximately perpendicular to lateral wall part 504 and extends.In Figure 40 A and 42B, the outward flange of dividing plate 516 522 is spaced apart with the part radially outward 505 on the intermediate ends wall part 536, thereby and limit first flow path 524 (as Figure 40 A and 41 clearly shown in).On intermediate ends wall part 536, first flow path 524 allows to flow around the outer radial edge 522 of dividing plate 516.This edge 522 has the radial position of the radial position of close lateral wall part 505.First flow path 524 preferably is limited between the top end wall part (not shown) and intermediate ends wall part 536 of this passage 508 vertically, and this centre end wall 536 is spaced apart with the bottom end wall part 506 of this passage.Below this intermediate ends wall part 536, this outer radial edge 522 couples together this lateral wall part 504 or its part 505, so that do not allow usually to flow around this dividing plate.
In Figure 40,40A and 42B, dividing plate 516 preferably extends radially inwardly to the radially inner radial position of madial wall part 502.This dividing plate 516 has formed the separator between the first outlet flow path 526 and the second outlet flow path 528.Shown in Figure 40 and 40A, the first outlet flow path 526 is limited between the upstream side 518 and inner radial wall 530 of dividing plate 516.Be provided with the opening that leads to this path or export 532 in madial wall part 502, to allow flowing out this passage in the upstream of this dividing plate 516.The second outlet flow path 528 is set at the downstream of dividing plate 516, and comprises opening 534.This second outlet flow path 528 is limited between this dividing plate 516 and the inner radial wall 512.The footpath of this inner radial wall 512 edge 513 to the outside is set at inside and outside sidewall sections 502 and 504 middle radial positions.Preferably, this edge 513 radially inside at the outward flange 522 of dividing plate 516.
As Figure 40 A clearly shown in, be provided with acquisition zone 538 in the downstream of dividing plate.This acquisition zone preferably is limited between this top end wall part (not shown) and the intermediate ends wall part 536 (also seeing Figure 42 B) vertically, and this intermediate ends wall part 536 is spaced apart with bottom end wall part 506.This acquisition zone 538 is communicated with first flow path, 524 fluids.In Figure 40 A, preferably, this acquisition zone 538 is formed in its radial extension between the radially outer part 505 of madial wall part 502 and this lateral wall part 504.Opening 534 is communicated with acquisition zone 538, so that a kind of and multiple fluid composition can flow, preferably, red blood cell leaves by outlet flow path 528, and is used for removing from passage 508.
In Figure 40 A, this acquisition zone 538 also comprises the radial passage 540 that is positioned at inner radial wall 512 right sides.This passage 540 is limited between this interior radial wall 512 and the extension 542, and this extension 542 extends radially inwardly from lateral wall part 504.This passage 540 extends to madial wall part 502, and it is communicated with that part of of passage 508 on Figure 40 A right side by non-radial passage 544 herein.This extension 542 ends at the radial position in the middle of this inside and outside sidewall sections 502 and 504, and preferably ends at the radially inner radial position at this edge 513.Therefore, this extension 542 makes non-radial passage 544 be positioned the position of contiguous this madial wall part 502.Preferably, radial passage and non-radial passage 540 and 544 boths are limited between top end wall part (not shown) and the intermediate ends wall part 536 vertically.
Shown in Figure 40 A, passage 540 and 544 allows to be communicated with between the upstream and downstream end of this passage 508 usually.In this respect, passage 544 preferably makes blood plasma flow to the acquisition zone 538 from that part of of passage 508 on these passage 544 right sides.As shown in figure 41, blood plasma flows to the left side of this extension 542.Preferably, when the approximate radial position of interface between edge 513 and 522, blood plasma flows in the acquisition zone 538.As previously mentioned, Figure 42 A illustrates inlet 514 and its each several part 546,548,550 and 552, and these parts are configured to so that walk around around the path of this passage 540 and 544 and enter in this passage 508.Intake section 552 can help avoid whole blood in the location of madial wall part 502 or other fluid flow in this acquisition zone before composition has an opportunity to separate fully.
In Figure 40 A, blood plasma preferably leaves passage 508 by first opening 532 that exports flow path 526 in 516 left sides of the dividing plate among Figure 40 A.Also allow blood plasma to flow in the acquisition zone 538 on these dividing plate 516 right sides among Figure 40 A, so that keep the blood plasma volume on the interface radial position by passage 544.Preferably, red blood cell leaves by the second outlet flow path 528.Under normal operation, the interface in this acquisition zone 538 is preferably located in the outward flange 522 of dividing plate 516 and between the edge 513 that forms on the interior wall 512.Blood plasma is supplied with by passage 544, with at least a portion of the volume that is filled in the radially inner acquisition zone 538 of this interface.The radial position at this edge 513 does not preferably allow blood plasma to flow to the second outlet flow path 528.
III. the platelets gathering process of utilizing this system to concentrate
Can adopt above-described any embodiment to carry out various biofluid gatherer processes, for example sampled plasma process, two red blood cell gatherer process and platelets gathering process and other gatherer process.This process can be carried out with aforesaid blood separating mechanism 14 and controller 16 with blood flow utensil 12.Blood separating chamber shown in Figure 43-48 generally will represent with Reference numeral 18, and it can comprise in the previous embodiment structure of any.
Though will describe several platelets gathering processes below, and be to be understood that the foregoing description can be used for other gatherer process, and can adopt more than one gatherer process.By way of example rather than ways to restrain, in being incorporated into this above-mentioned patent for reference or patent application one of them has described typical blood plasma and two red blood cell gatherer processes at least.In addition, any embodiment described herein amount that can be used for allowing according to concerned countries is gathered more than one blood constituent.Though will be described below the collection of being rich in hematoblastic concentrate, considered with any in these methods of its extensive interpretation to comprise other biofluid composition and other blood constituent.
A. be used for the recirculation of platelets gathering
Figure 43-45 schematically illustrates the method that is used for platelets gathering.In Figure 43, with fluid composition, be preferably whole blood pump and send in the process chamber 18.This blood can or from the blood source, preferably flow to this process chamber 18 from the contributor, perhaps can be from will temporarily leaving in wherein so that flow to this process chamber 18 by what process chamber was handled at goods (in-process) container 158 subsequently from the blood in blood source.Allow whole blood WB to flow, for example, enter this process chamber 18 by the inlet flowline by means of carrying out pumping at goods (in-process) pump IPP.
In this process chamber 18, separate according to carry out fluid composition as the density among Figure 11.As mentioned above, the detail of separation is disclosed in " The physics of ContinuousFlow Centrifugal Sell Separation " literary composition of Brown, sees Artificial Organ, 13 (1): 4-20 (1998).Composition such as the higher density of red blood cell RBC is pushed near outer or high G sidewall sections, and is pushed near interior or low G sidewall sections such as the more low-density composition of the blood plasma of anaemia platelet.In Figure 11, the interface between red blood cell and the blood plasma comprises buffy coat, and this buffy coat comprises at least a portion blood platelet and white blood corpuscle, but interfacial composition will change along with the detailed process that is adopted.
Make after interface can form through time enough, according to the needs of process, by corresponding outlet 104 or 106, can be from interfacial any side, or gather fluid individually from its both sides.For example, the blood plasma PPP of some anaemia platelets can radially inwardly gather at interface by outlet 106, and enters in the blood plasma collection container 160.And some red blood cell RBC can radially outward gather at interface by outlet 104, and flow to red blood cell collection container 162.The blood platelet that the above-mentioned dividing plate that is arranged in top process chamber preferably allows to be included in buffy coat is assembled at this blood plasma or red blood cell gatherer process, but hematoblastic collection does not begin as yet.
Before gathering blood platelet, preferably underflow condition is put on fluid composition.This underflow condition is shown among Figure 13.Optical pickocff 148 detects the part of blood plasma and leaves pipe 104, and this pipe has the red blood cell that leaves by it usually.This underflow condition is rule of thumb determined according to the optical transmittance of the light that passes through the composition in this outlet 104.Data transaction haematoblast specific volume with optical pickocff.The hematocrit of outlet 104 reduce to detect underflow condition.Interfacial radial position during with normal acquisition operations (Figure 11) is compared, and facilitates underflow condition that interface can be forced to radially outward.Underflow condition can move to red blood cell in the red blood cell collection container 162, in the scope of the hematocrit of the fluid that in this process chamber, is produced between percent 20 to percent 40 till.
In case obtain desirable hematocrit, preferably the fluid in this process chamber 18 remains in the desirable hematocrit scope.For example, flowing of blood plasma can stop, and flow in the blood plasma collection container 160 preventing, and also can stop from the flowing of red blood cell of this process chamber 18.This flowing can and/or stop to realize such as one or more pumps of blood plasma pump PP by operation valve platform 30.Though preferably at goods (in-process) pump IPP with low flow rate work, it can continuous firing.
This method is further comprising the steps of: the fluid composition that reconfigures the separation in this process chamber 18.Preferably, reconfigure by carrying out along rotating this process chamber with counter clockwise direction clockwise.Preferably, this process chamber 18 is alternately to rotate one or many along clockwise and counter clockwise direction.Reconfigure the uniform blood mixture fluid that step preferably obtains comprising blood plasma, red blood cell, blood platelet and white blood corpuscle, and have the hematocrit of approximate as previously mentioned process chamber.Though this time period can change, preferably reconfigure step and continue about one to three minute.The speed of rotation of this process chamber when the speed of rotation of any direction preferably is significantly smaller than this composition initially-separate for example, change in the scope of (RPM) at about per minute 300 to 600, but other the speed of rotation also is possible.Though should be noted that can also with an omega or its certain the combination, used here angular speed is two omegas normally.
Enough reconfigure the time cycle after, rotor is reset then, rotates this process chamber with consistent all the time direction, so that the fluid in this process chamber is directed to outlet 104 and 106 from inlet tube 102 usually.Though the concrete speed of rotor can change, this speed can be that per minute 2500 changes.Can form the interface between blood plasma and the red blood cell again.Preferably, do not begin to gather blood plasma and red blood cell, till interface forms again after time enough from this process chamber 18.
After interface formed again, blood plasma and at goods (in-process) pump operation was to extract blood plasma and to extract red blood cell by outlet 104 from this interfacial radial outside from this interfacial radially inner side by outlet 106.As shown in figure 44, two kinds of compositions all pass through inlet tube 102 and transfer back, are used for circulating again by this process chamber.In cycle period again, do not have in the collected container 160 of blood plasma and red blood cell and 162.During recirculation, the hematoblastic concentration in the blood plasma increases usually, and becomes from interfacial blood platelet and to be suspended in the blood plasma.The recirculation of two kinds of compositions lasts till that optical pickocff 146 detects is rich in till the hematoblastic blood plasma, and this is rich in hematoblastic blood plasma and has desirable PC, and visually red blood cell content is lower.As mentioned above, the hematocrit of recirculation mixture is greatly between percent 20 to percent 40.Recirculation also can change, so that only recycle a kind of in these compositions as required, or blood plasma, or red blood cell.
During recirculation, although according to the actual conditions of system, can adopt other the pump flow speed ratio rate at goods pump IPP and blood plasma pump PP, preferably the pump flow speed ratio rate at goods pump IPP and blood plasma pump PP is 60/40.Recirculation can also increase the concentration of white blood corpuscle WBC, is rich in interface between hematoblastic blood plasma and the red blood cell with setting.Have found that this pumping ratio can directly influence the number of white blood corpuscle WBC, this is rich in infringement the collecting efficiency of hematoblastic blood plasma PRP and whole PC.By way of example rather than the mode of restriction, Figure 45 A and 45B illustrate the concentration (Figure 45 B) that the fluid of being gathered has higher PC (Figure 45 A) and lower white blood corpuscle WBC.In Figure 45 A and 45B, gather this fluid from the process chamber 18 of surface area with 120cm2, this process chamber is with the omega speed operation of about 1250RPM, and the hematocrit of this process chamber is about 25%.For the hematocrit of different chamber surfaces area, centrifugal speed and process chamber, can develop other collecting efficiency.
The recirculation of being rich in hematoblastic blood plasma PRP can continue a few minutes, preferably continue about 2 to 4 minutes, but this scope can change according to detailed process., be rich in hematoblastic blood plasma PRP and be collected in the platelet concentrate container 161, as shown in figure 45 after the cycle at enough RCTs by outlet 106.And in Figure 45, the blood plasma PPP of anaemia platelet has substituted the fluid volume loss in this process chamber 18 that causes owing to the collection of being rich in hematoblastic blood plasma PRP.Though described the collection of being rich in hematoblastic blood plasma PRP in the above, this method still can be used for the blood plasma PPP of anaemia platelet and/or the collection of red blood cell.
Various modifications to said method are possible.A kind of modification is included in and operates in goods pump IPP at least between two kinds of different pump rates, to realize reconfiguring of blood constituent.For example, can be with first flow rate pump fluid into this process chamber 18 at the goods pump, rotation clockwise or counterclockwise simultaneously, and repeat rotation with second flow rate then along either direction.For example, can reduce centrifugal force, wherein use more than a kind of flow rate by reducing rotary speed.
The another kind of modification comprises: reconfiguring manipulate blood plasma pump PP.Blood plasma is gathered by outlet 106, and flows in product 158.Simultaneously, inlet tube 102 places flow through utilization and oppositely at goods pump IPP, thereby also flow to this in product 158 from the fluid of process chamber 18 by inlet tube 102.Can flow back to this process chamber 18 by inlet tube 102 then at fluid 158 li of product.Therefore, fluid composition is mixed together in the outside of this process chamber 18, and reenters then in this process chamber.
In acquisition phase, the pumping ratio between goods pump IPP and blood plasma pump PP can also be modified as and have different ratios on the time different in this process.The another kind of said method revised comprise: after hematoblastic blood plasma is rich in collection, adds solution with blood platelet and change volume in this process chamber 18.
In addition, can revise the time span of these process chamber 18 recirculation of turnover.For example, the time cycle that increases recirculation can force more white blood corpuscle radially outward to arrive this interface place, so that the hematoblastic blood plasma PRP that is rich in that is gathered has less white blood cell count.By way of example rather than ways to restrain, Figure 45 C is illustrated in and gathers blood platelet and the white blood cell count that is rich in during the hematoblastic blood plasma PRP recirculation.In Figure 45 C, first sample occurs in that blood plasma pump is reset so that 15 seconds after recycling, and thereafter approximately per minute get sample one time.Sample #5 occur in begin be rich in hematoblastic blood plasma PRP collect in the platelet concentrate container 161 after 15 seconds.During recirculation the concentration of white blood corpuscle descends, and each sample is approximate during first a few minutes reduces by half.The result.The lengthening in RCT cycle is deposited in more white blood corpuscle and is rich in outside the hematoblastic blood plasma, and therefore produces the platelet concentrate that white blood corpuscle reduces, and compares during with the recirculation beginning, and it has considerably less white blood corpuscle.Other modification also is possible.
B. reduce to be used for the centrifugal force of platelets gathering
The another kind of method of platelets gathering comprises and reduces centrifugal force, so that separate and gather desirable fluid from this process chamber.This fluid is preferably and is rich in hematoblastic blood plasma PRP, the composition that it provides blood platelet and has the blood plasma of higher PC.
Be similar to the method for aforesaid Figure 43-45, this method may further comprise the steps: with fluid, preferably whole blood is introduced wherein in any one of aforementioned processing chamber.Preferably rotate this process chamber and apply centrifugal force, cause separation shown in Figure 11 by axis around this process chamber.Blood platelet and white blood corpuscle are deposited in the interface or buffy coat between red blood cell and the blood plasma usually.In this interface, at least some separation can occur between blood platelet and the white blood corpuscle according to density.In this respect, hematoblastic thin layer can be positioned at contiguous blood plasma.By way of example rather than ways to restrain, the rotary speed in preferably about 4500 to 5000RPM scopes produces the thick platelet layer of about 1mm to 3mm in this interface, but can also use other speed.
After the initially-separate, reduce centrifugal force.This reducing of centrifugal force preferably realized by the rotary speed that reduces this process chamber.The reducing of centrifugal force preferably is enough to cause the expansion that is present in the platelet layer in the interface, thereby also causes this interfacial expansion, as shown in figure 46.By way of example rather than ways to restrain, the rotary speed that is preferably 2500RPM provides about 4mm to 6mm thick platelet layer.
During the interface thickening, wish from this interface or buffy coat, to gather blood platelet as much as possible, as being rich in hematoblastic blood plasma.By way of example rather than the mode of restriction, collection can be by radially moving inward expansion towards madial wall part or low G wall interface, the overflow state that is similar to Figure 12 with formation are carried out.In this respect, optical pickocff 146 is monitored the hematoblastic appearance in the outlet 106 optically.At this moment, when in outlet 106, detecting the blood platelet of enough concentration, allow fluid to flow to platelets gathering container 161 from this process chamber 18.At this moment before, can flow in the blood plasma collection container 160 from the fluid of process chamber 18.
Modification to this method also is possible, and this modification is not limited to the concrete structure that illustrates and describe here.In addition, this method can make up with any other method described here.In gatherer process, hematoblastic removing can be carried out twice or repeatedly.With the combination of this method in also can carry out other gatherer process, for example dividing comes gathers the blood plasma and/or the red blood cell of anaemia platelet.
C. repeat to be formed for the interface of platelets gathering
This method is provided for gathering fluid from this interfacial side, and this interface is formed again, is preferably used for carrying out another collection of this fluid.Be similar to preceding method discussed above, this method is preferably introduced whole blood this process chamber, and according to density this separation of whole blood is become some kinds of compositions, as shown in figure 11.Interface or buffy coat between blood plasma and red blood cell the intermediate radial position place and contain blood platelet.
By facilitating overflow state, thereby force this interface, gather the blood platelet in this interface, as shown in figure 12 radially upward to madial wall part or low G wall.As previously mentioned, in outlet 16, blood platelet is monitored optically by optical pickocff 146, and when this optical pickocff 146 detects in the blood plasma blood platelet that exists and has enough concentration, will be rich in hematoblastic blood plasma PRP and transfer in the platelets gathering container 161.
, stop to gather after the cycle at predetermined acquisition time, and allow this interface to turn back to its previous intermediate radial position place, as shown in figure 11.In this position, this interface forms again, thereby may be moves or the blood platelet that disperses can be returned to this interface from this interface.After the time enough,, can adopt another overflow state in the past, so that allow this interface radially to move inward, and make and more be rich in hematoblastic blood plasma PRP by outlet 106 and collected so that form interface again.
In one modification, remove the step that is rich in hematoblastic blood plasma PRP and can repeat at least twice, and in each continuous removing between the incident, this interface can form again.In another kind was revised, this method can make up with any other method described herein.By way of example rather than the mode of restriction, this method can reduce centrifugal force combination with aforesaid.This method can also make up with blood plasma that separates the anaemia platelet and/or red blood cell collection.
IV. utilize the gatherer process of red blood cell/blood plasma that this system makes up
The red blood cell that aforesaid any process chamber can also be used to making up and the gatherer process of blood plasma---this gatherer process branch comes and gathers red blood cell and blood plasma---replace or except the gatherer process of above-mentioned platelet concentrate.Therefore, this system and its parts can be revised in case of necessity, and to carry out the step of this process, this will be described in greater detail below.
A. first extract circulation
As shown in figure 47, blood source BS is connected by fluid ground, so that blood can be handled by blood separating mechanism 10 (Fig. 1) and its mobile utensil 12 (Fig. 4-6).The fluid intake that blood enters this mobile utensil schematically is shown in Figure 47.This blood source BS can be contributor or other people under test, as shown in the figure, or is connected in other blood source of this blood separating mechanism.The contributor can for example be connected in blood separating mechanism by the arm that intravenous needle 128 is inserted the contributor.Whole blood can flow to this flow line 126 (seeing Fig. 5 and Fig. 6), and here by corresponding flow line 152, whole blood can mix with the anti-coagulants from anti-coagulants container 150, as shown in figure 47.
After blood source BS was connected in this blood separating mechanism, whole blood WB was preferably by being flowed by the suitable flow duct that this system guided, to fill this process chamber 18.This process chamber 18 is estimated to be ready for blood processing by one and a plurality of pre-gatherer processes, this in advance gatherer process for example remove the air of this process chamber and be this process chamber priming operation with method saliferous and/or that other is suitable.Whole blood enters this process chamber 18 by inlet flow duct 102, till this process chamber 18 is filled.Also extract whole blood, and it is left in the intermediate receptacle 158 temporarily, so that handle by this process chamber 18 subsequently from blood source BS.The volume of the whole blood that extracts from blood source BS is measured by for example weight scale 62 of this system (Fig. 3-6).Whole blood is gathered in continuation from the blood source, till reaching certain predetermined botal blood volume, perhaps so that as described belowly can partly or entirely return circulation.By way of example rather than the mode of restriction, in the red blood cell and sampled plasma process of combination, this process can be gathered the whole blood of about two units or 800 milliliters.Other whole blood collection capacity is possible, and this depends on aim parameter and the component type of being gathered.
Be similar to aforesaid method, handle the whole blood in this process chamber 18, allow to become its composition, as shown in figure 11 according to density separation.After enough processing times, remove fluid from this interfacial each side.Blood plasma P is removed from this interfacial side.Red blood cell is removed from this interfacial opposite side.In Figure 47, blood plasma P leaves this process chamber 18 by outlet 106, and red blood cell concentrate RBC then leaves outlet 104.
The first fluid composition and second fluid composition 2, preferably blood plasma and red blood cell are removed from this process chamber, enter in their corresponding collection containers 160 and 162.The volume of every kind of fluid composition being gathered in container 160 and 162 is also measured in whole collection circulation.In this process chamber 18 these become divisional processing and gather preferably and continue, till the volume in one of them at least of fluid acquisition container 160 and 162 reaches predetermined minimum threshold, but before collecting the long-pending at least a fluid composition of target population.When one of them of the volume of collection container 160 and 162 reached this predetermined minimum threshold, this blood separating mechanism was configured to all or part of at least a in these blood constituents of returning.
B. return circulation
In Figure 48, in these fluid compositions at least a, preferably red blood cell RBC is returned to the contributor.In returning circulation, flow in this process chamber 18 and processed from the whole blood of intermediate receptacle 158.The separation of composition and collection preferably continue in this process chamber 18, but if desired, then at least a composition can return to the blood source.In Figure 48, the red blood cell RBC that leaves this process chamber 18 is returned to the contributor.In this process in the red blood cell of collected this point all or part of can return to the contributor, and the amount of returning can depend on the detailed process that is adopted.
Figure 49 illustrates the red blood cell of combination and the more detailed description of blood plasma process, and wherein all red blood cells all are returned to the contributor " last returns ", and all blood plasma is existed in this system.Though the blood plasma volume in the blood plasma collection container 160 can be retained in this system, according to the requirement of process, part blood plasma can also be returned to the contributor.
After at least a desirable volume of these fluid compositions has turned back to the blood source, return circulation and finish.The circulation of returning does not in addition preferably begin, because these circulations will increase the time during the blood source must be connected in separator.
C. second extract circulation
After returning circulation, extract other whole blood and handling as previously mentioned and as shown in figure 47 from this blood source.The botal blood volume that extracts from blood source BS in extraction circulation for the second time depends on predicted value.This value preferably depends on blood plasma and the volume of red blood cell and the specific volume of gathering that leaves the red blood cell of this process chamber 18 in collection circulation for the first time.The volume of the red blood cell in the volume data of the blood plasma in the container 160 and the container 162 preferably extracts in the circulation in the whole first time and is monitored, for example monitor by LOAD CELLS, and, before being turned back to blood source BS, measure these compositions extracting cycle end for the first time.Hematocrit in this process chamber 18 is determined optically by the sensor 148 (Figure 11) that is arranged in this outlet 104.This system utilizes these bulking values and hematocrite value, rule of thumb calculates and must extract how many whole bloods from this blood source BS, with the final volume of the target that reaches at least a or two kinds of fluid compositions.In the calculating of this system, this system considers that also the blood plasma gathered or the volume of red blood cell are to be saved or to return to blood source BS.
In the example of Figure 49, it approximately is 160 milliliters gathering the blood plasma volume of being preserved after the circulation for the first time.The volume of the red blood cell of preserving is 0 milliliter or approaches zero.The target volume of blood plasma and red blood cell is respectively about 400 milliliters and 240 milliliters.Extract, be about 480 milliliters from this contributor with the other whole blood that reaches by these determined these target volumes of system.Therefore, this is to gather the volume of whole blood that must extract in the circulation for the second time.Under different bulking values and hematocrite value, other volume will be conspicuous.
B. break away from the cycle of treatment after connecting
After the volume of whole blood that extracts scheduled volume, blood source BS or contributor can break away from blood separating mechanism and be connected.Except blood source BS and blood separating mechanism break away from is connected, as top among Figure 47 for extract for the first time circulation described, the processing of repetition whole blood.After the contributor broke away from connection, the processing of whole blood continued, thereby has reduced the real time that the contributor is connected with blood separating mechanism.Therefore, blood source BS is connected in and measures less than the total time quantum of whole blood during being gathered and handle by this blood separating mechanism the total time that is consumed during the blood separating mechanism.
By way of example rather than the restriction mode, Figure 49 illustrates the gatherer process that is used for about 800 milliliters of whole bloods, total time of this process is about 21 minutes.The red blood cell of the blood plasma that this process collection is about 400 milliliters and about 240 milliliters (or units)---and remaining red blood cell has returned to the contributor returning cycle period.The contributor is connected in the total time of this blood separating mechanism less than total processing time---promptly less than 21 minutes---because the contributor can be connected with this blood separating mechanism disconnection after last returning.In Figure 49, the total time that the contributor is connected in blood separating mechanism may approximately be 14 minutes.Other total time and contributor's connect hours is possible, and can depend on the enabling objective thing.
Preferably, at least two kinds of compositions, for example blood plasma and red blood cell are removed in vain and are stored in their corresponding collection containers 160 and 162, and be until the total target volume that reaches at least a blood constituent, perhaps processed until all blood.The processing that can carry out other according to above-mentioned any method or any gatherer process with separate.For example, can adopt above-mentioned any or several different methods to gather platelet concentrate.Can be according to any aforesaid method with the blood plasma of anaemia platelet from the interface blood platelet that suspends again.Perhaps, in order to gather blood platelet, can utilize blood platelet to add solution or PAS.Therefore, this method can also combine with above-mentioned any method, to gather at least two kinds of blood constituents, blood plasma and red blood cell and platelet concentrate.Collection capacity will change according to the collection limit of concrete national regulation.
From top explanation as can be seen, the present invention has some different aspects and feature, and these aspects and feature are not limited to concrete process chamber shown in the accompanying drawing or the detailed process of being discussed.The variation of these features can be embodied in other structure of other process that is used for carrying out blood separation, processing or gathers.

Claims (100)

1. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and end wall portion, this split tunnel has an axial length,
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the passage in the middle of these two sidewall sections and have the dividing plate in upstream side and downstream;
Be communicated with the upstream side of this dividing plate and first flow path in downstream;
Be positioned at this dividing plate the downstream, with this first acquisition zone that path flow body is communicated with that flows, this acquisition zone is at least in part by limiting with the axially spaced end wall branch of the described end wall portion of this split tunnel; And
First opening that is communicated with this acquisition zone and second opening are used to allow one or more fluid compositions to flow from this acquisition zone.
2. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and end wall portion, this split tunnel has an axial length;
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the middle of the passage of these two sidewall sections and have the dividing plate in upstream side and downstream;
First flow path that is communicated with this dividing plate upstream side and downstream;
This outer wall section comprises a near part that is positioned at this dividing plate, and this part of this outer wall section is positioned at the outside of outer wall section of the split tunnel of this part upstream side diametrically.
3. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and end wall portion, this split tunnel has an axial length;
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the middle of the passage of these two sidewall sections and have the dividing plate in upstream side and downstream, this dividing plate extends to this outer wall section, and meets at this outer wall section along sizable part of the axial length of this split tunnel; And
Be communicated with the upstream side of this dividing plate and first flow path in downstream.
4. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and end wall portion, this split tunnel has an axial length;
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the middle of the passage of these two sidewall sections and have the dividing plate in upstream side and downstream, this dividing plate extends to inside radial position place of this madial wall part; And
Be communicated with the upstream side of this dividing plate and first flow path in downstream.
5. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and end wall portion, this split tunnel has an axial length;
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the middle of the passage of these two sidewall sections and have the dividing plate in upstream side and downstream;
First flow path that is communicated with this dividing plate upstream side and downstream;
First outlet pathway that is communicated with the split tunnel of this dividing plate upstream;
Second outlet pathway that is communicated with the split tunnel in this dividing plate downstream;
These two outlet pathways merge together in the radially inner position of this inner wall section.
6. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and end wall portion, this split tunnel has an axial length;
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the middle of the passage of these two sidewall sections and have the dividing plate in upstream side and downstream;
Be communicated with the upstream side of this dividing plate and first flow path between the downstream;
Be positioned at a plurality of exit openings of the split tunnel in this dividing plate downstream, and in the upstream of this dividing plate, this split tunnel there is not exit opening.
7. one kind is used for rotating split tunnel with the separating bio fluid around axis, comprising:
The madial wall part that is radially spaced and lateral wall part and relative first end wall portion and second end wall portion, this split tunnel has an axial length;
In order to fluid is delivered into the inlet in this split tunnel;
Be positioned in the middle of the passage of these two sidewall sections and have the dividing plate in upstream side and downstream, this baffle wall extends to this outer wall section;
Be communicated with the upstream side of this dividing plate and first flow path between the downstream, this first flow path and this first end wall portion are spaced apart;
Be communicated with the upstream side of this dividing plate and second flow path in downstream, this second flow path is limited by the surface of this second end wall portion.
8. split tunnel as claimed in claim 1, wherein said dividing plate extend to inside radial position place of described inner wall section.
9. split tunnel as claimed in claim 1, wherein said dividing plate meets at this outer wall section along sizable part of the axial length of this split tunnel.
10. split tunnel as claimed in claim 1, wherein said dividing plate extend between the radial position of described inside and outside wall part at least.
11. split tunnel as claimed in claim 1, wherein said outer wall section comprise a near part that is positioned at the described dividing plate, this part of this outer wall section is positioned at the outside of outer wall section of the split tunnel of this part upstream side diametrically.
12. split tunnel as claimed in claim 11, wherein said dividing plate converges mutually with the described part that is positioned at the outside diametrically of described outer wall section.
13. split tunnel as claimed in claim 1, the surface of wherein said first flow path comprises described outer wall section.
14. split tunnel as claimed in claim 1 wherein also comprises first outlet pathway that is communicated with the split tunnel of the upstream of described dividing plate; Second outlet pathway that is communicated with split tunnel with this dividing plate downstream.
15. split tunnel as claimed in claim 14, wherein said first and second outlet pathways converge mutually in a radially inner position of described inner wall section.
16. split tunnel as claimed in claim 14 wherein also comprises and first of the downstream of described dividing plate the 3rd outlet pathway that the path flow body is communicated with that flows, thereby can remove the fluid composition that is guided by this first flow path from this split tunnel.
17. split tunnel as claimed in claim 1 wherein also is included in a plurality of exit openings of this split tunnel in the downstream of described dividing plate, and at the upstream end of this split tunnel, this split tunnel does not have exit opening.
18. split tunnel as claimed in claim 1 wherein also comprises and the described first end wall portion second opposed end wall part, and wherein said first flow path and this first end wall portion are spaced apart.
19. split tunnel as claimed in claim 18 wherein also comprises the upstream side that is communicated with described dividing plate and second flow path in downstream, this second flow path is limited by the surface institute of described second end wall portion.
Enough distances 20. split tunnel as claimed in claim 1, wherein said dividing plate extend radially outwardly, thus the fluid composition that is directed in this first flow path into comprises red blood cell.
21. split tunnel as claimed in claim 14, wherein said dividing plate extends radially inwardly enough distances, thus be directed into this first outlet pathway and second outlet pathway at least one of them fluid composition comprise blood plasma.
22. split tunnel as claimed in claim 14, wherein said dividing plate be along extending radially inwardly enough distances, thus be directed into this first outlet pathway and second outlet pathway at least one of them fluid composition comprise blood platelet.
23. split tunnel as claimed in claim 2, wherein also be included in the downstream of described dividing plate and the acquisition zone that the described first mobile path flow body is communicated with, this acquisition zone is at least in part by limiting with the axially spaced end wall branch of the described end wall portion of this split tunnel; And
First opening that is communicated with this acquisition zone and second opening are used to allow one or more fluid compositions to flow out from this acquisition zone.
24. split tunnel as claimed in claim 2, wherein said dividing plate extend to the inside radial position of described inner wall section.
25. split tunnel as claimed in claim 2, wherein said dividing plate meets at described outer wall section along sizable part of the axial length of this split tunnel.
26. split tunnel as claimed in claim 2, wherein said dividing plate extend between the radial position of described inside and outside wall part at least.
27. split tunnel as claimed in claim 2, the surface of wherein said first flow path comprises described outer wall section.
28. split tunnel as claimed in claim 2 wherein also comprises first outlet pathway that is communicated with the split tunnel of described dividing plate upstream; Second outlet pathway that is communicated with split tunnel with this dividing plate downstream.
29. split tunnel as claimed in claim 28, wherein said first outlet pathway converges in a radially inner position of described inner wall section mutually with second outlet pathway.
30. split tunnel as claimed in claim 28 wherein also comprises with the described first mobile path flow body in described dividing plate downstream being communicated with the 3rd outlet pathway, thereby can remove the fluid composition that is guided by this first flow path from this split tunnel.
31. split tunnel as claimed in claim 2 wherein also is included in a plurality of exit openings of this split tunnel in the downstream of described dividing plate, and at the upstream end of this split tunnel, this split tunnel does not have exit opening.
32. split tunnel as claimed in claim 2 wherein also comprises and the described first end wall portion second opposed end wall part, and described first flow path and this first end wall portion are spaced apart.
33. split tunnel as claimed in claim 2 wherein also comprises and the described first end wall portion second opposed end wall part, and the centre position place of at least a portion of described first flow path between this first end wall portion and second end wall portion.
34. split tunnel as claimed in claim 32 wherein also comprises the upstream side that is communicated with described dividing plate and second flow path in downstream, this second flow path is limited by the surface institute of described second end wall portion.
35. split tunnel as claimed in claim 3, wherein also comprise and the described first end wall portion second opposed end wall part, and at least a portion of described first flow path is positioned the place, centre position between this first end wall portion and second end wall portion.
36. split tunnel as claimed in claim 3, at least a portion of wherein said first flow path is limited by the surface of the axial setting of described dividing plate, and this surface is along at least a blood constituent of axial promotion of this split tunnel.
37. split tunnel as claimed in claim 3 wherein also comprises and the described first end wall portion second opposed end wall part, and wherein said first flow path is limited by the surface institute of this second end wall portion.
38. split tunnel as claimed in claim 35 wherein also comprises the upstream side that is communicated with described dividing plate and second flow path in downstream, this second flow path is limited by the surface institute of described second end wall portion.
39. split tunnel as claimed in claim 3, wherein said dividing plate extend to the inside radial position of described inner wall section.
40. split tunnel as claimed in claim 3, the surface of wherein said first flow path comprises described outer wall section.
41. split tunnel as claimed in claim 3, wherein also comprise the acquisition zone that is communicated with the described first mobile path flow body in described dividing plate downstream, this acquisition zone is at least in part by limiting with the isolated end wall branch of the described end wall portion of this split tunnel; And
First opening that is communicated with this acquisition zone and second opening are used to allow one or more fluid compositions to flow out from this acquisition zone.
42. split tunnel as claimed in claim 4, wherein also comprise the acquisition zone that is communicated with the described first mobile path flow body in described dividing plate downstream, this acquisition zone is at least in part by limiting with the isolated end wall branch of the described end wall portion of this split tunnel; And
First opening that is communicated with this acquisition zone and second opening are used to allow one or more fluid compositions to flow out from this acquisition zone.
43. split tunnel as claimed in claim 4 wherein also comprises first outlet pathway that is communicated with the split tunnel of described dividing plate upstream; Second outlet pathway that is communicated with split tunnel with this dividing plate downstream.
44. split tunnel as claimed in claim 43, wherein said first outlet pathway converges in a radially inner position of described inner wall section mutually with second outlet pathway.
45. split tunnel as claimed in claim 4 wherein also comprises and the described first end wall portion second opposed end wall part, and at least a portion of this first flow path is positioned the place, centre position between this first end wall portion and second end wall portion.
46. split tunnel as claimed in claim 4, at least a portion of wherein said first flow path is limited by the surface of the axial setting of described dividing plate, and this surface is along at least a blood constituent of axial promotion of this split tunnel.
47. split tunnel as claimed in claim 4, the surface of wherein said first flow path comprises described outer wall section.
48. split tunnel as claimed in claim 5, wherein said dividing plate extend to the inside radial position of described inner wall section.
49. split tunnel as claimed in claim 5, wherein said dividing plate meets at described outer wall section along sizable part of the axial length of this split tunnel.
50. split tunnel as claimed in claim 5, wherein said dividing plate extend between the radial position of described inside and outside wall part at least.
51. split tunnel as claimed in claim 5, wherein said outer wall section comprise a near part that is positioned at the described dividing plate, this part of this outer wall section is positioned at the outside of outer wall section of the split tunnel of this part upstream side diametrically.
52. split tunnel as claimed in claim 5, the surface of wherein said first flow path comprises described outer wall section.
53. split tunnel as claimed in claim 5 wherein also comprises with the first mobile path flow body in described dividing plate downstream being communicated with the 3rd outlet pathway, thereby can remove the fluid composition that is guided by this first flow path from this split tunnel.
54. split tunnel as claimed in claim 5 wherein also be included in a plurality of exit openings of this split tunnel in the downstream of described dividing plate, and in the upstream of this split tunnel, this split tunnel does not have exit opening.
55. split tunnel as claimed in claim 5, comprise also wherein and first of the described dividing plate downstream acquisition zone that the path flow body is communicated with that flows that this acquisition zone is at least in part by limiting with the axially spaced end wall branch of the described end wall portion of this split tunnel; And
First opening that is communicated with this acquisition zone and second opening are used to allow one or more fluid compositions to flow out from this acquisition zone.
56. split tunnel as claimed in claim 5, wherein also comprise and the described first end wall portion second opposed end wall part, and at least a portion of described first flow path is positioned the place, centre position between this first end wall portion and second end wall portion.
57. split tunnel as claimed in claim 5, at least a portion of wherein said first flow path limits the place by the surperficial institute of the axial setting of described dividing plate, and this surface is along at least a blood constituent of axial promotion of this split tunnel.
58. split tunnel as claimed in claim 5 wherein also comprise and the described first end wall portion second opposed end wall part, and the surface of wherein said first flow path is limited by this second end wall branch.
59. split tunnel as claimed in claim 6 wherein also comprise and the described first end wall portion second opposed end wall part, and the surface of described first flow path is limited by this second end wall branch.
60. split tunnel as claimed in claim 6, wherein said dividing plate extend to the inside radial position of described inner wall section.
61. split tunnel as claimed in claim 6, wherein said dividing plate meets at described outer wall section along sizable part of the axial length of this split tunnel.
62. split tunnel as claimed in claim 6, wherein said outer wall section comprise a near part that is positioned at the described dividing plate, this part of this outer wall section is positioned the outside of outer wall section of the split tunnel of this part upstream side diametrically.
63. split tunnel as claimed in claim 6 wherein also comprises first outlet pathway that is communicated with the split tunnel of described dividing plate upstream; With second outlet pathway that split tunnel with this dividing plate downstream is communicated with, this first outlet pathway converges in a radially inner position of described inner wall section mutually with second outlet pathway.
64. split tunnel as claimed in claim 6, comprise also wherein and first of the described dividing plate downstream acquisition zone that the path flow body is communicated with that flows that this acquisition zone is at least in part by limiting with the axially spaced end wall branch of the described end wall portion of this split tunnel; And
First opening that is communicated with this acquisition zone and second opening are used to allow one or more fluid compositions to flow out from this acquisition zone.
65. as the described split tunnel of claim 64, wherein said a plurality of exit openings comprise first opening and second opening that is communicated with described acquisition zone, are used to allow one or more fluid compositions to flow out from this acquisition zone.
66. split tunnel as claimed in claim 7 wherein also comprises first outlet pathway that is communicated with the split tunnel of described dividing plate upstream; Second outlet pathway that is communicated with split tunnel with described dividing plate downstream.
67. split tunnel as claimed in claim 7, wherein said dividing plate meets at described outer wall section along sizable part of the axial length of this split tunnel.
68. split tunnel as claimed in claim 7, wherein said outer wall section comprise a near part that is positioned at the described dividing plate, this part of this outer wall section is positioned the outside of outer wall section of the split tunnel of this part upstream side diametrically.
69. split tunnel as claimed in claim 7, the surface of wherein said first flow path comprises described outer wall section.
70. split tunnel as claimed in claim 7 wherein also comprises first outlet pathway that is communicated with the split tunnel of described dividing plate upstream; Second outlet pathway that is communicated with split tunnel with this dividing plate downstream.
71. a separation method may further comprise the steps:
First fluid is introduced in the centrifugal field, and wherein this first fluid comprises first composition and second composition that has different densities usually;
Permission forms interface at least this first composition and second composition between the several portions;
Remove second fluid from this interfacial side;
Remove the 3rd fluid from this interfacial opposite side;
At least a portion of this second fluid and first fluid or the 3rd combination of fluids are got up, and this fluid that combines is introduced in the described centrifugal field again;
From this centrifugal field, remove this second fluid or the 3rd fluid.
72. as the described method of claim 71, the position of wherein said interface in described centrifugal field forms, and this method is included in and introduces again before this fluid that combines, and changes the step of this interfacial described position.
73. as the described method of claim 71, wherein said first fluid comprises whole blood.
74. as the described method of claim 73, wherein said second fluid mainly comprises blood plasma.
75. as the described method of claim 73, wherein said the 3rd fluid mainly comprises red blood cell.
76. as the described method of claim 75, the hematocrit that wherein said second fluid that combines and first fluid or the 3rd fluid have is between about 20% and 40%.
77. as the described method of claim 74, wherein said blood plasma comprises blood platelet.
78. a separation method may further comprise the steps:
First fluid is introduced in the centrifugal field, and this first fluid comprises first composition and second composition that has different densities usually;
Permission forms interface at least this first composition and second composition between the several portions;
Reduce the power of this centrifugal field;
After reducing the power of this centrifugal field, from this centrifugal field, remove the first fluid composition.
79., wherein repeat the described step of removing more than once as the described method of claim 78.
80. as the described method of claim 78, the wherein said step of removing repeats at least twice.
81. as the described method of claim 78, wherein said first fluid comprises whole blood.
82. as the described method of claim 78, wherein said first fluid composition mainly comprises blood plasma.
83. as the described method of claim 82, wherein said blood plasma comprises blood platelet.
84. a separation method may further comprise the steps:
First fluid is introduced in the centrifugal field, and this first fluid comprises first composition and second composition that has different densities usually;
Permission forms interface at least this first composition and second composition between the several portions;
From this interfacial side, from this centrifugal field, sequentially and repeatedly remove fluid, and allow interface to form again.
85. as the described method of claim 84, wherein during removing, the power of described centrifugal field reduces, and again between the storage life, this power increases.
86. as the described method of claim 84, wherein said first fluid comprises whole blood.
87., wherein remove fluid at least twice from described centrifugal field as the described method of claim 84.
88. as the described method of claim 84, the wherein said step of removing comprises: make the hole of described fluid by being positioned at described interface one side, being communicated with described centrifugal field, and wherein this interface is moved with this hole close, be used to remove this fluid, and move away this hole, be used for forming again this interface.
89. as the described method of claim 84, the wherein said fluid of removing mainly comprises blood plasma and blood platelet.
90. a method that is used to gather blood constituent may further comprise the steps:
The blood source is connected in blood separating mechanism;
Blood is introduced by in the formed centrifugal field of this blood separating mechanism;
Permission forms interface between at least two kinds of blood constituents;
From this interfacial side, from this centrifugal field, remove the first fluid composition;
From this interfacial opposite side, from this centrifugal field, remove second fluid composition;
Store one of them kind of this first blood constituent and second blood constituent;
At least in part the another kind in this first blood constituent and second blood constituent is turned back to the blood source;
Extract other blood from this blood source;
Be connected with this blood source disconnection;
With this blood source disconnect be connected after, repeat the step of introducing and removing; And
First blood constituent that storage is removed from this centrifugal field and second blood constituent.
91. as the described method of claim 90, wherein said first fluid comprises at least three kinds of fluid compositions with different densities, and described storing step comprises each at least a portion that stores these three kinds of fluid compositions.
92. as the described method of claim 84, the wherein said step of removing comprises the several portions of removing described at least first composition and second composition from described centrifugal field.
93. as the described method of claim 84, wherein said first fluid comprises at least three kinds of fluid compositions with different densities, and may further comprise the steps: the several portions of from described centrifugal field, removing at least two kinds of compositions in these three kinds of fluid compositions.
94., wherein from described centrifugal field, remove the several portions of described three kinds of fluid compositions as the described method of claim 93.
95. as the described method of claim 78, the wherein said step of removing comprises remove the several portions of this first composition and second composition at least from described centrifugal field.
96. as the described method of claim 78, wherein said first fluid comprises at least three kinds of fluid compositions, and may further comprise the steps: the several portions of from described centrifugal field, removing at least two kinds of compositions in these three kinds of fluid compositions.
97., wherein from described centrifugal field, remove the several portions of described three kinds of fluid compositions as the described method of claim 96.
98. as the described method of claim 71, the wherein said step of removing comprises the several portions of removing described first composition and second composition from described centrifugal field.
99. as the described method of claim 71, wherein said first fluid comprises at least three kinds of fluid compositions, and may further comprise the steps: at least two kinds several portions from described centrifugal field, removing these three kinds of fluid compositions.
100. as the described method of claim 99, the several portions of wherein said three kinds of fluid compositions is removed from described centrifugal field.
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CN101743067B (en) * 2007-06-05 2011-08-17 泰尔茂株式会社 Insert and centrifuge comprising an insert
CN101450336B (en) * 2007-12-06 2012-02-08 财团法人工业技术研究院 Fluid separation method and device
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CN103191480A (en) * 2012-01-09 2013-07-10 金卫医疗科技(上海)有限公司 Method for increasing blood plasma extraction purity during continuous centrifugal blood separation
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US6284142B1 (en) * 1999-09-03 2001-09-04 Baxter International Inc. Sensing systems and methods for differentiating between different cellular blood species during extracorporeal blood separation or processing

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CN101790422B (en) * 2007-06-05 2011-08-17 泰尔茂株式会社 Insert and centrifuge comprising an insert
CN101743067B (en) * 2007-06-05 2011-08-17 泰尔茂株式会社 Insert and centrifuge comprising an insert
CN101450336B (en) * 2007-12-06 2012-02-08 财团法人工业技术研究院 Fluid separation method and device
CN102378911A (en) * 2009-02-04 2012-03-14 莱泽尔诊断公司 Apparatus and methods for separating and analyzing components in fluids
CN103191480A (en) * 2012-01-09 2013-07-10 金卫医疗科技(上海)有限公司 Method for increasing blood plasma extraction purity during continuous centrifugal blood separation
CN103191480B (en) * 2012-01-09 2015-05-20 金卫医疗科技(上海)有限公司 Method for increasing blood plasma extraction purity during continuous centrifugal blood separation
CN104185483A (en) * 2012-03-27 2014-12-03 泰尔茂株式会社 Blood component separation device
CN109922842A (en) * 2016-09-16 2019-06-21 汾沃有限公司 Using the blood separation system and method for being centrifuged and rotating membrane separation technique
CN110621410A (en) * 2017-05-12 2019-12-27 科学产品有限公司 Compact countercurrent centrifuge system
CN110621410B (en) * 2017-05-12 2022-08-02 科学产品有限公司 Compact countercurrent centrifuge system
CN112619905A (en) * 2021-01-12 2021-04-09 陈恢松 High-low speed matched operation biological centrifugal equipment based on speed-up control
CN112619905B (en) * 2021-01-12 2022-12-23 山东富优生命科学有限公司 High-low speed running-matched biological centrifugal equipment based on speed-up control

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