CN1900699A - Method for detecting cotton leaf chlorophyll content based on image technique - Google Patents
Method for detecting cotton leaf chlorophyll content based on image technique Download PDFInfo
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- CN1900699A CN1900699A CNA2005100854686A CN200510085468A CN1900699A CN 1900699 A CN1900699 A CN 1900699A CN A2005100854686 A CNA2005100854686 A CN A2005100854686A CN 200510085468 A CN200510085468 A CN 200510085468A CN 1900699 A CN1900699 A CN 1900699A
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Abstract
Belonging to method for testing physiologic indexes of crop in agriculture, the disclosed method is based color change of blade caused by change of chlorophyll content in cotton blade. Taking picture of cotton blade, the method quantizes color change by using computer image technique, and establishes corresponding functional relation between quantized color change and chlorophyll content in cotton blade. Thus, if knowing color value of blade, the method can calculate chlorophyll content in blade through the said functional relation so as to realize purpose of mensurating chlorophyll content in cotton blade by using image technique. Comparing with traditional method, the disclosed method possesses advantages of shorter time consumed, less steps, and simpler operations etc. The invention is applicable to determining growth condition of crop, and guiding fertilization and irrigation in production of crop.
Description
1, technical field
" based on the cotton leaf chlorophyll measuring method of image technology " belongs to the plant physiology indication test method in the agricultural.
2, background technology
Carrying out crop leaf chlorophyll measuring method commonly used in the agricultural at present is spectrophotometer method, be about to crop leaf and fetch the laboratory, blade weighed shred, use the acetone extraction leaf chlorophyll, use the optical density of spectrophotometric determination extract then at 645nm and 663nm, represent with OD645 and OD663 respectively, be converted into chlorophyll content then as follows.
Chlorophyll a content=12.7 * OD663-2.59 * OD645
Chlorophyll b content=22.9 * OD645-4.67 * OD663
Chlorophyll a+b content=20.3 * OD645+8.04 * OD663
Also having a kind of method is to measure the value that blade directly reads chlorophyll content in leaf blades with chlorophyll meter.With maximum be the SPAD-502 that Japanese Minolta company produces, it is according to leaf chlorophyll the characteristics that absorb paddy and reflection peak to be arranged in specific wavelength position, visible region, the rayed of launching a branch of known strength by the instrument of making detects tested blade specific wavelength absorptivity and reflectivity and calculates chlorophyll content in leaf blades at the blade position that need are measured.
3, summary of the invention
" based on the cotton leaf chlorophyll measuring method of image technology " that I propose, be to change according to the cotton leaf chlorophyll content to cause that leaf color changes, by taking the cotton leaf image, with the computing machine image technology this change in color is quantized, and set up corresponding funtcional relationship with chlorophyll content in leaf blades, as long as promptly known the color value of blade, just can calculate chlorophyll content in leaf blades by this funtcional relationship, realize measuring the purpose of cotton leaf chlorophyll content with image technology.
4, embodiment
This method comprises three parts:
First is that the blade image obtains.Under fine indoor natural light face of blade is made progress, open and flat placing on the black background can be obtained the leaf digital image by scanner or digital camera, when obtaining the blade image with digital camera, requires camera to take perpendicular to blade, and shadow-free on the blade.
Second portion is the blade image processing.With the leaf digital that obtains image image processing software, as Photoshop6.0 etc., R, G, the B value of extraction whole blade, by formula carry out following calculating again and obtain r, g, b value and S value respectively:
r＝R/(R+G+B)
g＝G/(R+G+B)
b＝B/(R+G+B)
Third part is with the leaf color value of obtaining, and brings the leaf color value of foundation and the functional expression between chlorophyll content in leaf blades into, calculates chlorophyll content in leaf blades.Cotton leaf color value of setting up by my research and the functional expression between chlorophyll content have:
(1) b and chlorophyll b: y=-10.8+63.2x
(2) b and chlorophyll a+b:y=-34.6+183.57x
(3) S and chlorophyll a+b:y=27.09-60.22x
X is the leaf color value in the above-mentioned functional expression, and y is the chlorophyll content in leaf blades that determines.
This assay method is compared with spectrophotometer method, it is advantageous that fast, easy, saves time, and is laborsaving, saves cost, simple to operate, and weak point is to measure precision not as the spectrophotometer method height, but also can satisfy the needs of guiding agricultural production fully.With compare with the SPAD assay method, because of the mensuration of SPAD is a contact, the numerical value that reads only is the chlorophyll content value at place, instrument probe contact, can not represent the chlorophyll content level of whole blade, need carry out big quantitative determination if will obtain the chlorophyll content of whole blade, and the method that I propose, the image that a blade is only needed to take an intact leaf obtains the local chlorophyll content of blade, also can obtain the whole blade chlorophyll content.The outstanding advantage that shows is that cost is low, flexible operation, efficient height, precision height.If the method is carried out functionalization, can produce portable cotton leaf chlorophyl-meter.
Claims (2)
- " based on the green cellulose content assay method of cotton leaf of image technology " is to obtain the cotton leaf digital image by sweeping instrument or digital camera, extract the leaf color value by visual Plank technology, calculate a kind of new method of determined chlorophyll content according to the funtcional relationship of leaf color and chlorophyll content.For " based on the cotton chlorophyll content in leaf blades assay method of image technology ", the patent right that requires to obtain is:1, is used to measure the method for cotton leaf chlorophyll content with the digital image technology.
- 2, the functional expression between cotton leaf color value and chlorophyll content in leaf blades comprises:(1) the content functional expression of b and chlorophyll b: y=-10.8+63.2x(2) the content functional expression of b and chlorophyll a+b: y=-34.6+183.57x(3) the content functional expression of S and chlorophyll a+b: y=27.09-60.22x
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| CNA2005100854686A CN1900699A (en) | 2005-07-21 | 2005-07-21 | Method for detecting cotton leaf chlorophyll content based on image technique |
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| CNA2005100854686A CN1900699A (en) | 2005-07-21 | 2005-07-21 | Method for detecting cotton leaf chlorophyll content based on image technique |
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| CN1900699A true CN1900699A (en) | 2007-01-24 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101556244B (en) * | 2009-05-22 | 2011-07-27 | 天津大学 | Measurement method for chlorophyll content in cucumber leaves based on RGB chlorophyll meter |
| CN102519886A (en) * | 2011-12-19 | 2012-06-27 | 浙江大学 | Method for detecting contents of chlorophyll a and carotinoid in crop laminas |
| CN104089895A (en) * | 2014-07-24 | 2014-10-08 | 四川农业大学 | Photosynthetic pigment measurement method and photosynthetic pigment measurement system for soybean leaves |
| CN105890764A (en) * | 2016-03-31 | 2016-08-24 | 杭州市农业科学研究院 | Plant flower color shade distinguishing method based on computer image processing |
| CN106596863A (en) * | 2016-12-22 | 2017-04-26 | 邬东强 | System and method for acquiring plant information based on camera of mobile phone |
| CN108680513A (en) * | 2018-06-12 | 2018-10-19 | 山东省林业科学研究院 | Plant chlorophyll assay method and extraction element |
| CN110596098A (en) * | 2019-08-26 | 2019-12-20 | 江苏大学 | Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves |
-
2005
- 2005-07-21 CN CNA2005100854686A patent/CN1900699A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101556244B (en) * | 2009-05-22 | 2011-07-27 | 天津大学 | Measurement method for chlorophyll content in cucumber leaves based on RGB chlorophyll meter |
| CN102519886A (en) * | 2011-12-19 | 2012-06-27 | 浙江大学 | Method for detecting contents of chlorophyll a and carotinoid in crop laminas |
| CN104089895A (en) * | 2014-07-24 | 2014-10-08 | 四川农业大学 | Photosynthetic pigment measurement method and photosynthetic pigment measurement system for soybean leaves |
| CN104089895B (en) * | 2014-07-24 | 2016-08-24 | 四川农业大学 | The photosynthetic pigments assay method of a kind of soybean leaves and system |
| CN105890764A (en) * | 2016-03-31 | 2016-08-24 | 杭州市农业科学研究院 | Plant flower color shade distinguishing method based on computer image processing |
| CN105890764B (en) * | 2016-03-31 | 2018-01-23 | 杭州市农业科学研究院 | The method of the resolution flower color depth based on Computer Image Processing |
| CN106596863A (en) * | 2016-12-22 | 2017-04-26 | 邬东强 | System and method for acquiring plant information based on camera of mobile phone |
| CN108680513A (en) * | 2018-06-12 | 2018-10-19 | 山东省林业科学研究院 | Plant chlorophyll assay method and extraction element |
| CN108680513B (en) * | 2018-06-12 | 2020-08-25 | 山东省林业科学研究院 | Plant chlorophyll determination method and extraction device |
| CN110596098A (en) * | 2019-08-26 | 2019-12-20 | 江苏大学 | Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves |
| CN110596098B (en) * | 2019-08-26 | 2022-05-20 | 江苏大学 | Rapid detection system and detection method for ratio distribution of chlorophyll and lutein in cucumber leaves |
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Open date: 20070124 |