CN1876643A - Method for extracting apple polyphenol from concentrated apple juice production - Google Patents
Method for extracting apple polyphenol from concentrated apple juice production Download PDFInfo
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- CN1876643A CN1876643A CN 200510017666 CN200510017666A CN1876643A CN 1876643 A CN1876643 A CN 1876643A CN 200510017666 CN200510017666 CN 200510017666 CN 200510017666 A CN200510017666 A CN 200510017666A CN 1876643 A CN1876643 A CN 1876643A
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- polyphenol
- elutriant
- organic solvent
- eluent
- apple
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Abstract
The invention relates the preparing method of extracting apple polyphenol, belonging to extracting polyphenol technology field. The invention extracts polyphenol from decolouring column eluent. When the eluent is strong aqueous alkali, eluent is neutralized with acid, evaporated and concentrated, getting polyphenol. When the eluent is organic solvent or organic solvent water solution, the eluent is evaporated and concentrated, getting polyphenol. The invention has the advantages of improving apple availability ratio, reducing discharge of resin regeneration eluent, reducing pollution, modifying ecologic environment and good economic benefit and social benefit.
Description
Technical field
The invention belongs to polyphenol extractive technique field, particularly a kind of method of in the fruit juice production technique, taking decolorizing column elutriant separation and Extraction apple polyphenol.
Background technology
The concentrated Succus Mali pumilae production process is as follows: (1) raw material is really purchased: before producing the arriving in season every year apple purchase zone is generally investigated, to determine the purchase zone of safety.The apple of acceptance(check) is deposited in the fruit storehouse.(2) broken squeezing: choose apple after qualified and enter that grinding machine is broken to be squeezed.(3) pre-crust kills: fruit juice groove fruit juice is squeezed into turbid juice jar through the fruit juice pump and is carried out pre-sterilizing through interchanger again.(4) enzymolysis: the fruit juice after pre-crust kills enters enzymatic vessel through the plate-type heat exchanger cooling and carries out enzymolysis.(5) membrane filtration: the abundant fruit juice of enzymolysis, be pumped to respectively membrane filter appts filter clear juice.(6) decolouring: clear juice pumps into decolorizing column by clear juice jar and handles.(7) concentrate: the fruit juice after the decolouring pumps into vaporizer and concentrates.(8) crust kills: the fruit juice after concentrating carries out final pasteurising.(9) can: the fruit juice after crust kills cools off through interchanger, carries out sterile filling by filling machine.(10) packing: finished product be packaged as sterile bag, steel drum, plastic lining bag, liquid bag.(11) store: the finished product concentrated apple clear juice leaves in the freezer in batches.
In this production process, behind the decolorizing column operation certain hour, lose decolorization, need the resin in the decolorizing column is handled so that resin recovers decoloring ability.When resin regeneration, the regenerator wash-out is adsorbed on the coloring matter on the resin, and makes resin recover decoloring ability.These coloring matters that are adsorbed on the resin are discharged after by wash-out, wherein contain polyphenols, and that polyphenols has is anti-oxidant, remove free radical, anti-dental caries, antianaphylaxis, multiple biological activity such as hypotensive, and the recycle value of elutriant is very big.And directly discharging also can bring a series of environmental issue.
Summary of the invention
The object of the invention is to provide a kind of method of extracting apple polyphenol from the concentrated Succus Mali pumilae production process.
For reaching above-mentioned purpose, the present invention adopts following technical scheme: extract the method for apple polyphenol from the concentrated Succus Mali pumilae production process, extract polyphenol from the decolorizing column elutriant.
Eluent is a strong alkali aqueous solution, and elutriant gets polyphenol with acid neutralization back evaporation concentration.
Aqueous sodium hydroxide solution with the weight percent concentration 0.5-15% of 1-3 times of volume of need regeneration polymeric adsorbent volume carries out regenerative elution at 15-75 ℃, and elutriant is neutralized to PH5.5-8.5 with the 5---10% hydrochloric acid soln, and evaporation concentration, drying must polyphenol.
Eluent is organic solvent or aqueous solutions of organic solvent, and the elutriant evaporation concentration gets polyphenol.
Aqueous solutions of organic solvent with the weight percent concentration 10-90% of 1-3 times of volume of need regeneration polymeric adsorbent volume carries out regenerative elution, elutriant evaporation concentration, drying, polyphenol.
Organic solvent is an ethanol.
Organic solvent is an acetone.
Organic solvent is a methyl alcohol.
The present invention carries out separation and Extraction in conjunction with the production technique of concentrated Succus Mali pumilae to wherein resin column (decolorizing column) regenerative elution liquid, obtain apple polyphenol, can make full use of resin regenerative elution liquid on the existing fruit juice concentrate production line, extract a large amount of fine apple polyphenol.Enriched the degree of depth of apple processing on the one hand, improved the comprehensive utilization ratio of apple, can reduce the discharging of resin regeneration elutriant on the other hand again, reduced pollution, improved the ecological environment, very significantly economic benefit and social benefit have been arranged environment.
The present invention can comprise organic solvents such as alkaline solution and methyl alcohol, ethanol, acetone, propyl alcohol, Virahol, hexanaphthene with eluent, and total phenol content is 20-98% in the polyphenol extract, wherein mainly comprises chlorogenic acid, Flavonol etc.
Embodiment
Embodiment 1, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, 10% sodium hydroxide solution regeneration with 1 times of resin volume, 70 ℃ of temperature, elutriant is neutralized to pH6.5 with hydrochloric acid, then the solution after the neutralization is concentrated with rotatory evaporator, is concentrated into 1/3 volume, concentrated solution is put into 50 ℃ of vacuum-dryings of vacuum drying oven, can obtain the apple polyphenol material.
Embodiment 2, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, 0.5% sodium hydroxide solution regeneration with 1.5 times of resin volumes, 30 ℃ of temperature, elutriant is neutralized to pH7.5 with hydrochloric acid, then the solution after the neutralization is concentrated with rotatory evaporator, concentrated solution is put into 40 ℃ of vacuum-dryings of vacuum drying oven, can obtain polyphenol substance.
Embodiment 3, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, 5% sodium hydroxide solution regeneration with 3 times of resin volumes, 55 ℃ of temperature, elutriant is neutralized to pH7.0 with hydrochloric acid, then the solution after the neutralization is concentrated with rotatory evaporator, concentrated solution is put into 70 ℃ of vacuum-dryings of vacuum drying oven, can obtain polyphenol substance.
Embodiment 4, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, with 4 times of volume of ethanol aqueous solution regeneration, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, and the 40 ℃ of vacuum-dryings of elutriant after concentrating get the apple polyphenol extract.
Embodiment 5, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, regenerate with 3 volume of ethanol solution, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, elutriant spraying drying after concentrating gets the apple polyphenol extract.
Embodiment 6, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, regenerate with 1 volume of ethanol solution, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, elutriant vacuum-drying after concentrating gets the apple polyphenol extract.
Embodiment 7, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, methanol solution regeneration with 4 times of volumes, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, and the 40 ℃ of vacuum-dryings of elutriant after concentrating get the apple polyphenol extract.
Embodiment 8, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, methanol solution regeneration with 3 times of volumes, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, elutriant spraying drying after concentrating gets the apple polyphenol extract.
Embodiment 9, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, methanol solution regeneration with 1 times of volume, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, elutriant vacuum-drying after concentrating gets the apple polyphenol extract.
Embodiment 10, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000kg regenerative elution liquid (regenerating) with sodium hydroxide solution, normal temperature is neutralized to pH6.8 with 5% hydrochloric acid down, use the vacuum-evaporator vacuum concentration then, elutriant vacuum-drying after concentrating gets the apple polyphenol extract.
Embodiment 11, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000kg regenerative elution liquid (regenerating) with sodium hydroxide solution, normal temperature is neutralized to pH6.8 with 5% hydrochloric acid down, use the vacuum-evaporator vacuum concentration then, elutriant spraying drying after concentrating gets the apple polyphenol extract.
Embodiment 12, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000kg regenerative elution liquid (regenerating) with sodium hydroxide solution, normal temperature is neutralized to pH7.2 with 10% hydrochloric acid down, use the vacuum-evaporator vacuum concentration then, elutriant vacuum-drying after concentrating gets the apple polyphenol extract.
Embodiment 13, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 2000ml and need the regenerated polymeric adsorbent, at ambient temperature, acetone soln with 4 volumes is regenerated, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, and the 40 ℃ of vacuum-dryings of elutriant after concentrating get the apple polyphenol extract.
Embodiment 14, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 1000ml and need the regenerated polymeric adsorbent, at ambient temperature, acetone soln with 3 volumes is regenerated, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, elutriant vacuum spray drying after concentrating gets the apple polyphenol extract.
Embodiment 15, from the concentrated Succus Mali pumilae production process, extract the method for apple polyphenol, from fruit juice concentrate production, get 2000ml and need the regenerated polymeric adsorbent, at ambient temperature, acetone soln with 1 volume is regenerated, collect elutriant, elutriant reclaims eluent simultaneously with the vacuum-evaporator vacuum concentration, elutriant vacuum-drying after concentrating gets the apple polyphenol extract.
Claims (8)
1, from the concentrated Succus Mali pumilae production process, extracts the method for apple polyphenol, it is characterized in that, extract polyphenol from the decolorizing column elutriant.
2, the method for claim 1 is characterized in that, eluent is a strong alkali aqueous solution, and elutriant gets polyphenol with acid neutralization back evaporation concentration.
3, method as claimed in claim 2, it is characterized in that, aqueous sodium hydroxide solution with the weight percent concentration 0.5-15% of 1-3 times of volume of need regeneration polymeric adsorbent volume carries out regenerative elution at 15-75 ℃, elutriant is neutralized to PH5.5-8.5 with the 5-10% hydrochloric acid soln, evaporation concentration, drying get polyphenol.
4, the method for claim 1 is characterized in that, eluent is organic solvent or aqueous solutions of organic solvent, and the elutriant evaporation concentration gets polyphenol.
5, method as claimed in claim 4 is characterized in that, carries out regenerative elution with the aqueous solutions of organic solvent of the weight percent concentration 10-90% that needs 1-3 times of volume of regeneration polymeric adsorbent volume, elutriant evaporation concentration, drying, polyphenol.
As claim 4 or 5 described methods, it is characterized in that 6, organic solvent is an ethanol.
As claim 4 or 5 described methods, it is characterized in that 7, organic solvent is an acetone.
As claim 4 or 5 described methods, it is characterized in that 8, organic solvent is a methyl alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100176669A CN1876643B (en) | 2005-06-09 | 2005-06-09 | Method for extracting apple polyphenol from concentrated apple juice production |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100176669A CN1876643B (en) | 2005-06-09 | 2005-06-09 | Method for extracting apple polyphenol from concentrated apple juice production |
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| Publication Number | Publication Date |
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| CN1876643A true CN1876643A (en) | 2006-12-13 |
| CN1876643B CN1876643B (en) | 2010-09-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2005100176669A Expired - Fee Related CN1876643B (en) | 2005-06-09 | 2005-06-09 | Method for extracting apple polyphenol from concentrated apple juice production |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829210A (en) * | 2010-05-21 | 2010-09-15 | 西安应化生物技术有限公司 | Method for preparing apple polyphenol |
| CN102125265A (en) * | 2010-11-11 | 2011-07-20 | 陕西科技大学 | Recovery method of polyphenol during processing apple concentrated juice |
| CN103340412A (en) * | 2013-07-16 | 2013-10-09 | 天水长城果汁集团有限公司 | Method for preparing free water with natural polyphenols and essence |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1190152C (en) * | 2003-04-16 | 2005-02-23 | 连云港市东海果汁有限公司 | Apple juice and its preparation method |
-
2005
- 2005-06-09 CN CN2005100176669A patent/CN1876643B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829210A (en) * | 2010-05-21 | 2010-09-15 | 西安应化生物技术有限公司 | Method for preparing apple polyphenol |
| CN102125265A (en) * | 2010-11-11 | 2011-07-20 | 陕西科技大学 | Recovery method of polyphenol during processing apple concentrated juice |
| CN102125265B (en) * | 2010-11-11 | 2013-02-13 | 陕西科技大学 | Recovery method of polyphenol during processing apple concentrated juice |
| CN103340412A (en) * | 2013-07-16 | 2013-10-09 | 天水长城果汁集团有限公司 | Method for preparing free water with natural polyphenols and essence |
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| Publication number | Publication date |
|---|---|
| CN1876643B (en) | 2010-09-08 |
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