CN1875717A - Biological mixed pesticide for insect control in green vegetables field - Google Patents
Biological mixed pesticide for insect control in green vegetables field Download PDFInfo
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- CN1875717A CN1875717A CN 200610010264 CN200610010264A CN1875717A CN 1875717 A CN1875717 A CN 1875717A CN 200610010264 CN200610010264 CN 200610010264 CN 200610010264 A CN200610010264 A CN 200610010264A CN 1875717 A CN1875717 A CN 1875717A
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Abstract
The invention relates to a pesticide for vegetable field, which is the composite agent of Bacillus thuringiensis Bt and success, each 100ml of the agent contain Bacillus thuringiensis Bt spores of the following number: 2X10<9> to 7X10<9>, and success 0.01-0.04ml. The agent has quick effect.
Description
Technical field
The present invention relates to a kind of insecticide, be specifically related to a kind of insecticide that is used for green food vegetable Tanaka pest control.
Background technology
Pest are annual has all caused tremendous loss for plant husbandry.Though chemical control obtains remarkable effect, a series of problems such as environmental pollution, pesticide resistance have also been brought simultaneously.Since the seventies in 20th century, biological control has been subjected to the attention of national governments.The 21st resolution of 1992 " world environments and development conference " proposes " sale and the use of inner control chemical pesticide in the world by 2000, the output of biopesticide reaches 60% ".Since the nineties, the sales volume of whole world chemical pesticide constantly descends, and what replace it is microbial pesticide.The Chinese government also attaches great importance to soil and water resource environment protection, and " development of pollution-free food and biopesticide " listed in Agenda 21.Microbial pesticide as the biological control important content, have the advantages that people, animal and pest natural enemy seldom or fully there are not toxic action, free from environmental pollution, keep ecological balance, keep the sustainable development of agricultural, forestry, adapt to the demand of people to nuisanceless agricultural byproducts, pollution-free food.
After the China joined WTO, in international agriculture and food trade, will be in the face of the severe challenge of international green barrier, i.e. the Ke Ke trace standard of pesticide.This measure causes China to wait the export of farm produce of country limited to American-European-Japanese.The optimal path that the solution toxicity of pesticide exceeds standard is exactly to replace traditional high poison, the chemical pesticide of high residue with biopesticide.Existing bacillus thuringiensis biopesticide ubiquity the desinsection slow phenomenon that takes effect, and generally is that the 4th talent's beginning effect is apparent in view after the medication, but also exists bacillus thuringiensis Bt unitary agent to the not good problem of noctuidae pests control.
Summary of the invention
In order to solve the deficiency that prior art exists, the invention provides a kind of biological mixed pesticide of green food vegetable Tanaka pest control.
Insecticidal compound agent of the present invention is bacillus thuringiensis Bt and success, and its Chinese name is called auspiciousness composite dose of dish.
Success is that the farming of LG-DOW benefit is so kind as to give, and mainly plays auxiliary insecticidal action, is stomach poison, and Success can sustained activation target insect acetylcholine nicotine receptor, but its binding site is different from nicotine and Imidacloprid.Pleocidin also can influence the GABA acceptor, has come insecticidal action.Its main component of Success is spinosynA:C
41H
65NO
16And spinosynB:C
42H
67NO
16, molecular structural formula is:
The insecticidal spectrum of insecticide of the present invention is tomato moth, diamond-back moth, beet webworm etc.
Existing bacillus thuringiensis biopesticide ubiquity the desinsection slow phenomenon that takes effect, and generally is that the 4th day beginning effect is apparent in view after the medication, and uses this prescription just apparent in view at the insecticidal effect on the same day or second day; Simultaneously, insecticidal effect is also obvious than original bacillus thuringiensis biopesticide insecticidal effect.From whole insecticidal effect, the insecticidal effect of insecticidal formulation of the present invention obviously is better than like product in the past.
The biological insecticides of the present invention's development are high activity bacillus thuringiensis bacterial strain and the success compound formulation that comes from microorganism.Filtered out the high-activity biological insecticide success good by bioassay method with to bacillus thuringiensis-Bt20 vitality test with the bacillus thuringiensis compatibility, and with its another important insect disinfestation component as serial bacillus thuringiensis composite insecticide, by indoor measurement, potted plant and sub-district, field control test, obtained the satisfied control efficiency of people in addition, not only can improve the insecticidal effect of bacillus thuringiensis, and can improve the quick-acting of bacillus thuringiensis desinsection, make the target pest lethal time improve more than 24 hours.This kind biologic product prescription still belongs to the first time at home.Biopesticide of the present invention is the biologic product that meets AA level pollution-free food and international organic farming production standard.The invention solves the slow problem that takes effect when in the past using biopesticide; Replacing the chemical pesticide of traditional high poison, high residue to solve toxicity of pesticide with biopesticide exceeds standard, has following advantage:
1, the residue of pesticide in the reduction vegetables;
2, quicken the responding time of biopesticide;
3, reduce the toxic action of agricultural chemicals to people, animal and pest natural enemy;
4, reduce chemical pesticide to the pollution of environment, keep ecological balance, keep the sustainable development of agricultural, forestry, adapt to the demand of people to nuisanceless agricultural byproducts, pollution-free food.
Embodiment
Embodiment one: the insecticidal compound agent of present embodiment is bacillus thuringiensis Bt and success (it is auspiciousness that its Chinese name is called dish) composite a dose, contains bacillus thuringiensis Bt gemma number among composite dose of wherein every 100ml: 2 * 10
9～7 * 10
9Individual, dish is auspiciousness: 0.01～004ml.
Embodiment two: the bacillus thuringiensis Bt of present embodiment adopts bacillus thuringiensis (Bacillus Thuringiensis) Bt20, the consisting of of its insecticidal compound agent: contain Tween-60 or Tween-80 (the 60, the 80th, the trade name of tween) in every 100ml finished product: 2 grams, citric acid: 1～3 gram, methylcellulose: 1～4 gram, diatomite: 0.2～0.4 gram, Sodium Benzoate: 0.5～2 gram, bacillus thuringiensis (BacillusThuringiensis) Bt20 gemma number: 2 * 10
9～7 * 10
9Individual, success:0.01～0.04ml.
Bacterial strain bacillus thuringiensis (Bacillus thuringiensis) Bt20 used in the present embodiment is called for short Bt20, in on May 11st, 2006 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC NO.1710.It plays insecticidal action in insecticide, that is: bacillus thuringiensis can form the protein crystal of a kind of being called " endotoxin ", this endotoxin is to the people, raise nontoxic, behind the insect's food-taking bacillus thuringiensis, the endotoxin crystal dissolves in the enteron aisle of insect, through the protease elaboration in the insect gut, be degraded into virose proteotoxin, with the lip-deep receptors bind of intestinal cell, insert in the film, form hole or ion channel and cause the complete destruction of film, cause the ion seepage, water enters cell thereupon, and cell disintegrates because of expansion, death, final insect death.
In the present embodiment, the mass concentration of described success is 2.5%.
The gemma counting carries out as follows in the present embodiment: in desinfection chamber, add the 2ml sterile water in cultured inclined-plane, scrape lawn, blow and beat repeatedly with pipettor and make its abundant mixing in sterile water, bacterium liquid is moved in the sterile vials; Press gradient dilution 10 then
4～10
5Doubly; On blood counting chamber, drip several bacterium liquid that diluted, build cover glass, look into number and write down average every lattice gemma number, calculate every milliliter of bacterium liquid and contain the gemma number, obtain original bacteria liquid concentration at microscopically.
The Bt20 of present embodiment can separate acquisition from soil, concrete preparation method is:
(1) main material:
The soil of sterile water, polymyxin B, beef extract, peptone, agar, cause of disease to be separated, microscope.
(2) operating procedure:
I. 1 gram soil is suspended in 10 ml sterile waters, strong vibration was left standstill 5 minutes then.
Ii. with the sterilization suction pipe upper strata is suspended and change in the sterilization test tube, carry out 10 times of serial dilutions with sterile water, each dilution suspension put into for 1/10 milliliter sterilize and the agar medium (pH=8.5 of good beef extract-peptone, the polymyxin B that contains 5ppm), shakeout with sterilization triangle glass spatula, 30 ℃, 2 days, with regard to the separable Bt20 that arrives.
It should be noted that:
The cultivation proterties of i:Bt20: see Table 1.
The cultivation proterties on the beef extract-peptone agar slant of table 1 Bt20
Ii:Bt 20 has 4 esterase district bands, and strong band is at Ef value and 43 places, and is identical with Kustak subspecies esterase-type, sees Table 2.
Table 2 Bt20 bacterial strain district's band number and Ef value
| Bacterial strain the kind of strains | District band number the number of esterase bands | The Ef value Ef of different esterase band of each district's band |
| Bt20 | 4 | 11，28，43，50 |
Embodiment three: the consisting of of the insecticidal compound agent of present embodiment: contain Tween-60 or Tween-80 in every 100ml finished product: 2 grams, citric acid: 1 gram, methylcellulose: 1 gram, diatomite: 0.2 gram, Sodium Benzoate: 0.5 gram, bacillus thuringiensis (Bacillus Thuringiensis) Bt20 gemma number: 2 * 10
9Individual, mass concentration is 2.5% success:0.01ml.
Embodiment four: the consisting of of the insecticidal compound agent of present embodiment: contain Tween-60 or Tween-80 in every 100ml finished product: 2 grams, citric acid: 3 grams, methylcellulose: 4 grams, diatomite: 0.4 gram, Sodium Benzoate: 2 grams, bacillus thuringiensis (Bacillus Thuringiensis) Bt20 gemma number: 7 * 10
9Individual, mass concentration is 2.5% success:004ml.
Embodiment five: the consisting of of the insecticidal compound agent of present embodiment: contain Tween-60 or Tween-80 in every 100ml finished product: 2 grams, citric acid: 1.5 grams, methylcellulose: 2.6 grams, diatomite: 0.3 gram, Sodium Benzoate: 1 gram, bacillus thuringiensis (Bacillus Thuringiensis) Bt20 gemma number: 4 * 10
9Individual, mass concentration is 2.5% success:0.02ml.The test of pesticide effectiveness of above-mentioned insecticide is as follows:
1, the screening of high activity bacillus thuringiensis strains:
Screening technique: soak the bacillus thuringiensis,Bt zymotic fluid with cabbage leaves, the diamond-back moth of feeding after air-dry, the death condition of observing diamond-back moth in 24,72,120,168 hours is calculated lethality and corrected mortality.Control treatment is to soak cabbage leaves with clear water.
Through Preliminary screening, the insecticidal activity that obtains Bt20 is the strongest in selected bacterial strain, and its corrected mortality is 85.40%, and concrete outcome sees Table 3.
Table 3 bacillus thuringiensis strains is to the indoor bioassay of diamond-back moth
| Bacterial strain | Handle | 24h | 72h | 120h | 168h | ||||
| Lethality (%) | Corrected mortality (%) | Lethality (%) | Corrected mortality (%) | Lethality (%) | Corrected mortality (%) | Lethality (%) | Corrected mortality (%) | ||
| Bt20 | On average | 62.22 | 58.50 | 84.41 | 82.90 | 86.67 | 85.40 | 86.67 | 85.40 |
| Contrast | On average | 2.22 | - | 4.44 | - | 8.89 | - | 8.89 | - |
2, bacillus thuringiensis,Bt and Success use with the preventive effect of main vegetable-crop pest-insect are measured:
The Bt20 bacterial classification is contained gemma 1 * 10 by every milliliter
8Bacterium liquid, Success with 6000 times, 9000 times liquid of dilution uses with respectively, diamond-back moth is carried out indoor, outdoor insecticidal activity assay, show that by 2 years result of the tests Success has obvious synergistic effect to bacillus thuringiensis,Bt, 48～72h reaches more than 90% the indoor preventive effect of diamond-back moth behind the medicine, and sub-district, field control efficiency is stabilized in more than 80%～85%.Indoor control effect to cabbage caterpillar, tomato moth is 60%～70% (table 4～6).
Table 4 Success uses the indoor measurement to the diamond-back moth preventive effect with
| Handle | Working concentration | Repeat | Lethality 24h (%) | Corrected mortality 24h (%) | Lethality 48h (%) | Corrected mortality 48h (%) | 72h lethality (%) | Corrected mortality 72h (%) |
| SUCCESS | 9000 times of liquid | 1 | 33.33 | 30.21 | 46.67 | 42.86 | 53.33 | 48.77 |
| 2 | 40.00 | 37.19 | 53.33 | 49.99 | 60.00 | 56.09 | ||
| 3 | 33.33 | 30.21 | 33.33 | 28.56 | 46.67 | 41.46 | ||
| On average | 35.55 | 32.54 | 44.44 | 40.47 | 53.33 | 48.77 |
| 6000 times of liquid | 1 | 40.00 | 37.19 | 60.00 | 57.14 | 66.67 | 63.41 | |
| 2 | 46.67 | 44.17 | 46.67 | 42.85 | 60.00 | 56.09 | ||
| 3 | 33.33 | 30.21 | 46.67 | 42.85 | 66.67 | 63.41 | ||
| On average | 40.00 | 37.19 | 51.11 | 47.61 | 64.45 | 60.97 | ||
| On average | 37.67 | 34.86 | 55.55 | 52.37 | 68.89 | 65.88 |
As can be seen from Table 4: Success has certain control efficiency to diamond-back moth, but its control efficiency only is 48～66%; With Success be diluted to 9000 times still little to the difference on effect of diamond-back moth control 6000 times the time.
Table 5 Bt20 and success are mixed and the corrected mortality of diamond-back moth when using separately
| Handle | Concentration | Lethality (%) | Corrected mortality (%) | ||||
| 24h | 48h | 72h | 24h | 48h | 72h | ||
| Bt20 + Success | 1.0×10 8/ mL+6000 doubly | 76.7 | 90.0 | 100 | 76.3 | 89.1 | 100 |
| 1.0×10 8/ mL+9000 doubly | 71.7 | 83.3 | 98.3 | 71.2 | 81.8 | 98.1 | |
| 5×10 7/ mL+6000 doubly | 68.3 | 81.7 | 93.3 | 67.8 | 80.0 | 92.4 | |
| 5×10 7/ mL+9000 doubly | 63.3 | 78.3 | 91.7 | 62.7 | 76.3 | 90.6 | |
| Success | 6000 times | 35.0 | 63.3 | 78.3 | 33.9 | 60.0 | 75.5 |
| 9000 times | 28.3 | 55.0 | 61.7 | 27.1 | 50.9 | 56.8 | |
| Bt20 | 1.0×10 8/mL | 43.3 | 75.0 | 85.0 | 42.3 | 72.7 | 83.1 |
| 5×10 7/mL | 31.7 | 56.7 | 73.3 | 30.5 | 52.8 | 69.9 | |
| Water | - | 1.7 | 8.3 | 11.3 | - | - | - |
As can be seen from Table 5: the insecticidal effect that Bt20 and success are mixed obviously is better than the independent use of independent Bt20 and success; Simultaneously, also as can be seen Success be diluted to 9000 times still 6000 times mixes with Bt20 and to use not quite the control efficiency influence of diamond-back moth.
Sub-district, table 6 field bacterial strain Bt20 and Success use the control efficiency to the diamond-back moth larva with
| Handle | Concentration | Lethality (%) | Corrected mortality (%) | ||||
| 24h | 48h | 72h | 24h | 48h | 72h | ||
| Bt20+success | 1.0×10 8/ mL+6000 doubly | 44.2 | 72.5 | 92.5 | 40.2 | 69.4 | 91.2 |
| 1.0×10 8/ mL+9000 doubly | 38.3 | 61.7 | 84.2 | 33.9 | 57.4 | 81.4 | |
| success | 6000 times | 29.2 | 54.2 | 63.3 | 24.1 | 49.1 | 56.9 |
| 9000 times | 26.7 | 48.3 | 55.8 | 21.4 | 42.6 | 48.0 | |
| Bt20 | 1.0×10 8/mL | 31.7 | 59.2 | 74.2 | 26.8 | 54.6 | 69.6 |
| 2.5% cyhalothrin | 2000 | 47.5 | 54.2 | 60.0 | 43.7 | 49.1 | 52.9 |
| Water | 6.7 | 10.0 | 15.0 | - | - | - |
As can be seen from Table 6: the effect of the control diamond-back moth larva that bacillus thuringiensis Bt20 and success are mixed obviously is better than the independent use of independent Bt20 and success; And, obviously be better than diamond-back moth insecticide 2.5% cyhalothrin of using always.Simultaneously, also as can be seen success be diluted to 9000 times still 6000 times mixes with Bt20 and to use not quite the control efficiency influence of diamond-back moth.
3, the indoor insecticidal activity of biological insecticides and field control effect (table 7～9):
With complex preparation that present embodiment is prepared important agriculture and forestry injurious insects such as cabbage caterpillar, tomato moth, beet webworm have been carried out insecticidal activity assay.The result shows, the cabbage caterpillar preventive effect is reached 89.1% (72h), tomato moth 84.8% (72h), beet webworm 86.7% (48h).Outdoor potted plant and small plot experiment, to 2 age tomato moth control efficiency reach 70～100% (96h).
Behind table 7 Bt20 bacterial strain and Success and the additive mixture to the control efficiency of beet webworm
| Handle | Concentration | Lethality (%) | Corrected mortality (%) | ||||
| 24h | 48h | 72h | 24h | 48h | 72h | ||
| Auxiliary agent | 200 times | 6.7 | 8.3 | 11.7 | 6.7 | 8.3 | 8.6 |
| Composite dose | 200 times | 50.0 | 66.7 | 83.3 | 50.0 | 66.7 | 82.8 |
| success+Bt20 | 6000+ 5×10 7/ml | 35.0 | 43.3 | 66.7 | 35.0 | 43.3 | 65.5 |
Annotate: auxiliary agent is meant other composition combination except bacillus thuringiensis Bt20 and success in the prescription.
As can be seen from Table 7: little when making used additives separately to the insecticidal effect of beet webworm, can ignore; The composite dose of insecticidal effect to beet webworm of present embodiment obviously is better than the control efficiency of success+Bt20 to beet webworm.
Sub-district, table 8 field microbial inoculum is to the result that prevents and treats of cabbage caterpillar
| Processing mode | Working concentration | Repeat | The insect population radix | Dead borer population (head) | Lethality (%) | Corrected mortality (%) |
| 24h | 48h | 72h | 24h | 48h | 72h | 24h | 48h | 72h | ||||
| CK (contrast) | - | 1 | 30 | 0 | 2 | 6 | 0 | 6.67 | 20 | - | - | - |
| 2 | 30 | 0 | 0 | 4 | 0 | 0 | 13.3 | - | - | - | ||
| 3 | 30 | 0 | 2 | 3 | 0 | 6.67 | 10 | - | - | - | ||
| On average | 30 | 0 | 1.33 | 4.33 | 0 | 4.44 | 14.4 | - | - | - | ||
| Bt20 | 150 | 1 | 30 | 21 | 27 | 28 | 70 | 90 | 93.33 | 70 | 89.53 | 92.21 |
| 2 | 30 | 19 | 28 | 28 | 63.33 | 93.33 | 93.33 | 63.33 | 93.02 | 92.21 | ||
| 3 | 30 | 20 | 27 | 29 | 66.67 | 90 | 96.67 | 66.67 | 89.53 | 96.10 | ||
| On average | 30 | 20 | 27.33 | 28.33 | 66.67 | 91.11 | 94.44 | 66.67 | 90.70 | 93.51 | ||
| Composite dose | 150 | 1 | 30 | 18 | 28 | 29 | 60 | 93.33 | 96.67 | 60 | 93.02 | 96.10 |
| 2 | 30 | 24 | 28 | 29 | 80 | 93.33 | 96.67 | 80 | 93.02 | 96.10 | ||
| 3 | 30 | 23 | 27 | 28 | 76.67 | 90 | 93.33 | 76.67 | 89.53 | 92.21 | ||
| On average | 30 | 21.67 | 27.67 | 28.67 | 72.22 | 92.22 | 95.56 | 72.22 | 91.86 | 94.81 |
As can be seen from Table 8: in the field plot trial, the composite dose of insecticidal effect to cabbage caterpillar of present embodiment is better than the independent control efficiency of bacterial strain Bt20.
Composite dose of sub-district, field control test of table 9 Bt to vegetable-crop pest-insect
| Handle | Concentration | Controlling object | The insect population rate (%) that goes down | |||
| 24h | 48h | 72h | 96h | |||
| Composite dose | 150 times | Tomato moth | 15.0 | 35.0 | 55.0 | 75.0 |
As can be seen from Table 9: composite dose of present embodiment all has preventive effect preferably to tomato moth.
Claims (8)
1, the biological mixed pesticide of green food vegetable Tanaka pest control is characterized in that described insecticidal compound agent is bacillus thuringiensis Bt and success composite a dose.
2, the biological mixed pesticide of green food vegetable Tanaka pest control according to claim 1 is characterized in that containing among composite dose of every 100ml bacillus thuringiensis Bt gemma number: 2 * 10
9～7 * 10
9Individual, dish is auspiciousness: 0.01～004ml.
3, the biological mixed pesticide of green food vegetable Tanaka pest control according to claim 1 and 2 is characterized in that described bacillus thuringiensis Bt is bacillus thuringiensis Bt20.
4, the biological mixed pesticide of green food vegetable Tanaka pest control according to claim 3, it is characterized in that described bacillus thuringiensis Bt20 on May 11st, 2006 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC NO.1710.
5, the biological mixed pesticide of green food vegetable Tanaka pest control according to claim 1 is characterized in that containing among composite dose of described every 100ml Tween-60 or Tween-80: 2 grams, citric acid: 1～3 gram, methylcellulose: 1～4 gram, diatomite: 0.2～0.4 gram, Sodium Benzoate: 0.5～2 gram, bacillus thuringiensis Bt20 gemma number: 2 * 10
9～7 * 10
9Individual, success:0.01～004ml.
6,, it is characterized in that containing among composite dose of every 100ml Tween-60 or Tween-80: 2 grams, citric acid: 1 gram, methylcellulose: 1 gram, diatomite: 0.2 gram, Sodium Benzoate: 0.5 gram, bacillus thuringiensis Bt20 gemma number: 2 * 10 according to the biological mixed pesticide of the described green food vegetable Tanaka of claim pest control
9Individual, success:0.01ml.
7,, it is characterized in that containing among composite dose of the 100ml Tween-60 or Tween-80: 2 grams, citric acid: 3 grams, methylcellulose: 4 grams, diatomite: 0.4 gram, Sodium Benzoate: 2 grams, bacillus thuringiensis Bt20 gemma number: 7 * 10 according to the biological mixed pesticide of the described green food vegetable Tanaka of claim pest control
9Individual, success:004ml.
8,, it is characterized in that containing among composite dose of every 100ml Tween-60 or Tween-80: 2 grams, citric acid: 1.5 grams, methylcellulose: 2.6 grams, diatomite: 0.3 gram, Sodium Benzoate: 1 gram, bacillus thuringiensis Bt20 gemma number: 4 * 10 according to the biological mixed pesticide of the described green food vegetable Tanaka of claim pest control
9Individual, success:0.02ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610010264 CN1875717A (en) | 2006-07-07 | 2006-07-07 | Biological mixed pesticide for insect control in green vegetables field |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610010264 CN1875717A (en) | 2006-07-07 | 2006-07-07 | Biological mixed pesticide for insect control in green vegetables field |
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| Publication Number | Publication Date |
|---|---|
| CN1875717A true CN1875717A (en) | 2006-12-13 |
Family
ID=37508410
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610010264 Pending CN1875717A (en) | 2006-07-07 | 2006-07-07 | Biological mixed pesticide for insect control in green vegetables field |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010241696A (en) * | 2009-04-01 | 2010-10-28 | Tokyo Univ Of Agriculture & Technology | Method for controlling pest |
| CN103039526A (en) * | 2012-12-28 | 2013-04-17 | 广西安泰化工有限责任公司 | Disinsection composition containing larvatrol |
-
2006
- 2006-07-07 CN CN 200610010264 patent/CN1875717A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010241696A (en) * | 2009-04-01 | 2010-10-28 | Tokyo Univ Of Agriculture & Technology | Method for controlling pest |
| CN103039526A (en) * | 2012-12-28 | 2013-04-17 | 广西安泰化工有限责任公司 | Disinsection composition containing larvatrol |
| CN103039526B (en) * | 2012-12-28 | 2015-07-15 | 广西安泰化工有限责任公司 | Disinsection composition containing larvatrol |
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