CN1868553A - Cervical skin tissue engineering stent and its making method - Google Patents
Cervical skin tissue engineering stent and its making method Download PDFInfo
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- CN1868553A CN1868553A CNA2006100502037A CN200610050203A CN1868553A CN 1868553 A CN1868553 A CN 1868553A CN A2006100502037 A CNA2006100502037 A CN A2006100502037A CN 200610050203 A CN200610050203 A CN 200610050203A CN 1868553 A CN1868553 A CN 1868553A
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Abstract
A dermal tissue-engineered stent for cervix uteri is composed of a circular disc type base, a semi-spherical or projected cylindrical head, and a neck with a central through hole. It has single-layer or double-layer structure. It is prepared through mixing gelatin, chitosan, chitosan derivative-iodine polymer, glycerine and hyaluronic acid, stirring, laying aside for at least 6 hr, loading in mould, and freeze-drying.
Description
Technical field
The present invention relates to a kind of treatment cervicitis, vaginitis and the skin tissue engineering scaffold of reparation inflammation ulcer wound surface and the manufacture method of this support belong to therapeutic material product and manufacture method.
Background technology
Cervicitis and vaginitis are women's common and frequently-occurring diseases, are by due to the mixing pathogenic flora.All there is unicity in the medicine of antibiotic at present, antiviral, antifungal, anti-chlamydia class, and whole body and topical therapeutic are not good, and wound is caused in the naturopathy part, and also outbreak repeatedly.And medicine and naturopathy all destroyed the ecology of vagina and wound and needed special installation, and the patient is caused mental maladjustment, and tissue injury, tissue have the cicatrixization may.And the antibacterial activity that chitosan and derivant thereof have, anti-tumor activity, increase immunity, promote wound healing, fibrosis, the compatibility is good, nontoxic, can fall Jie, obtain the consistent of scientific circles, medical circle and known together, also more and more show its superiority as a kind of novel antibacterial biological material.Because of the special anatomical features of system genitale under its women, the method for liner targeted therapy of no use all the time, and the tissue engineering bracket that can fall Jie is done the treatment of cervix uteri liner type.
Summary of the invention:
The object of the present invention is to provide a kind of cervical skin tissue engineering stent and manufacture method for the treatment of cervicitis, vaginitis and repairing the inflammation ulcer wound surface, thereby make in inflammation and ulcer spot and obtain skim and its cervical orifice, the close tight adhesion of compliance in cervical tissue surface, and have certain supporting role have sterilization, the short biological reduction of media polymer of repairing and with the compound product of silica gel, and make it in dissipation, finish reproducing fully of tissue surface in the repair process.
Technical scheme of the present invention is: according to the pathological characteristic of Cervical dissect physiology characteristics of women's following reproductive system and cervicitis, invented the antibiotic property biocompatibility that utilizes chitosan and derivant-shell iodo-complexes thereof, utilize collagen and hyaluronic acid, make fallen Jie's who fully complies with adhesion and cervix uteri and cervix uteri biopolymer behind the required substrate hinge of the plasticity of glycerol and tissue repair, played the material of the function of slow release dissipation and reparation.It mainly is made of described support with the middle cervical region that links to each other disk bottom (1), hemispherical head and the middle reducing cervical region that links to each other or outstanding column or coniform head, and its center is provided with axially extending bore.
Described support is by monolayer or inside and outside two-layer compound the composition, and the spacing of its cervical region is at 0.6-1.6cm.
Described support mixes the corresponding kenel monolayer of the porous spongy of making support by gelatin, chitosan, chitosan derivant with many compounds, glycerol, the hyaluronic acid of iodine complexation.
Described support can carry out inside and outside two-layer compound and make corresponding composite bed support with the porous spongy internal layer that the polymer of polylactic acid/chitosan derivant and iodine complexation is made.
Polymer, glycerol, the hyaluronic acid of the also available gelatin of support of the present invention, chitosan, chitosan derivant and iodine complexation are mixed and made into the porous spongy skin, and carry out the compound corresponding composite bed support made from internal layer that the material of making liner with silica gel is made.
A kind of method of making cervical skin tissue engineering stent, it be with gelatin, chitosan, chitosan derivant mix with polymer, glycerol, the hyaluronic acid of iodine complexation leave standstill at least 6 hours after stirring after, the porous spongy support is made in lyophilization in stock mould.
The manufacture method of described cervical skin tissue engineering stent, it is 4g carboxymethyl chitosan or using carboxyl chitosan to be dissolved in stirring in the 100ml distilled water drop into the 4g gelatin after 1 hour and stirred 2 hours, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drain bubble, give in the stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization is 6 hours again, becomes monolayer support product.
The method of described cervical skin tissue engineering stent, it is the polymer mixed of polylactic acid, chitosan derivant and iodine complexation to be stirred back lyophilization in stock mould be shaped to internal layer, and places special-purpose stock mould; Reuse gelatin, chitosan, chitosan derivant mix with polymer, glycerol, the hyaluronic acid of iodine complexation leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
The manufacture method of described cervical skin tissue engineering stent, it is that the 1g polylactic acid is situated between to gel with dichloromethane is molten, the polymer that adds 0.2g carboxymethyl chitosan and 0.4g chitosan derivant and iodine complexation adds after 2g 40-60 order PEO fully stirs again, inject stock mould,-60 ℃ of following pre-freezes 6 hours, lyophilization is 6 hours again, inserts in the special-purpose stock mould after the molding as internal layer again; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound
A kind of method of making cervical skin tissue engineering stent, it is earlier the silica gel liner of molding to be inserted in the product approval mould; Reuse gelatin, chitosan, chitosan derivant mix with polymer, glycerol, the hyaluronic acid of iodine complexation leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
Cervical epithelial tissue engineering rack mechanism of the present invention is: the chitosan class is that the Organic substance of the unique positively charged of nature can adsorb antibacterial one cell membrane and cytoplasmic albumen anion, change the permeability of cell membrane, disturbed cytoplasmic normal physiological to synthesize the bacteriostasis and sterilization effect of having played.The polymer of chitosan derivant and iodine complexation is chitosan and I conjugate, discharges the bactericidal action that the free-iodine element plays iodine when molten Jie.Materials such as gelatin, hyaluronic acid, chitosan have the favorable tissue compatibility, and are the ideal materials of epithelial cell epimatrix, help epithelial absorption and growth.
The present invention has designed and cervix uteri and the good product form of cervicitis compliance, has both played the targeted therapy effect, has played supporting role again, can be effective material inflammation and ulcer are played slow release sterilization and repair.
The present invention has designed biological reduction of media polymer and silica gel liner joint product, keeps the active function of biological reduction of media polymer at tissue surface, has strengthened supporting role again, and the treatment timeliness is guaranteed.
The present invention obtains the outer biological reduction of media polymer liner of therapeutic intracavity that skim is complied with tight adhesion closely with wall above it and tube wall tissue surface and certain supporting role is arranged in inflammation and ulcer spot, makes it inflammation and ulcer surface and is sterilizing, urging to reproduce in the repair process at this tissue surface.
Description of drawings
Fig. 1 is a monolayer supporting structure sketch map of the present invention.
Fig. 2 is a double-layer scaffold structural representation of the present invention.
Fig. 3 is another double-layer scaffold structural representation of the present invention.
The specific embodiment
The present invention will be described in detail below in conjunction with drawings and Examples: shown in the accompanying drawing, it mainly is made of support of the present invention with the middle cervical region 3 that links to each other disk bottom 1, hemispherical head and the middle reducing cervical region that links to each other or outstanding column or coniform head 2, as shown in Figure 1, its center is provided with axially extending bore 4.Described support is by monolayer or inside and outside two-layer compound the composition, and the spacing of its cervical region is at 0.6-1.6cm.
Shown in Figure 1, support of the present invention mixes the corresponding kenel monolayer of the porous spongy of making support by gelatin/chitosan/chitosan derivant with the polymer/glycerol/hyaluronic acid of iodine complexation.
Shown in Figure 2, support of the present invention also can be mixed and made into porous spongy with the polymer/glycerol/hyaluronic acid of gelatin/chitosan/chitosan derivant and iodine complexation by skin, and carries out inside and outside two-layer compound and make corresponding composite bed support with the porous spongy internal layer made from the polymer of polylactic acid/chitosan derivant and iodine complexation.
Shown in Figure 3, support of the present invention can also be mixed and made into the porous spongy skin with the polymer/glycerol/hyaluronic acid of gelatin/chitosan/chitosan derivant and iodine complexation, and carries out the compound corresponding composite bed support made from internal layer that the material of making liner with silica gel is made.
Embodiment one, it is with the 4g carboxymethyl chitosan, and using carboxyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drops into glycerol 1g stirring again and carries out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drained bubble, give in the stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization was 6 hours again, became monolayer support product.
Embodiment two, it is situated between the 1g polylactic acid to gel with dichloromethane is molten, the polymer that adds 0.2g carboxymethyl chitosan and 0.4g chitosan derivant and iodine complexation adds after 2g 40-60 order PEO fully stirs again, inject stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization is 6 hours again, inserts in the special-purpose stock mould after the molding as internal layer again; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound
Embodiment three, and it is inserted the silica gel liner of molding in the product approval mould earlier; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound.
The present invention be the characteristics according to cervical canal designed this support head greater than cervical region to play the support effect of playing, simultaneously in the middle of perforate so that cervical canal secretions is drained, cervical region and cervical department are according to tight sticking inflammation of physiology design and ulcer spot.Two layers of support have been designed again.Skin fell Jie at 7-10 days, and internal layer polylactic acid, chitosan and chitosan iodine fell Jie at 30-60 days, and the compliance outside having supported has simultaneously kept the unobstructed property of endoporus again and do not released by Excreta, made it to remain on cervical canal interior 30-60 days.Simultaneously in order to keep molecular structure to play a role in target site, and guarantee that effective treatment timeliness has designed the polymer of outer chitosan derivant and iodine complexation again, internal layer is that silica gel is made the joint product that gasket material is formed, and provides a new product and new treatment technology method for treating women's cervicitis and vaginitis.
Claims (10)
1, a kind of cervical skin tissue engineering stent, it is characterized in that it mainly is made of with the middle cervical region (3) that links to each other disk bottom (1), hemispherical head and the middle reducing cervical region (2) that links to each other or outstanding column or coniform head, its center is provided with axially extending bore (4).
2, cervical skin tissue engineering stent according to claim 1 is characterized in that described support by monolayer or inside and outside two-layer compound the composition, and the spacing of its cervical region is at 0.6-1.6cm.
3, cervical skin tissue engineering stent according to claim 1 and 2 is characterized in that described support mixes the corresponding kenel monolayer of the porous spongy of making support by gelatin, chitosan, chitosan derivant with many compounds, glycerol, the hyaluronic acid of iodine complexation.
4, cervical skin tissue engineering stent according to claim 1 and 2, it is inside and outside two-layer compound and make corresponding composite bed support to it is characterized in that described support can carry out with the porous spongy internal layer that the polymer of polylactic acid/chitosan derivant and iodine complexation is made.
5, cervical skin tissue engineering stent according to claim 1 and 2, the polymer, glycerol, the hyaluronic acid that it is characterized in that the also available gelatin of support of the present invention, chitosan, chitosan derivant and iodine complexation are mixed and made into the porous spongy skin, and carry out the compound corresponding composite bed support made from internal layer that the material of making liner with silica gel is made.
6, a kind of method of making as claim 1 or 2 or 3 described cervical skin tissue engineering stents, it be with gelatin, chitosan, chitosan derivant mix with polymer, glycerol, the hyaluronic acid of iodine complexation leave standstill at least 6 hours after stirring after, the porous spongy support is made in lyophilization in stock mould.
7, the manufacture method of cervical skin tissue engineering stent according to claim 6, it is 4g carboxymethyl chitosan or using carboxyl chitosan to be dissolved in stirring in the 100ml distilled water drop into the 4g gelatin after 1 hour and stirred 2 hours, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drain bubble, give in the stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization is 6 hours again, becomes monolayer support product.
8, a kind of method of making as claim 1 or 2 or 4 described cervical skin tissue engineering stents, it is the polymer mixed of polylactic acid, chitosan derivant and iodine complexation to be stirred back lyophilization in stock mould be shaped to internal layer, and places special-purpose stock mould; Reuse gelatin, chitosan, chitosan derivant mix with polymer, glycerol, the hyaluronic acid of iodine complexation leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
9, the manufacture method of cervical skin tissue engineering stent according to claim 8, it is that the 1g polylactic acid is situated between to gel with dichloromethane is molten, the polymer that adds 0.2g carboxymethyl chitosan and 0.4g chitosan derivant and iodine complexation adds after 2g 40-60 order PEO fully stirs again, inject stock mould,-60 ℃ of following pre-freezes 6 hours, lyophilization is 6 hours again, inserts in the special-purpose stock mould after the molding as internal layer again; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, the polymer that adds 1g chitosan derivant and iodine complexation again stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound.
10, a kind of method of making as claim 1 or 2 or 5 described cervical skin tissue engineering stents, it is earlier the silica gel liner of molding to be inserted in the product approval mould; Reuse gelatin, chitosan, chitosan derivant mix with polymer, glycerol, the hyaluronic acid of iodine complexation leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100502037A CN1868553A (en) | 2006-04-04 | 2006-04-04 | Cervical skin tissue engineering stent and its making method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100502037A CN1868553A (en) | 2006-04-04 | 2006-04-04 | Cervical skin tissue engineering stent and its making method |
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| CN1868553A true CN1868553A (en) | 2006-11-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2006100502037A Pending CN1868553A (en) | 2006-04-04 | 2006-04-04 | Cervical skin tissue engineering stent and its making method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102886061A (en) * | 2011-07-21 | 2013-01-23 | 武汉市三鼎医用生物制品有限公司 | Chitosan-iodide biological antibacterial film |
| CN102886062A (en) * | 2011-07-21 | 2013-01-23 | 武汉市三鼎医用生物制品有限公司 | Iodinated chitosan biological antibacterial gel |
| CN101543658B (en) * | 2009-04-29 | 2013-03-13 | 上海市计划生育科学研究所 | Cervical cap for preventing and treating cervical erosion and preparation method thereof |
| CN104053459A (en) * | 2011-11-23 | 2014-09-17 | 清华大学 | Method and device for constructing three-dimensional cellular microenvironment on the basis of transparent sponge scaffold |
| CN109303934A (en) * | 2018-12-14 | 2019-02-05 | 嘉兴市舒福特生物科技有限公司 | A kind of bio-medical repair membrane and preparation method thereof |
-
2006
- 2006-04-04 CN CNA2006100502037A patent/CN1868553A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101543658B (en) * | 2009-04-29 | 2013-03-13 | 上海市计划生育科学研究所 | Cervical cap for preventing and treating cervical erosion and preparation method thereof |
| CN102886061A (en) * | 2011-07-21 | 2013-01-23 | 武汉市三鼎医用生物制品有限公司 | Chitosan-iodide biological antibacterial film |
| CN102886062A (en) * | 2011-07-21 | 2013-01-23 | 武汉市三鼎医用生物制品有限公司 | Iodinated chitosan biological antibacterial gel |
| CN104053459A (en) * | 2011-11-23 | 2014-09-17 | 清华大学 | Method and device for constructing three-dimensional cellular microenvironment on the basis of transparent sponge scaffold |
| CN104053459B (en) * | 2011-11-23 | 2016-02-24 | 清华大学 | Method and the device of three-dimensional cell microenvironment is built based on transparent sponge bracket |
| CN109303934A (en) * | 2018-12-14 | 2019-02-05 | 嘉兴市舒福特生物科技有限公司 | A kind of bio-medical repair membrane and preparation method thereof |
| CN109303934B (en) * | 2018-12-14 | 2021-05-07 | 嘉兴市舒福特生物科技有限公司 | Biomedical repair film and preparation method thereof |
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