CN1720876A - Uterus neck skin tissue engineering scaffold and method for producing the same - Google Patents
Uterus neck skin tissue engineering scaffold and method for producing the same Download PDFInfo
- Publication number
- CN1720876A CN1720876A CN 200510049781 CN200510049781A CN1720876A CN 1720876 A CN1720876 A CN 1720876A CN 200510049781 CN200510049781 CN 200510049781 CN 200510049781 A CN200510049781 A CN 200510049781A CN 1720876 A CN1720876 A CN 1720876A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- hours
- cmcts
- paana
- pvp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a uterine neck skin tissue engineering scaffold and its making method, wherein the scaffold mainly comprises a disc-shaped bottom, a hemispherical head and a choked neck connected at the center with a central axial through hole, the scaffold is combined by an inner and an outer layers, the span of the necks is between 0.6-1.6cm. The material for making the scaffold includes gelatin, chitosan, and chitosan derivative CMCTS-g-(PAANa-CO-PVP)-I2, glycerin, and hyaluronic acid.
Description
Technical field
The present invention relates to a kind of treatment cervicitis, vaginitis and the skin tissue engineering scaffold of reparation inflammation ulcer wound surface and the manufacture method of this support belong to therapeutic material product and manufacture method.
Background technology
Cervicitis and vaginitis are women's common and frequently-occurring diseases, are by due to the mixing pathogenic flora.All there is unicity in the medicine of antibiotic at present, antiviral, antifungal, anti-chlamydia class, and whole body and topical therapeutic are not good, and wound is caused in the naturopathy part, and also outbreak repeatedly.And medicine and naturopathy all destroyed the ecology of vagina and wound and needed special installation, and the patient is caused mental maladjustment, and tissue injury, tissue have the cicatrixization may.And the antibacterial activity that chitosan and derivant thereof have, anti-tumor activity, increase immunity, promote wound healing, fibrosis, the compatibility is good, nontoxic, can fall Jie, obtain the consistent of scientific circles, medical circle and known together, also more and more show its superiority as a kind of novel antibacterial biological material.Because of the special anatomical features of system genitale under its women, the method for liner targeted therapy of no use all the time, and the tissue engineering bracket that can fall Jie is done the treatment of cervix uteri liner type.
Summary of the invention:
The object of the present invention is to provide a kind of cervical skin tissue engineering stent and manufacture method for the treatment of cervicitis, vaginitis and repairing the inflammation ulcer wound surface, thereby make in inflammation and ulcer spot and obtain skim and its cervical orifice, the tight adhesion of compliance closely of cervical tissue surface, and have certain supporting role have sterilization, the short biological reduction of media polymer of repairing and with the compound product of silica gel, and make it in dissipation, finish reproducing fully of tissue surface in the repair process.
Technical scheme of the present invention is: according to the pathological characteristic of Cervical dissect physiology characteristics of women's following reproductive system and cervicitis, invented the antibiotic property biocompatibility that utilizes chitosan and derivant thereof, utilize collagen and hyaluronic acid, make fallen Jie's who fully complies with adhesion and cervix uteri and cervix uteri biopolymer behind the required substrate hinge of the plasticity of glycerol and tissue repair, played the material of the function of slow release dissipation and reparation.It mainly is made of described support disk bottom, hemispherical head and the middle reducing cervical region that links to each other, and its center is provided with axially extending bore.
Described support is by monolayer or inside and outside two-layer compound the composition, and the spacing of its cervical region is at 0.6-1.6cm.
Described support is by gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol/hyaluronic acid mixes the corresponding kenel monolayer of the porous spongy support of making.
Described support gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid is mixed and made into the porous spongy skin, and with polylactic acid, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2The porous spongy internal layer of making carries out inside and outside two-layer compound, and makes corresponding composite bed support.
Support of the present invention can also be used chitosan, gelatin, hyaluronic acid, glycerol, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Make skin, and carry out the compound corresponding composite bed support made from internal layer that the material of making liner with silica gel is made.
A kind of method of making foregoing support is with gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid mix leave standstill at least 6 hours after stirring after, the porous spongy support is made in lyophilization in stock mould,
Foregoing manufacture method, it is the 4g carboxymethyl chitosan to be dissolved in the 100ml distilled water stirring drop into the 4g gelatin after 1 hour and stirred 2 hours, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, give in the stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization was 6 hours again, became monolayer support product.
A kind of method of making foregoing support, it is with polylactic acid, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Mix the lyophilization in stock mould of stirring back and be shaped to internal layer, and place special-purpose stock mould; Reuse gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid mix leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
Foregoing manufacture method, it is situated between the 1g polylactic acid to gel with dichloromethane is molten, adds 0.2g carboxymethyl chitosan and 0.4g chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Add after 2g 40-60 order PEO fully stirs again, inject stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization was 6 hours again, inserts in the special-purpose stock mould after the molding as internal layer again; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound
A kind of method of making foregoing support is inserted the silica gel liner of molding in the product approval mould earlier; Reuse gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid mix leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
Foregoing manufacture method is inserted the silica gel liner of molding in the product approval mould earlier; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound.
Cervical epithelial tissue engineering rack mechanism of the present invention is: the chitosan class is that the Organic substance of the unique positively charged of nature can adsorb antibacterial one cell membrane and cytoplasmic albumen anion, change the permeability of cell membrane, disturbed cytoplasmic normal physiological to synthesize the bacteriostasis and sterilization effect of having played.Chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Be chitosan and I conjugate, when molten Jie, discharge the bactericidal action that the free-iodine element plays iodine.Materials such as gelatin, hyaluronic acid, chitosan have the favorable tissue compatibility, and are the ideal materials of epithelial cell epimatrix, help epithelial absorption and growth.
The present invention has designed and cervix uteri and the good product form of cervicitis compliance, has both played the targeted therapy effect, has played supporting role again, can be effective material inflammation and ulcer are played slow release sterilization and repair.
The present invention has designed biological reduction of media polymer and silica gel liner joint product, keeps the active function of biological reduction of media polymer at tissue surface, has strengthened supporting role again, and the treatment timeliness is guaranteed.
The present invention obtains the outer biological reduction of media polymer liner of therapeutic intracavity that skim is complied with tight adhesion closely with wall above it and tube wall tissue surface and certain supporting role is arranged in inflammation and ulcer spot, makes it inflammation and ulcer surface and is sterilizing, urging to reproduce in the repair process at this tissue surface.
Description of drawings
Fig. 1 is a monolayer supporting structure sketch map of the present invention.
Fig. 2 is a double-layer scaffold structural representation of the present invention.
Fig. 3 is another double-layer scaffold structural representation of the present invention.
The specific embodiment
The present invention will be described in detail below in conjunction with drawings and Examples: shown in the accompanying drawing, support of the present invention mainly is made of disk bottom 1, hemispherical head 2 and the middle reducing cervical region 3 that links to each other, and its center is provided with axially extending bore 4.Described support is by monolayer or inside and outside two-layer compound the composition, and the spacing of its cervical region is at 0.6-1.6cm.
Shown in Figure 1, support of the present invention is by gelatin/chitosan/chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2/ glycerol/hyaluronic acid mixes the corresponding kenel monolayer of the porous spongy support of making.
Shown in Figure 2, support of the present invention also can be by skin with gelatin/chitosan/chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2/ glycerol/hyaluronic acid is mixed and made into porous spongy, and with polylactic acid/chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2The porous spongy internal layer of making carries out inside and outside two-layer compound and make corresponding composite bed support.
Shown in Figure 3, support of the present invention can also be used gelatin/chitosan/chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2/ glycerol/hyaluronic acid is mixed and made into the porous spongy skin, and carries out the compound corresponding composite bed support made from internal layer that the material of making liner with silica gel is made.
Embodiment one, it is the 4g carboxymethyl chitosan to be dissolved in the 100ml distilled water stirring drop into the 4g gelatin after 1 hour and stirred 2 hours, drop into glycerol 1g stirring again and carried out hinge in 2 hours, added hyaluronic acid again 2 hours, add 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, give in the stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization was 6 hours again, became monolayer support product.
Embodiment two, and it is situated between the 1g polylactic acid to gel with dichloromethane is molten, add 0.2g carboxymethyl chitosan and 0.4g chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Add after 2g 40-60 order PEO fully stirs again, inject stock mould, subzero pre-freeze below 50 ℃ 6 hours, lyophilization was 6 hours again, inserts in the special-purpose stock mould after the molding as internal layer again; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound
Embodiment three, and it is inserted the silica gel liner of molding in the product approval mould earlier; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound.
The present invention be the characteristics according to cervical canal designed this support head greater than cervical region to play the support effect of playing, simultaneously in the middle of perforate so that cervical canal secretions is drained, cervical region and cervical department are according to tight sticking inflammation of physiology design and ulcer spot.Two layers of support have been designed again.Skin fell Jie at 7-10 days, and internal layer polylactic acid, chitosan and chitosan iodine fell Jie at 30-60 days, and the compliance outside having supported has simultaneously kept the unobstructed property of endoporus again and do not released by Excreta, made it to remain on cervical canal interior 30-60 days.Simultaneously in order to keep molecular structure to play a role, and guarantee that effectively the treatment timeliness has designed outer chitosan class and derivant CMCTS-g-(PAANa-CO-PVP)-I thereof again in target site
2, internal layer is that silica gel is made the joint product that gasket material is formed, and provides a new product and new treatment technology method for treating women's cervicitis and vaginitis.
Claims (10)
1, a kind of cervical skin tissue engineering stent is characterized in that it mainly is made of disk bottom (1), hemispherical head (2) and the middle reducing cervical region (3) that links to each other, and its center is provided with axially extending bore (4).
2, cervical skin tissue engineering stent according to claim 1 is characterized in that described support by monolayer or inside and outside two-layer compound the composition, and the spacing of its cervical region is at 0.6-1.6cm.
3, cervical skin tissue engineering stent according to claim 1 and 2 is characterized in that described support is by gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid mixes the corresponding kenel monolayer of the porous spongy support of making.
4, cervical skin tissue engineering stent according to claim 1 and 2, it is characterized in that described support also can and with polylactic acid/chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2The porous spongy internal layer of making carries out inside and outside two-layer compound and make corresponding composite bed support.
5, cervical skin tissue engineering stent according to claim 1 and 2 is characterized in that support of the present invention can also use gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid is mixed and made into the porous spongy skin, and carries out the compound corresponding composite bed support made from internal layer that the material of making liner with silica gel is made.
6, a kind of method of making as claim 1 or 2 or 3 described cervical skin tissue engineering stents, it is with gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO PVP)-I
2, glycerol, hyaluronic acid mix leave standstill at least 6 hours after stirring after, the porous spongy support is made in lyophilization in stock mould.
7, the manufacture method of cervical skin tissue engineering stent according to claim 6, it is the 4g carboxymethyl chitosan to be dissolved in the 100ml distilled water stirring drop into the 4g gelatin after 1 hour and stirred 2 hours, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, give in the stock mould, the following pre-freeze of subzero 50C 6 hours, lyophilization was 6 hours again, became monolayer support product.
8, a kind of method of making as claim 1 or 2 or 4 described cervical skin tissue engineering stents, it is with polylactic acid, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Mix the lyophilization in stock mould of stirring back and be shaped to internal layer, and place special-purpose stock mould; Reuse gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid mix leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
9, the manufacture method of cervical skin tissue engineering stent according to claim 8, it is that the 1g polylactic acid is situated between to gel with dichloromethane is molten, adds 0.2g carboxymethyl chitosan and 0.4g chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2Add again after 2g 40-60 order PEO fully stirs, inject stock mould, pre-freeze (60 degree) 6 hours, lyophilization is 6 hours again, inserts in the special-purpose stock mould after the molding as internal layer again; Again the 4g carboxymethyl chitosan is dissolved in stirring in the 100ml distilled water and drops into 4g gelatin stirring 2 hours after 1 hour, drop into glycerol 1g stirring again and carried out hinge in 2 hours, add hyaluronic acid again 2 hours, and added 1g chitosan derivatives CMCTS-g-(PAANa-CO-PVP)-I again
2Stirred 1 hour, left standstill 12 hours, drain bubble, inject that lyophilization becomes the porous spongy skin on the internal layer of above-mentioned special-purpose stock mould, and with and make corresponding composite bed support with internal layer is compound
10, a kind of method of making as claim 1 or 2 or 5 described cervical skin tissue engineering stents, it is earlier the silica gel liner of molding to be inserted in the product approval mould; Reuse gelatin, chitosan, chitosan derivant CMCTS-g-(PAANa-CO-PVP)-I
2, glycerol, hyaluronic acid mix leave standstill at least 6 hours after stirring after, the porous spongy skin is made in lyophilization in this special use stock mould, and makes corresponding composite bed support with internal layer is compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510049781 CN1720876A (en) | 2005-05-10 | 2005-05-10 | Uterus neck skin tissue engineering scaffold and method for producing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510049781 CN1720876A (en) | 2005-05-10 | 2005-05-10 | Uterus neck skin tissue engineering scaffold and method for producing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1720876A true CN1720876A (en) | 2006-01-18 |
Family
ID=35911733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510049781 Pending CN1720876A (en) | 2005-05-10 | 2005-05-10 | Uterus neck skin tissue engineering scaffold and method for producing the same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1720876A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101073325B (en) * | 2006-05-19 | 2011-02-16 | 陈煜� | Povidone-iodine antibacterial material and its production |
| CN103239758A (en) * | 2012-02-14 | 2013-08-14 | 深圳兰度生物材料有限公司 | Artificial dermis bracket and preparation method of artificial dermis bracket |
| CN102002132B (en) * | 2009-09-02 | 2014-05-28 | 陈煜� | Improvement on method for preparing complex of polyvinylpyrrolidone grafted with natural polymer or water-soluble derivate thereof and iodine derivate |
-
2005
- 2005-05-10 CN CN 200510049781 patent/CN1720876A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101073325B (en) * | 2006-05-19 | 2011-02-16 | 陈煜� | Povidone-iodine antibacterial material and its production |
| CN102002132B (en) * | 2009-09-02 | 2014-05-28 | 陈煜� | Improvement on method for preparing complex of polyvinylpyrrolidone grafted with natural polymer or water-soluble derivate thereof and iodine derivate |
| CN103239758A (en) * | 2012-02-14 | 2013-08-14 | 深圳兰度生物材料有限公司 | Artificial dermis bracket and preparation method of artificial dermis bracket |
| CN103239758B (en) * | 2012-02-14 | 2015-01-21 | 深圳兰度生物材料有限公司 | Artificial dermis bracket and preparation method of artificial dermis bracket |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Carvalho et al. | Latest advances on bacterial cellulose‐based materials for wound healing, delivery systems, and tissue engineering | |
| Rahmani Del Bakhshayesh et al. | Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering | |
| Qasim et al. | Advancements and frontiers in nano-based 3D and 4D scaffolds for bone and cartilage tissue engineering | |
| Biswas et al. | Recent advancement of biopolymers and their potential biomedical applications | |
| Yadav et al. | Chitosan-based 3D-printed scaffolds for bone tissue engineering | |
| Khan et al. | Fabrication strategies and biomedical applications of three-dimensional bacterial cellulose-based scaffolds: A review | |
| RU2491961C2 (en) | Artificial dura mater and method of its production | |
| CN105705172B (en) | Hydrophilic electrostatic spinning biological composite scaffold material for tissue regeneration and preparation method and application thereof | |
| Azari et al. | Electrospun polycaprolactone nanofibers: Current research and applications in biomedical application | |
| CN102078639A (en) | Medicine carrying method of medical sponge | |
| Zhang et al. | Natural polymer‐derived bioscaffolds for peripheral nerve regeneration | |
| CN101219241A (en) | Biological activity bone renovation material with bone-inducing factor control-release function and production method thereof | |
| Same et al. | Halloysite clay nanotube in regenerative medicine for tissue and wound healing | |
| Bouhlouli et al. | Applications of bacterial cellulose as a natural polymer in tissue engineering | |
| Yang et al. | The application of natural polymer–based hydrogels in tissue engineering | |
| CN1868553A (en) | Cervical skin tissue engineering stent and its making method | |
| CN109731130A (en) | A kind of method that low-temperature biological 3D printing technique prepares hydrogel Wound dressing | |
| CN109771693A (en) | A kind of preparation method for the new injectable spontaneous coagulation cmposite artificial bone carrying rhBMP_2 microballoon | |
| Alvarez Echazu et al. | Recent advances in synthetic and natural biomaterials‐based therapy for bone defects | |
| Yang et al. | Photocrosslinked methacrylated natural macromolecular hydrogels for tissue engineering: A review | |
| Zhang et al. | Application of chitosan with different molecular weights in cartilage tissue engineering | |
| CN1720876A (en) | Uterus neck skin tissue engineering scaffold and method for producing the same | |
| Damiri et al. | Chitosan nanocomposites as scaffolds for bone tissue regeneration | |
| Ölmez et al. | Chitosan and alginate scaffolds for bone tissue regeneration | |
| Uddin et al. | Tissue engineering and the potential use of chitin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |