CN1858184A - Extracting carbon dioxide, polypeptide feed, nucleic acid, amino acid and fermented wine from grain fermentation - Google Patents
Extracting carbon dioxide, polypeptide feed, nucleic acid, amino acid and fermented wine from grain fermentation Download PDFInfo
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- CN1858184A CN1858184A CNA2006100713818A CN200610071381A CN1858184A CN 1858184 A CN1858184 A CN 1858184A CN A2006100713818 A CNA2006100713818 A CN A2006100713818A CN 200610071381 A CN200610071381 A CN 200610071381A CN 1858184 A CN1858184 A CN 1858184A
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Abstract
The process of preparing CO2, polypeptide feed, nucleic acid, amino acid and fermented wine from fermented grains includes fermenting rice, corn, kaoliang and/or glutinous rice with high quality yeast through clear liquid fermentation to obtain fermented liquid with rich active peptide, amino acid, nucleic acid, etc. and distiller's grains with rich peptide, amino acid, nucleic acid and vitamins; and other extracting steps.
Description
(technical field)
The invention belongs to field of deep processing of grains
(background technology)
Modern scientific research is found, with the nourishing body of grain as yeast increment fermentation, starch, protein, VITAMIN, trace element in the grain can effectively be transformed, and greatly improve the zymic value added on products.Finally the product of the two is active polypeptide feed, nucleic acid, amino acid, carbonic acid gas, fermented wine products such as (gin).Above-mentioned these products are for the mankind's health care, animal rearing, drink and have significant function to human health.But owing to active polypeptide feed, nucleic acid, amino acid, carbonic acid gas, fermented wine complex manufacturing such as (gin), the technical difficulty height, some manufacturing and processing enterprises generally all adopt the simple production technology of one, two principal item.Chinese patent application 99112068.X " a kind of nucleic acid production technique ", 96103663X " the cereuisiae fermentum full-enzyme method extracts method of compound amino-acid ", " 01106512.5 from cereuisiae fermentum, extracting the method for Nucleotide ", " 200510053216.5 a kind of low alcohol content white spirit brewed in completely fermenting process ", " 200510119644.3 a kind of gin and production method thereof ", and traditional distilling liquor, the fermentation rice wine production.After aforesaid method all causes major product function goods and materials to extract, in waste residue, waste water, still retain different types of other useful nutrition goods and materials, what have can only be used as normal diet, these useful nutrition goods and materials effectively do not extract and use, can increase the content of organic pollutant in waste residue, the waste water, not only cause the wasting of resources, single product production cost height, caused the environmental protection cost to improve, the market competitiveness is not strong yet.
(summary of the invention)
The present invention is not the simple combination of foregoing invention, but will remedy the deficiency that prior art can only be extracted a small amount of kind, production cost height, waste resource and contaminate environment in grain and the yeast saccharomyces cerevisiae.Adopt grain fermentation back one-time continuous to extract the production method of active polypeptide feed, nucleic acid, amino acid, carbonic acid gas, the multiple composition of fermented wine (gin).
The production method of multiple composition.
For addressing the above problem, the present invention adopts following method to make:
1, fermentation
(a) with grain, rice, corn, jowar, glutinous rice shelling (skin) are pulverized;
(b) with after the above-mentioned grain gelatinization, enter fermentation and irritate, add song simultaneously, add yeast, the fermentation of saccharification limit, limit changed secondary fermentation over to after 5-7 days, and secondary fermentation in 27-30 days finishes;
2, separate
(a) with whizzer with the yeast separation in the fermented liquid, with pulling the frame pressure filter with the fermented liquid solid-liquid separation.
3, extract
(a) carbonic acid gas.Irritate the upper end in fermentation carbon dioxide recovery equipment is installed.
(b) active polypeptide feed.Solid-state goods and materials (vinasse) after the solid-liquid separation contain the peptide class, according to after the proprietary feed formulation adjustment, use the special-purpose two-stage air stream drying of fermented feed unit mahine, make drying effect reach best.
(c) nucleic acid.
(c) 1, cytoclasis.The isolated yeast of whizzer is washed, adds water with clear water in yeast, with the pH value 4.5 of acid with mixture, feed 45～55 ℃ of hot water thermal insulatings, yeast autolysis 24 hours, in autolysing yeast, add 0.20% prozyme, carrying out the yeast cell fragmentation through nanometer ram machine, is 97.9 through check cytoclasis filter, gets the yeast miscellany;
(c) 2, lixiviate.Above-mentioned yeast mixt is put into container, add the extracting solution of 54～58 ℃ of preheatings, (10% cetyl trimethylammonium bromide＜CTAB〉0.14Mol/L naci solution), centrifugal in 54～58 ℃ of stirrings 30 minutes, get supernatant liquor.In above-mentioned throw out, add the extracting solution of 54～58 ℃ of preheatings again, repeat above-mentioned lixiviate, centrifugal, get supernatant liquor;
(C) 3, alcohol precipitation drying.With the supernatant liquor of above-mentioned extracted twice, add ethanol and make and contain the alcohol amount and reach 70%, the collecting precipitation thing, drying promptly gets composite nucleic acid (DNA, RNA);
(D) amino acid
(D) 1, size mixing.With 1: 2 ratio furnishing slurries, pH value was adjusted into 6.3～6.9, heats 60～62 ℃, 30 minutes with (C) throw out;
(D) 2, hydrolysis.Add proteolytic enzyme, α-Dian Fenmei, beta-amylase, stirring is mixed, stirs once, and is hydrolyzed under the 40-50 temperature, till molecular weight is less than 3000 dalton in 4 hours;
(D) 3, press filtration.Hydrolyzed solution is carried out press filtration under 120 order filter clothes, filtrate enters concentrating pan, and to be evaporated to solid content be 20～30%, and vapor pressure is the 1-2 kg/cm, obtains compound amino acid primary liquid;
(D) 4, powder process.With the aminoacids solution of concentrated solution with the spray-dryer drying of dusting, amino acid dry powder.
(E) fermented wine (gin)
(E) 1, fermentation.With grain shelling (skin), take off embryo, cleaning, pulverize the back gelatinization, be cooled to 28 degree and enter fermentation and irritate, add song (liquor song) and add αDian Fenmei after 3 hours and carry out proteolysis, add three primary yeasts after 1.5 hours, fermentation.Temperature entered secondary fermentation afterwards in 4 days at 28～32 ℃, and temperature is controlled at 11～14 degree, gets new wine after 30 days.
(E) 2, arrangement: new wine is accelerated the ripening with high-frequency electronic concussion instrument, the method that adopts ozone oxidation, wine to combine then with complex type molecular sieve of macromolecular material etc., remove in the wine, materials such as potato spirit, ethyl oleate, ethyl linoleate, ethyl palmitate, filter then new wine.
(E) 3, urge old.Adopt the drinks catalytic aging technology of Tianjin Nankai University.This technical matters flow process is as follows:
New wine → wine pump → preheater → ager → water cooler → ripe wine.
The present invention has following characteristics: 1, the present invention is not the simple combination of prior art, but the innovation that grain deep processing industry develops to high technology content high added value direction.In the past, the byproduct after grain brews alcoholic beverages just is used as general feeds, and price per ton only is 2000 yuan.And the present invention is with the nourishing body of grain as yeast increment fermentation, by adding distiller's yeast, make the starch in the grain be converted into sugar, protein transforms and is Shi, peptone, peptide, amino acid, add yeast then, impel yeast cell to breed with geometricprogression, and above-mentioned nutritive substance is converted into wine, polypeptide class, nucleic acid, amino acid, carbonic acid gas, VITAMIN etc., 1kg rice produces 3kg fermented liquid, 0.25kg solid (vinasse), 1 kilogram of fermented liquid produces the 10.56g dry yeast, nucleic acid content 8～15 in the yeast, aminoacids content 30～40%.Adopt brewed in completely fermenting process, guaranteed that complete nutrients matter all is retained in the fermented liquid., the particularly existence of a large amount of active polypeptide class materials.By the one time fermentation of grain, just can extract carbonic acid gas, active polypeptide feed, nucleic acid, amino acid, fermented wine (gin) continuously, remarkable in economical benefits, 70,000 yuan of the every processing one ton-grain food of this technology output values, 3.7 ten thousand yuan of tax revenues, 2.3 ten thousand yuan of profits; 2, produce the product that traditional production technique can't be produced.The production method of traditional liquor is that grain fermentation back distillation is obtained product, and the ethanol content in the former wine is at 55 °～60 °.And consumer wants liquor below 40 ° in recent years, traditional processing technology is irreversible, and manufacturer have to take alcohol to add water and blend low alcohol white spirit, and this wine has no nutrition.The low alcohol white spirit that production technique of the present invention is directly brewageed, former wine alcoholic strength is about 20 degree, no matter the mouthfeel and the nutritional quality in the product that are product all are best, filled up the blank that China can't the direct production low alcohol white spirit, made common cereal product processing enter the high-tech ranks of modern biotechnology; 3, according to existing patented technology inquiry, grain processing can only be produced two kinds of goods and materials, and all the other goods and materials can't produce owing to the fermentation process difference or content is low remains in waste residue, the waste water.The present invention extracts carbonic acid gas, active polypeptide feed, nucleic acid, amino acid, fermented wine five kinds of products such as (gin) continuously from the grain one time fermentation transforms, make and found the basic decomposition and inversion of material grain at present, production cost reduces, and waste obtains comprehensive utilization, helps environment protection; 4, utilization ratio is low and become waste each other between the product produced of prior art.For example: traditional distilling liquor, when fermentation, also produced a large amount of active yeasts, amino acid, VITAMIN, and the high temperature when distillation is really killed live yeast and amino acid, product does not have any nutrition, and its vinasse also can only be used as normal diet.Such mode of production causes the waste of resource, the production cost height; 5, the present invention is the nutrition goods and materials that have according to grain, also is that microorganism growth is needed, and aspect the ratio of grain, the selection utilization of microorganism is suitable, all exists each other between five kinds of goods and materials that extracted, and has optimum performance separately.Carbonic acid gas produces nature and extracts when fermentation, yeast is the product when high gravity fermentation, nucleic acid, amino acid in the yeast independently can extract respectively separately, the peptidic substrate money and the dead yeast that produce during fermentation all are retained in the solid (vinasse), and fermented wine (gin) promptly contains the abundant mellow again soft Qu Xiang of having of nutrition goods and materials mouthfeel, Mi Xiang, the taste of sweet-smelling.This production method has promptly solved the low problem of grain deep processing added value, and having solved traditional liquor does not again have the difficult problem that nutrition is hurted sb.'s feelings.Change people and thought that always grain processing can only make wine or make the traditional concept of feed, finally made grain " eat do press clean " not have waste substantially and generate, reduced the content of organic pollutant, except that improving resource utilization, also reduced the environmental protection cost.The present invention compares with existing grain deep processing, and its economic benefit increases substantially.
Description of drawings: Fig. 1 is a process flow sheet of the present invention.
Specific implementation method:
1, fermentation.
(a) with rice, corn, jowar, glutinous rice, in proportion 60%, 20%, 10%, 10%, grain shells (skin), takes off embryo, cleaning, pulverize the back gelatinization, be cooled to 28 degree and enter the fermentation filling, add song (liquor song) and add αDian Fenmei after 3 hours and carry out proteolysis, add three primary yeasts after 1.5 hours, fermentation.Temperature entered secondary fermentation afterwards in 4 days at 28～32 ℃, and temperature is controlled at 11～14 degree, gets new wine after 30 days.
2, centrifuge dewatering is used in the yeast extraction of fermented liquid bottom, with pulling the frame filter with the fermented liquid solid-liquid separation.
3, extract
(a) carbonic acid gas.Irritate the upper end in fermentation carbon dioxide recovery equipment is installed, comprise scum dredger, air storage bag, washing tower, compressor, remove the composition rate of recovery 100% such as smelling tower, dry liquefaction system, purification system, container for storing liquid, gasification and decompression system, carbonic acid gas can reach and be food grade more than 99.99.
(B) active polypeptide feed.Solid-state goods and materials (vinasse) after the solid-liquid separation, contain peptide class, 18 kinds with upper amino acid, VITAMIN, protein, trace element, after proprietary feed formulation adjustment, use the special-purpose two-stage air stream drying of fermented feed unit mahine, rate of drying is fast, needs 10～12 seconds, the material temperature rise is little, can effectively keep the thalline biological activity, can keep color and luster and fragrance well, guarantee the quality of feed.
(C) nucleic acid.
(c) 1, cytoclasis.The isolated yeast of whizzer is washed, adds water with clear water in yeast, with the pH value 4.5 of acid with mixture, feed 45～55 ℃ of hot water thermal insulatings, yeast autolysis 24 hours, in autolysing yeast, add 0.20% prozyme, carrying out the yeast cell fragmentation through nanometer ram machine, is 97.9 through check cytoclasis filter, gets the yeast miscellany;
(c) 2, lixiviate.Above-mentioned yeast mixt is put into container, add the extracting solution of 54～58 ℃ of preheatings, (10% cetyl trimethylammonium bromide＜CTAB〉0.14Mol/L naci solution), centrifugal in 54～58 ℃ of stirrings 30 minutes, get supernatant liquor.Repeat said process;
(C) 3, alcohol precipitation drying.The supernatant liquor that merges above-mentioned extracted twice adds ethanol and makes and contain the alcohol amount and reach 70%, the collecting precipitation thing, and drying promptly gets nucleic acid dry powder (DNA, RNA);
(D) amino acid
(D) 1, size mixing.With 1: 2 ratio furnishing slurries, pH value was adjusted into 6.3～6.9, heats 60～62 ℃, 30 minutes with (C) throw out;
(D) 2, hydrolysis.Add proteolytic enzyme, α-Dian Fenmei, beta-amylase, stirring is mixed, stirs once, and is hydrolyzed under the 40-50 temperature, till molecular weight is less than 3000 dalton in 4 hours; Till;
(D) 3, press filtration.Hydrolyzed solution is carried out press filtration under 120 order filter clothes, filtrate enters concentrating pan, and to be evaporated to solid content be 20～30%, and vapor pressure is the 1-2 kg/cm, obtains compound amino acid primary liquid;
(D) 4, powder process.With the aminoacids solution of concentrated solution with the spray-dryer drying of dusting, amino acid dry powder.
(E) fermented wine (gin)
1, (E) 1, fermentation: the raw material of handling well is gone into jar, and the ratio of raw material and water is 100: 270～330, and temperature is at 28～30 degree, bent ratio is 10～13%, and the ratio of distiller's yeast is 5～7%, goes into a jar fermentation simultaneously, want the open type fermentation to discharge carbon dioxide packing, oxygen intake during fermentation.Leavening temperature does not surpass 32 degree, can open the rake heat radiation.Change low temperature fermentation over to after 3-4 (pol is 3.5%) day, temperature is about 12～15 degree.Get new wine after 27～30 days.
(E) 2, arrangement: new wine is accelerated the ripening with high-frequency electronic concussion instrument, the method that adopts ozone oxidation, wine to combine then with complex type molecular sieve of macromolecular material etc., remove in the wine, materials such as potato spirit, ethyl oleate, ethyl linoleate, ethyl palmitate, filter then new wine.
(E) 3, urge old.Adopt the drinks catalytic aging technology of Tianjin Nankai University.This technical matters flow process is as follows:
New wine → wine pump → preheater → ager → water cooler → ripe wine.Use this technology that following advantage is arranged:
(1) can effectively remove new vinosity, make the wine sweet coordination that becomes refreshing only, neither change former wine style, not have back anti-phenomenon again, its effect of accelerating the ripening is quite stored sth. in a cellar the nature storage.(2) can effectively remove the assorted flavor of heresy in the wine, reduce the lead content in the wine, improve the quality of wine.This technology network address
Http:// nku.nankai＞edu.cn. days Tianjin Nankai Wei Jin road 94Number, phone: 022-23508838
Embodiment: get the grain after the 100kg process shells (skin), takes off embryo, cleaning, pulverize the back gelatinization, entering the filling of fermenting, temperature adds distiller's yeast at 28 °, add αDian Fenmei after 3 hours and carry out proteolysis, add the fermentation of three primary yeasts after 1.5 hours, discharge carbonic acid gas during fermentation, need oxygen, adopt stirring or logical oxygen to make fermented liquid seethe cooling when temperature is high, sugared content entered low temperature fermentation (secondary fermentation) at 3.5% o'clock, and this moment, temperature was at 11～14 ℃, discharge carbonic acid gas, anaerobically fermenting.27～30 days wine fermentation maturations.
Propose to separate with the yeast of whizzer, make the fermented liquid solid-liquid separation with pulling the frame filter with throw out lower floor.
Yeast carries out nucleic acid extraction earlier, extracts amino acid then, in the remaining goods and materials backspace solid (vinasse).Test detection factually:, 100kg grain produces 300kg fermented liquid, 25kg solid (vinasse); The 300kg fermented liquid produces the 31.68kg dry yeast, and yeast amplifying nucleic acid content is 8～15, and pure nucleic acid powder extraction yield is 5%, and (purity 86%) extracts 1.58kg nucleic acid dry powder; Aminoacids content 30%～45%, extraction yield 13% (purity 98%), 4.16kg amino acid dry powder.
The 1kg fermented liquid produces the 92.572g carbonic acid gas when fermentation, can reclaim the 40.08kg carbonic acid gas, purity 99.99%.
Fermented wine (gin).Test detection factually: 100kg raw material production fermented wine (gin) 300kg, ethanol content 18～20.This wine vivo acid content 0.55g/L.(attached examining report)
Solid (vinasse).Test detection factually: 100kg raw material production solid (vinasse) 25kg.Inner protein 40.3g/100g, vitamin V B1 (VitB1), VB2 (riboflavin), VPP (nicotinic acid), VE.Probiotic bacterium content 8,000,000,000/more than the gram; Multivitamin, digestive ferment, trace element, 18 seed amino acids and multiple biologically active peptides, aminoacids content is as follows: the dry solid of every 1Kg (vinasse) contains each seed amino acid/mg: aspartic acid 4.0mg, Threonine 1.83mg, Serine 2.52mg, L-glutamic acid 9.75mg, proline 3 .34mg, glycine 2.14mg, L-Ala 2.56mg, Gelucystine 1.67mg, Xie Ansuan 2.32mg, methionine(Met) 0.97mg, Isoleucine 1.84mg, leucine 4.68mg, tyrosine 1.55mg, phenylalanine 2.21mg, Methionin 2.04mg, ammonia (NH) 0.71mg, Histidine 1.12mg, arginase 12 .14, ∑ AA45.58mg.
Claims (1)
1, from transforming, the grain fermentation extracts carbonic acid gas, active polypeptide feed, nucleic acid, amino acid, five kinds of products of fermented wine (gin) continuously.Its concrete production method is as follows:
(1), fermentation.
With grain shelling (skin), take off embryo, cleaning, pulverize the back gelatinization, go into fermentation and irritate, add song (liquor song) and add αDian Fenmei after 3 hours and carry out proteolysis, add three primary yeasts after 1.5 hours, fermentation.Temperature entered secondary fermentation afterwards in 4 days at 28～32 ℃, and temperature is controlled at 11～14 degree, gets new wine after 30 days.
(2) separate.
Centrifuge dewatering is used in the yeast extraction of fermented liquid bottom, with pulling the frame filter with the fermented liquid solid-liquid separation.
(3) extract.
(A), extract carbonic acid gas.The carbon dioxide recovery equipment of installing is irritated in fermentation.
(B), active polypeptide feed.With isolating solid (vinasse), be adjusted to the standard of reaching according to feed formulation, dry, granulation.
(C), nucleic acid extraction.
(C) 1, cytoclasis.With the isolated yeast usefulness of whizzer, enzymolysis carries out the yeast cell fragmentation through nanometer ram machine, gets the yeast miscellany;
(C) 2, lixiviate.Above-mentioned yeast mixt is put into container, centrifugal, get supernatant liquor.Repeat to get supernatant liquor;
(C) 3, alcohol precipitation drying.Above-mentioned extracted twice is got supernatant liquor, and adding ethanol makes and contains pure collecting precipitation thing, and drying promptly gets nucleic acid (DNA, RNA);
(D) amino acid extracts.
(D) 1, size mixing.With 1: 2 ratio furnishing slurries, pH value was adjusted into 6.3～6.9, heats 60～62 ℃, 30 minutes with (C) throw out;
(D) 2, hydrolysis.Add proteolytic enzyme, α-Dian Fenmei, beta-amylase is hydrolyzed under the 40-50 temperature;
(D) 3, press filtration.Hydrolyzed solution is carried out press filtration under 120 order filter clothes, filtrate enters concentrating pan and is evaporated to solid, obtains compound amino acid primary liquid;
(D) 4, powder process.With the aminoacids solution of concentrated solution with the spray-dryer drying of dusting, amino acid dry powder.(E) fermented wine (gin).
(E) 1, fermentation, grain is handled, and adds song and adds yeast, go into to irritate fermentation, after 17～30 days new wine.
(E) 2, arrangement, remove the bad goods and materials in the new wine.
(E) 3, urge oldly, make the molecule of new wine stable, reduce the dry peppery of new wine, it is soft to make it mouthfeel.
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| CNA2006100713818A CN1858184A (en) | 2006-03-24 | 2006-03-24 | Extracting carbon dioxide, polypeptide feed, nucleic acid, amino acid and fermented wine from grain fermentation |
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| CNA2006100713818A CN1858184A (en) | 2006-03-24 | 2006-03-24 | Extracting carbon dioxide, polypeptide feed, nucleic acid, amino acid and fermented wine from grain fermentation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719920A (en) * | 2015-04-09 | 2015-06-24 | 广州超亚化妆品科技有限公司 | Polypeptide amino acid mixture and its preparation method and applications |
| CN106661081A (en) * | 2014-04-21 | 2017-05-10 | 莱科威尔营养有限公司 | Biomass extracts and methods thereof |
-
2006
- 2006-03-24 CN CNA2006100713818A patent/CN1858184A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106661081A (en) * | 2014-04-21 | 2017-05-10 | 莱科威尔营养有限公司 | Biomass extracts and methods thereof |
| CN104719920A (en) * | 2015-04-09 | 2015-06-24 | 广州超亚化妆品科技有限公司 | Polypeptide amino acid mixture and its preparation method and applications |
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