CN1854294A - Escherichia expression system of secretory expression recombinant human epidermal growth factor - Google Patents
Escherichia expression system of secretory expression recombinant human epidermal growth factor Download PDFInfo
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- CN1854294A CN1854294A CNA2005100252893A CN200510025289A CN1854294A CN 1854294 A CN1854294 A CN 1854294A CN A2005100252893 A CNA2005100252893 A CN A2005100252893A CN 200510025289 A CN200510025289 A CN 200510025289A CN 1854294 A CN1854294 A CN 1854294A
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Abstract
Description
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Claims (9)
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CNB2005100252893A CN100489096C (en) | 2005-04-21 | 2005-04-21 | Escherichia expression system for secreting and expressing recombinant human epidermal growth factor |
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CNB2005100252893A CN100489096C (en) | 2005-04-21 | 2005-04-21 | Escherichia expression system for secreting and expressing recombinant human epidermal growth factor |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102212596A (en) * | 2010-04-01 | 2011-10-12 | 孙民富 | Preparation method of human epidermal growth factor |
CN102952817A (en) * | 2011-08-16 | 2013-03-06 | 上海昊海生物科技股份有限公司 | Method for production of recombinant human epidermal growth factor by temperature induction |
CN103060249A (en) * | 2012-06-06 | 2013-04-24 | 浙江普洛康裕制药有限公司 | Escherichia coli and method for efficiently secreting and expressing human epidermal growth factor by using same |
CN103834676A (en) * | 2014-02-28 | 2014-06-04 | 中国科学院福建物质结构研究所 | Plasmid vector of escherichia coli secretory expression heterologous protein and establishment method of plasmid vector |
CN107987150A (en) * | 2017-12-19 | 2018-05-04 | 江苏艾迪药业有限公司 | A kind of method that hEGF can be prepared in the slave urine of industrialized production |
CN110564661A (en) * | 2019-09-23 | 2019-12-13 | 杭州纽龙生物科技有限公司 | Method for culturing engineering bacteria for expressing recombinant human epidermal growth factor |
CN112175063A (en) * | 2020-10-28 | 2021-01-05 | 宁波博睿瀚达生物科技有限公司 | Process for preparing high-purity recombinant epidermal growth factor by high performance liquid chromatography |
CN112209999A (en) * | 2020-10-28 | 2021-01-12 | 宁波博睿瀚达生物科技有限公司 | Method for rapidly separating pigment in recombinant epidermal growth factor fermentation liquid |
CN116555310A (en) * | 2023-03-28 | 2023-08-08 | 广州汉方合成生物研究中心(有限合伙) | Method for high-throughput screening of heterologous constitutive promoter and application thereof |
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2005
- 2005-04-21 CN CNB2005100252893A patent/CN100489096C/en active Active
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102212596A (en) * | 2010-04-01 | 2011-10-12 | 孙民富 | Preparation method of human epidermal growth factor |
CN102952817A (en) * | 2011-08-16 | 2013-03-06 | 上海昊海生物科技股份有限公司 | Method for production of recombinant human epidermal growth factor by temperature induction |
CN102952817B (en) * | 2011-08-16 | 2014-07-09 | 上海昊海生物科技股份有限公司 | Method for production of recombinant human epidermal growth factor by temperature induction |
CN103060249A (en) * | 2012-06-06 | 2013-04-24 | 浙江普洛康裕制药有限公司 | Escherichia coli and method for efficiently secreting and expressing human epidermal growth factor by using same |
CN103834676A (en) * | 2014-02-28 | 2014-06-04 | 中国科学院福建物质结构研究所 | Plasmid vector of escherichia coli secretory expression heterologous protein and establishment method of plasmid vector |
CN107987150A (en) * | 2017-12-19 | 2018-05-04 | 江苏艾迪药业有限公司 | A kind of method that hEGF can be prepared in the slave urine of industrialized production |
CN110564661A (en) * | 2019-09-23 | 2019-12-13 | 杭州纽龙生物科技有限公司 | Method for culturing engineering bacteria for expressing recombinant human epidermal growth factor |
CN112175063A (en) * | 2020-10-28 | 2021-01-05 | 宁波博睿瀚达生物科技有限公司 | Process for preparing high-purity recombinant epidermal growth factor by high performance liquid chromatography |
CN112209999A (en) * | 2020-10-28 | 2021-01-12 | 宁波博睿瀚达生物科技有限公司 | Method for rapidly separating pigment in recombinant epidermal growth factor fermentation liquid |
CN116555310A (en) * | 2023-03-28 | 2023-08-08 | 广州汉方合成生物研究中心(有限合伙) | Method for high-throughput screening of heterologous constitutive promoter and application thereof |
CN116555310B (en) * | 2023-03-28 | 2024-03-29 | 广州市金因源生物技术有限公司 | Method for high-throughput screening of heterologous constitutive promoter and application thereof |
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