CN1846711A - Balanced solution with trace elements for extracorporeal culture of bezoar and its prepn process - Google Patents
Balanced solution with trace elements for extracorporeal culture of bezoar and its prepn process Download PDFInfo
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- CN1846711A CN1846711A CNA2006100167927A CN200610016792A CN1846711A CN 1846711 A CN1846711 A CN 1846711A CN A2006100167927 A CNA2006100167927 A CN A2006100167927A CN 200610016792 A CN200610016792 A CN 200610016792A CN 1846711 A CN1846711 A CN 1846711A
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Abstract
The present invention provides balanced solution of trace elements for extracorporeal culture of bezoar and its preparation process. The balanced solution of trace elements is prepared with water soluble compounds sodium selenite, cobalt sulfate, copper sulfate, zinc gluconate, iron citrate, manganese sulfate, magnesium sulfate, sodium chromate and sodium vanadate in balanced proportion. It is added into effective bezoar components to reach the effects of activating various enzymes and immunocytes in human body, strengthening the metabolism of tissues and cell network, enhancing bioactive function of bezoar and raising the medicine effect of bezoar.
Description
Technical field
The present invention relates to a kind of preparation method of In vitro cultured Calculus Bovis effective ingredient mixed liquor, specifically, is to disclose to add trace element solution and preparation method thereof in a kind of In vitro cultured Calculus Bovis preparation process, belongs to Chinese medicine feedstock production technical field.
Background technology
Calculus Bovis involved in the present invention is famous and precious Chinese medicine material, and high medical value is arranged.At present, domestic have 650 kinds of Chinese patent medicines that contain the Calculus Bovis composition, adds to 42 kinds of Chinese patent medicines again and ban use of the artificial Calculus Bovis.The natural Calculus Bovis demand is big thus, and existing output far can not satisfy the demand of China's national medicine industrial development.
In vitro cultured Calculus Bovis has been included " state-promulgated pharmacopoeia " ministerial standard in 2005 in, mainly is with compositions such as bilirubin, taurine, deoxycholic acid, cholesterol, and settles the standard.The In vitro cultured Calculus Bovis preparation process only is added with a small amount of several trace element, so that facilitate the formation of Calculus Bovis calculus.Nearly 21 kinds of natural Calculus Bovis trace element kinds, some is very little, and height has very big difference again with the producing region, and how uneven, and general trace element total content only accounts for Calculus Bovis 3.53---6.05 gram.
Modern study shows that trace element has high biological activity and catalysis biochemical reaction, when human body must trace element and aminoacid, protein or other organic unity close, form various enzymes, hormone, vitamin, be ferrum, copper, zinc, manganese and selenic hundreds of enzyme, contain cobaltic vitamin B12, ferruginous cytochrome oxidases etc. can make metal-organic double compound produce various biological actions, the biochemical reaction of special physiological function and height.
Few and uneven again according to In vitro cultured Calculus Bovis trace element kind; in order to improve the medicinal biological activity of In vitro cultured Calculus Bovis; in the In vitro cultured Calculus Bovis forming process, add trace element balance (biological element) content proportioning aqueous solution; make its various trace element kinds comprehensive; the content balance reaches for restraining to 8.5-10.5 the trace element total content to increase Calculus Bovis medicine biopotency.
Summary of the invention
The invention provides a kind of In vitro cultured Calculus Bovis trace element balanced contents proportioning water soluble compound that is used for, purpose improve In vitro cultured Calculus Bovis medicinal organism activity exactly.
The invention also discloses In vitro cultured Calculus Bovis and add the purposes that trace element solution is used to prepare the various enzymes of human activin and promotes tissue network metabolic drug.
The invention provides a kind of In vitro cultured Calculus Bovis and add trace element solution, is to be that feedstock production forms by water soluble compound, and the percentage by weight of each component raw material is:
Sodium selenite 0.1-0.2 gram, cobaltous sulfate 0.4-0.6 gram, copper sulfate 1-4 gram, zinc gluconate 6-10 gram, ferric citrate 5-10 gram, manganese sulfate 0.5-0.8 gram, magnesium sulfate 0.1-0.3 gram, sodium chromate 0.1-0.2 gram, vanadic acid sodium 0.005-0.010 gram, surplus is a water for injection.
The preferred weight percent of each component raw material of water soluble compound is:
Sodium selenite 0.2 gram, cobaltous sulfate 0.6 gram, copper sulfate 1 gram, zinc gluconate 10 grams, ferric citrate 10 grams, manganese sulfate 0.5 gram, magnesium sulfate 0.3 gram, sodium chromate 0.1 gram, vanadic acid sodium 0.005 gram, surplus is a water for injection.
Make In vitro cultured Calculus Bovis have index controlled, accounting for the Calculus Bovis total content is 8.5-10.5%.
Concrete steps of the present invention are:
1, take by weighing raw material by required weight:
2, add injection water 50-60ml in step 1, heated and boiled stirs, fully dissolving, and cooling adds the injection water again to 100ml, filters promptly.
3, with trace element in the step 2, water soluble compound adds to 1000 milliliters in In vitro cultured Calculus Bovis effective ingredient mixture, transfers PH5.4-5.6 with lactic acid, and heating evaporation moisture is put coating pan system shape, drying, quality inspection, packing.
The related In vitro cultured Calculus Bovis effective ingredient mixture of step 3 is meant that the applicant declares " natural calculus bovis in vitro breeding method " (number of patent application: 200510046122.5) the in vitro cultivation natural Calculus Bovis of patent of invention.
The present invention is a Rare Elements Preparations, increases selenic content, is relevant with the health of cardiovascular and cerebrovascular vessel because of selenium, and it can protect the capillary vascular health, prevents that permeability from oozing out the generation of the exudative diathesis disease of formation.Add the In vitro cultured Calculus Bovis preparation process, strengthen medicine aspect raising human body self active immunity power, significant effect is arranged.But, make the cellular-restoring health of each organ of body owing to the balance metabolism of trace element.
By to animal injection balance trace element, carry out pharmacodynamic experiment, the present invention of clinical trial proof adds trace element to improving the Calculus Bovis biological activity remarkable result.
Pharmacodynamics test
The present invention has done a large amount of experiments various animal rabbits, dog, cat, pig, cattle, sheep, proves safely, has no side effect.
The animal sheep mixes poisoning with Rogor and decis by experiment, causes gastrointestinal hemorrhage regulating liver-QI, nephrotoxicity degeneration matched group, half death, through treating with trace element intramuscular injection experimental group, after the treatment, symptom takes a turn for the better, 3 routine serious symptom sheep injections began to occur appetite after 3 days, recovered normal after April, blood examination erythrocyte 1,500,000 to 1100 about ten thousand, cut open two liver fat change disappearance extremely, become aubergine by yellow, size, it is normal that quality is recovered, and illustrates that medicine of the present invention has the effect of repairing impaired organ.
Clinical trial
1, the clinical experiment of micro-element injection lung large-area fibreization that human immune deficiency is caused obtains very well results.Lung large-area fibre clinical case 1 example, through hormone therapy 1 year, clinical symptoms alleviated a little, but weak, hypoimmunity, 3 times sb.'s illness took a favorable turn for the injection of trace element injection muscles, and pulmonary fibrosis disappearance 70 grams are injected and are not had flu later six months after 5 months.
2, to cancer postoperative patient 2 examples, especially digestive tract recovers extreme difference, and the amount of having a meal is few, and flatulence is not relaxed and diarrhoea after meal, weight loss, and intramuscular injection twice, appetite increases, and flatulence and diarrhoea are not lacked flu, and body constitution increases.
3, hemorrhage to cerebrovascular sclerosis, renal anemia, the erythrocyte aplastic anemia, gastritis, diseases such as cholecystitis is 2-3 injection of intramuscular injection in 6-9 month, and body constitution strengthens, and clinical symptoms subtracts, and is improving aspect the gastrointestinal function, and effective percentage about 90 restrains.
4, to 14 routine diabetes patients' application, the body constitution of generally becoming thin is recovered, and energy is good, and blood sugar recovery is normal, weight increase, effective percentage 95 grams.
5, to weak and sickly, infantile anorexia and dysplasia, body constitution strengthens after using intramuscular injection, has rosy cheeks, and appetite is good, has no interest.Body constitution is recovered to reach more than 90 grams.
6, to coronary heart disease core ischemia 3 examples, by injecting 3 times, improve blood supply of cardiac muscle, make damaged myocardium recover clinical symptom disappearance, physical recovery health.
Toxicity test
Trace element injection circulation to animal a large amount of experimental results show that safety, have no side effect, human body 60 examples (wherein aged and adult's 53 examples, child's 7 examples) are also very safe, have no adverse reaction.In view of micro-element injection at animal and human's body effect, just balance trace element (biological element) aqueous solution chemical compound is used for In vitro cultured Calculus Bovis preparation technology, to improve the bioactive medical value of Calculus Bovis.
The detection method of trace element
1, copper: precision is measured this product 1ml, put in the 100ml volumetric flask, add hydrochloric acid solution (1 → 100) and be diluted to scale, shake up, (" 2000 editions each methods of appendix VD. of Chinese pharmacopoeia are injected atomic spectrophotometer, measure absorbance at 324.8nm wavelength place according to the atom spectrophotography, according to the content of the copper of standard curve calculation sample, its scope 400ug/ml-1600ug/ml).
2, ferrum: precision is measured this product 1ml, put in the 200ml volumetric flask, add hydrochloric acid solution (1 → 100) and be diluted to scale, shake up, (" 2000 editions each methods of appendix VD. of Chinese pharmacopoeia are injected atomic spectrophotometer, measure absorbance at 248.3nm wavelength place according to the atom spectrophotography, according to the content of the copper of standard curve calculation sample, its scope 1143ug/ml-2286ug/ml).
3, zinc: precision is measured this product 1ml, put in the 100ml volumetric flask, add hydrochloric acid solution (1 → 100) and be diluted to scale, shake up, (" 2000 editions each methods of appendix VD. of Chinese pharmacopoeia are injected atomic spectrophotometer, measure absorbance at 213.9nm wavelength place according to the atom spectrophotography, according to the content of the copper of standard curve calculation sample, its scope 855ug/ml-1425ug/ml).
4, cobalt: precision is measured this product 1ml, put in the 50ml volumetric flask, add hydrochloric acid solution (1 → 100) and be diluted to scale, shake up, (" 2000 editions each methods of appendix VD. of Chinese pharmacopoeia are injected atomic spectrophotometer, measure absorbance at 250.7nm wavelength place according to the atom spectrophotography, according to the content of the copper of standard curve calculation sample, its scope 152ug/ml-228ug/ml).
Adopt spectrophotography, cannot measure the coprime scope of other yuan at different wavelength.
The specific embodiment:
By following examples the present invention is described for example further, and do not limit the present invention in any way, under the prerequisite that does not deviate from technical solution of the present invention, any change or change that those of ordinary skills that the present invention did are realized easily all will fall within the claim scope of the present invention.
Embodiment 1
Get sodium selenite 20g, cobaltous sulfate 60g, copper sulfate 200.0g, zinc gluconate 1000g, ferric citrate 1000g, manganese sulfate 50g, magnesium sulfate 30g, sodium chromate 10g, vanadic acid sodium 1g; benzyl alcohol 200ml; add injection water 30-40 gram earlier; heated and boiled, abundant stirring and dissolving is after the cooling; add benzyl alcohol; add In vitro cultured Calculus Bovis mixed liquor 100,000 ml again, transfer PH5.4-5.8 heating evaporation moisture, coating pan system shape, drying with lactic acid.
Embodiment 2
Get sodium selenite 10g, cobaltous sulfate 40g, copper sulfate 100.0g, zinc gluconate 600g, ferric citrate 500g, manganese sulfate 60g, magnesium sulfate 15g, sodium chromate 15g, vanadic acid sodium 0.5g.Compound method is with embodiment 1.
Embodiment 3
Get sodium selenite 15g, cobaltous sulfate 50g, copper sulfate 400.0g, zinc gluconate 800g, ferric citrate 700g, manganese sulfate 70g, magnesium sulfate 20g, sodium chromate 15g, vanadic acid sodium 0.7g, compound method is with embodiment 1.
Claims (4)
1, a kind of In vitro cultured Calculus Bovis adds trace element solution; be to be made by the percentage by weight of following each component raw material: sodium selenite 0.6-0.2 gram, cobaltous sulfate 0.4-0.6 gram, copper sulfate 1-4 gram, zinc gluconate 6-10 gram, ferric citrate 5-10 gram, manganese sulfate 0.5-0.8 gram, magnesium sulfate 0.1-0.3 gram, sodium chromate 0.1-0.2 gram, vanadic acid sodium 0.005-0.010 gram, surplus is a water for injection.
2, according to the described trace element solution of claim 1, be to be made by the percentage by weight of following each component raw material: sodium selenite 0.2 gram, cobaltous sulfate 0.6 gram, copper sulfate 1 gram, zinc gluconate 10 grams, ferric citrate 10 grams, manganese sulfate 0.5 gram, magnesium sulfate 0.3 gram, sodium chromate 0.1 gram, vanadic acid sodium 0.005 gram, surplus is a water for injection.
3, add the preparation method of trace element solution in a kind of In vitro cultured Calculus Bovis preparation process, may further comprise the steps:
(1) takes by weighing following raw material in proportion; Sodium selenite 0.6-0.2 gram, cobaltous sulfate 0.4-0.6 gram, copper sulfate 1-4 gram, zinc gluconate 6-10 gram, ferric citrate 5-10 gram, manganese sulfate 0.5-0.8 gram, magnesium sulfate 0.1-0.3 gram, sodium chromate 0.1-0.2 gram, vanadic acid sodium 0.005-0.010 gram;
(2) add injection water 50-60ml in step 1, heated and boiled stirs, fully dissolving, and cooling adds the injection water again to 100ml, filters promptly;
(3) trace element water-soluble chemical compound in the step 2 is added to 1000 milliliters in In vitro cultured Calculus Bovis effective ingredient mixture, transfer PH6.4-6.6 heating evaporation moisture content, put coating pan system shape, drying, quality inspection, packing with lactic acid.
4, a kind of In vitro cultured Calculus Bovis adds the purposes that trace element solution is used to prepare various enzymes of human activin and promotion tissue network metabolic drug.
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| CNA2006100167927A CN1846711A (en) | 2006-04-25 | 2006-04-25 | Balanced solution with trace elements for extracorporeal culture of bezoar and its prepn process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302511A (en) * | 2010-06-24 | 2012-01-04 | 攀枝花兴辰钒钛有限公司 | Application of medicinal composition to preparation of medicine for treating organic sexual dysfunction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302511A (en) * | 2010-06-24 | 2012-01-04 | 攀枝花兴辰钒钛有限公司 | Application of medicinal composition to preparation of medicine for treating organic sexual dysfunction |
| CN102302511B (en) * | 2010-06-24 | 2015-06-03 | 攀枝花兴辰钒钛有限公司 | Application of medicinal composition to preparation of medicine for treating organic sexual dysfunction |
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