CN1844404A - Process for producing hybrid long chain dicarboxylic acid by microorganism fermentation - Google Patents
Process for producing hybrid long chain dicarboxylic acid by microorganism fermentation Download PDFInfo
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- CN1844404A CN1844404A CN 200610038331 CN200610038331A CN1844404A CN 1844404 A CN1844404 A CN 1844404A CN 200610038331 CN200610038331 CN 200610038331 CN 200610038331 A CN200610038331 A CN 200610038331A CN 1844404 A CN1844404 A CN 1844404A
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- dicarboxylic acid
- long chain
- chain dicarboxylic
- hybrid long
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Abstract
The invention discloses a method of microbiological fermentation to product mixing long-chain diacid, the method contain the procedure of mixing bacterial, shake flask bacterial, first bacterial pot culture, second bacterial pot culture, fermentation, demulsification and stratification, decolouration, crystallization, press filtration, drying and so on. The invention use a kind of technique, a kind of bacterial to product mixing long-chain diacid, break-through the condition of only use single bacterial to product single variety diacid; simple produce technique, easy-to-operate, the mainly raw material, light wax oil, can be put into operation directly and dispose fractionation, decrease investment, save cost.
Description
Technical field:
The present invention relates to a kind of production method of hybrid long chain dicarboxylic acid.
Background technology:
China takes the lead in having invented the technology of utilizing microorganism petroleum fermentation production SL-AH in the world, and industrialization, and has developed the technology of utilizing the microorganism petroleum fermentation to produce tridecanyldicarboxylic acid, ten four-carbon dicarboxylic acids.But the equal manufacture order component of these technologies long-chain biatomic acid, and used main raw material light wax oil needs through rectifying.
Summary of the invention:
The object of the present invention is to provide and a kind ofly utilize a kind of bacterial classification to produce hybrid long chain dicarboxylic acid, and technology is simple, the method for easy-operating producing hybrid long chain dicarboxylic acid by microorganism fermentation.
Technical solution of the present invention is:
A kind of method of producing hybrid long chain dicarboxylic acid by microorganism fermentation is characterized in that: comprise the following steps:
(1) bacterial classification mixes: select the candida tropicalis bacterial strain, and carry out the cultivation in 1～3 generation;
(2) shake-flask seed: malt extract medium under 26～32 ℃ of conditions, was cultivated on rotary shaker 40～48 hours, cultivated kind of a liquid;
(3) first class seed pot is cultivated: will plant liquid and put into first class seed pot, inoculation under 26～29 ℃, 300～360rpm stirring velocity, was cultivated 35～42 hours;
(4) the secondary seed jar is cultivated: will put into the secondary seed jar through the bacterium liquid that first class seed pot is cultivated, and inoculate, and under 25～30 ℃, 200～280rpm stirring velocity, cultivate 40～50 hours;
(5) fermentation: the bacterium liquid that adds light wax oil and cultivate through the secondary seed jar in fermentor tank, under 25～30 ℃ of conditions, stir fermentation 150～200 hours with 150～210 rev/mins rotating speeds;
(6) breakdown of emulsion layering: after fermentation is finished, carry out the breakdown of emulsion bisque, and be depressed into the layering jar, place layering 1～3 day, the upper strata is residual hydrocarbon, and lower floor is the bacterium layer, and the middle level clear liquid is a hybrid long chain dicarboxylic acid, emits the upper strata, collects the middle level.
(7) hybrid long chain dicarboxylic acid that collection is obtained decolour, crystallization, press filtration, dry product.
During step (2) shake-flask seed, shaking table rotation rotating speed is 200～250 rev/mins.In the step (3), (4) ventilation be 1: 0.6～0.8.Air flow is 1: 0.6～0.8 in the step (5), and pressure is 0.7～0.8 normal atmosphere.When handling, decolouring adopts the gac processing of decolouring.Light wax oil adopts the 200# light wax oil in the step (5).
The present invention produces hybrid long chain dicarboxylic acid with a kind of technology, a kind of bacterial classification, has broken through the present situation that can only utilize single culture manufacture order kind diprotic acid; Production technique is simple, and is easy to operate, and used main raw material light wax oil (as 200#) can directly use, and needn't first rectification process, reduced investment, saved cost.
The invention will be further described below in conjunction with embodiment.
Embodiment:
A kind of method of producing hybrid long chain dicarboxylic acid by microorganism fermentation comprises the following steps:
(1) bacterial classification mixes: select the candida tropicalis bacterial strain, and carry out the cultivation in 1～3 generation;
(2) shake-flask seed: with 3000ml triangular flask preparation 10Be ' malt extract medium, and with it under 26～32 ℃ of (26 ℃, 29 ℃, 32 ℃ of examples) conditions, on rotary shaker, cultivate 40～48 hours (example 40,45,48 hours), cultivate kind of a liquid with 200～250 rev/mins of (200 rev/mins, 220 rev/mins, 250 rev/mins of examples) rotating speeds;
(3) first class seed pot is cultivated: will plant liquid and put into first class seed pot, inoculation is at 400L liquid amount, 2.4L inoculum size, 1: 0.6～0.8 (example 1: 0.6,1: 0.7,1: 0.8) ventilation, tank pressure 1kg/m
3, under 26～29 ℃ (26 ℃, 27 ℃, 29 ℃ of examples), 300～360rpm (routine 300rpm, 330rpm, the 360rpm) stirring velocity, cultivate 35～42 hours (example 35,39,42 hours);
(4) the secondary seed jar is cultivated: will put into the secondary seed jar through the bacterium liquid that first class seed pot is cultivated, inoculation is at 3.5 tons of liquid amounts, inoculum size 400L, 1: 0.6～0.8 (example 1: 0.6,1: 0.7,1: 0.8) ventilation, tank pressure 1kg/m
3, under 25～30 ℃ (25 ℃, 27 ℃, 30 ℃ of examples), the 200～280rpm stirring velocity, cultivate 40～50 hours (example 40 hours, 45 hours, 50 hours);
(5) fermentation: the bacterium liquid that in fermentor tank, adds the 200# light wax oil and cultivate through the secondary seed jar, 25 ℃ of 25～30 ℃ of examples, 27 ℃, 30 ℃), air flow is 1: 0.6～0.8 (example 1: 0.6,1: 0.7,1: 0.8), pressure is under 0.7～0.8 atmospheric pressure, ferments 150～200 hours with the rotating speed stirring of 150～210 rev/mins (150 rev/mins, 180 rev/mins, 210 rev/mins of examples);
(6) breakdown of emulsion layering: after fermentation is finished, carry out the breakdown of emulsion bisque, and be depressed into the layering jar, place layering 1～3 day, the upper strata is residual hydrocarbon, and lower floor is the bacterium layer, and the middle level clear liquid is a hybrid long chain dicarboxylic acid, emits the upper strata, collects the middle level.
(7) hybrid long chain dicarboxylic acid that collection obtained adopt gac decolour processing, crystallization, with pressure filter to the press filtration of crystalline product, Tumble Dry product.
Claims (6)
1, a kind of method of producing hybrid long chain dicarboxylic acid by microorganism fermentation is characterized in that: comprise the following steps:
(1) bacterial classification mixes: select the candida tropicalis bacterial strain, and carry out the cultivation in 1～3 generation;
(2) shake-flask seed: malt extract medium under 26～32 ℃ of conditions, was cultivated on rotary shaker 40～48 hours, cultivated kind of a liquid;
(3) first class seed pot is cultivated: will plant liquid and put into first class seed pot, inoculation under 26～29 ℃, 300～360rpm stirring velocity, was cultivated 35～42 hours;
(4) the secondary seed jar is cultivated: will put into the secondary seed jar through the bacterium liquid that first class seed pot is cultivated, and inoculate, and under 25～30 ℃, 200～280rpm stirring velocity, cultivate 40～50 hours;
(5) fermentation: the bacterium liquid that adds light wax oil and cultivate through the secondary seed jar in fermentor tank, under 25～30 ℃ of conditions, stir fermentation 150～200 hours with 150～210 rev/mins rotating speeds;
(6) breakdown of emulsion layering: after fermentation is finished, carry out the breakdown of emulsion bisque, and be depressed into the layering jar, place layering 1～3 day, the upper strata is residual hydrocarbon, and lower floor is the bacterium layer, and the middle level clear liquid is a hybrid long chain dicarboxylic acid, emits the upper strata, collects the middle level.
(7) hybrid long chain dicarboxylic acid that collection is obtained decolour, crystallization, press filtration, dry product.
2, the method for producing hybrid long chain dicarboxylic acid by microorganism fermentation according to claim 1 is characterized in that: during step (2) shake-flask seed, shaking table rotation rotating speed is 200～250 rev/mins.
3, the method for producing hybrid long chain dicarboxylic acid by microorganism fermentation according to claim 1 and 2 is characterized in that: in the step (3), (4) ventilation be 1: 0.6～0.8.
4, the method for producing hybrid long chain dicarboxylic acid by microorganism fermentation according to claim 1 and 2 is characterized in that: air flow is 1: 0.6～0.8 in the step (5), and pressure is 0.7～0.8 normal atmosphere.
5, the method for producing hybrid long chain dicarboxylic acid by microorganism fermentation according to claim 1 and 2 is characterized in that: adopt the gac processing of decolouring when decolouring is handled.
6, the method for producing hybrid long chain dicarboxylic acid by microorganism fermentation according to claim 1 and 2 is characterized in that: light wax oil adopts the 200# light wax oil in the step (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200610038331 CN1844404A (en) | 2006-02-14 | 2006-02-14 | Process for producing hybrid long chain dicarboxylic acid by microorganism fermentation |
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| CN 200610038331 CN1844404A (en) | 2006-02-14 | 2006-02-14 | Process for producing hybrid long chain dicarboxylic acid by microorganism fermentation |
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| CN 200610038331 Pending CN1844404A (en) | 2006-02-14 | 2006-02-14 | Process for producing hybrid long chain dicarboxylic acid by microorganism fermentation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985416A (en) * | 2010-04-30 | 2011-03-16 | 山东瀚霖生物技术有限公司 | Refining technology for producing long carbon chain dicarboxylic acid by using biological fermentation process |
| CN111039776A (en) * | 2019-12-31 | 2020-04-21 | 淄博广通化工有限责任公司 | High-purity mixed long carbon chain dicarboxylic acid and preparation method thereof |
-
2006
- 2006-02-14 CN CN 200610038331 patent/CN1844404A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985416A (en) * | 2010-04-30 | 2011-03-16 | 山东瀚霖生物技术有限公司 | Refining technology for producing long carbon chain dicarboxylic acid by using biological fermentation process |
| CN101985416B (en) * | 2010-04-30 | 2016-11-09 | 上海凯赛生物技术研发中心有限公司 | Biological fermentation process produces the process for refining of long carbochain biatomic acid |
| CN111039776A (en) * | 2019-12-31 | 2020-04-21 | 淄博广通化工有限责任公司 | High-purity mixed long carbon chain dicarboxylic acid and preparation method thereof |
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