CN1837370A - Method for alcohol fermentation by immobilization of yeast cell - Google Patents
Method for alcohol fermentation by immobilization of yeast cell Download PDFInfo
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- CN1837370A CN1837370A CNA2005100418884A CN200510041888A CN1837370A CN 1837370 A CN1837370 A CN 1837370A CN A2005100418884 A CNA2005100418884 A CN A2005100418884A CN 200510041888 A CN200510041888 A CN 200510041888A CN 1837370 A CN1837370 A CN 1837370A
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- fixed yeast
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses an alcohol ferment production method of fixed yeast cell, which comprises the following steps: adding 10 mL 10 percent yeast suspension liquid in the 90 mL 9 percent polyvinyl alcohol composite gel solution; stirring evenly; pouring the solution in the culture dish to pave; disposing 24-48 h at -20--30 deg.c; cutting the mould into block shape with 12mm*10mm*12mm appearance; breeding in the 12 degreeBe' wort culture medium within 40 percent wort and 60 percent crop mash for 20-48 h at 20-32 deg.c with 100 r per min; adding the fixed yeast in the 16 Bx crop sugar liquid (pH 4.5-4.8) according to 4-8 percent to proceed interval or half-continuous alcohol ferment. The inveiton displays stable operation and high mechanic strength without poisonous and side effect, which simplifies the preparation and reduces the cost.
Description
Technical field:
The present invention relates to a kind of production method of utilizing fixed yeast cell to carry out zymamsis.A kind of specifically is the production method that the fixed yeast cell of carrier carries out zymamsis with the polyvinyl alcohol plural gel.
Background technology:
Immobilized cell is that the cell with biologically active is limited in and still can keeps its distinctive biological nature (as breeding and growth metabolism) in certain space, and a reusable new and high technology.
Yeast cell is fixed in the carrier, behind multiplication culture, is used for zymamsis and produces.Compare with traditional free cell zymamsis and to have: characteristics such as the yeast cell growth reproduction speed is fast, cell count is many, anti-assorted bacterium ability is strong, fermentation efficiency height, and can accelerate fermenting speed greatly, shorten fermentation period, effectively improve plant factor, improve alcohol output and reduce cost, can obtain the economic benefit bigger than traditional technology.
In recent years, periodical literature and patent gazette have announced that the multiple polyvinyl alcohol (PVA) that utilizes carries out the immobilization of microorganism cells.Announced a kind of polyvinyl alcohol immobilized enzyme/method of microorganism as Chinese patent CN1302863, be that polyvinyl alcohol, sodium alginate, acrylamide are mixed with water, make polyvinyl alcohol solution, enzyme/microorganism is carried out immobilization, wherein acrylamide is as linking agent.Chinese patent CN1076488 has announced the preparation method of polyvinyl alcohol microbe or enzyme immobilization carrier, be with after microorganism or the mixing of enzyme PVA solution, place saturated boric acid solution, make it form spheroid in short period of time, contact with phosphate solution with being about to this spheroid, it is fully hardened, and make microorganism or enzyme immobilization carrier.Though aforesaid method can obtain intensity immobilized cell preferably, in actually operating, exist preparation section complexity, energy consumption and reach cost more greatly than problems such as height.
Summary of the invention:
The object of the invention provides a kind of production method of utilizing fixed yeast cell to carry out zymamsis.
The present invention includes the following step: 10 milliliter 10% yeast suspension is joined in 90 milliliter 9% the polyvinyl alcohol plural gel solution, pour into after stirring and be paved into flat board in the culture dish, then-20 ℃～-30 ℃ following freezing treatment 24～48 hours, take out after the moulding and be cut into bulk, physical dimension is: 12mm * 10mm * 12mm; Multiplication culture 20～48 hours in the malt extract medium of 12 degree Beaume (° Be ') then, malt extract medium are 40% wort and 60% corn mash, and culture temperature is 27 ℃～32 ℃, and shaking speed is 100r/min; After treating that propagation is good, fixed yeast is joined by 6～8% carrier addition in the hydrol (initial pH4.5～4.8) of 16 Bo Likesi (Bx) and carry out fixed yeast intermittent type or semicontinuous zymamsis.
The time of fixed yeast multiplication culture in malt extract medium is 36 hours, and culture temperature is 30 ℃ ± 1.The carrier addition of fixed yeast is 7%.
The carrier of immobilized cell of the present invention is to be the main raw material synthetic with polyvinyl alcohol, borax, MALEIC ANHYDRIDE and glycerol, wherein used polyvinyl alcohol is the high molecular weight water soluble polymer that is formed through polymerization, alcoholization by Vinyl Acetate Monomer, its basicity is more than 95%, the polymerization degree 1750 ± 50.If the polymerization degree is crossed low then colloid instability, easy-formation not, too high then viscosity raises, and causes the carrier porosity to reduce, and influences the embedding effect.
Characteristics such as it is good that the made fixed yeast of the present invention has chemical stability, and the physical strength height is nontoxic, have no side effect, and better biocompatibility is arranged, and preparation is simple, and is with low cost.It is strong that more traditional yeast zymamsis has anti-assorted bacterium ability, advantages such as fermentation efficiency height.
Description of drawings:
Fig. 1 is a process flow sheet of the present invention.
Embodiment
The following examples can make those skilled in the art understand the present invention better, but do not limit the present invention in any way.
Embodiment 1:
The synthetic method of the carrier of immobilized cell---polyvinyl alcohol (PVA) plural gel (was applied for a patent in 2004, application number: 2004100388085) as follows, 9 gram polyvinyl alcohol slowly are dissolved in 100 milliliters 85 ℃～95 ℃ the hot water, the limit edged stirs, after treating that polyvinyl alcohol dissolves fully, the glycerol that adds 4 milliliters, stir, when constantly stirring, add the borax (with 1: 1 hot water dissolving) that has dissolved 1 good gram then, the limit edged stirs, carry out gelation and handle, crosslinking time is 2～15 minutes, adds the MALEIC ANHYDRIDE (with 1: 1 hot water dissolving) of 1 gram then lentamente, constantly stir, till the no cotton-shaped coagulum, gained is transparent in gelating soln, slightly milky thickness colloidal sol is polyvinyl alcohol plural gel.
The yeast suspension of 10mL10% is joined in the PVA plural gel solution of 90mL9%, pour into after stirring and be paved into flat board in the culture dish, then at-20 ℃ of following freezing treatment 48h, take out after the moulding and be cut into bulk, physical dimension is: 12mm * 10mm * 12mm, then in the malt extract medium of 12b degree Beaume (° Be ') shaking table (rotating speed: 100r/min) cultivate 36h, malt extract medium is 40% wort and 60% corn mash, and culture temperature is 30 ℃ ± 1.After treating that propagation is good, fixed yeast is joined by 7% carrier addition in the liquid glucose (initial pH value 4.5～4.8) of 16 Bo Likesi (Bx) and carry out the zymamsis of fixed yeast intermittent type.The result shows: propagation back yeast count can reach 1.2 * 10
9Individual/g, the zymamsis productive rate reaches 12%, and support strength is better, and anti-assorted bacterium ability is strong, and fermentation period 27～33h can reuse more than the 200d continuously.
The comparative example A:
Repeat embodiment 1, following difference is arranged: the yeast suspension of 10mL10% is joined in 90mL8%, 10%, 11%, 12% the PVA plural gel solution.Measure under the similarity condition in embodiment 1, the result shows: the hyperplasia yeast cells number reaches 1 * 10
9Individual/g, 9.2 * 10
8Individual/g, 8.9 * 10
8Individual/g, 8.8 * 10
8Individual/g, the zymamsis productive rate is respectively: 11.3%, 11%, 9.8%, 9.7%.
Comparative Examples B:
Repeat embodiment 1, following difference is arranged: fixed yeast is joined by 3%, 5%, 9% and 12% carrier addition in the liquid glucose (initial pH value 4.5～4.8) of 16Bx and carry out the zymamsis of fixed yeast intermittent type.Measure under the same condition in embodiment 1, the result shows: the zymamsis productive rate is respectively: 6.3%, 10.6%, 11.1%, 10.3%.
Comparative Examples C:
Repeat embodiment 1, following difference is arranged: 10mL8%, 12%, 15% and 20% yeast suspension are joined in the PVA plural gel solution of 90mL9%.The result shows: propagation back yeast count can reach 0.96 * 10
9Individual/g, 1.1 * 10
9Individual/g, 0.89 * 10
9Individual/g, 0.84 * 10
9Individual/g, the zymamsis productive rate is respectively: 11.2%, 10.9%, 9.8%, 9.1%.
Embodiment 2:
The yeast suspension of 10mL10% is joined in the PVA plural gel solution of 90mL9%, pour into after stirring and be paved into flat board in the culture dish, behind-20 ℃ of following freezing treatment 24h, be cut into the fritter of 12mm * 10mm * 12mm, shaking table (rotating speed: 100r/min) cultivate 36h in the malt extract medium of 12 degree Beaume (° Be ') then, malt extract medium is 40% wort and 60% corn mash, and culture temperature is 30 ℃ ± 1.After treating that propagation is good, fixed yeast is joined by 7% carrier addition in the liquid glucose (initial pH value 4.5～4.8) of 16 Bo Likesi (Bx) and carry out the zymamsis of fixed yeast intermittent type.The result shows: propagation back yeast count can reach 0.96 * 10
9Individual/g, zymamsis productive rate 11.4% is reused 200d continuously.This may be to cause the aperture of carrier and air strike rate less owing to freezing time is less, and then has influence on the rate of propagation of yeast cell in carrier, and effect is not as embodiment 1.
By the comparison of the embodiment of the invention and Comparative Examples, illustrate that the made fixed yeast product of the present invention has mechanical property preferably, prepare characteristics such as simple, with low cost.It is strong that more traditional yeast zymamsis has anti-assorted bacterium ability, characteristics such as fermentation efficiency height.Can improve plant factor, alcohol output effectively and reduce cost.
Claims (3)
1. production method that fixed yeast cell carries out zymamsis, it comprises the following steps:
10 milliliter 10% yeast suspension joined in 90 milliliter 9% the polyvinyl alcohol plural gel solution, pour into after stirring and be paved into flat board in the culture dish, then-20 ℃～-30 ℃ following freezing treatment 24～48 hours, take out after the moulding and be cut into bulk, physical dimension is 12mm * 10mm * 12mm; Multiplication culture 20～48 hours in the malt extract medium of 12 degree Beaume (° Be ') then, malt extract medium are 40% wort and 60% corn mash, and culture temperature is 27 ℃～32 ℃, and shaking speed is 100r/min; After treating that propagation is good, fixed yeast is joined by 6～8% carrier addition in the hydrol (initial pH4.5～4.8) of 16 Bo Likesi (Bx) and carry out fixed yeast intermittent type or semi continuous zymamsis.
2. according to the process of claim 1 wherein that the time of fixed yeast multiplication culture in malt extract medium is 36 hours, culture temperature is 30 ℃ ± 1.
3. according to the method for claim 1 or 2, wherein the carrier addition of fixed yeast is 7%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100418884A CN1837370A (en) | 2005-03-25 | 2005-03-25 | Method for alcohol fermentation by immobilization of yeast cell |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100418884A CN1837370A (en) | 2005-03-25 | 2005-03-25 | Method for alcohol fermentation by immobilization of yeast cell |
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| CN1837370A true CN1837370A (en) | 2006-09-27 |
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| CNA2005100418884A Pending CN1837370A (en) | 2005-03-25 | 2005-03-25 | Method for alcohol fermentation by immobilization of yeast cell |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101644702B (en) * | 2009-09-02 | 2014-07-23 | 清华大学 | Method for rapid BOD determination using Saccharomyces cerevisiae as biometric elements |
| CN109593627A (en) * | 2019-02-21 | 2019-04-09 | 江苏润知农业技术服务有限公司 | A kind of electric field-enhanced brewage process of sea-buckthorn Chinese wolfberry health-care fruit wine |
| CN109943555A (en) * | 2018-12-29 | 2019-06-28 | 南京工业大学 | Method for continuously preparing yeast wine by utilizing surface immobilization technology to perform alcohol fermentation |
-
2005
- 2005-03-25 CN CNA2005100418884A patent/CN1837370A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101644702B (en) * | 2009-09-02 | 2014-07-23 | 清华大学 | Method for rapid BOD determination using Saccharomyces cerevisiae as biometric elements |
| CN109943555A (en) * | 2018-12-29 | 2019-06-28 | 南京工业大学 | Method for continuously preparing yeast wine by utilizing surface immobilization technology to perform alcohol fermentation |
| CN109593627A (en) * | 2019-02-21 | 2019-04-09 | 江苏润知农业技术服务有限公司 | A kind of electric field-enhanced brewage process of sea-buckthorn Chinese wolfberry health-care fruit wine |
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