CN1834629A - Kit for discriminating breewing vinegar and preparing vinegar - Google Patents
Kit for discriminating breewing vinegar and preparing vinegar Download PDFInfo
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- CN1834629A CN1834629A CN 200610013463 CN200610013463A CN1834629A CN 1834629 A CN1834629 A CN 1834629A CN 200610013463 CN200610013463 CN 200610013463 CN 200610013463 A CN200610013463 A CN 200610013463A CN 1834629 A CN1834629 A CN 1834629A
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Abstract
This invention discloses a kit that can distinguish brewed vinegar and blended vinegar. It includes box, there are standard color plate, color comparison tube, trickle irrigator, reagent bottle A, B and C. Reagent in A is acidic potassium permanganate aqueous solution of mass percent concentration 1-3%. PH value of the acidic permanganate aqueous solution is 1.5-4.0. Reagent in B is sodium bisulphate aqueous solution of concentration is 5-10%. Reagent in C is chromotropic acid sulfuric acid aqueous solution of concentration is 2-5%, the concentration of sulfuric acid aqueous solution is 0.02-0.05 mol/L. all kinds of vinegars in commodities circulation fields and vinegar generating enterprise can be quickly and accurately detected by this method. Its operation is simple, cost is low, and unprofessional man can also use it to make spot detecting.
Description
Technical field
The invention belongs to the food inspection technical field, relate to a kind of kit of differentiating making vinegar and preparation of vinegar.
Background technology
Vinegar is one of requisite flavouring of people's daily life.Making vinegar is meant and is used alone or as a mixture materials such as various starch-containing, sugar, the liquid tart flavour flavouring that forms through the microbial fermentation brew.Wherein contain multiple nutritional components such as alcohol, aldehyde, ester, reducing sugar, free amino acid, comprise liquid fermentation edible vinegar and solid state fermentation vinegar two big classes.Preparation of vinegar is meant that with the flavouring edible vinegar that mixed preparing such as edible ice acetic acid, food additives form, it is not with health role based on making vinegar (account for volume 50%).But, quite a few preparation of vinegar is arranged in the market, adopt glacial acetic acid (very the person uses industrial glacial acetic acid) illegally to blend into vinegar and peddle at a low price; And some so-called " making vinegars " also blend with " preparation of vinegar " and form, and wherein do not have the composition of making vinegar at all, and national relevant market product access system (QS authentication) has been violated in these behaviors.If edible these preparation of vinegar inferior of long term consumer, residual free mineral acid, methyl alcohol and heavy metal etc. become branch that human body vitals such as liver and kidney etc. are produced huge harm in the industry glacial acetic acid, and this will seriously encroach on consumers in general's legitimate rights and interests.Related content according to vinegar act.std GB18187-2000 " making vinegar ", GB2719-2003 " vinegar hygienic standard " and SB10337-2000 " preparation of vinegar " etc., whether the sanitary index that only can differentiate edible vinegar is qualified, but often is difficult to become the standard of brewageing with preparation of vinegar of differentiating.So, set up a kind of scene that can be used for and differentiate the method for brewageing with preparation of vinegar fast, have very big social demand and scientific meaning.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, provide a kind of and can make things convenient for the discriminating making vinegar of use and the kit of preparation of vinegar at the scene.
Technical scheme of the present invention is summarized as follows:
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube, dropper, the A reagent bottle, B reagent bottle and C reagent bottle, reagent in the described A reagent bottle is that mass percent concentration is 1%～3% acid potassium permanganate aqueous solution, the pH of described acid potassium permanganate aqueous solution is 1.5～4.0, reagent in the described B reagent bottle is that mass percent concentration is 5%～10% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 2%～5% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.02～0.5mol/L.
Acid in the described acid potassium permanganate aqueous solution is that phosphoric acid also can be hydrochloric acid, and the pH of described acid potassium permanganate aqueous solution is 2.5～3.0.
The mass percent concentration of described aqueous solution of sodium bisulfite preferably 7%～8%.
A kind of kit of differentiating making vinegar and preparation of vinegar of the present invention can carry out all kinds of vinegars of commodity circulation or vinegar manufacturing enterprise fast, sensitive, check exactly.Kit of the present invention has easy and simple to handle, and use cost is low, and is easy to carry, and the layman also can operate and can carry out the on-the-spot characteristics that detect.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 2% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is phosphoric acid, the pH of described acid potassium permanganate aqueous solution is 2.5, reagent in the described B reagent bottle is that mass percent concentration is 7% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 4% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.1mol/L.
The method of differentiating making vinegar and preparation of vinegar is:
From box body, take out color comparison tube, drawing vinegar 2.5ml to be measured adds in the color comparison tube, drip the reagent in the 0.5mlA reagent bottle then, behind the reaction 2min, drip the reagent in the 0.5ml B reagent bottle again, reagent after shaking up in the C reagent bottle of dropping 1.0ml, (15-35 ℃) reaction 3-5min under the room temperature, observe its change color, the contrast ratio colour table is differentiated the essential composition of vinegar, and the person is that (purple is dark more for making vinegar if reaction solution becomes the darkviolet, the composition that makes vinegar is many more), then be preparation of vinegar if it becomes colorless.Get the making vinegar of high-quality (as Shanxi mature vinegar etc.) simultaneously through after the dilution repeatedly,, verify the technical indicators (the results are shown in Table 1.) such as detection limit, accuracy of this method with the experiment that compares of gas chromatography (GC) method of routine.
The comparison of table 1. the present invention and conventional gas chromatography (GC) method
| Detectability | Accuracy | On-the-spot detectability | The pre-treatment requirement | Testing cost | The technical ability requirement | Analysis speed | |
| GC method this method | Brewage composition＞2% and brewage composition＞5% | Better good | Differ from | Very strictness is lower | Height is very cheap | Height is very low | 4-6h/ sample 5-10min/ sample |
If when the vinegar of being differentiated during use has a large amount of burnt sugar coloring, then need measure again through distillation decoloration.The present invention is a kind of to differentiate reagent in the kit of making vinegar and preparation of vinegar, can preserve under room temperature, lucifuge condition 6-8 month.
A kind of principle of work of differentiating the kit of making vinegar and preparation of vinegar of the present invention: this detection kit is earlier by the strongly acidic solution oxidation of potassium permanganate according to the endemic element in the making vinegar (as ethanol, acetaldehyde, reducing sugar etc.), again after solution of sodium bisulfite decolouring reduction, react with specificity developer-chromotropic acid sulfuric acid solution, and produce one and have the principle of the violet complex of specific absorption wavelength (λ max=665nm), and preparation of vinegar does not show any color in that the effect of developer is next.Utilize visual colorimetry and by standard colorimetric plate, can go out to brewage and preparation of vinegar by on-the-spot directly qualitative identification.
Embodiment 2
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 1% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is phosphoric acid, the pH of described acid potassium permanganate aqueous solution is 1.5, reagent in the described B reagent bottle is that mass percent concentration is 8% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 3% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.02mol/L.
Embodiment 3
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 3% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is phosphoric acid, the pH of described acid potassium permanganate aqueous solution is 3, reagent in the described B reagent bottle is that mass percent concentration is 5% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 2% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.5mol/L.
Embodiment 4
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 2% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is phosphoric acid, the pH of described acid potassium permanganate aqueous solution is 4, reagent in the described B reagent bottle is that mass percent concentration is 6% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 5% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.1mol/L.
Embodiment 5
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 1% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is hydrochloric acid, the pH of described acid potassium permanganate aqueous solution is 3.0, reagent in the described B reagent bottle is that mass percent concentration is 9% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 4% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.3mol/L.
Embodiment 6
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 3% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is hydrochloric acid, the pH of described acid potassium permanganate aqueous solution is 1.5, reagent in the described B reagent bottle is that mass percent concentration is 10% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 3% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.4mol/L.
Embodiment 7
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 3% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is hydrochloric acid, the pH of described acid potassium permanganate aqueous solution is 4.0, reagent in the described B reagent bottle is that mass percent concentration is 5% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 2% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.5mol/L.
Embodiment 8
A kind of kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube (10ml), plastic dropper (1.0ml), the A reagent bottle, B reagent bottle and C reagent bottle and kit operation brief introduction, reagent in the described A reagent bottle is that mass percent concentration is 3% acid potassium permanganate aqueous solution, acid in the described acid potassium permanganate aqueous solution is hydrochloric acid, the pH of described acid potassium permanganate aqueous solution is 2.5, reagent in the described B reagent bottle is that mass percent concentration is 5% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 2% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.3mol/L.
Embodiment 9
To containing the more coloured vinegar of caramel colorant, behind the distillation rear decoloring, from box body, take out color comparison tube, the vinegar sample 2.5ml to be measured that draws decolouring adds in the color comparison tube, drip the reagent in the 0.5mlA reagent bottle then, behind the reaction 2min, drip the reagent in the 0.5mlB reagent bottle again, the reagent after shaking up in the C reagent bottle of dropping 1.0ml, (15-35 ℃) reaction 4min under the room temperature, observe its change color, the contrast ratio colour table is differentiated the essential composition of vinegar, and the person is that (purple is dark more for making vinegar if reaction solution becomes the darkviolet, the composition that makes vinegar is many more), then be preparation of vinegar if it becomes colorless.Get high-quality make vinegar (as Shanxi mature vinegar etc.) simultaneously and do control experiment.
Embodiment 10
Chosen the synthetic vinegar sample in common in the market light-coloured vinegar, rice vinegar, mature vinegar and laboratory, adopted a kind of kit of differentiating making vinegar and preparation of vinegar of the present invention respectively, with the GC method of routine (when wherein adopting kit of the present invention to differentiate, it differentiates that step is identical with the above embodiments 1), above-mentioned several samples are all carried out replication 7 times, and the testing result of the two has been done relatively (seeing Table 2.).
Table 2. uses kit of the present invention and conventional sense method to vinegar sample detection result (n=7)
| The sample title | This method measured value (%) | Conventional GC method detected value (%) | Result's the situation that meets |
| Light-coloured vinegar rice vinegar mature vinegar laboratory synthetic vinegar | 25 40 64 10 | 22.8 40 69 8.8 | Fine good better fine |
Claims (4)
1. kit of differentiating making vinegar and preparation of vinegar, comprise box body, be provided with standard colorimetric plate in the described box body, color comparison tube, dropper, the A reagent bottle, B reagent bottle and C reagent bottle, reagent in the described A reagent bottle is that mass percent concentration is 1%～3% acid potassium permanganate aqueous solution, the pH of described acid potassium permanganate aqueous solution is 1.5～4.0, reagent in the described B reagent bottle is that mass percent concentration is 5%～10% aqueous solution of sodium bisulfite, reagent in the described C reagent bottle is that mass percent concentration is the aqueous sulfuric acid of 2%～5% chromotropic acid, and the concentration of described aqueous sulfuric acid is 0.02～0.5mol/L.
2. a kind of kit of differentiating making vinegar and preparation of vinegar according to claim 1 is characterized in that the acid in the described acid potassium permanganate aqueous solution is phosphoric acid, and the pH of described acid potassium permanganate aqueous solution is 2.5～3.0.
3. a kind of kit of differentiating making vinegar and preparation of vinegar according to claim 1 is characterized in that the acid in the described acid potassium permanganate aqueous solution is hydrochloric acid, and the pH of described acid potassium permanganate aqueous solution is 2.5～3.0.
4. a kind of kit of differentiating making vinegar and preparation of vinegar according to claim 1, the mass percent concentration that it is characterized in that described aqueous solution of sodium bisulfite is 7%～8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100134637A CN100439901C (en) | 2006-04-14 | 2006-04-14 | Kit for discriminating breewing vinegar and preparing vinegar |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100134637A CN100439901C (en) | 2006-04-14 | 2006-04-14 | Kit for discriminating breewing vinegar and preparing vinegar |
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| Publication Number | Publication Date |
|---|---|
| CN1834629A true CN1834629A (en) | 2006-09-20 |
| CN100439901C CN100439901C (en) | 2008-12-03 |
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| CNB2006100134637A Expired - Fee Related CN100439901C (en) | 2006-04-14 | 2006-04-14 | Kit for discriminating breewing vinegar and preparing vinegar |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102890084A (en) * | 2011-07-18 | 2013-01-23 | 山西省生物研究所 | Rapid identifying kit for Shanxi mature vinegar |
| CN111912840A (en) * | 2020-07-15 | 2020-11-10 | 郑州科技学院 | Method for rapidly detecting whether total acid of brewed vinegar is qualified |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100507519C (en) * | 2005-09-13 | 2009-07-01 | 西北农林科技大学 | Method for identifying vinegar types by using spectral curve |
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2006
- 2006-04-14 CN CNB2006100134637A patent/CN100439901C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102890084A (en) * | 2011-07-18 | 2013-01-23 | 山西省生物研究所 | Rapid identifying kit for Shanxi mature vinegar |
| CN111912840A (en) * | 2020-07-15 | 2020-11-10 | 郑州科技学院 | Method for rapidly detecting whether total acid of brewed vinegar is qualified |
| CN111912840B (en) * | 2020-07-15 | 2023-09-22 | 郑州科技学院 | Rapid detection method for whether total acid of brewed vinegar is qualified or not |
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| Publication number | Publication date |
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| CN100439901C (en) | 2008-12-03 |
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Granted publication date: 20081203 Termination date: 20110414 |