CN1824787A - Process for preparing peptone - Google Patents
Process for preparing peptone Download PDFInfo
- Publication number
- CN1824787A CN1824787A CN 200510137997 CN200510137997A CN1824787A CN 1824787 A CN1824787 A CN 1824787A CN 200510137997 CN200510137997 CN 200510137997 CN 200510137997 A CN200510137997 A CN 200510137997A CN 1824787 A CN1824787 A CN 1824787A
- Authority
- CN
- China
- Prior art keywords
- bone
- enzyme
- enzymolysis
- bright
- boiling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention provides a preparation process of peptone. Said invention adopts biological enzymolysis method, and uses fresh bovine bone, dried other bones, animal tissue and entrails as raw material, so that it is low in cost and is applicable to industrial production.
Description
Technical field
The present invention relates to field of biological product, especially a kind of preparation technology of peptone.
Background technology
Every proteinic animal, plant, microorganism and other raw materials of containing is according to range of product and purposes, take physics modes such as high temperature, high pressure or cold soaking, hot dipping and self-dissolving to extract wherein protein, changing into molecular weight through acid, alkali, enzymic hydrolysis is protein fragments mixture about 5000D, is called peptone.Prior art has the method that adopts acid, alkali, enzymic hydrolysis, produce peptone, but these method hydrolysis is not thorough, and quality product is low, the industrial production cost height, and preparation technology's limitation is big.Chinese patent CN1334037A, CN1185960C, CN1046191C disclose the preparation method of polypeptide bone powder, micropeptide bone paste, polypeptide healthful nutriment, mentioned method in the technology with enzymic hydrolysis, but the raw material that it adopted is single or too complicated, raw material is difficult for obtaining, the enzyme of use, and the enzymolysis degree is low, hydrolysis is not thorough, whole complex process, industrial applicibility are not strong, and these open source literatures all do not propose the notion of peptone.
Summary of the invention
The invention provides a kind of preparation technology of peptone, preparation technology's science, reasonable is fit to suitability for industrialized production, selects for use raw material to contain different sorts protein, comprehensive nutrition, and used enzymic hydrolysis is thorough, and cost is low.
Technical solution of the present invention is:
A kind of preparation technology of peptone, raw material is bright ox bone, dried assorted bone and animal tissues, internal organ, wherein bright ox bone consumption 20%-60% does assorted bone consumption 20%-60%, animal tissues, internal organ consumption 10%-30%, processing step is:
A, bright ox bone are broken into the fritter that is not more than 10 * 10cm, isolate gelatine liquid after the boiling;
The back boiling is cleaned in B, dried assorted bone removal of impurities, isolates gelatine liquid;
C, animal tissues, internal organ add 0.1-3 water making beating back homogenate doubly;
D, with the gelatine liquid cooling of A, B step but, the back of deoiling adds the slurries of C step, adjusts temperature to 40 ℃-60 ℃, transfers pH value 8-10, add compound protease and carry out enzymolysis; The conjugated protein enzyme dosage is the 0.2-1% of the contained total protein of said mixture matter, enzymolysis time: 3-5h; The enzyme that goes out that behind the enzymolysis enzymolysis solution heated up, enzyme-removal temperature is 85-100 ℃, goes out 10-20 minute enzyme time; The enzyme that goes out is finished rear decoloring, filtration, concentrates, is drying to obtain.
Remove degrease before the described bright ox bone boiling, water is put into the autoclave boiling after cleaning, and adds water 1-2 doubly, vapor pressure 1.5-4kg, cooking time 2-5h.
Described doing put into the autoclave boiling after assorted bone cleans, and adds 12 times in water, vapor pressure 1.5-4kg, cooking time 2-5h.
Described enzymolysis solution heats up to go out and is cooled to 60-75 ℃ after enzyme is finished, and adds the decolouring of gac and/or alukalin, and consumption is the 1.5-3% of total protein in the raw material, time 30-60 minute.
Enzymolysis solution after the described decolouring filters with plate-and-frame filter press, and filter material cloth is selected the pure cotton canvas of 4 * 4-8 * 8 for use, and pure cotton canvas uses after 0.2%-1% dilute alkaline soln degreasing post rinsing is clean; Filter with microporous membrane is smart again behind the Plate Filtration.
Described drying is vacuum-drying or spraying drying; During spraying drying, the equipment importation hot blast temperature is 150 ℃-200 ℃, and the outlet hot blast temperature is 60 ℃-90 ℃.
Prepare peptone according to the present invention, material choice is utilized local advantageous natural condition, bright ox bone, do animal tissues such as assorted bone, cattle and sheep and internal organ all are easy to obtain, and cheap, and the selection on the technology from the raw materials pretreatment to the compound protease, the method for enzymolysis are all very advanced, be suitable for industrial applications, hydrolysis process is thorough, and prepared using is abundant, peptone product yield height, quality is good, is widely used.
Embodiment
Embodiment one:
Be equipped with 20 kilograms of bright ox bones, do 60 kilograms in assorted bone, 20 kilograms in animal tissues and internal organ.15％
Bright ox bone is sawn into a chainsaw the fritter of 3 * 3cm, clean with clear water after removing degrease, put into autoclave then, the water boiling that adds 20 kilograms, vapor pressure 1.5kg, the glue that cooking time 2h separates wherein is gelatine liquid, contains 3 kilograms in albumen (containing the protein 15 kilogram by per hundred kilograms of bright ox bones calculates) in the gelatine liquid.
The assorted bone of doing of animals such as cattle and sheep is removed impurity, put into autoclave after the cleaning, add 20 kilograms water boiling, vapor pressure 1.5kg, cooking time 2h, the glue that separates wherein is gelatine liquid, contains 9 kilograms in albumen (do assorted bone by per hundred kilograms and contain the calculating of protein 15 kilogram) in the gelatine liquid.
Tissues such as meat, internal organ that the heart, liver, lung, the anorexia of sheep and ox are used rub with industrial mincer, add 2 kilograms water, beat evenly with hollander, contain 2 kilograms in albumen (containing the protein 10 kilogram by per hundred kilograms of animal tissuess and internal organ calculates) in the slurries.
The gelatine liquid cooling but, the back of deoiling mixes the slurries of cattle and sheep animal tissues and internal organ, adjusts temperature to 40 ℃, transfers pH value 8, adds the compound protease (directly buying commodity) that Novozymes Company produces and carry out enzymolysis.Containing total protein in the said mixture matter is 14 kilograms, and the conjugated protein enzyme dosage is 0.028 kilogram.Enzymolysis time: 3h; The enzyme that goes out that behind the enzymolysis enzymolysis solution heated up, enzyme-removal temperature is 85 ℃, goes out 10 minutes enzyme time; Enzymolysis solution heats up to go out and is cooled to 60 ℃ after enzyme is finished, and calculates by total protein to add 0.021 kilogram of decolouring of gac, 30 minutes time.Enzymolysis solution after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 4 * 4 pure cotton canvas for use, and pure cotton canvas uses after 0.2% dilute alkaline soln degreasing post rinsing is clean; Filter with microporous membrane is smart again behind the Plate Filtration.After the smart filter smart filtrate is concentrated, select economic benefits and social benefits falling film type thickner for use.Concentrate the back and carry out spraying drying in the pressure spray dryer tower, the equipment importation hot blast temperature is 150 ℃, and the outlet hot blast temperature is 60 ℃.
After finishing, above-mentioned drying step makes 12.6 kilograms of peptone finished products (yield 90%).
Embodiment two:
Be equipped with 60 kilograms of bright ox bones, do 30 kilograms in assorted bone, 10 kilograms in animal tissues and internal organ.
Bright ox bone is sawn into a chainsaw the fritter of 6 * 6cm, clean with clear water after removing degrease, put into autoclave then, the water boiling that adds 120 kg, vapor pressure 3kg, the glue that cooking time separated wherein in 3 hours is gelatine liquid, contains 9 kilograms in albumen (containing the protein 15 kilogram by per hundred kilograms of bright ox bones calculates) in the gelatine liquid.
After the assorted bone of doing of animals such as cattle and sheep removed impurity, soak, clean then, put into autoclave after the cleaning, 60 kilograms water boiling, vapor pressure 3kg, cooking time 3 hours, the glue glue that separates wherein is gelatine liquid, contains 4.5 kilograms in albumen (do assorted bone by per hundred kilograms and contain the calculating of protein 15 kilogram) in the gelatine liquid.
Tissues such as meat, internal organ that the heart, liver, lung, the anorexia of sheep and ox are used, rub with industrial mincer, add the water of 2 kilograms (2 times), break into homogenate, contain 1 kilogram in albumen (containing the protein 10 kilogram by per hundred kilograms of animal tissuess and internal organ calculates) in the slurries with hollander.
The gelatine liquid cooling but, the back of deoiling mixes the slurries of cattle and sheep animal tissues and internal organ, adjusts temperature to 50 ℃, transfers pH value 9, adds the compound protease (directly buying commodity) that Guangzhou distant sky zymin factory produces and carry out enzymolysis.Containing total protein in the said mixture matter is 14.5 kilograms, and the conjugated protein enzyme dosage is 0.087 kilogram.Enzymolysis time: 4h; The enzyme that goes out that behind the enzymolysis enzymolysis solution heated up, enzyme-removal temperature is 90 ℃, goes out 15 minutes enzyme time; Enzymolysis solution heats up to go out and is cooled to 70 ℃ after enzyme is finished, and calculates by total protein to add 0.145 kilogram of decolouring of gac, 40 minutes time.Enzymolysis solution after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 6 * 6 pure cotton canvas for use, and pure cotton canvas uses after 1% dilute alkaline soln degreasing post rinsing is clean.Filter with microporous membrane is smart again behind the Plate Filtration.After the smart filter smart filtrate is concentrated, select economic benefits and social benefits falling film type thickner for use.Be concentrated in and carry out spraying drying in the pressure spray dryer tower, the equipment importation hot blast temperature is 180 ℃, and the outlet hot blast temperature is 80 ℃.
After finishing, above-mentioned drying step makes 13.8 kilograms of peptone finished products (yield 95%).
Embodiment three:
Be equipped with 50 kilograms of bright ox bones, do 20 kilograms in assorted bone, 30 kilograms in animal tissues and internal organ.
Bright ox bone is sawn into a chainsaw the fritter of 10 * 10cm, clean with clear water after removing degrease, put into autoclave then, add 150 kg of water boilings, vapor pressure 4kg, cooking time 5 hours, the glue that separates wherein is gelatine liquid, contains 7.5 kilograms in albumen (containing the protein 15 kilogram by per hundred kilograms of bright ox bones calculates) in the gelatine liquid.
After the assorted bone of doing of animals such as cattle and sheep removed impurity, soak, clean then, put into autoclave after the cleaning, 60 kilograms water boiling, vapor pressure 4kg, cooking time 5 hours, the glue glue that separates wherein is gelatine liquid, contains 3 kilograms in albumen (do assorted bone by per hundred kilograms and contain the calculating of protein 15 kilogram) in the gelatine liquid.
With tissue, internal organ such as the conscience lung of animals such as cattle and sheep, unedible meat, rub with industrial mincer, add 90 kilograms water, break into homogenate with hollander, contain 3 kilograms in albumen (containing the protein 10 kilogram by per hundred kilograms of animal tissuess and internal organ calculates) in the slurries.
The gelatine liquid cooling but, the back of deoiling mixes the slurries of cattle and sheep animal tissues and internal organ, adjusts temperature to 60 ℃, transfers pH value 10, adds the compound protease (directly buying commodity) that Lingwu, Ningxia safe fortune biochemical product company limited produces and carry out enzymolysis.Containing total protein in the said mixture matter is 13.5 kilograms, and the conjugated protein enzyme dosage is 0.135 kilogram.Enzymolysis time: 5h; The enzyme that goes out that behind the enzymolysis enzymolysis solution heated up, enzyme-removal temperature is 100 ℃, goes out 20 minutes enzyme time; Enzymolysis solution heats up to go out and is cooled to 75 ℃ after enzyme is finished, and calculates by total protein to add 0.405 kilogram of decolouring of gac, 60 minutes time.Enzymolysis solution after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 8 * 8 pure cotton canvas for use, and pure cotton canvas uses after 1% dilute alkaline soln degreasing post rinsing is clean; Filter with microporous membrane is smart again behind the Plate Filtration.After the smart filter smart filtrate is concentrated, select economic benefits and social benefits falling film type thickner for use.Concentrate the back and carry out spraying drying in the pressure spray dryer tower, the equipment importation hot blast temperature is 200 ℃, and the outlet hot blast temperature is 90 ℃.
After finishing, above-mentioned drying step makes 13.2 kilograms of peptone finished products (yield 98%).
Embodiment four:
Other technologies are identical with the foregoing description one to three with condition, and after enzymolysis solution concentrated, drying means adopted vacuum-drying, and plate-and-frame filter press filter material cloth is selected chemical fabric for use during filtration.
Claims (6)
1, a kind of preparation technology of peptone, raw material is bright ox bone, dried assorted bone and animal tissues, internal organ, wherein bright ox bone consumption 20%-60% does assorted bone consumption 20%-60%, animal tissues, internal organ consumption 10%-30%, processing step is:
A, bright ox bone are broken into the fritter that is not more than 10 * 10cm, isolate gelatine liquid after the boiling;
The back boiling is cleaned in B, dried assorted bone removal of impurities, isolates gelatine liquid;
C, animal tissues, internal organ add 0.1-3 water making beating back homogenate doubly;
D, with the gelatine liquid cooling of A, B step but, the back of deoiling adds the slurries of C step, adjusts temperature to 40 ℃-60 ℃, transfers pH value 8-10, add compound protease and carry out enzymolysis; The conjugated protein enzyme dosage is the 0.2-1% of the contained total protein of said mixture matter, enzymolysis time: 3-5h; The enzyme that goes out that behind the enzymolysis enzymolysis solution heated up, enzyme-removal temperature is 85-100 ℃, goes out 10-20 minute enzyme time; The enzyme that goes out is finished rear decoloring, filtration, concentrates, is drying to obtain.
2, remove degrease before the technology as claimed in claim 1, described bright ox bone boiling, water is put into the autoclave boiling after cleaning, and adds 12 times in water, vapor pressure 1.5-4kg, cooking time 2-5h.
3, technology as claimed in claim 1, described doing put into the autoclave boiling after assorted bone cleans, and adds 12 times in water, vapor pressure 1.5-4kg, cooking time 2-5h.
4, technology as claimed in claim 1, described enzymolysis solution heat up to go out and are cooled to 60-75 ℃ after enzyme is finished, and add the decolouring of gac and/or alukalin, and consumption is the 1.5-3% of total protein in the raw material, time 30-60 minute.
5, as claim 1 or 4 described technologies, the enzymolysis solution after the described decolouring filters with plate-and-frame filter press, and filter material cloth is selected the pure cotton canvas of chemical fabric or 4 * 4-8 * 8 for use, and pure cotton canvas uses after 0.2%-1% dilute alkaline soln degreasing post rinsing is clean; Filter with microporous membrane is smart again behind the Plate Filtration.
6, technology as claimed in claim 1, described drying are vacuum-drying or spraying drying; During spraying drying, the equipment importation hot blast temperature is 150 ℃-200 ℃, and the outlet hot blast temperature is 60 ℃-90 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101379976A CN100371454C (en) | 2005-12-26 | 2005-12-26 | Process for preparing peptone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101379976A CN100371454C (en) | 2005-12-26 | 2005-12-26 | Process for preparing peptone |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1824787A true CN1824787A (en) | 2006-08-30 |
| CN100371454C CN100371454C (en) | 2008-02-27 |
Family
ID=36935628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101379976A Expired - Fee Related CN100371454C (en) | 2005-12-26 | 2005-12-26 | Process for preparing peptone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100371454C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669405A (en) * | 2012-05-22 | 2012-09-19 | 新沂市嘉禾鸭业有限公司 | Preparation method of duck bone protein peptide |
| CN102827911A (en) * | 2012-09-24 | 2012-12-19 | 平凉市华科生物技术有限公司 | Preparation method of animal peptone by double-enzyme enzymolysis |
| CN104450840A (en) * | 2014-11-14 | 2015-03-25 | 四川大学 | Production process of bovine bone peptone |
| CN104830937A (en) * | 2015-05-14 | 2015-08-12 | 吉林大学 | Method for preparing peptone by using chicken slaughtering by-products |
| CN105039478A (en) * | 2015-07-06 | 2015-11-11 | 南通凯恒生物科技发展有限公司 | Preparation method of peptone specially used for producing paecilomyces hepiali powder through submerged fermentation |
| CN108753892A (en) * | 2018-06-21 | 2018-11-06 | 吴忠市王国旗生物科技有限公司 | A kind of preparation process of peptone |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1231146C (en) * | 2001-04-08 | 2005-12-14 | 内蒙古草原兴发股份有限公司 | Process for preparing polypeptide bone powder |
| CN1185960C (en) * | 2001-04-08 | 2005-01-26 | 内蒙古草原兴发股份有限公司 | Process for preparing micropeptide bone paste |
| CN1559255A (en) * | 2004-02-19 | 2005-01-05 | 上海交通大学 | Method for producing edible peptone by pressure hydrolyzation of animal protein |
-
2005
- 2005-12-26 CN CNB2005101379976A patent/CN100371454C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669405A (en) * | 2012-05-22 | 2012-09-19 | 新沂市嘉禾鸭业有限公司 | Preparation method of duck bone protein peptide |
| CN102827911A (en) * | 2012-09-24 | 2012-12-19 | 平凉市华科生物技术有限公司 | Preparation method of animal peptone by double-enzyme enzymolysis |
| CN104450840A (en) * | 2014-11-14 | 2015-03-25 | 四川大学 | Production process of bovine bone peptone |
| CN104830937A (en) * | 2015-05-14 | 2015-08-12 | 吉林大学 | Method for preparing peptone by using chicken slaughtering by-products |
| CN105039478A (en) * | 2015-07-06 | 2015-11-11 | 南通凯恒生物科技发展有限公司 | Preparation method of peptone specially used for producing paecilomyces hepiali powder through submerged fermentation |
| CN108753892A (en) * | 2018-06-21 | 2018-11-06 | 吴忠市王国旗生物科技有限公司 | A kind of preparation process of peptone |
| CN108753892B (en) * | 2018-06-21 | 2022-02-01 | 吴忠市王国旗生物科技有限公司 | Preparation process of peptone |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100371454C (en) | 2008-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112062834B (en) | Deep sea fish skin collagen peptide and extraction and preparation method thereof | |
| CN1824787A (en) | Process for preparing peptone | |
| CN101402986B (en) | Method for producing collagen, glutin or collagen by degreasing and deliming of fish scale | |
| CN110655568A (en) | Method for extracting collagen by acid enzymolysis method | |
| CN101744090B (en) | Method for preparing small molecular peptides of soft-shelled turtle | |
| CN108866137A (en) | Fish-skin/collagen peptide preparation method | |
| CN101731305A (en) | Method for manufacturing edible artificial sausage skin from soybean proteins | |
| CN101481723A (en) | Method for preparing collagen peptide by using common carp skin | |
| CN102550824A (en) | Method for producing small peptide amino acid microelement chelate by way of acid hydrolysis of protein | |
| CN1923026A (en) | Preparation of liver peptide | |
| CN113754759A (en) | Process for extracting multiple nutritional ingredients from fish scales | |
| CN104630319B (en) | The method that biological enzyme prepares medical bone gelatin | |
| CN105348383A (en) | Odourless fishskin collagen and preparation method thereof | |
| CN113563459A (en) | Protein extraction process in production process of collagen peptide | |
| CN105385728A (en) | Co-production even-molecular-weight-distribution fibroin-polypeptide and sericin-polypeptide powder and preparing method thereof | |
| CN108753892B (en) | Preparation process of peptone | |
| CN100334225C (en) | I-type protein small peptide and its preparing method | |
| CN101748179A (en) | Preparation method of feather protein peptide | |
| CN104131060B (en) | Corbicula fluminea anti-oxidative peptide and preparation method thereof | |
| CN113150307B (en) | Glycosylated sericin, preparation method and application thereof | |
| CN114605525A (en) | Tuna bone collagen peptide and production method thereof | |
| CN105002246A (en) | Fish polypeptide with uniform molecular weight distribution, and preparation method thereof | |
| CN1712516A (en) | Protein peptone and production thereof | |
| CN112694515A (en) | Deep processing method for extracting soybean protein peptide by membrane method | |
| CN112841569A (en) | Practical nutritional fish scale biological calcium compound chewable tablet and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080227 Termination date: 20100126 |