CN1815215A - Glucose biological sensor capable of eliminating interference - Google Patents
Glucose biological sensor capable of eliminating interference Download PDFInfo
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- CN1815215A CN1815215A CN 200610038603 CN200610038603A CN1815215A CN 1815215 A CN1815215 A CN 1815215A CN 200610038603 CN200610038603 CN 200610038603 CN 200610038603 A CN200610038603 A CN 200610038603A CN 1815215 A CN1815215 A CN 1815215A
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- polypyrrole
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
Present invention discloses interference elimination glucose biosensor referring to new glucose sensor measuring method. It contains double constant potential rectifier, electrolytic bath, and electric current subtracter, wherein said electrolytic bath composed of four electrode system, which arepolypyrrole membrane-glucose oxidase electrode as working electrode W 1, polypyrrole membrane electrode as working electrode W 2, platinum sheet electrode as auxiliary electrode , saturated calomel electrode as reference electrode R. Present invention utilizes polypyrrole-polypyrrole glucose oxidase double working electrode system effectively eliminating variety inorganic substance organic matter coexist body interference to measuring.
Description
Technical field
The present invention relates to a kind of eliminate disturb that glucose sensor measures new method---polypyrrole-polypyrrole glucose oxidase duplex is made the electrode method.
Background technology
Britain scholar Clark and Lyons proposed in 1962, the high degree of specificity of enzymatic reaction and the high sensitivity of electrode response can be combined, and proposed the notion of enzyme electrode thus.From 1967, Updile and Hicks successfully developed the glucolase sensor, constantly had new biology sensor to be developed out to the seventies in 20th century, existing a large amount of bibliographical informations about immobilized enzyme.Enzyme biologic sensor is very helpful to medical diagnosis and environmental analysis etc., as glucose oxidase and uricase sensor.Glucose is one of important products of body metabolism, and numerous disease is relevant with the content of glucose in the blood, measures the concentration of glucose in the human serum, has the important clinical meaning for the diagnosis and the treatment of hypoglycemia, hyperglycaemia and diabetes and other diseases.And the uricase sensor also can provide science foundation accurately for measuring aspects such as cardiovascular disease, ephrosis, tumour.Therefore studying the preparation of enzyme biologic sensor and character had not only had important significance for theories, but also had had tangible practical value.
The report of relevant sensor is a lot, generally is product---the hydrogen peroxide that detects enzymic catalytic reaction under the electromotive force of calibration, the concentration of indirect determination substrate.Because in the sample multiple concurrent is often arranged, these concurrents usually have interference to mensuration.For example, blood of human body is multiple inorganics and organic coexisting body, and when glucose or uric acid content, other material can cause interference to measurement, especially the ascorbic acid in the blood in glucose oxidase electrode or urate oxidase determination of electrode blood.Therefore how weakening effectively and eliminating interference is one of key of exploitation enzyme electrode.
Summary of the invention
The objective of the invention is to invent a kind of glucose biological sensor that can eliminate the impurity interference effectively.
The present invention includes double potentiostat, electrolytic cell, current subtraction device, electrolytic cell is made up of four electrode systems, and polypyrrole film-glucose oxidase electrode is working electrode W1, and the polypyrrole film electrode is working electrode W2, the platinized platinum electrode is auxiliary electrode C, and saturated calomel electrode is contrast electrode R.
The present invention adds identical current potential with accurate double potentiostat on two working electrodes, the electric current difference of two working electrodes is measured by the current subtraction device.Because the polypyrrole film of two electrodes makes under identical condition, when having only chaff interference to exist in the solution, the interference current that chaff interference produces on these two working electrodes is just the same, and therefore, the electric current difference that records is zero.When substrate in the solution and chaff interference coexistence, containing the electric current that the electrode of enzyme records is the response current sum of the response current and the chaff interference of substrates enzymes catalytic reaction, and the electric current that the electrode that does not contain enzyme records is the response current of chaff interference, and the electric current difference that this moment, duplex recorded as electrode is substrates enzymes catalytic reaction electric current.The deficiency that the present invention has avoided prior art to be difficult to eliminate effectively the impurity interference utilizes polypyrrole-polypyrrole glucose oxidase duplex to make electrode system, eliminate the interference to measuring of multiple inorganics and organic coexisting body effectively.
Use the glucose sensor of the present invention's design, can monitor blood sugar, this electrode is safe in utilization, and is nontoxic, and stable performance can repeatedly be used, and can eliminate the interference of other impurity in the blood of human body effectively.
Description of drawings
The structural representation of the glucose oxidase enzyme biologic sensor of four electrode systems that Fig. 1 designs for the present invention.
Fig. 2 is the graph of a relation of four kinds of different solutions response currents and electromotive force.
Below in conjunction with accompanying drawing, introduce the content of glucose oxidase enzyme biologic sensor of four electrode systems of the present invention design in detail.
The present invention is made up of accurate double potentiostat 1, electrolytic cell 2, current subtraction device 3, electrolytic cell 2 is made up of four electrode systems: a polypyrrole film-glucose oxidase electrode (working electrode W1), a polypyrrole film electrode (working electrode W2), a platinized platinum electrode (auxiliary electrode C) and a saturated calomel electrode (contrast electrode R).
Embodiment
As shown in Figure 1, in the glucose oxidase enzyme biologic sensor of four electrode systems that the present invention designs, on two working electrodes, add identical current potential by accurate double potentiostat.The electric current difference of two working electrodes is measured by the current subtraction device.Because the polypyrrole film of two electrodes makes under identical condition, when having only chaff interference to exist in the solution, the interference current that chaff interference produces on these two working electrodes is just the same, and therefore, the electric current difference that records is zero.When substrate in the solution and chaff interference coexistence, containing the electric current that the electrode of enzyme records is the response current sum of the response current and the chaff interference of substrates enzymes catalytic reaction, and the electric current that the electrode that does not contain enzyme records is the response current of chaff interference, and the electric current difference that this moment, duplex recorded as electrode is substrates enzymes catalytic reaction electric current.
Place the relation of measuring response current and electromotive force in the electrolytic cell with following four kinds of solution respectively.
Solution (1) comprises 1.0mmol dm
-3Glucose and 0.1mmol dm
-3The 0.1moldm of ascorbic acid
-3Phosphate buffer solution
Solution (2) comprises 0.1mmol dm
-3The 0.1mol dm of ascorbic acid
-3Phosphate buffer solution
Solution (3) comprises 1.0mmol dm
-3The 0.1mol dm of glucose
-3Phosphate buffer solution
Solution (4) comprises 1.0mmol dm
-3Glucose and 0.1mmol dm
-3The 0.1moldm of ascorbic acid
-3Phosphate buffer solution
The response current of four kinds of buffer solution and the relation of electromotive force have been shown respectively as Fig. 2.
Response current value in the curve 1,2,3 is the three-electrode system mensuration of working electrode by W1, and the response current in the curve 4 is made electrode system by duplex and measured.All data all repeat to survey 3 times at least, and relative error is less than 2%.The difference of the response current of curve 1 and curve 2 is the response current in the corresponding electromotive force lower curve 3, and the response current value of the curve 4 almost response current value with curve 3 is consistent, and promptly curve 4 overlaps substantially with curve 3.Obviously, duplex is done the interference that electrode can be eliminated ascorbic acid effectively.
In order to further specify the antijamming capability of this electrode, the concentration of measuring glucose respectively is 1.0mmol dm
-3, to disturb electroactive material (as: glutathione, 1-halfcystine, paracetamol and uric acid) be to the interference of glucose assays under the normal physiological level conditions.
The result is as shown in the table: under this condition determination, they are effectively eliminated the Recombinant Interferon of the response current of mensuration glucose.Although in actual sample,, can predict that by this experiment the bipolar electrode system can be eliminated these interference because the increase of impurity is disturbed and also may be risen.
Table 1 electrochemical activity chaff interference is to the influence of glucose biological sensor response current
| Chaff interference | Physiological concentration (mmol dm -3) | I G+I/I G |
| Glutathione 1-halfcystine paracetamol uric acid | 0.1 2 0.05 0.5 | 1.000 1.011 1.011 1.016 |
I
G: 1.0mmol dm
-3Response current in the glucose solution, I
G+I: 1.0mmoldm
-3Response current in glucose and the different chaff interference mixed solutions.
Claims (1)
1, a kind of glucose biological sensor that can eliminate interference, comprise double potentiostat, electrolytic cell, current subtraction device, it is characterized in that: electrolytic cell is made up of four electrode systems, polypyrrole film-glucose oxidase electrode is working electrode W1, the polypyrrole film electrode is working electrode W2, the platinized platinum electrode is auxiliary electrode C, and saturated calomel electrode is contrast electrode R.
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| CN 200610038603 CN1815215A (en) | 2006-03-02 | 2006-03-02 | Glucose biological sensor capable of eliminating interference |
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| CN 200610038603 CN1815215A (en) | 2006-03-02 | 2006-03-02 | Glucose biological sensor capable of eliminating interference |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101430303B (en) * | 2007-11-07 | 2012-05-23 | 中国科学院电子学研究所 | Single-layer functional film urease biologic sensor chip and production method thereof |
| CN103940870A (en) * | 2014-04-17 | 2014-07-23 | 佳木斯大学 | Intracellular purine electrochemical-detection method based on enzyme catalysis |
| CN104237336A (en) * | 2014-09-15 | 2014-12-24 | 江苏大学 | Nitrite sensor |
| CN110220956A (en) * | 2019-07-02 | 2019-09-10 | 中国科学院生物物理研究所 | A kind of fructose non-enzymatic electrochemical sensor and its detection method |
| WO2019218562A1 (en) * | 2018-05-17 | 2019-11-21 | 华南理工大学 | Glucose sensor electrode, preparation method therefor and use thereof |
| CN111329490A (en) * | 2018-12-18 | 2020-06-26 | 林少玉 | Non-invasive anti-interference blood glucose detector and method for detecting blood glucose by using same |
-
2006
- 2006-03-02 CN CN 200610038603 patent/CN1815215A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101430303B (en) * | 2007-11-07 | 2012-05-23 | 中国科学院电子学研究所 | Single-layer functional film urease biologic sensor chip and production method thereof |
| CN103940870A (en) * | 2014-04-17 | 2014-07-23 | 佳木斯大学 | Intracellular purine electrochemical-detection method based on enzyme catalysis |
| CN104237336A (en) * | 2014-09-15 | 2014-12-24 | 江苏大学 | Nitrite sensor |
| WO2019218562A1 (en) * | 2018-05-17 | 2019-11-21 | 华南理工大学 | Glucose sensor electrode, preparation method therefor and use thereof |
| US11788984B2 (en) | 2018-05-17 | 2023-10-17 | South China University Of Technology | Glucose sensor electrode, preparation method therefor and use thereof |
| CN111329490A (en) * | 2018-12-18 | 2020-06-26 | 林少玉 | Non-invasive anti-interference blood glucose detector and method for detecting blood glucose by using same |
| CN110220956A (en) * | 2019-07-02 | 2019-09-10 | 中国科学院生物物理研究所 | A kind of fructose non-enzymatic electrochemical sensor and its detection method |
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