CN1800200A - Method for extracting high purity protein from cow milk or soybean waste water - Google Patents
Method for extracting high purity protein from cow milk or soybean waste water Download PDFInfo
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- CN1800200A CN1800200A CN 200510095244 CN200510095244A CN1800200A CN 1800200 A CN1800200 A CN 1800200A CN 200510095244 CN200510095244 CN 200510095244 CN 200510095244 A CN200510095244 A CN 200510095244A CN 1800200 A CN1800200 A CN 1800200A
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- resin
- lactoferrin
- desorbs
- bovinelactoperoxidase
- absorption
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- 108010063045 Lactoferrin Proteins 0.000 claims abstract description 24
- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 claims abstract description 24
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- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 3
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Landscapes
- Dairy Products (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a method for extracting high quality protein form milk or whey water, which adopts the stirring tank with cationic ion-exchange resin to adsorb, desorb and separately extracts the lactoferrin, oxidative enzyme protein and the mixer. It uses film separating technology to do separately condensation and highly purity. The stirring tank can adjust the stirring rotating speed at 20-100rpm; the tank inside wall has swirl-adding flag; the bottom of the tank has resin bed with filter plate; the fast guiding suction tube is positioned on the resin layer upper 8-12cm of the resin bed.
Description
Technical field
The present invention relates to a kind of method of from milk or whey water, extracting high purity protein
Background technology
The present invention relates to a kind ofly from milk or whey water, extract highly purified lactoferrin (bovine lactoferrin simultaneously with stirred pot, be abbreviated as BLF), the method for Bovinelactoperoxidase (bovine lactoperoxidase is abbreviated as BLP) and lactoferrin and Bovinelactoperoxidase mixture.Bovinelactoperoxidase and lactoferrin have the antibiotic of wide spectrum, and be antiviral and improve the immunologic function of body disease-resistant.For example BLP can destroy the tenuigenin of germ, suppresses Yeast Nucleic Acid synthetic in the germ process of growth.Thereby it is to gram-positive microorganism and Gram-negative bacteria, yeast such as Candida albicans, and mould such as black-koji mould all have restraining effect.There is Bovinelactoperoxidase in the secretory product in the mammal, particularly in the milk.But its concentration is very low in first Ruzhong, and its concentration increases sharply and passes to the antibiotic of neonatal as parent after 4-5 days.Because the immune yarn system of neonatal is not physically well developed as yet, need from breast milk, be protected with germ and the virus that resists an invasion.
Bovinelactoferrin (BLF) is a ferruginous glycoprotein, and is opposite with Bovinelactoperoxidase, and it is dense colostrum, and its concentration just descends rapidly after several days.BLF also is a very antibacterial protein of wide spectrum.It can be robbed iron ion from germ, thereby germ mistake iron is suppressed or death.So BLF is to gram-positive microorganism such as streptococcus aureus, suis etc.; Gram-negative bacteria such as intestinal bacteria, dysentery bar mattress, and shiga bacillus; Hp, influenza virus, eczema virus and salivary gland virus all have restraining effect.
Because simple BLP and BLF have the proteic function of biological antibiotic, their two kinds of mixtures together also should have antibiotic, the function of antiviral and enhance immunity power.
The laboratory scale of existing report is isolated BLF, as uses DEAE, SephadexC50, CMSephaseFF, CMToyopearl, BioradMacro-preCM, Dowx-mac-3 and PuriliteC115 ion exchange resin.But these methods are resin to be adorned post use and adopt damping fluid.Such method is inapplicable large-scale industrial practice.Because BLF and the concentration of BLP in milk are very low, produce that commercial economy is worth, highly purified BLP and BLF must adopt the milk of huge amount so that enough BLF and BLP to be provided, such as will produce 20 kilograms of purity daily is 96% above BLF, suppose that its total recovery is 80%, the BLF in the raw material milk is 180mg/L.This just needs 160 tons of milk.And the treatment time of these 160 tons of milk must in 10 hours, finish, not only can increase the chance of milk microbiological contamination because consider long process period, and will set apart to ion exchange resin column regeneration and disinfecting to prepare second day work.Moreover, cause the absorption dynamics of BLF and BLP very slow because milk intermediate ion intensity is about 6-7mS (milli west door), needed 50 minutes just can reach more than 60% of total amount approximately.Therefore must give resin and milk has enough duration of contact.In order to make BLP as much as possible in the milk, BLF contacts the production permutoid reaction with resins exchange functional group simultaneously, must play the linear velocity that guarantees certain milk flowing, and in other words, linear velocity is big more in the post, and its resin absorption dynamics is just fast more.Such as the linear velocity in the post at least will 100cm per hour more than (1.6cm per minute), concerning the garden post that a diameter is 50cm, reach the linear velocity of 1.6cm per minute, its volumetric flow rate just at least will be at 3.2 Liter Per Minutes.If be no less than 50 minutes duration of contact, the length of post will be more than 83cm.But its column length surpasses 50cm when the diameter of post surpasses 30cm, just is difficult to guarantee the quality of its flow and dress post.Thereby above-mentioned these ion exchange resin are for laboratory scale designs, and do not possess the performance that still keeps high flow capacity under the technical scale condition, therefore can't be applied to the production process of scale like this.If moreover in separating the process of telling, adopt damping fluid, also increase production cost greatly, product can't be competed, this is worthless on industrial production especially.
Also have report to adopt ultra-filtration membrane to come from whey, to separate BLF, but the purity of the BLF that this method is produced is very low, only has an appointment about 30%.One of them possible factor is exactly that the molecular weight of BLP and BLF is very close, and the ultrafiltration membrane technique of present stage is impossible with these two kinds of albumen separately.
Have report to extract BLF, but because the iso-electric point of BLF and BLP is very close, this method can't be produced highly purified BLP and BLF with the isoelectric precipitation method.
Have report to use heparin agar affinity chromatography to extract BLF, but the production cost of this method is too high, can't realize technical scale.
Summary of the invention
Can not low cost carry out big industrial problem for solving the existing high purity protein technology of from milk or whey water, extracting.The invention provides a kind of method of from milk or whey water, extracting highly purified lactoferrin, Bovinelactoperoxidase and lactoferrin and Bovinelactoperoxidase mixture with stirred pot simultaneously.
Technical scheme of the present invention is as follows:
A kind of method of from milk or whey water, extracting high purity protein, the stirred pot that its adopt to place Zeo-karb adsorbs, desorbs, lactoferrin, newborn Bovinelactoperoxidase and both mixtures thereof in separation and Extraction milk or the whey liquid, and is aided with membrane separation technique and it is separated concentrates and highly purified.
Stirred pot is adjustable, and the stirring arm rotating speed is 20-100rpm; The jar inner side-wall has the eddy current of increasing baffle plate, and jar end is provided with the resin bed of band screen plate, and 8-12cm highly locates to be provided with quick water conservancy diversion suction filtration pipe above the resin layer on the resin bed.The various transmitter pH meters of controlling for automatically are set in jar, the weight meter, electric conductivity is taken into account thermometer, and the pot liquid holding temperature is 3-160 ℃.Resin is the SPC70 resin, and number of times that resin absorption desorbs and condition can be set according to the requirement of variant production and product purity; The adsorption time of resin is 20-180 minute, and adsorption temp is at 3-30 ℃.The adsorption time of resin is 35-60 minute.Desorbing of resin adopts the two-stage salt concn to desorb lactoferrin, newborn Bovinelactoperoxidase.First step salt concn is 1-2.5%, desorbs newborn Bovinelactoperoxidase, and the time of desorbing is 30-100 minute; Second stage salt concn is 3.5-7%, desorbs lactoferrin, and the time of desorbing is 30-100 minute; The salt concn that desorbs newborn Bovinelactoperoxidase and lactoferrin mixture is to be 30-100 minute the 3-7% time of desorbing; The stir speed (S.S.) that desorbs is 20-100rpm.It is sodium salt, calcium salt that resin desorbs used salt, sylvite; Resin need not regeneration activating after absorption and desorbing technological process, can be for the usefulness of next batch absorption as long as be lower than 1.6ms with reverse osmosis water (RO water) rinsing resin to its ionic strength after desorbing the technology end.Membrane separation technique uses the microfiltration membrane of 0.05-0.1 micron with lactoferrin, newborn Bovinelactoperoxidase separate the attached liquid degerming, remove remaining fat and other small-particle; Ultra-filtration membrane with 1K-30K molecular interception amount carries out desalination and concentrated.Highly purified lactoferrin is that the lactoferrin dope that will desorb desalination and concentration through the absorption of above-mentioned technology is separated the concentrated acquisition that echos ultra-filtration membrane through the absorption of weak-type sun resin such as CM resin again.With adsorb, desorb, the lactoferrin dope of desalination and concentration, newborn Bovinelactoperoxidase dope and lactoferrin thereof, newborn Bovinelactoperoxidase mixing dope carries out vacuum dehydrating at lower temperature or low temperature spray drying becomes powder mixture.
Adopt the form of stirred pot rather than dress post to carry out absorption and separate the process of telling.Thereby need not adorn post and worry the resin column problem of short-circuit.Can increase stirring velocity and separate the kinetics speed of telling to increase absorption.In stirred pot, increase baffle plate, thereby make proteic transmission speed faster.In addition-the more important thing is that installation-suction filtration tube is accelerated drain age velocity on the resin bed surface in jar, in just big industrial production.
Description of drawings
Fig. 1 is a process flow diagram of the present invention
Embodiment
1, adopt the form of stirred pot rather than dress post to carry out absorption and separate the process of telling.Thereby need not adorn post and worry the resin column problem of short-circuit.Can increase stirring velocity and separate the kinetics speed of telling to increase absorption.In stirred pot, increase baffle plate, thereby make proteic transmission speed faster.In addition-the more important thing is that (dotted line among Fig. 1 in the T2 jar) installation-suction filtration tube (FT among Fig. 1 in the T2 jar) is accelerated drain age velocity on the resin bed surface in jar.This is because when absorption and separate when telling to finish, and stirring arm stops stirring.Resin (the thick black line of the level among Fig. 1 in the T2 jar) on the screen plate at the bottom of the action of gravity deposit jar forms resin bed.If at this moment too thin the or too soft liquid that will cause of resin particle is difficult to by resin bed, thereby the flow of discharge opeing diminishes at the bottom of causing jar, and the activity duration is long, and productive rate descends.In order to accelerate drain age velocity, installation-suction filtration pipe on the resin bed surface, make most liquid without resin bed directly through suction filtration pipe and valve V25, V5, VM1 and V6 discharge.When the liquid level in the jar dropped to the resin bed surface, suction filtration pipe FT stopped drawing liquid, remaining liq V5 at the bottom of jar in jar, and VM1 and V6 extract out.Such process is just improved the speed of discharge opeing greatly.
2, this law does not need raw material milk or whey water are regulated pH and ionic strength.And utilize the natural pH and the ionic strength of milk or whey water.Its normal ph should be 6.2-6.9
3, the milk of this paper middle finger is meant heating or the not new fresh milk of thermal sterilization or stale milk and the milk that is mixed with milk powder.
4, this method can directly adopt whole milk and skimmed milk.Or the full-cream or skimmed milk of reduction that is mixed with milk powder.
5, this method can directly be used whey water.
6, Zeo-karb all can be used as the BLF of this method and the absorption resin of BLP, but SPC70 is the first-selected absorption resin of this law.
7, the 20-120 minute absorption time of this paper employing, the absorption time is too short, and yield is low excessively, and the absorption time is long, can improve yield, considers long process period but play.Absorption and separate tell temperature can be at 3-16 degree C.
8, adopt two-stage salt (sodium-chlor) concentration to separate and tell BLP and BLF.First step salt concn is that 1-2.5% separates and tells BLP, and separating the time of telling is 30-100 minute.Second stage salt concn is that 3.5-7% separates and tells BLF, and separating the time of telling is 30-100 minute.
9, this law used salt in separating and telling can be sodium salt such as sodium-chlor etc., can be calcium salt such as calcium chloride etc., also can be sylvite such as Repone K etc.But the first-selected sodium-chlor of this paper.
But 10, this law continuous production, promptly resin does not need other regeneration, and a dust is washed resin to its ionic strength with reverse osmosis water (RO water) and is lower than the absorption that 1.6mS can begin next batch after separating and telling to finish.
11, the stirring velocity in the stirred pot is at 30-70rpm.
12, adopt the microfiltration membrane degerming and the remaining fat of 0.05-0.1 micron, molecule etc.
13, the elutriant of BLP of Shou Jiing and BLP (separate and tell liquid) carries out desalination and concentrated with the ultra-filtration membrane of 1K-30K molecular retention amount.
14, as will producing highly purified BLF, desalination and concentrate good BLF again through the absorption (the T7 jar among Fig. 1) of the positive resin such as the CM resin of a weak-type to remove wherein a small amount of other foreign protein.
15, BLP after concentrating and the mixed solution of BLF, BLP solution and BLF solution can be used the also available spraying drying of freeze-drying drying (FD among Fig. 1) (SD among Fig. 1).Final protein powder packing (the square case among Fig. 1).
16, the leaching salt solution and can use reverse osmosis unit (RO) or electric osmose device for folding recovered brine of ultra-filtration membrane, the RO filtrate can be made water of productive use simultaneously.
T1 among Fig. 1 is milk container or whey water pot.After raw material is transported to stirred pot T2 from the T1 jar, stirred 60-120 minute.After absorption finishes, stop to stir and allowed in 3 minutes after the resin free setting, again by suction filtration villous themeda FT, VM1, V5, V6 is transported to the T3 jar with the milk liquid of absorption.This milk liquid still contains certain density BLP and BLF, so still can be considered as milk or other milk preparation usefulness.
Through the V3 valve with RO water wash resin (full-cream in this way or half fat milk, with the RO water wash of 34 degree C) more than 3 times, to remove milk liquid and the fat of remnants.Leacheate is discharged through valve V4.
(1) produces BLP and BLF mixed protein;
After adding 5-6% salt solution from the V2 valve, stir after 60-100 minute, stop to stir and allowed the resin natural subsidence in 3 minutes, again through FT, V25, V5 and VM5 will separate and tell liquid and be transported to the TMF jar.
To separate the particulate and the fat of telling in the liquid through 0.1 micron microfiltration membrane removes.Mocromembrane filtrate is transported to the T4 jar through VM2 and V8 again.Again from the T4 jar through V18, V15 is by the ultra-filtration membrane desalination and be concentrated to protein concentration more than 6%.Wherein V13 and V8 are the return lines of ultra-filtration membrane, and V7 is the priming valve door.
The concentrated solution of BLPF is transported to the spray-dryer drying or gets the dry powder finished product after V20 is transported to the lyophilizer drying through V21.Powder is light brown green.
Add RO water and be lower than 1.6 milli west doors until ionic strength through the V3 valve drip washing resin that oneself separates after telling at stirred pot T2.Ring waste is discharged from the V4 valve.Stirred pot T2 now waits upon life and prepares the next round absorption.
(2) produce BLP monomer and BLF monomer;
First step salt hydrolysis is told BLP; Add 1.6-2.5% salt solution (sodium chloride brine) from the V2 valve, stirred 40-80 minute, stop to stir and began suction filtration in 3 minutes and separate and tell and through V25, V5, VM5 are transported to the TMF jar, and then through V2 1.6-2.5% salt water wash resin 3 times, leacheate is through V25, and V5, VM5 are discharged to the TMF jar.
Second stage salt hydrolysis is told BLF; Finish the first step from the V2 valve to oneself and separate the resin adding 4-6% salt solution (sodium chloride brine) of telling, stirred 60-120 minute.
The second stage separate tell in, the microfiltration membrane unit is also told liquid to separating of the first step simultaneously and is filtered.Filtrate is through VM2, and V10 is transported to T5.After filter finishing, clean the microfiltration membrane unit and separate and tell liquid to prepare to accept the second stage.
Separate after the time of telling finishes the second stage, BLF is separated tells liquid through V25, after V5, VM5 are drained into the TMF jar, by V 3 valves add RO water wash resin more than 3 times to ionic strength be below the 1.6 milli west doors.Leacheate merges to the TMF jar.Stirred pot T2 recovered to wait upon life state Huaihe River get a collection of production ready the sixth of the twelve Earthly Branches this moment.
Starting the micro-filtration unit filters BLF in the TMF jar and separates and tell liquid.Filtered liquid is through VM2, and V12 is transported to the T6 jar.
Separate at the BLP of T5 and T6 and to tell liquid and BLF and separate and tell liquid successively by ultra-filtration membrane unit desalination and concentration.BLP is transported to drying unit through V17 then.Dried powder is a brown-green.
Then play the resin cation (R.C.) of process in the T7 jar to remove remaining impure milk proem to producing highly purified BLF.The desalting soln of BLF is through V16, and V23 is transported to the T7 jar, stirs absorption after 20-50 minute, and suction filtration liquid is transported to drying unit through V24 and carries out drying.Dry back powder is rose pink.
Embodiment one: 993 liters of semi-skimmed milks, and temperature 8.2 degree C, pH6.6, total protein are every liter of 32.5 gram, 366 milligrams every liter of BLP concentration, BLF concentration are 138 milliliters every liter.Adopt the operational path of Fig. 1, and produce the egg mix white powder of BLP and BLF by the described step of explanation.Dress SPEC70 resin in the stirred pot T2.115 minutes absorption time.Separate and add 90 liters of 5.5% sodium chloride brines when telling, separate the 65 minutes time of telling.Separate and tell and leacheate merges to micro-filtration unit (0.1 micron microfiltration membrane) and carries out the micro-filtration degerming and remove fat.Filtrate is transported to ultra-filtration membrane unit (the 30K molecule is by amount) for 136 liters and carries out desalination and concentration.Receive 0.52 liter of concentrated solution.Adopt freeze-drying method to get light brown green dry powder 351 grams, moisture 4.3%, total protein is 93.2%, ash content 2.6%.
Embodiment two: 996 liters of skimmed milks, and pH6.7, temperature 6.6 degree C total proteins are every liter of 32.9 gram, and BLP concentration is 378 milligrams every liter, and BLF concentration is 129 milligrams every liter.Adopt the operational path of Fig. 1, and produce monomeric BLP albumen and monomeric BLF albumen by the step described in the explanation.SPEC70 resin stirred pot absorption 115 minutes is housed in the adding, and stirring velocity is 60rpm.After collecting the milk of own absorption, with RO water wash resin.Adding first step salt solution is separated for (2.4%) 90 liter and is told 36 minutes, stirring velocity 30rpm.Collection first step BLP separates and tells liquid and add 2.3% salt water wash resin 3 times.The merging leacheate is separated to BLP and is told back microfiltration membrane degerming and fat in the liquid.
At the BLP micro-filtration simultaneously, adding 86 liter of 4.9% salt solution carries out BLF and separates and tell stirring velocity 36rpm in stirred pot.Separate the 60 minutes time of telling.Collection is separated and is told to use RO water wash resin behind the liquid.Merge leacheate to separate tell in the liquid altogether 151 liters.Being transported to the micro-filtration unit carries out degerming and removes fat.Filtrate is transported to ultra filtration unit (30K molecule by amount) and adds water carries out desalination (wash ionic strength and be lower than 1.6 milli west doors) and is concentrated into 1% protein concentration molecule by amount) and add water and carry out desalination (wash ionic strength and be lower than 1.6 milli west doors) and when being concentrated into 1% protein concentration (7.6 liters), being transported to the CM resin container and removing foreigh protein removing.Deliver to after continuing then to be concentrated to 0.39 liter with ultra-filtration membrane the lyophilize unit dry rose pink powder 80.5 grams.Powder is moisture 3.1%, and ash 1.1%, total protein are 96.5%.
BLP behind the micro-filtration continues to concentrate after carrying ultra filtration unit to add water desalination (wash ionic strength and be lower than 1.6 milli west doors).Collect 0.73 liter of BLP concentrated solution.With freeze-drying dry brown-green powder 351 gram, powder is moisture 4.2%, ash 2.1%, total protein are 94.1%.
Claims (10)
1, a kind of method of from milk or whey water, extracting high purity protein, the stirred pot that its adopt to place Zeo-karb adsorbs, desorbs, lactoferrin, newborn Bovinelactoperoxidase and both mixtures thereof in separation and Extraction milk or the whey liquid, and is aided with membrane separation technique and it is separated concentrates and highly purified.
2, method according to claim 1 is characterized in that the adjustable stirring arm rotating speed of stirred pot is 20-100rpm; The jar inner side-wall has the eddy current of increasing baffle plate, and jar end is provided with the resin bed of band screen plate, and 8-12cm highly locates to be provided with quick water conservancy diversion suction filtration pipe above the resin layer on the resin bed.
3, method according to claim 1 is characterized in that being provided with in the jar the various transmitter pH meters of controlling for automatically, the weight meter, and electric conductivity is taken into account thermometer, and the pot liquid holding temperature is 3-160 ℃.
4, method according to claim 1 is characterized in that resin is the SPC70 resin, and number of times that resin absorption desorbs and condition can be set according to the requirement of variant production and product purity; The adsorption time of resin is 20-180 minute, and adsorption temp is at 3-30 ℃.
5, method according to claim 1, the adsorption time that it is characterized in that resin is 35-60 minute.
6, method according to claim 1 is characterized in that the employing two-stage salt concn that desorbs of resin desorbs lactoferrin, newborn Bovinelactoperoxidase.First step salt concn is 1-2.5%, desorbs newborn Bovinelactoperoxidase, and the time of desorbing is 30-100 minute; Second stage salt concn is 3.5-7%, desorbs lactoferrin, and the time of desorbing is 30-100 minute; The salt concn that desorbs newborn Bovinelactoperoxidase and lactoferrin mixture is to be 30-100 minute the 3-7% time of desorbing; The stir speed (S.S.) that desorbs is 20-100rpm.
7, method according to claim 4 is characterized in that it is sodium salt, calcium salt that resin desorbs used salt, sylvite; Resin need not regeneration activating after absorption and desorbing technological process, can be for the usefulness of next batch absorption as long as be lower than 1.6ms with reverse osmosis water (RO water) rinsing resin to its ionic strength after desorbing the technology end.
8, method according to claim 1 is characterized in that microfiltration membrane that membrane separation technique uses the 0.05-0.1 micron with lactoferrin, newborn Bovinelactoperoxidase separate the attached liquid degerming, remove remaining fat and other small-particle; Ultra-filtration membrane with 1K-30K molecular interception amount carries out desalination and concentrated.
9, method according to claim 4 is characterized in that highly purified lactoferrin is that the lactoferrin dope that will desorb desalination and concentration through the absorption of above-mentioned technology is separated the concentrated acquisition that echos ultra-filtration membrane through the absorption of weak-type sun resin such as CM resin again.
10, method according to claim 1, it is characterized in that with adsorb, desorb, the lactoferrin dope of desalination and concentration, breast Bovinelactoperoxidase dope and lactoferrin thereof, newborn Bovinelactoperoxidase mixing dope carries out vacuum dehydrating at lower temperature or low temperature spray drying becomes powder mixture.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101362792B (en) * | 2008-09-25 | 2011-07-20 | 上海交通大学 | Affinity separation polymer of lactoferrin and affinity purification method of lactoferrin |
| CN102898516A (en) * | 2012-10-26 | 2013-01-30 | 浙江大学 | Method for purifying lactoferrin from milk serum by using an expanded bed adsorption technology |
| CN106008703A (en) * | 2016-07-06 | 2016-10-12 | 方雅悯 | Method for extracting and preparing high-purity low-iron-saturation lactoferrin from cow milk |
| CN106377762A (en) * | 2016-08-24 | 2017-02-08 | 方雅悯 | Application of bovine lactoferrin and zymolytes thereof in products for protecting stomach and liver |
| CN109011821A (en) * | 2018-09-18 | 2018-12-18 | 安徽源森生物科技有限公司 | It is a kind of for manufacturing the filter of rice protein |
| CN112979785A (en) * | 2021-04-25 | 2021-06-18 | 黑龙江默赛东科技有限公司 | Method for preparing high-purity lactoferrin |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH0717875A (en) * | 1993-02-09 | 1995-01-20 | Kyodo Nyugyo Kk | Production of iron-lactoferrin powder |
| JP4263932B2 (en) * | 2003-04-01 | 2009-05-13 | 雪印乳業株式会社 | Method for producing lactoferrin |
| CN1319990C (en) * | 2003-08-21 | 2007-06-06 | 安徽农业大学 | Technological process of separating and purifying lactoferritin from cow colostrum |
| CN1557950A (en) * | 2004-02-04 | 2004-12-29 | 高春平 | Method of preparing lactoferritin and lactoperoxidase |
| CN1557494A (en) * | 2004-02-04 | 2004-12-29 | 高春平 | Preparation of microorganism resisting biological active component and its application |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101362792B (en) * | 2008-09-25 | 2011-07-20 | 上海交通大学 | Affinity separation polymer of lactoferrin and affinity purification method of lactoferrin |
| CN102898516A (en) * | 2012-10-26 | 2013-01-30 | 浙江大学 | Method for purifying lactoferrin from milk serum by using an expanded bed adsorption technology |
| CN106008703A (en) * | 2016-07-06 | 2016-10-12 | 方雅悯 | Method for extracting and preparing high-purity low-iron-saturation lactoferrin from cow milk |
| CN106008703B (en) * | 2016-07-06 | 2019-11-22 | 方雅悯 | A method of extracting iron saturation degree lactoferrin low with preparation high-purity from cow's milk |
| CN106377762A (en) * | 2016-08-24 | 2017-02-08 | 方雅悯 | Application of bovine lactoferrin and zymolytes thereof in products for protecting stomach and liver |
| CN109011821A (en) * | 2018-09-18 | 2018-12-18 | 安徽源森生物科技有限公司 | It is a kind of for manufacturing the filter of rice protein |
| CN112979785A (en) * | 2021-04-25 | 2021-06-18 | 黑龙江默赛东科技有限公司 | Method for preparing high-purity lactoferrin |
| CN112979785B (en) * | 2021-04-25 | 2021-12-28 | 黑龙江默赛东科技有限公司 | Method for preparing high-purity lactoferrin |
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